DGK1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGK1
Entrez Gene ID	830686
Full Name	diacylglycerol kinase1
Synonyms	ATDGK1,DIACYLGLYCEROL KINASE 1,F13G24.120,F13G24_120,diacylglycerol kinase1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001342967.1	NP_001331084.1	diacylglycerol kinase1
NM_001342968.1	NP_001331085.1	diacylglycerol kinase1
NM_120874.3	NP_196409.1	diacylglycerol kinase1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DGKE	XP_548222.2
Arabidopsis thaliana (thale cress)	DGK1	NP_196409.1
Gallus gallus (chicken)	DGKE	XP_001234226.3
Mus musculus (house mouse)	Dgke	NP_062378.1
Danio rerio (zebrafish)	dgke	NP_001165699.1
Drosophila melanogaster (fruit fly)	Dgkepsilon	NP_725228.1
Caenorhabditis elegans (roundworm)	dgk-2	NP_001024679.1
Xenopus tropicalis (tropical clawed frog)	dgke	XP_002937855.1
Pan troglodytes (chimpanzee)	DGKE	XP_001171847.2
Homo sapiens (human)	DGKE	NP_003638.1
Bos taurus (cattle)	DGKE	NP_001179859.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006768	XP_308977.4
Oryza sativa (rice)	Os12g0576900	NP_001067111.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DGK1 and DGK2 from cluster I in Arabidopsis thaliana possess amino-terminal hydrophobic segments that are sufficient to address them to endoplasmic reticulum membranes.
Title: The hydrophobic segment of Arabidopsis thaliana cluster I diacylglycerol kinases is sufficient to target the proteins to cell membranes.

The following DGK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb17862	NM_120874.3 Latest version!	Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb17862	NM_001342968.1 Latest version!	Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb40666	NM_001342967.1 Latest version!	Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGK1 ( NM_001342968.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331085.1 Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.

Price & Availability↑

CloneID	OAb17862
Clone ID Related Accession (Same CDS sequence)	NM_001342968.1 , NM_120874.3
Accession Version	NM_001342968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	diacylglycerol kinase1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGACGACG ATGGAGAATT GGGGATGTTC TTTCCTAGCT GGACCAGCAA GAATCCTATT 
GACACGGTTG AATCTCGTGG TTTAATGTTC TCTTGTTTTG TTGCTGCCCT TGTTGGTATA 
TTGACCATCG CCTACACTGC TTTTCAATGG CGAAGAAATA TTAATTTAAG TTGGACGAAA 
GCCATAGCCA GGTCAAAGAA AAATCCAAAG GCACGACATA AGGTTCCTGT TGCCCCACAT 
AGCTGGGAAC TCGACCCTAT AGCTCGTGCG AAAAACTTGA ACTGTTGTGT TTGCTTGAAG 
TCCATGTCGC CATCTCAGGC AATTGTAGCT TCAGAAAGTT TTTTCCACAG GTGCACAATC 
TGTGGAGCAG CAGCACATTT TAACTGTTCT TCAAGTGCGC CAAAAGATTG CAAATGTGTC 
TCCATGGTTG GATTTGAGCA TGTGGTGCAC CAGTGGGCAG TGCGGTGGAC AGAAGGGGCT 
GATCAGACTG ATGACTCCTC GTTTTGTAGC TACTGTGACG AGTCATGTAG TAGCTCCTTT 
CTTGGGGGTT CTCCTATATG GTGCTGCTTG TGGTGTCAAC GTCTTGTGCA CGTTGACTGT 
CACAGTAACA TGTCAAATGA AACTGGTGAC ATTTGTGATT TAGGCCCGCT TAGAAGGTTA 
ATATTGTGCC CACTCTACGT TAAGGAATTG ACGCGGAATC CCTCTGGAGG ATTTCTGAGC 
TCAATCACTC ATGGTGCTAA CGAACTTGCA TCTACCGCCC TTGCCAGTAT CAGGATTCAA 
AGCAAAAAAT ACAAGCAAAC TAATGAAACT TCAGCTGACA CTGGTAATAG TGGTAGCAAT 
TGCGATGAAT CCACAGAAAG CACAGCTGAT ACAGGTCCAA CTGTTAATGG CGCTCATGCC 
GTACTGGAAA ATTCTATCAG CGTTATGAAT GGGGATTCTT CTAACGGGGA CAGTGACAGC 
AATGGCAAGC TGGAAAAGAA GCCGAGTGTT AAAAGAACCG GGTCTTTTGG TCAGAAGGAA 
TATCATGCAC TAAGGTCGAA ACTTAAGTAT GAACTAGCTG ATTTGCCTTC AGATGCAAGA 
CCGTTGTTGG TTTTTATTAA CAAAAAGAGT GGTGCTCAAC GAGGTGATTC CCTTCGTCAA 
CGTCTTCATC TTCATCTAAA TCCTGTGCAG GTATTTGAAT TGAGTTCAGT GCAGGGACCA 
GAAGTGGGAC TTTTTCTCTT CAGGAAGGTT CCTCACTTTA GAGTTCTTGT TTGTGGTGGA 
GATGGCACCG CTGGTTGGGT ATTAGATGCC ATAGAGAAAC AGAATTTTAT TTCTCCTCCT 
GCAGTTGCTA TACTGCCTGC TGGAACCGGG AATGATCTAT CCCGAGTATT AAATTGGGGT 
GGTGGTTTGG GTTCTGTTGA GAGACAAGGA GGCTTGTCGA CAGTATTACA GAACATAGAG 
CATGCTGCAG TCACTGTCCT TGATCGTTGG AAAGTATCGA TTCTGAATCA ACAAGGAAAG 
CAACTCCAGC CGCCAAAATA TATGAACAAT TATATAGGGG TTGGGTGTGA TGCTAAGGTT 
GCCCTTGAGA TTCACAATCT ACGGGAGGAG AATCCAGAGA GATTTTATAG CCAGTTTATG 
AACAAAGTCC TCTATGCCAG AGAAGGTGCA AGGAGTATAA TGGACAGAAC ATTCGAAGAT 
TTCCCTTGGC AAGTTCGAGT TGAGGTGGAT GGTGTTGACA TCGAGGTTCC TGAGGATGCG 
GAAGGAATAC TGGTTGCAAA CATTGGAAGT TACATGGGAG GGGTGGATTT ATGGCAGAAT 
GAAGATGAAA CATATGAAAA CTTTGATCCG CAATCGATGC ACGACAAGAT AGTTGAAGTT 
GTGAGTATAT CTGGGACATG GCACCTTGGG AAACTTCAGG TCGGGTTGTC TCGTGCGAGA 
AGGCTAGCTC AAGGGTCAGC AGTCAAGATA CAACTTTGTG CGCCTTTACC AGTGCAAATT 
GATGGAGAGC CTTGGAATCA GCAACCATGT ACCTTAACCA TATCGCACCA TGGCCAGGCT 
TTCATGCTAA AGAGAGCCGC GGAAGAGCCA CTGGGTCACG CAGCAGCTAT AATCACAGAT 
GTTCTAGAGA ATGCAGAAAC CAATCAAGTG ATAAACGCTT CTCAGAAACG AACGCTGCTT 
CAAGAAATGG CTCTACGGCT AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331085.1
CDS	341..2527
Translation	MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTKAIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAIVASESFFHRCTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCSSSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGGFLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNGAHAVLENSISVMNGDSSNGDSDSNGKLEKKPSVKRTGSFGQKEYHALRSKLKYELADLPSDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHFRVLVCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVLQNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPLPVQIDGEPWNQQPCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLLQEMALRLT

Target ORF information:

RefSeq Version	NM_001342968.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342968.1

ORF Insert Sequence:

ATGGACGACG ATGGAGAATT GGGGATGTTC TTTCCTAGCT GGACCAGCAA GAATCCTATT 
GACACGGTTG AATCTCGTGG TTTAATGTTC TCTTGTTTTG TTGCTGCCCT TGTTGGTATA 
TTGACCATCG CCTACACTGC TTTTCAATGG CGAAGAAATA TTAATTTAAG TTGGACGAAA 
GCCATAGCCA GGTCAAAGAA AAATCCAAAG GCACGACATA AGGTTCCTGT TGCCCCACAT 
AGCTGGGAAC TCGACCCTAT AGCTCGTGCG AAAAACTTGA ACTGTTGTGT TTGCTTGAAG 
TCCATGTCGC CATCTCAGGC AATTGTAGCT TCAGAAAGTT TTTTCCACAG GTGCACAATC 
TGTGGAGCAG CAGCACATTT TAACTGTTCT TCAAGTGCGC CAAAAGATTG CAAATGTGTC 
TCCATGGTTG GATTTGAGCA TGTGGTGCAC CAGTGGGCAG TGCGGTGGAC AGAAGGGGCT 
GATCAGACTG ATGACTCCTC GTTTTGTAGC TACTGTGACG AGTCATGTAG TAGCTCCTTT 
CTTGGGGGTT CTCCTATATG GTGCTGCTTG TGGTGTCAAC GTCTTGTGCA CGTTGACTGT 
CACAGTAACA TGTCAAATGA AACTGGTGAC ATTTGTGATT TAGGCCCGCT TAGAAGGTTA 
ATATTGTGCC CACTCTACGT TAAGGAATTG ACGCGGAATC CCTCTGGAGG ATTTCTGAGC 
TCAATCACTC ATGGTGCTAA CGAACTTGCA TCTACCGCCC TTGCCAGTAT CAGGATTCAA 
AGCAAAAAAT ACAAGCAAAC TAATGAAACT TCAGCTGACA CTGGTAATAG TGGTAGCAAT 
TGCGATGAAT CCACAGAAAG CACAGCTGAT ACAGGTCCAA CTGTTAATGG CGCTCATGCC 
GTACTGGAAA ATTCTATCAG CGTTATGAAT GGGGATTCTT CTAACGGGGA CAGTGACAGC 
AATGGCAAGC TGGAAAAGAA GCCGAGTGTT AAAAGAACCG GGTCTTTTGG TCAGAAGGAA 
TATCATGCAC TAAGGTCGAA ACTTAAGTAT GAACTAGCTG ATTTGCCTTC AGATGCAAGA 
CCGTTGTTGG TTTTTATTAA CAAAAAGAGT GGTGCTCAAC GAGGTGATTC CCTTCGTCAA 
CGTCTTCATC TTCATCTAAA TCCTGTGCAG GTATTTGAAT TGAGTTCAGT GCAGGGACCA 
GAAGTGGGAC TTTTTCTCTT CAGGAAGGTT CCTCACTTTA GAGTTCTTGT TTGTGGTGGA 
GATGGCACCG CTGGTTGGGT ATTAGATGCC ATAGAGAAAC AGAATTTTAT TTCTCCTCCT 
GCAGTTGCTA TACTGCCTGC TGGAACCGGG AATGATCTAT CCCGAGTATT AAATTGGGGT 
GGTGGTTTGG GTTCTGTTGA GAGACAAGGA GGCTTGTCGA CAGTATTACA GAACATAGAG 
CATGCTGCAG TCACTGTCCT TGATCGTTGG AAAGTATCGA TTCTGAATCA ACAAGGAAAG 
CAACTCCAGC CGCCAAAATA TATGAACAAT TATATAGGGG TTGGGTGTGA TGCTAAGGTT 
GCCCTTGAGA TTCACAATCT ACGGGAGGAG AATCCAGAGA GATTTTATAG CCAGTTTATG 
AACAAAGTCC TCTATGCCAG AGAAGGTGCA AGGAGTATAA TGGACAGAAC ATTCGAAGAT 
TTCCCTTGGC AAGTTCGAGT TGAGGTGGAT GGTGTTGACA TCGAGGTTCC TGAGGATGCG 
GAAGGAATAC TGGTTGCAAA CATTGGAAGT TACATGGGAG GGGTGGATTT ATGGCAGAAT 
GAAGATGAAA CATATGAAAA CTTTGATCCG CAATCGATGC ACGACAAGAT AGTTGAAGTT 
GTGAGTATAT CTGGGACATG GCACCTTGGG AAACTTCAGG TCGGGTTGTC TCGTGCGAGA 
AGGCTAGCTC AAGGGTCAGC AGTCAAGATA CAACTTTGTG CGCCTTTACC AGTGCAAATT 
GATGGAGAGC CTTGGAATCA GCAACCATGT ACCTTAACCA TATCGCACCA TGGCCAGGCT 
TTCATGCTAA AGAGAGCCGC GGAAGAGCCA CTGGGTCACG CAGCAGCTAT AATCACAGAT 
GTTCTAGAGA ATGCAGAAAC CAATCAAGTG ATAAACGCTT CTCAGAAACG AACGCTGCTT 
CAAGAAATGG CTCTACGGCT AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGK1 ( NM_120874.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196409.1 Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.

Price & Availability↑

CloneID	OAb17862
Clone ID Related Accession (Same CDS sequence)	NM_001342968.1 , NM_120874.3
Accession Version	NM_120874.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	diacylglycerol kinase1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_120874.2.

ATGGACGACG ATGGAGAATT GGGGATGTTC TTTCCTAGCT GGACCAGCAA GAATCCTATT 
GACACGGTTG AATCTCGTGG TTTAATGTTC TCTTGTTTTG TTGCTGCCCT TGTTGGTATA 
TTGACCATCG CCTACACTGC TTTTCAATGG CGAAGAAATA TTAATTTAAG TTGGACGAAA 
GCCATAGCCA GGTCAAAGAA AAATCCAAAG GCACGACATA AGGTTCCTGT TGCCCCACAT 
AGCTGGGAAC TCGACCCTAT AGCTCGTGCG AAAAACTTGA ACTGTTGTGT TTGCTTGAAG 
TCCATGTCGC CATCTCAGGC AATTGTAGCT TCAGAAAGTT TTTTCCACAG GTGCACAATC 
TGTGGAGCAG CAGCACATTT TAACTGTTCT TCAAGTGCGC CAAAAGATTG CAAATGTGTC 
TCCATGGTTG GATTTGAGCA TGTGGTGCAC CAGTGGGCAG TGCGGTGGAC AGAAGGGGCT 
GATCAGACTG ATGACTCCTC GTTTTGTAGC TACTGTGACG AGTCATGTAG TAGCTCCTTT 
CTTGGGGGTT CTCCTATATG GTGCTGCTTG TGGTGTCAAC GTCTTGTGCA CGTTGACTGT 
CACAGTAACA TGTCAAATGA AACTGGTGAC ATTTGTGATT TAGGCCCGCT TAGAAGGTTA 
ATATTGTGCC CACTCTACGT TAAGGAATTG ACGCGGAATC CCTCTGGAGG ATTTCTGAGC 
TCAATCACTC ATGGTGCTAA CGAACTTGCA TCTACCGCCC TTGCCAGTAT CAGGATTCAA 
AGCAAAAAAT ACAAGCAAAC TAATGAAACT TCAGCTGACA CTGGTAATAG TGGTAGCAAT 
TGCGATGAAT CCACAGAAAG CACAGCTGAT ACAGGTCCAA CTGTTAATGG CGCTCATGCC 
GTACTGGAAA ATTCTATCAG CGTTATGAAT GGGGATTCTT CTAACGGGGA CAGTGACAGC 
AATGGCAAGC TGGAAAAGAA GCCGAGTGTT AAAAGAACCG GGTCTTTTGG TCAGAAGGAA 
TATCATGCAC TAAGGTCGAA ACTTAAGTAT GAACTAGCTG ATTTGCCTTC AGATGCAAGA 
CCGTTGTTGG TTTTTATTAA CAAAAAGAGT GGTGCTCAAC GAGGTGATTC CCTTCGTCAA 
CGTCTTCATC TTCATCTAAA TCCTGTGCAG GTATTTGAAT TGAGTTCAGT GCAGGGACCA 
GAAGTGGGAC TTTTTCTCTT CAGGAAGGTT CCTCACTTTA GAGTTCTTGT TTGTGGTGGA 
GATGGCACCG CTGGTTGGGT ATTAGATGCC ATAGAGAAAC AGAATTTTAT TTCTCCTCCT 
GCAGTTGCTA TACTGCCTGC TGGAACCGGG AATGATCTAT CCCGAGTATT AAATTGGGGT 
GGTGGTTTGG GTTCTGTTGA GAGACAAGGA GGCTTGTCGA CAGTATTACA GAACATAGAG 
CATGCTGCAG TCACTGTCCT TGATCGTTGG AAAGTATCGA TTCTGAATCA ACAAGGAAAG 
CAACTCCAGC CGCCAAAATA TATGAACAAT TATATAGGGG TTGGGTGTGA TGCTAAGGTT 
GCCCTTGAGA TTCACAATCT ACGGGAGGAG AATCCAGAGA GATTTTATAG CCAGTTTATG 
AACAAAGTCC TCTATGCCAG AGAAGGTGCA AGGAGTATAA TGGACAGAAC ATTCGAAGAT 
TTCCCTTGGC AAGTTCGAGT TGAGGTGGAT GGTGTTGACA TCGAGGTTCC TGAGGATGCG 
GAAGGAATAC TGGTTGCAAA CATTGGAAGT TACATGGGAG GGGTGGATTT ATGGCAGAAT 
GAAGATGAAA CATATGAAAA CTTTGATCCG CAATCGATGC ACGACAAGAT AGTTGAAGTT 
GTGAGTATAT CTGGGACATG GCACCTTGGG AAACTTCAGG TCGGGTTGTC TCGTGCGAGA 
AGGCTAGCTC AAGGGTCAGC AGTCAAGATA CAACTTTGTG CGCCTTTACC AGTGCAAATT 
GATGGAGAGC CTTGGAATCA GCAACCATGT ACCTTAACCA TATCGCACCA TGGCCAGGCT 
TTCATGCTAA AGAGAGCCGC GGAAGAGCCA CTGGGTCACG CAGCAGCTAT AATCACAGAT 
GTTCTAGAGA ATGCAGAAAC CAATCAAGTG ATAAACGCTT CTCAGAAACG AACGCTGCTT 
CAAGAAATGG CTCTACGGCT AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196409.1
CDS	529..2715
Translation	MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTKAIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAIVASESFFHRCTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCSSSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGGFLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNGAHAVLENSISVMNGDSSNGDSDSNGKLEKKPSVKRTGSFGQKEYHALRSKLKYELADLPSDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHFRVLVCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVLQNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPLPVQIDGEPWNQQPCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLLQEMALRLT

Target ORF information:

RefSeq Version	NM_120874.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_120874.3

ORF Insert Sequence:

ATGGACGACG ATGGAGAATT GGGGATGTTC TTTCCTAGCT GGACCAGCAA GAATCCTATT 
GACACGGTTG AATCTCGTGG TTTAATGTTC TCTTGTTTTG TTGCTGCCCT TGTTGGTATA 
TTGACCATCG CCTACACTGC TTTTCAATGG CGAAGAAATA TTAATTTAAG TTGGACGAAA 
GCCATAGCCA GGTCAAAGAA AAATCCAAAG GCACGACATA AGGTTCCTGT TGCCCCACAT 
AGCTGGGAAC TCGACCCTAT AGCTCGTGCG AAAAACTTGA ACTGTTGTGT TTGCTTGAAG 
TCCATGTCGC CATCTCAGGC AATTGTAGCT TCAGAAAGTT TTTTCCACAG GTGCACAATC 
TGTGGAGCAG CAGCACATTT TAACTGTTCT TCAAGTGCGC CAAAAGATTG CAAATGTGTC 
TCCATGGTTG GATTTGAGCA TGTGGTGCAC CAGTGGGCAG TGCGGTGGAC AGAAGGGGCT 
GATCAGACTG ATGACTCCTC GTTTTGTAGC TACTGTGACG AGTCATGTAG TAGCTCCTTT 
CTTGGGGGTT CTCCTATATG GTGCTGCTTG TGGTGTCAAC GTCTTGTGCA CGTTGACTGT 
CACAGTAACA TGTCAAATGA AACTGGTGAC ATTTGTGATT TAGGCCCGCT TAGAAGGTTA 
ATATTGTGCC CACTCTACGT TAAGGAATTG ACGCGGAATC CCTCTGGAGG ATTTCTGAGC 
TCAATCACTC ATGGTGCTAA CGAACTTGCA TCTACCGCCC TTGCCAGTAT CAGGATTCAA 
AGCAAAAAAT ACAAGCAAAC TAATGAAACT TCAGCTGACA CTGGTAATAG TGGTAGCAAT 
TGCGATGAAT CCACAGAAAG CACAGCTGAT ACAGGTCCAA CTGTTAATGG CGCTCATGCC 
GTACTGGAAA ATTCTATCAG CGTTATGAAT GGGGATTCTT CTAACGGGGA CAGTGACAGC 
AATGGCAAGC TGGAAAAGAA GCCGAGTGTT AAAAGAACCG GGTCTTTTGG TCAGAAGGAA 
TATCATGCAC TAAGGTCGAA ACTTAAGTAT GAACTAGCTG ATTTGCCTTC AGATGCAAGA 
CCGTTGTTGG TTTTTATTAA CAAAAAGAGT GGTGCTCAAC GAGGTGATTC CCTTCGTCAA 
CGTCTTCATC TTCATCTAAA TCCTGTGCAG GTATTTGAAT TGAGTTCAGT GCAGGGACCA 
GAAGTGGGAC TTTTTCTCTT CAGGAAGGTT CCTCACTTTA GAGTTCTTGT TTGTGGTGGA 
GATGGCACCG CTGGTTGGGT ATTAGATGCC ATAGAGAAAC AGAATTTTAT TTCTCCTCCT 
GCAGTTGCTA TACTGCCTGC TGGAACCGGG AATGATCTAT CCCGAGTATT AAATTGGGGT 
GGTGGTTTGG GTTCTGTTGA GAGACAAGGA GGCTTGTCGA CAGTATTACA GAACATAGAG 
CATGCTGCAG TCACTGTCCT TGATCGTTGG AAAGTATCGA TTCTGAATCA ACAAGGAAAG 
CAACTCCAGC CGCCAAAATA TATGAACAAT TATATAGGGG TTGGGTGTGA TGCTAAGGTT 
GCCCTTGAGA TTCACAATCT ACGGGAGGAG AATCCAGAGA GATTTTATAG CCAGTTTATG 
AACAAAGTCC TCTATGCCAG AGAAGGTGCA AGGAGTATAA TGGACAGAAC ATTCGAAGAT 
TTCCCTTGGC AAGTTCGAGT TGAGGTGGAT GGTGTTGACA TCGAGGTTCC TGAGGATGCG 
GAAGGAATAC TGGTTGCAAA CATTGGAAGT TACATGGGAG GGGTGGATTT ATGGCAGAAT 
GAAGATGAAA CATATGAAAA CTTTGATCCG CAATCGATGC ACGACAAGAT AGTTGAAGTT 
GTGAGTATAT CTGGGACATG GCACCTTGGG AAACTTCAGG TCGGGTTGTC TCGTGCGAGA 
AGGCTAGCTC AAGGGTCAGC AGTCAAGATA CAACTTTGTG CGCCTTTACC AGTGCAAATT 
GATGGAGAGC CTTGGAATCA GCAACCATGT ACCTTAACCA TATCGCACCA TGGCCAGGCT 
TTCATGCTAA AGAGAGCCGC GGAAGAGCCA CTGGGTCACG CAGCAGCTAT AATCACAGAT 
GTTCTAGAGA ATGCAGAAAC CAATCAAGTG ATAAACGCTT CTCAGAAACG AACGCTGCTT 
CAAGAAATGG CTCTACGGCT AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGK1 ( NM_001342967.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331084.1 Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.

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CloneID	OAb40666
Clone ID Related Accession (Same CDS sequence)	NM_001342967.1
Accession Version	NM_001342967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	diacylglycerol kinase1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGACGACG ATGGAGAATT GGGGATGTTC TTTCCTAGCT GGACCAGCAA GAATCCTATT 
GACACGGTTG AATCTCGTGG TTTAATGTTC TCTTGTTTTG TTGCTGCCCT TGTTGGTATA 
TTGACCATCG CCTACACTGC TTTTCAATGG CGAAGAAATA TTAATTTAAG TTGGACGAAA 
GCCATAGCCA GGTCAAAGAA AAATCCAAAG GCACGACATA AGGTTCCTGT TGCCCCACAT 
AGCTGGGAAC TCGACCCTAT AGCTCGTGCG AAAAACTTGA ACTGTTGTGT TTGCTTGAAG 
TCCATGTCGC CATCTCAGGC AATTGTAGCT TCAGAAAGTT TTTTCCACAG GTGCACAATC 
TGTGGAGCAG CAGCACATTT TAACTGTTCT TCAAGTGCGC CAAAAGATTG CAAATGTGTC 
TCCATGGTTG GATTTGAGCA TGTGGTGCAC CAGTGGGCAG TGCGGTGGAC AGAAGGGGCT 
GATCAGACTG ATGACTCCTC GTTTTGTAGC TACTGTGACG AGTCATGTAG TAGCTCCTTT 
CTTGGGGGTT CTCCTATATG GTGCTGCTTG TGGTGTCAAC GTCTTGTGCA CGTTGACTGT 
CACAGTAACA TGTCAAATGA AACTGGTGAC ATTTGTGATT TAGGCCCGCT TAGAAGGTTA 
ATATTGTGCC CACTCTACGT TAAGGAATTG ACGCGGAATC CCTCTGGAGG ATTTCTGAGC 
TCAATCACTC ATGGTGCTAA CGAACTTGCA TCTACCGCCC TTGCCAGTAT CAGGATTCAA 
AGCAAAAAAT ACAAGCAAAC TAATGAAACT TCAGCTGACA CTGGTAATAG TGGTAGCAAT 
TGCGATGAAT CCACAGAAAG CACAGCTGAT ACAGGTCCAA CTGTTAATGG CGCTCATGCC 
GTACTGGAAA ATTCTATCAG CGTTATGAAT GGGGATTCTT CTAACGGGGA CAGTGACAGC 
AATGGCAAGC TGGAAAAGAA GCCGAGTGTT AAAAGAACCG GGTCTTTTGG TCAGAAGGAA 
TATCATGCAC TAAGGTCGAA ACTTAAGTAT GAACTAGCTG ATTTGCCTTC AGATGCAAGA 
CCGTTGTTGG TTTTTATTAA CAAAAAGAGT GGTGCTCAAC GAGGTGATTC CCTTCGTCAA 
CGTCTTCATC TTCATCTAAA TCCTGTGCAG GTATTTGAAT TGAGTTCAGT GCAGGGACCA 
GAAGTGGGAC TTTTTCTCTT CAGGAAGGTT CCTCACTTTA GAGTTCTTGT TTGTGGTGGA 
GATGGCACCG CTGGTTGGGT ATTAGATGCC ATAGAGAAAC AGAATTTTAT TTCTCCTCCT 
GCAGTTGCTA TACTGCCTGC TGGAACCGGG AATGATCTAT CCCGAGTATT AAATTGGGGT 
GGTGGTTTGG GTTCTGTTGA GAGACAAGGA GGCTTGTCGA CAGTATTACA GAACATAGAG 
CATGCTGCAG TCACTGTCCT TGATCGTTGG AAAGTATCGA TTCTGAATCA ACAAGGAAAG 
CAACTCCAGC CGCCAAAATA TATGAACAAT TATATAGGGG TTGGGTGTGA TGCTAAGGTT 
GCCCTTGAGA TTCACAATCT ACGGGAGGAG AATCCAGAGA GATTTTATAG CCAGTTTATG 
AACAAAGTCC TCTATGCCAG AGAAGGTGCA AGGAGTATAA TGGACAGAAC ATTCGAAGAT 
TTCCCTTGGC AAGTTCGAGT TGAGGTGGAT GGTGTTGACA TCGAGGTTCC TGAGGATGCG 
GAAGGAATAC TGGTTGCAAA CATTGGAAGT TACATGGGAG GGGTGGATTT ATGGCAGAAT 
GAAGATGAAA CATATGAAAA CTTTGATCCG CAATCGATGC ACGACAAGAT AGTTGAAGTT 
GTGAGTATAT CTGGGACATG GCACCTTGGG AAACTTCAGG TCGGGTTGTC TCGTGCGAGA 
AGGCTAGCTC AAGGGTCAGC AGTCAAGATA CAACTTTGTG CGCCTTTACC AGTGCAAATT 
GATGGAGAGC CTTGGAATCA GCAACCATGT ACCTTAACCA TATCGCACCA TGGCCAGGTT 
CTTCTCTCCA TTTCCCTAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331084.1
CDS	529..2592
Translation	MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTKAIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAIVASESFFHRCTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCSSSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGGFLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNGAHAVLENSISVMNGDSSNGDSDSNGKLEKKPSVKRTGSFGQKEYHALRSKLKYELADLPSDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHFRVLVCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVLQNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPLPVQIDGEPWNQQPCTLTISHHGQVLLSISLT

Target ORF information:

RefSeq Version	NM_001342967.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana diacylglycerol kinase1 (DGK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342967.1

ORF Insert Sequence:

ATGGACGACG ATGGAGAATT GGGGATGTTC TTTCCTAGCT GGACCAGCAA GAATCCTATT 
GACACGGTTG AATCTCGTGG TTTAATGTTC TCTTGTTTTG TTGCTGCCCT TGTTGGTATA 
TTGACCATCG CCTACACTGC TTTTCAATGG CGAAGAAATA TTAATTTAAG TTGGACGAAA 
GCCATAGCCA GGTCAAAGAA AAATCCAAAG GCACGACATA AGGTTCCTGT TGCCCCACAT 
AGCTGGGAAC TCGACCCTAT AGCTCGTGCG AAAAACTTGA ACTGTTGTGT TTGCTTGAAG 
TCCATGTCGC CATCTCAGGC AATTGTAGCT TCAGAAAGTT TTTTCCACAG GTGCACAATC 
TGTGGAGCAG CAGCACATTT TAACTGTTCT TCAAGTGCGC CAAAAGATTG CAAATGTGTC 
TCCATGGTTG GATTTGAGCA TGTGGTGCAC CAGTGGGCAG TGCGGTGGAC AGAAGGGGCT 
GATCAGACTG ATGACTCCTC GTTTTGTAGC TACTGTGACG AGTCATGTAG TAGCTCCTTT 
CTTGGGGGTT CTCCTATATG GTGCTGCTTG TGGTGTCAAC GTCTTGTGCA CGTTGACTGT 
CACAGTAACA TGTCAAATGA AACTGGTGAC ATTTGTGATT TAGGCCCGCT TAGAAGGTTA 
ATATTGTGCC CACTCTACGT TAAGGAATTG ACGCGGAATC CCTCTGGAGG ATTTCTGAGC 
TCAATCACTC ATGGTGCTAA CGAACTTGCA TCTACCGCCC TTGCCAGTAT CAGGATTCAA 
AGCAAAAAAT ACAAGCAAAC TAATGAAACT TCAGCTGACA CTGGTAATAG TGGTAGCAAT 
TGCGATGAAT CCACAGAAAG CACAGCTGAT ACAGGTCCAA CTGTTAATGG CGCTCATGCC 
GTACTGGAAA ATTCTATCAG CGTTATGAAT GGGGATTCTT CTAACGGGGA CAGTGACAGC 
AATGGCAAGC TGGAAAAGAA GCCGAGTGTT AAAAGAACCG GGTCTTTTGG TCAGAAGGAA 
TATCATGCAC TAAGGTCGAA ACTTAAGTAT GAACTAGCTG ATTTGCCTTC AGATGCAAGA 
CCGTTGTTGG TTTTTATTAA CAAAAAGAGT GGTGCTCAAC GAGGTGATTC CCTTCGTCAA 
CGTCTTCATC TTCATCTAAA TCCTGTGCAG GTATTTGAAT TGAGTTCAGT GCAGGGACCA 
GAAGTGGGAC TTTTTCTCTT CAGGAAGGTT CCTCACTTTA GAGTTCTTGT TTGTGGTGGA 
GATGGCACCG CTGGTTGGGT ATTAGATGCC ATAGAGAAAC AGAATTTTAT TTCTCCTCCT 
GCAGTTGCTA TACTGCCTGC TGGAACCGGG AATGATCTAT CCCGAGTATT AAATTGGGGT 
GGTGGTTTGG GTTCTGTTGA GAGACAAGGA GGCTTGTCGA CAGTATTACA GAACATAGAG 
CATGCTGCAG TCACTGTCCT TGATCGTTGG AAAGTATCGA TTCTGAATCA ACAAGGAAAG 
CAACTCCAGC CGCCAAAATA TATGAACAAT TATATAGGGG TTGGGTGTGA TGCTAAGGTT 
GCCCTTGAGA TTCACAATCT ACGGGAGGAG AATCCAGAGA GATTTTATAG CCAGTTTATG 
AACAAAGTCC TCTATGCCAG AGAAGGTGCA AGGAGTATAA TGGACAGAAC ATTCGAAGAT 
TTCCCTTGGC AAGTTCGAGT TGAGGTGGAT GGTGTTGACA TCGAGGTTCC TGAGGATGCG 
GAAGGAATAC TGGTTGCAAA CATTGGAAGT TACATGGGAG GGGTGGATTT ATGGCAGAAT 
GAAGATGAAA CATATGAAAA CTTTGATCCG CAATCGATGC ACGACAAGAT AGTTGAAGTT 
GTGAGTATAT CTGGGACATG GCACCTTGGG AAACTTCAGG TCGGGTTGTC TCGTGCGAGA 
AGGCTAGCTC AAGGGTCAGC AGTCAAGATA CAACTTTGTG CGCCTTTACC AGTGCAAATT 
GATGGAGAGC CTTGGAATCA GCAACCATGT ACCTTAACCA TATCGCACCA TGGCCAGGTT 
CTTCTCTCCA TTTCCCTAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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DGK1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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