CALS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CALS1
Entrez Gene ID	837059
Full Name	callose synthase 1
Synonyms	ATGSL06,ATGSL6,F3F20.1,F3F20_1,GLUCAN SYNTHASE-LIKE 6,GSL06,GSL6,callose synthase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001331555.1	NP_001322482.1	callose synthase 1
NM_100436.5	NP_563743.2	callose synthase 1
NM_001331556.1	NP_001322483.1	callose synthase 1
NM_001197984.1	NP_001184913.1	callose synthase 1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	bgs3	NP_594766.1
Arabidopsis thaliana (thale cress)	GSL07	NP_172136.2
Arabidopsis thaliana (thale cress)	CALS5	NP_849953.2
Arabidopsis thaliana (thale cress)	GSL8	NP_850271.5
Arabidopsis thaliana (thale cress)	GSL04	NP_188075.2
Arabidopsis thaliana (thale cress)	GSL12	NP_196804.6
Arabidopsis thaliana (thale cress)	GSL03	NP_001189653.1
Arabidopsis thaliana (thale cress)	GSL10	NP_187372.5
Arabidopsis thaliana (thale cress)	GSL11	NP_191469.3
Arabidopsis thaliana (thale cress)	CALS1	NP_563743.2
Arabidopsis thaliana (thale cress)	GSL09	NP_198503.3
Oryza sativa (rice)	Os02g0832500	NP_001048628.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CALS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CALS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb22755	NM_100436.5 Latest version!	Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb22755	NM_001331555.1 Latest version!	Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb22755	NM_001331556.1 Latest version!	Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb29688	NM_001197984.1 Latest version!	Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CALS1 ( NM_001331555.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322482.1 Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.

Price & Availability↑

CloneID	OAb22755
Clone ID Related Accession (Same CDS sequence)	NM_001331555.1 , NM_100436.5 , NM_001331556.1
Accession Version	NM_001331555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	callose synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCTCAAA GAAGGGAACC TGATCCGCCT CCACCGCAGA GGCGGATTCT GCGGACTCAG 
ACTGTTGGTA GTCTCGGAGA GGCTATGCTT GATAGTGAAG TTGTTCCCTC TTCTTTAGTT 
GAGATTGCTC CGATTCTACG TGTGGCTAAT GAAGTTGAAG CTAGTAATCC ACGAGTTGCT 
TACCTATGTC GTTTCTACGC ATTTGAGAAG GCGCATAGGC TTGATCCAAC TTCAAGTGGT 
CGTGGTGTTC GCCAGTTTAA GACCGCGCTT CTTCAACGAT TAGAAAGGGA GAATGAAACA 
ACTTTGGCAG GAAGGCAAAA GAGTGATGCA CGTGAAATGC AGAGTTTCTA TCAACACTAC 
TACAAGAAAT ACATCCAAGC TTTGCTGAAT GCTGCGGATA AAGCTGACCG TGCTCAACTT 
ACAAAGGCCT ATCAAACTGC TGCCGTTCTC TTTGAAGTCT TGAAGGCTGT TAATCAAACA 
GAAGATGTGG AAGTGGCTGA TGAGATTTTG GAAACGCACA ATAAGGTGGA AGAGAAGACC 
CAGATTTACG TGCCTTACAA TATACTTCCC CTTGACCCTG ATAGTCAGAA TCAGGCAATA 
ATGAGATTGC CTGAGATACA AGCTGCTGTA GCCGCTCTTA GAAATACAAG GGGATTACCA 
TGGACAGCAG GTCACAAGAA GAAACTCGAT GAAGACATTC TAGACTGGCT TCAGTCTATG 
TTTGGTTTTC AGAAAGATAA TGTTTTGAAT CAAAGAGAGC ACTTGATCTT GTTACTTGCT 
AATGTCCACA TCCGACAATT TCCAAAACCT GACCAACAAC CAAAGTTGGA TGATCGTGCC 
TTGACCATAG TGATGAAGAA ACTTTTCAGA AACTACAAAA AGTGGTGCAA ATACTTGGGT 
CGGAAAAGCA GCCTTTGGTT GCCTACTATT CAGCAAGAGG TACAGCAGCG TAAACTGCTC 
TACATGGGAC TTTATCTATT AATATGGGGA GAAGCTGCAA ACCTGAGATT CATGCCAGAA 
TGCCTCTGTT ATATATATCA CCATATGGCT TTCGAGCTTT ATGGTATGTT GGCCGGTAGT 
GTTAGTCCCA TGACAGGGGA GCATGTAAAG CCAGCGTATG GTGGTGAAGA TGAAGCTTTC 
CTGCAGAAAG TTGTGACTCC TATATACCAG ACAATATCAA AGGAAGCAAA GAGAAGTAGA 
GGAGGAAAGT CAAAGCATTC TGTGTGGAGA AACTATGATG ACTTGAATGA ATATTTTTGG 
TCAATTCGAT GTTTTCGCTT AGGGTGGCCA ATGCGGGCTG ATGCTGATTT CTTTTGTCAG 
ACAGCTGAAG AACTTCGACT TGAGAGAAGT GAGATAAAAT CAAACAGTGG AGATCGGTGG 
ATGGGCAAAG TCAATTTTGT GGAGATACGC TCGTTCTGGC ATATATTTAG AAGTTTTGAT 
AGATTGTGGA GCTTTTATAT CCTGTGTTTG CAGGCAATGA TTGTTATTGC ATGGAATGGA 
TCCGGAGAGT TAAGCGCCAT TTTTCAGGGT GATGTCTTCC TAAAGGTTTT GAGCGTCTTC 
ATTACAGCAG CTATACTAAA GCTTGCACAA GCTGTCCTTG ACATAGCCCT CAGCTGGAAG 
GCAAGACATA GTATGTCACT CTATGTGAAA CTCAGATACG TCATGAAGGT AGGTGCGGCT 
GCAGTATGGG TTGTAGTCAT GGCAGTTACT TATGCGTACA GCTGGAAAAA CGCCTCCGGT 
TTTTCTCAGA CCATAAAGAA TTGGTTTGGG GGACACAGCC ATAATTCACC TTCTCTTTTC 
ATAGTGGCAA TTCTTATCTA CTTATCCCCG AACATGCTTT CTGCGTTGCT GTTTTTGTTT 
CCATTCATTC GGCGCTATCT TGAAAGGTCT GACTATAAGA TAATGATGCT GATGATGTGG 
TGGTCTCAGC CTCGTCTGTA TATAGGAAGG GGTATGCATG AGAGCGCTTT GTCACTTTTC 
AAGTACACAA TGTTTTGGAT CGTTCTGCTG ATTTCAAAGC TGGCATTCAG TTATTACGCT 
GAGATTAAGC CCCTTGTTGG TCCAACCAAA GATATAATGC GAATCCATAT ATCCGTCTAC 
AGCTGGCATG AGTTTTTCCC TCACGCCAAG AATAATTTAG GAGTCGTCAT CGCGCTCTGG 
TCACCAGTTA TTCTCGTTTA TTTCATGGAC ACTCAAATAT GGTATGCTAT AGTTTCAACT 
CTGGTCGGAG GTTTAAATGG AGCTTTCCGC CGTCTTGGAG AGATCCGAAC ACTTGGAATG 
TTGAGGTCGA GATTTCAGTC CATACCAGGG GCCTTTAATG ATTGCTTGGT ACCACAAGAT 
AACAGCGATG ATACAAAGAA AAAGAGATTT AGGGCGACCT TCTCTCGCAA GTTTGATCAG 
CTCCCATCTA GTAAAGACAA GGAAGCAGCG CGGTTTGCTC AGATGTGGAA CAAAATAATC 
AGTAGTTTCC GAGAAGAGGA CTTGATTAGT GATAGGGAAA TGGAACTATT GCTTGTGCCA 
TATTGGTCTG ACCCTGATTT GGATCTTATC CGGTGGCCAC CTTTTCTATT GGCTAGTAAG 
ATCCCAATAG CTTTGGATAT GGCTAAAGAC AGCAATGGAA AAGACCGTGA ACTGAAAAAG 
AGATTAGCTG TTGACAGCTA CATGACTTGT GCAGTTAGAG AGTGCTATGC CTCTTTTAAG 
AATCTCATCA ATTATTTGGT GGTCGGAGAA CGAGAAGGAC AGGTCATAAA CGACATTTTC 
TCCAAGATTG ATGAACATAT TGAGAAGGAA ACGCTGATAA CAGAATTGAA CCTGAGTGCT 
CTTCCTGATC TGTATGGCCA GTTCGTTCGG CTAATCGAAT ATTTGCTAGA AAACAGGGAG 
GAGGACAAGG ATCAAATTGT AATTGTCTTG CTGAACATGT TAGAATTGGT GACTAGAGAC 
ATCATGGAAG AAGAAGTCCC AAGCTTACTA GAGACTGCCC ACAATGGATC TTATGTCAAG 
TATGACGTCA TGACCCCTCT TCATCAACAG CGCAAATATT TCAGTCAACT TCGATTCCCA 
GTTTATTCTC AAACGGAAGC TTGGAAAGAA AAAATTAAGA GGCTTCATCT TTTGCTTACT 
GTCAAGGAGT CAGCTATGGA TGTTCCATCC AACTTGGAAG CTCGAAGGCG TCTTACTTTC 
TTTTCAAATT CACTATTCAT GGACATGCCT CCTGCACCCA AGATCCGCAA CATGCTTTCC 
TTCTCGGTCC TTACACCATA CTTTTCAGAG GATGTTCTTT TCTCCATATT TGGCTTAGAA 
CAGCAAAATG AAGATGGAGT TTCTATACTC TTTTACTTGC AGAAGATCTT CCCAGATGAA 
TGGACAAACT TTCTGGAACG AGTTAAATGT GGCAACGAAG AAGAACTAAG AGCAAGAGAG 
GATTTGGAAG AGGAGCTTCG TCTTTGGGCA TCTTACAGAG GCCAAACTCT GACTAAAACT 
GTGCGAGGAA TGATGTATTA CCGAAAGGCT TTGGAACTTC AGGCCTTCCT TGATATGGCA 
AAGGATGAAG AATTATTAAA AGGCTACAAG GCTCTCGAGT TAACCAGTGA AGAAGCATCA 
AAGAGCGGAG GGTCACTTTG GGCGCAGTGT CAAGCACTTG CAGATATGAA GTTCACCTTT 
GTGGTTTCTT GTCAACAATA CAGTATTCAT AAGAGATCTG GCGACCAACG TGCTAAAGAC 
ATATTGAGAC TTATGACGAC GTATCCGTCT ATTCGTGTCG CCTATATCGA TGAGGTAGAG 
CAAACACATA AAGAAAGTTA TAAGGGGACA GAGGAGAAAA TTTATTACTC AGCGCTTGTA 
AAAGCTGCTC CACAGACCAA ACCAATGGAT TCTTCTGAAT CTGTTCAAAC ACTAGATCAG 
CTCATTTATC GGATTAAGCT TCCGGGACCA GCTATACTAG GAGAGGGAAA GCCGGAAAAT 
CAGAACCATG CTATCATATT TACCCGTGGA GAAGGGTTGC AGACGATAGA CATGAATCAG 
GACAACTACA TGGAGGAAGC TTTCAAAATG AGGAACTTGC TGCAAGAGTT TCTTGAAAAG 
CATGGAGGCG TAAGATGTCC TACGATTCTT GGTCTTAGAG AGCATATTTT CACTGGAAGT 
GTGTCTTCTC TTGCATGGTT TATGTCAAAT CAAGAGAACA GTTTTGTAAC GATTGGGCAA 
AGAGTGCTAG CTAGTCCCTT GAAAGTACGA TTCCATTACG GACATCCAGA TATTTTTGAT 
CGTCTGTTTC ACCTTACCAG AGGTGGTATC TGCAAAGCTT CTAAAGTCAT CAATCTTAGT 
GAAGACATAT TTGCAGGTTT TAATTCTACT CTGCGTGAAG GAAATGTGAC TCATCATGAA 
TATATACAAG TTGGTAAAGG GAGAGATGTG GGCCTCAACC AGATCTCAAT GTTTGAGGCA 
AAAATCGCCA ATGGAAATGG CGAGCAAACT TTGAGTCGCG ACCTTTATAG GCTAGGACAC 
CGATTTGATT TCTTCCGGAT GCTGTCTTGT TATTTCACCA CAATTGGGTT CTACTTCAGT 
ACCATGTTAA CCGTGCTTAC GGTGTATGTG TTTCTTTATG GACGGTTATA TCTAGTACTT 
AGTGGGCTTG AAGAAGGATT GAGTAGCCAA CGAGCTTTCC GTAACAATAA GCCTCTTGAG 
GCAGCTCTTG CTTCTCAATC ATTTGTGCAG ATTGGTTTTT TAATGGCTTT GCCTATGATG 
ATGGAGATTG GACTTGAGAG GGGGTTCCAC AATGCATTGA TTGAGTTTGT GCTGATGCAG 
TTACAACTTG CTTCCGTCTT TTTCACGTTT CAGCTCGGGA CAAAAACACA TTACTACGGA 
AGGACATTGT TTCATGGAGG CGCAGAGTAT AGAGGAACTG GTCGAGGGTT TGTGGTCTTC 
CACGCGAAAT TTGCTGAGAA CTATAGGTTC TATTCCCGTA GTCACTTTGT GAAGGGCATT 
GAACTTATGA TTCTTCTACT GGTATATCAG ATCTTTGGTC AATCATATAG GGGTGTGGTT 
ACTTATATTC TGATCACTGT TTCGATATGG TTCATGGTGG TGACATGGCT CTTTGCTCCT 
TTCCTTTTCA ATCCGTCTGG TTTTGAGTGG CAAAAGATTG TTGATGACTG GACAGACTGG 
AACAAATGGA TTTATAACCG AGGAGGAATT GGTGTCCCTC CTGAGAAGAG TTGGGAATCT 
TGGTGGGAGA AAGAGTTAGA ACATCTTAGA CATTCAGGGG TTCGTGGTAT CACCCTTGAG 
ATCTTTTTGG CCTTGCGCTT CTTCATCTTT CAATATGGAC TTGTGTATCA TCTCAGTACC 
TTCAAAGGAA AAAATCAGAG CTTTTGGGTT TATGGAGCGT CATGGTTTGT GATCTTATTC 
ATTCTACTGA TTGTGAAGGG TTTGGGCGTT GGAAGGCGAA GATTCAGCAC GAATTTCCAG 
CTTCTATTCA GAATCATAAA GGGTCTTGTG TTCCTCACAT TTGTGGCAAT TCTCATAACT 
TTCCTCGCAC TTCCTCTGAT AACAATCAAA GACTTATTCA TCTGCATGCT TGCCTTCATG 
CCAACTGGAT GGGGAATGCT TCTCATTGCA CAAGCGTGTA AGCCTCTGAT CCAACAGCTA 
GGGATATGGT CATCAGTTAG GACGCTAGCT CGAGGCTACG AGATCGTGAT GGGCTTGCTT 
CTGTTCACAC CAGTTGCGTT TTTGGCCTGG TTTCCTTTTG TGTCTGAGTT CCAGACAAGA 
ATGCTCTTTA ACCAAGCCTT TAGTAGAGGT CTACAAATCT CTCGTATTCT TGGTGGTCAA 
AGAAAAGACC GGTCTTCAAA AAACAAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322482.1
CDS	296..6148
Translation	MAQRREPDPPPPQRRILRTQTVGSLGEAMLDSEVVPSSLVEIAPILRVANEVEASNPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALLQRLERENETTLAGRQKSDAREMQSFYQHYYKKYIQALLNAADKADRAQLTKAYQTAAVLFEVLKAVNQTEDVEVADEILETHNKVEEKTQIYVPYNILPLDPDSQNQAIMRLPEIQAAVAALRNTRGLPWTAGHKKKLDEDILDWLQSMFGFQKDNVLNQREHLILLLANVHIRQFPKPDQQPKLDDRALTIVMKKLFRNYKKWCKYLGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGSVSPMTGEHVKPAYGGEDEAFLQKVVTPIYQTISKEAKRSRGGKSKHSVWRNYDDLNEYFWSIRCFRLGWPMRADADFFCQTAEELRLERSEIKSNSGDRWMGKVNFVEIRSFWHIFRSFDRLWSFYILCLQAMIVIAWNGSGELSAIFQGDVFLKVLSVFITAAILKLAQAVLDIALSWKARHSMSLYVKLRYVMKVGAAAVWVVVMAVTYAYSWKNASGFSQTIKNWFGGHSHNSPSLFIVAILIYLSPNMLSALLFLFPFIRRYLERSDYKIMMLMMWWSQPRLYIGRGMHESALSLFKYTMFWIVLLISKLAFSYYAEIKPLVGPTKDIMRIHISVYSWHEFFPHAKNNLGVVIALWSPVILVYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSIPGAFNDCLVPQDNSDDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISSFREEDLISDREMELLLVPYWSDPDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELKKRLAVDSYMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALPDLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLERVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEELLKGYKALELTSEEASKSGGSLWAQCQALADMKFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYKGTEEKIYYSALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE

Target ORF information:

RefSeq Version	NM_001331555.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331555.1

ORF Insert Sequence:

ATGGCTCAAA GAAGGGAACC TGATCCGCCT CCACCGCAGA GGCGGATTCT GCGGACTCAG 
ACTGTTGGTA GTCTCGGAGA GGCTATGCTT GATAGTGAAG TTGTTCCCTC TTCTTTAGTT 
GAGATTGCTC CGATTCTACG TGTGGCTAAT GAAGTTGAAG CTAGTAATCC ACGAGTTGCT 
TACCTATGTC GTTTCTACGC ATTTGAGAAG GCGCATAGGC TTGATCCAAC TTCAAGTGGT 
CGTGGTGTTC GCCAGTTTAA GACCGCGCTT CTTCAACGAT TAGAAAGGGA GAATGAAACA 
ACTTTGGCAG GAAGGCAAAA GAGTGATGCA CGTGAAATGC AGAGTTTCTA TCAACACTAC 
TACAAGAAAT ACATCCAAGC TTTGCTGAAT GCTGCGGATA AAGCTGACCG TGCTCAACTT 
ACAAAGGCCT ATCAAACTGC TGCCGTTCTC TTTGAAGTCT TGAAGGCTGT TAATCAAACA 
GAAGATGTGG AAGTGGCTGA TGAGATTTTG GAAACGCACA ATAAGGTGGA AGAGAAGACC 
CAGATTTACG TGCCTTACAA TATACTTCCC CTTGACCCTG ATAGTCAGAA TCAGGCAATA 
ATGAGATTGC CTGAGATACA AGCTGCTGTA GCCGCTCTTA GAAATACAAG GGGATTACCA 
TGGACAGCAG GTCACAAGAA GAAACTCGAT GAAGACATTC TAGACTGGCT TCAGTCTATG 
TTTGGTTTTC AGAAAGATAA TGTTTTGAAT CAAAGAGAGC ACTTGATCTT GTTACTTGCT 
AATGTCCACA TCCGACAATT TCCAAAACCT GACCAACAAC CAAAGTTGGA TGATCGTGCC 
TTGACCATAG TGATGAAGAA ACTTTTCAGA AACTACAAAA AGTGGTGCAA ATACTTGGGT 
CGGAAAAGCA GCCTTTGGTT GCCTACTATT CAGCAAGAGG TACAGCAGCG TAAACTGCTC 
TACATGGGAC TTTATCTATT AATATGGGGA GAAGCTGCAA ACCTGAGATT CATGCCAGAA 
TGCCTCTGTT ATATATATCA CCATATGGCT TTCGAGCTTT ATGGTATGTT GGCCGGTAGT 
GTTAGTCCCA TGACAGGGGA GCATGTAAAG CCAGCGTATG GTGGTGAAGA TGAAGCTTTC 
CTGCAGAAAG TTGTGACTCC TATATACCAG ACAATATCAA AGGAAGCAAA GAGAAGTAGA 
GGAGGAAAGT CAAAGCATTC TGTGTGGAGA AACTATGATG ACTTGAATGA ATATTTTTGG 
TCAATTCGAT GTTTTCGCTT AGGGTGGCCA ATGCGGGCTG ATGCTGATTT CTTTTGTCAG 
ACAGCTGAAG AACTTCGACT TGAGAGAAGT GAGATAAAAT CAAACAGTGG AGATCGGTGG 
ATGGGCAAAG TCAATTTTGT GGAGATACGC TCGTTCTGGC ATATATTTAG AAGTTTTGAT 
AGATTGTGGA GCTTTTATAT CCTGTGTTTG CAGGCAATGA TTGTTATTGC ATGGAATGGA 
TCCGGAGAGT TAAGCGCCAT TTTTCAGGGT GATGTCTTCC TAAAGGTTTT GAGCGTCTTC 
ATTACAGCAG CTATACTAAA GCTTGCACAA GCTGTCCTTG ACATAGCCCT CAGCTGGAAG 
GCAAGACATA GTATGTCACT CTATGTGAAA CTCAGATACG TCATGAAGGT AGGTGCGGCT 
GCAGTATGGG TTGTAGTCAT GGCAGTTACT TATGCGTACA GCTGGAAAAA CGCCTCCGGT 
TTTTCTCAGA CCATAAAGAA TTGGTTTGGG GGACACAGCC ATAATTCACC TTCTCTTTTC 
ATAGTGGCAA TTCTTATCTA CTTATCCCCG AACATGCTTT CTGCGTTGCT GTTTTTGTTT 
CCATTCATTC GGCGCTATCT TGAAAGGTCT GACTATAAGA TAATGATGCT GATGATGTGG 
TGGTCTCAGC CTCGTCTGTA TATAGGAAGG GGTATGCATG AGAGCGCTTT GTCACTTTTC 
AAGTACACAA TGTTTTGGAT CGTTCTGCTG ATTTCAAAGC TGGCATTCAG TTATTACGCT 
GAGATTAAGC CCCTTGTTGG TCCAACCAAA GATATAATGC GAATCCATAT ATCCGTCTAC 
AGCTGGCATG AGTTTTTCCC TCACGCCAAG AATAATTTAG GAGTCGTCAT CGCGCTCTGG 
TCACCAGTTA TTCTCGTTTA TTTCATGGAC ACTCAAATAT GGTATGCTAT AGTTTCAACT 
CTGGTCGGAG GTTTAAATGG AGCTTTCCGC CGTCTTGGAG AGATCCGAAC ACTTGGAATG 
TTGAGGTCGA GATTTCAGTC CATACCAGGG GCCTTTAATG ATTGCTTGGT ACCACAAGAT 
AACAGCGATG ATACAAAGAA AAAGAGATTT AGGGCGACCT TCTCTCGCAA GTTTGATCAG 
CTCCCATCTA GTAAAGACAA GGAAGCAGCG CGGTTTGCTC AGATGTGGAA CAAAATAATC 
AGTAGTTTCC GAGAAGAGGA CTTGATTAGT GATAGGGAAA TGGAACTATT GCTTGTGCCA 
TATTGGTCTG ACCCTGATTT GGATCTTATC CGGTGGCCAC CTTTTCTATT GGCTAGTAAG 
ATCCCAATAG CTTTGGATAT GGCTAAAGAC AGCAATGGAA AAGACCGTGA ACTGAAAAAG 
AGATTAGCTG TTGACAGCTA CATGACTTGT GCAGTTAGAG AGTGCTATGC CTCTTTTAAG 
AATCTCATCA ATTATTTGGT GGTCGGAGAA CGAGAAGGAC AGGTCATAAA CGACATTTTC 
TCCAAGATTG ATGAACATAT TGAGAAGGAA ACGCTGATAA CAGAATTGAA CCTGAGTGCT 
CTTCCTGATC TGTATGGCCA GTTCGTTCGG CTAATCGAAT ATTTGCTAGA AAACAGGGAG 
GAGGACAAGG ATCAAATTGT AATTGTCTTG CTGAACATGT TAGAATTGGT GACTAGAGAC 
ATCATGGAAG AAGAAGTCCC AAGCTTACTA GAGACTGCCC ACAATGGATC TTATGTCAAG 
TATGACGTCA TGACCCCTCT TCATCAACAG CGCAAATATT TCAGTCAACT TCGATTCCCA 
GTTTATTCTC AAACGGAAGC TTGGAAAGAA AAAATTAAGA GGCTTCATCT TTTGCTTACT 
GTCAAGGAGT CAGCTATGGA TGTTCCATCC AACTTGGAAG CTCGAAGGCG TCTTACTTTC 
TTTTCAAATT CACTATTCAT GGACATGCCT CCTGCACCCA AGATCCGCAA CATGCTTTCC 
TTCTCGGTCC TTACACCATA CTTTTCAGAG GATGTTCTTT TCTCCATATT TGGCTTAGAA 
CAGCAAAATG AAGATGGAGT TTCTATACTC TTTTACTTGC AGAAGATCTT CCCAGATGAA 
TGGACAAACT TTCTGGAACG AGTTAAATGT GGCAACGAAG AAGAACTAAG AGCAAGAGAG 
GATTTGGAAG AGGAGCTTCG TCTTTGGGCA TCTTACAGAG GCCAAACTCT GACTAAAACT 
GTGCGAGGAA TGATGTATTA CCGAAAGGCT TTGGAACTTC AGGCCTTCCT TGATATGGCA 
AAGGATGAAG AATTATTAAA AGGCTACAAG GCTCTCGAGT TAACCAGTGA AGAAGCATCA 
AAGAGCGGAG GGTCACTTTG GGCGCAGTGT CAAGCACTTG CAGATATGAA GTTCACCTTT 
GTGGTTTCTT GTCAACAATA CAGTATTCAT AAGAGATCTG GCGACCAACG TGCTAAAGAC 
ATATTGAGAC TTATGACGAC GTATCCGTCT ATTCGTGTCG CCTATATCGA TGAGGTAGAG 
CAAACACATA AAGAAAGTTA TAAGGGGACA GAGGAGAAAA TTTATTACTC AGCGCTTGTA 
AAAGCTGCTC CACAGACCAA ACCAATGGAT TCTTCTGAAT CTGTTCAAAC ACTAGATCAG 
CTCATTTATC GGATTAAGCT TCCGGGACCA GCTATACTAG GAGAGGGAAA GCCGGAAAAT 
CAGAACCATG CTATCATATT TACCCGTGGA GAAGGGTTGC AGACGATAGA CATGAATCAG 
GACAACTACA TGGAGGAAGC TTTCAAAATG AGGAACTTGC TGCAAGAGTT TCTTGAAAAG 
CATGGAGGCG TAAGATGTCC TACGATTCTT GGTCTTAGAG AGCATATTTT CACTGGAAGT 
GTGTCTTCTC TTGCATGGTT TATGTCAAAT CAAGAGAACA GTTTTGTAAC GATTGGGCAA 
AGAGTGCTAG CTAGTCCCTT GAAAGTACGA TTCCATTACG GACATCCAGA TATTTTTGAT 
CGTCTGTTTC ACCTTACCAG AGGTGGTATC TGCAAAGCTT CTAAAGTCAT CAATCTTAGT 
GAAGACATAT TTGCAGGTTT TAATTCTACT CTGCGTGAAG GAAATGTGAC TCATCATGAA 
TATATACAAG TTGGTAAAGG GAGAGATGTG GGCCTCAACC AGATCTCAAT GTTTGAGGCA 
AAAATCGCCA ATGGAAATGG CGAGCAAACT TTGAGTCGCG ACCTTTATAG GCTAGGACAC 
CGATTTGATT TCTTCCGGAT GCTGTCTTGT TATTTCACCA CAATTGGGTT CTACTTCAGT 
ACCATGTTAA CCGTGCTTAC GGTGTATGTG TTTCTTTATG GACGGTTATA TCTAGTACTT 
AGTGGGCTTG AAGAAGGATT GAGTAGCCAA CGAGCTTTCC GTAACAATAA GCCTCTTGAG 
GCAGCTCTTG CTTCTCAATC ATTTGTGCAG ATTGGTTTTT TAATGGCTTT GCCTATGATG 
ATGGAGATTG GACTTGAGAG GGGGTTCCAC AATGCATTGA TTGAGTTTGT GCTGATGCAG 
TTACAACTTG CTTCCGTCTT TTTCACGTTT CAGCTCGGGA CAAAAACACA TTACTACGGA 
AGGACATTGT TTCATGGAGG CGCAGAGTAT AGAGGAACTG GTCGAGGGTT TGTGGTCTTC 
CACGCGAAAT TTGCTGAGAA CTATAGGTTC TATTCCCGTA GTCACTTTGT GAAGGGCATT 
GAACTTATGA TTCTTCTACT GGTATATCAG ATCTTTGGTC AATCATATAG GGGTGTGGTT 
ACTTATATTC TGATCACTGT TTCGATATGG TTCATGGTGG TGACATGGCT CTTTGCTCCT 
TTCCTTTTCA ATCCGTCTGG TTTTGAGTGG CAAAAGATTG TTGATGACTG GACAGACTGG 
AACAAATGGA TTTATAACCG AGGAGGAATT GGTGTCCCTC CTGAGAAGAG TTGGGAATCT 
TGGTGGGAGA AAGAGTTAGA ACATCTTAGA CATTCAGGGG TTCGTGGTAT CACCCTTGAG 
ATCTTTTTGG CCTTGCGCTT CTTCATCTTT CAATATGGAC TTGTGTATCA TCTCAGTACC 
TTCAAAGGAA AAAATCAGAG CTTTTGGGTT TATGGAGCGT CATGGTTTGT GATCTTATTC 
ATTCTACTGA TTGTGAAGGG TTTGGGCGTT GGAAGGCGAA GATTCAGCAC GAATTTCCAG 
CTTCTATTCA GAATCATAAA GGGTCTTGTG TTCCTCACAT TTGTGGCAAT TCTCATAACT 
TTCCTCGCAC TTCCTCTGAT AACAATCAAA GACTTATTCA TCTGCATGCT TGCCTTCATG 
CCAACTGGAT GGGGAATGCT TCTCATTGCA CAAGCGTGTA AGCCTCTGAT CCAACAGCTA 
GGGATATGGT CATCAGTTAG GACGCTAGCT CGAGGCTACG AGATCGTGAT GGGCTTGCTT 
CTGTTCACAC CAGTTGCGTT TTTGGCCTGG TTTCCTTTTG TGTCTGAGTT CCAGACAAGA 
ATGCTCTTTA ACCAAGCCTT TAGTAGAGGT CTACAAATCT CTCGTATTCT TGGTGGTCAA 
AGAAAAGACC GGTCTTCAAA AAACAAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CALS1 ( NM_100436.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_563743.2 Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.

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CloneID	OAb22755
Clone ID Related Accession (Same CDS sequence)	NM_001331555.1 , NM_100436.5 , NM_001331556.1
Accession Version	NM_100436.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	callose synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_100436.4.

ATGGCTCAAA GAAGGGAACC TGATCCGCCT CCACCGCAGA GGCGGATTCT GCGGACTCAG 
ACTGTTGGTA GTCTCGGAGA GGCTATGCTT GATAGTGAAG TTGTTCCCTC TTCTTTAGTT 
GAGATTGCTC CGATTCTACG TGTGGCTAAT GAAGTTGAAG CTAGTAATCC ACGAGTTGCT 
TACCTATGTC GTTTCTACGC ATTTGAGAAG GCGCATAGGC TTGATCCAAC TTCAAGTGGT 
CGTGGTGTTC GCCAGTTTAA GACCGCGCTT CTTCAACGAT TAGAAAGGGA GAATGAAACA 
ACTTTGGCAG GAAGGCAAAA GAGTGATGCA CGTGAAATGC AGAGTTTCTA TCAACACTAC 
TACAAGAAAT ACATCCAAGC TTTGCTGAAT GCTGCGGATA AAGCTGACCG TGCTCAACTT 
ACAAAGGCCT ATCAAACTGC TGCCGTTCTC TTTGAAGTCT TGAAGGCTGT TAATCAAACA 
GAAGATGTGG AAGTGGCTGA TGAGATTTTG GAAACGCACA ATAAGGTGGA AGAGAAGACC 
CAGATTTACG TGCCTTACAA TATACTTCCC CTTGACCCTG ATAGTCAGAA TCAGGCAATA 
ATGAGATTGC CTGAGATACA AGCTGCTGTA GCCGCTCTTA GAAATACAAG GGGATTACCA 
TGGACAGCAG GTCACAAGAA GAAACTCGAT GAAGACATTC TAGACTGGCT TCAGTCTATG 
TTTGGTTTTC AGAAAGATAA TGTTTTGAAT CAAAGAGAGC ACTTGATCTT GTTACTTGCT 
AATGTCCACA TCCGACAATT TCCAAAACCT GACCAACAAC CAAAGTTGGA TGATCGTGCC 
TTGACCATAG TGATGAAGAA ACTTTTCAGA AACTACAAAA AGTGGTGCAA ATACTTGGGT 
CGGAAAAGCA GCCTTTGGTT GCCTACTATT CAGCAAGAGG TACAGCAGCG TAAACTGCTC 
TACATGGGAC TTTATCTATT AATATGGGGA GAAGCTGCAA ACCTGAGATT CATGCCAGAA 
TGCCTCTGTT ATATATATCA CCATATGGCT TTCGAGCTTT ATGGTATGTT GGCCGGTAGT 
GTTAGTCCCA TGACAGGGGA GCATGTAAAG CCAGCGTATG GTGGTGAAGA TGAAGCTTTC 
CTGCAGAAAG TTGTGACTCC TATATACCAG ACAATATCAA AGGAAGCAAA GAGAAGTAGA 
GGAGGAAAGT CAAAGCATTC TGTGTGGAGA AACTATGATG ACTTGAATGA ATATTTTTGG 
TCAATTCGAT GTTTTCGCTT AGGGTGGCCA ATGCGGGCTG ATGCTGATTT CTTTTGTCAG 
ACAGCTGAAG AACTTCGACT TGAGAGAAGT GAGATAAAAT CAAACAGTGG AGATCGGTGG 
ATGGGCAAAG TCAATTTTGT GGAGATACGC TCGTTCTGGC ATATATTTAG AAGTTTTGAT 
AGATTGTGGA GCTTTTATAT CCTGTGTTTG CAGGCAATGA TTGTTATTGC ATGGAATGGA 
TCCGGAGAGT TAAGCGCCAT TTTTCAGGGT GATGTCTTCC TAAAGGTTTT GAGCGTCTTC 
ATTACAGCAG CTATACTAAA GCTTGCACAA GCTGTCCTTG ACATAGCCCT CAGCTGGAAG 
GCAAGACATA GTATGTCACT CTATGTGAAA CTCAGATACG TCATGAAGGT AGGTGCGGCT 
GCAGTATGGG TTGTAGTCAT GGCAGTTACT TATGCGTACA GCTGGAAAAA CGCCTCCGGT 
TTTTCTCAGA CCATAAAGAA TTGGTTTGGG GGACACAGCC ATAATTCACC TTCTCTTTTC 
ATAGTGGCAA TTCTTATCTA CTTATCCCCG AACATGCTTT CTGCGTTGCT GTTTTTGTTT 
CCATTCATTC GGCGCTATCT TGAAAGGTCT GACTATAAGA TAATGATGCT GATGATGTGG 
TGGTCTCAGC CTCGTCTGTA TATAGGAAGG GGTATGCATG AGAGCGCTTT GTCACTTTTC 
AAGTACACAA TGTTTTGGAT CGTTCTGCTG ATTTCAAAGC TGGCATTCAG TTATTACGCT 
GAGATTAAGC CCCTTGTTGG TCCAACCAAA GATATAATGC GAATCCATAT ATCCGTCTAC 
AGCTGGCATG AGTTTTTCCC TCACGCCAAG AATAATTTAG GAGTCGTCAT CGCGCTCTGG 
TCACCAGTTA TTCTCGTTTA TTTCATGGAC ACTCAAATAT GGTATGCTAT AGTTTCAACT 
CTGGTCGGAG GTTTAAATGG AGCTTTCCGC CGTCTTGGAG AGATCCGAAC ACTTGGAATG 
TTGAGGTCGA GATTTCAGTC CATACCAGGG GCCTTTAATG ATTGCTTGGT ACCACAAGAT 
AACAGCGATG ATACAAAGAA AAAGAGATTT AGGGCGACCT TCTCTCGCAA GTTTGATCAG 
CTCCCATCTA GTAAAGACAA GGAAGCAGCG CGGTTTGCTC AGATGTGGAA CAAAATAATC 
AGTAGTTTCC GAGAAGAGGA CTTGATTAGT GATAGGGAAA TGGAACTATT GCTTGTGCCA 
TATTGGTCTG ACCCTGATTT GGATCTTATC CGGTGGCCAC CTTTTCTATT GGCTAGTAAG 
ATCCCAATAG CTTTGGATAT GGCTAAAGAC AGCAATGGAA AAGACCGTGA ACTGAAAAAG 
AGATTAGCTG TTGACAGCTA CATGACTTGT GCAGTTAGAG AGTGCTATGC CTCTTTTAAG 
AATCTCATCA ATTATTTGGT GGTCGGAGAA CGAGAAGGAC AGGTCATAAA CGACATTTTC 
TCCAAGATTG ATGAACATAT TGAGAAGGAA ACGCTGATAA CAGAATTGAA CCTGAGTGCT 
CTTCCTGATC TGTATGGCCA GTTCGTTCGG CTAATCGAAT ATTTGCTAGA AAACAGGGAG 
GAGGACAAGG ATCAAATTGT AATTGTCTTG CTGAACATGT TAGAATTGGT GACTAGAGAC 
ATCATGGAAG AAGAAGTCCC AAGCTTACTA GAGACTGCCC ACAATGGATC TTATGTCAAG 
TATGACGTCA TGACCCCTCT TCATCAACAG CGCAAATATT TCAGTCAACT TCGATTCCCA 
GTTTATTCTC AAACGGAAGC TTGGAAAGAA AAAATTAAGA GGCTTCATCT TTTGCTTACT 
GTCAAGGAGT CAGCTATGGA TGTTCCATCC AACTTGGAAG CTCGAAGGCG TCTTACTTTC 
TTTTCAAATT CACTATTCAT GGACATGCCT CCTGCACCCA AGATCCGCAA CATGCTTTCC 
TTCTCGGTCC TTACACCATA CTTTTCAGAG GATGTTCTTT TCTCCATATT TGGCTTAGAA 
CAGCAAAATG AAGATGGAGT TTCTATACTC TTTTACTTGC AGAAGATCTT CCCAGATGAA 
TGGACAAACT TTCTGGAACG AGTTAAATGT GGCAACGAAG AAGAACTAAG AGCAAGAGAG 
GATTTGGAAG AGGAGCTTCG TCTTTGGGCA TCTTACAGAG GCCAAACTCT GACTAAAACT 
GTGCGAGGAA TGATGTATTA CCGAAAGGCT TTGGAACTTC AGGCCTTCCT TGATATGGCA 
AAGGATGAAG AATTATTAAA AGGCTACAAG GCTCTCGAGT TAACCAGTGA AGAAGCATCA 
AAGAGCGGAG GGTCACTTTG GGCGCAGTGT CAAGCACTTG CAGATATGAA GTTCACCTTT 
GTGGTTTCTT GTCAACAATA CAGTATTCAT AAGAGATCTG GCGACCAACG TGCTAAAGAC 
ATATTGAGAC TTATGACGAC GTATCCGTCT ATTCGTGTCG CCTATATCGA TGAGGTAGAG 
CAAACACATA AAGAAAGTTA TAAGGGGACA GAGGAGAAAA TTTATTACTC AGCGCTTGTA 
AAAGCTGCTC CACAGACCAA ACCAATGGAT TCTTCTGAAT CTGTTCAAAC ACTAGATCAG 
CTCATTTATC GGATTAAGCT TCCGGGACCA GCTATACTAG GAGAGGGAAA GCCGGAAAAT 
CAGAACCATG CTATCATATT TACCCGTGGA GAAGGGTTGC AGACGATAGA CATGAATCAG 
GACAACTACA TGGAGGAAGC TTTCAAAATG AGGAACTTGC TGCAAGAGTT TCTTGAAAAG 
CATGGAGGCG TAAGATGTCC TACGATTCTT GGTCTTAGAG AGCATATTTT CACTGGAAGT 
GTGTCTTCTC TTGCATGGTT TATGTCAAAT CAAGAGAACA GTTTTGTAAC GATTGGGCAA 
AGAGTGCTAG CTAGTCCCTT GAAAGTACGA TTCCATTACG GACATCCAGA TATTTTTGAT 
CGTCTGTTTC ACCTTACCAG AGGTGGTATC TGCAAAGCTT CTAAAGTCAT CAATCTTAGT 
GAAGACATAT TTGCAGGTTT TAATTCTACT CTGCGTGAAG GAAATGTGAC TCATCATGAA 
TATATACAAG TTGGTAAAGG GAGAGATGTG GGCCTCAACC AGATCTCAAT GTTTGAGGCA 
AAAATCGCCA ATGGAAATGG CGAGCAAACT TTGAGTCGCG ACCTTTATAG GCTAGGACAC 
CGATTTGATT TCTTCCGGAT GCTGTCTTGT TATTTCACCA CAATTGGGTT CTACTTCAGT 
ACCATGTTAA CCGTGCTTAC GGTGTATGTG TTTCTTTATG GACGGTTATA TCTAGTACTT 
AGTGGGCTTG AAGAAGGATT GAGTAGCCAA CGAGCTTTCC GTAACAATAA GCCTCTTGAG 
GCAGCTCTTG CTTCTCAATC ATTTGTGCAG ATTGGTTTTT TAATGGCTTT GCCTATGATG 
ATGGAGATTG GACTTGAGAG GGGGTTCCAC AATGCATTGA TTGAGTTTGT GCTGATGCAG 
TTACAACTTG CTTCCGTCTT TTTCACGTTT CAGCTCGGGA CAAAAACACA TTACTACGGA 
AGGACATTGT TTCATGGAGG CGCAGAGTAT AGAGGAACTG GTCGAGGGTT TGTGGTCTTC 
CACGCGAAAT TTGCTGAGAA CTATAGGTTC TATTCCCGTA GTCACTTTGT GAAGGGCATT 
GAACTTATGA TTCTTCTACT GGTATATCAG ATCTTTGGTC AATCATATAG GGGTGTGGTT 
ACTTATATTC TGATCACTGT TTCGATATGG TTCATGGTGG TGACATGGCT CTTTGCTCCT 
TTCCTTTTCA ATCCGTCTGG TTTTGAGTGG CAAAAGATTG TTGATGACTG GACAGACTGG 
AACAAATGGA TTTATAACCG AGGAGGAATT GGTGTCCCTC CTGAGAAGAG TTGGGAATCT 
TGGTGGGAGA AAGAGTTAGA ACATCTTAGA CATTCAGGGG TTCGTGGTAT CACCCTTGAG 
ATCTTTTTGG CCTTGCGCTT CTTCATCTTT CAATATGGAC TTGTGTATCA TCTCAGTACC 
TTCAAAGGAA AAAATCAGAG CTTTTGGGTT TATGGAGCGT CATGGTTTGT GATCTTATTC 
ATTCTACTGA TTGTGAAGGG TTTGGGCGTT GGAAGGCGAA GATTCAGCAC GAATTTCCAG 
CTTCTATTCA GAATCATAAA GGGTCTTGTG TTCCTCACAT TTGTGGCAAT TCTCATAACT 
TTCCTCGCAC TTCCTCTGAT AACAATCAAA GACTTATTCA TCTGCATGCT TGCCTTCATG 
CCAACTGGAT GGGGAATGCT TCTCATTGCA CAAGCGTGTA AGCCTCTGAT CCAACAGCTA 
GGGATATGGT CATCAGTTAG GACGCTAGCT CGAGGCTACG AGATCGTGAT GGGCTTGCTT 
CTGTTCACAC CAGTTGCGTT TTTGGCCTGG TTTCCTTTTG TGTCTGAGTT CCAGACAAGA 
ATGCTCTTTA ACCAAGCCTT TAGTAGAGGT CTACAAATCT CTCGTATTCT TGGTGGTCAA 
AGAAAAGACC GGTCTTCAAA AAACAAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_563743.2
CDS	379..6231
Translation	MAQRREPDPPPPQRRILRTQTVGSLGEAMLDSEVVPSSLVEIAPILRVANEVEASNPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALLQRLERENETTLAGRQKSDAREMQSFYQHYYKKYIQALLNAADKADRAQLTKAYQTAAVLFEVLKAVNQTEDVEVADEILETHNKVEEKTQIYVPYNILPLDPDSQNQAIMRLPEIQAAVAALRNTRGLPWTAGHKKKLDEDILDWLQSMFGFQKDNVLNQREHLILLLANVHIRQFPKPDQQPKLDDRALTIVMKKLFRNYKKWCKYLGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGSVSPMTGEHVKPAYGGEDEAFLQKVVTPIYQTISKEAKRSRGGKSKHSVWRNYDDLNEYFWSIRCFRLGWPMRADADFFCQTAEELRLERSEIKSNSGDRWMGKVNFVEIRSFWHIFRSFDRLWSFYILCLQAMIVIAWNGSGELSAIFQGDVFLKVLSVFITAAILKLAQAVLDIALSWKARHSMSLYVKLRYVMKVGAAAVWVVVMAVTYAYSWKNASGFSQTIKNWFGGHSHNSPSLFIVAILIYLSPNMLSALLFLFPFIRRYLERSDYKIMMLMMWWSQPRLYIGRGMHESALSLFKYTMFWIVLLISKLAFSYYAEIKPLVGPTKDIMRIHISVYSWHEFFPHAKNNLGVVIALWSPVILVYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSIPGAFNDCLVPQDNSDDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISSFREEDLISDREMELLLVPYWSDPDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELKKRLAVDSYMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALPDLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLERVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEELLKGYKALELTSEEASKSGGSLWAQCQALADMKFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYKGTEEKIYYSALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE

Target ORF information:

RefSeq Version	NM_100436.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_100436.5

ORF Insert Sequence:

ATGGCTCAAA GAAGGGAACC TGATCCGCCT CCACCGCAGA GGCGGATTCT GCGGACTCAG 
ACTGTTGGTA GTCTCGGAGA GGCTATGCTT GATAGTGAAG TTGTTCCCTC TTCTTTAGTT 
GAGATTGCTC CGATTCTACG TGTGGCTAAT GAAGTTGAAG CTAGTAATCC ACGAGTTGCT 
TACCTATGTC GTTTCTACGC ATTTGAGAAG GCGCATAGGC TTGATCCAAC TTCAAGTGGT 
CGTGGTGTTC GCCAGTTTAA GACCGCGCTT CTTCAACGAT TAGAAAGGGA GAATGAAACA 
ACTTTGGCAG GAAGGCAAAA GAGTGATGCA CGTGAAATGC AGAGTTTCTA TCAACACTAC 
TACAAGAAAT ACATCCAAGC TTTGCTGAAT GCTGCGGATA AAGCTGACCG TGCTCAACTT 
ACAAAGGCCT ATCAAACTGC TGCCGTTCTC TTTGAAGTCT TGAAGGCTGT TAATCAAACA 
GAAGATGTGG AAGTGGCTGA TGAGATTTTG GAAACGCACA ATAAGGTGGA AGAGAAGACC 
CAGATTTACG TGCCTTACAA TATACTTCCC CTTGACCCTG ATAGTCAGAA TCAGGCAATA 
ATGAGATTGC CTGAGATACA AGCTGCTGTA GCCGCTCTTA GAAATACAAG GGGATTACCA 
TGGACAGCAG GTCACAAGAA GAAACTCGAT GAAGACATTC TAGACTGGCT TCAGTCTATG 
TTTGGTTTTC AGAAAGATAA TGTTTTGAAT CAAAGAGAGC ACTTGATCTT GTTACTTGCT 
AATGTCCACA TCCGACAATT TCCAAAACCT GACCAACAAC CAAAGTTGGA TGATCGTGCC 
TTGACCATAG TGATGAAGAA ACTTTTCAGA AACTACAAAA AGTGGTGCAA ATACTTGGGT 
CGGAAAAGCA GCCTTTGGTT GCCTACTATT CAGCAAGAGG TACAGCAGCG TAAACTGCTC 
TACATGGGAC TTTATCTATT AATATGGGGA GAAGCTGCAA ACCTGAGATT CATGCCAGAA 
TGCCTCTGTT ATATATATCA CCATATGGCT TTCGAGCTTT ATGGTATGTT GGCCGGTAGT 
GTTAGTCCCA TGACAGGGGA GCATGTAAAG CCAGCGTATG GTGGTGAAGA TGAAGCTTTC 
CTGCAGAAAG TTGTGACTCC TATATACCAG ACAATATCAA AGGAAGCAAA GAGAAGTAGA 
GGAGGAAAGT CAAAGCATTC TGTGTGGAGA AACTATGATG ACTTGAATGA ATATTTTTGG 
TCAATTCGAT GTTTTCGCTT AGGGTGGCCA ATGCGGGCTG ATGCTGATTT CTTTTGTCAG 
ACAGCTGAAG AACTTCGACT TGAGAGAAGT GAGATAAAAT CAAACAGTGG AGATCGGTGG 
ATGGGCAAAG TCAATTTTGT GGAGATACGC TCGTTCTGGC ATATATTTAG AAGTTTTGAT 
AGATTGTGGA GCTTTTATAT CCTGTGTTTG CAGGCAATGA TTGTTATTGC ATGGAATGGA 
TCCGGAGAGT TAAGCGCCAT TTTTCAGGGT GATGTCTTCC TAAAGGTTTT GAGCGTCTTC 
ATTACAGCAG CTATACTAAA GCTTGCACAA GCTGTCCTTG ACATAGCCCT CAGCTGGAAG 
GCAAGACATA GTATGTCACT CTATGTGAAA CTCAGATACG TCATGAAGGT AGGTGCGGCT 
GCAGTATGGG TTGTAGTCAT GGCAGTTACT TATGCGTACA GCTGGAAAAA CGCCTCCGGT 
TTTTCTCAGA CCATAAAGAA TTGGTTTGGG GGACACAGCC ATAATTCACC TTCTCTTTTC 
ATAGTGGCAA TTCTTATCTA CTTATCCCCG AACATGCTTT CTGCGTTGCT GTTTTTGTTT 
CCATTCATTC GGCGCTATCT TGAAAGGTCT GACTATAAGA TAATGATGCT GATGATGTGG 
TGGTCTCAGC CTCGTCTGTA TATAGGAAGG GGTATGCATG AGAGCGCTTT GTCACTTTTC 
AAGTACACAA TGTTTTGGAT CGTTCTGCTG ATTTCAAAGC TGGCATTCAG TTATTACGCT 
GAGATTAAGC CCCTTGTTGG TCCAACCAAA GATATAATGC GAATCCATAT ATCCGTCTAC 
AGCTGGCATG AGTTTTTCCC TCACGCCAAG AATAATTTAG GAGTCGTCAT CGCGCTCTGG 
TCACCAGTTA TTCTCGTTTA TTTCATGGAC ACTCAAATAT GGTATGCTAT AGTTTCAACT 
CTGGTCGGAG GTTTAAATGG AGCTTTCCGC CGTCTTGGAG AGATCCGAAC ACTTGGAATG 
TTGAGGTCGA GATTTCAGTC CATACCAGGG GCCTTTAATG ATTGCTTGGT ACCACAAGAT 
AACAGCGATG ATACAAAGAA AAAGAGATTT AGGGCGACCT TCTCTCGCAA GTTTGATCAG 
CTCCCATCTA GTAAAGACAA GGAAGCAGCG CGGTTTGCTC AGATGTGGAA CAAAATAATC 
AGTAGTTTCC GAGAAGAGGA CTTGATTAGT GATAGGGAAA TGGAACTATT GCTTGTGCCA 
TATTGGTCTG ACCCTGATTT GGATCTTATC CGGTGGCCAC CTTTTCTATT GGCTAGTAAG 
ATCCCAATAG CTTTGGATAT GGCTAAAGAC AGCAATGGAA AAGACCGTGA ACTGAAAAAG 
AGATTAGCTG TTGACAGCTA CATGACTTGT GCAGTTAGAG AGTGCTATGC CTCTTTTAAG 
AATCTCATCA ATTATTTGGT GGTCGGAGAA CGAGAAGGAC AGGTCATAAA CGACATTTTC 
TCCAAGATTG ATGAACATAT TGAGAAGGAA ACGCTGATAA CAGAATTGAA CCTGAGTGCT 
CTTCCTGATC TGTATGGCCA GTTCGTTCGG CTAATCGAAT ATTTGCTAGA AAACAGGGAG 
GAGGACAAGG ATCAAATTGT AATTGTCTTG CTGAACATGT TAGAATTGGT GACTAGAGAC 
ATCATGGAAG AAGAAGTCCC AAGCTTACTA GAGACTGCCC ACAATGGATC TTATGTCAAG 
TATGACGTCA TGACCCCTCT TCATCAACAG CGCAAATATT TCAGTCAACT TCGATTCCCA 
GTTTATTCTC AAACGGAAGC TTGGAAAGAA AAAATTAAGA GGCTTCATCT TTTGCTTACT 
GTCAAGGAGT CAGCTATGGA TGTTCCATCC AACTTGGAAG CTCGAAGGCG TCTTACTTTC 
TTTTCAAATT CACTATTCAT GGACATGCCT CCTGCACCCA AGATCCGCAA CATGCTTTCC 
TTCTCGGTCC TTACACCATA CTTTTCAGAG GATGTTCTTT TCTCCATATT TGGCTTAGAA 
CAGCAAAATG AAGATGGAGT TTCTATACTC TTTTACTTGC AGAAGATCTT CCCAGATGAA 
TGGACAAACT TTCTGGAACG AGTTAAATGT GGCAACGAAG AAGAACTAAG AGCAAGAGAG 
GATTTGGAAG AGGAGCTTCG TCTTTGGGCA TCTTACAGAG GCCAAACTCT GACTAAAACT 
GTGCGAGGAA TGATGTATTA CCGAAAGGCT TTGGAACTTC AGGCCTTCCT TGATATGGCA 
AAGGATGAAG AATTATTAAA AGGCTACAAG GCTCTCGAGT TAACCAGTGA AGAAGCATCA 
AAGAGCGGAG GGTCACTTTG GGCGCAGTGT CAAGCACTTG CAGATATGAA GTTCACCTTT 
GTGGTTTCTT GTCAACAATA CAGTATTCAT AAGAGATCTG GCGACCAACG TGCTAAAGAC 
ATATTGAGAC TTATGACGAC GTATCCGTCT ATTCGTGTCG CCTATATCGA TGAGGTAGAG 
CAAACACATA AAGAAAGTTA TAAGGGGACA GAGGAGAAAA TTTATTACTC AGCGCTTGTA 
AAAGCTGCTC CACAGACCAA ACCAATGGAT TCTTCTGAAT CTGTTCAAAC ACTAGATCAG 
CTCATTTATC GGATTAAGCT TCCGGGACCA GCTATACTAG GAGAGGGAAA GCCGGAAAAT 
CAGAACCATG CTATCATATT TACCCGTGGA GAAGGGTTGC AGACGATAGA CATGAATCAG 
GACAACTACA TGGAGGAAGC TTTCAAAATG AGGAACTTGC TGCAAGAGTT TCTTGAAAAG 
CATGGAGGCG TAAGATGTCC TACGATTCTT GGTCTTAGAG AGCATATTTT CACTGGAAGT 
GTGTCTTCTC TTGCATGGTT TATGTCAAAT CAAGAGAACA GTTTTGTAAC GATTGGGCAA 
AGAGTGCTAG CTAGTCCCTT GAAAGTACGA TTCCATTACG GACATCCAGA TATTTTTGAT 
CGTCTGTTTC ACCTTACCAG AGGTGGTATC TGCAAAGCTT CTAAAGTCAT CAATCTTAGT 
GAAGACATAT TTGCAGGTTT TAATTCTACT CTGCGTGAAG GAAATGTGAC TCATCATGAA 
TATATACAAG TTGGTAAAGG GAGAGATGTG GGCCTCAACC AGATCTCAAT GTTTGAGGCA 
AAAATCGCCA ATGGAAATGG CGAGCAAACT TTGAGTCGCG ACCTTTATAG GCTAGGACAC 
CGATTTGATT TCTTCCGGAT GCTGTCTTGT TATTTCACCA CAATTGGGTT CTACTTCAGT 
ACCATGTTAA CCGTGCTTAC GGTGTATGTG TTTCTTTATG GACGGTTATA TCTAGTACTT 
AGTGGGCTTG AAGAAGGATT GAGTAGCCAA CGAGCTTTCC GTAACAATAA GCCTCTTGAG 
GCAGCTCTTG CTTCTCAATC ATTTGTGCAG ATTGGTTTTT TAATGGCTTT GCCTATGATG 
ATGGAGATTG GACTTGAGAG GGGGTTCCAC AATGCATTGA TTGAGTTTGT GCTGATGCAG 
TTACAACTTG CTTCCGTCTT TTTCACGTTT CAGCTCGGGA CAAAAACACA TTACTACGGA 
AGGACATTGT TTCATGGAGG CGCAGAGTAT AGAGGAACTG GTCGAGGGTT TGTGGTCTTC 
CACGCGAAAT TTGCTGAGAA CTATAGGTTC TATTCCCGTA GTCACTTTGT GAAGGGCATT 
GAACTTATGA TTCTTCTACT GGTATATCAG ATCTTTGGTC AATCATATAG GGGTGTGGTT 
ACTTATATTC TGATCACTGT TTCGATATGG TTCATGGTGG TGACATGGCT CTTTGCTCCT 
TTCCTTTTCA ATCCGTCTGG TTTTGAGTGG CAAAAGATTG TTGATGACTG GACAGACTGG 
AACAAATGGA TTTATAACCG AGGAGGAATT GGTGTCCCTC CTGAGAAGAG TTGGGAATCT 
TGGTGGGAGA AAGAGTTAGA ACATCTTAGA CATTCAGGGG TTCGTGGTAT CACCCTTGAG 
ATCTTTTTGG CCTTGCGCTT CTTCATCTTT CAATATGGAC TTGTGTATCA TCTCAGTACC 
TTCAAAGGAA AAAATCAGAG CTTTTGGGTT TATGGAGCGT CATGGTTTGT GATCTTATTC 
ATTCTACTGA TTGTGAAGGG TTTGGGCGTT GGAAGGCGAA GATTCAGCAC GAATTTCCAG 
CTTCTATTCA GAATCATAAA GGGTCTTGTG TTCCTCACAT TTGTGGCAAT TCTCATAACT 
TTCCTCGCAC TTCCTCTGAT AACAATCAAA GACTTATTCA TCTGCATGCT TGCCTTCATG 
CCAACTGGAT GGGGAATGCT TCTCATTGCA CAAGCGTGTA AGCCTCTGAT CCAACAGCTA 
GGGATATGGT CATCAGTTAG GACGCTAGCT CGAGGCTACG AGATCGTGAT GGGCTTGCTT 
CTGTTCACAC CAGTTGCGTT TTTGGCCTGG TTTCCTTTTG TGTCTGAGTT CCAGACAAGA 
ATGCTCTTTA ACCAAGCCTT TAGTAGAGGT CTACAAATCT CTCGTATTCT TGGTGGTCAA 
AGAAAAGACC GGTCTTCAAA AAACAAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CALS1 ( NM_001331556.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322483.1 Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.

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CloneID	OAb22755
Clone ID Related Accession (Same CDS sequence)	NM_001331555.1 , NM_100436.5 , NM_001331556.1
Accession Version	NM_001331556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	callose synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCTCAAA GAAGGGAACC TGATCCGCCT CCACCGCAGA GGCGGATTCT GCGGACTCAG 
ACTGTTGGTA GTCTCGGAGA GGCTATGCTT GATAGTGAAG TTGTTCCCTC TTCTTTAGTT 
GAGATTGCTC CGATTCTACG TGTGGCTAAT GAAGTTGAAG CTAGTAATCC ACGAGTTGCT 
TACCTATGTC GTTTCTACGC ATTTGAGAAG GCGCATAGGC TTGATCCAAC TTCAAGTGGT 
CGTGGTGTTC GCCAGTTTAA GACCGCGCTT CTTCAACGAT TAGAAAGGGA GAATGAAACA 
ACTTTGGCAG GAAGGCAAAA GAGTGATGCA CGTGAAATGC AGAGTTTCTA TCAACACTAC 
TACAAGAAAT ACATCCAAGC TTTGCTGAAT GCTGCGGATA AAGCTGACCG TGCTCAACTT 
ACAAAGGCCT ATCAAACTGC TGCCGTTCTC TTTGAAGTCT TGAAGGCTGT TAATCAAACA 
GAAGATGTGG AAGTGGCTGA TGAGATTTTG GAAACGCACA ATAAGGTGGA AGAGAAGACC 
CAGATTTACG TGCCTTACAA TATACTTCCC CTTGACCCTG ATAGTCAGAA TCAGGCAATA 
ATGAGATTGC CTGAGATACA AGCTGCTGTA GCCGCTCTTA GAAATACAAG GGGATTACCA 
TGGACAGCAG GTCACAAGAA GAAACTCGAT GAAGACATTC TAGACTGGCT TCAGTCTATG 
TTTGGTTTTC AGAAAGATAA TGTTTTGAAT CAAAGAGAGC ACTTGATCTT GTTACTTGCT 
AATGTCCACA TCCGACAATT TCCAAAACCT GACCAACAAC CAAAGTTGGA TGATCGTGCC 
TTGACCATAG TGATGAAGAA ACTTTTCAGA AACTACAAAA AGTGGTGCAA ATACTTGGGT 
CGGAAAAGCA GCCTTTGGTT GCCTACTATT CAGCAAGAGG TACAGCAGCG TAAACTGCTC 
TACATGGGAC TTTATCTATT AATATGGGGA GAAGCTGCAA ACCTGAGATT CATGCCAGAA 
TGCCTCTGTT ATATATATCA CCATATGGCT TTCGAGCTTT ATGGTATGTT GGCCGGTAGT 
GTTAGTCCCA TGACAGGGGA GCATGTAAAG CCAGCGTATG GTGGTGAAGA TGAAGCTTTC 
CTGCAGAAAG TTGTGACTCC TATATACCAG ACAATATCAA AGGAAGCAAA GAGAAGTAGA 
GGAGGAAAGT CAAAGCATTC TGTGTGGAGA AACTATGATG ACTTGAATGA ATATTTTTGG 
TCAATTCGAT GTTTTCGCTT AGGGTGGCCA ATGCGGGCTG ATGCTGATTT CTTTTGTCAG 
ACAGCTGAAG AACTTCGACT TGAGAGAAGT GAGATAAAAT CAAACAGTGG AGATCGGTGG 
ATGGGCAAAG TCAATTTTGT GGAGATACGC TCGTTCTGGC ATATATTTAG AAGTTTTGAT 
AGATTGTGGA GCTTTTATAT CCTGTGTTTG CAGGCAATGA TTGTTATTGC ATGGAATGGA 
TCCGGAGAGT TAAGCGCCAT TTTTCAGGGT GATGTCTTCC TAAAGGTTTT GAGCGTCTTC 
ATTACAGCAG CTATACTAAA GCTTGCACAA GCTGTCCTTG ACATAGCCCT CAGCTGGAAG 
GCAAGACATA GTATGTCACT CTATGTGAAA CTCAGATACG TCATGAAGGT AGGTGCGGCT 
GCAGTATGGG TTGTAGTCAT GGCAGTTACT TATGCGTACA GCTGGAAAAA CGCCTCCGGT 
TTTTCTCAGA CCATAAAGAA TTGGTTTGGG GGACACAGCC ATAATTCACC TTCTCTTTTC 
ATAGTGGCAA TTCTTATCTA CTTATCCCCG AACATGCTTT CTGCGTTGCT GTTTTTGTTT 
CCATTCATTC GGCGCTATCT TGAAAGGTCT GACTATAAGA TAATGATGCT GATGATGTGG 
TGGTCTCAGC CTCGTCTGTA TATAGGAAGG GGTATGCATG AGAGCGCTTT GTCACTTTTC 
AAGTACACAA TGTTTTGGAT CGTTCTGCTG ATTTCAAAGC TGGCATTCAG TTATTACGCT 
GAGATTAAGC CCCTTGTTGG TCCAACCAAA GATATAATGC GAATCCATAT ATCCGTCTAC 
AGCTGGCATG AGTTTTTCCC TCACGCCAAG AATAATTTAG GAGTCGTCAT CGCGCTCTGG 
TCACCAGTTA TTCTCGTTTA TTTCATGGAC ACTCAAATAT GGTATGCTAT AGTTTCAACT 
CTGGTCGGAG GTTTAAATGG AGCTTTCCGC CGTCTTGGAG AGATCCGAAC ACTTGGAATG 
TTGAGGTCGA GATTTCAGTC CATACCAGGG GCCTTTAATG ATTGCTTGGT ACCACAAGAT 
AACAGCGATG ATACAAAGAA AAAGAGATTT AGGGCGACCT TCTCTCGCAA GTTTGATCAG 
CTCCCATCTA GTAAAGACAA GGAAGCAGCG CGGTTTGCTC AGATGTGGAA CAAAATAATC 
AGTAGTTTCC GAGAAGAGGA CTTGATTAGT GATAGGGAAA TGGAACTATT GCTTGTGCCA 
TATTGGTCTG ACCCTGATTT GGATCTTATC CGGTGGCCAC CTTTTCTATT GGCTAGTAAG 
ATCCCAATAG CTTTGGATAT GGCTAAAGAC AGCAATGGAA AAGACCGTGA ACTGAAAAAG 
AGATTAGCTG TTGACAGCTA CATGACTTGT GCAGTTAGAG AGTGCTATGC CTCTTTTAAG 
AATCTCATCA ATTATTTGGT GGTCGGAGAA CGAGAAGGAC AGGTCATAAA CGACATTTTC 
TCCAAGATTG ATGAACATAT TGAGAAGGAA ACGCTGATAA CAGAATTGAA CCTGAGTGCT 
CTTCCTGATC TGTATGGCCA GTTCGTTCGG CTAATCGAAT ATTTGCTAGA AAACAGGGAG 
GAGGACAAGG ATCAAATTGT AATTGTCTTG CTGAACATGT TAGAATTGGT GACTAGAGAC 
ATCATGGAAG AAGAAGTCCC AAGCTTACTA GAGACTGCCC ACAATGGATC TTATGTCAAG 
TATGACGTCA TGACCCCTCT TCATCAACAG CGCAAATATT TCAGTCAACT TCGATTCCCA 
GTTTATTCTC AAACGGAAGC TTGGAAAGAA AAAATTAAGA GGCTTCATCT TTTGCTTACT 
GTCAAGGAGT CAGCTATGGA TGTTCCATCC AACTTGGAAG CTCGAAGGCG TCTTACTTTC 
TTTTCAAATT CACTATTCAT GGACATGCCT CCTGCACCCA AGATCCGCAA CATGCTTTCC 
TTCTCGGTCC TTACACCATA CTTTTCAGAG GATGTTCTTT TCTCCATATT TGGCTTAGAA 
CAGCAAAATG AAGATGGAGT TTCTATACTC TTTTACTTGC AGAAGATCTT CCCAGATGAA 
TGGACAAACT TTCTGGAACG AGTTAAATGT GGCAACGAAG AAGAACTAAG AGCAAGAGAG 
GATTTGGAAG AGGAGCTTCG TCTTTGGGCA TCTTACAGAG GCCAAACTCT GACTAAAACT 
GTGCGAGGAA TGATGTATTA CCGAAAGGCT TTGGAACTTC AGGCCTTCCT TGATATGGCA 
AAGGATGAAG AATTATTAAA AGGCTACAAG GCTCTCGAGT TAACCAGTGA AGAAGCATCA 
AAGAGCGGAG GGTCACTTTG GGCGCAGTGT CAAGCACTTG CAGATATGAA GTTCACCTTT 
GTGGTTTCTT GTCAACAATA CAGTATTCAT AAGAGATCTG GCGACCAACG TGCTAAAGAC 
ATATTGAGAC TTATGACGAC GTATCCGTCT ATTCGTGTCG CCTATATCGA TGAGGTAGAG 
CAAACACATA AAGAAAGTTA TAAGGGGACA GAGGAGAAAA TTTATTACTC AGCGCTTGTA 
AAAGCTGCTC CACAGACCAA ACCAATGGAT TCTTCTGAAT CTGTTCAAAC ACTAGATCAG 
CTCATTTATC GGATTAAGCT TCCGGGACCA GCTATACTAG GAGAGGGAAA GCCGGAAAAT 
CAGAACCATG CTATCATATT TACCCGTGGA GAAGGGTTGC AGACGATAGA CATGAATCAG 
GACAACTACA TGGAGGAAGC TTTCAAAATG AGGAACTTGC TGCAAGAGTT TCTTGAAAAG 
CATGGAGGCG TAAGATGTCC TACGATTCTT GGTCTTAGAG AGCATATTTT CACTGGAAGT 
GTGTCTTCTC TTGCATGGTT TATGTCAAAT CAAGAGAACA GTTTTGTAAC GATTGGGCAA 
AGAGTGCTAG CTAGTCCCTT GAAAGTACGA TTCCATTACG GACATCCAGA TATTTTTGAT 
CGTCTGTTTC ACCTTACCAG AGGTGGTATC TGCAAAGCTT CTAAAGTCAT CAATCTTAGT 
GAAGACATAT TTGCAGGTTT TAATTCTACT CTGCGTGAAG GAAATGTGAC TCATCATGAA 
TATATACAAG TTGGTAAAGG GAGAGATGTG GGCCTCAACC AGATCTCAAT GTTTGAGGCA 
AAAATCGCCA ATGGAAATGG CGAGCAAACT TTGAGTCGCG ACCTTTATAG GCTAGGACAC 
CGATTTGATT TCTTCCGGAT GCTGTCTTGT TATTTCACCA CAATTGGGTT CTACTTCAGT 
ACCATGTTAA CCGTGCTTAC GGTGTATGTG TTTCTTTATG GACGGTTATA TCTAGTACTT 
AGTGGGCTTG AAGAAGGATT GAGTAGCCAA CGAGCTTTCC GTAACAATAA GCCTCTTGAG 
GCAGCTCTTG CTTCTCAATC ATTTGTGCAG ATTGGTTTTT TAATGGCTTT GCCTATGATG 
ATGGAGATTG GACTTGAGAG GGGGTTCCAC AATGCATTGA TTGAGTTTGT GCTGATGCAG 
TTACAACTTG CTTCCGTCTT TTTCACGTTT CAGCTCGGGA CAAAAACACA TTACTACGGA 
AGGACATTGT TTCATGGAGG CGCAGAGTAT AGAGGAACTG GTCGAGGGTT TGTGGTCTTC 
CACGCGAAAT TTGCTGAGAA CTATAGGTTC TATTCCCGTA GTCACTTTGT GAAGGGCATT 
GAACTTATGA TTCTTCTACT GGTATATCAG ATCTTTGGTC AATCATATAG GGGTGTGGTT 
ACTTATATTC TGATCACTGT TTCGATATGG TTCATGGTGG TGACATGGCT CTTTGCTCCT 
TTCCTTTTCA ATCCGTCTGG TTTTGAGTGG CAAAAGATTG TTGATGACTG GACAGACTGG 
AACAAATGGA TTTATAACCG AGGAGGAATT GGTGTCCCTC CTGAGAAGAG TTGGGAATCT 
TGGTGGGAGA AAGAGTTAGA ACATCTTAGA CATTCAGGGG TTCGTGGTAT CACCCTTGAG 
ATCTTTTTGG CCTTGCGCTT CTTCATCTTT CAATATGGAC TTGTGTATCA TCTCAGTACC 
TTCAAAGGAA AAAATCAGAG CTTTTGGGTT TATGGAGCGT CATGGTTTGT GATCTTATTC 
ATTCTACTGA TTGTGAAGGG TTTGGGCGTT GGAAGGCGAA GATTCAGCAC GAATTTCCAG 
CTTCTATTCA GAATCATAAA GGGTCTTGTG TTCCTCACAT TTGTGGCAAT TCTCATAACT 
TTCCTCGCAC TTCCTCTGAT AACAATCAAA GACTTATTCA TCTGCATGCT TGCCTTCATG 
CCAACTGGAT GGGGAATGCT TCTCATTGCA CAAGCGTGTA AGCCTCTGAT CCAACAGCTA 
GGGATATGGT CATCAGTTAG GACGCTAGCT CGAGGCTACG AGATCGTGAT GGGCTTGCTT 
CTGTTCACAC CAGTTGCGTT TTTGGCCTGG TTTCCTTTTG TGTCTGAGTT CCAGACAAGA 
ATGCTCTTTA ACCAAGCCTT TAGTAGAGGT CTACAAATCT CTCGTATTCT TGGTGGTCAA 
AGAAAAGACC GGTCTTCAAA AAACAAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322483.1
CDS	130..5982
Translation	MAQRREPDPPPPQRRILRTQTVGSLGEAMLDSEVVPSSLVEIAPILRVANEVEASNPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALLQRLERENETTLAGRQKSDAREMQSFYQHYYKKYIQALLNAADKADRAQLTKAYQTAAVLFEVLKAVNQTEDVEVADEILETHNKVEEKTQIYVPYNILPLDPDSQNQAIMRLPEIQAAVAALRNTRGLPWTAGHKKKLDEDILDWLQSMFGFQKDNVLNQREHLILLLANVHIRQFPKPDQQPKLDDRALTIVMKKLFRNYKKWCKYLGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGSVSPMTGEHVKPAYGGEDEAFLQKVVTPIYQTISKEAKRSRGGKSKHSVWRNYDDLNEYFWSIRCFRLGWPMRADADFFCQTAEELRLERSEIKSNSGDRWMGKVNFVEIRSFWHIFRSFDRLWSFYILCLQAMIVIAWNGSGELSAIFQGDVFLKVLSVFITAAILKLAQAVLDIALSWKARHSMSLYVKLRYVMKVGAAAVWVVVMAVTYAYSWKNASGFSQTIKNWFGGHSHNSPSLFIVAILIYLSPNMLSALLFLFPFIRRYLERSDYKIMMLMMWWSQPRLYIGRGMHESALSLFKYTMFWIVLLISKLAFSYYAEIKPLVGPTKDIMRIHISVYSWHEFFPHAKNNLGVVIALWSPVILVYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSIPGAFNDCLVPQDNSDDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISSFREEDLISDREMELLLVPYWSDPDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELKKRLAVDSYMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALPDLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLERVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEELLKGYKALELTSEEASKSGGSLWAQCQALADMKFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYKGTEEKIYYSALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE

Target ORF information:

RefSeq Version	NM_001331556.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331556.1

ORF Insert Sequence:

ATGGCTCAAA GAAGGGAACC TGATCCGCCT CCACCGCAGA GGCGGATTCT GCGGACTCAG 
ACTGTTGGTA GTCTCGGAGA GGCTATGCTT GATAGTGAAG TTGTTCCCTC TTCTTTAGTT 
GAGATTGCTC CGATTCTACG TGTGGCTAAT GAAGTTGAAG CTAGTAATCC ACGAGTTGCT 
TACCTATGTC GTTTCTACGC ATTTGAGAAG GCGCATAGGC TTGATCCAAC TTCAAGTGGT 
CGTGGTGTTC GCCAGTTTAA GACCGCGCTT CTTCAACGAT TAGAAAGGGA GAATGAAACA 
ACTTTGGCAG GAAGGCAAAA GAGTGATGCA CGTGAAATGC AGAGTTTCTA TCAACACTAC 
TACAAGAAAT ACATCCAAGC TTTGCTGAAT GCTGCGGATA AAGCTGACCG TGCTCAACTT 
ACAAAGGCCT ATCAAACTGC TGCCGTTCTC TTTGAAGTCT TGAAGGCTGT TAATCAAACA 
GAAGATGTGG AAGTGGCTGA TGAGATTTTG GAAACGCACA ATAAGGTGGA AGAGAAGACC 
CAGATTTACG TGCCTTACAA TATACTTCCC CTTGACCCTG ATAGTCAGAA TCAGGCAATA 
ATGAGATTGC CTGAGATACA AGCTGCTGTA GCCGCTCTTA GAAATACAAG GGGATTACCA 
TGGACAGCAG GTCACAAGAA GAAACTCGAT GAAGACATTC TAGACTGGCT TCAGTCTATG 
TTTGGTTTTC AGAAAGATAA TGTTTTGAAT CAAAGAGAGC ACTTGATCTT GTTACTTGCT 
AATGTCCACA TCCGACAATT TCCAAAACCT GACCAACAAC CAAAGTTGGA TGATCGTGCC 
TTGACCATAG TGATGAAGAA ACTTTTCAGA AACTACAAAA AGTGGTGCAA ATACTTGGGT 
CGGAAAAGCA GCCTTTGGTT GCCTACTATT CAGCAAGAGG TACAGCAGCG TAAACTGCTC 
TACATGGGAC TTTATCTATT AATATGGGGA GAAGCTGCAA ACCTGAGATT CATGCCAGAA 
TGCCTCTGTT ATATATATCA CCATATGGCT TTCGAGCTTT ATGGTATGTT GGCCGGTAGT 
GTTAGTCCCA TGACAGGGGA GCATGTAAAG CCAGCGTATG GTGGTGAAGA TGAAGCTTTC 
CTGCAGAAAG TTGTGACTCC TATATACCAG ACAATATCAA AGGAAGCAAA GAGAAGTAGA 
GGAGGAAAGT CAAAGCATTC TGTGTGGAGA AACTATGATG ACTTGAATGA ATATTTTTGG 
TCAATTCGAT GTTTTCGCTT AGGGTGGCCA ATGCGGGCTG ATGCTGATTT CTTTTGTCAG 
ACAGCTGAAG AACTTCGACT TGAGAGAAGT GAGATAAAAT CAAACAGTGG AGATCGGTGG 
ATGGGCAAAG TCAATTTTGT GGAGATACGC TCGTTCTGGC ATATATTTAG AAGTTTTGAT 
AGATTGTGGA GCTTTTATAT CCTGTGTTTG CAGGCAATGA TTGTTATTGC ATGGAATGGA 
TCCGGAGAGT TAAGCGCCAT TTTTCAGGGT GATGTCTTCC TAAAGGTTTT GAGCGTCTTC 
ATTACAGCAG CTATACTAAA GCTTGCACAA GCTGTCCTTG ACATAGCCCT CAGCTGGAAG 
GCAAGACATA GTATGTCACT CTATGTGAAA CTCAGATACG TCATGAAGGT AGGTGCGGCT 
GCAGTATGGG TTGTAGTCAT GGCAGTTACT TATGCGTACA GCTGGAAAAA CGCCTCCGGT 
TTTTCTCAGA CCATAAAGAA TTGGTTTGGG GGACACAGCC ATAATTCACC TTCTCTTTTC 
ATAGTGGCAA TTCTTATCTA CTTATCCCCG AACATGCTTT CTGCGTTGCT GTTTTTGTTT 
CCATTCATTC GGCGCTATCT TGAAAGGTCT GACTATAAGA TAATGATGCT GATGATGTGG 
TGGTCTCAGC CTCGTCTGTA TATAGGAAGG GGTATGCATG AGAGCGCTTT GTCACTTTTC 
AAGTACACAA TGTTTTGGAT CGTTCTGCTG ATTTCAAAGC TGGCATTCAG TTATTACGCT 
GAGATTAAGC CCCTTGTTGG TCCAACCAAA GATATAATGC GAATCCATAT ATCCGTCTAC 
AGCTGGCATG AGTTTTTCCC TCACGCCAAG AATAATTTAG GAGTCGTCAT CGCGCTCTGG 
TCACCAGTTA TTCTCGTTTA TTTCATGGAC ACTCAAATAT GGTATGCTAT AGTTTCAACT 
CTGGTCGGAG GTTTAAATGG AGCTTTCCGC CGTCTTGGAG AGATCCGAAC ACTTGGAATG 
TTGAGGTCGA GATTTCAGTC CATACCAGGG GCCTTTAATG ATTGCTTGGT ACCACAAGAT 
AACAGCGATG ATACAAAGAA AAAGAGATTT AGGGCGACCT TCTCTCGCAA GTTTGATCAG 
CTCCCATCTA GTAAAGACAA GGAAGCAGCG CGGTTTGCTC AGATGTGGAA CAAAATAATC 
AGTAGTTTCC GAGAAGAGGA CTTGATTAGT GATAGGGAAA TGGAACTATT GCTTGTGCCA 
TATTGGTCTG ACCCTGATTT GGATCTTATC CGGTGGCCAC CTTTTCTATT GGCTAGTAAG 
ATCCCAATAG CTTTGGATAT GGCTAAAGAC AGCAATGGAA AAGACCGTGA ACTGAAAAAG 
AGATTAGCTG TTGACAGCTA CATGACTTGT GCAGTTAGAG AGTGCTATGC CTCTTTTAAG 
AATCTCATCA ATTATTTGGT GGTCGGAGAA CGAGAAGGAC AGGTCATAAA CGACATTTTC 
TCCAAGATTG ATGAACATAT TGAGAAGGAA ACGCTGATAA CAGAATTGAA CCTGAGTGCT 
CTTCCTGATC TGTATGGCCA GTTCGTTCGG CTAATCGAAT ATTTGCTAGA AAACAGGGAG 
GAGGACAAGG ATCAAATTGT AATTGTCTTG CTGAACATGT TAGAATTGGT GACTAGAGAC 
ATCATGGAAG AAGAAGTCCC AAGCTTACTA GAGACTGCCC ACAATGGATC TTATGTCAAG 
TATGACGTCA TGACCCCTCT TCATCAACAG CGCAAATATT TCAGTCAACT TCGATTCCCA 
GTTTATTCTC AAACGGAAGC TTGGAAAGAA AAAATTAAGA GGCTTCATCT TTTGCTTACT 
GTCAAGGAGT CAGCTATGGA TGTTCCATCC AACTTGGAAG CTCGAAGGCG TCTTACTTTC 
TTTTCAAATT CACTATTCAT GGACATGCCT CCTGCACCCA AGATCCGCAA CATGCTTTCC 
TTCTCGGTCC TTACACCATA CTTTTCAGAG GATGTTCTTT TCTCCATATT TGGCTTAGAA 
CAGCAAAATG AAGATGGAGT TTCTATACTC TTTTACTTGC AGAAGATCTT CCCAGATGAA 
TGGACAAACT TTCTGGAACG AGTTAAATGT GGCAACGAAG AAGAACTAAG AGCAAGAGAG 
GATTTGGAAG AGGAGCTTCG TCTTTGGGCA TCTTACAGAG GCCAAACTCT GACTAAAACT 
GTGCGAGGAA TGATGTATTA CCGAAAGGCT TTGGAACTTC AGGCCTTCCT TGATATGGCA 
AAGGATGAAG AATTATTAAA AGGCTACAAG GCTCTCGAGT TAACCAGTGA AGAAGCATCA 
AAGAGCGGAG GGTCACTTTG GGCGCAGTGT CAAGCACTTG CAGATATGAA GTTCACCTTT 
GTGGTTTCTT GTCAACAATA CAGTATTCAT AAGAGATCTG GCGACCAACG TGCTAAAGAC 
ATATTGAGAC TTATGACGAC GTATCCGTCT ATTCGTGTCG CCTATATCGA TGAGGTAGAG 
CAAACACATA AAGAAAGTTA TAAGGGGACA GAGGAGAAAA TTTATTACTC AGCGCTTGTA 
AAAGCTGCTC CACAGACCAA ACCAATGGAT TCTTCTGAAT CTGTTCAAAC ACTAGATCAG 
CTCATTTATC GGATTAAGCT TCCGGGACCA GCTATACTAG GAGAGGGAAA GCCGGAAAAT 
CAGAACCATG CTATCATATT TACCCGTGGA GAAGGGTTGC AGACGATAGA CATGAATCAG 
GACAACTACA TGGAGGAAGC TTTCAAAATG AGGAACTTGC TGCAAGAGTT TCTTGAAAAG 
CATGGAGGCG TAAGATGTCC TACGATTCTT GGTCTTAGAG AGCATATTTT CACTGGAAGT 
GTGTCTTCTC TTGCATGGTT TATGTCAAAT CAAGAGAACA GTTTTGTAAC GATTGGGCAA 
AGAGTGCTAG CTAGTCCCTT GAAAGTACGA TTCCATTACG GACATCCAGA TATTTTTGAT 
CGTCTGTTTC ACCTTACCAG AGGTGGTATC TGCAAAGCTT CTAAAGTCAT CAATCTTAGT 
GAAGACATAT TTGCAGGTTT TAATTCTACT CTGCGTGAAG GAAATGTGAC TCATCATGAA 
TATATACAAG TTGGTAAAGG GAGAGATGTG GGCCTCAACC AGATCTCAAT GTTTGAGGCA 
AAAATCGCCA ATGGAAATGG CGAGCAAACT TTGAGTCGCG ACCTTTATAG GCTAGGACAC 
CGATTTGATT TCTTCCGGAT GCTGTCTTGT TATTTCACCA CAATTGGGTT CTACTTCAGT 
ACCATGTTAA CCGTGCTTAC GGTGTATGTG TTTCTTTATG GACGGTTATA TCTAGTACTT 
AGTGGGCTTG AAGAAGGATT GAGTAGCCAA CGAGCTTTCC GTAACAATAA GCCTCTTGAG 
GCAGCTCTTG CTTCTCAATC ATTTGTGCAG ATTGGTTTTT TAATGGCTTT GCCTATGATG 
ATGGAGATTG GACTTGAGAG GGGGTTCCAC AATGCATTGA TTGAGTTTGT GCTGATGCAG 
TTACAACTTG CTTCCGTCTT TTTCACGTTT CAGCTCGGGA CAAAAACACA TTACTACGGA 
AGGACATTGT TTCATGGAGG CGCAGAGTAT AGAGGAACTG GTCGAGGGTT TGTGGTCTTC 
CACGCGAAAT TTGCTGAGAA CTATAGGTTC TATTCCCGTA GTCACTTTGT GAAGGGCATT 
GAACTTATGA TTCTTCTACT GGTATATCAG ATCTTTGGTC AATCATATAG GGGTGTGGTT 
ACTTATATTC TGATCACTGT TTCGATATGG TTCATGGTGG TGACATGGCT CTTTGCTCCT 
TTCCTTTTCA ATCCGTCTGG TTTTGAGTGG CAAAAGATTG TTGATGACTG GACAGACTGG 
AACAAATGGA TTTATAACCG AGGAGGAATT GGTGTCCCTC CTGAGAAGAG TTGGGAATCT 
TGGTGGGAGA AAGAGTTAGA ACATCTTAGA CATTCAGGGG TTCGTGGTAT CACCCTTGAG 
ATCTTTTTGG CCTTGCGCTT CTTCATCTTT CAATATGGAC TTGTGTATCA TCTCAGTACC 
TTCAAAGGAA AAAATCAGAG CTTTTGGGTT TATGGAGCGT CATGGTTTGT GATCTTATTC 
ATTCTACTGA TTGTGAAGGG TTTGGGCGTT GGAAGGCGAA GATTCAGCAC GAATTTCCAG 
CTTCTATTCA GAATCATAAA GGGTCTTGTG TTCCTCACAT TTGTGGCAAT TCTCATAACT 
TTCCTCGCAC TTCCTCTGAT AACAATCAAA GACTTATTCA TCTGCATGCT TGCCTTCATG 
CCAACTGGAT GGGGAATGCT TCTCATTGCA CAAGCGTGTA AGCCTCTGAT CCAACAGCTA 
GGGATATGGT CATCAGTTAG GACGCTAGCT CGAGGCTACG AGATCGTGAT GGGCTTGCTT 
CTGTTCACAC CAGTTGCGTT TTTGGCCTGG TTTCCTTTTG TGTCTGAGTT CCAGACAAGA 
ATGCTCTTTA ACCAAGCCTT TAGTAGAGGT CTACAAATCT CTCGTATTCT TGGTGGTCAA 
AGAAAAGACC GGTCTTCAAA AAACAAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CALS1 ( NM_001197984.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001184913.1 Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.

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CloneID	OAb29688
Clone ID Related Accession (Same CDS sequence)	NM_001197984.1
Accession Version	NM_001197984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	callose synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCTCAAA GAAGGGAACC TGATCCGCCT CCACCGCAGA GGCGGATTCT GCGGACTCAG 
ACTGTTGGTA GTCTCGGAGA GGCTATGCTT GATAGTGAAG TTGTTCCCTC TTCTTTAGTT 
GAGATTGCTC CGATTCTACG TGTGGCTAAT GAAGTTGAAG CTAGTAATCC ACGAGTTGCT 
TACCTATGTC GTTTCTACGC ATTTGAGAAG GCGCATAGGC TTGATCCAAC TTCAAGTGGT 
CGTGGTGTTC GCCAGTTTAA GACCGCGCTT CTTCAACGAT TAGAAAGGGA GAATGAAACA 
ACTTTGGCAG GAAGGCAAAA GAGTGATGCA CGTGAAATGC AGAGTTTCTA TCAACACTAC 
TACAAGAAAT ACATCCAAGC TTTGCTGAAT GCTGCGGATA AAGCTGACCG TGCTCAACTT 
ACAAAGGCCT ATCAAACTGC TGCCGTTCTC TTTGAAGTCT TGAAGGCTGT TAATCAAACA 
GAAGATGTGG AAGTGGCTGA TGAGATTTTG GAAACGCACA ATAAGGTGGA AGAGAAGACC 
CAGATTTACG TGCCTTACAA TATACTTCCC CTTGACCCTG ATAGTCAGAA TCAGGCAATA 
ATGAGATTGC CTGAGATACA AGCTGCTGTA GCCGCTCTTA GAAATACAAG GGGATTACCA 
TGGACAGCAG GTCACAAGAA GAAACTCGAT GAAGACATTC TAGACTGGCT TCAGTCTATG 
TTTGGTTTTC AGAAAGATAA TGTTTTGAAT CAAAGAGAGC ACTTGATCTT GTTACTTGCT 
AATGTCCACA TCCGACAATT TCCAAAACCT GACCAACAAC CAAAGTTGGA TGATCGTGCC 
TTGACCATAG TGATGAAGAA ACTTTTCAGA AACTACAAAA AGTGGTGCAA ATACTTGGGT 
CGGAAAAGCA GCCTTTGGTT GCCTACTATT CAGCAAGAGG TACAGCAGCG TAAACTGCTC 
TACATGGGAC TTTATCTATT AATATGGGGA GAAGCTGCAA ACCTGAGATT CATGCCAGAA 
TGCCTCTGTT ATATATATCA CCATATGGCT TTCGAGCTTT ATGGTATGTT GGCCGGTAGT 
GTTAGTCCCA TGACAGGGGA GCATGTAAAG CCAGCGTATG GTGGTGAAGA TGAAGCTTTC 
CTGCAGAAAG TTGTGACTCC TATATACCAG ACAATATCAA AGGAAGCAAA GAGAAGTAGA 
GGAGGAAAGT CAAAGCATTC TGTGTGGAGA AACTATGATG ACTTGAATGA ATATTTTTGG 
TCAATTCGAT GTTTTCGCTT AGGGTGGCCA ATGCGGGCTG ATGCTGATTT CTTTTGTCAG 
ACAGCTGAAG AACTTCGACT TGAGAGAAGT GAGATAAAAT CAAACAGTGG AGATCGGTGG 
ATGGGCAAAG TCAATTTTGT GGAGATACGC TCGTTCTGGC ATATATTTAG AAGTTTTGAT 
AGATTGTGGA GCTTTTATAT CCTGTGTTTG CAGGCAATGA TTGTTATTGC ATGGAATGGA 
TCCGGAGAGT TAAGCGCCAT TTTTCAGGGT GATGTCTTCC TAAAGGTTTT GAGCGTCTTC 
ATTACAGCAG CTATACTAAA GCTTGCACAA GCTGTCCTTG ACATAGCCCT CAGCTGGAAG 
GCAAGACATA GTATGTCACT CTATGTGAAA CTCAGATACG TCATGAAGGT AGGTGCGGCT 
GCAGTATGGG TTGTAGTCAT GGCAGTTACT TATGCGTACA GCTGGAAAAA CGCCTCCGGT 
TTTTCTCAGA CCATAAAGAA TTGGTCTGAC TATAAGATAA TGATGCTGAT GATGTGGTGG 
TCTCAGCCTC GTCTGTATAT AGGAAGGGGT ATGCATGAGA GCGCTTTGTC ACTTTTCAAG 
TACACAATGT TTTGGATCGT TCTGCTGATT TCAAAGCTGG CATTCAGTTA TTACGCTGAG 
ATTAAGCCCC TTGTTGGTCC AACCAAAGAT ATAATGCGAA TCCATATATC CGTCTACAGC 
TGGCATGAGT TTTTCCCTCA CGCCAAGAAT AATTTAGGAG TCGTCATCGC GCTCTGGTCA 
CCAGTTATTC TCGTTTATTT CATGGACACT CAAATATGGT ATGCTATAGT TTCAACTCTG 
GTCGGAGGTT TAAATGGAGC TTTCCGCCGT CTTGGAGAGA TCCGAACACT TGGAATGTTG 
AGGTCGAGAT TTCAGTCCAT ACCAGGGGCC TTTAATGATT GCTTGGTACC ACAAGATAAC 
AGCGATGATA CAAAGAAAAA GAGATTTAGG GCGACCTTCT CTCGCAAGTT TGATCAGCTC 
CCATCTAGTA AAGACAAGGA AGCAGCGCGG TTTGCTCAGA TGTGGAACAA AATAATCAGT 
AGTTTCCGAG AAGAGGACTT GATTAGTGAT AGGGAAATGG AACTATTGCT TGTGCCATAT 
TGGTCTGACC CTGATTTGGA TCTTATCCGG TGGCCACCTT TTCTATTGGC TAGTAAGATC 
CCAATAGCTT TGGATATGGC TAAAGACAGC AATGGAAAAG ACCGTGAACT GAAAAAGAGA 
TTAGCTGTTG ACAGCTACAT GACTTGTGCA GTTAGAGAGT GCTATGCCTC TTTTAAGAAT 
CTCATCAATT ATTTGGTGGT CGGAGAACGA GAAGGACAGG TCATAAACGA CATTTTCTCC 
AAGATTGATG AACATATTGA GAAGGAAACG CTGATAACAG AATTGAACCT GAGTGCTCTT 
CCTGATCTGT ATGGCCAGTT CGTTCGGCTA ATCGAATATT TGCTAGAAAA CAGGGAGGAG 
GACAAGGATC AAATTGTAAT TGTCTTGCTG AACATGTTAG AATTGGTGAC TAGAGACATC 
ATGGAAGAAG AAGTCCCAAG CTTACTAGAG ACTGCCCACA ATGGATCTTA TGTCAAGTAT 
GACGTCATGA CCCCTCTTCA TCAACAGCGC AAATATTTCA GTCAACTTCG ATTCCCAGTT 
TATTCTCAAA CGGAAGCTTG GAAAGAAAAA ATTAAGAGGC TTCATCTTTT GCTTACTGTC 
AAGGAGTCAG CTATGGATGT TCCATCCAAC TTGGAAGCTC GAAGGCGTCT TACTTTCTTT 
TCAAATTCAC TATTCATGGA CATGCCTCCT GCACCCAAGA TCCGCAACAT GCTTTCCTTC 
TCGGTCCTTA CACCATACTT TTCAGAGGAT GTTCTTTTCT CCATATTTGG CTTAGAACAG 
CAAAATGAAG ATGGAGTTTC TATACTCTTT TACTTGCAGA AGATCTTCCC AGATGAATGG 
ACAAACTTTC TGGAACGAGT TAAATGTGGC AACGAAGAAG AACTAAGAGC AAGAGAGGAT 
TTGGAAGAGG AGCTTCGTCT TTGGGCATCT TACAGAGGCC AAACTCTGAC TAAAACTGTG 
CGAGGAATGA TGTATTACCG AAAGGCTTTG GAACTTCAGG CCTTCCTTGA TATGGCAAAG 
GATGAAGAAT TATTAAAAGG CTACAAGGCT CTCGAGTTAA CCAGTGAAGA AGCATCAAAG 
AGCGGAGGGT CACTTTGGGC GCAGTGTCAA GCACTTGCAG ATATGAAGTT CACCTTTGTG 
GTTTCTTGTC AACAATACAG TATTCATAAG AGATCTGGCG ACCAACGTGC TAAAGACATA 
TTGAGACTTA TGACGACGTA TCCGTCTATT CGTGTCGCCT ATATCGATGA GGTAGAGCAA 
ACACATAAAG AAAGTTATAA GGGGACAGAG GAGAAAATTT ATTACTCAGC GCTTGTAAAA 
GCTGCTCCAC AGACCAAACC AATGGATTCT TCTGAATCTG TTCAAACACT AGATCAGCTC 
ATTTATCGGA TTAAGCTTCC GGGACCAGCT ATACTAGGAG AGGGAAAGCC GGAAAATCAG 
AACCATGCTA TCATATTTAC CCGTGGAGAA GGGTTGCAGA CGATAGACAT GAATCAGGAC 
AACTACATGG AGGAAGCTTT CAAAATGAGG AACTTGCTGC AAGAGTTTCT TGAAAAGCAT 
GGAGGCGTAA GATGTCCTAC GATTCTTGGT CTTAGAGAGC ATATTTTCAC TGGAAGTGTG 
TCTTCTCTTG CATGGTTTAT GTCAAATCAA GAGAACAGTT TTGTAACGAT TGGGCAAAGA 
GTGCTAGCTA GTCCCTTGAA AGTACGATTC CATTACGGAC ATCCAGATAT TTTTGATCGT 
CTGTTTCACC TTACCAGAGG TGGTATCTGC AAAGCTTCTA AAGTCATCAA TCTTAGTGAA 
GACATATTTG CAGGTTTTAA TTCTACTCTG CGTGAAGGAA ATGTGACTCA TCATGAATAT 
ATACAAGTTG GTAAAGGGAG AGATGTGGGC CTCAACCAGA TCTCAATGTT TGAGGCAAAA 
ATCGCCAATG GAAATGGCGA GCAAACTTTG AGTCGCGACC TTTATAGGCT AGGACACCGA 
TTTGATTTCT TCCGGATGCT GTCTTGTTAT TTCACCACAA TTGGGTTCTA CTTCAGTACC 
ATGTTAACCG TGCTTACGGT GTATGTGTTT CTTTATGGAC GGTTATATCT AGTACTTAGT 
GGGCTTGAAG AAGGATTGAG TAGCCAACGA GCTTTCCGTA ACAATAAGCC TCTTGAGGCA 
GCTCTTGCTT CTCAATCATT TGTGCAGATT GGTTTTTTAA TGGCTTTGCC TATGATGATG 
GAGATTGGAC TTGAGAGGGG GTTCCACAAT GCATTGATTG AGTTTGTGCT GATGCAGTTA 
CAACTTGCTT CCGTCTTTTT CACGTTTCAG CTCGGGACAA AAACACATTA CTACGGAAGG 
ACATTGTTTC ATGGAGGCGC AGAGTATAGA GGAACTGGTC GAGGGTTTGT GGTCTTCCAC 
GCGAAATTTG CTGAGAACTA TAGGTTCTAT TCCCGTAGTC ACTTTGTGAA GGGCATTGAA 
CTTATGATTC TTCTACTGGT ATATCAGATC TTTGGTCAAT CATATAGGGG TGTGGTTACT 
TATATTCTGA TCACTGTTTC GATATGGTTC ATGGTGGTGA CATGGCTCTT TGCTCCTTTC 
CTTTTCAATC CGTCTGGTTT TGAGTGGCAA AAGATTGTTG ATGACTGGAC AGACTGGAAC 
AAATGGATTT ATAACCGAGG AGGAATTGGT GTCCCTCCTG AGAAGAGTTG GGAATCTTGG 
TGGGAGAAAG AGTTAGAACA TCTTAGACAT TCAGGGGTTC GTGGTATCAC CCTTGAGATC 
TTTTTGGCCT TGCGCTTCTT CATCTTTCAA TATGGACTTG TGTATCATCT CAGTACCTTC 
AAAGGAAAAA ATCAGAGCTT TTGGGTTTAT GGAGCGTCAT GGTTTGTGAT CTTATTCATT 
CTACTGATTG TGAAGGGTTT GGGCGTTGGA AGGCGAAGAT TCAGCACGAA TTTCCAGCTT 
CTATTCAGAA TCATAAAGGG TCTTGTGTTC CTCACATTTG TGGCAATTCT CATAACTTTC 
CTCGCACTTC CTCTGATAAC AATCAAAGAC TTATTCATCT GCATGCTTGC CTTCATGCCA 
ACTGGATGGG GAATGCTTCT CATTGCACAA GCGTGTAAGC CTCTGATCCA ACAGCTAGGG 
ATATGGTCAT CAGTTAGGAC GCTAGCTCGA GGCTACGAGA TCGTGATGGG CTTGCTTCTG 
TTCACACCAG TTGCGTTTTT GGCCTGGTTT CCTTTTGTGT CTGAGTTCCA GACAAGAATG 
CTCTTTAACC AAGCCTTTAG TAGAGGTCTA CAAATCTCTC GTATTCTTGG TGGTCAAAGA 
AAAGACCGGT CTTCAAAAAA CAAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184913.1
CDS	205..5934
Translation	MAQRREPDPPPPQRRILRTQTVGSLGEAMLDSEVVPSSLVEIAPILRVANEVEASNPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALLQRLERENETTLAGRQKSDAREMQSFYQHYYKKYIQALLNAADKADRAQLTKAYQTAAVLFEVLKAVNQTEDVEVADEILETHNKVEEKTQIYVPYNILPLDPDSQNQAIMRLPEIQAAVAALRNTRGLPWTAGHKKKLDEDILDWLQSMFGFQKDNVLNQREHLILLLANVHIRQFPKPDQQPKLDDRALTIVMKKLFRNYKKWCKYLGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGSVSPMTGEHVKPAYGGEDEAFLQKVVTPIYQTISKEAKRSRGGKSKHSVWRNYDDLNEYFWSIRCFRLGWPMRADADFFCQTAEELRLERSEIKSNSGDRWMGKVNFVEIRSFWHIFRSFDRLWSFYILCLQAMIVIAWNGSGELSAIFQGDVFLKVLSVFITAAILKLAQAVLDIALSWKARHSMSLYVKLRYVMKVGAAAVWVVVMAVTYAYSWKNASGFSQTIKNWSDYKIMMLMMWWSQPRLYIGRGMHESALSLFKYTMFWIVLLISKLAFSYYAEIKPLVGPTKDIMRIHISVYSWHEFFPHAKNNLGVVIALWSPVILVYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSIPGAFNDCLVPQDNSDDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISSFREEDLISDREMELLLVPYWSDPDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELKKRLAVDSYMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALPDLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLERVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEELLKGYKALELTSEEASKSGGSLWAQCQALADMKFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYKGTEEKIYYSALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE

Target ORF information:

RefSeq Version	NM_001197984.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana callose synthase 1 (CALS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197984.1

ORF Insert Sequence:

ATGGCTCAAA GAAGGGAACC TGATCCGCCT CCACCGCAGA GGCGGATTCT GCGGACTCAG 
ACTGTTGGTA GTCTCGGAGA GGCTATGCTT GATAGTGAAG TTGTTCCCTC TTCTTTAGTT 
GAGATTGCTC CGATTCTACG TGTGGCTAAT GAAGTTGAAG CTAGTAATCC ACGAGTTGCT 
TACCTATGTC GTTTCTACGC ATTTGAGAAG GCGCATAGGC TTGATCCAAC TTCAAGTGGT 
CGTGGTGTTC GCCAGTTTAA GACCGCGCTT CTTCAACGAT TAGAAAGGGA GAATGAAACA 
ACTTTGGCAG GAAGGCAAAA GAGTGATGCA CGTGAAATGC AGAGTTTCTA TCAACACTAC 
TACAAGAAAT ACATCCAAGC TTTGCTGAAT GCTGCGGATA AAGCTGACCG TGCTCAACTT 
ACAAAGGCCT ATCAAACTGC TGCCGTTCTC TTTGAAGTCT TGAAGGCTGT TAATCAAACA 
GAAGATGTGG AAGTGGCTGA TGAGATTTTG GAAACGCACA ATAAGGTGGA AGAGAAGACC 
CAGATTTACG TGCCTTACAA TATACTTCCC CTTGACCCTG ATAGTCAGAA TCAGGCAATA 
ATGAGATTGC CTGAGATACA AGCTGCTGTA GCCGCTCTTA GAAATACAAG GGGATTACCA 
TGGACAGCAG GTCACAAGAA GAAACTCGAT GAAGACATTC TAGACTGGCT TCAGTCTATG 
TTTGGTTTTC AGAAAGATAA TGTTTTGAAT CAAAGAGAGC ACTTGATCTT GTTACTTGCT 
AATGTCCACA TCCGACAATT TCCAAAACCT GACCAACAAC CAAAGTTGGA TGATCGTGCC 
TTGACCATAG TGATGAAGAA ACTTTTCAGA AACTACAAAA AGTGGTGCAA ATACTTGGGT 
CGGAAAAGCA GCCTTTGGTT GCCTACTATT CAGCAAGAGG TACAGCAGCG TAAACTGCTC 
TACATGGGAC TTTATCTATT AATATGGGGA GAAGCTGCAA ACCTGAGATT CATGCCAGAA 
TGCCTCTGTT ATATATATCA CCATATGGCT TTCGAGCTTT ATGGTATGTT GGCCGGTAGT 
GTTAGTCCCA TGACAGGGGA GCATGTAAAG CCAGCGTATG GTGGTGAAGA TGAAGCTTTC 
CTGCAGAAAG TTGTGACTCC TATATACCAG ACAATATCAA AGGAAGCAAA GAGAAGTAGA 
GGAGGAAAGT CAAAGCATTC TGTGTGGAGA AACTATGATG ACTTGAATGA ATATTTTTGG 
TCAATTCGAT GTTTTCGCTT AGGGTGGCCA ATGCGGGCTG ATGCTGATTT CTTTTGTCAG 
ACAGCTGAAG AACTTCGACT TGAGAGAAGT GAGATAAAAT CAAACAGTGG AGATCGGTGG 
ATGGGCAAAG TCAATTTTGT GGAGATACGC TCGTTCTGGC ATATATTTAG AAGTTTTGAT 
AGATTGTGGA GCTTTTATAT CCTGTGTTTG CAGGCAATGA TTGTTATTGC ATGGAATGGA 
TCCGGAGAGT TAAGCGCCAT TTTTCAGGGT GATGTCTTCC TAAAGGTTTT GAGCGTCTTC 
ATTACAGCAG CTATACTAAA GCTTGCACAA GCTGTCCTTG ACATAGCCCT CAGCTGGAAG 
GCAAGACATA GTATGTCACT CTATGTGAAA CTCAGATACG TCATGAAGGT AGGTGCGGCT 
GCAGTATGGG TTGTAGTCAT GGCAGTTACT TATGCGTACA GCTGGAAAAA CGCCTCCGGT 
TTTTCTCAGA CCATAAAGAA TTGGTCTGAC TATAAGATAA TGATGCTGAT GATGTGGTGG 
TCTCAGCCTC GTCTGTATAT AGGAAGGGGT ATGCATGAGA GCGCTTTGTC ACTTTTCAAG 
TACACAATGT TTTGGATCGT TCTGCTGATT TCAAAGCTGG CATTCAGTTA TTACGCTGAG 
ATTAAGCCCC TTGTTGGTCC AACCAAAGAT ATAATGCGAA TCCATATATC CGTCTACAGC 
TGGCATGAGT TTTTCCCTCA CGCCAAGAAT AATTTAGGAG TCGTCATCGC GCTCTGGTCA 
CCAGTTATTC TCGTTTATTT CATGGACACT CAAATATGGT ATGCTATAGT TTCAACTCTG 
GTCGGAGGTT TAAATGGAGC TTTCCGCCGT CTTGGAGAGA TCCGAACACT TGGAATGTTG 
AGGTCGAGAT TTCAGTCCAT ACCAGGGGCC TTTAATGATT GCTTGGTACC ACAAGATAAC 
AGCGATGATA CAAAGAAAAA GAGATTTAGG GCGACCTTCT CTCGCAAGTT TGATCAGCTC 
CCATCTAGTA AAGACAAGGA AGCAGCGCGG TTTGCTCAGA TGTGGAACAA AATAATCAGT 
AGTTTCCGAG AAGAGGACTT GATTAGTGAT AGGGAAATGG AACTATTGCT TGTGCCATAT 
TGGTCTGACC CTGATTTGGA TCTTATCCGG TGGCCACCTT TTCTATTGGC TAGTAAGATC 
CCAATAGCTT TGGATATGGC TAAAGACAGC AATGGAAAAG ACCGTGAACT GAAAAAGAGA 
TTAGCTGTTG ACAGCTACAT GACTTGTGCA GTTAGAGAGT GCTATGCCTC TTTTAAGAAT 
CTCATCAATT ATTTGGTGGT CGGAGAACGA GAAGGACAGG TCATAAACGA CATTTTCTCC 
AAGATTGATG AACATATTGA GAAGGAAACG CTGATAACAG AATTGAACCT GAGTGCTCTT 
CCTGATCTGT ATGGCCAGTT CGTTCGGCTA ATCGAATATT TGCTAGAAAA CAGGGAGGAG 
GACAAGGATC AAATTGTAAT TGTCTTGCTG AACATGTTAG AATTGGTGAC TAGAGACATC 
ATGGAAGAAG AAGTCCCAAG CTTACTAGAG ACTGCCCACA ATGGATCTTA TGTCAAGTAT 
GACGTCATGA CCCCTCTTCA TCAACAGCGC AAATATTTCA GTCAACTTCG ATTCCCAGTT 
TATTCTCAAA CGGAAGCTTG GAAAGAAAAA ATTAAGAGGC TTCATCTTTT GCTTACTGTC 
AAGGAGTCAG CTATGGATGT TCCATCCAAC TTGGAAGCTC GAAGGCGTCT TACTTTCTTT 
TCAAATTCAC TATTCATGGA CATGCCTCCT GCACCCAAGA TCCGCAACAT GCTTTCCTTC 
TCGGTCCTTA CACCATACTT TTCAGAGGAT GTTCTTTTCT CCATATTTGG CTTAGAACAG 
CAAAATGAAG ATGGAGTTTC TATACTCTTT TACTTGCAGA AGATCTTCCC AGATGAATGG 
ACAAACTTTC TGGAACGAGT TAAATGTGGC AACGAAGAAG AACTAAGAGC AAGAGAGGAT 
TTGGAAGAGG AGCTTCGTCT TTGGGCATCT TACAGAGGCC AAACTCTGAC TAAAACTGTG 
CGAGGAATGA TGTATTACCG AAAGGCTTTG GAACTTCAGG CCTTCCTTGA TATGGCAAAG 
GATGAAGAAT TATTAAAAGG CTACAAGGCT CTCGAGTTAA CCAGTGAAGA AGCATCAAAG 
AGCGGAGGGT CACTTTGGGC GCAGTGTCAA GCACTTGCAG ATATGAAGTT CACCTTTGTG 
GTTTCTTGTC AACAATACAG TATTCATAAG AGATCTGGCG ACCAACGTGC TAAAGACATA 
TTGAGACTTA TGACGACGTA TCCGTCTATT CGTGTCGCCT ATATCGATGA GGTAGAGCAA 
ACACATAAAG AAAGTTATAA GGGGACAGAG GAGAAAATTT ATTACTCAGC GCTTGTAAAA 
GCTGCTCCAC AGACCAAACC AATGGATTCT TCTGAATCTG TTCAAACACT AGATCAGCTC 
ATTTATCGGA TTAAGCTTCC GGGACCAGCT ATACTAGGAG AGGGAAAGCC GGAAAATCAG 
AACCATGCTA TCATATTTAC CCGTGGAGAA GGGTTGCAGA CGATAGACAT GAATCAGGAC 
AACTACATGG AGGAAGCTTT CAAAATGAGG AACTTGCTGC AAGAGTTTCT TGAAAAGCAT 
GGAGGCGTAA GATGTCCTAC GATTCTTGGT CTTAGAGAGC ATATTTTCAC TGGAAGTGTG 
TCTTCTCTTG CATGGTTTAT GTCAAATCAA GAGAACAGTT TTGTAACGAT TGGGCAAAGA 
GTGCTAGCTA GTCCCTTGAA AGTACGATTC CATTACGGAC ATCCAGATAT TTTTGATCGT 
CTGTTTCACC TTACCAGAGG TGGTATCTGC AAAGCTTCTA AAGTCATCAA TCTTAGTGAA 
GACATATTTG CAGGTTTTAA TTCTACTCTG CGTGAAGGAA ATGTGACTCA TCATGAATAT 
ATACAAGTTG GTAAAGGGAG AGATGTGGGC CTCAACCAGA TCTCAATGTT TGAGGCAAAA 
ATCGCCAATG GAAATGGCGA GCAAACTTTG AGTCGCGACC TTTATAGGCT AGGACACCGA 
TTTGATTTCT TCCGGATGCT GTCTTGTTAT TTCACCACAA TTGGGTTCTA CTTCAGTACC 
ATGTTAACCG TGCTTACGGT GTATGTGTTT CTTTATGGAC GGTTATATCT AGTACTTAGT 
GGGCTTGAAG AAGGATTGAG TAGCCAACGA GCTTTCCGTA ACAATAAGCC TCTTGAGGCA 
GCTCTTGCTT CTCAATCATT TGTGCAGATT GGTTTTTTAA TGGCTTTGCC TATGATGATG 
GAGATTGGAC TTGAGAGGGG GTTCCACAAT GCATTGATTG AGTTTGTGCT GATGCAGTTA 
CAACTTGCTT CCGTCTTTTT CACGTTTCAG CTCGGGACAA AAACACATTA CTACGGAAGG 
ACATTGTTTC ATGGAGGCGC AGAGTATAGA GGAACTGGTC GAGGGTTTGT GGTCTTCCAC 
GCGAAATTTG CTGAGAACTA TAGGTTCTAT TCCCGTAGTC ACTTTGTGAA GGGCATTGAA 
CTTATGATTC TTCTACTGGT ATATCAGATC TTTGGTCAAT CATATAGGGG TGTGGTTACT 
TATATTCTGA TCACTGTTTC GATATGGTTC ATGGTGGTGA CATGGCTCTT TGCTCCTTTC 
CTTTTCAATC CGTCTGGTTT TGAGTGGCAA AAGATTGTTG ATGACTGGAC AGACTGGAAC 
AAATGGATTT ATAACCGAGG AGGAATTGGT GTCCCTCCTG AGAAGAGTTG GGAATCTTGG 
TGGGAGAAAG AGTTAGAACA TCTTAGACAT TCAGGGGTTC GTGGTATCAC CCTTGAGATC 
TTTTTGGCCT TGCGCTTCTT CATCTTTCAA TATGGACTTG TGTATCATCT CAGTACCTTC 
AAAGGAAAAA ATCAGAGCTT TTGGGTTTAT GGAGCGTCAT GGTTTGTGAT CTTATTCATT 
CTACTGATTG TGAAGGGTTT GGGCGTTGGA AGGCGAAGAT TCAGCACGAA TTTCCAGCTT 
CTATTCAGAA TCATAAAGGG TCTTGTGTTC CTCACATTTG TGGCAATTCT CATAACTTTC 
CTCGCACTTC CTCTGATAAC AATCAAAGAC TTATTCATCT GCATGCTTGC CTTCATGCCA 
ACTGGATGGG GAATGCTTCT CATTGCACAA GCGTGTAAGC CTCTGATCCA ACAGCTAGGG 
ATATGGTCAT CAGTTAGGAC GCTAGCTCGA GGCTACGAGA TCGTGATGGG CTTGCTTCTG 
TTCACACCAG TTGCGTTTTT GGCCTGGTTT CCTTTTGTGT CTGAGTTCCA GACAAGAATG 
CTCTTTAACC AAGCCTTTAG TAGAGGTCTA CAAATCTCTC GTATTCTTGG TGGTCAAAGA 
AAAGACCGGT CTTCAAAAAA CAAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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CALS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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