robo2 cDNA ORF clone, Poecilia mexicana

Gene
Clones
gRNAs

Summary

Gene Symbol	robo2
Entrez Gene ID	106905574
Full Name	roundabout guidance receptor 2
Gene Type	protein-coding
Organism	Poecilia mexicana

mRNA and Protein(s)

mRNA	Protein	Name
XM_014970471.1	XP_014825957.1	roundabout homolog 2 isoform X2
XM_014970470.1	XP_014825956.1	roundabout homolog 2 isoform X1

Pathways from BioSystems
Gene Ontology

The following robo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo90782	XM_014970470.1 Latest version!	Poecilia mexicana roundabout guidance receptor 2 (robo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo90784	XM_014970471.1 Latest version!	Poecilia mexicana roundabout guidance receptor 2 (robo2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

robo2 ( XM_014970470.1 ) cDNA ORF clone, Poecilia mexicana -> XP_014825956.1 Poecilia mexicana roundabout guidance receptor 2 (robo2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo90782
Clone ID Related Accession (Same CDS sequence)	XM_014970470.1
Accession Version	XM_014970470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia mexicana
Product	roundabout homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015096672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia mexicana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTG TTGCGCTGCT GAAATGTCAG GCATCTGGAG ACCCCATCCC CTCCGTCAGC 
TGGCTGAAGG ACGGGGTCAG TCTACTGGGA AAGGACTCCC GCATGTCGCT ACAGGAGCTG 
GGCAGCCTGC AAATCAAAAA CATCAAGCTC TCAGACTCGG GGATCTACAC GTGTGTGGCC 
ACCAGCTCAA GCGGGGAAAC GTCGTGGAGC GCTTTCTTGG AAGTCAAAGA ATCATTGGCA 
GTGATTGTCA CAAAGAGCTA TGATGAGATC GAGCTTCCAG GTCCACCGTC TAAGCCCCAG 
GTCACCGACG TTACCAAGAA CAGCGTGTCG TTGTCGTGGC AGCCCGGGAT GACCGGAGCC 
TCTCCTGTCT CTTCCTTTGT CATTGAAGCT TTCAGTCAGT CTGTGAGCAA CAGCTGGCAA 
ACCGTGGCGG ACCATGTCAA GACCACCCAG TTCACCGTCA AAGGTCTCCG TCCCAACACC 
ATCTACCTGT TCATGGTCCG AGCCGTCAAC GGCCAAGGCT TAAGTGATCC GAGCCCAATG 
TCTGAACCTG TTAGAACTCA AGATATAAGC CCTCCAGCCC AGGGAGTGGA CCACAGGCAT 
GTGCAGAAAG AGCTCGGAGA GGTCATAGTT CGCCTGCATA ATCCTGTTGT CCTGAGTCCC 
ACCACCATTC AGGTCACATG GACAGTGGAC CGCCAGTCCC AGTTCATCCA GGGTTACCGG 
GTTCTATACA GGCAGACGTC TGGGCTTCCT TCGCCAGGGG CGTGGCAGAC CCAGGACGTG 
AAGGTGCCCT CGGAACGCAG CGTCGTCCTG TCATCGCTGA AGAAGGGCAT CGTCTATGAG 
ATCAAGGTCC GGCCGTACTT CAACGAGTTT CAAGGCATGG ACAGCGAATC GAAGACGGCG 
CGGACCACTG AGGAGGCTCC CAGCGCCCCC CCTCAGCAGG TAACCGTCCT GACAGTGGGA 
AACCAGAACA GCACCTCCAT CAGCATCTCC TGGGACCCCC CTCCGCACGA CCACCAGAAC 
GGCATTATCC AGGAGTACAA GATCTGGTGT CTGGGGAACG AGACGCGTTT CCACGTGAAC 
AAGACGGTGG ACGCAGCCAT CCGCTCCGTG GTGGTGGGAG GGCTGCAGGT GGGCGTGGTG 
TATCACGTGG AGGTTGCCGC CAGCACCAGT GCAGGAGTGG GAGTGAAGAG TGAACCTCAG 
TCTATAATCA TCGGGAAAGA GTTCCGGGAC GTGATCATAC ACGGAAACCG GAACAACAGC 
ATCACGGAGC AGATAACGGA CGTGGTGAAG CAGCCGGCCT TCATCGCTGG CATTGGAGGC 
GCCTGCTGGG TCATCCTGAT GGGCTTCAGC ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA 
AAGGGTCTGA GCAACTATGC AGTTCAGTCC TTCACCTTCA CCCCTGCAGT GACGTTCCAA 
AGAGTCGACG GCGGTCTGAT GAGCAATGGA AGCCGTCCAG GCCTGTTGAA CGCCAGTGAC 
CCGGGCTACC CCTGGTTAGC CGACTCCTGG CCAGCGACCA GCCTGCCCGT CAACAGCAGC 
TCCGGAGGAC CAAACGACTT GACTAAATTT GGTCGAGGAG ATCTCCCCAC TGGACAGGGA 
GATAAAACAG GCACCATGCT GTCTGACGGA GCCATCTACA GCAGCATAGA CTTTACCACC 
AAGGCTAACT ACAACAGTCC TGGTCAGACA TCCCAAGCCA ATCCTTATGC CACCACTCAG 
ATCCTCCACT CCAGCAGCAT CCATGAGCTG GCTGTGGACC TGCCTGACGC CCAGTGGAAG 
GCCTCACTTC AGACTAAGCA AGAAATGGCA AACCTGGGCT ACTCCCTGCC TGACAAGAAC 
TCCTGCAACA ACAGTGGAAA AGGAGGGAAG AAGAAGAAAA CCAAGGCTGG ATCTAAACCT 
CCCAAAGCTA ATGGCTCCAA CTGGGCCAAT GTTCCCCTGC CCCCTCCCCC GATTCACCCT 
CTACCCGGCA CAGAGATGGA CCTGTATCCC AATGAGCACC ATGACGGAGG AGGGTATGAG 
AATGACACCT GGGGCCCACC CATGCCTGTG CAGACGTACC TTCATCAAGG CATGGAAGAT 
GAGCTGGAAG AAGAGGAGGA AAGGGTTCCC ACGCCACCCA TCCGAGGAGT GGCCTCCTCA 
CCGGCTGCCG TCTCTTTTGG ACAGCAGTCC ACAGCCACCC TCACCCCATC TCCCCGGGAT 
GAGATGCAGC CCATGCTCCA GGCCCATCTG GATGAGCTGA CCAGAGCATA CCAACTGGAC 
ATGGCTAAAC AGACATGGCA CATGCAGGGA GGCTCTCGTC CTCCCCTGGC ACCAGCTCCG 
CCAGTGGGCT ATGTGTCGAG CACAATGGTT TCCGACCTGG AGACTGACCT GCAAGACGAG 
GAGGACGAAG AGGAGGACGA GGACGAGGAT GAGGGCTACG GAGCCAGACA TCCCCGTGGT 
ATCGAGCACA CACCAGGGTC TAGTATGGAC AACCTGGACA GCTCTATAAC AGGGTCCATG 
GTGAATGGGT GGGGCTCGGC CTCGGAGGAT GAGCGTAATT TTTCCAGCCA TAGATCCAGT 
CTGGGCAGTT CTTCCGATGG CTCCATCTTT ACCCACTGCA GCTTTGCCCA GGCCCTTGTG 
GCTGCTGCAG ACAAGGCAGG CTACCGCCTT GAGGGAACCA GCCTCAGCAA GAGAGGTAAA 
GGCTTGTCCC ACAGGCCCCG TCCCAGCAGT CCATTTTCAA CAGATGGCAG CACAGTCGGC 
ACACACAGCT TGGGCCACCA GAGGGCCACC AGGCCCACCC GCAAACCACG AAGTCAAGGC 
ACAACACCTC ACAGGAGAGA CGCTGGTGCA GACGACCTAC CTCCCCCCCC TGAACCTCCT 
CCCTGTCAGA TCCAGGGTCG CATTCAGGGG ACCCGCAGTG TAAACAGCAT GGCACCACCC 
ACGGACAGGA AGGCTTCGTC TCTGGAGCGC ACGCCCATGT CAGGGCCGCT CTGTGGGCCA 
GACGACACGA AGAGCTCAAT TGACAGACAG ATGCGAACCT CTCTGGAACA TCACCGCCAG 
GCCCAGGACC GACTGGGCTC CATGGACCGG ACTGACGATG CCCGCAGGTG CCACAAATCA 
GAGGATGGGT GTGTCCCGTA TAGCAAGCCA TCCTTCCCCT CCCCCGGGGG CCACAGTTCC 
TCGGGGACGG CGTCCTCCAA GGGGTCAACA GGGCCACGCA AGACCGACGG GCCGCGGCAG 
CCACAACAGT GGACCGCCAC GGAGCATGCC GAATTTCTGG GGGCCAATGG GCATGGAGAG 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014825956.1
CDS	144..3449
Translation	MDSVALLKCQASGDPIPSVSWLKDGVSLLGKDSRMSLQELGSLQIKNIKLSDSGIYTCVATSSSGETSWSAFLEVKESLAVIVTKSYDEIELPGPPSKPQVTDVTKNSVSLSWQPGMTGASPVSSFVIEAFSQSVSNSWQTVADHVKTTQFTVKGLRPNTIYLFMVRAVNGQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQTSGLPSPGAWQTQDVKVPSERSVVLSSLKKGIVYEIKVRPYFNEFQGMDSESKTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPHDHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQVGVVYHVEVAASTSAGVGVKSEPQSIIIGKEFRDVIIHGNRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDPGYPWLADSWPATSLPVNSSSGGPNDLTKFGRGDLPTGQGDKTGTMLSDGAIYSSIDFTTKANYNSPGQTSQANPYATTQILHSSSIHELAVDLPDAQWKASLQTKQEMANLGYSLPDKNSCNNSGKGGKKKKTKAGSKPPKANGSNWANVPLPPPPIHPLPGTEMDLYPNEHHDGGGYENDTWGPPMPVQTYLHQGMEDELEEEEERVPTPPIRGVASSPAAVSFGQQSTATLTPSPRDEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGSRPPLAPAPPVGYVSSTMVSDLETDLQDEEDEEEDEDEDEGYGARHPRGIEHTPGSSMDNLDSSITGSMVNGWGSASEDERNFSSHRSSLGSSSDGSIFTHCSFAQALVAAADKAGYRLEGTSLSKRGKGLSHRPRPSSPFSTDGSTVGTHSLGHQRATRPTRKPRSQGTTPHRRDAGADDLPPPPEPPPCQIQGRIQGTRSVNSMAPPTDRKASSLERTPMSGPLCGPDDTKSSIDRQMRTSLEHHRQAQDRLGSMDRTDDARRCHKSEDGCVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTDGPRQPQQWTATEHAEFLGANGHGEL

Target ORF information:

RefSeq Version	XM_014970470.1
Organism	Poecilia mexicana
Definition	Poecilia mexicana roundabout guidance receptor 2 (robo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014970470.1

ORF Insert Sequence:

ATGGACAGTG TTGCGCTGCT GAAATGTCAG GCATCTGGAG ACCCCATCCC CTCCGTCAGC 
TGGCTGAAGG ACGGGGTCAG TCTACTGGGA AAGGACTCCC GCATGTCGCT ACAGGAGCTG 
GGCAGCCTGC AAATCAAAAA CATCAAGCTC TCAGACTCGG GGATCTACAC GTGTGTGGCC 
ACCAGCTCAA GCGGGGAAAC GTCGTGGAGC GCTTTCTTGG AAGTCAAAGA ATCATTGGCA 
GTGATTGTCA CAAAGAGCTA TGATGAGATC GAGCTTCCAG GTCCACCGTC TAAGCCCCAG 
GTCACCGACG TTACCAAGAA CAGCGTGTCG TTGTCGTGGC AGCCCGGGAT GACCGGAGCC 
TCTCCTGTCT CTTCCTTTGT CATTGAAGCT TTCAGTCAGT CTGTGAGCAA CAGCTGGCAA 
ACCGTGGCGG ACCATGTCAA GACCACCCAG TTCACCGTCA AAGGTCTCCG TCCCAACACC 
ATCTACCTGT TCATGGTCCG AGCCGTCAAC GGCCAAGGCT TAAGTGATCC GAGCCCAATG 
TCTGAACCTG TTAGAACTCA AGATATAAGC CCTCCAGCCC AGGGAGTGGA CCACAGGCAT 
GTGCAGAAAG AGCTCGGAGA GGTCATAGTT CGCCTGCATA ATCCTGTTGT CCTGAGTCCC 
ACCACCATTC AGGTCACATG GACAGTGGAC CGCCAGTCCC AGTTCATCCA GGGTTACCGG 
GTTCTATACA GGCAGACGTC TGGGCTTCCT TCGCCAGGGG CGTGGCAGAC CCAGGACGTG 
AAGGTGCCCT CGGAACGCAG CGTCGTCCTG TCATCGCTGA AGAAGGGCAT CGTCTATGAG 
ATCAAGGTCC GGCCGTACTT CAACGAGTTT CAAGGCATGG ACAGCGAATC GAAGACGGCG 
CGGACCACTG AGGAGGCTCC CAGCGCCCCC CCTCAGCAGG TAACCGTCCT GACAGTGGGA 
AACCAGAACA GCACCTCCAT CAGCATCTCC TGGGACCCCC CTCCGCACGA CCACCAGAAC 
GGCATTATCC AGGAGTACAA GATCTGGTGT CTGGGGAACG AGACGCGTTT CCACGTGAAC 
AAGACGGTGG ACGCAGCCAT CCGCTCCGTG GTGGTGGGAG GGCTGCAGGT GGGCGTGGTG 
TATCACGTGG AGGTTGCCGC CAGCACCAGT GCAGGAGTGG GAGTGAAGAG TGAACCTCAG 
TCTATAATCA TCGGGAAAGA GTTCCGGGAC GTGATCATAC ACGGAAACCG GAACAACAGC 
ATCACGGAGC AGATAACGGA CGTGGTGAAG CAGCCGGCCT TCATCGCTGG CATTGGAGGC 
GCCTGCTGGG TCATCCTGAT GGGCTTCAGC ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA 
AAGGGTCTGA GCAACTATGC AGTTCAGTCC TTCACCTTCA CCCCTGCAGT GACGTTCCAA 
AGAGTCGACG GCGGTCTGAT GAGCAATGGA AGCCGTCCAG GCCTGTTGAA CGCCAGTGAC 
CCGGGCTACC CCTGGTTAGC CGACTCCTGG CCAGCGACCA GCCTGCCCGT CAACAGCAGC 
TCCGGAGGAC CAAACGACTT GACTAAATTT GGTCGAGGAG ATCTCCCCAC TGGACAGGGA 
GATAAAACAG GCACCATGCT GTCTGACGGA GCCATCTACA GCAGCATAGA CTTTACCACC 
AAGGCTAACT ACAACAGTCC TGGTCAGACA TCCCAAGCCA ATCCTTATGC CACCACTCAG 
ATCCTCCACT CCAGCAGCAT CCATGAGCTG GCTGTGGACC TGCCTGACGC CCAGTGGAAG 
GCCTCACTTC AGACTAAGCA AGAAATGGCA AACCTGGGCT ACTCCCTGCC TGACAAGAAC 
TCCTGCAACA ACAGTGGAAA AGGAGGGAAG AAGAAGAAAA CCAAGGCTGG ATCTAAACCT 
CCCAAAGCTA ATGGCTCCAA CTGGGCCAAT GTTCCCCTGC CCCCTCCCCC GATTCACCCT 
CTACCCGGCA CAGAGATGGA CCTGTATCCC AATGAGCACC ATGACGGAGG AGGGTATGAG 
AATGACACCT GGGGCCCACC CATGCCTGTG CAGACGTACC TTCATCAAGG CATGGAAGAT 
GAGCTGGAAG AAGAGGAGGA AAGGGTTCCC ACGCCACCCA TCCGAGGAGT GGCCTCCTCA 
CCGGCTGCCG TCTCTTTTGG ACAGCAGTCC ACAGCCACCC TCACCCCATC TCCCCGGGAT 
GAGATGCAGC CCATGCTCCA GGCCCATCTG GATGAGCTGA CCAGAGCATA CCAACTGGAC 
ATGGCTAAAC AGACATGGCA CATGCAGGGA GGCTCTCGTC CTCCCCTGGC ACCAGCTCCG 
CCAGTGGGCT ATGTGTCGAG CACAATGGTT TCCGACCTGG AGACTGACCT GCAAGACGAG 
GAGGACGAAG AGGAGGACGA GGACGAGGAT GAGGGCTACG GAGCCAGACA TCCCCGTGGT 
ATCGAGCACA CACCAGGGTC TAGTATGGAC AACCTGGACA GCTCTATAAC AGGGTCCATG 
GTGAATGGGT GGGGCTCGGC CTCGGAGGAT GAGCGTAATT TTTCCAGCCA TAGATCCAGT 
CTGGGCAGTT CTTCCGATGG CTCCATCTTT ACCCACTGCA GCTTTGCCCA GGCCCTTGTG 
GCTGCTGCAG ACAAGGCAGG CTACCGCCTT GAGGGAACCA GCCTCAGCAA GAGAGGTAAA 
GGCTTGTCCC ACAGGCCCCG TCCCAGCAGT CCATTTTCAA CAGATGGCAG CACAGTCGGC 
ACACACAGCT TGGGCCACCA GAGGGCCACC AGGCCCACCC GCAAACCACG AAGTCAAGGC 
ACAACACCTC ACAGGAGAGA CGCTGGTGCA GACGACCTAC CTCCCCCCCC TGAACCTCCT 
CCCTGTCAGA TCCAGGGTCG CATTCAGGGG ACCCGCAGTG TAAACAGCAT GGCACCACCC 
ACGGACAGGA AGGCTTCGTC TCTGGAGCGC ACGCCCATGT CAGGGCCGCT CTGTGGGCCA 
GACGACACGA AGAGCTCAAT TGACAGACAG ATGCGAACCT CTCTGGAACA TCACCGCCAG 
GCCCAGGACC GACTGGGCTC CATGGACCGG ACTGACGATG CCCGCAGGTG CCACAAATCA 
GAGGATGGGT GTGTCCCGTA TAGCAAGCCA TCCTTCCCCT CCCCCGGGGG CCACAGTTCC 
TCGGGGACGG CGTCCTCCAA GGGGTCAACA GGGCCACGCA AGACCGACGG GCCGCGGCAG 
CCACAACAGT GGACCGCCAC GGAGCATGCC GAATTTCTGG GGGCCAATGG GCATGGAGAG 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_014970471.1 ) cDNA ORF clone, Poecilia mexicana -> XP_014825957.1 Poecilia mexicana roundabout guidance receptor 2 (robo2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo90784
Clone ID Related Accession (Same CDS sequence)	XM_014970471.1
Accession Version	XM_014970471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia mexicana
Product	roundabout homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015096672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia mexicana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGGAG CCTCTCCTGT CTCTTCCTTT GTCATTGAAG CTTTCAGTCA GTCTGTGAGC 
AACAGCTGGC AAACCGTGGC GGACCATGTC AAGACCACCC AGTTCACCGT CAAAGGTCTC 
CGTCCCAACA CCATCTACCT GTTCATGGTC CGAGCCGTCA ACGGCCAAGG CTTAAGTGAT 
CCGAGCCCAA TGTCTGAACC TGTTAGAACT CAAGATATAA GCCCTCCAGC CCAGGGAGTG 
GACCACAGGC ATGTGCAGAA AGAGCTCGGA GAGGTCATAG TTCGCCTGCA TAATCCTGTT 
GTCCTGAGTC CCACCACCAT TCAGGTCACA TGGACAGTGG ACCGCCAGTC CCAGTTCATC 
CAGGGTTACC GGGTTCTATA CAGGCAGACG TCTGGGCTTC CTTCGCCAGG GGCGTGGCAG 
ACCCAGGACG TGAAGGTGCC CTCGGAACGC AGCGTCGTCC TGTCATCGCT GAAGAAGGGC 
ATCGTCTATG AGATCAAGGT CCGGCCGTAC TTCAACGAGT TTCAAGGCAT GGACAGCGAA 
TCGAAGACGG CGCGGACCAC TGAGGAGGCT CCCAGCGCCC CCCCTCAGCA GGTAACCGTC 
CTGACAGTGG GAAACCAGAA CAGCACCTCC ATCAGCATCT CCTGGGACCC CCCTCCGCAC 
GACCACCAGA ACGGCATTAT CCAGGAGTAC AAGATCTGGT GTCTGGGGAA CGAGACGCGT 
TTCCACGTGA ACAAGACGGT GGACGCAGCC ATCCGCTCCG TGGTGGTGGG AGGGCTGCAG 
GTGGGCGTGG TGTATCACGT GGAGGTTGCC GCCAGCACCA GTGCAGGAGT GGGAGTGAAG 
AGTGAACCTC AGTCTATAAT CATCGGGAAA GAGTTCCGGG ACGTGATCAT ACACGGAAAC 
CGGAACAACA GCATCACGGA GCAGATAACG GACGTGGTGA AGCAGCCGGC CTTCATCGCT 
GGCATTGGAG GCGCCTGCTG GGTCATCCTG ATGGGCTTCA GCATCTGGCT CTACTGGAGG 
AGGAAGAAGA GAAAGGGTCT GAGCAACTAT GCAGTTCAGT CCTTCACCTT CACCCCTGCA 
GTGACGTTCC AAAGAGTCGA CGGCGGTCTG ATGAGCAATG GAAGCCGTCC AGGCCTGTTG 
AACGCCAGTG ACCCGGGCTA CCCCTGGTTA GCCGACTCCT GGCCAGCGAC CAGCCTGCCC 
GTCAACAGCA GCTCCGGAGG ACCAAACGAC TTGACTAAAT TTGGTCGAGG AGATCTCCCC 
ACTGGACAGG GAGATAAAAC AGGCACCATG CTGTCTGACG GAGCCATCTA CAGCAGCATA 
GACTTTACCA CCAAGGCTAA CTACAACAGT CCTGGTCAGA CATCCCAAGC CAATCCTTAT 
GCCACCACTC AGATCCTCCA CTCCAGCAGC ATCCATGAGC TGGCTGTGGA CCTGCCTGAC 
GCCCAGTGGA AGGCCTCACT TCAGACTAAG CAAGAAATGG CAAACCTGGG CTACTCCCTG 
CCTGACAAGA ACTCCTGCAA CAACACCCTC CTTTTCATTC CCGACTACCG ATTGGCCGAC 
GGATTGTCTA ATAGAATCCC ACACAACCAA TCACAAGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AGCGAAGCGG CAGTCTCTCA GGTGGAAAAG GAGGGAAGAA GAAGAAAACC 
AAGGCTGGAT CTAAACCTCC CAAAGCTAAT GGCTCCAACT GGGCCAATGT TCCCCTGCCC 
CCTCCCCCGA TTCACCCTCT ACCCGGCACA GAGATGGACC TGTATCCCAA TGAGCACCAT 
GACGGAGGAG GGTATGAGAA TGACACCTGG GGCCCACCCA TGCCTGTGCA GACGTACCTT 
CATCAAGGCA TGGAAGATGA GCTGGAAGAA GAGGAGGAAA GGGTTCCCAC GCCACCCATC 
CGAGGAGTGG CCTCCTCACC GGCTGCCGTC TCTTTTGGAC AGCAGTCCAC AGCCACCCTC 
ACCCCATCTC CCCGGGATGA GATGCAGCCC ATGCTCCAGG CCCATCTGGA TGAGCTGACC 
AGAGCATACC AACTGGACAT GGCTAAACAG ACATGGCACA TGCAGGGAGG CTCTCGTCCT 
CCCCTGGCAC CAGCTCCGCC AGTGGGCTAT GTGTCGAGCA CAATGGTTTC CGACCTGGAG 
ACTGACCTGC AAGACGAGGA GGACGAAGAG GAGGACGAGG ACGAGGATGA GGGCTACGGA 
GCCAGACATC CCCGTGGTAT CGAGCACACA CCAGGGTCTA GTATGGACAA CCTGGACAGC 
TCTATAACAG GGTCCATGGT GAATGGGTGG GGCTCGGCCT CGGAGGATGA GCGTAATTTT 
TCCAGCCATA GATCCAGTCT GGGCAGTTCT TCCGATGGCT CCATCTTTAC CCACTGCAGC 
TTTGCCCAGG CCCTTGTGGC TGCTGCAGAC AAGGCAGGCT ACCGCCTTGA GGGAACCAGC 
CTCAGCAAGA GAGGTAAAGG CTTGTCCCAC AGGCCCCGTC CCAGCAGTCC ATTTTCAACA 
GATGGCAGCA CAGTCGGCAC ACACAGCTTG GGCCACCAGA GGGCCACCAG GCCCACCCGC 
AAACCACGAA GTCAAGGCAC AACACCTCAC AGGAGAGACG CTGGTGCAGA CGACCTACCT 
CCCCCCCCTG AACCTCCTCC CTGTCAGATC CAGGGTCGCA TTCAGGGGAC CCGCAGTGTA 
AACAGCATGG CACCACCCAC GGACAGGAAG GCTTCGTCTC TGGAGCGCAC GCCCATGTCA 
GGGCCGCTCT GTGGGCCAGA CGACACGAAG AGCTCAATTG ACAGACAGAT GCGAACCTCT 
CTGGAACATC ACCGCCAGGC CCAGGACCGA CTGGGCTCCA TGGACCGGAC TGACGATGCC 
CGCAGGTGCC ACAAATCAGA GGATGGGTGT GTCCCGTATA GCAAGCCATC CTTCCCCTCC 
CCCGGGGGCC ACAGTTCCTC GGGGACGGCG TCCTCCAAGG GGTCAACAGG GCCACGCAAG 
ACCGACGGGC CGCGGCAGCC ACAACAGTGG ACCGCCACGG AGCATGCCGA ATTTCTGGGG 
GCCAATGGGC ATGGAGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014825957.1
CDS	144..3227
Translation	MTGASPVSSFVIEAFSQSVSNSWQTVADHVKTTQFTVKGLRPNTIYLFMVRAVNGQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQTSGLPSPGAWQTQDVKVPSERSVVLSSLKKGIVYEIKVRPYFNEFQGMDSESKTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPHDHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQVGVVYHVEVAASTSAGVGVKSEPQSIIIGKEFRDVIIHGNRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDPGYPWLADSWPATSLPVNSSSGGPNDLTKFGRGDLPTGQGDKTGTMLSDGAIYSSIDFTTKANYNSPGQTSQANPYATTQILHSSSIHELAVDLPDAQWKASLQTKQEMANLGYSLPDKNSCNNTLLFIPDYRLADGLSNRIPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKAGSKPPKANGSNWANVPLPPPPIHPLPGTEMDLYPNEHHDGGGYENDTWGPPMPVQTYLHQGMEDELEEEEERVPTPPIRGVASSPAAVSFGQQSTATLTPSPRDEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGSRPPLAPAPPVGYVSSTMVSDLETDLQDEEDEEEDEDEDEGYGARHPRGIEHTPGSSMDNLDSSITGSMVNGWGSASEDERNFSSHRSSLGSSSDGSIFTHCSFAQALVAAADKAGYRLEGTSLSKRGKGLSHRPRPSSPFSTDGSTVGTHSLGHQRATRPTRKPRSQGTTPHRRDAGADDLPPPPEPPPCQIQGRIQGTRSVNSMAPPTDRKASSLERTPMSGPLCGPDDTKSSIDRQMRTSLEHHRQAQDRLGSMDRTDDARRCHKSEDGCVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTDGPRQPQQWTATEHAEFLGANGHGEL

Target ORF information:

RefSeq Version	XM_014970471.1
Organism	Poecilia mexicana
Definition	Poecilia mexicana roundabout guidance receptor 2 (robo2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014970471.1

ORF Insert Sequence:

ATGACCGGAG CCTCTCCTGT CTCTTCCTTT GTCATTGAAG CTTTCAGTCA GTCTGTGAGC 
AACAGCTGGC AAACCGTGGC GGACCATGTC AAGACCACCC AGTTCACCGT CAAAGGTCTC 
CGTCCCAACA CCATCTACCT GTTCATGGTC CGAGCCGTCA ACGGCCAAGG CTTAAGTGAT 
CCGAGCCCAA TGTCTGAACC TGTTAGAACT CAAGATATAA GCCCTCCAGC CCAGGGAGTG 
GACCACAGGC ATGTGCAGAA AGAGCTCGGA GAGGTCATAG TTCGCCTGCA TAATCCTGTT 
GTCCTGAGTC CCACCACCAT TCAGGTCACA TGGACAGTGG ACCGCCAGTC CCAGTTCATC 
CAGGGTTACC GGGTTCTATA CAGGCAGACG TCTGGGCTTC CTTCGCCAGG GGCGTGGCAG 
ACCCAGGACG TGAAGGTGCC CTCGGAACGC AGCGTCGTCC TGTCATCGCT GAAGAAGGGC 
ATCGTCTATG AGATCAAGGT CCGGCCGTAC TTCAACGAGT TTCAAGGCAT GGACAGCGAA 
TCGAAGACGG CGCGGACCAC TGAGGAGGCT CCCAGCGCCC CCCCTCAGCA GGTAACCGTC 
CTGACAGTGG GAAACCAGAA CAGCACCTCC ATCAGCATCT CCTGGGACCC CCCTCCGCAC 
GACCACCAGA ACGGCATTAT CCAGGAGTAC AAGATCTGGT GTCTGGGGAA CGAGACGCGT 
TTCCACGTGA ACAAGACGGT GGACGCAGCC ATCCGCTCCG TGGTGGTGGG AGGGCTGCAG 
GTGGGCGTGG TGTATCACGT GGAGGTTGCC GCCAGCACCA GTGCAGGAGT GGGAGTGAAG 
AGTGAACCTC AGTCTATAAT CATCGGGAAA GAGTTCCGGG ACGTGATCAT ACACGGAAAC 
CGGAACAACA GCATCACGGA GCAGATAACG GACGTGGTGA AGCAGCCGGC CTTCATCGCT 
GGCATTGGAG GCGCCTGCTG GGTCATCCTG ATGGGCTTCA GCATCTGGCT CTACTGGAGG 
AGGAAGAAGA GAAAGGGTCT GAGCAACTAT GCAGTTCAGT CCTTCACCTT CACCCCTGCA 
GTGACGTTCC AAAGAGTCGA CGGCGGTCTG ATGAGCAATG GAAGCCGTCC AGGCCTGTTG 
AACGCCAGTG ACCCGGGCTA CCCCTGGTTA GCCGACTCCT GGCCAGCGAC CAGCCTGCCC 
GTCAACAGCA GCTCCGGAGG ACCAAACGAC TTGACTAAAT TTGGTCGAGG AGATCTCCCC 
ACTGGACAGG GAGATAAAAC AGGCACCATG CTGTCTGACG GAGCCATCTA CAGCAGCATA 
GACTTTACCA CCAAGGCTAA CTACAACAGT CCTGGTCAGA CATCCCAAGC CAATCCTTAT 
GCCACCACTC AGATCCTCCA CTCCAGCAGC ATCCATGAGC TGGCTGTGGA CCTGCCTGAC 
GCCCAGTGGA AGGCCTCACT TCAGACTAAG CAAGAAATGG CAAACCTGGG CTACTCCCTG 
CCTGACAAGA ACTCCTGCAA CAACACCCTC CTTTTCATTC CCGACTACCG ATTGGCCGAC 
GGATTGTCTA ATAGAATCCC ACACAACCAA TCACAAGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AGCGAAGCGG CAGTCTCTCA GGTGGAAAAG GAGGGAAGAA GAAGAAAACC 
AAGGCTGGAT CTAAACCTCC CAAAGCTAAT GGCTCCAACT GGGCCAATGT TCCCCTGCCC 
CCTCCCCCGA TTCACCCTCT ACCCGGCACA GAGATGGACC TGTATCCCAA TGAGCACCAT 
GACGGAGGAG GGTATGAGAA TGACACCTGG GGCCCACCCA TGCCTGTGCA GACGTACCTT 
CATCAAGGCA TGGAAGATGA GCTGGAAGAA GAGGAGGAAA GGGTTCCCAC GCCACCCATC 
CGAGGAGTGG CCTCCTCACC GGCTGCCGTC TCTTTTGGAC AGCAGTCCAC AGCCACCCTC 
ACCCCATCTC CCCGGGATGA GATGCAGCCC ATGCTCCAGG CCCATCTGGA TGAGCTGACC 
AGAGCATACC AACTGGACAT GGCTAAACAG ACATGGCACA TGCAGGGAGG CTCTCGTCCT 
CCCCTGGCAC CAGCTCCGCC AGTGGGCTAT GTGTCGAGCA CAATGGTTTC CGACCTGGAG 
ACTGACCTGC AAGACGAGGA GGACGAAGAG GAGGACGAGG ACGAGGATGA GGGCTACGGA 
GCCAGACATC CCCGTGGTAT CGAGCACACA CCAGGGTCTA GTATGGACAA CCTGGACAGC 
TCTATAACAG GGTCCATGGT GAATGGGTGG GGCTCGGCCT CGGAGGATGA GCGTAATTTT 
TCCAGCCATA GATCCAGTCT GGGCAGTTCT TCCGATGGCT CCATCTTTAC CCACTGCAGC 
TTTGCCCAGG CCCTTGTGGC TGCTGCAGAC AAGGCAGGCT ACCGCCTTGA GGGAACCAGC 
CTCAGCAAGA GAGGTAAAGG CTTGTCCCAC AGGCCCCGTC CCAGCAGTCC ATTTTCAACA 
GATGGCAGCA CAGTCGGCAC ACACAGCTTG GGCCACCAGA GGGCCACCAG GCCCACCCGC 
AAACCACGAA GTCAAGGCAC AACACCTCAC AGGAGAGACG CTGGTGCAGA CGACCTACCT 
CCCCCCCCTG AACCTCCTCC CTGTCAGATC CAGGGTCGCA TTCAGGGGAC CCGCAGTGTA 
AACAGCATGG CACCACCCAC GGACAGGAAG GCTTCGTCTC TGGAGCGCAC GCCCATGTCA 
GGGCCGCTCT GTGGGCCAGA CGACACGAAG AGCTCAATTG ACAGACAGAT GCGAACCTCT 
CTGGAACATC ACCGCCAGGC CCAGGACCGA CTGGGCTCCA TGGACCGGAC TGACGATGCC 
CGCAGGTGCC ACAAATCAGA GGATGGGTGT GTCCCGTATA GCAAGCCATC CTTCCCCTCC 
CCCGGGGGCC ACAGTTCCTC GGGGACGGCG TCCTCCAAGG GGTCAACAGG GCCACGCAAG 
ACCGACGGGC CGCGGCAGCC ACAACAGTGG ACCGCCACGG AGCATGCCGA ATTTCTGGGG 
GCCAATGGGC ATGGAGAGTT ATAG