wee1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following wee1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wee1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99770 NM_001022924.2
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Schizosaccharomyces pombe protein kinase Wee1 (wee1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99770
    Clone ID Related Accession (Same CDS sequence) NM_001022924.2
    Accession Version NM_001022924.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product protein kinase Wee1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022924.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGCTCTT CTTCTAATAC CAGCAGTCAT AGATCTTATG GCTTACGGCG GTCCCAACGC 
    TCCATGAATC TAAATCGTGC TACTTTGCTC GCTCCTCCAA CTCCATCGAG CCTGTATGAT
    GCTAACAATT CTACTTCATC TACCTCTTCG CAAAAGCCAA ATACCTCTTT CACTAGCTTA
    TTCGGTCCTC GCAAACAGAC CACTTCCTCC CCTTCCTTCT CTCATGCCGC CCCTCTTCAC
    CCTCTTTCTC CTCCTTCTTT TACACATTCA CAACCCCAGA TACAGGCACA ACCTGTACCT
    CGGCGGCCTT CCCTTTTTGA TAGACCCAAT CTGGTATCAC GCTCTTCCTC TCGGTTAGGT
    GATTCTCCAT CTCTTTCTCC TGTTGCCCAG GTGGCTAATC CCATCCACCA TACTGCCCCT
    TCACCCTCAG ACGTTCGTGC GTTTCCCATT CATAAAAATG CATCTACTGG TGTAAAACGA
    AGTTTCTTTT CTTCCTCCAT GTCAAACGGT GCAATGTCCC CTCCTTCCCA CTCTCCATCT
    CCCTTCCTTC AAAGCTCTCA ACACATACCT CCTTCTACTC CTGCTCAGAA GCTGCGAAAG
    AAAAACAATT TTGATTCTTT TCGTATCTCA AATTCCCACA TCTCTCCATT TGCATCGGGC
    TCCTTCTCTC CTTTTGCAAC TTCATCGCCT AATTTTTTAT CGACTTCCAC ACCCGCTCCT
    CCTAATTCAA ATAATGCTAA TCCTTCTACC CTTTTTTCGT CCATACCATC CTCTCGACAC
    ACTACCTCTA ATCATTTCCC TTCTAACTCC GCTCAATCCT CTTTGTTTTC TCCTACCGCT
    CGTCCACTTA CTGCACGCAA GCTTGGGTTT GCCTCCAGCC AAACTAAGTC TGCCGTATCT
    AACAACCATT CACGAAATAG CTCTAAGGAT GCATCATTTA TGATGAAAAG TTTCATCCCT
    TCTAATCGCT CACATCCTCA AACCCAACAA AATGAATCTT CCCTCTTTTC TGATAACAGT
    ATGGTTAACT CCTCTTCTAA TTCATTCTCC CTTTTCCCCA ATGCTACTCT ACCTAACCCA
    CCCTCATCTG AGTTGTTAAC TACTCCCTTT CAACAAATCA AACCTCCTTC CCAAGTTTTT
    ATGAGTACAG GTCTTTTATC CAAGCAACAT CGTCCGCGAA AGAATATTAA CTTTACACCT
    CTCCCTCCTA GTACTCCTTC AAAACCCTCT ACCTTTGTTC GTCCACATTC TTCCTCCACA
    GATTCGCCTC CATCACCCTC GACTCCTTCC AATACACAAA CCGACTCTTA TTTTATTCAA
    CGGGAAAACA CTCCTACAAA TCACAATTCC ATTCCTACTA TTCAGTTGGA GAAAAGTTCC
    ATGGACTTTT TGAGGTTCGA TCCTCCTCCT TCTGCTGTAA AAACTTCTCA CAACTATGGA
    TTACCATTTC TATCTAATCA GAGGTGTCCC GCTACGCCTA CTCGTAACCC ATTTGCGTTT
    GAAAATACTG TTTCCATTCA CATGGACGGT CGGCAACCTT CGCCTATCAA ATCGAGAAAT
    AATAATCAAA TGTCATTTGC CATGGAGGAA GAAGCTGATG TATCTCAACC CTCTTCTTCA
    AGCTTTACAT TATCCTTTCC TTCTGCTCTC ACCTCGTCCA AAGTATCATC ATCTACTTCA
    CATTTGCTTA CACGGTTTAG AAATGTTACT TTACTAGGAA GTGGAGAGTT TAGTGAGGTT
    TTTCAAGTTG AAGACCCTGT GGAAAAAACT TTAAAGTATG CAGTGAAAAA GCTCAAGGTA
    AAGTTTAGTG GACCAAAAGA ACGTAATCGA CTACTTCAGG AAGTCTCTAT TCAACGAGCC
    CTTAAAGGCC ATGATCACAT TGTAGAGCTT ATGGACTCAT GGGAACATGG GGGGTTTCTT
    TATATGCAAG TCGAATTGTG TGAAAATGGT AGTTTGGATC GTTTTCTTGA AGAGCAAGGA
    CAACTTTCAA GGCTCGATGA ATTTAGGGTA TGGAAAATAT TAGTAGAAGT AGCTCTTGGG
    CTACAGTTTA TTCATCATAA AAATTATGTT CATTTGGATT TAAAGCCTGC TAATGTTATG
    ATAACATTCG AGGGTACCTT GAAGATTGGT GATTTTGGAA TGGCCAGTGT TTGGCCTGTT
    CCGAGAGGCA TGGAAAGAGA AGGTGATTGT GAATACATTG CGCCTGAGGT TCTTGCTAAC
    CATTTGTATG ATAAACCTGC TGATATTTTC AGTTTGGGTA TCACAGTCTT CGAAGCTGCT
    GCTAACATTG TTCTTCCTGA TAACGGTCAG TCTTGGCAGA AATTACGTTC CGGTGACTTA
    TCAGATGCTC CTCGACTTTC TTCAACAGAC AACGGTTCAT CTCTTACTTC TTCTTCTCGT
    GAAACACCGG CTAATAGTAT AATAGGCCAA GGGGGACTTG ATCGCGTTGT AGAGTGGATG
    CTATCTCCAG AGCCTAGGAA CCGACCTACC ATAGATCAAA TTTTGGCAAC TGATGAGGTT
    TGCTGGGTTG AAATGCGTAG AAAAGCCGGA GCCATAATTT ATGAAGGTAT TCATGGATCT
    TCTTCTAACC CCCAGGGTGA TCAAATGATG GAAGATTGGC AGGTGAATGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_587933.1
    CDS447..3080
    Translation

    Target ORF information:

    RefSeq Version NM_001022924.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe protein kinase Wee1 (wee1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGCTCTT CTTCTAATAC CAGCAGTCAT AGATCTTATG GCTTACGGCG GTCCCAACGC 
    TCCATGAATC TAAATCGTGC TACTTTGCTC GCTCCTCCAA CTCCATCGAG CCTGTATGAT
    GCTAACAATT CTACTTCATC TACCTCTTCG CAAAAGCCAA ATACCTCTTT CACTAGCTTA
    TTCGGTCCTC GCAAACAGAC CACTTCCTCC CCTTCCTTCT CTCATGCCGC CCCTCTTCAC
    CCTCTTTCTC CTCCTTCTTT TACACATTCA CAACCCCAGA TACAGGCACA ACCTGTACCT
    CGGCGGCCTT CCCTTTTTGA TAGACCCAAT CTGGTATCAC GCTCTTCCTC TCGGTTAGGT
    GATTCTCCAT CTCTTTCTCC TGTTGCCCAG GTGGCTAATC CCATCCACCA TACTGCCCCT
    TCACCCTCAG ACGTTCGTGC GTTTCCCATT CATAAAAATG CATCTACTGG TGTAAAACGA
    AGTTTCTTTT CTTCCTCCAT GTCAAACGGT GCAATGTCCC CTCCTTCCCA CTCTCCATCT
    CCCTTCCTTC AAAGCTCTCA ACACATACCT CCTTCTACTC CTGCTCAGAA GCTGCGAAAG
    AAAAACAATT TTGATTCTTT TCGTATCTCA AATTCCCACA TCTCTCCATT TGCATCGGGC
    TCCTTCTCTC CTTTTGCAAC TTCATCGCCT AATTTTTTAT CGACTTCCAC ACCCGCTCCT
    CCTAATTCAA ATAATGCTAA TCCTTCTACC CTTTTTTCGT CCATACCATC CTCTCGACAC
    ACTACCTCTA ATCATTTCCC TTCTAACTCC GCTCAATCCT CTTTGTTTTC TCCTACCGCT
    CGTCCACTTA CTGCACGCAA GCTTGGGTTT GCCTCCAGCC AAACTAAGTC TGCCGTATCT
    AACAACCATT CACGAAATAG CTCTAAGGAT GCATCATTTA TGATGAAAAG TTTCATCCCT
    TCTAATCGCT CACATCCTCA AACCCAACAA AATGAATCTT CCCTCTTTTC TGATAACAGT
    ATGGTTAACT CCTCTTCTAA TTCATTCTCC CTTTTCCCCA ATGCTACTCT ACCTAACCCA
    CCCTCATCTG AGTTGTTAAC TACTCCCTTT CAACAAATCA AACCTCCTTC CCAAGTTTTT
    ATGAGTACAG GTCTTTTATC CAAGCAACAT CGTCCGCGAA AGAATATTAA CTTTACACCT
    CTCCCTCCTA GTACTCCTTC AAAACCCTCT ACCTTTGTTC GTCCACATTC TTCCTCCACA
    GATTCGCCTC CATCACCCTC GACTCCTTCC AATACACAAA CCGACTCTTA TTTTATTCAA
    CGGGAAAACA CTCCTACAAA TCACAATTCC ATTCCTACTA TTCAGTTGGA GAAAAGTTCC
    ATGGACTTTT TGAGGTTCGA TCCTCCTCCT TCTGCTGTAA AAACTTCTCA CAACTATGGA
    TTACCATTTC TATCTAATCA GAGGTGTCCC GCTACGCCTA CTCGTAACCC ATTTGCGTTT
    GAAAATACTG TTTCCATTCA CATGGACGGT CGGCAACCTT CGCCTATCAA ATCGAGAAAT
    AATAATCAAA TGTCATTTGC CATGGAGGAA GAAGCTGATG TATCTCAACC CTCTTCTTCA
    AGCTTTACAT TATCCTTTCC TTCTGCTCTC ACCTCGTCCA AAGTATCATC ATCTACTTCA
    CATTTGCTTA CACGGTTTAG AAATGTTACT TTACTAGGAA GTGGAGAGTT TAGTGAGGTT
    TTTCAAGTTG AAGACCCTGT GGAAAAAACT TTAAAGTATG CAGTGAAAAA GCTCAAGGTA
    AAGTTTAGTG GACCAAAAGA ACGTAATCGA CTACTTCAGG AAGTCTCTAT TCAACGAGCC
    CTTAAAGGCC ATGATCACAT TGTAGAGCTT ATGGACTCAT GGGAACATGG GGGGTTTCTT
    TATATGCAAG TCGAATTGTG TGAAAATGGT AGTTTGGATC GTTTTCTTGA AGAGCAAGGA
    CAACTTTCAA GGCTCGATGA ATTTAGGGTA TGGAAAATAT TAGTAGAAGT AGCTCTTGGG
    CTACAGTTTA TTCATCATAA AAATTATGTT CATTTGGATT TAAAGCCTGC TAATGTTATG
    ATAACATTCG AGGGTACCTT GAAGATTGGT GATTTTGGAA TGGCCAGTGT TTGGCCTGTT
    CCGAGAGGCA TGGAAAGAGA AGGTGATTGT GAATACATTG CGCCTGAGGT TCTTGCTAAC
    CATTTGTATG ATAAACCTGC TGATATTTTC AGTTTGGGTA TCACAGTCTT CGAAGCTGCT
    GCTAACATTG TTCTTCCTGA TAACGGTCAG TCTTGGCAGA AATTACGTTC CGGTGACTTA
    TCAGATGCTC CTCGACTTTC TTCAACAGAC AACGGTTCAT CTCTTACTTC TTCTTCTCGT
    GAAACACCGG CTAATAGTAT AATAGGCCAA GGGGGACTTG ATCGCGTTGT AGAGTGGATG
    CTATCTCCAG AGCCTAGGAA CCGACCTACC ATAGATCAAA TTTTGGCAAC TGATGAGGTT
    TGCTGGGTTG AAATGCGTAG AAAAGCCGGA GCCATAATTT ATGAAGGTAT TCATGGATCT
    TCTTCTAACC CCCAGGGTGA TCAAATGATG GAAGATTGGC AGGTGAATGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L