SPCC18.13 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPCC18.13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPCC18.13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99521 NM_001023383.2
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Schizosaccharomyces pombe tRNA (guanine-N7-)-methyltransferase subunit Trm82 (SPCC18.13), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99521
    Clone ID Related Accession (Same CDS sequence) NM_001023383.2
    Accession Version NM_001023383.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product tRNA (guanine-N7-)-methyltransferase subunit Trm82
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023383.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGTGAAC AGCGAGTTTT TAAGCACCCT TGTCAATTTT TAACATGGAA TTCTAAACAC 
    AATTACATCG TGTGCTGTTC AGGTCCATAT CTATTGGGGT TCTCATGCTC TACAGGGGAA
    AAAATTTTTG AACATTGTTA TAGAGACAAT ATAAATGAAA AACATAGAGA GGCAGCTGCC
    TATGGAGAAG CGATTCGTCA AGTCGCATTT TCTAAAGACT ATTCTCGTAT GGCAACAGTT
    AGTGAAGACA AATGTTTGCG CCTTTGGGAT AGCACTCAGC CTGATAAAAT TGAACTTCTT
    TATCAAAAAA ACATACCAAA GCGATGTGCT GACTTGTGCT TTGCGGGCTC AAACGAAATT
    GTTTTTGGTG ATAAGTTTGG TGATGTCTAT TGTGTAGACG AAAATTGGTT TACTACTTCT
    GAAGTTACCG AAGAAAAAAA GTCAAATGTG GTTGAAGGAA AACAAGAACC TGTTAATAAT
    GACACTTTAA AGGATTCGAA ATTGCAAAAA CTCGAACCCA TTATGGGGCA TGTTTCGATC
    TTAACTCAAT TAATAGTTGC TCAAAATCCA CAAAATTCTA AAGAAGAAAT AATCATTACT
    TCAGATAAAG ACGAGCATAT TCGTATTTCC CGATTTCCCA ATGCATTTGT CATTGAAGGG
    TTTTGCTTAG GTCATGAAGA TTTTGTATCT AGGATGTCCT TATATGACAA TCGTACTCTA
    ATATCTGGCG GTGGAGATAA TCACGTTTTC GTATGGGATT TAGAGAATTT CAAATGTTTG
    GATGCATTCG ATCTACGATC TGCCTTTTCC ACTTATCTCT CATTAAATCA ACCAATGGTG
    GTTTCAGTTA TTCTTCCTAT TTTTAAGCGC CAGCTTGTTG CTTTTGCGTG TGAAGGAATG
    GCGGGTCTTA TCTTCGCGAA GGTGACACCT GAAAAGAGAC TGTTGTTTCA TTCTGCTTTG
    AAGCTGTCAG GGCCTGTTCT TGACGCTGTT TTGCTTGACA CGGATACTGA TCAAATTCTC
    ATTTCTTTGG ACTCTAGTTT TACATTTGGT GCATGTTTTG AATGCGTGAA ATTTGATGAA
    GGGAATGCGG CTGTTCTTAC GAAACCCGAT GTAATAAAGC GTATAGAATC TGATGGTTTA
    ATATCAACCG AAAAGCCGTT CTGCCCGCTC GCTCAAATTC ACACATTACG CAAGAACCAC
    TCTAAATTCA TTCGCAGTGT TGAAACCGGT ACATCTTCGC CATCCGTTGA ATCTAAAGAT
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_588392.1
    CDS368..1633
    Translation

    Target ORF information:

    RefSeq Version NM_001023383.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe tRNA (guanine-N7-)-methyltransferase subunit Trm82 (SPCC18.13), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001023383.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGTGAAC AGCGAGTTTT TAAGCACCCT TGTCAATTTT TAACATGGAA TTCTAAACAC 
    AATTACATCG TGTGCTGTTC AGGTCCATAT CTATTGGGGT TCTCATGCTC TACAGGGGAA
    AAAATTTTTG AACATTGTTA TAGAGACAAT ATAAATGAAA AACATAGAGA GGCAGCTGCC
    TATGGAGAAG CGATTCGTCA AGTCGCATTT TCTAAAGACT ATTCTCGTAT GGCAACAGTT
    AGTGAAGACA AATGTTTGCG CCTTTGGGAT AGCACTCAGC CTGATAAAAT TGAACTTCTT
    TATCAAAAAA ACATACCAAA GCGATGTGCT GACTTGTGCT TTGCGGGCTC AAACGAAATT
    GTTTTTGGTG ATAAGTTTGG TGATGTCTAT TGTGTAGACG AAAATTGGTT TACTACTTCT
    GAAGTTACCG AAGAAAAAAA GTCAAATGTG GTTGAAGGAA AACAAGAACC TGTTAATAAT
    GACACTTTAA AGGATTCGAA ATTGCAAAAA CTCGAACCCA TTATGGGGCA TGTTTCGATC
    TTAACTCAAT TAATAGTTGC TCAAAATCCA CAAAATTCTA AAGAAGAAAT AATCATTACT
    TCAGATAAAG ACGAGCATAT TCGTATTTCC CGATTTCCCA ATGCATTTGT CATTGAAGGG
    TTTTGCTTAG GTCATGAAGA TTTTGTATCT AGGATGTCCT TATATGACAA TCGTACTCTA
    ATATCTGGCG GTGGAGATAA TCACGTTTTC GTATGGGATT TAGAGAATTT CAAATGTTTG
    GATGCATTCG ATCTACGATC TGCCTTTTCC ACTTATCTCT CATTAAATCA ACCAATGGTG
    GTTTCAGTTA TTCTTCCTAT TTTTAAGCGC CAGCTTGTTG CTTTTGCGTG TGAAGGAATG
    GCGGGTCTTA TCTTCGCGAA GGTGACACCT GAAAAGAGAC TGTTGTTTCA TTCTGCTTTG
    AAGCTGTCAG GGCCTGTTCT TGACGCTGTT TTGCTTGACA CGGATACTGA TCAAATTCTC
    ATTTCTTTGG ACTCTAGTTT TACATTTGGT GCATGTTTTG AATGCGTGAA ATTTGATGAA
    GGGAATGCGG CTGTTCTTAC GAAACCCGAT GTAATAAAGC GTATAGAATC TGATGGTTTA
    ATATCAACCG AAAAGCCGTT CTGCCCGCTC GCTCAAATTC ACACATTACG CAAGAACCAC
    TCTAAATTCA TTCGCAGTGT TGAAACCGGT ACATCTTCGC CATCCGTTGA ATCTAAAGAT
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L