VIL1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	VIL1
Entrez Gene ID	396423
Full Name	villin 1
Synonyms	VIL
General protein information	Preferred Names villin 1 Names villin-1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205442.1	NP_990773.1	villin-1

Pathways from BioSystems

Homologies

	VIL1	XP_001090524.1
Bos taurus (cattle)	VIL1	NP_001013609.1
Mus musculus (house mouse)	Vil1	NP_033535.2
Arabidopsis thaliana (thale cress)	AT5G57320	NP_200542.1
Arabidopsis thaliana (thale cress)	VLN4	NP_194745.1
Xenopus tropicalis (tropical clawed frog)	vil1	NP_001096367.1
Gallus gallus (chicken)	VIL1	NP_990773.1
Homo sapiens (human)	VIL1	NP_009058.2
Pan troglodytes (chimpanzee)	VIL1	XP_001157250.2
Danio rerio (zebrafish)	vil1	NP_956532.1
Oryza sativa (rice)	Os08g0240800	NP_001061335.1
Oryza sativa (rice)	Os06g0659300	NP_001058263.1
Canis lupus familiaris (dog)	VIL1	XP_545642.2
Rattus norvegicus (Norway rat)	Vil1	NP_001101694.1
Oryza sativa (rice)	Os04g0604000	NP_001053784.1

Gene Ontology
Bibliography

Related articles in PubMed

The unusual internal motion of the villin headpiece subdomain.
Harpole KW, O'Brien ES, Clark MA, McKnight CJ, Vugmeyster L, Wand AJ
Protein science : a publication of the Protein Society25(2)423-32(2016 Feb)

A compact native 24-residue supersecondary structure derived from the villin headpiece subdomain.
Hocking HG, H?se F, Madl T, Zacharias M, Rief M, ?old?k G
Biophysical journal108(3)678-86(2015 Feb)

Slow motions in the hydrophobic core of chicken villin headpiece subdomain and their contributions to configurational entropy and heat capacity from solid-state deuteron NMR measurements.
Vugmeyster L, Ostrovsky D, Khadjinova A, Ellden J, Hoatson GL, Vold RL
Biochemistry50(49)10637-46(2011 Dec)

Competition between intradomain and interdomain interactions: a buried salt bridge is essential for villin headpiece folding and actin binding.
Packer LE, Song B, Raleigh DP, McKnight CJ
Biochemistry50(18)3706-12(2011 May)

Heterogeneity and dynamics in villin headpiece crystal structures.
Meng J, McKnight CJ
Acta crystallographica. Section D, Biological crystallography65(Pt 5)470-6(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The thermostable 36-residue subdomain of the villin headpiece (HP36) is the smallest known cooperatively folding protein. The side chain methyl dynamics of villin HP36 has been measured across a wide range of temperatures.
Title: The unusual internal motion of the villin headpiece subdomain.

A compact native 24-residues-long supersecondary structure derived from the hyperstable villin headpiece subdomain consisting of helices 2 and 3, is described.
Title: A compact native 24-residue supersecondary structure derived from the villin headpiece subdomain.

side-chain dynamics of several key hydrophobic methyl groups in chicken villin headpiece subdomain (HP36)
Title: Slow motions in the hydrophobic core of chicken villin headpiece subdomain and their contributions to configurational entropy and heat capacity from solid-state deuteron NMR measurements.

a buried salt bridge is essential for villin headpiece folding and actin binding
Title: Competition between intradomain and interdomain interactions: a buried salt bridge is essential for villin headpiece folding and actin binding.

Villin headpiece domain HP67 & its mutant (H41Y) are resolved in different crystal form & space groups. 2 molecules occur in each asymmetric unit in the new space group P6(1) differing in harmonic motions.
Title: Heterogeneity and dynamics in villin headpiece crystal structures.

The following VIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa27899	NM_205442.1 Latest version!	Gallus gallus villin 1 (VIL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VIL1 ( NM_205442.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990773.1 Gallus gallus villin 1 (VIL1), mRNA.

Price & Availability↑

CloneID	OGa27899
Clone ID Related Accession (Same CDS sequence)	NM_205442.1
Accession Version	NM_205442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Gallus gallus(chicken)
Product	villin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03781.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03781.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGGAGC TCAGCAAAAA GGTCACCGGG AAGCTGGACA AGACCACGCC GGGCATCCAG 
ATATGGAGAA TCGAGAACAT GGAGATGGTG CCGGTGCCCA CTAAAAGCTA TGGCAACTTC 
TACGAGGGGG ACTGCTACGT GCTGCTGTCG ACACGTAAGA CTGGGAGCGG CTTCAGCTAC 
AACATCCACT ACTGGCTGGG TAAGAACTCG AGCCAGGACG AGCAGGGGGC GGCCGCGATC 
TACACCACGC AGATGGATGA GTATCTGGGC TCTGTGGCCG TGCAGCACCG TGAGGTCCAG 
GGCCATGAGA GCGAGACGTT CCGTGCATAC TTCAAGCAGG GACTCATCTA TAAGCAGGGT 
GGGGTGGCCT CAGGCATGAA GCACGTGGAG ACCAACACCT ACAACGTCCA GCGCCTGCTG 
CATGTGAAGG GCAAGAAGAA CGTGGTGGCT GCAGAGGTGG AGATGAGCTG GAAAAGCTTT 
AACCTGGGCG ATGTCTTCCT GCTGGACCTC GGCCAGCTCA TCATCCAGTG GAACGGCCCC 
GAGAGCAATC GTGCCGAAAG GCTGAGGGCA ATGACCCTGG CCAAGGACAT TCGGGACCGA 
GAGCGTGCGG GACGTGCGAA GGTTGGCGTG GTGGAGGGGG AGAATGAGGC GGCCTCACCT 
GAGCTGATGC AGGCCTTGAC ACATGTGCTG GGGGAGAAGA AGAACATCAA GGCAGCCACA 
CCCGATGAAC AAGTTCACCA GGCGCTCAAC AGTGCCCTCA AGCTCTACCA TGTCTCTGAT 
GCCAGTGGGA ACCTGGTCAT ACAAGAGGTA GCAATTCGCC CCTTGACTCA AGATATGCTC 
CAGCATGAGG ACTGCTACAT CCTTGATCAA GCAGGTCTCA AGATCTTTGT GTGGAAGGGC 
AAGAATGCCA ACAAGGAGGA GAAGCAGCAG GCAATGAGCA GGGCCCTGGG CTTCATCAAA 
GCCAAGAACT ACCTGGCCAG CACCAGCGTG GAGACAGAGA ACGATGGGTC TGAGTCCGCT 
GTCTTCAGGC AGCTCTTCCA AAAATGGACT GTTCCCAACC AAACCAGTGG GCTGGGCAAG 
ACCCATACTG TGGGCAAAGT GGCTAAGGTG GAACAGGTGA AGTTTGACGC CACCACAATG 
CATGTCAAGC CTGAAGTGGC CGCCCAGCAG AAGATGGTGG ATGATGGATC TGGGGAGGCA 
GAGGTCTGGC GGGTGGAGAA CCAGGAGCTG GTGCCCGTGG AGAAGCGGTG GTTGGGCCAT 
TTCTACGGCG GGGACTGCTA CCTGGTGCTC TACACCTACT ACGTGGGGCC CAAGGTGAAC 
CGCATCATCT ACATCTGGCA GGGCCGCCAT GCCAGCACGG ATGAGCTGGC CGCCTCGGCC 
TACCAAGCCG TCTTCCTGGA CCAGAAGTAC AACAACGAGC CTGTGCAGGT GCGCGTCACC 
ATGGGCAAGG AGCCGGCCCA CCTGATGGCG ATCTTCAAGG GCAAGATGGT GGTGTACGAG 
AACGGCTCCT CGCGGGCGGG CGGCACGGAG CCGGCGTCCT CCACTCGGCT CTTCCATGTG 
CACGGCACCA ACGAGTACAA CACCAAGGCC TTCGAGGTGC CCGTCCGAGC CGCTTCTCTC 
AACTCCAACG ATGTCTTTGT GCTCAAGACG CCCAGCTCCT GCTACCTCTG GTATGGGAAG 
GGCTGCAGCG GGGATGAGCG TGAGATGGGC AAGATGGTGG CCGACATCAT CTCCAAGACG 
GAGAAGCCGG TGGTCGCTGA AGGGCAGGAG CCGCCCGAGT TCTGGGTAGC TCTGGGCGGC 
AAGACCAGCT ACGCCAACAG CAAGAGGCTA CAGGAAGAGA ATCCCTCTGT GCCCCCCCGG 
CTCTTTGAAT GCTCTAACAA GACAGGCAGG TTCTTGGCCA CTGAGATCGT CGACTTCACC 
CAGGATGACC TGGACGAGAA CGACGTTTAC CTGCTGGACA CTTGGGACCA GATTTTCTTC 
TGGATCGGGA AAGGTGCCAA TGAGTCAGAG AAGGAGGCGG CAGCAGAGAC AGCACAGGAG 
TACCTGCGGA GTCACCCCGG CAGCCGTGAC CTCGACACCC CCATCATCGT GGTGAAGCAG 
GGCTTTGAAC CCCCCACCTT CACTGGCTGG TTCATGGCCT GGGATCCCCT CTGCTGGAGC 
GACAGGAAAT CCTACGATGA GCTGAAAGCT GAGCTGGGGG ACAATGCCAG CATTGGGCAA 
CTTGTGTCAG GGCTCACCTC CAAGAATGAG GTCTTCACGG CCACCACCAC ACTTGTCCCC 
ACCAAGCTGG AGACCTTCCC ACTGGACGTG CTGGTGAACA CTGCAGCTGA GGACCTGCCC 
CGGGGTGTGG ATCCCAGCAG GAAGGAGAAC CACCTCTCTG ACGAGGACTT CAAGGCTGTT 
TTTGGCATGA CCCGCTCTGC CTTTGCCAAC TTGCCCTTGT GGAAACAGCA GAACCTCAAG 
AAGGAGAAAG GACTCTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990773.1
CDS	31..2511
Translation	MVELSKKVTGKLDKTTPGIQIWRIENMEMVPVPTKSYGNFYEGDCYVLLSTRKTGSGFSYNIHYWLGKNSSQDEQGAAAIYTTQMDEYLGSVAVQHREVQGHESETFRAYFKQGLIYKQGGVASGMKHVETNTYNVQRLLHVKGKKNVVAAEVEMSWKSFNLGDVFLLDLGQLIIQWNGPESNRAERLRAMTLAKDIRDRERAGRAKVGVVEGENEAASPELMQALTHVLGEKKNIKAATPDEQVHQALNSALKLYHVSDASGNLVIQEVAIRPLTQDMLQHEDCYILDQAGLKIFVWKGKNANKEEKQQAMSRALGFIKAKNYLASTSVETENDGSESAVFRQLFQKWTVPNQTSGLGKTHTVGKVAKVEQVKFDATTMHVKPEVAAQQKMVDDGSGEAEVWRVENQELVPVEKRWLGHFYGGDCYLVLYTYYVGPKVNRIIYIWQGRHASTDELAASAYQAVFLDQKYNNEPVQVRVTMGKEPAHLMAIFKGKMVVYENGSSRAGGTEPASSTRLFHVHGTNEYNTKAFEVPVRAASLNSNDVFVLKTPSSCYLWYGKGCSGDEREMGKMVADIISKTEKPVVAEGQEPPEFWVALGGKTSYANSKRLQEENPSVPPRLFECSNKTGRFLATEIVDFTQDDLDENDVYLLDTWDQIFFWIGKGANESEKEAAAETAQEYLRSHPGSRDLDTPIIVVKQGFEPPTFTGWFMAWDPLCWSDRKSYDELKAELGDNASIGQLVSGLTSKNEVFTATTTLVPTKLETFPLDVLVNTAAEDLPRGVDPSRKENHLSDEDFKAVFGMTRSAFANLPLWKQQNLKKEKGLF

Target ORF information:

RefSeq Version	NM_205442.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus villin 1 (VIL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205442.1

ORF Insert Sequence:

ATGGTGGAGC TCAGCAAAAA GGTCACCGGG AAGCTGGACA AGACCACGCC GGGCATCCAG 
ATATGGAGAA TCGAGAACAT GGAGATGGTG CCGGTGCCCA CTAAAAGCTA TGGCAACTTC 
TACGAGGGGG ACTGCTACGT GCTGCTGTCG ACACGTAAGA CTGGGAGCGG CTTCAGCTAC 
AACATCCACT ACTGGCTGGG TAAGAACTCG AGCCAGGACG AGCAGGGGGC GGCCGCGATC 
TACACCACGC AGATGGATGA GTATCTGGGC TCTGTGGCCG TGCAGCACCG TGAGGTCCAG 
GGCCATGAGA GCGAGACGTT CCGTGCATAC TTCAAGCAGG GACTCATCTA TAAGCAGGGT 
GGGGTGGCCT CAGGCATGAA GCACGTGGAG ACCAACACCT ACAACGTCCA GCGCCTGCTG 
CATGTGAAGG GCAAGAAGAA CGTGGTGGCT GCAGAGGTGG AGATGAGCTG GAAAAGCTTT 
AACCTGGGCG ATGTCTTCCT GCTGGACCTC GGCCAGCTCA TCATCCAGTG GAACGGCCCC 
GAGAGCAATC GTGCCGAAAG GCTGAGGGCA ATGACCCTGG CCAAGGACAT TCGGGACCGA 
GAGCGTGCGG GACGTGCGAA GGTTGGCGTG GTGGAGGGGG AGAATGAGGC GGCCTCACCT 
GAGCTGATGC AGGCCTTGAC ACATGTGCTG GGGGAGAAGA AGAACATCAA GGCAGCCACA 
CCCGATGAAC AAGTTCACCA GGCGCTCAAC AGTGCCCTCA AGCTCTACCA TGTCTCTGAT 
GCCAGTGGGA ACCTGGTCAT ACAAGAGGTA GCAATTCGCC CCTTGACTCA AGATATGCTC 
CAGCATGAGG ACTGCTACAT CCTTGATCAA GCAGGTCTCA AGATCTTTGT GTGGAAGGGC 
AAGAATGCCA ACAAGGAGGA GAAGCAGCAG GCAATGAGCA GGGCCCTGGG CTTCATCAAA 
GCCAAGAACT ACCTGGCCAG CACCAGCGTG GAGACAGAGA ACGATGGGTC TGAGTCCGCT 
GTCTTCAGGC AGCTCTTCCA AAAATGGACT GTTCCCAACC AAACCAGTGG GCTGGGCAAG 
ACCCATACTG TGGGCAAAGT GGCTAAGGTG GAACAGGTGA AGTTTGACGC CACCACAATG 
CATGTCAAGC CTGAAGTGGC CGCCCAGCAG AAGATGGTGG ATGATGGATC TGGGGAGGCA 
GAGGTCTGGC GGGTGGAGAA CCAGGAGCTG GTGCCCGTGG AGAAGCGGTG GTTGGGCCAT 
TTCTACGGCG GGGACTGCTA CCTGGTGCTC TACACCTACT ACGTGGGGCC CAAGGTGAAC 
CGCATCATCT ACATCTGGCA GGGCCGCCAT GCCAGCACGG ATGAGCTGGC CGCCTCGGCC 
TACCAAGCCG TCTTCCTGGA CCAGAAGTAC AACAACGAGC CTGTGCAGGT GCGCGTCACC 
ATGGGCAAGG AGCCGGCCCA CCTGATGGCG ATCTTCAAGG GCAAGATGGT GGTGTACGAG 
AACGGCTCCT CGCGGGCGGG CGGCACGGAG CCGGCGTCCT CCACTCGGCT CTTCCATGTG 
CACGGCACCA ACGAGTACAA CACCAAGGCC TTCGAGGTGC CCGTCCGAGC CGCTTCTCTC 
AACTCCAACG ATGTCTTTGT GCTCAAGACG CCCAGCTCCT GCTACCTCTG GTATGGGAAG 
GGCTGCAGCG GGGATGAGCG TGAGATGGGC AAGATGGTGG CCGACATCAT CTCCAAGACG 
GAGAAGCCGG TGGTCGCTGA AGGGCAGGAG CCGCCCGAGT TCTGGGTAGC TCTGGGCGGC 
AAGACCAGCT ACGCCAACAG CAAGAGGCTA CAGGAAGAGA ATCCCTCTGT GCCCCCCCGG 
CTCTTTGAAT GCTCTAACAA GACAGGCAGG TTCTTGGCCA CTGAGATCGT CGACTTCACC 
CAGGATGACC TGGACGAGAA CGACGTTTAC CTGCTGGACA CTTGGGACCA GATTTTCTTC 
TGGATCGGGA AAGGTGCCAA TGAGTCAGAG AAGGAGGCGG CAGCAGAGAC AGCACAGGAG 
TACCTGCGGA GTCACCCCGG CAGCCGTGAC CTCGACACCC CCATCATCGT GGTGAAGCAG 
GGCTTTGAAC CCCCCACCTT CACTGGCTGG TTCATGGCCT GGGATCCCCT CTGCTGGAGC 
GACAGGAAAT CCTACGATGA GCTGAAAGCT GAGCTGGGGG ACAATGCCAG CATTGGGCAA 
CTTGTGTCAG GGCTCACCTC CAAGAATGAG GTCTTCACGG CCACCACCAC ACTTGTCCCC 
ACCAAGCTGG AGACCTTCCC ACTGGACGTG CTGGTGAACA CTGCAGCTGA GGACCTGCCC 
CGGGGTGTGG ATCCCAGCAG GAAGGAGAAC CACCTCTCTG ACGAGGACTT CAAGGCTGTT 
TTTGGCATGA CCCGCTCTGC CTTTGCCAAC TTGCCCTTGT GGAAACAGCA GAACCTCAAG 
AAGGAGAAAG GACTCTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The unusual internal motion of the villin headpiece subdomain.
Protein science : a publication of the Protein Society25(2)423-32(2016 Feb)
Harpole KW,O'Brien ES,Clark MA,McKnight CJ,Vugmeyster L,Wand AJ

A compact native 24-residue supersecondary structure derived from the villin headpiece subdomain.
Biophysical journal108(3)678-86(2015 Feb)
Hocking HG,H?se F,Madl T,Zacharias M,Rief M,?old?k G

Slow motions in the hydrophobic core of chicken villin headpiece subdomain and their contributions to configurational entropy and heat capacity from solid-state deuteron NMR measurements.
Biochemistry50(49)10637-46(2011 Dec)
Vugmeyster L,Ostrovsky D,Khadjinova A,Ellden J,Hoatson GL,Vold RL

Competition between intradomain and interdomain interactions: a buried salt bridge is essential for villin headpiece folding and actin binding.
Biochemistry50(18)3706-12(2011 May)
Packer LE,Song B,Raleigh DP,McKnight CJ

Heterogeneity and dynamics in villin headpiece crystal structures.
Acta crystallographica. Section D, Biological crystallography65(Pt 5)470-6(2009 May)
Meng J,McKnight CJ

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VIL1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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