srp68 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following srp68 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the srp68 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSj00729 NM_001022791.2
Latest version!
Schizosaccharomyces pombe 972h- signal recognition particle subunit (predicted) (srp68), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OSc99337 NM_001355875.1
Latest version!
Schizosaccharomyces pombe putative signal recognition particle subunit protein (srp68), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSj00729
    Clone ID Related Accession (Same CDS sequence) NM_001022791.2
    Accession Version NM_001022791.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1794bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product signal recognition particle subunit (predicted)
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022791.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACCCGC ATGTTAAGCT TTCAACGTCC TTGGTAATAA TAGCTTTTAG ATATTTACCA 
    AATAATTTTA ACGATTCACG CCTAGGATGC GATAATCTAA GCACCGTTGG TTTATGTCTC
    AAAAATGGCA CCGGCGAAGT ACTGTTTTCT GGTTTGATTA AGCAAATGGC TAATTTTTAT
    ATTTTTCCTT TATTACTGGA AGCCAGATCT GATCATTTTT ATGAAGGAAA TGAGTACATA
    AAATATTTGT CCCATCGCAT TCATGGATTA CGAAAATCCC TTCATATTAC ACAGAGAGGT
    GGGAAACCAA AATCTTCAGT AGACAAAAGA TATGCTGAAA TTTTACTTTT CAATGCTGAT
    CGTGCTTTTC AACAATTTGT TTTTTTGCGA TCTAGTCAAC GTCGACATGC ATTGCGCAGA
    TTAAAACGTG CAGATCAATT TGGAAAAGAG TTGGTTTCCT TTACGAATGC ATTTGATTGC
    AACGATCATA TCTTTTATTT GGAGGCTATT GCTTTTGCCA AATATATCGA GGGTACTCTA
    AACTACGAGA AACGAGATTG GGAAGGCTCT TTGAGCGCTT TTAGTATTTC TCGACTTTCA
    TTCCTAGTCT TGCAAAATAA AATTGATACA CTTGCTGAAC ATGAGAAGAG TGTACTTGGA
    GAACTCCAAA ATCAAATTGA TTCCAATTTA CGATACGTTG CTCAAAGAAC TGGCTTGCAA
    AACCAGACCA AGTCATTAGA CATTTTGATG TTGTCAAGTA TACCGAAGGA TGAGGAGGTA
    ATTCAGCATG TAAATTCCGT TGATTCTGAA ATATTGCAAA TGACCGGAGA TGAACAAGAT
    TCCTTGCAAA CTATCACAGT CATTGAATGG AGAGATCAGA GAGTAAAAAT AGAGCATCCT
    GATGTCGTAT TGGCTCTATA TGCAATTCAC GATGTCAAAA ATTCTCCTGG AACCATAGAC
    TCTAAAAGAG ATCGTCTGCT TGCTGCATGG GCTAGAGCAG AAGAAATTAC CAAAAGCGTT
    CTTGATAACA CAGGCCTTGA AGACGAGCAA AAATTCCAAA CTCTCTCTAT TTGTTATACT
    TACTTGGCTT ACAATGTAGT ATTGCTCCGT ATCCAAAGAG ACTTGGCAGT TGAGAATGAT
    TCCGAATTGG TTGCTTCACA AGCTCAGCTT CGATCTCGTC AAAGCTTGTA TGATTCTATT
    ATTAAGAACA TTGAAATTGC TAAAGAATTG CCCGGTATTG CACGAGATAC TGGTATGACT
    GCCCAACTAG AAGCCCAAAT TTCTTTGGCT AAGAGTAAAC GCTGCCAGGC TATTGCCGAT
    GCTTACCAGG CACAGGATAA ACTGGCGAGT CTTGCGATGT GTGTACGAGC TGCCTCGTAT
    TTACAACAGG TGAATGATAT ATTGCGCAAC TTTGAAGAAA AGCCACATCT TATAGCATTC
    GATATAATCC CTGAACTTAA AAAAGACGAA AAGGAGCTAA AAAAGAAAAT TTTGCTATTA
    CAAAGTTTGG CATCCATGGG AAATATAAAT CAACCACCTA AAAACTTGTC GTTGGTTGAA
    ACACTTGATA GTTATCAAAC GCTTGCAGAA TTAGAACCAT CTTGGAATCT TACAGACGCT
    GATATTAGGG CTATTCCAGC AAAGCCTTTA TTCTTTGATT TAGCTATTAC ATATCTAGGT
    CAGCAGACCT CTTTTGACAA AAAGAAAGCA CAACCGGAAA AACCTGTTAC ATCAGTTTCG
    AAAGAGCCTA AACAGAAAAA TAAAGGGTTC TTTAGCAGTT TGCTTGGACG ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_587798.1
    CDS1..1794
    Translation

    Target ORF information:

    RefSeq Version NM_001022791.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- signal recognition particle subunit (predicted) (srp68), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022791.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACCCGC ATGTTAAGCT TTCAACGTCC TTGGTAATAA TAGCTTTTAG ATATTTACCA 
    AATAATTTTA ACGATTCACG CCTAGGATGC GATAATCTAA GCACCGTTGG TTTATGTCTC
    AAAAATGGCA CCGGCGAAGT ACTGTTTTCT GGTTTGATTA AGCAAATGGC TAATTTTTAT
    ATTTTTCCTT TATTACTGGA AGCCAGATCT GATCATTTTT ATGAAGGAAA TGAGTACATA
    AAATATTTGT CCCATCGCAT TCATGGATTA CGAAAATCCC TTCATATTAC ACAGAGAGGT
    GGGAAACCAA AATCTTCAGT AGACAAAAGA TATGCTGAAA TTTTACTTTT CAATGCTGAT
    CGTGCTTTTC AACAATTTGT TTTTTTGCGA TCTAGTCAAC GTCGACATGC ATTGCGCAGA
    TTAAAACGTG CAGATCAATT TGGAAAAGAG TTGGTTTCCT TTACGAATGC ATTTGATTGC
    AACGATCATA TCTTTTATTT GGAGGCTATT GCTTTTGCCA AATATATCGA GGGTACTCTA
    AACTACGAGA AACGAGATTG GGAAGGCTCT TTGAGCGCTT TTAGTATTTC TCGACTTTCA
    TTCCTAGTCT TGCAAAATAA AATTGATACA CTTGCTGAAC ATGAGAAGAG TGTACTTGGA
    GAACTCCAAA ATCAAATTGA TTCCAATTTA CGATACGTTG CTCAAAGAAC TGGCTTGCAA
    AACCAGACCA AGTCATTAGA CATTTTGATG TTGTCAAGTA TACCGAAGGA TGAGGAGGTA
    ATTCAGCATG TAAATTCCGT TGATTCTGAA ATATTGCAAA TGACCGGAGA TGAACAAGAT
    TCCTTGCAAA CTATCACAGT CATTGAATGG AGAGATCAGA GAGTAAAAAT AGAGCATCCT
    GATGTCGTAT TGGCTCTATA TGCAATTCAC GATGTCAAAA ATTCTCCTGG AACCATAGAC
    TCTAAAAGAG ATCGTCTGCT TGCTGCATGG GCTAGAGCAG AAGAAATTAC CAAAAGCGTT
    CTTGATAACA CAGGCCTTGA AGACGAGCAA AAATTCCAAA CTCTCTCTAT TTGTTATACT
    TACTTGGCTT ACAATGTAGT ATTGCTCCGT ATCCAAAGAG ACTTGGCAGT TGAGAATGAT
    TCCGAATTGG TTGCTTCACA AGCTCAGCTT CGATCTCGTC AAAGCTTGTA TGATTCTATT
    ATTAAGAACA TTGAAATTGC TAAAGAATTG CCCGGTATTG CACGAGATAC TGGTATGACT
    GCCCAACTAG AAGCCCAAAT TTCTTTGGCT AAGAGTAAAC GCTGCCAGGC TATTGCCGAT
    GCTTACCAGG CACAGGATAA ACTGGCGAGT CTTGCGATGT GTGTACGAGC TGCCTCGTAT
    TTACAACAGG TGAATGATAT ATTGCGCAAC TTTGAAGAAA AGCCACATCT TATAGCATTC
    GATATAATCC CTGAACTTAA AAAAGACGAA AAGGAGCTAA AAAAGAAAAT TTTGCTATTA
    CAAAGTTTGG CATCCATGGG AAATATAAAT CAACCACCTA AAAACTTGTC GTTGGTTGAA
    ACACTTGATA GTTATCAAAC GCTTGCAGAA TTAGAACCAT CTTGGAATCT TACAGACGCT
    GATATTAGGG CTATTCCAGC AAAGCCTTTA TTCTTTGATT TAGCTATTAC ATATCTAGGT
    CAGCAGACCT CTTTTGACAA AAAGAAAGCA CAACCGGAAA AACCTGTTAC ATCAGTTTCG
    AAAGAGCCTA AACAGAAAAA TAAAGGGTTC TTTAGCAGTT TGCTTGGACG ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSc99337
    Clone ID Related Accession (Same CDS sequence) NM_001355875.1
    Accession Version NM_001355875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1794bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative signal recognition particle subunit protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACCCGC ATGTTAAGCT TTCAACGTCC TTGGTAATAA TAGCTTTTAG ATATTTACCA 
    AATAATTTTA ACGATTCACG CCTAGGATGC GATAATCTAA GCACCGTTGG TTTATGTCTC
    AAAAATGGCA CCGGCGAAGT ACTGTTTTCT GGTTTGATTA AGCAAATGGC TAATTTTTAT
    ATTTTTCCTT TATTACTGGA AGCCAGATCT GATCATTTTT ATGAAGGAAA TGAGTACATA
    AAATATTTGT CCCATCGCAT TCATGGATTA CGAAAATCCC TTCATATTAC ACAGAGAGGT
    GGGAAACCAA AATCTTCAGT AGACAAAAGA TATGCTGAAA TTTTACTTTT CAATGCTGAT
    CGTGCTTTTC AACAATTTGT TTTTTTGCGA TCTAGTCAAC GTCGACATGC ATTGCGCAGA
    TTAAAACGTG CAGATCAATT TGGAAAAGAG TTGGTTTCCT TTACGAATGC ATTTGATTGC
    AACGATCATA TCTTTTATTT GGAGGCTATT GCTTTTGCCA AATATATCGA GGGTACTCTA
    AACTACGAGA AACGAGATTG GGAAGGCTCT TTGAGCGCTT TTAGTATTTC TCGACTTTCA
    TTCCTAGTCT TGCAAAATAA AATTGATACA CTTGCTGAAC ATGAGAAGAG TGTACTTGGA
    GAACTCCAAA ATCAAATTGA TTCCAATTTA CGATACGTTG CTCAAAGAAC TGGCTTGCAA
    AACCAGACCA AGTCATTAGA CATTTTGATG TTGTCAAGTA TACCGAAGGA TGAGGAGGTA
    ATTCAGCATG TAAATTCCGT TGATTCTGAA ATATTGCAAA TGACCGGAGA TGAACAAGAT
    TCCTTGCAAA CTATCACAGT CATTGAATGG AGAGATCAGA GAGTAAAAAT AGAGCATCCT
    GATGTCGTAT TGGCTCTATA TGCAATTCAC GATGTCAAAA ATTCTCCTGG AACCATAGAC
    TCTAAAAGAG ATCGTCTGCT TGCTGCATGG GCTAGAGCAG AAGAAATTAC CAAAAGCGTT
    CTTGATAACA CAGGCCTTGA AGACGAGCAA AAATTCCAAA CTCTCTCTAT TTGTTATACT
    TACTTGGCTT ACAATGTAGT ATTGCTCCGT ATCCAAAGAG ACTTGGCAGT TGAGAATGAT
    TCCGAATTGG TTGCTTCACA AGCTCAGCTT CGATCTCGTC AAAGCTTGTA TGATTCTATT
    ATTAAGAACA TTGAAATTGC TAAAGAATTG CCCGGTATTG CACGAGATAC TGGTATGACT
    GCCCAACTAG AAGCCCAAAT TTCTTTGGCT AAGAGTAAAC GCTGCCAGGC TATTGCCGAT
    GCTTACCAGG CACAGGATAA ACTGGCGAGT CTTGCGATGT GTGTACGAGC TGCCTCGTAT
    TTACAACAGG TGAATGATAT ATTGCGCAAC TTTGAAGAAA AGCCACATCT TATAGCATTC
    GATATAATCC CTGAACTTAA AAAAGACGAA AAGGAGCTAA AAAAGAAAAT TTTGCTATTA
    CAAAGTTTGG CATCCATGGG AAATATAAAT CAACCACCTA AAAACTTGTC GTTGGTTGAA
    ACACTTGATA GTTATCAAAC GCTTGCAGAA TTAGAACCAT CTTGGAATCT TACAGACGCT
    GATATTAGGG CTATTCCAGC AAAGCCTTTA TTCTTTGATT TAGCTATTAC ATATCTAGGT
    CAGCAGACCT CTTTTGACAA AAAGAAAGCA CAACCGGAAA AACCTGTTAC ATCAGTTTCG
    AAAGAGCCTA AACAGAAAAA TAAAGGGTTC TTTAGCAGTT TGCTTGGACG ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342750.1
    CDS1..1794
    Translation

    Target ORF information:

    RefSeq Version NM_001355875.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative signal recognition particle subunit protein (srp68), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACCCGC ATGTTAAGCT TTCAACGTCC TTGGTAATAA TAGCTTTTAG ATATTTACCA 
    AATAATTTTA ACGATTCACG CCTAGGATGC GATAATCTAA GCACCGTTGG TTTATGTCTC
    AAAAATGGCA CCGGCGAAGT ACTGTTTTCT GGTTTGATTA AGCAAATGGC TAATTTTTAT
    ATTTTTCCTT TATTACTGGA AGCCAGATCT GATCATTTTT ATGAAGGAAA TGAGTACATA
    AAATATTTGT CCCATCGCAT TCATGGATTA CGAAAATCCC TTCATATTAC ACAGAGAGGT
    GGGAAACCAA AATCTTCAGT AGACAAAAGA TATGCTGAAA TTTTACTTTT CAATGCTGAT
    CGTGCTTTTC AACAATTTGT TTTTTTGCGA TCTAGTCAAC GTCGACATGC ATTGCGCAGA
    TTAAAACGTG CAGATCAATT TGGAAAAGAG TTGGTTTCCT TTACGAATGC ATTTGATTGC
    AACGATCATA TCTTTTATTT GGAGGCTATT GCTTTTGCCA AATATATCGA GGGTACTCTA
    AACTACGAGA AACGAGATTG GGAAGGCTCT TTGAGCGCTT TTAGTATTTC TCGACTTTCA
    TTCCTAGTCT TGCAAAATAA AATTGATACA CTTGCTGAAC ATGAGAAGAG TGTACTTGGA
    GAACTCCAAA ATCAAATTGA TTCCAATTTA CGATACGTTG CTCAAAGAAC TGGCTTGCAA
    AACCAGACCA AGTCATTAGA CATTTTGATG TTGTCAAGTA TACCGAAGGA TGAGGAGGTA
    ATTCAGCATG TAAATTCCGT TGATTCTGAA ATATTGCAAA TGACCGGAGA TGAACAAGAT
    TCCTTGCAAA CTATCACAGT CATTGAATGG AGAGATCAGA GAGTAAAAAT AGAGCATCCT
    GATGTCGTAT TGGCTCTATA TGCAATTCAC GATGTCAAAA ATTCTCCTGG AACCATAGAC
    TCTAAAAGAG ATCGTCTGCT TGCTGCATGG GCTAGAGCAG AAGAAATTAC CAAAAGCGTT
    CTTGATAACA CAGGCCTTGA AGACGAGCAA AAATTCCAAA CTCTCTCTAT TTGTTATACT
    TACTTGGCTT ACAATGTAGT ATTGCTCCGT ATCCAAAGAG ACTTGGCAGT TGAGAATGAT
    TCCGAATTGG TTGCTTCACA AGCTCAGCTT CGATCTCGTC AAAGCTTGTA TGATTCTATT
    ATTAAGAACA TTGAAATTGC TAAAGAATTG CCCGGTATTG CACGAGATAC TGGTATGACT
    GCCCAACTAG AAGCCCAAAT TTCTTTGGCT AAGAGTAAAC GCTGCCAGGC TATTGCCGAT
    GCTTACCAGG CACAGGATAA ACTGGCGAGT CTTGCGATGT GTGTACGAGC TGCCTCGTAT
    TTACAACAGG TGAATGATAT ATTGCGCAAC TTTGAAGAAA AGCCACATCT TATAGCATTC
    GATATAATCC CTGAACTTAA AAAAGACGAA AAGGAGCTAA AAAAGAAAAT TTTGCTATTA
    CAAAGTTTGG CATCCATGGG AAATATAAAT CAACCACCTA AAAACTTGTC GTTGGTTGAA
    ACACTTGATA GTTATCAAAC GCTTGCAGAA TTAGAACCAT CTTGGAATCT TACAGACGCT
    GATATTAGGG CTATTCCAGC AAAGCCTTTA TTCTTTGATT TAGCTATTAC ATATCTAGGT
    CAGCAGACCT CTTTTGACAA AAAGAAAGCA CAACCGGAAA AACCTGTTAC ATCAGTTTCG
    AAAGAGCCTA AACAGAAAAA TAAAGGGTTC TTTAGCAGTT TGCTTGGACG ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L