MIRO1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIRO1
Entrez Gene ID	832814
Full Name	MIRO-related GTP-ase 1
Synonyms	F21A20.250,F21A20_250,MIRO-related GTP-ase 1,emb2473,embryo defective 2473
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001036876.2	NP_001031953.1	MIRO-related GTP-ase 1
NM_122636.4	NP_198106.1	MIRO-related GTP-ase 1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADR402W	NP_984497.1
Neurospora crassa	NCU03966	XP_957605.1
Oryza sativa (rice)	Os01g0843300	NP_001044775.1
Pan troglodytes (chimpanzee)	RHOT2	XP_510715.4
Homo sapiens (human)	RHOT2	NP_620124.1
Mus musculus (house mouse)	Rhot2	NP_666111.1
Danio rerio (zebrafish)	rhot2	NP_001032768.1
Kluyveromyces lactis	KLLA0A03465g	XP_451152.1
Magnaporthe oryzae (rice blast fungus)	MGG_01044	XP_003717898.1
Arabidopsis thaliana (thale cress)	MIRO1	NP_198106.1
Oryza sativa (rice)	Os03g0810600	NP_001051665.1
Saccharomyces cerevisiae (baker's yeast)	GEM1	NP_009351.1
	RHOT2	XP_001118510.2
Rattus norvegicus (Norway rat)	Rhot2	NP_861544.1
Canis lupus familiaris (dog)	RHOT2	XP_005622161.1
Bos taurus (cattle)	RHOT2	NP_847886.3
Gallus gallus (chicken)	RHOT2	NP_001074335.1
Schizosaccharomyces pombe (fission yeast)	SPCC320.04c	NP_587725.1
Xenopus tropicalis (tropical clawed frog)	rhot2	NP_001006725.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MIRO1 and MIRO2 are unequally redundant and that a proportion of the miro1(+/-)/miro2(-/-) plants haploid gametes displays the complete null phenotype of MIRO GTPase function at key developmental stages.
Title: Arabidopsis thaliana MIRO1 and MIRO2 GTPases are unequally redundant in pollen tube growth and fusion of polar nuclei during female gametogenesis.

proper mitochondrial morphology and intracellular distribution are maintained by MIRO1 and are vital for embryonic cell division
Title: MIRO1 influences the morphology and intracellular distribution of mitochondria during embryonic cell division in Arabidopsis.

Findings suggest that mitochondrial morphology is influenced by MIRO1 and plays a vital role during embryogenesis and pollen tube growth.
Title: EMB2473/MIRO1, an Arabidopsis Miro GTPase, is required for embryogenesis and influences mitochondrial morphology in pollen.

The following MIRO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIRO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb19670	NM_001036876.2 Latest version!	Arabidopsis thaliana MIRO-related GTP-ase 1 (MIRO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb19670	NM_122636.4 Latest version!	Arabidopsis thaliana MIRO-related GTP-ase 1 (MIRO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIRO1 ( NM_001036876.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001031953.1 Arabidopsis thaliana MIRO-related GTP-ase 1 (MIRO1), mRNA.

Price & Availability↑

CloneID	OAb19670
Clone ID Related Accession (Same CDS sequence)	NM_001036876.2 , NM_122636.4
Accession Version	NM_001036876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	MIRO-related GTP-ase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001036876.1.

ATGGCGAGAT ACGCTGCTGG TGCGGTTGAT TGTCCTGGTT CTCCCAAATC TGTGCGAATC 
GTTGTGGTTG GTGATAAGGG CACTGGAAAA TCAAGCTTGA TTGTTGCTGC AGCCACTGAT 
TCTTTCCCTC CCAATGTCCC TCCTGTTTTG CCTGATTACA AGTTGCCTAT TGAATTCTTC 
CCTGATGGTA TACCCGTCAC CATTGTCGAC ACTTCCTCGA GGCCAGAAGA TAGGGACATT 
GTTGCTGAGG AATTGAAGCG CGCAGATGCA GTAGTTTTGA CATATGCCTG TGATCGACCT 
GAGACTCTCG AACGTTTGAG TGAATACTGG CTTCCAGAGC TTCGGCGATT AGAGGTAAAA 
ATTCCTATTA TCGTAGCAGG ATGTAAGCTT GACTTCAGAG ACGACAATAA CCAGGTCAGC 
CTCGAACAAG TGATGTCACC TATAATGCAG CAGTTTCGGG AGATAGAGAC TTGTATCGAG 
TGTTCTGCCC TGAAGCAACT CCAGGCACAA GAAGTTTTCT ATTATGCACA AAAGACTGTC 
CTTCACCCAA CAGGACCTCT GTTTGATCAA GATTCCCAGG CACTGAAACC CCGGTGTGTA 
AGGGCATTGA AGCGTATCTT TATACTCTGT GATCATGACA GAGATGGTGC TCTTAGCGAG 
GCTGAGTTAA ATGATTTTCA GGTCAAGTGT TTCCATGCAC CATTGCAGCC TTCTGAAATT 
GAAGGTGTTA AGAGGGTTGT GCAAGAAAAG TTGCCAGAAG GTGTGAATGA AAGAGGACTG 
ACAGTCACTG GCTTCCTATT TTTACATGCA CTTTTTATTG AGAAAGGGAG GCTTGAGACT 
ACGTGGACTG TACTCAGGAA GTTTGGGTAT AATAATGATA TAAGACTTGC TGAAGAATTG 
CTTCCATCTG CAATATTCAA GCGAGCTCCT GATCAGAGCT TTGAGTTGAC AAATGCAGCA 
ATCGATTTCC TGAAAGGAAT GTATATGTTG TTTGATGACG ACCAGGATAA CAATCTGAGA 
CCTCAAGAGA TCGAGGATCT TTTTTCAACT GCACCTGAAA GTCCATGGAA GGAAGCTCCT 
TATGAAGATG CTGCGGAAAA AACTGCCCTT GGAGGACTAT CATTTGACGC TTTCCTGTCA 
ATGTGGTCAC TGATGACACT ATTAGAACCG GCTCGGAGTG TGGAGAATTT GATTTACATT 
GGATTCCCGG GTGATCCATC GACTGCCATT CGTGTTACTA GGAGAAGACG TTTAGATCGC 
AAAAAGCAGC AATGTGAACG AAAAGTTTTC CAGTGCTTTG TTTTTGGACC TAATAATGCT 
GGAAAATCTG CATTGCTGAA TTGCTTTCTT GGAAGGTCAT ATACTGATAA CCAGGAATCA 
ACTACTGATG AGCGTTATGC AGTAAATATG GTCGATGAAT CGGGTGCTAA GAAAACTCTT 
ATCATGAGGG AAATTCCAGA AGATGGAGTT CAAGGGCTAT TTTCATCCAA GGAATCCTTA 
GCTGCATGTG ACATAGCTGT CTTCGTGTAT GACAGTTCTG ATGAGTCGTC ATGGAAGAGA 
GCGACCCAGT TGCTTGTAGA AGTTGCAAAT TATGGTGAGG CCACTGGGTA TGAGGTCCCT 
TGCCTCATGG TTTCAGCAAA AGACGATCTT GATTCATCTC CTATTTCCAT ACAAGAATCC 
ACCAGGATGA CACAAGATAT GGGAATAGAG CCTCCGGTAT CAATCAGCTC CAAATTGGGG 
GATTTCAACA ATTTATTCCG CAAAATCTTA ACTGCAGCAC AACATCCTCA TTTGAGCATT 
CCAGAGACCG AAGCAGGCAA AAGCCGCAAA CATTACAACA GGCTAATCAA CCGCTCTCTT 
ATGGCCGTTT CCATTGGTGC TGCTGCTGTG GTCGTTGGGC TGGCTGCATA CCGTGTGTAT 
GCCACAAGAA AGAGCTCGTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001031953.1
CDS	214..2160
Translation	MARYAAGAVDCPGSPKSVRIVVVGDKGTGKSSLIVAAATDSFPPNVPPVLPDYKLPIEFFPDGIPVTIVDTSSRPEDRDIVAEELKRADAVVLTYACDRPETLERLSEYWLPELRRLEVKIPIIVAGCKLDFRDDNNQVSLEQVMSPIMQQFREIETCIECSALKQLQAQEVFYYAQKTVLHPTGPLFDQDSQALKPRCVRALKRIFILCDHDRDGALSEAELNDFQVKCFHAPLQPSEIEGVKRVVQEKLPEGVNERGLTVTGFLFLHALFIEKGRLETTWTVLRKFGYNNDIRLAEELLPSAIFKRAPDQSFELTNAAIDFLKGMYMLFDDDQDNNLRPQEIEDLFSTAPESPWKEAPYEDAAEKTALGGLSFDAFLSMWSLMTLLEPARSVENLIYIGFPGDPSTAIRVTRRRRLDRKKQQCERKVFQCFVFGPNNAGKSALLNCFLGRSYTDNQESTTDERYAVNMVDESGAKKTLIMREIPEDGVQGLFSSKESLAACDIAVFVYDSSDESSWKRATQLLVEVANYGEATGYEVPCLMVSAKDDLDSSPISIQESTRMTQDMGIEPPVSISSKLGDFNNLFRKILTAAQHPHLSIPETEAGKSRKHYNRLINRSLMAVSIGAAAVVVGLAAYRVYATRKSSSA

Target ORF information:

RefSeq Version	NM_001036876.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana MIRO-related GTP-ase 1 (MIRO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001036876.2

ORF Insert Sequence:

ATGGCGAGAT ACGCTGCTGG TGCGGTTGAT TGTCCTGGTT CTCCCAAATC TGTGCGAATC 
GTTGTGGTTG GTGATAAGGG CACTGGAAAA TCAAGCTTGA TTGTTGCTGC AGCCACTGAT 
TCTTTCCCTC CCAATGTCCC TCCTGTTTTG CCTGATTACA AGTTGCCTAT TGAATTCTTC 
CCTGATGGTA TACCCGTCAC CATTGTCGAC ACTTCCTCGA GGCCAGAAGA TAGGGACATT 
GTTGCTGAGG AATTGAAGCG CGCAGATGCA GTAGTTTTGA CATATGCCTG TGATCGACCT 
GAGACTCTCG AACGTTTGAG TGAATACTGG CTTCCAGAGC TTCGGCGATT AGAGGTAAAA 
ATTCCTATTA TCGTAGCAGG ATGTAAGCTT GACTTCAGAG ACGACAATAA CCAGGTCAGC 
CTCGAACAAG TGATGTCACC TATAATGCAG CAGTTTCGGG AGATAGAGAC TTGTATCGAG 
TGTTCTGCCC TGAAGCAACT CCAGGCACAA GAAGTTTTCT ATTATGCACA AAAGACTGTC 
CTTCACCCAA CAGGACCTCT GTTTGATCAA GATTCCCAGG CACTGAAACC CCGGTGTGTA 
AGGGCATTGA AGCGTATCTT TATACTCTGT GATCATGACA GAGATGGTGC TCTTAGCGAG 
GCTGAGTTAA ATGATTTTCA GGTCAAGTGT TTCCATGCAC CATTGCAGCC TTCTGAAATT 
GAAGGTGTTA AGAGGGTTGT GCAAGAAAAG TTGCCAGAAG GTGTGAATGA AAGAGGACTG 
ACAGTCACTG GCTTCCTATT TTTACATGCA CTTTTTATTG AGAAAGGGAG GCTTGAGACT 
ACGTGGACTG TACTCAGGAA GTTTGGGTAT AATAATGATA TAAGACTTGC TGAAGAATTG 
CTTCCATCTG CAATATTCAA GCGAGCTCCT GATCAGAGCT TTGAGTTGAC AAATGCAGCA 
ATCGATTTCC TGAAAGGAAT GTATATGTTG TTTGATGACG ACCAGGATAA CAATCTGAGA 
CCTCAAGAGA TCGAGGATCT TTTTTCAACT GCACCTGAAA GTCCATGGAA GGAAGCTCCT 
TATGAAGATG CTGCGGAAAA AACTGCCCTT GGAGGACTAT CATTTGACGC TTTCCTGTCA 
ATGTGGTCAC TGATGACACT ATTAGAACCG GCTCGGAGTG TGGAGAATTT GATTTACATT 
GGATTCCCGG GTGATCCATC GACTGCCATT CGTGTTACTA GGAGAAGACG TTTAGATCGC 
AAAAAGCAGC AATGTGAACG AAAAGTTTTC CAGTGCTTTG TTTTTGGACC TAATAATGCT 
GGAAAATCTG CATTGCTGAA TTGCTTTCTT GGAAGGTCAT ATACTGATAA CCAGGAATCA 
ACTACTGATG AGCGTTATGC AGTAAATATG GTCGATGAAT CGGGTGCTAA GAAAACTCTT 
ATCATGAGGG AAATTCCAGA AGATGGAGTT CAAGGGCTAT TTTCATCCAA GGAATCCTTA 
GCTGCATGTG ACATAGCTGT CTTCGTGTAT GACAGTTCTG ATGAGTCGTC ATGGAAGAGA 
GCGACCCAGT TGCTTGTAGA AGTTGCAAAT TATGGTGAGG CCACTGGGTA TGAGGTCCCT 
TGCCTCATGG TTTCAGCAAA AGACGATCTT GATTCATCTC CTATTTCCAT ACAAGAATCC 
ACCAGGATGA CACAAGATAT GGGAATAGAG CCTCCGGTAT CAATCAGCTC CAAATTGGGG 
GATTTCAACA ATTTATTCCG CAAAATCTTA ACTGCAGCAC AACATCCTCA TTTGAGCATT 
CCAGAGACCG AAGCAGGCAA AAGCCGCAAA CATTACAACA GGCTAATCAA CCGCTCTCTT 
ATGGCCGTTT CCATTGGTGC TGCTGCTGTG GTCGTTGGGC TGGCTGCATA CCGTGTGTAT 
GCCACAAGAA AGAGCTCGTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIRO1 ( NM_122636.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_198106.1 Arabidopsis thaliana MIRO-related GTP-ase 1 (MIRO1), mRNA.

Price & Availability↑

CloneID	OAb19670
Clone ID Related Accession (Same CDS sequence)	NM_001036876.2 , NM_122636.4
Accession Version	NM_122636.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	MIRO-related GTP-ase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_122636.3.

ATGGCGAGAT ACGCTGCTGG TGCGGTTGAT TGTCCTGGTT CTCCCAAATC TGTGCGAATC 
GTTGTGGTTG GTGATAAGGG CACTGGAAAA TCAAGCTTGA TTGTTGCTGC AGCCACTGAT 
TCTTTCCCTC CCAATGTCCC TCCTGTTTTG CCTGATTACA AGTTGCCTAT TGAATTCTTC 
CCTGATGGTA TACCCGTCAC CATTGTCGAC ACTTCCTCGA GGCCAGAAGA TAGGGACATT 
GTTGCTGAGG AATTGAAGCG CGCAGATGCA GTAGTTTTGA CATATGCCTG TGATCGACCT 
GAGACTCTCG AACGTTTGAG TGAATACTGG CTTCCAGAGC TTCGGCGATT AGAGGTAAAA 
ATTCCTATTA TCGTAGCAGG ATGTAAGCTT GACTTCAGAG ACGACAATAA CCAGGTCAGC 
CTCGAACAAG TGATGTCACC TATAATGCAG CAGTTTCGGG AGATAGAGAC TTGTATCGAG 
TGTTCTGCCC TGAAGCAACT CCAGGCACAA GAAGTTTTCT ATTATGCACA AAAGACTGTC 
CTTCACCCAA CAGGACCTCT GTTTGATCAA GATTCCCAGG CACTGAAACC CCGGTGTGTA 
AGGGCATTGA AGCGTATCTT TATACTCTGT GATCATGACA GAGATGGTGC TCTTAGCGAG 
GCTGAGTTAA ATGATTTTCA GGTCAAGTGT TTCCATGCAC CATTGCAGCC TTCTGAAATT 
GAAGGTGTTA AGAGGGTTGT GCAAGAAAAG TTGCCAGAAG GTGTGAATGA AAGAGGACTG 
ACAGTCACTG GCTTCCTATT TTTACATGCA CTTTTTATTG AGAAAGGGAG GCTTGAGACT 
ACGTGGACTG TACTCAGGAA GTTTGGGTAT AATAATGATA TAAGACTTGC TGAAGAATTG 
CTTCCATCTG CAATATTCAA GCGAGCTCCT GATCAGAGCT TTGAGTTGAC AAATGCAGCA 
ATCGATTTCC TGAAAGGAAT GTATATGTTG TTTGATGACG ACCAGGATAA CAATCTGAGA 
CCTCAAGAGA TCGAGGATCT TTTTTCAACT GCACCTGAAA GTCCATGGAA GGAAGCTCCT 
TATGAAGATG CTGCGGAAAA AACTGCCCTT GGAGGACTAT CATTTGACGC TTTCCTGTCA 
ATGTGGTCAC TGATGACACT ATTAGAACCG GCTCGGAGTG TGGAGAATTT GATTTACATT 
GGATTCCCGG GTGATCCATC GACTGCCATT CGTGTTACTA GGAGAAGACG TTTAGATCGC 
AAAAAGCAGC AATGTGAACG AAAAGTTTTC CAGTGCTTTG TTTTTGGACC TAATAATGCT 
GGAAAATCTG CATTGCTGAA TTGCTTTCTT GGAAGGTCAT ATACTGATAA CCAGGAATCA 
ACTACTGATG AGCGTTATGC AGTAAATATG GTCGATGAAT CGGGTGCTAA GAAAACTCTT 
ATCATGAGGG AAATTCCAGA AGATGGAGTT CAAGGGCTAT TTTCATCCAA GGAATCCTTA 
GCTGCATGTG ACATAGCTGT CTTCGTGTAT GACAGTTCTG ATGAGTCGTC ATGGAAGAGA 
GCGACCCAGT TGCTTGTAGA AGTTGCAAAT TATGGTGAGG CCACTGGGTA TGAGGTCCCT 
TGCCTCATGG TTTCAGCAAA AGACGATCTT GATTCATCTC CTATTTCCAT ACAAGAATCC 
ACCAGGATGA CACAAGATAT GGGAATAGAG CCTCCGGTAT CAATCAGCTC CAAATTGGGG 
GATTTCAACA ATTTATTCCG CAAAATCTTA ACTGCAGCAC AACATCCTCA TTTGAGCATT 
CCAGAGACCG AAGCAGGCAA AAGCCGCAAA CATTACAACA GGCTAATCAA CCGCTCTCTT 
ATGGCCGTTT CCATTGGTGC TGCTGCTGTG GTCGTTGGGC TGGCTGCATA CCGTGTGTAT 
GCCACAAGAA AGAGCTCGTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_198106.1
CDS	173..2119
Translation	MARYAAGAVDCPGSPKSVRIVVVGDKGTGKSSLIVAAATDSFPPNVPPVLPDYKLPIEFFPDGIPVTIVDTSSRPEDRDIVAEELKRADAVVLTYACDRPETLERLSEYWLPELRRLEVKIPIIVAGCKLDFRDDNNQVSLEQVMSPIMQQFREIETCIECSALKQLQAQEVFYYAQKTVLHPTGPLFDQDSQALKPRCVRALKRIFILCDHDRDGALSEAELNDFQVKCFHAPLQPSEIEGVKRVVQEKLPEGVNERGLTVTGFLFLHALFIEKGRLETTWTVLRKFGYNNDIRLAEELLPSAIFKRAPDQSFELTNAAIDFLKGMYMLFDDDQDNNLRPQEIEDLFSTAPESPWKEAPYEDAAEKTALGGLSFDAFLSMWSLMTLLEPARSVENLIYIGFPGDPSTAIRVTRRRRLDRKKQQCERKVFQCFVFGPNNAGKSALLNCFLGRSYTDNQESTTDERYAVNMVDESGAKKTLIMREIPEDGVQGLFSSKESLAACDIAVFVYDSSDESSWKRATQLLVEVANYGEATGYEVPCLMVSAKDDLDSSPISIQESTRMTQDMGIEPPVSISSKLGDFNNLFRKILTAAQHPHLSIPETEAGKSRKHYNRLINRSLMAVSIGAAAVVVGLAAYRVYATRKSSSA

Target ORF information:

RefSeq Version	NM_122636.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana MIRO-related GTP-ase 1 (MIRO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_122636.4

ORF Insert Sequence:

ATGGCGAGAT ACGCTGCTGG TGCGGTTGAT TGTCCTGGTT CTCCCAAATC TGTGCGAATC 
GTTGTGGTTG GTGATAAGGG CACTGGAAAA TCAAGCTTGA TTGTTGCTGC AGCCACTGAT 
TCTTTCCCTC CCAATGTCCC TCCTGTTTTG CCTGATTACA AGTTGCCTAT TGAATTCTTC 
CCTGATGGTA TACCCGTCAC CATTGTCGAC ACTTCCTCGA GGCCAGAAGA TAGGGACATT 
GTTGCTGAGG AATTGAAGCG CGCAGATGCA GTAGTTTTGA CATATGCCTG TGATCGACCT 
GAGACTCTCG AACGTTTGAG TGAATACTGG CTTCCAGAGC TTCGGCGATT AGAGGTAAAA 
ATTCCTATTA TCGTAGCAGG ATGTAAGCTT GACTTCAGAG ACGACAATAA CCAGGTCAGC 
CTCGAACAAG TGATGTCACC TATAATGCAG CAGTTTCGGG AGATAGAGAC TTGTATCGAG 
TGTTCTGCCC TGAAGCAACT CCAGGCACAA GAAGTTTTCT ATTATGCACA AAAGACTGTC 
CTTCACCCAA CAGGACCTCT GTTTGATCAA GATTCCCAGG CACTGAAACC CCGGTGTGTA 
AGGGCATTGA AGCGTATCTT TATACTCTGT GATCATGACA GAGATGGTGC TCTTAGCGAG 
GCTGAGTTAA ATGATTTTCA GGTCAAGTGT TTCCATGCAC CATTGCAGCC TTCTGAAATT 
GAAGGTGTTA AGAGGGTTGT GCAAGAAAAG TTGCCAGAAG GTGTGAATGA AAGAGGACTG 
ACAGTCACTG GCTTCCTATT TTTACATGCA CTTTTTATTG AGAAAGGGAG GCTTGAGACT 
ACGTGGACTG TACTCAGGAA GTTTGGGTAT AATAATGATA TAAGACTTGC TGAAGAATTG 
CTTCCATCTG CAATATTCAA GCGAGCTCCT GATCAGAGCT TTGAGTTGAC AAATGCAGCA 
ATCGATTTCC TGAAAGGAAT GTATATGTTG TTTGATGACG ACCAGGATAA CAATCTGAGA 
CCTCAAGAGA TCGAGGATCT TTTTTCAACT GCACCTGAAA GTCCATGGAA GGAAGCTCCT 
TATGAAGATG CTGCGGAAAA AACTGCCCTT GGAGGACTAT CATTTGACGC TTTCCTGTCA 
ATGTGGTCAC TGATGACACT ATTAGAACCG GCTCGGAGTG TGGAGAATTT GATTTACATT 
GGATTCCCGG GTGATCCATC GACTGCCATT CGTGTTACTA GGAGAAGACG TTTAGATCGC 
AAAAAGCAGC AATGTGAACG AAAAGTTTTC CAGTGCTTTG TTTTTGGACC TAATAATGCT 
GGAAAATCTG CATTGCTGAA TTGCTTTCTT GGAAGGTCAT ATACTGATAA CCAGGAATCA 
ACTACTGATG AGCGTTATGC AGTAAATATG GTCGATGAAT CGGGTGCTAA GAAAACTCTT 
ATCATGAGGG AAATTCCAGA AGATGGAGTT CAAGGGCTAT TTTCATCCAA GGAATCCTTA 
GCTGCATGTG ACATAGCTGT CTTCGTGTAT GACAGTTCTG ATGAGTCGTC ATGGAAGAGA 
GCGACCCAGT TGCTTGTAGA AGTTGCAAAT TATGGTGAGG CCACTGGGTA TGAGGTCCCT 
TGCCTCATGG TTTCAGCAAA AGACGATCTT GATTCATCTC CTATTTCCAT ACAAGAATCC 
ACCAGGATGA CACAAGATAT GGGAATAGAG CCTCCGGTAT CAATCAGCTC CAAATTGGGG 
GATTTCAACA ATTTATTCCG CAAAATCTTA ACTGCAGCAC AACATCCTCA TTTGAGCATT 
CCAGAGACCG AAGCAGGCAA AAGCCGCAAA CATTACAACA GGCTAATCAA CCGCTCTCTT 
ATGGCCGTTT CCATTGGTGC TGCTGCTGTG GTCGTTGGGC TGGCTGCATA CCGTGTGTAT 
GCCACAAGAA AGAGCTCGTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
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MIRO1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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