AT1G75890 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following AT1G75890 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT1G75890 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb28075 NM_001084360.1
Latest version!
Arabidopsis thaliana GDSL-like Lipase/Acylhydrolase superfamily protein (AT1G75890), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OAb28076 NM_106239.2
Latest version!
Arabidopsis thaliana GDSL-like Lipase/Acylhydrolase superfamily protein (AT1G75890), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OAb34984 NM_001334701.1
Latest version!
Arabidopsis thaliana GDSL-like Lipase/Acylhydrolase superfamily protein (AT1G75890), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb28075
    Clone ID Related Accession (Same CDS sequence) NM_001084360.1
    Accession Version NM_001084360.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product GDSL-like Lipase/Acylhydrolase superfamily protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). COMPLETENESS: incomplete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAACGAA ACAGTATAAA TATTCATCAC GTTACTTCTT TTTCTTCGAG TCCGTTTTGG 
    TGTGTTTTCT TTCTCGTGTT GTTATGTAAG ACGTCCACCA ACGCTTTGGT GAAGCAGCCG
    CCAAACGAAA CGACTCCGGC GATAATTGTG TTCGGAGATT CAATCGTTGA TGCCGGAAAT
    AATGACGACA TTATGACAAC GTTGGCTAGA TGCAATTATC CTCCTTACGG CATCGATTTT
    GACGGTGGAA TTCCTACCGG AAGATTTTGC AACGGCAAAG TTGCGACTGA TTTTATAGCG
    GGAAAATTCG GAATTAAACC AAGTATACCG GCATATCGAA ATCCAAATTT GAAACCAGAA
    GATCTCTTAA CCGGTGTAAC ATTTGCGTCT GGTGGTGCTG GTTACGTTCC CTTCACAACC
    CAATTATCGG GAGGAATAGC TTTATCGCAG CAACTGAAGT TATTCGAAGA GTATGTAGAG
    AAGATGAAGA AAATGGTTGG GGAAGAAAGG ACAAAGCTCA TAATTAAAAA CAGTTTGTTC
    ATGGTTATAT GTGGCAGTAA TGATATAACC AACACCTACT TTGGTCTTCC TTCTGTTCAA
    CAACAATACG ACGTCGCTTC CTTTACCACT CTTATGGCCG ACAATGCTCG ATCTTTTGCT
    CAAAAATTAC ATGAATATGG AGCGAGGAGA ATACAAGTAT TCGGTGCACC ACCAGTAGGA
    TGTGTCCCAT CACAGAGAAC CTTAGCAGGA GGGCCAACAA GAAATTGTGT TGTTAGGTTC
    AATGATGCAA CAAAGCTCTA CAATGTTAAG CTCGCTGCAA ATTTGGGTTC TTTGTCAAGA
    ACTTTAGGAG ACAAGACAAT AATCTACGTT GACATATATG ATTCTCTCCT TGATATCATT
    CTTGACCCTC GACAATATGG ATTTAAGGTG GTTGATAAAG GATGTTGTGG AACTGGACTA
    ATTGAAGTTG CTCTTTTATG CAACAACTTT GCAGCTGATG TATGTCCAAA TAGAGATGAA
    TATGTGTTTT GGGATAGTTT CCATCCGACT GAGAAGACTT ACAGAATTAT GGCCACAAAA
    TATTTTGAGA GATATGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077829.1
    CDS21..1121
    Translation

    Target ORF information:

    RefSeq Version NM_001084360.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana GDSL-like Lipase/Acylhydrolase superfamily protein (AT1G75890), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001084360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAACGAA ACAGTATAAA TATTCATCAC GTTACTTCTT TTTCTTCGAG TCCGTTTTGG 
    TGTGTTTTCT TTCTCGTGTT GTTATGTAAG ACGTCCACCA ACGCTTTGGT GAAGCAGCCG
    CCAAACGAAA CGACTCCGGC GATAATTGTG TTCGGAGATT CAATCGTTGA TGCCGGAAAT
    AATGACGACA TTATGACAAC GTTGGCTAGA TGCAATTATC CTCCTTACGG CATCGATTTT
    GACGGTGGAA TTCCTACCGG AAGATTTTGC AACGGCAAAG TTGCGACTGA TTTTATAGCG
    GGAAAATTCG GAATTAAACC AAGTATACCG GCATATCGAA ATCCAAATTT GAAACCAGAA
    GATCTCTTAA CCGGTGTAAC ATTTGCGTCT GGTGGTGCTG GTTACGTTCC CTTCACAACC
    CAATTATCGG GAGGAATAGC TTTATCGCAG CAACTGAAGT TATTCGAAGA GTATGTAGAG
    AAGATGAAGA AAATGGTTGG GGAAGAAAGG ACAAAGCTCA TAATTAAAAA CAGTTTGTTC
    ATGGTTATAT GTGGCAGTAA TGATATAACC AACACCTACT TTGGTCTTCC TTCTGTTCAA
    CAACAATACG ACGTCGCTTC CTTTACCACT CTTATGGCCG ACAATGCTCG ATCTTTTGCT
    CAAAAATTAC ATGAATATGG AGCGAGGAGA ATACAAGTAT TCGGTGCACC ACCAGTAGGA
    TGTGTCCCAT CACAGAGAAC CTTAGCAGGA GGGCCAACAA GAAATTGTGT TGTTAGGTTC
    AATGATGCAA CAAAGCTCTA CAATGTTAAG CTCGCTGCAA ATTTGGGTTC TTTGTCAAGA
    ACTTTAGGAG ACAAGACAAT AATCTACGTT GACATATATG ATTCTCTCCT TGATATCATT
    CTTGACCCTC GACAATATGG ATTTAAGGTG GTTGATAAAG GATGTTGTGG AACTGGACTA
    ATTGAAGTTG CTCTTTTATG CAACAACTTT GCAGCTGATG TATGTCCAAA TAGAGATGAA
    TATGTGTTTT GGGATAGTTT CCATCCGACT GAGAAGACTT ACAGAATTAT GGCCACAAAA
    TATTTTGAGA GATATGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb28076
    Clone ID Related Accession (Same CDS sequence) NM_106239.2
    Accession Version NM_106239.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product GDSL-like Lipase/Acylhydrolase superfamily protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_106239.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAACGAA ACAGTATAAA TATTCATCAC GTTACTTCTT TTTCTTCGAG TCCGTTTTGG 
    TGTGTTTTCT TTCTCGTGTT GTTATGTAAG ACGTCCACCA ACGCTTTGGT GAAGCAGCCG
    CCAAACGAAA CGACTCCGGC GATAATTGTG TTCGGAGATT CAATCGTTGA TGCCGGAAAT
    AATGACGACA TTATGACAAC GTTGGCTAGA TGCAATTATC CTCCTTACGG CATCGATTTT
    GACGGTGGAA TTCCTACCGG AAGATTTTGC AACGGCAAAG TTGCGACTGA TTTTATAGCG
    GGAAAATTCG GAATTAAACC AAGTATACCG GCATATCGAA ATCCAAATTT GAAACCAGAA
    GATCTCTTAA CCGGTGTAAC ATTTGCGTCT GGTGGTGCTG GTTACGTTCC CTTCACAACC
    CAATTATCGA CGTACCTTTT TATATATAAA CCATTGTTGT TCTTAAAGGG AGGAATAGCT
    TTATCGCAGC AACTGAAGTT ATTCGAAGAG TATGTAGAGA AGATGAAGAA AATGGTTGGG
    GAAGAAAGGA CAAAGCTCAT AATTAAAAAC AGTTTGTTCA TGGTTATATG TGGCAGTAAT
    GATATAACCA ACACCTACTT TGGTCTTCCT TCTGTTCAAC AACAATACGA CGTCGCTTCC
    TTTACCACTC TTATGGCCGA CAATGCTCGA TCTTTTGCTC AAAAATTACA TGAATATGGA
    GCGAGGAGAA TACAAGTATT CGGTGCACCA CCAGTAGGAT GTGTCCCATC ACAGAGAACC
    TTAGCAGGAG GGCCAACAAG AAATTGTGTT GTTAGGTTCA ATGATGCAAC AAAGCTCTAC
    AATGTTAAGC TCGCTGCAAA TTTGGGTTCT TTGTCAAGAA CTTTAGGAGA CAAGACAATA
    ATCTACGTTG ACATATATGA TTCTCTCCTT GATATCATTC TTGACCCTCG ACAATATGGA
    TTTAAGGTGG TTGATAAAGG ATGTTGTGGA ACTGGACTAA TTGAAGTTGC TCTTTTATGC
    AACAACTTTG CAGCTGATGT ATGTCCAAAT AGAGATGAAT ATGTGTTTTG GGATAGTTTC
    CATCCGACTG AGAAGACTTA CAGAATTATG GCCACAAAAT ATTTTGAGAG ATATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_565121.1
    CDS1..1140
    Translation

    Target ORF information:

    RefSeq Version NM_106239.2
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana GDSL-like Lipase/Acylhydrolase superfamily protein (AT1G75890), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_106239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAACGAA ACAGTATAAA TATTCATCAC GTTACTTCTT TTTCTTCGAG TCCGTTTTGG 
    TGTGTTTTCT TTCTCGTGTT GTTATGTAAG ACGTCCACCA ACGCTTTGGT GAAGCAGCCG
    CCAAACGAAA CGACTCCGGC GATAATTGTG TTCGGAGATT CAATCGTTGA TGCCGGAAAT
    AATGACGACA TTATGACAAC GTTGGCTAGA TGCAATTATC CTCCTTACGG CATCGATTTT
    GACGGTGGAA TTCCTACCGG AAGATTTTGC AACGGCAAAG TTGCGACTGA TTTTATAGCG
    GGAAAATTCG GAATTAAACC AAGTATACCG GCATATCGAA ATCCAAATTT GAAACCAGAA
    GATCTCTTAA CCGGTGTAAC ATTTGCGTCT GGTGGTGCTG GTTACGTTCC CTTCACAACC
    CAATTATCGA CGTACCTTTT TATATATAAA CCATTGTTGT TCTTAAAGGG AGGAATAGCT
    TTATCGCAGC AACTGAAGTT ATTCGAAGAG TATGTAGAGA AGATGAAGAA AATGGTTGGG
    GAAGAAAGGA CAAAGCTCAT AATTAAAAAC AGTTTGTTCA TGGTTATATG TGGCAGTAAT
    GATATAACCA ACACCTACTT TGGTCTTCCT TCTGTTCAAC AACAATACGA CGTCGCTTCC
    TTTACCACTC TTATGGCCGA CAATGCTCGA TCTTTTGCTC AAAAATTACA TGAATATGGA
    GCGAGGAGAA TACAAGTATT CGGTGCACCA CCAGTAGGAT GTGTCCCATC ACAGAGAACC
    TTAGCAGGAG GGCCAACAAG AAATTGTGTT GTTAGGTTCA ATGATGCAAC AAAGCTCTAC
    AATGTTAAGC TCGCTGCAAA TTTGGGTTCT TTGTCAAGAA CTTTAGGAGA CAAGACAATA
    ATCTACGTTG ACATATATGA TTCTCTCCTT GATATCATTC TTGACCCTCG ACAATATGGA
    TTTAAGGTGG TTGATAAAGG ATGTTGTGGA ACTGGACTAA TTGAAGTTGC TCTTTTATGC
    AACAACTTTG CAGCTGATGT ATGTCCAAAT AGAGATGAAT ATGTGTTTTG GGATAGTTTC
    CATCCGACTG AGAAGACTTA CAGAATTATG GCCACAAAAT ATTTTGAGAG ATATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb34984
    Clone ID Related Accession (Same CDS sequence) NM_001334701.1
    Accession Version NM_001334701.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product GDSL-like Lipase/Acylhydrolase superfamily protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTTAAATT TTCACATGTC GATCCAAGCT CTACATATAT ATATTTACTC ATCATTCCAA 
    AAGCTTCCAA AGAAACAAAG AGTAACAATG AAACGAAACA GTATAAATAT TCATCACGTT
    ACTTCTTTTT CTTCGAGTCC GTTTTGGTGT GTTTTCTTTC TCGTGTTGTT ATGTAAGACG
    TCCACCAACG CTTTGGTGAA GCAGCCGCCA AACGAAACGA CTCCGGCGAT AATTGTGTTC
    GGAGATTCAA TCGTTGATGC CGGAAATAAT GACGACATTA TGACAACGTT GGCTAGATGC
    AATTATCCTC CTTACGGCAT CGATTTTGAC GGTGGAATTC CTACCGGAAG ATTTTGCAAC
    GGCAAAGTTG CGACTGATTT TATAGCGGGA AAATTCGGAA TTAAACCAAG TATACCGGCA
    TATCGAAATC CAAATTTGAA ACCAGAAGAT CTCTTAACCG GTGTAACATT TGCGTCTGGT
    GGTGCTGGTT ACGTTCCCTT CACAACCCAA TTATCGGGAG GAATAGCTTT ATCGCAGCAA
    CTGAAGTTAT TCGAAGAGTA TGTAGAGAAG ATGAAGAAAA TGGTTGGGGA AGAAAGGACA
    AAGCTCATAA TTAAAAACAG TTTGTTCATG GTTATATGTG GCAGTAATGA TATAACCAAC
    ACCTACTTTG GTCTTCCTTC TGTTCAACAA CAATACGACG TCGCTTCCTT TACCACTCTT
    ATGGCCGACA ATGCTCGATC TTTTGCTCAA AAATTACATG AATATGGAGC GAGGAGAATA
    CAAGTATTCG GTGCACCACC AGTAGGATGT GTCCCATCAC AGAGAACCTT AGCAGGAGGG
    CCAACAAGAA ATTGTGTTGT TAGGTTCAAT GATGCAACAA AGCTCTACAA TGTTAAGCTC
    GCTGCAAATT TGGGTTCTTT GTCAAGAACT TTAGGAGACA AGACAATAAT CTACGTTGAC
    ATATATGATT CTCTCCTTGA TATCATTCTT GACCCTCGAC AATATGGATT TAAGGTGGTT
    GATAAAGGAT GTTGTGGAAC TGGACTAATT GAAGTTGCTC TTTTATGCAA CAACTTTGCA
    GCTGATGTAT GTCCAAATAG AGATGAATAT GTGTTTTGGG ATAGTTTCCA TCCGACTGAG
    AAGACTTACA GAATTATGGC CACAAAATAT TTTGAGAGAT ATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001322026.1
    CDS21..1208
    Translation

    Target ORF information:

    RefSeq Version NM_001334701.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana GDSL-like Lipase/Acylhydrolase superfamily protein (AT1G75890), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001334701.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTTAAATT TTCACATGTC GATCCAAGCT CTACATATAT ATATTTACTC ATCATTCCAA 
    AAGCTTCCAA AGAAACAAAG AGTAACAATG AAACGAAACA GTATAAATAT TCATCACGTT
    ACTTCTTTTT CTTCGAGTCC GTTTTGGTGT GTTTTCTTTC TCGTGTTGTT ATGTAAGACG
    TCCACCAACG CTTTGGTGAA GCAGCCGCCA AACGAAACGA CTCCGGCGAT AATTGTGTTC
    GGAGATTCAA TCGTTGATGC CGGAAATAAT GACGACATTA TGACAACGTT GGCTAGATGC
    AATTATCCTC CTTACGGCAT CGATTTTGAC GGTGGAATTC CTACCGGAAG ATTTTGCAAC
    GGCAAAGTTG CGACTGATTT TATAGCGGGA AAATTCGGAA TTAAACCAAG TATACCGGCA
    TATCGAAATC CAAATTTGAA ACCAGAAGAT CTCTTAACCG GTGTAACATT TGCGTCTGGT
    GGTGCTGGTT ACGTTCCCTT CACAACCCAA TTATCGGGAG GAATAGCTTT ATCGCAGCAA
    CTGAAGTTAT TCGAAGAGTA TGTAGAGAAG ATGAAGAAAA TGGTTGGGGA AGAAAGGACA
    AAGCTCATAA TTAAAAACAG TTTGTTCATG GTTATATGTG GCAGTAATGA TATAACCAAC
    ACCTACTTTG GTCTTCCTTC TGTTCAACAA CAATACGACG TCGCTTCCTT TACCACTCTT
    ATGGCCGACA ATGCTCGATC TTTTGCTCAA AAATTACATG AATATGGAGC GAGGAGAATA
    CAAGTATTCG GTGCACCACC AGTAGGATGT GTCCCATCAC AGAGAACCTT AGCAGGAGGG
    CCAACAAGAA ATTGTGTTGT TAGGTTCAAT GATGCAACAA AGCTCTACAA TGTTAAGCTC
    GCTGCAAATT TGGGTTCTTT GTCAAGAACT TTAGGAGACA AGACAATAAT CTACGTTGAC
    ATATATGATT CTCTCCTTGA TATCATTCTT GACCCTCGAC AATATGGATT TAAGGTGGTT
    GATAAAGGAT GTTGTGGAAC TGGACTAATT GAAGTTGCTC TTTTATGCAA CAACTTTGCA
    GCTGATGTAT GTCCAAATAG AGATGAATAT GTGTTTTGGG ATAGTTTCCA TCCGACTGAG
    AAGACTTACA GAATTATGGC CACAAAATAT TTTGAGAGAT ATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
    Nature408(6814)816-20(2000 Dec)
    Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW