SPCC1223.01 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPCC1223.01 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPCC1223.01 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99106 NM_001023337.2
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Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPCC1223.01), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99106
    Clone ID Related Accession (Same CDS sequence) NM_001023337.2
    Accession Version NM_001023337.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative ubiquitin-protein ligase E3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023337.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGCCTT CGGGCCCCAA TTTAAATAAT AAGGAGCACA ACAGAGCATC GGAAAAAAAG 
    AATTCTAGGA CTCATAATAA AAAAACTAAT AGGAATCAAA GTAAAGAGAA ACCGGTAAGC
    TCTAGGTCGG TAGAAACTCC AAAAAATGCA GTTTGCTTAG AGCCGGTTGG CACAGATCCT
    GTCAGTAATG TCGCTACTGT TGATGCTTCC AAAGAAGAAC AGGATGAGGA TGAGCAAATT
    TGCTTCATTT GTGCTGAAGG TATTACTTAC AGCTGCGTCC TTCCTTGCAA TCATCGAATG
    TGTCATGTTT GTGCTTTGAG ATTGCGAGCG CTATACAAAA CGAAGGAATG TACGTTTTGT
    AAAACCGAAT GGGATACGGT TCTCATTACT AAAGATCATG AAATCGATAT TCATGATGTC
    GATTTAGCAA AACTTCCTTT CCAAGATGAG AAACTTGGAA TTGTGTATTC TGACGAGCAT
    GCTCAAGAAG AATCCAACCT CTTGCTTCAA TTCAACTGTC CAGAAGACGC TTGCGATATC
    ACATGCAAAG GCTGGTTTGA TTTAAAGCTG CATGCGAAGG TCAAACACCA TAAGTTTTTC
    TGTGATCTTT GTGTTAAGAA CAAAAAGGTT TTTACCCATG AACACACGCT ATTTTCCAAG
    AAAGGATTGA CAAAGCACAA TGAAGTTGGA GATCAGGGAT CCGATTTAGA GATTACTGGA
    TTCAAGGGTC ACCCGAAGTG TGAGTTTTGT AATACCCATT TTTATGATGA TGATGAGTTG
    TTTAAGCATT GCCGTGAAAA ACATGAACGA TGTTACATCT GTGACCAAGT TGCAGGAAGG
    CCGACGCATC AGTACTTTAA AAATTATGAT TCGTTAGAGC GCCACTTTGA AAAAGATCAT
    TATATTTGTC GTGAAAGGGA GTGTTTGGAA CGTAAATTTG TTGTTTTTGG AACTGAAATA
    GATTTGAAAG CGCATCAGTT GGATGAGCAT CCCCACAACT TTACTCAACG CGAACTTCGA
    GAAGCACGAA GAATTATTCC TCAATTTAGT TACGACCCAC CCGGCGCGTC CGGCAGGAAC
    CGTCGTGAAC GAACTTCTTC CACACCATCC GAACAATCTA CTTCAGTGAA TGAGACTGCC
    AACTCACTCT CTAACCTACA CCTTTCCCGT GGAGAGATTG CTCACTTACG ACAAGAAGAA
    TATGTTCGCG AGCAACAAGC TCGCCATCGT GATTTTGGTT TCACGTTATC AAATCCGGCG
    CCTACATCTG CCCGCCCAGC GACATCTACT AGAACCATCA GCAGAGGAAA AACTAGAACG
    TTAAGAAACG AAGATTTCCC CTCTTTAGCC GAGGTTGCTA ATCAAAATAG CTCTTCCGCA
    CCCTCAGTAC CTGTCTCCGC GCCTCGTCTG TCTGGTAAGT CTGCTTCAAG AAATCATGTT
    CCATCTCCAC CCAAGGGCAC TAAATCCCCG ATGGCCTCTT CCGAGCAAGC ACAACACCAA
    CAAGTAATTG ATCGGATGCA AAAATTGACG AATTACGATG ATCACAAAAT TAATGACTTT
    AAATTTGCTG TTTCTTCCTT CCGTGGTAAT GTTATGCCCG CTCGTGAAGC TGTTGCTCGC
    ATTACAAAAT TAGTTGCAAA GCCTCATGAG CAATTGTCGG GCGTGTTCAA CCAAATAGCA
    AATCTTCTAG AAAATAAAGA GAAGTCAAGG GAATTGTTGG AAGCTTGGCA GGAATGGAAA
    ATACTGAATG CGAAAGATGA TACTCGTATA GGTACTACTA ACTCTAATCT TTTGCGCTTG
    AAGAGATCAA ATAGGACTGC TGCTCAAACA GCTAGTGTTT GGAATCGTAT TGAGCGTGCA
    GCAGCTCACG ATGGGCCTTC TTTGTCTGCT CCATCATCTT CTATAAACCT AGCAAATATT
    ACCTCAAGGC CAACGAACAG CTCAGCTGCA AATACTCCTT CTTGGGGTGT GCGCAAAGCA
    CGTGCATCTG CGTTAAATGC AAGATCTGAA GAAGATTTTC CCGCACTTCC TCCTTCTACC
    TCGAAGAGAA TTTCTGTTCA ACTCGGAAAA AAGCAGGCTC GTCCCGTTGA TTCATGGGGG
    TCTACACCAA ATACTTCTTC CAATCGTAAT AGTAATACGA TGGGTGTTTC AAAGAAAAAA
    AACGGAAAAA AGCAAACTGT TTTGTTTCAC ATTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_588346.2
    CDS15..2213
    Translation

    Target ORF information:

    RefSeq Version NM_001023337.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPCC1223.01), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001023337.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGCCTT CGGGCCCCAA TTTAAATAAT AAGGAGCACA ACAGAGCATC GGAAAAAAAG 
    AATTCTAGGA CTCATAATAA AAAAACTAAT AGGAATCAAA GTAAAGAGAA ACCGGTAAGC
    TCTAGGTCGG TAGAAACTCC AAAAAATGCA GTTTGCTTAG AGCCGGTTGG CACAGATCCT
    GTCAGTAATG TCGCTACTGT TGATGCTTCC AAAGAAGAAC AGGATGAGGA TGAGCAAATT
    TGCTTCATTT GTGCTGAAGG TATTACTTAC AGCTGCGTCC TTCCTTGCAA TCATCGAATG
    TGTCATGTTT GTGCTTTGAG ATTGCGAGCG CTATACAAAA CGAAGGAATG TACGTTTTGT
    AAAACCGAAT GGGATACGGT TCTCATTACT AAAGATCATG AAATCGATAT TCATGATGTC
    GATTTAGCAA AACTTCCTTT CCAAGATGAG AAACTTGGAA TTGTGTATTC TGACGAGCAT
    GCTCAAGAAG AATCCAACCT CTTGCTTCAA TTCAACTGTC CAGAAGACGC TTGCGATATC
    ACATGCAAAG GCTGGTTTGA TTTAAAGCTG CATGCGAAGG TCAAACACCA TAAGTTTTTC
    TGTGATCTTT GTGTTAAGAA CAAAAAGGTT TTTACCCATG AACACACGCT ATTTTCCAAG
    AAAGGATTGA CAAAGCACAA TGAAGTTGGA GATCAGGGAT CCGATTTAGA GATTACTGGA
    TTCAAGGGTC ACCCGAAGTG TGAGTTTTGT AATACCCATT TTTATGATGA TGATGAGTTG
    TTTAAGCATT GCCGTGAAAA ACATGAACGA TGTTACATCT GTGACCAAGT TGCAGGAAGG
    CCGACGCATC AGTACTTTAA AAATTATGAT TCGTTAGAGC GCCACTTTGA AAAAGATCAT
    TATATTTGTC GTGAAAGGGA GTGTTTGGAA CGTAAATTTG TTGTTTTTGG AACTGAAATA
    GATTTGAAAG CGCATCAGTT GGATGAGCAT CCCCACAACT TTACTCAACG CGAACTTCGA
    GAAGCACGAA GAATTATTCC TCAATTTAGT TACGACCCAC CCGGCGCGTC CGGCAGGAAC
    CGTCGTGAAC GAACTTCTTC CACACCATCC GAACAATCTA CTTCAGTGAA TGAGACTGCC
    AACTCACTCT CTAACCTACA CCTTTCCCGT GGAGAGATTG CTCACTTACG ACAAGAAGAA
    TATGTTCGCG AGCAACAAGC TCGCCATCGT GATTTTGGTT TCACGTTATC AAATCCGGCG
    CCTACATCTG CCCGCCCAGC GACATCTACT AGAACCATCA GCAGAGGAAA AACTAGAACG
    TTAAGAAACG AAGATTTCCC CTCTTTAGCC GAGGTTGCTA ATCAAAATAG CTCTTCCGCA
    CCCTCAGTAC CTGTCTCCGC GCCTCGTCTG TCTGGTAAGT CTGCTTCAAG AAATCATGTT
    CCATCTCCAC CCAAGGGCAC TAAATCCCCG ATGGCCTCTT CCGAGCAAGC ACAACACCAA
    CAAGTAATTG ATCGGATGCA AAAATTGACG AATTACGATG ATCACAAAAT TAATGACTTT
    AAATTTGCTG TTTCTTCCTT CCGTGGTAAT GTTATGCCCG CTCGTGAAGC TGTTGCTCGC
    ATTACAAAAT TAGTTGCAAA GCCTCATGAG CAATTGTCGG GCGTGTTCAA CCAAATAGCA
    AATCTTCTAG AAAATAAAGA GAAGTCAAGG GAATTGTTGG AAGCTTGGCA GGAATGGAAA
    ATACTGAATG CGAAAGATGA TACTCGTATA GGTACTACTA ACTCTAATCT TTTGCGCTTG
    AAGAGATCAA ATAGGACTGC TGCTCAAACA GCTAGTGTTT GGAATCGTAT TGAGCGTGCA
    GCAGCTCACG ATGGGCCTTC TTTGTCTGCT CCATCATCTT CTATAAACCT AGCAAATATT
    ACCTCAAGGC CAACGAACAG CTCAGCTGCA AATACTCCTT CTTGGGGTGT GCGCAAAGCA
    CGTGCATCTG CGTTAAATGC AAGATCTGAA GAAGATTTTC CCGCACTTCC TCCTTCTACC
    TCGAAGAGAA TTTCTGTTCA ACTCGGAAAA AAGCAGGCTC GTCCCGTTGA TTCATGGGGG
    TCTACACCAA ATACTTCTTC CAATCGTAAT AGTAATACGA TGGGTGTTTC AAAGAAAAAA
    AACGGAAAAA AGCAAACTGT TTTGTTTCAC ATTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L