AT5G45650 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT5G45650
Entrez Gene ID	834605
Full Name	subtilase family protein
Synonyms	MRA19.5,MRA19_5
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001344636.1	NP_001318744.1	subtilase family protein
NM_123933.3	NP_199378.1	subtilase family protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT5G45650	NP_199378.1
Arabidopsis thaliana (thale cress)	AT5G45640	NP_199377.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT5G45650 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT5G45650 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb20650	NM_001344636.1 Latest version!	Arabidopsis thaliana subtilase family protein (AT5G45650), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb20650	NM_123933.3 Latest version!	Arabidopsis thaliana subtilase family protein (AT5G45650), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT5G45650 ( NM_001344636.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001318744.1 Arabidopsis thaliana subtilase family protein (AT5G45650), mRNA.

Price & Availability↑

CloneID	OAb20650
Clone ID Related Accession (Same CDS sequence)	NM_001344636.1 , NM_123933.3
Accession Version	NM_001344636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	subtilase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGAAGAAGC TTACTTCTCT CTTCCCTCTC CTCTTTCTCA TTCCCCTCTT AGCCTCATGT 
GCTGAAGAAA AACAGGTGTA TATTGTCTAT TTTGGAGAAC ATAAAGGTGA TAAAGCCTTT 
CATGAAATTG AAGAACATCA TCATTCTTAT CTTCAATCGG TTAAAGAATC CGAAGAAGAC 
GCTAGAGCAT CGCTCTTGTA TAGCTACAAA CATAGCATCA ATGGCTTCGC GGCTGAGCTA 
ACCCCTGACC AAGCCTCTAA ACTAGAAAAA TTAGCAGAGG TTGTTTCAGT ATTCAAGAGC 
CATCCAAGAA AATATGAAGC ACATACGACA CGGTCATGGG AATTTGTAGG ATTGGAGGAG 
GAAGAAACCG ACAGTGATGT TCCACGTCGA AAAAATGATG CCGATGATCG GTTTCGTGTG 
GGAAGAAATT TTTTGAAGAA GGCAAAGCAT GGTGATGGAA TCATCGTCGG TGTTCTCGAC 
AGTGGTGTGT GGCCAGAATC AAAGAGTTTC AATGACAAAG GAATGGGACC AGTTCCAAAA 
TCATGGAAAG GAATCTGCCA AACCGGAGTT GCCTTTAACT CTTCTCACTG TAACCGGAAA 
ATTATTGGAG CGAGGTATTA CGTGAAAGGG TACGAAAGAT ATTACGGAGC GTTCAACGCT 
ACAGCAAACA AAGACTTCTT GTCTCCAAGA GATCCCGACG GACATGGGTC CCACACAGCT 
TCTACTGCCG TTGGCCGTCG AGTTCTCGGT GCATCCGCAC TTGGCGGTTT CGCCAAGGGC 
TCGGCATCGG GCGGTGCACC GTTAGCTCGT CTAGCTATTT ACAAAGCTTG TTGGGCCAAG 
CCCAATGCGG AAAAAGTTGA AGGAAATATC TGTCTAGAAG AGGACATGCT TGCCGCGATC 
GATGACGCCA TCGCTGATGG CGTTCACGTC ATCAGCATTT CCATCGGAAC GACCGAGCCT 
TTTCCATTTA CACAAGATGG AATCGCGATG GGGGCGTTGC ATGCGGTTAA GAGGAATATT 
GTGGTTGCTG CTAGCGCTGG GAATTCAGGG CCAAAGCCGG GAACTTTGTC GAATCTGGCA 
CCGTGGATTA TAACTGTCGG AGCTAGTACT CTTGATCGGG CTTTCGTTGG CGGTCTTGTT 
CTTGGCAATG GCTATACAAT CAAGACGGAT TCGATAACGG CATTTAAAAT GGACAAATTT 
GCCCCTCTTG TTTACGCTTC TAACGTGGTT GTTCCCGGCA TTGCATTGAA CGAGACATCG 
CAATGTTTAC CAAATTCACT AAAACCGGAG CTTGTGAGTG GTAAAGTGGT CTTGTGTTTA 
AGAGGAGCCG GTTCAAGAAT CGGTAAAGGC ATGGAGGTAA AACGAGCTGG AGGAGCCGGT 
ATGATTCTCG GAAATATCGC AGCTAACGGC AATGAAGTTC CTTCAGACTC TCATTTTGTT 
CCGACTGCCG GGGTTACCCC AACCGTGGTC GATAAAATTC TGGAGTACAT CAAAACCGAT 
AAAAACCCGA AGGCTTTTAT CAAACCGGGG AAAACGGTTT ACAAATACCA AGCAGCTCCT 
TCCATGACCG GGTTTTCTAG CCGCGGCCCA AACGTTGTAG ACCCCAACAT TCTAAAGCCG 
GATATAACCG CCCCAGGACT GTACATACTG GCTGCATGGA GCGGAGCAGA TTCACCGAGC 
AAAATGTCAG TAGATCAAAG AGTTGCAGGT TATAACATCT ACTCAGGAAC TTCAATGTCT 
TGTCCTCATG TCGCTGGTGC AATTGCTCTT CTCAAAGCCA TTCATCCCAA ATGGAGTAGC 
GCTGCTATAA GATCAGCTCT TATGACCACT GCTTGGATGA CTAACGACAA GAAGAAACCG 
ATCCAAGACA CTACTGGTTT ACCAGCAAAC CCATTTGCAC TTGGGTCAGG ACATTTCAGA 
CCAACAAAAG CTGCAGATCC CGGTTTAGTC TATGATGCAT CATACCGAGC TTACCTTCTC 
TATGGCTGCT CGGTTAACAT CACCAATATT GACCCGACAT TCAAATGTCC TAGTAAAATT 
CCGCCCGGTT ATAATCACAA TTACCCGTCA ATCGCGGTCC CGAATCTCAA GAAGACGGTG 
ACCGTTAAAA GAACAGTTAC AAATGTTGGA ACTGGTAACT CAACAAGCAC ATACTTATTC 
AGCGTTAAAC CGCCATCGGG TATTTCGGTT AAGGCTATAC CGAATATATT GTCCTTTAAC 
CGGATTGGTC AGAAGCAGCG GTTTAAAATC GTTATTAAAC CACTGAAGAA CCAGGTGATG 
AATGCGACTG AAAAGGGTCA ATACCAATTT GGATGGTTTA GTTGGACAGA TAAAGTCCAT 
GTTGTGAGGA GTCCAATTGC TGTTTCCTTA GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318744.1
CDS	85..2460
Translation	MKKLTSLFPLLFLIPLLASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSITAFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKVHVVRSPIAVSLA

Target ORF information:

RefSeq Version	NM_001344636.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana subtilase family protein (AT5G45650), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344636.1

ORF Insert Sequence:

ATGAAGAAGC TTACTTCTCT CTTCCCTCTC CTCTTTCTCA TTCCCCTCTT AGCCTCATGT 
GCTGAAGAAA AACAGGTGTA TATTGTCTAT TTTGGAGAAC ATAAAGGTGA TAAAGCCTTT 
CATGAAATTG AAGAACATCA TCATTCTTAT CTTCAATCGG TTAAAGAATC CGAAGAAGAC 
GCTAGAGCAT CGCTCTTGTA TAGCTACAAA CATAGCATCA ATGGCTTCGC GGCTGAGCTA 
ACCCCTGACC AAGCCTCTAA ACTAGAAAAA TTAGCAGAGG TTGTTTCAGT ATTCAAGAGC 
CATCCAAGAA AATATGAAGC ACATACGACA CGGTCATGGG AATTTGTAGG ATTGGAGGAG 
GAAGAAACCG ACAGTGATGT TCCACGTCGA AAAAATGATG CCGATGATCG GTTTCGTGTG 
GGAAGAAATT TTTTGAAGAA GGCAAAGCAT GGTGATGGAA TCATCGTCGG TGTTCTCGAC 
AGTGGTGTGT GGCCAGAATC AAAGAGTTTC AATGACAAAG GAATGGGACC AGTTCCAAAA 
TCATGGAAAG GAATCTGCCA AACCGGAGTT GCCTTTAACT CTTCTCACTG TAACCGGAAA 
ATTATTGGAG CGAGGTATTA CGTGAAAGGG TACGAAAGAT ATTACGGAGC GTTCAACGCT 
ACAGCAAACA AAGACTTCTT GTCTCCAAGA GATCCCGACG GACATGGGTC CCACACAGCT 
TCTACTGCCG TTGGCCGTCG AGTTCTCGGT GCATCCGCAC TTGGCGGTTT CGCCAAGGGC 
TCGGCATCGG GCGGTGCACC GTTAGCTCGT CTAGCTATTT ACAAAGCTTG TTGGGCCAAG 
CCCAATGCGG AAAAAGTTGA AGGAAATATC TGTCTAGAAG AGGACATGCT TGCCGCGATC 
GATGACGCCA TCGCTGATGG CGTTCACGTC ATCAGCATTT CCATCGGAAC GACCGAGCCT 
TTTCCATTTA CACAAGATGG AATCGCGATG GGGGCGTTGC ATGCGGTTAA GAGGAATATT 
GTGGTTGCTG CTAGCGCTGG GAATTCAGGG CCAAAGCCGG GAACTTTGTC GAATCTGGCA 
CCGTGGATTA TAACTGTCGG AGCTAGTACT CTTGATCGGG CTTTCGTTGG CGGTCTTGTT 
CTTGGCAATG GCTATACAAT CAAGACGGAT TCGATAACGG CATTTAAAAT GGACAAATTT 
GCCCCTCTTG TTTACGCTTC TAACGTGGTT GTTCCCGGCA TTGCATTGAA CGAGACATCG 
CAATGTTTAC CAAATTCACT AAAACCGGAG CTTGTGAGTG GTAAAGTGGT CTTGTGTTTA 
AGAGGAGCCG GTTCAAGAAT CGGTAAAGGC ATGGAGGTAA AACGAGCTGG AGGAGCCGGT 
ATGATTCTCG GAAATATCGC AGCTAACGGC AATGAAGTTC CTTCAGACTC TCATTTTGTT 
CCGACTGCCG GGGTTACCCC AACCGTGGTC GATAAAATTC TGGAGTACAT CAAAACCGAT 
AAAAACCCGA AGGCTTTTAT CAAACCGGGG AAAACGGTTT ACAAATACCA AGCAGCTCCT 
TCCATGACCG GGTTTTCTAG CCGCGGCCCA AACGTTGTAG ACCCCAACAT TCTAAAGCCG 
GATATAACCG CCCCAGGACT GTACATACTG GCTGCATGGA GCGGAGCAGA TTCACCGAGC 
AAAATGTCAG TAGATCAAAG AGTTGCAGGT TATAACATCT ACTCAGGAAC TTCAATGTCT 
TGTCCTCATG TCGCTGGTGC AATTGCTCTT CTCAAAGCCA TTCATCCCAA ATGGAGTAGC 
GCTGCTATAA GATCAGCTCT TATGACCACT GCTTGGATGA CTAACGACAA GAAGAAACCG 
ATCCAAGACA CTACTGGTTT ACCAGCAAAC CCATTTGCAC TTGGGTCAGG ACATTTCAGA 
CCAACAAAAG CTGCAGATCC CGGTTTAGTC TATGATGCAT CATACCGAGC TTACCTTCTC 
TATGGCTGCT CGGTTAACAT CACCAATATT GACCCGACAT TCAAATGTCC TAGTAAAATT 
CCGCCCGGTT ATAATCACAA TTACCCGTCA ATCGCGGTCC CGAATCTCAA GAAGACGGTG 
ACCGTTAAAA GAACAGTTAC AAATGTTGGA ACTGGTAACT CAACAAGCAC ATACTTATTC 
AGCGTTAAAC CGCCATCGGG TATTTCGGTT AAGGCTATAC CGAATATATT GTCCTTTAAC 
CGGATTGGTC AGAAGCAGCG GTTTAAAATC GTTATTAAAC CACTGAAGAA CCAGGTGATG 
AATGCGACTG AAAAGGGTCA ATACCAATTT GGATGGTTTA GTTGGACAGA TAAAGTCCAT 
GTTGTGAGGA GTCCAATTGC TGTTTCCTTA GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT5G45650 ( NM_123933.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199378.1 Arabidopsis thaliana subtilase family protein (AT5G45650), mRNA.

Price & Availability↑

CloneID	OAb20650
Clone ID Related Accession (Same CDS sequence)	NM_001344636.1 , NM_123933.3
Accession Version	NM_123933.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	subtilase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123933.2.

ATGAAGAAGC TTACTTCTCT CTTCCCTCTC CTCTTTCTCA TTCCCCTCTT AGCCTCATGT 
GCTGAAGAAA AACAGGTGTA TATTGTCTAT TTTGGAGAAC ATAAAGGTGA TAAAGCCTTT 
CATGAAATTG AAGAACATCA TCATTCTTAT CTTCAATCGG TTAAAGAATC CGAAGAAGAC 
GCTAGAGCAT CGCTCTTGTA TAGCTACAAA CATAGCATCA ATGGCTTCGC GGCTGAGCTA 
ACCCCTGACC AAGCCTCTAA ACTAGAAAAA TTAGCAGAGG TTGTTTCAGT ATTCAAGAGC 
CATCCAAGAA AATATGAAGC ACATACGACA CGGTCATGGG AATTTGTAGG ATTGGAGGAG 
GAAGAAACCG ACAGTGATGT TCCACGTCGA AAAAATGATG CCGATGATCG GTTTCGTGTG 
GGAAGAAATT TTTTGAAGAA GGCAAAGCAT GGTGATGGAA TCATCGTCGG TGTTCTCGAC 
AGTGGTGTGT GGCCAGAATC AAAGAGTTTC AATGACAAAG GAATGGGACC AGTTCCAAAA 
TCATGGAAAG GAATCTGCCA AACCGGAGTT GCCTTTAACT CTTCTCACTG TAACCGGAAA 
ATTATTGGAG CGAGGTATTA CGTGAAAGGG TACGAAAGAT ATTACGGAGC GTTCAACGCT 
ACAGCAAACA AAGACTTCTT GTCTCCAAGA GATCCCGACG GACATGGGTC CCACACAGCT 
TCTACTGCCG TTGGCCGTCG AGTTCTCGGT GCATCCGCAC TTGGCGGTTT CGCCAAGGGC 
TCGGCATCGG GCGGTGCACC GTTAGCTCGT CTAGCTATTT ACAAAGCTTG TTGGGCCAAG 
CCCAATGCGG AAAAAGTTGA AGGAAATATC TGTCTAGAAG AGGACATGCT TGCCGCGATC 
GATGACGCCA TCGCTGATGG CGTTCACGTC ATCAGCATTT CCATCGGAAC GACCGAGCCT 
TTTCCATTTA CACAAGATGG AATCGCGATG GGGGCGTTGC ATGCGGTTAA GAGGAATATT 
GTGGTTGCTG CTAGCGCTGG GAATTCAGGG CCAAAGCCGG GAACTTTGTC GAATCTGGCA 
CCGTGGATTA TAACTGTCGG AGCTAGTACT CTTGATCGGG CTTTCGTTGG CGGTCTTGTT 
CTTGGCAATG GCTATACAAT CAAGACGGAT TCGATAACGG CATTTAAAAT GGACAAATTT 
GCCCCTCTTG TTTACGCTTC TAACGTGGTT GTTCCCGGCA TTGCATTGAA CGAGACATCG 
CAATGTTTAC CAAATTCACT AAAACCGGAG CTTGTGAGTG GTAAAGTGGT CTTGTGTTTA 
AGAGGAGCCG GTTCAAGAAT CGGTAAAGGC ATGGAGGTAA AACGAGCTGG AGGAGCCGGT 
ATGATTCTCG GAAATATCGC AGCTAACGGC AATGAAGTTC CTTCAGACTC TCATTTTGTT 
CCGACTGCCG GGGTTACCCC AACCGTGGTC GATAAAATTC TGGAGTACAT CAAAACCGAT 
AAAAACCCGA AGGCTTTTAT CAAACCGGGG AAAACGGTTT ACAAATACCA AGCAGCTCCT 
TCCATGACCG GGTTTTCTAG CCGCGGCCCA AACGTTGTAG ACCCCAACAT TCTAAAGCCG 
GATATAACCG CCCCAGGACT GTACATACTG GCTGCATGGA GCGGAGCAGA TTCACCGAGC 
AAAATGTCAG TAGATCAAAG AGTTGCAGGT TATAACATCT ACTCAGGAAC TTCAATGTCT 
TGTCCTCATG TCGCTGGTGC AATTGCTCTT CTCAAAGCCA TTCATCCCAA ATGGAGTAGC 
GCTGCTATAA GATCAGCTCT TATGACCACT GCTTGGATGA CTAACGACAA GAAGAAACCG 
ATCCAAGACA CTACTGGTTT ACCAGCAAAC CCATTTGCAC TTGGGTCAGG ACATTTCAGA 
CCAACAAAAG CTGCAGATCC CGGTTTAGTC TATGATGCAT CATACCGAGC TTACCTTCTC 
TATGGCTGCT CGGTTAACAT CACCAATATT GACCCGACAT TCAAATGTCC TAGTAAAATT 
CCGCCCGGTT ATAATCACAA TTACCCGTCA ATCGCGGTCC CGAATCTCAA GAAGACGGTG 
ACCGTTAAAA GAACAGTTAC AAATGTTGGA ACTGGTAACT CAACAAGCAC ATACTTATTC 
AGCGTTAAAC CGCCATCGGG TATTTCGGTT AAGGCTATAC CGAATATATT GTCCTTTAAC 
CGGATTGGTC AGAAGCAGCG GTTTAAAATC GTTATTAAAC CACTGAAGAA CCAGGTGATG 
AATGCGACTG AAAAGGGTCA ATACCAATTT GGATGGTTTA GTTGGACAGA TAAAGTCCAT 
GTTGTGAGGA GTCCAATTGC TGTTTCCTTA GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199378.1
CDS	120..2495
Translation	MKKLTSLFPLLFLIPLLASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSITAFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKVHVVRSPIAVSLA

Target ORF information:

RefSeq Version	NM_123933.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana subtilase family protein (AT5G45650), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123933.3

ORF Insert Sequence:

ATGAAGAAGC TTACTTCTCT CTTCCCTCTC CTCTTTCTCA TTCCCCTCTT AGCCTCATGT 
GCTGAAGAAA AACAGGTGTA TATTGTCTAT TTTGGAGAAC ATAAAGGTGA TAAAGCCTTT 
CATGAAATTG AAGAACATCA TCATTCTTAT CTTCAATCGG TTAAAGAATC CGAAGAAGAC 
GCTAGAGCAT CGCTCTTGTA TAGCTACAAA CATAGCATCA ATGGCTTCGC GGCTGAGCTA 
ACCCCTGACC AAGCCTCTAA ACTAGAAAAA TTAGCAGAGG TTGTTTCAGT ATTCAAGAGC 
CATCCAAGAA AATATGAAGC ACATACGACA CGGTCATGGG AATTTGTAGG ATTGGAGGAG 
GAAGAAACCG ACAGTGATGT TCCACGTCGA AAAAATGATG CCGATGATCG GTTTCGTGTG 
GGAAGAAATT TTTTGAAGAA GGCAAAGCAT GGTGATGGAA TCATCGTCGG TGTTCTCGAC 
AGTGGTGTGT GGCCAGAATC AAAGAGTTTC AATGACAAAG GAATGGGACC AGTTCCAAAA 
TCATGGAAAG GAATCTGCCA AACCGGAGTT GCCTTTAACT CTTCTCACTG TAACCGGAAA 
ATTATTGGAG CGAGGTATTA CGTGAAAGGG TACGAAAGAT ATTACGGAGC GTTCAACGCT 
ACAGCAAACA AAGACTTCTT GTCTCCAAGA GATCCCGACG GACATGGGTC CCACACAGCT 
TCTACTGCCG TTGGCCGTCG AGTTCTCGGT GCATCCGCAC TTGGCGGTTT CGCCAAGGGC 
TCGGCATCGG GCGGTGCACC GTTAGCTCGT CTAGCTATTT ACAAAGCTTG TTGGGCCAAG 
CCCAATGCGG AAAAAGTTGA AGGAAATATC TGTCTAGAAG AGGACATGCT TGCCGCGATC 
GATGACGCCA TCGCTGATGG CGTTCACGTC ATCAGCATTT CCATCGGAAC GACCGAGCCT 
TTTCCATTTA CACAAGATGG AATCGCGATG GGGGCGTTGC ATGCGGTTAA GAGGAATATT 
GTGGTTGCTG CTAGCGCTGG GAATTCAGGG CCAAAGCCGG GAACTTTGTC GAATCTGGCA 
CCGTGGATTA TAACTGTCGG AGCTAGTACT CTTGATCGGG CTTTCGTTGG CGGTCTTGTT 
CTTGGCAATG GCTATACAAT CAAGACGGAT TCGATAACGG CATTTAAAAT GGACAAATTT 
GCCCCTCTTG TTTACGCTTC TAACGTGGTT GTTCCCGGCA TTGCATTGAA CGAGACATCG 
CAATGTTTAC CAAATTCACT AAAACCGGAG CTTGTGAGTG GTAAAGTGGT CTTGTGTTTA 
AGAGGAGCCG GTTCAAGAAT CGGTAAAGGC ATGGAGGTAA AACGAGCTGG AGGAGCCGGT 
ATGATTCTCG GAAATATCGC AGCTAACGGC AATGAAGTTC CTTCAGACTC TCATTTTGTT 
CCGACTGCCG GGGTTACCCC AACCGTGGTC GATAAAATTC TGGAGTACAT CAAAACCGAT 
AAAAACCCGA AGGCTTTTAT CAAACCGGGG AAAACGGTTT ACAAATACCA AGCAGCTCCT 
TCCATGACCG GGTTTTCTAG CCGCGGCCCA AACGTTGTAG ACCCCAACAT TCTAAAGCCG 
GATATAACCG CCCCAGGACT GTACATACTG GCTGCATGGA GCGGAGCAGA TTCACCGAGC 
AAAATGTCAG TAGATCAAAG AGTTGCAGGT TATAACATCT ACTCAGGAAC TTCAATGTCT 
TGTCCTCATG TCGCTGGTGC AATTGCTCTT CTCAAAGCCA TTCATCCCAA ATGGAGTAGC 
GCTGCTATAA GATCAGCTCT TATGACCACT GCTTGGATGA CTAACGACAA GAAGAAACCG 
ATCCAAGACA CTACTGGTTT ACCAGCAAAC CCATTTGCAC TTGGGTCAGG ACATTTCAGA 
CCAACAAAAG CTGCAGATCC CGGTTTAGTC TATGATGCAT CATACCGAGC TTACCTTCTC 
TATGGCTGCT CGGTTAACAT CACCAATATT GACCCGACAT TCAAATGTCC TAGTAAAATT 
CCGCCCGGTT ATAATCACAA TTACCCGTCA ATCGCGGTCC CGAATCTCAA GAAGACGGTG 
ACCGTTAAAA GAACAGTTAC AAATGTTGGA ACTGGTAACT CAACAAGCAC ATACTTATTC 
AGCGTTAAAC CGCCATCGGG TATTTCGGTT AAGGCTATAC CGAATATATT GTCCTTTAAC 
CGGATTGGTC AGAAGCAGCG GTTTAAAATC GTTATTAAAC CACTGAAGAA CCAGGTGATG 
AATGCGACTG AAAAGGGTCA ATACCAATTT GGATGGTTTA GTTGGACAGA TAAAGTCCAT 
GTTGTGAGGA GTCCAATTGC TGTTTCCTTA GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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AT5G45650 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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