LOC109726401 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109726401
Entrez Gene ID	109726401
Full Name	DNA topoisomerase 1 beta-like
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020255969.1	XP_020111558.1	DNA topoisomerase 1 beta-like isoform X1
XM_020255970.1	XP_020111559.1	DNA topoisomerase 1 alpha-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109726401 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109726401 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn82213	XM_020255969.1 Latest version!	Ananas comosus DNA topoisomerase 1 beta-like (LOC109726401), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OAn82214	XM_020255970.1 Latest version!	Ananas comosus DNA topoisomerase 1 beta-like (LOC109726401), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109726401 ( XM_020255969.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020111558.1 Ananas comosus DNA topoisomerase 1 beta-like (LOC109726401), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn82213
Clone ID Related Accession (Same CDS sequence)	XM_020255969.1
Accession Version	XM_020255969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	DNA topoisomerase 1 beta-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033641.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTG ATGGGTGTGT CAATCCTGTT ATTTATGATA ATGGTGATGA CGATGATGCC 
CCTCTTACTT TTAAAAGATC AAGCTCTTCG AGACATAGTT CATTAAATTC TACTTCAAAC 
AAATCGACTT CCCAAAAGCG TGATATCTCT CTTCAGCGGG CTAGATCTGA TCATAGTTCT 
CCTAATGGTG AGAATTCAAA CTTGCAAAAG AGTAAGGGGA CTTCTACTTC AAAGATGCCT 
ACTGAGAGGC CGAAAATACC AAACCCAGTT TCTCGGTCCT CAGTACCTGG TCCGCCCAAC 
CATTCATCAG AAAATAAACA TACTACTAGT TCTATGGATA GGACTAATTT GAAAAGGCCC 
CATGAGAACT CAGATGATTC AGATGATAAT ATACCATTAA GTCAGAGAGC CAATCCAGCT 
TCAAAGATGG TTAAACATTT TTCAGAACAA AGGGACACAT CACATGGTAA AGTTGAGCCT 
TCCAGTGATT CTGATGATGA TAATAAGCCA TTGATCTCCA AGTATTCCTC AGTTGGAGCT 
GGGAAAGTTC CTGCATCTTC ATCAAAGAAC AGAAGCGGTA AGGAAAGTGC TGATGATTCA 
GAAGATAAGA AACCTTTAGC TTCTAAGGTT AAGGCAAATG CATCTACCGC TGGAGCTGAG 
CATGTTGCTT CATCTTCAGC CAGAAAGATA AACAGCAGCA GCAAGGAAAA CGCTGATGAT 
TCAGAAGATG AGAAACCTTT GGCTTTTAAG TTTAAGGCGA ATGCGGCTAC CACTGGAGCT 
GGGCAAGTTG CTTCATCTTC ATCCAAGAAG ATAAACAGCA GCAGCAAGGC TAATGATTCA 
GAAGATAGGA AACCTTTGGC TTCAAAGATT AAGGCAAATA TGTCTATTAA GAAGGAAACA 
GGTAGCGAAT CAAGGTCCCC AAAAGATATA AACAAGCGGA CCCTTGGTGA TGTTAATAAT 
AGAAATGATT CTTTAGTTAA GAAGGCAAAA GTTTCAGATA ACTCTGCTTT TGTAAAGGTA 
AAGCATGAAT CTGTTGTGAA AAAAGAATTA AAAACAGATG ATGGGGATCG CAAACCTCTT 
GCAGAAAAGA TGAGGAAGTT AGGGTCTTCA GACAGCAAAT CATCTACAAC GAAAGTGATA 
AAAAAACCCA CTCCATCATC CAAGAAAGAT GGCAAGAAAT TGAAGATGAA GAAAGCAATG 
AAAAAATCTA CATTTTCAAA GTCATTGAAG GTTCCTCCTG GGTCAGGCGG AGGACAAAAA 
TGGACTACCT TGGAGCACAA CGGTGTTATT TTTCCTCCTC CGTACCAACC TCATGGGGTC 
AAAATGCTCT ACAATGGACA ACCGGTTGAT TTAACTCCAG AGCAAGAGGA GGTTGCAACA 
ATGTTTGCAG TGATGAAAGA TACGGATTAC GCCACGAAGC CGAAATTTAT TCATAACTTT 
TTTAATGACT GGCGGCAAGT TCTTGGTAAA AACCATGTTA TTAAGAAATT TGAGCTTTGT 
GATTTTACTC CAATCTATGA ATGGCATCTA AAGGAGAAGG AGAAAAAGAA GCAAATGAGT 
GCTGCGGAGA AGAAAGCTTT AAAAGAAGAG AAGTTAAAGA AAGAGGACAA GTACATGTGG 
GCTATTGTTG ATGGTGTCAA AGAGAAGGTC GGGAATTTTA GAGTGGAACC ACCTGCGTTA 
TTCCGAGGCC GTGGAGAGCA TCCAAAGATG GGCAAATTAA AACGACGCAT TCGACCAAGT 
GACATCACGA TCAACATTGG AAAAAATGCT CCAATTCCTG AGTGTCCCAT TCCTGGTGAA 
AGATGGAGAG AAGTGAAGCA TGATAACACG GTTACGTGGT TAGCATACTG GAGTGATCCC 
ATAAATCCAA AAGACTCCAA GTATGTCTTT TTGGCAGCAA GCAGCTCTTT AAAAGGGCAA 
AGTGACAAAG AGAAGTATGA GAAGGCTAGG CAATTGAAGG ATTATATACA TAATATTCGA 
TCCACTTACA CGAAGGATTT CAATAGTAAA GATCCTACAA AAAGGCAAAT AGCAGTGGCG 
ACATACCTCA TCGATAAGCT AGCGCTTAGG GCAGGCAATG AGAAGGATGA TGATGAGGCT 
GATACTGTTG GTTGCTGTAC TCTGAAGGTG GAGAATGTCA TCTGTGTGCC CCCAAATCAG 
TTACAGTTTG ACTTCCTTGG TAAAGATTCT ATAAGATATT TTAACACTGT GGAGGTCGAA 
GCACCTGTAT ACAAAGCGAT TGGGGAGTTC CGTACTGCTA AAAAAGGTGA CGGAAAAAGA 
AAAGGCGATG GAGATGATCT CTTTGACTTG CTTGATACAA GTAAACTTAA TGCTCACCTT 
AAGGAACTCA TGCCTGGTCT TACTGCAAAA GTTTTCCGTA CATACAATGC TTCTATTACC 
TTGGATGACA TATTGAATAA GGAAACCAAG GAAGGCACTC TCCCTGAAAA GATCGCTGTT 
TATCAGCGTG CAAACAAAGA GGTCGCTATC ATATGCAATC ATCAGCGCAG TGTTTCGAAG 
TCCCACGAAT CCCAGATGTC TAGATTGAAT GAAAAGATTG ACGAACTAAA GGCTCTACGG 
GAGGAATTGA AGGTGGATTT GAGTAGGGCA AGAAAAGGGA AGCCTCCACT TAAAGACGCA 
GATGGGAAGA CGAAGAGGAA CTTGTCCCCT GAAGCGATAC AGAAGAAGCT TGCACAGACG 
AATGCGAAGA TAGAGAAGAT GGAGCTGGAT AAAAAGATAA AGGAGGATTT GAAGACGGTG 
GCATTGGGGA CGTCTAAGAT CAATTATCTC GACCCTAGAA TATCCGTTGC GTGGTGCAAG 
CGCCATGAAG TTCCCATTGA GAAGATATTC AACAAATCAC TTCTCGCGAA ATTCGCGTGG 
GCAATGGACG TCGACCCCAG CTACAGATTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020111558.1
CDS	205..3117
Translation	MAVDGCVNPVIYDNGDDDDAPLTFKRSSSSRHSSLNSTSNKSTSQKRDISLQRARSDHSSPNGENSNLQKSKGTSTSKMPTERPKIPNPVSRSSVPGPPNHSSENKHTTSSMDRTNLKRPHENSDDSDDNIPLSQRANPASKMVKHFSEQRDTSHGKVEPSSDSDDDNKPLISKYSSVGAGKVPASSSKNRSGKESADDSEDKKPLASKVKANASTAGAEHVASSSARKINSSSKENADDSEDEKPLAFKFKANAATTGAGQVASSSSKKINSSSKANDSEDRKPLASKIKANMSIKKETGSESRSPKDINKRTLGDVNNRNDSLVKKAKVSDNSAFVKVKHESVVKKELKTDDGDRKPLAEKMRKLGSSDSKSSTTKVIKKPTPSSKKDGKKLKMKKAMKKSTFSKSLKVPPGSGGGQKWTTLEHNGVIFPPPYQPHGVKMLYNGQPVDLTPEQEEVATMFAVMKDTDYATKPKFIHNFFNDWRQVLGKNHVIKKFELCDFTPIYEWHLKEKEKKKQMSAAEKKALKEEKLKKEDKYMWAIVDGVKEKVGNFRVEPPALFRGRGEHPKMGKLKRRIRPSDITINIGKNAPIPECPIPGERWREVKHDNTVTWLAYWSDPINPKDSKYVFLAASSSLKGQSDKEKYEKARQLKDYIHNIRSTYTKDFNSKDPTKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVICVPPNQLQFDFLGKDSIRYFNTVEVEAPVYKAIGEFRTAKKGDGKRKGDGDDLFDLLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDILNKETKEGTLPEKIAVYQRANKEVAIICNHQRSVSKSHESQMSRLNEKIDELKALREELKVDLSRARKGKPPLKDADGKTKRNLSPEAIQKKLAQTNAKIEKMELDKKIKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPSYRF

Target ORF information:

RefSeq Version	XM_020255969.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus DNA topoisomerase 1 beta-like (LOC109726401), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020255969.1

ORF Insert Sequence:

ATGGCTGTTG ATGGGTGTGT CAATCCTGTT ATTTATGATA ATGGTGATGA CGATGATGCC 
CCTCTTACTT TTAAAAGATC AAGCTCTTCG AGACATAGTT CATTAAATTC TACTTCAAAC 
AAATCGACTT CCCAAAAGCG TGATATCTCT CTTCAGCGGG CTAGATCTGA TCATAGTTCT 
CCTAATGGTG AGAATTCAAA CTTGCAAAAG AGTAAGGGGA CTTCTACTTC AAAGATGCCT 
ACTGAGAGGC CGAAAATACC AAACCCAGTT TCTCGGTCCT CAGTACCTGG TCCGCCCAAC 
CATTCATCAG AAAATAAACA TACTACTAGT TCTATGGATA GGACTAATTT GAAAAGGCCC 
CATGAGAACT CAGATGATTC AGATGATAAT ATACCATTAA GTCAGAGAGC CAATCCAGCT 
TCAAAGATGG TTAAACATTT TTCAGAACAA AGGGACACAT CACATGGTAA AGTTGAGCCT 
TCCAGTGATT CTGATGATGA TAATAAGCCA TTGATCTCCA AGTATTCCTC AGTTGGAGCT 
GGGAAAGTTC CTGCATCTTC ATCAAAGAAC AGAAGCGGTA AGGAAAGTGC TGATGATTCA 
GAAGATAAGA AACCTTTAGC TTCTAAGGTT AAGGCAAATG CATCTACCGC TGGAGCTGAG 
CATGTTGCTT CATCTTCAGC CAGAAAGATA AACAGCAGCA GCAAGGAAAA CGCTGATGAT 
TCAGAAGATG AGAAACCTTT GGCTTTTAAG TTTAAGGCGA ATGCGGCTAC CACTGGAGCT 
GGGCAAGTTG CTTCATCTTC ATCCAAGAAG ATAAACAGCA GCAGCAAGGC TAATGATTCA 
GAAGATAGGA AACCTTTGGC TTCAAAGATT AAGGCAAATA TGTCTATTAA GAAGGAAACA 
GGTAGCGAAT CAAGGTCCCC AAAAGATATA AACAAGCGGA CCCTTGGTGA TGTTAATAAT 
AGAAATGATT CTTTAGTTAA GAAGGCAAAA GTTTCAGATA ACTCTGCTTT TGTAAAGGTA 
AAGCATGAAT CTGTTGTGAA AAAAGAATTA AAAACAGATG ATGGGGATCG CAAACCTCTT 
GCAGAAAAGA TGAGGAAGTT AGGGTCTTCA GACAGCAAAT CATCTACAAC GAAAGTGATA 
AAAAAACCCA CTCCATCATC CAAGAAAGAT GGCAAGAAAT TGAAGATGAA GAAAGCAATG 
AAAAAATCTA CATTTTCAAA GTCATTGAAG GTTCCTCCTG GGTCAGGCGG AGGACAAAAA 
TGGACTACCT TGGAGCACAA CGGTGTTATT TTTCCTCCTC CGTACCAACC TCATGGGGTC 
AAAATGCTCT ACAATGGACA ACCGGTTGAT TTAACTCCAG AGCAAGAGGA GGTTGCAACA 
ATGTTTGCAG TGATGAAAGA TACGGATTAC GCCACGAAGC CGAAATTTAT TCATAACTTT 
TTTAATGACT GGCGGCAAGT TCTTGGTAAA AACCATGTTA TTAAGAAATT TGAGCTTTGT 
GATTTTACTC CAATCTATGA ATGGCATCTA AAGGAGAAGG AGAAAAAGAA GCAAATGAGT 
GCTGCGGAGA AGAAAGCTTT AAAAGAAGAG AAGTTAAAGA AAGAGGACAA GTACATGTGG 
GCTATTGTTG ATGGTGTCAA AGAGAAGGTC GGGAATTTTA GAGTGGAACC ACCTGCGTTA 
TTCCGAGGCC GTGGAGAGCA TCCAAAGATG GGCAAATTAA AACGACGCAT TCGACCAAGT 
GACATCACGA TCAACATTGG AAAAAATGCT CCAATTCCTG AGTGTCCCAT TCCTGGTGAA 
AGATGGAGAG AAGTGAAGCA TGATAACACG GTTACGTGGT TAGCATACTG GAGTGATCCC 
ATAAATCCAA AAGACTCCAA GTATGTCTTT TTGGCAGCAA GCAGCTCTTT AAAAGGGCAA 
AGTGACAAAG AGAAGTATGA GAAGGCTAGG CAATTGAAGG ATTATATACA TAATATTCGA 
TCCACTTACA CGAAGGATTT CAATAGTAAA GATCCTACAA AAAGGCAAAT AGCAGTGGCG 
ACATACCTCA TCGATAAGCT AGCGCTTAGG GCAGGCAATG AGAAGGATGA TGATGAGGCT 
GATACTGTTG GTTGCTGTAC TCTGAAGGTG GAGAATGTCA TCTGTGTGCC CCCAAATCAG 
TTACAGTTTG ACTTCCTTGG TAAAGATTCT ATAAGATATT TTAACACTGT GGAGGTCGAA 
GCACCTGTAT ACAAAGCGAT TGGGGAGTTC CGTACTGCTA AAAAAGGTGA CGGAAAAAGA 
AAAGGCGATG GAGATGATCT CTTTGACTTG CTTGATACAA GTAAACTTAA TGCTCACCTT 
AAGGAACTCA TGCCTGGTCT TACTGCAAAA GTTTTCCGTA CATACAATGC TTCTATTACC 
TTGGATGACA TATTGAATAA GGAAACCAAG GAAGGCACTC TCCCTGAAAA GATCGCTGTT 
TATCAGCGTG CAAACAAAGA GGTCGCTATC ATATGCAATC ATCAGCGCAG TGTTTCGAAG 
TCCCACGAAT CCCAGATGTC TAGATTGAAT GAAAAGATTG ACGAACTAAA GGCTCTACGG 
GAGGAATTGA AGGTGGATTT GAGTAGGGCA AGAAAAGGGA AGCCTCCACT TAAAGACGCA 
GATGGGAAGA CGAAGAGGAA CTTGTCCCCT GAAGCGATAC AGAAGAAGCT TGCACAGACG 
AATGCGAAGA TAGAGAAGAT GGAGCTGGAT AAAAAGATAA AGGAGGATTT GAAGACGGTG 
GCATTGGGGA CGTCTAAGAT CAATTATCTC GACCCTAGAA TATCCGTTGC GTGGTGCAAG 
CGCCATGAAG TTCCCATTGA GAAGATATTC AACAAATCAC TTCTCGCGAA ATTCGCGTGG 
GCAATGGACG TCGACCCCAG CTACAGATTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109726401 ( XM_020255970.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020111559.1 Ananas comosus DNA topoisomerase 1 beta-like (LOC109726401), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn82214
Clone ID Related Accession (Same CDS sequence)	XM_020255970.1
Accession Version	XM_020255970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	DNA topoisomerase 1 alpha-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033641.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTG ATGGGTGTGT CAATCCTGTT ATTTATGATA ATGGTGATGA CGATGATGCC 
CCTCTTACTT TTAAAAGATC AAGCTCTTCG AGACATAGTT CATTAAATTC TACTTCAAAC 
AAATCGACTT CCCAAAAGCG TGATATCTCT CTTCAGCGGG CTAGATCTGA TCATAGTTCT 
CCTAATGGTG AGAATTCAAA CTTGCAAAAG AGTAAGGGGA CTTCTACTTC AAAGATGCCT 
ACTGAGAGGC CGAAAATACC AAACCCAGTT TCTCGGTCCT CAGTACCTGG TCCGCCCAAC 
CATTCATCAG AAAATAAACA TACTACTAGT TCTATGGATA GGACTAATTT GAAAAGGCCC 
CATGAGAACT CAGATGATTC AGATGATAAT ATACCATTAA GTCAGAGAGC CAATCCAGCT 
TCAAAGATGG TTAAACATTT TTCAGAACAA AGGGACACAT CACATGGTAA AGTTGAGCCT 
TCCAGTGATT CTGATGATGA TAATAAGCCA TTGATCTCCA AGTATTCCTC AGTTGGAGCT 
GGGAAAGTTC CTGCATCTTC ATCAAAGAAC AGAAGCGGTA AGGAAAGTGC TGATGATTCA 
GAAGATAAGA AACCTTTAGC TTCTAAGGTT AAGGCAAATG CATCTACCGC TGGAGCTGAG 
CATGTTGCTT CATCTTCAGC CAGAAAGATA AACAGCAGCA GCAAGGAAAA CGCTGATGAT 
TCAGAAGATG AGAAACCTTT GGCTTTTAAG TTTAAGGCGA ATGCGGCTAC CACTGGAGCT 
GGGCAAGTTG CTTCATCTTC ATCCAAGAAG ATAAACAGCA GCAGCAAGGC TAATGATTCA 
GAAGATAGGA AACCTTTGGC TTCAAAGATT AAGGCAAATA TGTCTATTAA GAAGGAAACA 
GGTAGCGAAT CAAGGTCCCC AAAAGATATA AACAAGCGGA CCCTTGGTGA TGTTAATAAT 
AGAAATGATT CTTTAGTTAA GAAGGCAAAA GTTTCAGATA ACTCTGCTTT TGTAAAGGTA 
AAGCATGAAT CTGTTGTGAA AAAAGAATTA AAAACAGATG ATGGGGATCG CAAACCTCTT 
GCAGAAAAGA TGAGGAAGTT AGGGTCTTCA GACAGCAAAT CATCTACAAC GAAAGTGATA 
AAAAAACCCA CTCCATCATC CAAGAAAGAT GGCAAGAAAT TGAAGATGAA GAAAGCAATG 
AAAAAATCTA CATTTTCAAA GTCATTGAAG GTTCCTCCTG GGTCAGGCGG AGGACAAAAA 
TGGACTACCT TGGAGCACAA CGGTGTTATT TTTCCTCCTC CGTACCAACC TCATGGGGTC 
AAAATGCTCT ACAATGGACA ACCGGTTGAT TTAACTCCAG AGCAAGAGGA GGTTGCAACA 
ATGTTTGCAG TGATGAAAGA TACGGATTAC GCCACGAAGC CGAAATTTAT TCATAACTTT 
TTTAATGACT GGCGGCAAGT TCTTGGTAAA AACCATGTTA TTAAGAAATT TGAGCTTTGT 
GATTTTACTC CAATCTATGA ATGGCATCTA AAGGAGAAGG AGAAAAAGAA GCAAATGAGT 
GCTGCGGAGA AGAAAGCTTT AAAAGAAGAG AAGTTAAAGA AAGAGGACAA GTACATGTGG 
GCTATTGTTG ATGGTGTCAA AGAGAAGGTC GGGAATTTTA GAGTGGAACC ACCTGCGTTA 
TTCCGAGGCC GTGGAGAGCA TCCAAAGATG GGCAAATTAA AACGACGCAT TCGACCAAGT 
GACATCACGA TCAACATTGG AAAAAATGCT CCAATTCCTG AGTGTCCCAT TCCTGGTGAA 
AGATGGAGAG AAGTGAAGCA TGATAACACG GTTACGTGGT TAGCATACTG GAGTGATCCC 
ATAAATCCAA AAGACTCCAA GTATGTCTTT TTGGCAGCAA GCAGCTCTTT AAAAGGGCAA 
AGTGACAAAG AGAAGTATGA GAAGGCTAGG CAATTGAAGG ATTATATACA TAATATTCGA 
TCCACTTACA CGAAGGATTT CAATAGTAAA GATCCTACAA AAAGGCAAAT AGCAGTGGCG 
ACATACCTCA TCGATAAGCT AGCGCTTAGG GCAGGCAATG AGAAGGATGA TGATGAGGCT 
GATACTGTTG GTTGCTGTAC TCTGAAGGTG GAGAATGTCA TCTGTGTGCC CCCAAATCAG 
TTACAGTTTG ACTTCCTTGG TAAAGATTCT ATAAGATATT TTAACACTGT GGAGGTCGAA 
GCACCTGTAT ACAAAGCGAT TGGGGAGTTC CGTACTGCTA AAAAAGGTGA CGGAAAAAGA 
AAAGGCGATG GAGATGATCT CTTTGACTTG CTTGATACAA GTAAACTTAA TGCTCACCTT 
AAGGAACTCA TGCCTGGTCT TACTGCAAAA GTTTTCCGTA CATACAATGC TTCTATTACC 
TTGGATGACA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020111559.1
CDS	209..2623
Translation	MAVDGCVNPVIYDNGDDDDAPLTFKRSSSSRHSSLNSTSNKSTSQKRDISLQRARSDHSSPNGENSNLQKSKGTSTSKMPTERPKIPNPVSRSSVPGPPNHSSENKHTTSSMDRTNLKRPHENSDDSDDNIPLSQRANPASKMVKHFSEQRDTSHGKVEPSSDSDDDNKPLISKYSSVGAGKVPASSSKNRSGKESADDSEDKKPLASKVKANASTAGAEHVASSSARKINSSSKENADDSEDEKPLAFKFKANAATTGAGQVASSSSKKINSSSKANDSEDRKPLASKIKANMSIKKETGSESRSPKDINKRTLGDVNNRNDSLVKKAKVSDNSAFVKVKHESVVKKELKTDDGDRKPLAEKMRKLGSSDSKSSTTKVIKKPTPSSKKDGKKLKMKKAMKKSTFSKSLKVPPGSGGGQKWTTLEHNGVIFPPPYQPHGVKMLYNGQPVDLTPEQEEVATMFAVMKDTDYATKPKFIHNFFNDWRQVLGKNHVIKKFELCDFTPIYEWHLKEKEKKKQMSAAEKKALKEEKLKKEDKYMWAIVDGVKEKVGNFRVEPPALFRGRGEHPKMGKLKRRIRPSDITINIGKNAPIPECPIPGERWREVKHDNTVTWLAYWSDPINPKDSKYVFLAASSSLKGQSDKEKYEKARQLKDYIHNIRSTYTKDFNSKDPTKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVICVPPNQLQFDFLGKDSIRYFNTVEVEAPVYKAIGEFRTAKKGDGKRKGDGDDLFDLLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDI

Target ORF information:

RefSeq Version	XM_020255970.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus DNA topoisomerase 1 beta-like (LOC109726401), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020255970.1

ORF Insert Sequence:

ATGGCTGTTG ATGGGTGTGT CAATCCTGTT ATTTATGATA ATGGTGATGA CGATGATGCC 
CCTCTTACTT TTAAAAGATC AAGCTCTTCG AGACATAGTT CATTAAATTC TACTTCAAAC 
AAATCGACTT CCCAAAAGCG TGATATCTCT CTTCAGCGGG CTAGATCTGA TCATAGTTCT 
CCTAATGGTG AGAATTCAAA CTTGCAAAAG AGTAAGGGGA CTTCTACTTC AAAGATGCCT 
ACTGAGAGGC CGAAAATACC AAACCCAGTT TCTCGGTCCT CAGTACCTGG TCCGCCCAAC 
CATTCATCAG AAAATAAACA TACTACTAGT TCTATGGATA GGACTAATTT GAAAAGGCCC 
CATGAGAACT CAGATGATTC AGATGATAAT ATACCATTAA GTCAGAGAGC CAATCCAGCT 
TCAAAGATGG TTAAACATTT TTCAGAACAA AGGGACACAT CACATGGTAA AGTTGAGCCT 
TCCAGTGATT CTGATGATGA TAATAAGCCA TTGATCTCCA AGTATTCCTC AGTTGGAGCT 
GGGAAAGTTC CTGCATCTTC ATCAAAGAAC AGAAGCGGTA AGGAAAGTGC TGATGATTCA 
GAAGATAAGA AACCTTTAGC TTCTAAGGTT AAGGCAAATG CATCTACCGC TGGAGCTGAG 
CATGTTGCTT CATCTTCAGC CAGAAAGATA AACAGCAGCA GCAAGGAAAA CGCTGATGAT 
TCAGAAGATG AGAAACCTTT GGCTTTTAAG TTTAAGGCGA ATGCGGCTAC CACTGGAGCT 
GGGCAAGTTG CTTCATCTTC ATCCAAGAAG ATAAACAGCA GCAGCAAGGC TAATGATTCA 
GAAGATAGGA AACCTTTGGC TTCAAAGATT AAGGCAAATA TGTCTATTAA GAAGGAAACA 
GGTAGCGAAT CAAGGTCCCC AAAAGATATA AACAAGCGGA CCCTTGGTGA TGTTAATAAT 
AGAAATGATT CTTTAGTTAA GAAGGCAAAA GTTTCAGATA ACTCTGCTTT TGTAAAGGTA 
AAGCATGAAT CTGTTGTGAA AAAAGAATTA AAAACAGATG ATGGGGATCG CAAACCTCTT 
GCAGAAAAGA TGAGGAAGTT AGGGTCTTCA GACAGCAAAT CATCTACAAC GAAAGTGATA 
AAAAAACCCA CTCCATCATC CAAGAAAGAT GGCAAGAAAT TGAAGATGAA GAAAGCAATG 
AAAAAATCTA CATTTTCAAA GTCATTGAAG GTTCCTCCTG GGTCAGGCGG AGGACAAAAA 
TGGACTACCT TGGAGCACAA CGGTGTTATT TTTCCTCCTC CGTACCAACC TCATGGGGTC 
AAAATGCTCT ACAATGGACA ACCGGTTGAT TTAACTCCAG AGCAAGAGGA GGTTGCAACA 
ATGTTTGCAG TGATGAAAGA TACGGATTAC GCCACGAAGC CGAAATTTAT TCATAACTTT 
TTTAATGACT GGCGGCAAGT TCTTGGTAAA AACCATGTTA TTAAGAAATT TGAGCTTTGT 
GATTTTACTC CAATCTATGA ATGGCATCTA AAGGAGAAGG AGAAAAAGAA GCAAATGAGT 
GCTGCGGAGA AGAAAGCTTT AAAAGAAGAG AAGTTAAAGA AAGAGGACAA GTACATGTGG 
GCTATTGTTG ATGGTGTCAA AGAGAAGGTC GGGAATTTTA GAGTGGAACC ACCTGCGTTA 
TTCCGAGGCC GTGGAGAGCA TCCAAAGATG GGCAAATTAA AACGACGCAT TCGACCAAGT 
GACATCACGA TCAACATTGG AAAAAATGCT CCAATTCCTG AGTGTCCCAT TCCTGGTGAA 
AGATGGAGAG AAGTGAAGCA TGATAACACG GTTACGTGGT TAGCATACTG GAGTGATCCC 
ATAAATCCAA AAGACTCCAA GTATGTCTTT TTGGCAGCAA GCAGCTCTTT AAAAGGGCAA 
AGTGACAAAG AGAAGTATGA GAAGGCTAGG CAATTGAAGG ATTATATACA TAATATTCGA 
TCCACTTACA CGAAGGATTT CAATAGTAAA GATCCTACAA AAAGGCAAAT AGCAGTGGCG 
ACATACCTCA TCGATAAGCT AGCGCTTAGG GCAGGCAATG AGAAGGATGA TGATGAGGCT 
GATACTGTTG GTTGCTGTAC TCTGAAGGTG GAGAATGTCA TCTGTGTGCC CCCAAATCAG 
TTACAGTTTG ACTTCCTTGG TAAAGATTCT ATAAGATATT TTAACACTGT GGAGGTCGAA 
GCACCTGTAT ACAAAGCGAT TGGGGAGTTC CGTACTGCTA AAAAAGGTGA CGGAAAAAGA 
AAAGGCGATG GAGATGATCT CTTTGACTTG CTTGATACAA GTAAACTTAA TGCTCACCTT 
AAGGAACTCA TGCCTGGTCT TACTGCAAAA GTTTTCCGTA CATACAATGC TTCTATTACC 
TTGGATGACA TATAA