VLN5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	VLN5
Entrez Gene ID	835837
Full Name	actin filament bundling protein P-115-ABP protein
Synonyms	MJB24.13,MJB24_13,villin 5
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001345256.1	NP_001318818.1	actin filament bundling protein P-115-ABP protein
NM_001345257.1	NP_001332290.1	actin filament bundling protein P-115-ABP protein

Pathways from BioSystems

Homologies

	VIL1	XP_001090524.1
Bos taurus (cattle)	VIL1	NP_001013609.1
Mus musculus (house mouse)	Vil1	NP_033535.2
Arabidopsis thaliana (thale cress)	AT5G57320	NP_200542.1
Arabidopsis thaliana (thale cress)	VLN4	NP_194745.1
Xenopus tropicalis (tropical clawed frog)	vil1	NP_001096367.1
Gallus gallus (chicken)	VIL1	NP_990773.1
Homo sapiens (human)	VIL1	NP_009058.2
Pan troglodytes (chimpanzee)	VIL1	XP_001157250.2
Danio rerio (zebrafish)	vil1	NP_956532.1
Oryza sativa (rice)	Os08g0240800	NP_001061335.1
Oryza sativa (rice)	Os06g0659300	NP_001058263.1
Canis lupus familiaris (dog)	VIL1	XP_545642.2
Rattus norvegicus (Norway rat)	Vil1	NP_001101694.1
Oryza sativa (rice)	Os04g0604000	NP_001053784.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that the construction of actin collars was affected in vln2 vln5 double mutantpollen tubes.
Title: Arabidopsis villins promote actin turnover at pollen tube tips and facilitate the construction of actin collars.

VLN5 is a major actin filament stabilizing factor as well as a regulator of actin dynamics that functions in concert with oscillatory Ca2+ gradients and regulates pollen tube growth.
Title: Arabidopsis VILLIN5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth.

The following VLN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VLN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb21675	NM_001345257.1 Latest version!	Arabidopsis thaliana actin filament bundling protein P-115-ABP protein (VLN5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb21675	NM_001345256.1 Latest version!	Arabidopsis thaliana actin filament bundling protein P-115-ABP protein (VLN5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VLN5 ( NM_001345256.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001318818.1 Arabidopsis thaliana actin filament bundling protein P-115-ABP protein (VLN5), partial mRNA.

Price & Availability↑

CloneID	OAb21675
Clone ID Related Accession (Same CDS sequence)	NM_001345256.1 , NM_001345257.1
Accession Version	NM_001345256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	actin filament bundling protein P-115-ABP protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). COMPLETENESS: incomplete on the 5' end.

ATGACGTTTT CCATGAGAGA TTTAGATCAG GCTCTTCAAG GAGCTGGCCA GAAATCCGGG 
ATTGAAATAT GGCGCATCGA GAACTTCAAA CCTGTCACTG TTCCACAAGA GTCTCATGGG 
AAATTTTTCA CCGGGGATTC CTATATAGTC TTAAAGACCA CAGCGTCAAG AAGCGGTTCT 
TTGCATCATG ATATTCACTA CTGGCTCGGT AAAGATTCCA GCCAGGATGA AGCAGGTGCT 
GTAGCCGTCA TGACTGTTGA ATTAGATTCA GCATTAGGTG GACGTGCTGT TCAGTACCGA 
GAAGTACAGG GTCACGAAAC CGAGAAGTTT CTTTCCTACT TCAAACCTTG CATAATACCT 
CAAGAAGGTG GAGTTGCTTC AGGGTTCAAC CATGTAAAGC CCGAGGAGCA TCAGACGCGT 
CTGTATATCT GCAAAGGCAA ACATGTCGTC CGTGTTAAAG AGGTTCCGTT TGTTCGATCG 
ACTCTCAACC ACGAAGACGT TTTTATTCTT GATACAGAGT CTAAAATTTT TCAATTCAGT 
GGTTCCAAGT CGAGTATTCA AGAAAGAGCA AAAGCTCTTG AGGTTGTTCA GTACATTAAA 
GACACTTACC ATGATGGAAA GTGCGATATC GCAGCTGTTG AGGATGGGAG GATGATGGCT 
GATGCTGAAG CTGGAGAGTT TTGGGGCTTG TTTGGTGGGT TTGCTCCGCT TCCTAAGAAA 
CCAGCAGTCA ATGATGACGA AACCGCTGCA TCTGATGGTA TCAAACTTTT CAGTGTCGAG 
AAGGGACAGA CAGATGCCGT AGAGGCTGAG TGTTTGACGA AAGAGCTTCT GGACACTAAC 
AAATGTTATA TTCTCGATTG CGGTCTTGAA TTGTTCGTTT GGAAGGGACG AAGTACTTCA 
ATCGACCAAA GAAAGAGCGC AACTGAAGCT GCAGAAGAAT TTTTCCGTTC GTCTGAACCG 
CCAAAATCAA ACCTGGTCAG TGTGATGGAA GGGTATGAAA CAGTGATGTT CCGATCTAAG 
TTTGATTCAT GGCCGGCTTC AAGTACCATA GCTGAGCCCC AACAAGGCAG AGGCAAAGTC 
GCAGCTCTTT TGCAGCGGCA AGGAGTTAAC GTTCAAGGCC TGGTTAAGAC TTCTTCTTCT 
TCTTCTAAAG ACGAACCGAA GCCATACATT GATGGTACAG GAAATCTCCA GGTCTGGCGA 
ATCAATTGTG AAGAAAAGAT CCTTCTCGAA GCAGCAGAGC AATCAAAGTT CTATAGCGGG 
GATTGTTATA TACTCCAGTA CTCATACCCA GGAGAAGACA GAGAGGAACA TCTAGTGGGT 
ACTTGGTTTG GCAAGCAAAG CGTTGAGGAA GACAGAGCAT CTGCTATTTC TTTGGCAAAC 
AAGATGGTTG AATCCATGAA GTTTGTGCCG GCTCAGGCTC GCATTAATGA GGGAAAAGAG 
CCAATTCAGT TTTTTGTGAT CATGCAAAGC TTCATCACAT TTAAGGGTGG TGTAAGTGAT 
GCTTTCAAAA AGTACATAGC TGAGAACGAC ATCCCTGATA CTACTTACGA AGCAGAAGGT 
GTTGCACTGT TTCGGGTTCA AGGTTCTGGA CCCGAGAACA TGCAAGCCAT ACAGATAGAA 
GCGGCTTCCG CAGGACTAAA TTCTTCGCAC TGTTATATAT TACACGGTGA TTCTACTGTT 
TTCACTTGGT GTGGCAATCT TACTTCCTCG GAAGATCAAG AACTTATGGA AAGAATGTTG 
GATTTGATTA AGCCAAATGA ACCCACTAAG GCACAAAAGG AAGGCTCAGA GTCTGAACAG 
TTTTGGGAAT TATTAGGAGG CAAATCAGAA TATCCGAGCC AAAAGATCAA AAGGGATGGA 
GAGAGTGATC CTCATCTCTT TTCTTGCACA TATACAAACG AAAGTCTGAA GGCTACAGAG 
ATCTTCAACT TCACTCAAGA TGATTTGATG ACTGAAGATA TCTTCATACT TGATTGTCAC 
ACTGAGGTCT TTGTCTGGGT GGGACAACAA GTTGACCCAA AGAAGAAACC TCAAGCTTTG 
GACATTGGAG AGAACTTTCT TAAGCATGAT TTCCTCCTAG AGAATCTAGC AAGTGAAACA 
CCGATATACA TTGTAACAGA GGGAAACGAA CCTCCATTTT TCACTCGGTT CTTCACCTGG 
GACTCTTCTA AATCTGGAAT GCATGGAGAC TCTTTCCAGA GAAAGCTTGC GATCCTGACA 
AATAAAGGAA AGCCGCTTCT AGATAAACCT AAAAGGAGAG TCCCTGCATA CAGTAGCAGG 
TCCACCGTTC CAGACAAATC GCAGCCTCGG TCCAGAAGTA TGACTTTTAG TCCAGACAGG 
GCCCGCGTGA GGGGACGGTC TCCAGCTTTC AACGCACTTG CAGCAAACTT TGAGAAACTA 
AATATAAGAA ACCAATCAAC TCCACCACCT ATGGTTAGTC CAATGGTCCG GAAGCTTTAC 
CCGAAATCTC ATGCCCCAGA CCTCTCGAAG ATAGCTCCTA AATCCGCTAT AGCTGCCCGT 
ACAGCGCTTT TTGAAAAACC TACTCCTACT TCTCAAGAAC CACCTACTAG CCCCAGTTCT 
TCAGAAGCTA CAAACCAAGC AGAAGCACCA AAATCGACAT CAGAGACAAA CGAGGAAGAA 
GCAATGAGCA GCATCAATGA AGACTCAAAA GAAGAAGAAG CAGAAGAAGA GAGCAGCCTC 
CCTACTTTCC CATATGAACG CCTTAAAACC GACTCAGAGG ATCCTGTTTC AGATGTCGAC 
CTCACTAGAA GAGAGGCATA CTTGACTTCA GTAGAGTTCA AGGAGAAGTT TGAGATGACA 
AAGAATGAGT TCTACAAACT GCCTAAATGG AAACAAAACA AGCTCAAAAT GTCTGTCAAT 
CTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318818.1
CDS	1..2889
Translation	MTFSMRDLDQALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLGKDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFNHVKPEEHQTRLYICKGKHVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQERAKALEVVQYIKDTYHDGKCDIAAVEDGRMMADAEAGEFWGLFGGFAPLPKKPAVNDDETAASDGIKLFSVEKGQTDAVEAECLTKELLDTNKCYILDCGLELFVWKGRSTSIDQRKSATEAAEEFFRSSEPPKSNLVSVMEGYETVMFRSKFDSWPASSTIAEPQQGRGKVAALLQRQGVNVQGLVKTSSSSSKDEPKPYIDGTGNLQVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFGKQSVEEDRASAISLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITFKGGVSDAFKKYIAENDIPDTTYEAEGVALFRVQGSGPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCGNLTSSEDQELMERMLDLIKPNEPTKAQKEGSESEQFWELLGGKSEYPSQKIKRDGESDPHLFSCTYTNESLKATEIFNFTQDDLMTEDIFILDCHTEVFVWVGQQVDPKKKPQALDIGENFLKHDFLLENLASETPIYIVTEGNEPPFFTRFFTWDSSKSGMHGDSFQRKLAILTNKGKPLLDKPKRRVPAYSSRSTVPDKSQPRSRSMTFSPDRARVRGRSPAFNALAANFEKLNIRNQSTPPPMVSPMVRKLYPKSHAPDLSKIAPKSAIAARTALFEKPTPTSQEPPTSPSSSEATNQAEAPKSTSETNEEEAMSSINEDSKEEEAEEESSLPTFPYERLKTDSEDPVSDVDLTRREAYLTSVEFKEKFEMTKNEFYKLPKWKQNKLKMSVNLF

Target ORF information:

RefSeq Version	NM_001345256.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana actin filament bundling protein P-115-ABP protein (VLN5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001345256.1

ORF Insert Sequence:

ATGACGTTTT CCATGAGAGA TTTAGATCAG GCTCTTCAAG GAGCTGGCCA GAAATCCGGG 
ATTGAAATAT GGCGCATCGA GAACTTCAAA CCTGTCACTG TTCCACAAGA GTCTCATGGG 
AAATTTTTCA CCGGGGATTC CTATATAGTC TTAAAGACCA CAGCGTCAAG AAGCGGTTCT 
TTGCATCATG ATATTCACTA CTGGCTCGGT AAAGATTCCA GCCAGGATGA AGCAGGTGCT 
GTAGCCGTCA TGACTGTTGA ATTAGATTCA GCATTAGGTG GACGTGCTGT TCAGTACCGA 
GAAGTACAGG GTCACGAAAC CGAGAAGTTT CTTTCCTACT TCAAACCTTG CATAATACCT 
CAAGAAGGTG GAGTTGCTTC AGGGTTCAAC CATGTAAAGC CCGAGGAGCA TCAGACGCGT 
CTGTATATCT GCAAAGGCAA ACATGTCGTC CGTGTTAAAG AGGTTCCGTT TGTTCGATCG 
ACTCTCAACC ACGAAGACGT TTTTATTCTT GATACAGAGT CTAAAATTTT TCAATTCAGT 
GGTTCCAAGT CGAGTATTCA AGAAAGAGCA AAAGCTCTTG AGGTTGTTCA GTACATTAAA 
GACACTTACC ATGATGGAAA GTGCGATATC GCAGCTGTTG AGGATGGGAG GATGATGGCT 
GATGCTGAAG CTGGAGAGTT TTGGGGCTTG TTTGGTGGGT TTGCTCCGCT TCCTAAGAAA 
CCAGCAGTCA ATGATGACGA AACCGCTGCA TCTGATGGTA TCAAACTTTT CAGTGTCGAG 
AAGGGACAGA CAGATGCCGT AGAGGCTGAG TGTTTGACGA AAGAGCTTCT GGACACTAAC 
AAATGTTATA TTCTCGATTG CGGTCTTGAA TTGTTCGTTT GGAAGGGACG AAGTACTTCA 
ATCGACCAAA GAAAGAGCGC AACTGAAGCT GCAGAAGAAT TTTTCCGTTC GTCTGAACCG 
CCAAAATCAA ACCTGGTCAG TGTGATGGAA GGGTATGAAA CAGTGATGTT CCGATCTAAG 
TTTGATTCAT GGCCGGCTTC AAGTACCATA GCTGAGCCCC AACAAGGCAG AGGCAAAGTC 
GCAGCTCTTT TGCAGCGGCA AGGAGTTAAC GTTCAAGGCC TGGTTAAGAC TTCTTCTTCT 
TCTTCTAAAG ACGAACCGAA GCCATACATT GATGGTACAG GAAATCTCCA GGTCTGGCGA 
ATCAATTGTG AAGAAAAGAT CCTTCTCGAA GCAGCAGAGC AATCAAAGTT CTATAGCGGG 
GATTGTTATA TACTCCAGTA CTCATACCCA GGAGAAGACA GAGAGGAACA TCTAGTGGGT 
ACTTGGTTTG GCAAGCAAAG CGTTGAGGAA GACAGAGCAT CTGCTATTTC TTTGGCAAAC 
AAGATGGTTG AATCCATGAA GTTTGTGCCG GCTCAGGCTC GCATTAATGA GGGAAAAGAG 
CCAATTCAGT TTTTTGTGAT CATGCAAAGC TTCATCACAT TTAAGGGTGG TGTAAGTGAT 
GCTTTCAAAA AGTACATAGC TGAGAACGAC ATCCCTGATA CTACTTACGA AGCAGAAGGT 
GTTGCACTGT TTCGGGTTCA AGGTTCTGGA CCCGAGAACA TGCAAGCCAT ACAGATAGAA 
GCGGCTTCCG CAGGACTAAA TTCTTCGCAC TGTTATATAT TACACGGTGA TTCTACTGTT 
TTCACTTGGT GTGGCAATCT TACTTCCTCG GAAGATCAAG AACTTATGGA AAGAATGTTG 
GATTTGATTA AGCCAAATGA ACCCACTAAG GCACAAAAGG AAGGCTCAGA GTCTGAACAG 
TTTTGGGAAT TATTAGGAGG CAAATCAGAA TATCCGAGCC AAAAGATCAA AAGGGATGGA 
GAGAGTGATC CTCATCTCTT TTCTTGCACA TATACAAACG AAAGTCTGAA GGCTACAGAG 
ATCTTCAACT TCACTCAAGA TGATTTGATG ACTGAAGATA TCTTCATACT TGATTGTCAC 
ACTGAGGTCT TTGTCTGGGT GGGACAACAA GTTGACCCAA AGAAGAAACC TCAAGCTTTG 
GACATTGGAG AGAACTTTCT TAAGCATGAT TTCCTCCTAG AGAATCTAGC AAGTGAAACA 
CCGATATACA TTGTAACAGA GGGAAACGAA CCTCCATTTT TCACTCGGTT CTTCACCTGG 
GACTCTTCTA AATCTGGAAT GCATGGAGAC TCTTTCCAGA GAAAGCTTGC GATCCTGACA 
AATAAAGGAA AGCCGCTTCT AGATAAACCT AAAAGGAGAG TCCCTGCATA CAGTAGCAGG 
TCCACCGTTC CAGACAAATC GCAGCCTCGG TCCAGAAGTA TGACTTTTAG TCCAGACAGG 
GCCCGCGTGA GGGGACGGTC TCCAGCTTTC AACGCACTTG CAGCAAACTT TGAGAAACTA 
AATATAAGAA ACCAATCAAC TCCACCACCT ATGGTTAGTC CAATGGTCCG GAAGCTTTAC 
CCGAAATCTC ATGCCCCAGA CCTCTCGAAG ATAGCTCCTA AATCCGCTAT AGCTGCCCGT 
ACAGCGCTTT TTGAAAAACC TACTCCTACT TCTCAAGAAC CACCTACTAG CCCCAGTTCT 
TCAGAAGCTA CAAACCAAGC AGAAGCACCA AAATCGACAT CAGAGACAAA CGAGGAAGAA 
GCAATGAGCA GCATCAATGA AGACTCAAAA GAAGAAGAAG CAGAAGAAGA GAGCAGCCTC 
CCTACTTTCC CATATGAACG CCTTAAAACC GACTCAGAGG ATCCTGTTTC AGATGTCGAC 
CTCACTAGAA GAGAGGCATA CTTGACTTCA GTAGAGTTCA AGGAGAAGTT TGAGATGACA 
AAGAATGAGT TCTACAAACT GCCTAAATGG AAACAAAACA AGCTCAAAAT GTCTGTCAAT 
CTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VLN5 ( NM_001345257.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001332290.1 Arabidopsis thaliana actin filament bundling protein P-115-ABP protein (VLN5), mRNA.

Price & Availability↑

CloneID	OAb21675
Clone ID Related Accession (Same CDS sequence)	NM_001345256.1 , NM_001345257.1
Accession Version	NM_001345257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	actin filament bundling protein P-115-ABP protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGACGTTTT CCATGAGAGA TTTAGATCAG GCTCTTCAAG GAGCTGGCCA GAAATCCGGG 
ATTGAAATAT GGCGCATCGA GAACTTCAAA CCTGTCACTG TTCCACAAGA GTCTCATGGG 
AAATTTTTCA CCGGGGATTC CTATATAGTC TTAAAGACCA CAGCGTCAAG AAGCGGTTCT 
TTGCATCATG ATATTCACTA CTGGCTCGGT AAAGATTCCA GCCAGGATGA AGCAGGTGCT 
GTAGCCGTCA TGACTGTTGA ATTAGATTCA GCATTAGGTG GACGTGCTGT TCAGTACCGA 
GAAGTACAGG GTCACGAAAC CGAGAAGTTT CTTTCCTACT TCAAACCTTG CATAATACCT 
CAAGAAGGTG GAGTTGCTTC AGGGTTCAAC CATGTAAAGC CCGAGGAGCA TCAGACGCGT 
CTGTATATCT GCAAAGGCAA ACATGTCGTC CGTGTTAAAG AGGTTCCGTT TGTTCGATCG 
ACTCTCAACC ACGAAGACGT TTTTATTCTT GATACAGAGT CTAAAATTTT TCAATTCAGT 
GGTTCCAAGT CGAGTATTCA AGAAAGAGCA AAAGCTCTTG AGGTTGTTCA GTACATTAAA 
GACACTTACC ATGATGGAAA GTGCGATATC GCAGCTGTTG AGGATGGGAG GATGATGGCT 
GATGCTGAAG CTGGAGAGTT TTGGGGCTTG TTTGGTGGGT TTGCTCCGCT TCCTAAGAAA 
CCAGCAGTCA ATGATGACGA AACCGCTGCA TCTGATGGTA TCAAACTTTT CAGTGTCGAG 
AAGGGACAGA CAGATGCCGT AGAGGCTGAG TGTTTGACGA AAGAGCTTCT GGACACTAAC 
AAATGTTATA TTCTCGATTG CGGTCTTGAA TTGTTCGTTT GGAAGGGACG AAGTACTTCA 
ATCGACCAAA GAAAGAGCGC AACTGAAGCT GCAGAAGAAT TTTTCCGTTC GTCTGAACCG 
CCAAAATCAA ACCTGGTCAG TGTGATGGAA GGGTATGAAA CAGTGATGTT CCGATCTAAG 
TTTGATTCAT GGCCGGCTTC AAGTACCATA GCTGAGCCCC AACAAGGCAG AGGCAAAGTC 
GCAGCTCTTT TGCAGCGGCA AGGAGTTAAC GTTCAAGGCC TGGTTAAGAC TTCTTCTTCT 
TCTTCTAAAG ACGAACCGAA GCCATACATT GATGGTACAG GAAATCTCCA GGTCTGGCGA 
ATCAATTGTG AAGAAAAGAT CCTTCTCGAA GCAGCAGAGC AATCAAAGTT CTATAGCGGG 
GATTGTTATA TACTCCAGTA CTCATACCCA GGAGAAGACA GAGAGGAACA TCTAGTGGGT 
ACTTGGTTTG GCAAGCAAAG CGTTGAGGAA GACAGAGCAT CTGCTATTTC TTTGGCAAAC 
AAGATGGTTG AATCCATGAA GTTTGTGCCG GCTCAGGCTC GCATTAATGA GGGAAAAGAG 
CCAATTCAGT TTTTTGTGAT CATGCAAAGC TTCATCACAT TTAAGGGTGG TGTAAGTGAT 
GCTTTCAAAA AGTACATAGC TGAGAACGAC ATCCCTGATA CTACTTACGA AGCAGAAGGT 
GTTGCACTGT TTCGGGTTCA AGGTTCTGGA CCCGAGAACA TGCAAGCCAT ACAGATAGAA 
GCGGCTTCCG CAGGACTAAA TTCTTCGCAC TGTTATATAT TACACGGTGA TTCTACTGTT 
TTCACTTGGT GTGGCAATCT TACTTCCTCG GAAGATCAAG AACTTATGGA AAGAATGTTG 
GATTTGATTA AGCCAAATGA ACCCACTAAG GCACAAAAGG AAGGCTCAGA GTCTGAACAG 
TTTTGGGAAT TATTAGGAGG CAAATCAGAA TATCCGAGCC AAAAGATCAA AAGGGATGGA 
GAGAGTGATC CTCATCTCTT TTCTTGCACA TATACAAACG AAAGTCTGAA GGCTACAGAG 
ATCTTCAACT TCACTCAAGA TGATTTGATG ACTGAAGATA TCTTCATACT TGATTGTCAC 
ACTGAGGTCT TTGTCTGGGT GGGACAACAA GTTGACCCAA AGAAGAAACC TCAAGCTTTG 
GACATTGGAG AGAACTTTCT TAAGCATGAT TTCCTCCTAG AGAATCTAGC AAGTGAAACA 
CCGATATACA TTGTAACAGA GGGAAACGAA CCTCCATTTT TCACTCGGTT CTTCACCTGG 
GACTCTTCTA AATCTGGAAT GCATGGAGAC TCTTTCCAGA GAAAGCTTGC GATCCTGACA 
AATAAAGGAA AGCCGCTTCT AGATAAACCT AAAAGGAGAG TCCCTGCATA CAGTAGCAGG 
TCCACCGTTC CAGACAAATC GCAGCCTCGG TCCAGAAGTA TGACTTTTAG TCCAGACAGG 
GCCCGCGTGA GGGGACGGTC TCCAGCTTTC AACGCACTTG CAGCAAACTT TGAGAAACTA 
AATATAAGAA ACCAATCAAC TCCACCACCT ATGGTTAGTC CAATGGTCCG GAAGCTTTAC 
CCGAAATCTC ATGCCCCAGA CCTCTCGAAG ATAGCTCCTA AATCCGCTAT AGCTGCCCGT 
ACAGCGCTTT TTGAAAAACC TACTCCTACT TCTCAAGAAC CACCTACTAG CCCCAGTTCT 
TCAGAAGCTA CAAACCAAGC AGAAGCACCA AAATCGACAT CAGAGACAAA CGAGGAAGAA 
GCAATGAGCA GCATCAATGA AGACTCAAAA GAAGAAGAAG CAGAAGAAGA GAGCAGCCTC 
CCTACTTTCC CATATGAACG CCTTAAAACC GACTCAGAGG ATCCTGTTTC AGATGTCGAC 
CTCACTAGAA GAGAGGCATA CTTGACTTCA GTAGAGTTCA AGGAGAAGTT TGAGATGACA 
AAGAATGAGT TCTACAAACT GCCTAAATGG AAACAAAACA AGCTCAAAAT GTCTGTCAAT 
CTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001332290.1
CDS	305..3193
Translation	MTFSMRDLDQALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLGKDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFNHVKPEEHQTRLYICKGKHVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQERAKALEVVQYIKDTYHDGKCDIAAVEDGRMMADAEAGEFWGLFGGFAPLPKKPAVNDDETAASDGIKLFSVEKGQTDAVEAECLTKELLDTNKCYILDCGLELFVWKGRSTSIDQRKSATEAAEEFFRSSEPPKSNLVSVMEGYETVMFRSKFDSWPASSTIAEPQQGRGKVAALLQRQGVNVQGLVKTSSSSSKDEPKPYIDGTGNLQVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFGKQSVEEDRASAISLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITFKGGVSDAFKKYIAENDIPDTTYEAEGVALFRVQGSGPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCGNLTSSEDQELMERMLDLIKPNEPTKAQKEGSESEQFWELLGGKSEYPSQKIKRDGESDPHLFSCTYTNESLKATEIFNFTQDDLMTEDIFILDCHTEVFVWVGQQVDPKKKPQALDIGENFLKHDFLLENLASETPIYIVTEGNEPPFFTRFFTWDSSKSGMHGDSFQRKLAILTNKGKPLLDKPKRRVPAYSSRSTVPDKSQPRSRSMTFSPDRARVRGRSPAFNALAANFEKLNIRNQSTPPPMVSPMVRKLYPKSHAPDLSKIAPKSAIAARTALFEKPTPTSQEPPTSPSSSEATNQAEAPKSTSETNEEEAMSSINEDSKEEEAEEESSLPTFPYERLKTDSEDPVSDVDLTRREAYLTSVEFKEKFEMTKNEFYKLPKWKQNKLKMSVNLF

Target ORF information:

RefSeq Version	NM_001345257.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana actin filament bundling protein P-115-ABP protein (VLN5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001345257.1

ORF Insert Sequence:

ATGACGTTTT CCATGAGAGA TTTAGATCAG GCTCTTCAAG GAGCTGGCCA GAAATCCGGG 
ATTGAAATAT GGCGCATCGA GAACTTCAAA CCTGTCACTG TTCCACAAGA GTCTCATGGG 
AAATTTTTCA CCGGGGATTC CTATATAGTC TTAAAGACCA CAGCGTCAAG AAGCGGTTCT 
TTGCATCATG ATATTCACTA CTGGCTCGGT AAAGATTCCA GCCAGGATGA AGCAGGTGCT 
GTAGCCGTCA TGACTGTTGA ATTAGATTCA GCATTAGGTG GACGTGCTGT TCAGTACCGA 
GAAGTACAGG GTCACGAAAC CGAGAAGTTT CTTTCCTACT TCAAACCTTG CATAATACCT 
CAAGAAGGTG GAGTTGCTTC AGGGTTCAAC CATGTAAAGC CCGAGGAGCA TCAGACGCGT 
CTGTATATCT GCAAAGGCAA ACATGTCGTC CGTGTTAAAG AGGTTCCGTT TGTTCGATCG 
ACTCTCAACC ACGAAGACGT TTTTATTCTT GATACAGAGT CTAAAATTTT TCAATTCAGT 
GGTTCCAAGT CGAGTATTCA AGAAAGAGCA AAAGCTCTTG AGGTTGTTCA GTACATTAAA 
GACACTTACC ATGATGGAAA GTGCGATATC GCAGCTGTTG AGGATGGGAG GATGATGGCT 
GATGCTGAAG CTGGAGAGTT TTGGGGCTTG TTTGGTGGGT TTGCTCCGCT TCCTAAGAAA 
CCAGCAGTCA ATGATGACGA AACCGCTGCA TCTGATGGTA TCAAACTTTT CAGTGTCGAG 
AAGGGACAGA CAGATGCCGT AGAGGCTGAG TGTTTGACGA AAGAGCTTCT GGACACTAAC 
AAATGTTATA TTCTCGATTG CGGTCTTGAA TTGTTCGTTT GGAAGGGACG AAGTACTTCA 
ATCGACCAAA GAAAGAGCGC AACTGAAGCT GCAGAAGAAT TTTTCCGTTC GTCTGAACCG 
CCAAAATCAA ACCTGGTCAG TGTGATGGAA GGGTATGAAA CAGTGATGTT CCGATCTAAG 
TTTGATTCAT GGCCGGCTTC AAGTACCATA GCTGAGCCCC AACAAGGCAG AGGCAAAGTC 
GCAGCTCTTT TGCAGCGGCA AGGAGTTAAC GTTCAAGGCC TGGTTAAGAC TTCTTCTTCT 
TCTTCTAAAG ACGAACCGAA GCCATACATT GATGGTACAG GAAATCTCCA GGTCTGGCGA 
ATCAATTGTG AAGAAAAGAT CCTTCTCGAA GCAGCAGAGC AATCAAAGTT CTATAGCGGG 
GATTGTTATA TACTCCAGTA CTCATACCCA GGAGAAGACA GAGAGGAACA TCTAGTGGGT 
ACTTGGTTTG GCAAGCAAAG CGTTGAGGAA GACAGAGCAT CTGCTATTTC TTTGGCAAAC 
AAGATGGTTG AATCCATGAA GTTTGTGCCG GCTCAGGCTC GCATTAATGA GGGAAAAGAG 
CCAATTCAGT TTTTTGTGAT CATGCAAAGC TTCATCACAT TTAAGGGTGG TGTAAGTGAT 
GCTTTCAAAA AGTACATAGC TGAGAACGAC ATCCCTGATA CTACTTACGA AGCAGAAGGT 
GTTGCACTGT TTCGGGTTCA AGGTTCTGGA CCCGAGAACA TGCAAGCCAT ACAGATAGAA 
GCGGCTTCCG CAGGACTAAA TTCTTCGCAC TGTTATATAT TACACGGTGA TTCTACTGTT 
TTCACTTGGT GTGGCAATCT TACTTCCTCG GAAGATCAAG AACTTATGGA AAGAATGTTG 
GATTTGATTA AGCCAAATGA ACCCACTAAG GCACAAAAGG AAGGCTCAGA GTCTGAACAG 
TTTTGGGAAT TATTAGGAGG CAAATCAGAA TATCCGAGCC AAAAGATCAA AAGGGATGGA 
GAGAGTGATC CTCATCTCTT TTCTTGCACA TATACAAACG AAAGTCTGAA GGCTACAGAG 
ATCTTCAACT TCACTCAAGA TGATTTGATG ACTGAAGATA TCTTCATACT TGATTGTCAC 
ACTGAGGTCT TTGTCTGGGT GGGACAACAA GTTGACCCAA AGAAGAAACC TCAAGCTTTG 
GACATTGGAG AGAACTTTCT TAAGCATGAT TTCCTCCTAG AGAATCTAGC AAGTGAAACA 
CCGATATACA TTGTAACAGA GGGAAACGAA CCTCCATTTT TCACTCGGTT CTTCACCTGG 
GACTCTTCTA AATCTGGAAT GCATGGAGAC TCTTTCCAGA GAAAGCTTGC GATCCTGACA 
AATAAAGGAA AGCCGCTTCT AGATAAACCT AAAAGGAGAG TCCCTGCATA CAGTAGCAGG 
TCCACCGTTC CAGACAAATC GCAGCCTCGG TCCAGAAGTA TGACTTTTAG TCCAGACAGG 
GCCCGCGTGA GGGGACGGTC TCCAGCTTTC AACGCACTTG CAGCAAACTT TGAGAAACTA 
AATATAAGAA ACCAATCAAC TCCACCACCT ATGGTTAGTC CAATGGTCCG GAAGCTTTAC 
CCGAAATCTC ATGCCCCAGA CCTCTCGAAG ATAGCTCCTA AATCCGCTAT AGCTGCCCGT 
ACAGCGCTTT TTGAAAAACC TACTCCTACT TCTCAAGAAC CACCTACTAG CCCCAGTTCT 
TCAGAAGCTA CAAACCAAGC AGAAGCACCA AAATCGACAT CAGAGACAAA CGAGGAAGAA 
GCAATGAGCA GCATCAATGA AGACTCAAAA GAAGAAGAAG CAGAAGAAGA GAGCAGCCTC 
CCTACTTTCC CATATGAACG CCTTAAAACC GACTCAGAGG ATCCTGTTTC AGATGTCGAC 
CTCACTAGAA GAGAGGCATA CTTGACTTCA GTAGAGTTCA AGGAGAAGTT TGAGATGACA 
AAGAATGAGT TCTACAAACT GCCTAAATGG AAACAAAACA AGCTCAAAAT GTCTGTCAAT 
CTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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VLN5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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