ucp12 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following ucp12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ucp12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99634 NM_001023042.2
Latest version!
Schizosaccharomyces pombe putative ATP-dependent RNA helicase Ucp12 (ucp12), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99634
    Clone ID Related Accession (Same CDS sequence) NM_001023042.2
    Accession Version NM_001023042.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative ATP-dependent RNA helicase Ucp12
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023042.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGTTCAA AAGGAAAAAA GGGAAAATCA TCGGAGGTTA ATTTAGAAAC TTCAAAAAAC 
    AAGGAAAAAA ATATCAAAGG GAAAAAGAAA AACTCACTTG ATCCAATTGA AAAAAATAAA
    CAGGAAACAG CAGGCCTGCA AACGACTTCA CGTCCTACTG CCAAACAGTT AGTAGGAGGA
    AGCTCTTGGA CTGGAAAAAT TCCTGTCGTT TTACTAAATG AACATTGCCA AAGAAGTAAA
    TGGGAGAAAA GTGATGTTAA GGTGAGGCAA ACCAAATCTA AAAATTACAT TTTTACTTCT
    GTCGTACTTG CTAAGAAGGA CCCAAAAAAC CCTTCGATAT TAGACCATGT ATCTCTGATT
    CCTCCCAAGT CCTATTATGA GAATGGCTAC TTTCCTGAAA AAGAAACGTT AGTGGAAGCA
    CGAAATGTTG GCGCCGTTTA TGCTTTGCAT CGAATAATGT CTCATAAATC ATTACAACAT
    GCCCTTCCAC CTGAACATCG GAATATATGG TTTGATATGG AGAAACAAAA GAAGGAAGAG
    CTAAAAAATA AGCACAGCTG GCTTTACAAT GAAGATCCCT TCAAAGCAGC CAAAGAACTA
    CAGGCAGCAC GCGCTTCTTC AGCTGCCAAA CCACCGCCAA AAGCATCTCA GAAAAATGAA
    AAGGTCTCTT TGACATCTAT CAAAAACACA AGCCTTTCAC ACTTCTCAAA GTTCAATTTC
    AAATATGCTC TTCCTATTCA CATGAGTCTT GAAAATCGTC GCTCTCTAGA GAACCTTTTT
    CGCAATATGA ACACGTGGGA TATACTAGAG GATACAAAAA ATTTAGAGCC CGATACTTCT
    ATTGTTAATG ATTTAATTTC TTTAGGTTTT CGAGACATTC ATGCTAAAGA AGCTTGTCAG
    TACTGTGTTT CCCTTGAAGA TGCTTTAGAG TGGCTAATCA TTCATGTTCC AGAAGACGAT
    CTTCCTACAA GATTTCTTCC TTCTGATTAT ACAACAGGAA TATCTGTTCA GAATCTAAAT
    TCAGCGAATC TGGCCATTCA CTACAACGCT AAACGGATTT CGGAGACGGG CTATAGCTTT
    GACTTATGTT TTTCTACTCT TCAAACTTTT GAAAATAACA TTCAAATTAG TTCAGAGTAC
    TTACAGCAGC ATCTAATTGG TGAAAGCTTT GATGGGAATA TATCTTTGGA ACCAAATAGT
    ACCGAATGGG ATGATGATGT TAGTGCTTTG CAATCTATTT TGGATAATAA GGTCTCAAAA
    ATAGAGAATG GATGTAGAGT GCGTATTGAC TACCCGACTA GTGAATTTGG TGAGCTTTTC
    GTCGATTTTA GAAGGCCTGC CCGTTCGTAT CCTGCACACA TTCCTTTAAT GTCTCTATCC
    TCTACAAAAA GAATGGCATC CTATATTAAG TTGAGTATTC TTAAAAAGAT GGTTGTTTAC
    GCAATGGATC TTAGAGGTGA ATGCATGTTA TCCTGGTTGT ATAATCATCT TCAGGAAAAT
    ATTGAAGACT TTTTACAAAA TATTGGAAGC TTACTAAATA TTTCAGCTGC AACAATTGGT
    GTCTCTTTAT CCTCTCAAAA CAAGTCTGCG CCGACTGCCA AAAAGAATAA CAGTTTCAAG
    CCAAAATTAT TTCGCCGTTC GCGCGAATTA TCTGAAAAAT TATGTAATAA CTGGAGTGAG
    AGGGTTAAAA GTCCTTCATA TCAATTAAAA GTTCGTGAAC GTGAGAAACT CCCTGCATGG
    GAATCAAGGA GAAAAATTAT GGACGCCATA CAGCATTCTC AAGTTGTTGT TATAAGTGGT
    GAAACTGGTA GTGGAAAATC AACACAAGTA GTTCAATTTA TCTTGGACCA TTATCTTTCT
    TCTGGAGAAA AAGATTTGCA AACAGTTGTT TGTACACAGC CAAGGAGAAT ATCTGCGATA
    AGTTTGGCGG AAAGAGTTGC CTTCGAACGT GATACAACTG TTGGAAAAGA AGTTGGTTAC
    AGTGTACATG GTGAAAAATC CATATCTAAA GAAACCCTTC TTGAGTTTTG CACTACAGGT
    TTGCTTCTTA GAAGAATTCA GCAACATGGT CTAGGCTTTT TGAGTACTTT ATCTTGTGTT
    GTTGTAGATG AAGTACATGA AAGATCCATC GAAAATGATA TACTCTTAAC ACTTTTGAAA
    CTAGTAATTT CTCGTATCCC AAATTTGAAA GTCATTTTGA TGAGTGCCAC GGTGAACTCC
    GATACATTCA AATATTATTT TGGAAATGCT GGTCATTTAC ATATTCACGG CCGTACTTTT
    CCGATCAAGG ATTACTACAT TGAGGATTTT GCGCCTAAGC TAAATGAAGA TGATGACGAG
    GAAGACGTTC CCAGAAGAAA GAAAAAAGAA TATGAAATTG ATTATCATCT TATTTCTAGA
    CTTGTTAGTT CTATTGATGC TGAACTTGGT TCCTCTAGTG GTTCTATATT GGTTTTCCTT
    CCCGGTGTCT CAAACATAGC TCGTTGTATT CGTGAAATAA AATCAAAAGA CGGCTCAAAA
    TTTGAGGTCC TCCCATTACA TGCCTCTTTG AATACTTCGG AGCAAAGGAG ATGTTTCAAA
    ACATACACAA AGCGGAAGAT CATATGTGCA ACAAATATTG CGGAGACTTC AATTACTATT
    GATGATGTTG TTGCCGTTAT TGACAGCGGC AGAGTTAAGC AAATTGATTA TGATGTTGAA
    CGAGATCTGG TGACTTTTAA AGAGACTTGG GCCTCAAGAG CTGCTTGTCA ACAACGCCGG
    GGTCGGGCGG GTCGTGTTAA GAAAGGAATA TGTTATAAGT TATATACAAG AGGATTTGAA
    GAAAAAGGAA TGCTAGGCCA AACACCTCCC GAAGTTTTAC GGACGGCTTT AAGTCAGGTT
    TGTCTTAACG TAGTTCCTTT GGTCAAGAGA TTTTCTTCTG CTGGTAATTC TGTGAACCAG
    GGTTCAATCA AAAAATTTAT GAACAGCCTT ATTGATCCAC CGAATGATGC AACGGTAGAC
    CTTGCTTTGA AAAAACTAAT TCAAGTCGGT GCTCTCACCG TGTCAGAAGA TTTAACTGGT
    TTAGGAGAAT ACTTAGTGTC ATTACCCATA GATCTAAAGC TTGGAAAGCT TTTGGTGTTT
    GGTTCGATCT TTGGATATTT GGAACCTGCG CTTACTATTA CAGCTATTCT CTCTACAAAG
    TCTCCTTTCT TAGGAGACGA TGAAGCTAGG GAAATAAGGA GTAAACAAAG TCAAGGATGG
    GGAGATGTTT TGGCTGATGC TCGAGTATAT CACAACTGGC TCGAAATACT AGAAACCAGG
    GGTGTAAAGA AAACAGTTCA GTGGTGTGAA GAGATGCATC TTCATTACAC AACGTTACAA
    CAAATTCGAC AGAATCGAAA CGAATTGAGC GAAGCTGCAC AGCTACTTGA ACTAACTACT
    AAAAAGCTGA CTGGTAACTT TGATTGGTAT TCAACTGAAA ACCTGACTGT TCTATCGACT
    CTAATTGCTG CTGCTTTAAG TCCCAATGTT GTGAAATGTG TTTATCCTGA TAAAAAATTT
    GTTGCTTCAT TTTCTGGATC GCTTGAAATG GAGCAGGAAG CTCGCCTGAC GAAGTTTTAT
    GATCAAAACA ACCAAAGGTT ATTCATACAT CCTTCAAGTA CTATGTTTGT TAATTCTCCA
    AATGCTTCAA GATGTACGTT CGTGGCATAT GAGCAAAAGG TAGAAACCAC GAAACCTTTC
    CTTAGAAATT GTACACCTAT CAATACATAT GGAATGATTT TGCTGGGAGC AAATGATATA
    CTCATTGATC CTCTTGGTAA GGGATTAATA CTTGATCAAG CATATTGCAT TAAAGCGTGG
    CCTAAAGTCG TTATTTTATT AAAAATGCTA AAAAGATGTC TTGATGCCTC ATTACATGAG
    CGATTGGAAT CTTCGTCGGG GTTAAACTAC GAAAGTGAAA TACATCAATG TATTCGTACT
    CTGATTGCAG GCAACGGTGT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_588050.1
    CDS1146..5129
    Translation

    Target ORF information:

    RefSeq Version NM_001023042.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative ATP-dependent RNA helicase Ucp12 (ucp12), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001023042.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGTTCAA AAGGAAAAAA GGGAAAATCA TCGGAGGTTA ATTTAGAAAC TTCAAAAAAC 
    AAGGAAAAAA ATATCAAAGG GAAAAAGAAA AACTCACTTG ATCCAATTGA AAAAAATAAA
    CAGGAAACAG CAGGCCTGCA AACGACTTCA CGTCCTACTG CCAAACAGTT AGTAGGAGGA
    AGCTCTTGGA CTGGAAAAAT TCCTGTCGTT TTACTAAATG AACATTGCCA AAGAAGTAAA
    TGGGAGAAAA GTGATGTTAA GGTGAGGCAA ACCAAATCTA AAAATTACAT TTTTACTTCT
    GTCGTACTTG CTAAGAAGGA CCCAAAAAAC CCTTCGATAT TAGACCATGT ATCTCTGATT
    CCTCCCAAGT CCTATTATGA GAATGGCTAC TTTCCTGAAA AAGAAACGTT AGTGGAAGCA
    CGAAATGTTG GCGCCGTTTA TGCTTTGCAT CGAATAATGT CTCATAAATC ATTACAACAT
    GCCCTTCCAC CTGAACATCG GAATATATGG TTTGATATGG AGAAACAAAA GAAGGAAGAG
    CTAAAAAATA AGCACAGCTG GCTTTACAAT GAAGATCCCT TCAAAGCAGC CAAAGAACTA
    CAGGCAGCAC GCGCTTCTTC AGCTGCCAAA CCACCGCCAA AAGCATCTCA GAAAAATGAA
    AAGGTCTCTT TGACATCTAT CAAAAACACA AGCCTTTCAC ACTTCTCAAA GTTCAATTTC
    AAATATGCTC TTCCTATTCA CATGAGTCTT GAAAATCGTC GCTCTCTAGA GAACCTTTTT
    CGCAATATGA ACACGTGGGA TATACTAGAG GATACAAAAA ATTTAGAGCC CGATACTTCT
    ATTGTTAATG ATTTAATTTC TTTAGGTTTT CGAGACATTC ATGCTAAAGA AGCTTGTCAG
    TACTGTGTTT CCCTTGAAGA TGCTTTAGAG TGGCTAATCA TTCATGTTCC AGAAGACGAT
    CTTCCTACAA GATTTCTTCC TTCTGATTAT ACAACAGGAA TATCTGTTCA GAATCTAAAT
    TCAGCGAATC TGGCCATTCA CTACAACGCT AAACGGATTT CGGAGACGGG CTATAGCTTT
    GACTTATGTT TTTCTACTCT TCAAACTTTT GAAAATAACA TTCAAATTAG TTCAGAGTAC
    TTACAGCAGC ATCTAATTGG TGAAAGCTTT GATGGGAATA TATCTTTGGA ACCAAATAGT
    ACCGAATGGG ATGATGATGT TAGTGCTTTG CAATCTATTT TGGATAATAA GGTCTCAAAA
    ATAGAGAATG GATGTAGAGT GCGTATTGAC TACCCGACTA GTGAATTTGG TGAGCTTTTC
    GTCGATTTTA GAAGGCCTGC CCGTTCGTAT CCTGCACACA TTCCTTTAAT GTCTCTATCC
    TCTACAAAAA GAATGGCATC CTATATTAAG TTGAGTATTC TTAAAAAGAT GGTTGTTTAC
    GCAATGGATC TTAGAGGTGA ATGCATGTTA TCCTGGTTGT ATAATCATCT TCAGGAAAAT
    ATTGAAGACT TTTTACAAAA TATTGGAAGC TTACTAAATA TTTCAGCTGC AACAATTGGT
    GTCTCTTTAT CCTCTCAAAA CAAGTCTGCG CCGACTGCCA AAAAGAATAA CAGTTTCAAG
    CCAAAATTAT TTCGCCGTTC GCGCGAATTA TCTGAAAAAT TATGTAATAA CTGGAGTGAG
    AGGGTTAAAA GTCCTTCATA TCAATTAAAA GTTCGTGAAC GTGAGAAACT CCCTGCATGG
    GAATCAAGGA GAAAAATTAT GGACGCCATA CAGCATTCTC AAGTTGTTGT TATAAGTGGT
    GAAACTGGTA GTGGAAAATC AACACAAGTA GTTCAATTTA TCTTGGACCA TTATCTTTCT
    TCTGGAGAAA AAGATTTGCA AACAGTTGTT TGTACACAGC CAAGGAGAAT ATCTGCGATA
    AGTTTGGCGG AAAGAGTTGC CTTCGAACGT GATACAACTG TTGGAAAAGA AGTTGGTTAC
    AGTGTACATG GTGAAAAATC CATATCTAAA GAAACCCTTC TTGAGTTTTG CACTACAGGT
    TTGCTTCTTA GAAGAATTCA GCAACATGGT CTAGGCTTTT TGAGTACTTT ATCTTGTGTT
    GTTGTAGATG AAGTACATGA AAGATCCATC GAAAATGATA TACTCTTAAC ACTTTTGAAA
    CTAGTAATTT CTCGTATCCC AAATTTGAAA GTCATTTTGA TGAGTGCCAC GGTGAACTCC
    GATACATTCA AATATTATTT TGGAAATGCT GGTCATTTAC ATATTCACGG CCGTACTTTT
    CCGATCAAGG ATTACTACAT TGAGGATTTT GCGCCTAAGC TAAATGAAGA TGATGACGAG
    GAAGACGTTC CCAGAAGAAA GAAAAAAGAA TATGAAATTG ATTATCATCT TATTTCTAGA
    CTTGTTAGTT CTATTGATGC TGAACTTGGT TCCTCTAGTG GTTCTATATT GGTTTTCCTT
    CCCGGTGTCT CAAACATAGC TCGTTGTATT CGTGAAATAA AATCAAAAGA CGGCTCAAAA
    TTTGAGGTCC TCCCATTACA TGCCTCTTTG AATACTTCGG AGCAAAGGAG ATGTTTCAAA
    ACATACACAA AGCGGAAGAT CATATGTGCA ACAAATATTG CGGAGACTTC AATTACTATT
    GATGATGTTG TTGCCGTTAT TGACAGCGGC AGAGTTAAGC AAATTGATTA TGATGTTGAA
    CGAGATCTGG TGACTTTTAA AGAGACTTGG GCCTCAAGAG CTGCTTGTCA ACAACGCCGG
    GGTCGGGCGG GTCGTGTTAA GAAAGGAATA TGTTATAAGT TATATACAAG AGGATTTGAA
    GAAAAAGGAA TGCTAGGCCA AACACCTCCC GAAGTTTTAC GGACGGCTTT AAGTCAGGTT
    TGTCTTAACG TAGTTCCTTT GGTCAAGAGA TTTTCTTCTG CTGGTAATTC TGTGAACCAG
    GGTTCAATCA AAAAATTTAT GAACAGCCTT ATTGATCCAC CGAATGATGC AACGGTAGAC
    CTTGCTTTGA AAAAACTAAT TCAAGTCGGT GCTCTCACCG TGTCAGAAGA TTTAACTGGT
    TTAGGAGAAT ACTTAGTGTC ATTACCCATA GATCTAAAGC TTGGAAAGCT TTTGGTGTTT
    GGTTCGATCT TTGGATATTT GGAACCTGCG CTTACTATTA CAGCTATTCT CTCTACAAAG
    TCTCCTTTCT TAGGAGACGA TGAAGCTAGG GAAATAAGGA GTAAACAAAG TCAAGGATGG
    GGAGATGTTT TGGCTGATGC TCGAGTATAT CACAACTGGC TCGAAATACT AGAAACCAGG
    GGTGTAAAGA AAACAGTTCA GTGGTGTGAA GAGATGCATC TTCATTACAC AACGTTACAA
    CAAATTCGAC AGAATCGAAA CGAATTGAGC GAAGCTGCAC AGCTACTTGA ACTAACTACT
    AAAAAGCTGA CTGGTAACTT TGATTGGTAT TCAACTGAAA ACCTGACTGT TCTATCGACT
    CTAATTGCTG CTGCTTTAAG TCCCAATGTT GTGAAATGTG TTTATCCTGA TAAAAAATTT
    GTTGCTTCAT TTTCTGGATC GCTTGAAATG GAGCAGGAAG CTCGCCTGAC GAAGTTTTAT
    GATCAAAACA ACCAAAGGTT ATTCATACAT CCTTCAAGTA CTATGTTTGT TAATTCTCCA
    AATGCTTCAA GATGTACGTT CGTGGCATAT GAGCAAAAGG TAGAAACCAC GAAACCTTTC
    CTTAGAAATT GTACACCTAT CAATACATAT GGAATGATTT TGCTGGGAGC AAATGATATA
    CTCATTGATC CTCTTGGTAA GGGATTAATA CTTGATCAAG CATATTGCAT TAAAGCGTGG
    CCTAAAGTCG TTATTTTATT AAAAATGCTA AAAAGATGTC TTGATGCCTC ATTACATGAG
    CGATTGGAAT CTTCGTCGGG GTTAAACTAC GAAAGTGAAA TACATCAATG TATTCGTACT
    CTGATTGCAG GCAACGGTGT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L