Gene Symbol | wee1 |
Entrez Gene ID | 2539123 |
Full Name | M phase inhibitor protein kinase Wee1 |
Gene Type | protein-coding |
Organism | Schizosaccharomyces pombe(fission yeast) |
ORF » Species Summary » Schizosaccharomyces pombe » wee1 cDNA ORF clone
Gene Symbol | wee1 |
Entrez Gene ID | 2539123 |
Full Name | M phase inhibitor protein kinase Wee1 |
Gene Type | protein-coding |
Organism | Schizosaccharomyces pombe(fission yeast) |
mRNA | Protein | Name |
---|---|---|
NM_001022924.2 | NP_587933.1 | protein kinase Wee1 |
The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
This cell cycle arrest is dependent on the Wee1 tyrosine kinase inhibiting the key cell cycle regulator, CDK1/Cdc2. Mutant cells lacking Wee1 are not arrested at G2 upon glucose limitation and lose viability faster than the wild-type cells under glucose-depleted quiescent conditions, suggesting that this cell cycle arrest is required for extension of chronological lifespan
Title: Glucose restriction induces transient G2 cell cycle arrest extending cellular chronological lifespan.
in fission yeast Wee1 is the major inhibitory kinase regulating CDK activity in S phase.
Title: Consequences of abnormal CDK activity in S phase.
Wee1 phosphorylates Cdc2 Tyr15 which leads to replication slowdown in the fission yeast under nitrosative stress.
Title: Nitrosative stress induces a novel intra-S checkpoint pathway in Schizosaccharomyces pombe involving phosphorylation of Cdc2 by Wee1.
Substitution of a conserved methionine residue to phenylalanine in Plo1, Orb5 and Wee1 kinases increased the sensitivities to ATP analogues.
Title: Mutation of a conserved residue enhances the sensitivity of analogue-sensitised kinases to generate a novel approach to the study of mitosis in fission yeast.
Dnt1 regulates G2/M transition and cytokinesis by downregulating Wee1 kinase.
Title: Fission yeast nucleolar protein Dnt1 regulates G2/M transition and cytokinesis by downregulating Wee1 kinase.
The following wee1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wee1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSc99770 | |
Clone ID Related Accession (Same CDS sequence) | NM_001022924.2 | |
Accession Version | NM_001022924.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-04-02 | |
Organism | Schizosaccharomyces pombe(fission yeast) | |
Product | protein kinase Wee1 | |
Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022924.1. |
1 | ATGAGCTCTT CTTCTAATAC CAGCAGTCAT AGATCTTATG GCTTACGGCG GTCCCAACGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_587933.1 |
CDS | 447..3080 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001022924.2 |
1 | ATGAGCTCTT CTTCTAATAC CAGCAGTCAT AGATCTTATG GCTTACGGCG GTCCCAACGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The genome sequence of Schizosaccharomyces pombe. |