GLU1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLU1
Entrez Gene ID	830292
Full Name	glutamate synthase 1
Synonyms	F21E1.60,F21E1_60,FD-GOGAT,FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE,FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE 1,GLS1,GLUS,GLUTAMATE SYNTHASE,GLUTAMATE SYNTHASE 1,glutamate synthase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_120496.3	NP_568134.1	glutamate synthase 1
NM_180432.2	NP_850763.1	glutamate synthase 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	GLU2	NP_181655.1
Oryza sativa (rice)	Os07g0658400	NP_001060520.1
Arabidopsis thaliana (thale cress)	GLU1	NP_568134.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

glu1-2 mutant transcriptome was characterised by an extensive secondary response including the downregulation of photosynthesis-related pathways and the induction of genes and pathways involved in the plant response to a multitude of stresses.
Title: Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome.

GLU1 interacts with SHMT1 and this interaction is necessary for photorespiratory serine hydroxymethyltransferase (SHMT) activity.
Title: Arabidopsis photorespiratory serine hydroxymethyltransferase activity requires the mitochondrial accumulation of ferredoxin-dependent glutamate synthase.

Expression of GLU1 in mesophyll cells implies that Fd-GOGAT assimilates photorespiratory and primary ammonium. GLU1 expression in vascular cells indicates that Fd-GOGAT provides amino acids for nitrogen translocation.
Title: Expression of a ferredoxin-dependent glutamate synthase gene in mesophyll and vascular cells and functions of the enzyme in ammonium assimilation in Nicotiana tabacum (L.).

The following GLU1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLU1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb32128	NM_120496.3 Latest version!	Arabidopsis thaliana glutamate synthase 1 (GLU1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OAb32130	NM_180432.2 Latest version!	Arabidopsis thaliana glutamate synthase 1 (GLU1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLU1 ( NM_120496.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_568134.1 Arabidopsis thaliana glutamate synthase 1 (GLU1), mRNA.

Price & Availability↑

CloneID	OAb32128
Clone ID Related Accession (Same CDS sequence)	NM_120496.3
Accession Version	NM_120496.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	glutamate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_120496.2.

ATGGCGATGC AATCTCTTTC CCCTGTTCCT AAGCTTCTCT CCACAACACC AAGCTCTGTT 
CTTTCTTCTG ACAAGAACTT CTTCTTCGTC GATTTCGTTG GATTATACTG TAAGTCCAAG 
AGGACCAGAC GCAGACTTCG TGGAGACTCT TCCTCTTCCT CACGCTCTTC TTCTTCTCTC 
TCTCGTCTTT CCTCTGTTCG TGCCGTTATC GACCTTGAAC GTGTTCATGG CGTCTCTGAA 
AAGGATCTCT CCTCTCCTTC AGCTTTAAGA CCTCAGGTTG CTAATTTGGA GGATATATTG 
TCTGAAAGAG GAGCTTGTGG AGTTGGGTTT ATAGCAAACT TAGATAACAT ACCTTCACAT 
GGAGTTGTCA AAGATGCTCT TATTGCTCTT GGGTGTATGG AACATCGTGG AGGTTGTGGA 
GCAGACAATG ATTCTGGTGA TGGCTCTGGT CTTATGTCTT CCATTCCTTG GGATTTCTTT 
AACGTCTGGG CCAAGGAACA AAGTCTTGCT CCTTTTGATA AGTTGCATAC TGGTGTTGGC 
ATGATCTTTC TTCCACAAGA TGATACCTTT ATGCAAGAAG CCAAGCAAGT TATTGAAAAC 
ATATTTGAGA AAGAAGGATT ACAAGTTCTT GGGTGGAGGG AAGTTCCTGT TAATGTTCCT 
ATAGTTGGTA AAAATGCTAG GGAGACAATG CCTAACATTC AACAAGTGTT TGTGAAAATC 
GCAAAGGAAG ATAGTACTGA TGATATTGAA AGGGAACTTT ACATCTGCCG GAAACTAATC 
GAAAGGGCCG TAGCTACTGA GAGTTGGGGA ACAGAGCTTT ACTTCTGTTC ACTGTCCAAT 
CAAACCATAG TGTACAAGGG CATGCTTCGA TCTGAAGCTC TTGGATTGTT TTATCTAGAT 
CTTCAGAATG AGCTTTATGA GTCTCCTTTT GCTATTTATC ATCGAAGGTA CAGTACAAAC 
ACTAGTCCTA GGTGGCCTCT TGCTCAACCA ATGAGGTTTC TTGGACATAA CGGGGAGATC 
AATACCATTC AGGGGAACTT AAATTGGATG CAGTCTCGAG AAGCTTCATT GAAGGCTGCT 
GTTTGGAATG GCCGTGAAAA TGAAATTCGT CCATTTGGTA ATCCCAGGGG TTCAGACTCT 
GCTAATCTTG ATAGTGCTGC AGAAATCATG ATTAGAAGTG GAAGAACACC AGAGGAAGCT 
CTAATGATTC TTGTCCCTGA GGCATACAAG AATCATCCAA CCTTATCTGT CAAATATCCT 
GAGGTTGTAG ATTTCTATGA CTACTACAAA GGACAAATGG AGGCTTGGGA TGGTCCTGCC 
TTACTTTTGT TCAGTGATGG AAAAACAGTT GGGGCTTGTC TTGACCGTAA TGGCCTTCGT 
CCTGCTCGAT ATTGGCGGAC TAGTGACAAT TTCGTCTATG TTGCATCTGA GGTCGGAGTT 
GTACCAGTTG ATGAGGCAAA AGTCACAATG AAAGGCCGTC TAGGACCTGG AATGATGATT 
GCTGTTGACC TTGTGAATGG CCAGGTATAT GAGAATACAG AGGTCAAGAA GAGAATATCT 
TCATTTAATC CATATGGAAA ATGGATTAAA GAAAACTCCC GGTTCTTGAA GCCTGTGAAT 
TTCAAATCCT CAACTGTCAT GGAAAATGAA GAAATCCTAA GAAGCCAACA AGCATTTGGT 
TATTCAAGTG AGGATGTGCA AATGGTTATT GAGTCTATGG CTTCTCAAGG AAAGGAACCA 
ACCTTCTGCA TGGGCGACGA TATTCCGCTG GCAGGATTGT CTCAAAGACC GCATATGCTT 
TATGATTATT TCAAACAAAG ATTTGCACAG GTTACAAACC CTGCCATTGA CCCCCTTAGG 
GAAGGTTTGG TTATGTCTCT TGAAGTAAAT ATTGGAAAAC GTGGAAATAT ATTGGAGCTT 
GGACCTGAGA ATGCCTCGCA GGTTATTCTG TCTAACCCTG TGTTAAATGA AGGAGCGTTA 
GAGGAGTTAA TGAAGGATCA ATACTTAAAA CCAAAGGTTC TGTCCACATA TTTCGATATA 
AGAAAAGGCG TTGAAGGTTC CTTGCAAAAG GCTCTATATT ATCTTTGTGA AGCAGCTGAT 
GATGCTGTCC GAAGTGGCTC TCAGCTTCTC GTTCTTTCAG ACCGATCCGA TAGACTGGAA 
CCAACCAGGC CTTCAATTCC AATAATGTTA GCTGTTGGCG CTGTCCATCA ACATCTTATT 
CAGAACGGCT TGCGTATGTC AGCTTCTATT GTTGCTGATA CCGCCCAATG CTTCAGCACA 
CATCATTTTG CTTGTTTGGT TGGATATGGT GCAAGTGCTG TATGCCCATA CTTGGCACTG 
GAGACATGTA GGCAATGGCG CTTAAGTAAC AAAACTGTGG CCTTCATGCG TAACGGGAAA 
ATTCCTACTG TAACCATTGA GCAAGCTCAG AAGAACTACA CTAAGGCGGT TAATGCAGGG 
CTTCTTAAAA TTCTTTCTAA GATGGGAATC TCATTGCTTT CAAGTTATTG TGGTGCTCAG 
ATATTTGAGA TATATGGTTT GGGACAGGAT GTTGTTGATC TTGCATTCAC TGGAAGTGTG 
TCAAAAATCA GTGGACTCAC CTTTGATGAG TTGGCAAGAG AGACATTGTC TTTCTGGGTG 
AAGGCCTTTT CTGAGGATAC AACTAAGCGA TTAGAAAATT TTGGGTTTAT TCAATTCAGG 
CCTGGAGGTG AGTATCATTC AAACAACCCA GAGATGTCAA AGTTGCTTCA CAAGGCTGTC 
CGTGAAAAGA GTGAAACTGC ATATGCAGTC TATCAACAGC ATCTCTCTAA CAGACCTGTT 
AATGTCCTCC GTGACCTGCT TGAGTTCAAG AGTGATCGTG CACCGATCCC AGTAGGGAAA 
GTAGAACCGG CCGTTGCTAT TGTTCAGAGA TTTTGTACTG GTGGAATGTC ACTTGGTGCT 
ATTTCAAGAG AGACTCATGA AGCTATTGCT ATTGCAATGA ATAGGATTGG TGGGAAATCA 
AACTCTGGAG AAGGTGGAGA GGATCCTATC CGTTGGAAGC CACTTACAGA TGTGGTTGAT 
GGATATTCAC CAACACTACC ACATCTCAAA GGTCTTCAAA ACGGCGATAT TGCAACAAGT 
GCTATCAAGC AGGTTGCTTC AGGGCGTTTT GGAGTCACAC CAACGTTCTT GGTCAATGCA 
GATCAATTGG AAATCAAAGT TGCACAAGGT GCCAAGCCTG GGGAAGGTGG TCAGCTTCCT 
GGAAAGAAAG TTAGTGCGTA TATCGCTAGG CTAAGAAGCT CTAAACCTGG TGTTCCGCTT 
ATATCTCCGC CTCCTCACCA CGACATTTAC TCTATTGAGG ATCTTGCTCA GTTGATCTTT 
GATCTACATC AGATTAATCC AAATGCAAAA GTATCAGTCA AGCTAGTTGC AGAAGCTGGA 
ATCGGAACTG TTGCTTCAGG AGTTGCAAAG GGTAACGCTG ATATCATCCA GATATCAGGC 
CATGATGGTG GAACCGGGGC TAGTCCAATA AGCTCCATAA AACATGCTGG TGGACCATGG 
GAACTTGGAC TAACAGAAAC TCACCAAACA CTTATCGCAA ATGGACTAAG AGAAAGAGTC 
ATTTTAAGAG TCGATGGAGG CTTAAAGAGT GGTGTTGATG TTCTAATGGC TGCAGCTATG 
GGTGCTGATG AATACGGATT CGGTTCCTTG GCAATGATTG CTACTGGTTG TGTTATGGCT 
CGTATTTGCC ACACTAATAA TTGCCCAGTG GGTGTAGCAA GTCAGAGAGA AGAATTACGT 
GCAAGATTCC CTGGTGTACC TGGTGATCTT GTCAACTACT TCTTATACGT AGCAGAAGAG 
GTGAGAGGTA TCTTAGCACA GTTGGGATAC AACAGTTTAG ATGACATCAT TGGACGAACA 
GAGTTACTGA GACCACGAGA CATTTCGCTA GTTAAAACTC AACATCTCGA TCTGAGTTAT 
CTTCTTTCGT CTGTTGGAAC ACCTTCATTG AGCAGTACTG AAATCAGAAA GCAGGAAGTT 
CATACAAATG GACCTGTTCT CGACGACGAT ATTCTTGCAG ATCCATTGGT GATTGATGCA 
ATAGAGAACG AAAAAGTGGT TGAGAAAACC GTCAAAATAT GCAACGTAGA CCGTGCGGCT 
TGTGGTCGTG TTGCTGGTGT TATTGCAAAG AAGTATGGAG ACACTGGTTT TGCAGGACAA 
GTGAACCTAA CTTTCTTAGG GAGCGCGGGA CAGTCGTTTG GGTGCTTTTT GATTCCCGGT 
ATGAACATCC GGCTCATAGG AGAGTCAAAT GACTACGTTG GAAAGGGAAT GGCTGGTGGT 
GAAATAGTAG TAACTCCTGT GGAAAAAATC GGGTTTGTGC CTGAGGAAGC AACGATAGTC 
GGGAACACTT GCTTGTATGG TGCAACAGGT GGTCAGATAT TCGCTAGAGG CAAAGCTGGA 
GAGAGATTTG CAGTGAGAAA CTCACTCGCT GAAGCAGTAG TTGAAGGCAC TGGAGACCAT 
TGCTGTGAGT ACATGACTGG TGGCTGTGTA GTCGTGCTTG GAAAGGTGGG AAGAAACGTT 
GCTGCTGGTA TGACAGGAGG GTTAGCTTAC CTTCTTGATG AAGACGACAC TCTTCTTCCT 
AAGATTAACA GAGAGATAGT GAAGATCCAA AGAGTAACTG CGCCTGCAGG GGAATTGCAG 
CTGAAGAGCT TAATTGAAGC ACATGTGGAA AAAACCGGAA GCAGCAAAGG CGCAACGATT 
CTGAATGAGT GGGAAAAGTA TCTACCTCTC TTCTGGCAAC TGGTTCCACC GAGTGAGGAA 
GACACTCCTG AAGCTTCTGC TGCTTACGTA AGAACATCCA CCGGGGAAGT CACATTTCAA 
TCGGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_568134.1
CDS	247..5115
Translation	MAMQSLSPVPKLLSTTPSSVLSSDKNFFFVDFVGLYCKSKRTRRRLRGDSSSSSRSSSSLSRLSSVRAVIDLERVHGVSEKDLSSPSALRPQVANLEDILSERGACGVGFIANLDNIPSHGVVKDALIALGCMEHRGGCGADNDSGDGSGLMSSIPWDFFNVWAKEQSLAPFDKLHTGVGMIFLPQDDTFMQEAKQVIENIFEKEGLQVLGWREVPVNVPIVGKNARETMPNIQQVFVKIAKEDSTDDIERELYICRKLIERAVATESWGTELYFCSLSNQTIVYKGMLRSEALGLFYLDLQNELYESPFAIYHRRYSTNTSPRWPLAQPMRFLGHNGEINTIQGNLNWMQSREASLKAAVWNGRENEIRPFGNPRGSDSANLDSAAEIMIRSGRTPEEALMILVPEAYKNHPTLSVKYPEVVDFYDYYKGQMEAWDGPALLLFSDGKTVGACLDRNGLRPARYWRTSDNFVYVASEVGVVPVDEAKVTMKGRLGPGMMIAVDLVNGQVYENTEVKKRISSFNPYGKWIKENSRFLKPVNFKSSTVMENEEILRSQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAGLSQRPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILELGPENASQVILSNPVLNEGALEELMKDQYLKPKVLSTYFDIRKGVEGSLQKALYYLCEAADDAVRSGSQLLVLSDRSDRLEPTRPSIPIMLAVGAVHQHLIQNGLRMSASIVADTAQCFSTHHFACLVGYGASAVCPYLALETCRQWRLSNKTVAFMRNGKIPTVTIEQAQKNYTKAVNAGLLKILSKMGISLLSSYCGAQIFEIYGLGQDVVDLAFTGSVSKISGLTFDELARETLSFWVKAFSEDTTKRLENFGFIQFRPGGEYHSNNPEMSKLLHKAVREKSETAYAVYQQHLSNRPVNVLRDLLEFKSDRAPIPVGKVEPAVAIVQRFCTGGMSLGAISRETHEAIAIAMNRIGGKSNSGEGGEDPIRWKPLTDVVDGYSPTLPHLKGLQNGDIATSAIKQVASGRFGVTPTFLVNADQLEIKVAQGAKPGEGGQLPGKKVSAYIARLRSSKPGVPLISPPPHHDIYSIEDLAQLIFDLHQINPNAKVSVKLVAEAGIGTVASGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIANGLRERVILRVDGGLKSGVDVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNYFLYVAEEVRGILAQLGYNSLDDIIGRTELLRPRDISLVKTQHLDLSYLLSSVGTPSLSSTEIRKQEVHTNGPVLDDDILADPLVIDAIENEKVVEKTVKICNVDRAACGRVAGVIAKKYGDTGFAGQVNLTFLGSAGQSFGCFLIPGMNIRLIGESNDYVGKGMAGGEIVVTPVEKIGFVPEEATIVGNTCLYGATGGQIFARGKAGERFAVRNSLAEAVVEGTGDHCCEYMTGGCVVVLGKVGRNVAAGMTGGLAYLLDEDDTLLPKINREIVKIQRVTAPAGELQLKSLIEAHVEKTGSSKGATILNEWEKYLPLFWQLVPPSEEDTPEASAAYVRTSTGEVTFQSA

Target ORF information:

RefSeq Version	NM_120496.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana glutamate synthase 1 (GLU1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_120496.3

ORF Insert Sequence:

ATGGCGATGC AATCTCTTTC CCCTGTTCCT AAGCTTCTCT CCACAACACC AAGCTCTGTT 
CTTTCTTCTG ACAAGAACTT CTTCTTCGTC GATTTCGTTG GATTATACTG TAAGTCCAAG 
AGGACCAGAC GCAGACTTCG TGGAGACTCT TCCTCTTCCT CACGCTCTTC TTCTTCTCTC 
TCTCGTCTTT CCTCTGTTCG TGCCGTTATC GACCTTGAAC GTGTTCATGG CGTCTCTGAA 
AAGGATCTCT CCTCTCCTTC AGCTTTAAGA CCTCAGGTTG CTAATTTGGA GGATATATTG 
TCTGAAAGAG GAGCTTGTGG AGTTGGGTTT ATAGCAAACT TAGATAACAT ACCTTCACAT 
GGAGTTGTCA AAGATGCTCT TATTGCTCTT GGGTGTATGG AACATCGTGG AGGTTGTGGA 
GCAGACAATG ATTCTGGTGA TGGCTCTGGT CTTATGTCTT CCATTCCTTG GGATTTCTTT 
AACGTCTGGG CCAAGGAACA AAGTCTTGCT CCTTTTGATA AGTTGCATAC TGGTGTTGGC 
ATGATCTTTC TTCCACAAGA TGATACCTTT ATGCAAGAAG CCAAGCAAGT TATTGAAAAC 
ATATTTGAGA AAGAAGGATT ACAAGTTCTT GGGTGGAGGG AAGTTCCTGT TAATGTTCCT 
ATAGTTGGTA AAAATGCTAG GGAGACAATG CCTAACATTC AACAAGTGTT TGTGAAAATC 
GCAAAGGAAG ATAGTACTGA TGATATTGAA AGGGAACTTT ACATCTGCCG GAAACTAATC 
GAAAGGGCCG TAGCTACTGA GAGTTGGGGA ACAGAGCTTT ACTTCTGTTC ACTGTCCAAT 
CAAACCATAG TGTACAAGGG CATGCTTCGA TCTGAAGCTC TTGGATTGTT TTATCTAGAT 
CTTCAGAATG AGCTTTATGA GTCTCCTTTT GCTATTTATC ATCGAAGGTA CAGTACAAAC 
ACTAGTCCTA GGTGGCCTCT TGCTCAACCA ATGAGGTTTC TTGGACATAA CGGGGAGATC 
AATACCATTC AGGGGAACTT AAATTGGATG CAGTCTCGAG AAGCTTCATT GAAGGCTGCT 
GTTTGGAATG GCCGTGAAAA TGAAATTCGT CCATTTGGTA ATCCCAGGGG TTCAGACTCT 
GCTAATCTTG ATAGTGCTGC AGAAATCATG ATTAGAAGTG GAAGAACACC AGAGGAAGCT 
CTAATGATTC TTGTCCCTGA GGCATACAAG AATCATCCAA CCTTATCTGT CAAATATCCT 
GAGGTTGTAG ATTTCTATGA CTACTACAAA GGACAAATGG AGGCTTGGGA TGGTCCTGCC 
TTACTTTTGT TCAGTGATGG AAAAACAGTT GGGGCTTGTC TTGACCGTAA TGGCCTTCGT 
CCTGCTCGAT ATTGGCGGAC TAGTGACAAT TTCGTCTATG TTGCATCTGA GGTCGGAGTT 
GTACCAGTTG ATGAGGCAAA AGTCACAATG AAAGGCCGTC TAGGACCTGG AATGATGATT 
GCTGTTGACC TTGTGAATGG CCAGGTATAT GAGAATACAG AGGTCAAGAA GAGAATATCT 
TCATTTAATC CATATGGAAA ATGGATTAAA GAAAACTCCC GGTTCTTGAA GCCTGTGAAT 
TTCAAATCCT CAACTGTCAT GGAAAATGAA GAAATCCTAA GAAGCCAACA AGCATTTGGT 
TATTCAAGTG AGGATGTGCA AATGGTTATT GAGTCTATGG CTTCTCAAGG AAAGGAACCA 
ACCTTCTGCA TGGGCGACGA TATTCCGCTG GCAGGATTGT CTCAAAGACC GCATATGCTT 
TATGATTATT TCAAACAAAG ATTTGCACAG GTTACAAACC CTGCCATTGA CCCCCTTAGG 
GAAGGTTTGG TTATGTCTCT TGAAGTAAAT ATTGGAAAAC GTGGAAATAT ATTGGAGCTT 
GGACCTGAGA ATGCCTCGCA GGTTATTCTG TCTAACCCTG TGTTAAATGA AGGAGCGTTA 
GAGGAGTTAA TGAAGGATCA ATACTTAAAA CCAAAGGTTC TGTCCACATA TTTCGATATA 
AGAAAAGGCG TTGAAGGTTC CTTGCAAAAG GCTCTATATT ATCTTTGTGA AGCAGCTGAT 
GATGCTGTCC GAAGTGGCTC TCAGCTTCTC GTTCTTTCAG ACCGATCCGA TAGACTGGAA 
CCAACCAGGC CTTCAATTCC AATAATGTTA GCTGTTGGCG CTGTCCATCA ACATCTTATT 
CAGAACGGCT TGCGTATGTC AGCTTCTATT GTTGCTGATA CCGCCCAATG CTTCAGCACA 
CATCATTTTG CTTGTTTGGT TGGATATGGT GCAAGTGCTG TATGCCCATA CTTGGCACTG 
GAGACATGTA GGCAATGGCG CTTAAGTAAC AAAACTGTGG CCTTCATGCG TAACGGGAAA 
ATTCCTACTG TAACCATTGA GCAAGCTCAG AAGAACTACA CTAAGGCGGT TAATGCAGGG 
CTTCTTAAAA TTCTTTCTAA GATGGGAATC TCATTGCTTT CAAGTTATTG TGGTGCTCAG 
ATATTTGAGA TATATGGTTT GGGACAGGAT GTTGTTGATC TTGCATTCAC TGGAAGTGTG 
TCAAAAATCA GTGGACTCAC CTTTGATGAG TTGGCAAGAG AGACATTGTC TTTCTGGGTG 
AAGGCCTTTT CTGAGGATAC AACTAAGCGA TTAGAAAATT TTGGGTTTAT TCAATTCAGG 
CCTGGAGGTG AGTATCATTC AAACAACCCA GAGATGTCAA AGTTGCTTCA CAAGGCTGTC 
CGTGAAAAGA GTGAAACTGC ATATGCAGTC TATCAACAGC ATCTCTCTAA CAGACCTGTT 
AATGTCCTCC GTGACCTGCT TGAGTTCAAG AGTGATCGTG CACCGATCCC AGTAGGGAAA 
GTAGAACCGG CCGTTGCTAT TGTTCAGAGA TTTTGTACTG GTGGAATGTC ACTTGGTGCT 
ATTTCAAGAG AGACTCATGA AGCTATTGCT ATTGCAATGA ATAGGATTGG TGGGAAATCA 
AACTCTGGAG AAGGTGGAGA GGATCCTATC CGTTGGAAGC CACTTACAGA TGTGGTTGAT 
GGATATTCAC CAACACTACC ACATCTCAAA GGTCTTCAAA ACGGCGATAT TGCAACAAGT 
GCTATCAAGC AGGTTGCTTC AGGGCGTTTT GGAGTCACAC CAACGTTCTT GGTCAATGCA 
GATCAATTGG AAATCAAAGT TGCACAAGGT GCCAAGCCTG GGGAAGGTGG TCAGCTTCCT 
GGAAAGAAAG TTAGTGCGTA TATCGCTAGG CTAAGAAGCT CTAAACCTGG TGTTCCGCTT 
ATATCTCCGC CTCCTCACCA CGACATTTAC TCTATTGAGG ATCTTGCTCA GTTGATCTTT 
GATCTACATC AGATTAATCC AAATGCAAAA GTATCAGTCA AGCTAGTTGC AGAAGCTGGA 
ATCGGAACTG TTGCTTCAGG AGTTGCAAAG GGTAACGCTG ATATCATCCA GATATCAGGC 
CATGATGGTG GAACCGGGGC TAGTCCAATA AGCTCCATAA AACATGCTGG TGGACCATGG 
GAACTTGGAC TAACAGAAAC TCACCAAACA CTTATCGCAA ATGGACTAAG AGAAAGAGTC 
ATTTTAAGAG TCGATGGAGG CTTAAAGAGT GGTGTTGATG TTCTAATGGC TGCAGCTATG 
GGTGCTGATG AATACGGATT CGGTTCCTTG GCAATGATTG CTACTGGTTG TGTTATGGCT 
CGTATTTGCC ACACTAATAA TTGCCCAGTG GGTGTAGCAA GTCAGAGAGA AGAATTACGT 
GCAAGATTCC CTGGTGTACC TGGTGATCTT GTCAACTACT TCTTATACGT AGCAGAAGAG 
GTGAGAGGTA TCTTAGCACA GTTGGGATAC AACAGTTTAG ATGACATCAT TGGACGAACA 
GAGTTACTGA GACCACGAGA CATTTCGCTA GTTAAAACTC AACATCTCGA TCTGAGTTAT 
CTTCTTTCGT CTGTTGGAAC ACCTTCATTG AGCAGTACTG AAATCAGAAA GCAGGAAGTT 
CATACAAATG GACCTGTTCT CGACGACGAT ATTCTTGCAG ATCCATTGGT GATTGATGCA 
ATAGAGAACG AAAAAGTGGT TGAGAAAACC GTCAAAATAT GCAACGTAGA CCGTGCGGCT 
TGTGGTCGTG TTGCTGGTGT TATTGCAAAG AAGTATGGAG ACACTGGTTT TGCAGGACAA 
GTGAACCTAA CTTTCTTAGG GAGCGCGGGA CAGTCGTTTG GGTGCTTTTT GATTCCCGGT 
ATGAACATCC GGCTCATAGG AGAGTCAAAT GACTACGTTG GAAAGGGAAT GGCTGGTGGT 
GAAATAGTAG TAACTCCTGT GGAAAAAATC GGGTTTGTGC CTGAGGAAGC AACGATAGTC 
GGGAACACTT GCTTGTATGG TGCAACAGGT GGTCAGATAT TCGCTAGAGG CAAAGCTGGA 
GAGAGATTTG CAGTGAGAAA CTCACTCGCT GAAGCAGTAG TTGAAGGCAC TGGAGACCAT 
TGCTGTGAGT ACATGACTGG TGGCTGTGTA GTCGTGCTTG GAAAGGTGGG AAGAAACGTT 
GCTGCTGGTA TGACAGGAGG GTTAGCTTAC CTTCTTGATG AAGACGACAC TCTTCTTCCT 
AAGATTAACA GAGAGATAGT GAAGATCCAA AGAGTAACTG CGCCTGCAGG GGAATTGCAG 
CTGAAGAGCT TAATTGAAGC ACATGTGGAA AAAACCGGAA GCAGCAAAGG CGCAACGATT 
CTGAATGAGT GGGAAAAGTA TCTACCTCTC TTCTGGCAAC TGGTTCCACC GAGTGAGGAA 
GACACTCCTG AAGCTTCTGC TGCTTACGTA AGAACATCCA CCGGGGAAGT CACATTTCAA 
TCGGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLU1 ( NM_180432.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_850763.1 Arabidopsis thaliana glutamate synthase 1 (GLU1), partial mRNA.

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CloneID	OAb32130
Clone ID Related Accession (Same CDS sequence)	NM_180432.2
Accession Version	NM_180432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	glutamate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Feb 17, 2004 this sequence version replaced NM_180432.1. COMPLETENESS: incomplete on the 5' end.

ATGGCGATGC AATCTCTTTC CCCTGTTCCT AAGCTTCTCT CCACAACACC AAGCTCTGTT 
CTTTCTTCTG ACAAGAACTT CTTCTTCGTC GATTTCGTTG GATTATACTG TAAGTCCAAG 
AGGACCAGAC GCAGACTTCG TGGAGACTCT TCCTCTTCCT CACGCTCTTC TTCTTCTCTC 
TCTCGTCTTT CCTCTGTTCG TGCCGTTATC GACCTTGAAC GTGTTCATGG CGTCTCTGAA 
AAGGATCTCT CCTCTCCTTC AGCTTTAAGA CCTCAGGTTC GTTTCTTCAC TGATATAAAT 
TTCACAAACA CCCAACGTGC AAAGTTTCAT CCTTTATGGG GATCTTTTAA GCAGGTTGCT 
AATTTGGAGG ATATATTGTC TGAAAGAGGA GCTTGTGGAG TTGGGTTTAT AGCAAACTTA 
GATAACATAC CTTCACATGG AGTTGTCAAA GATGCTCTTA TTGCTCTTGG GTGTATGGAA 
CATCGTGGAG GTTGTGGAGC AGACAATGAT TCTGGTGATG GCTCTGGTCT TATGTCTTCC 
ATTCCTTGGG ATTTCTTTAA CGTCTGGGCC AAGGAACAAA GTCTTGCTCC TTTTGATAAG 
TTGCATACTG GTGTTGGCAT GATCTTTCTT CCACAAGATG ATACCTTTAT GCAAGAAGCC 
AAGCAAGTTA TTGAAAACAT ATTTGAGAAA GAAGGATTAC AAGTTCTTGG GTGGAGGGAA 
GTTCCTGTTA ATGTTCCTAT AGTTGGTAAA AATGCTAGGG AGACAATGCC TAACATTCAA 
CAAGTGTTTG TGAAAATCGC AAAGGAAGAT AGTACTGATG ATATTGAAAG GGAACTTTAC 
ATCTGCCGGA AACTAATCGA AAGGGCCGTA GCTACTGAGA GTTGGGGAAC AGAGCTTTAC 
TTCTGTTCAC TGTCCAATCA AACCATAGTG TACAAGGGCA TGCTTCGATC TGAAGCTCTT 
GGATTGTTTT ATCTAGATCT TCAGAATGAG CTTTATGAGT CTCCTTTTGC TATTTATCAT 
CGAAGGTACA GTACAAACAC TAGTCCTAGG TGGCCTCTTG CTCAACCAAT GAGGTTTCTT 
GGACATAACG GGGAGATCAA TACCATTCAG GGGAACTTAA ATTGGATGCA GTCTCGAGAA 
GCTTCATTGA AGGCTGCTGT TTGGAATGGC CGTGAAAATG AAATTCGTCC ATTTGGTAAT 
CCCAGGGGTT CAGACTCTGC TAATCTTGAT AGTGCTGCAG AAATCATGAT TAGAAGTGGA 
AGAACACCAG AGGAAGCTCT AATGATTCTT GTCCCTGAGG CATACAAGAA TCATCCAACC 
TTATCTGTCA AATATCCTGA GGTTGTAGAT TTCTATGACT ACTACAAAGG ACAAATGGAG 
GCTTGGGATG GTCCTGCCTT ACTTTTGTTC AGTGATGGAA AAACAGTTGG GGCTTGTCTT 
GACCGTAATG GCCTTCGTCC TGCTCGATAT TGGCGGACTA GTGACAATTT CGTCTATGTT 
GCATCTGAGG TCGGAGTTGT ACCAGTTGAT GAGGCAAAAG TCACAATGAA AGGCCGTCTA 
GGACCTGGAA TGATGATTGC TGTTGACCTT GTGAATGGCC AGGTATATGA GAATACAGAG 
GTCAAGAAGA GAATATCTTC ATTTAATCCA TATGGAAAAT GGATTAAAGA AAACTCCCGG 
TTCTTGAAGC CTGTGAATTT CAAATCCTCA ACTGTCATGG AAAATGAAGA AATCCTAAGA 
AGCCAACAAG CATTTGGTTA TTCAAGTGAG GATGTGCAAA TGGTTATTGA GTCTATGGCT 
TCTCAAGGAA AGGAACCAAC CTTCTGCATG GGCGACGATA TTCCGCTGGC AGGATTGTCT 
CAAAGACCGC ATATGCTTTA TGATTATTTC AAACAAAGAT TTGCACAGGT TACAAACCCT 
GCCATTGACC CCCTTAGGGA AGGTTTGGTT ATGTCTCTTG AAGTAAATAT TGGAAAACGT 
GGAAATATAT TGGAGCTTGG ACCTGAGAAT GCCTCGCAGG TTATTCTGTC TAACCCTGTG 
TTAAATGAAG GAGCGTTAGA GGAGTTAATG AAGGATCAAT ACTTAAAACC AAAGGTTCTG 
TCCACATATT TCGATATAAG AAAAGGCGTT GAAGGTTCCT TGCAAAAGGC TCTATATTAT 
CTTTGTGAAG CAGCTGATGA TGCTGTCCGA AGTGGCTCTC AGCTTCTCGT TCTTTCAGAC 
CGATCCGATA GACTGGAACC AACCAGGCCT TCAATTCCAA TAATGTTAGC TGTTGGCGCT 
GTCCATCAAC ATCTTATTCA GAACGGCTTG CGTATGTCAG CTTCTATTGT TGCTGATACC 
GCCCAATGCT TCAGCACACA TCATTTTGCT TGTTTGGTTG GATATGGTGC AAGTGCTGTA 
TGCCCATACT TGGCACTGGA GACATGTAGG CAATGGCGCT TAAGTAACAA AACTGTGGCC 
TTCATGCGTA ACGGGAAAAT TCCTACTGTA ACCATTGAGC AAGCTCAGAA GAACTACACT 
AAGGCGGTTA ATGCAGGGCT TCTTAAAATT CTTTCTAAGA TGGGAATCTC ATTGCTTTCA 
AGTTATTGTG GTGCTCAGAT ATTTGAGATA TATGGTTTGG GACAGGATGT TGTTGATCTT 
GCATTCACTG GAAGTGTGTC AAAAATCAGT GGACTCACCT TTGATGAGTT GGCAAGAGAG 
ACATTGTCTT TCTGGGTGAA GGCCTTTTCT GAGGATACAA CTAAGCGATT AGAAAATTTT 
GGGTTTATTC AATTCAGGCC TGGAGGTGAG TATCATTCAA ACAACCCAGA GATGTCAAAG 
TTGCTTCACA AGGCTGTCCG TGAAAAGAGT GAAACTGCAT ATGCAGTCTA TCAACAGCAT 
CTCTCTAACA GACCTGTTAA TGTCCTCCGT GACCTGCTTG AGTTCAAGAG TGATCGTGCA 
CCGATCCCAG TAGGGAAAGT AGAACCGGCC GTTGCTATTG TTCAGAGATT TTGTACTGGT 
GGAATGTCAC TTGGTGCTAT TTCAAGAGAG ACTCATGAAG CTATTGCTAT TGCAATGAAT 
AGGATTGGTG GGAAATCAAA CTCTGGAGAA GGTGGAGAGG ATCCTATCCG TTGGAAGCCA 
CTTACAGATG TGGTTGATGG ATATTCACCA ACACTACCAC ATCTCAAAGG TCTTCAAAAC 
GGCGATATTG CAACAAGTGC TATCAAGCAG GTTGCTTCAG GGCGTTTTGG AGTCACACCA 
ACGTTCTTGG TCAATGCAGA TCAATTGGAA ATCAAAGTTG CACAAGGTGC CAAGCCTGGG 
GAAGGTGGTC AGCTTCCTGG AAAGAAAGTT AGTGCGTATA TCGCTAGGCT AAGAAGCTCT 
AAACCTGGTG TTCCGCTTAT ATCTCCGCCT CCTCACCACG ACATTTACTC TATTGAGGAT 
CTTGCTCAGT TGATCTTTGA TCTACATCAG ATTAATCCAA ATGCAAAAGT ATCAGTCAAG 
CTAGTTGCAG AAGCTGGAAT CGGAACTGTT GCTTCAGGAG TTGCAAAGGG TAACGCTGAT 
ATCATCCAGA TATCAGGCCA TGATGGTGGA ACCGGGGCTA GTCCAATAAG CTCCATAAAA 
CATGCTGGTG GACCATGGGA ACTTGGACTA ACAGAAACTC ACCAAACACT TATCGCAAAT 
GGACTAAGAG AAAGAGTCAT TTTAAGAGTC GATGGAGGCT TAAAGAGTGG TGTTGATGTT 
CTAATGGCTG CAGCTATGGG TGCTGATGAA TACGGATTCG GTTCCTTGGC AATGATTGCT 
ACTGGTTGTG TTATGGCTCG TATTTGCCAC ACTAATAATT GCCCAGTGGG TGTAGCAAGT 
CAGAGAGAAG AATTACGTGC AAGATTCCCT GGTGTACCTG GTGATCTTGT CAACTACTTC 
TTATACGTAG CAGAAGAGGT GAGAGGTATC TTAGCACAGT TGGGATACAA CAGTTTAGAT 
GACATCATTG GACGAACAGA GTTACTGAGA CCACGAGACA TTTCGCTAGT TAAAACTCAA 
CATCTCGATC TGAGTTATCT TCTTTCGTCT GTTGGAACAC CTTCATTGAG CAGTACTGAA 
ATCAGAAAGC AGGAAGTTCA TACAAATGGA CCTGTTCTCG ACGACGATAT TCTTGCAGAT 
CCATTGGTGA TTGATGCAAT AGAGAACGAA AAAGTGGTTG AGAAAACCGT CAAAATATGC 
AACGTAGACC GTGCGGCTTG TGGTCGTGTT GCTGGTGTTA TTGCAAAGAA GTATGGAGAC 
ACTGGTTTTG CAGGACAAGT GAACCTAACT TTCTTAGGGA GCGCGGGACA GTCGTTTGGG 
TGCTTTTTGA TTCCCGGTAT GAACATCCGG CTCATAGGAG AGTCAAATGA CTACGTTGGA 
AAGGGAATGG CTGGTGGTGA AATAGTAGTA ACTCCTGTGG AAAAAATCGG GTTTGTGCCT 
GAGGAAGCAA CGATAGTCGG GAACACTTGC TTGTATGGTG CAACAGGTGG TCAGATATTC 
GCTAGAGGCA AAGCTGGAGA GAGATTTGCA GTGAGAAACT CACTCGCTGA AGCAGTAGTT 
GAAGGCACTG GAGACCATTG CTGTGAGTAC ATGACTGGTG GCTGTGTAGT CGTGCTTGGA 
AAGGTGGGAA GAAACGTTGC TGCTGGTATG ACAGGAGGGT TAGCTTACCT TCTTGATGAA 
GACGACACTC TTCTTCCTAA GATTAACAGA GAGATAGTGA AGATCCAAAG AGTAACTGCG 
CCTGCAGGGG AATTGCAGCT GAAGAGCTTA ATTGAAGCAC ATGTGGAAAA AACCGGAAGC 
AGCAAAGGCG CAACGATTCT GAATGAGTGG GAAAAGTATC TACCTCTCTT CTGGCAACTG 
GTTCCACCGA GTGAGGAAGA CACTCCTGAA GCTTCTGCTG CTTACGTAAG AACATCCACC 
GGGGAAGTCA CATTTCAATC GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_850763.1
CDS	1..4947
Translation	MAMQSLSPVPKLLSTTPSSVLSSDKNFFFVDFVGLYCKSKRTRRRLRGDSSSSSRSSSSLSRLSSVRAVIDLERVHGVSEKDLSSPSALRPQVRFFTDINFTNTQRAKFHPLWGSFKQVANLEDILSERGACGVGFIANLDNIPSHGVVKDALIALGCMEHRGGCGADNDSGDGSGLMSSIPWDFFNVWAKEQSLAPFDKLHTGVGMIFLPQDDTFMQEAKQVIENIFEKEGLQVLGWREVPVNVPIVGKNARETMPNIQQVFVKIAKEDSTDDIERELYICRKLIERAVATESWGTELYFCSLSNQTIVYKGMLRSEALGLFYLDLQNELYESPFAIYHRRYSTNTSPRWPLAQPMRFLGHNGEINTIQGNLNWMQSREASLKAAVWNGRENEIRPFGNPRGSDSANLDSAAEIMIRSGRTPEEALMILVPEAYKNHPTLSVKYPEVVDFYDYYKGQMEAWDGPALLLFSDGKTVGACLDRNGLRPARYWRTSDNFVYVASEVGVVPVDEAKVTMKGRLGPGMMIAVDLVNGQVYENTEVKKRISSFNPYGKWIKENSRFLKPVNFKSSTVMENEEILRSQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAGLSQRPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILELGPENASQVILSNPVLNEGALEELMKDQYLKPKVLSTYFDIRKGVEGSLQKALYYLCEAADDAVRSGSQLLVLSDRSDRLEPTRPSIPIMLAVGAVHQHLIQNGLRMSASIVADTAQCFSTHHFACLVGYGASAVCPYLALETCRQWRLSNKTVAFMRNGKIPTVTIEQAQKNYTKAVNAGLLKILSKMGISLLSSYCGAQIFEIYGLGQDVVDLAFTGSVSKISGLTFDELARETLSFWVKAFSEDTTKRLENFGFIQFRPGGEYHSNNPEMSKLLHKAVREKSETAYAVYQQHLSNRPVNVLRDLLEFKSDRAPIPVGKVEPAVAIVQRFCTGGMSLGAISRETHEAIAIAMNRIGGKSNSGEGGEDPIRWKPLTDVVDGYSPTLPHLKGLQNGDIATSAIKQVASGRFGVTPTFLVNADQLEIKVAQGAKPGEGGQLPGKKVSAYIARLRSSKPGVPLISPPPHHDIYSIEDLAQLIFDLHQINPNAKVSVKLVAEAGIGTVASGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIANGLRERVILRVDGGLKSGVDVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNYFLYVAEEVRGILAQLGYNSLDDIIGRTELLRPRDISLVKTQHLDLSYLLSSVGTPSLSSTEIRKQEVHTNGPVLDDDILADPLVIDAIENEKVVEKTVKICNVDRAACGRVAGVIAKKYGDTGFAGQVNLTFLGSAGQSFGCFLIPGMNIRLIGESNDYVGKGMAGGEIVVTPVEKIGFVPEEATIVGNTCLYGATGGQIFARGKAGERFAVRNSLAEAVVEGTGDHCCEYMTGGCVVVLGKVGRNVAAGMTGGLAYLLDEDDTLLPKINREIVKIQRVTAPAGELQLKSLIEAHVEKTGSSKGATILNEWEKYLPLFWQLVPPSEEDTPEASAAYVRTSTGEVTFQSA

Target ORF information:

RefSeq Version	NM_180432.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana glutamate synthase 1 (GLU1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180432.2

ORF Insert Sequence:

ATGGCGATGC AATCTCTTTC CCCTGTTCCT AAGCTTCTCT CCACAACACC AAGCTCTGTT 
CTTTCTTCTG ACAAGAACTT CTTCTTCGTC GATTTCGTTG GATTATACTG TAAGTCCAAG 
AGGACCAGAC GCAGACTTCG TGGAGACTCT TCCTCTTCCT CACGCTCTTC TTCTTCTCTC 
TCTCGTCTTT CCTCTGTTCG TGCCGTTATC GACCTTGAAC GTGTTCATGG CGTCTCTGAA 
AAGGATCTCT CCTCTCCTTC AGCTTTAAGA CCTCAGGTTC GTTTCTTCAC TGATATAAAT 
TTCACAAACA CCCAACGTGC AAAGTTTCAT CCTTTATGGG GATCTTTTAA GCAGGTTGCT 
AATTTGGAGG ATATATTGTC TGAAAGAGGA GCTTGTGGAG TTGGGTTTAT AGCAAACTTA 
GATAACATAC CTTCACATGG AGTTGTCAAA GATGCTCTTA TTGCTCTTGG GTGTATGGAA 
CATCGTGGAG GTTGTGGAGC AGACAATGAT TCTGGTGATG GCTCTGGTCT TATGTCTTCC 
ATTCCTTGGG ATTTCTTTAA CGTCTGGGCC AAGGAACAAA GTCTTGCTCC TTTTGATAAG 
TTGCATACTG GTGTTGGCAT GATCTTTCTT CCACAAGATG ATACCTTTAT GCAAGAAGCC 
AAGCAAGTTA TTGAAAACAT ATTTGAGAAA GAAGGATTAC AAGTTCTTGG GTGGAGGGAA 
GTTCCTGTTA ATGTTCCTAT AGTTGGTAAA AATGCTAGGG AGACAATGCC TAACATTCAA 
CAAGTGTTTG TGAAAATCGC AAAGGAAGAT AGTACTGATG ATATTGAAAG GGAACTTTAC 
ATCTGCCGGA AACTAATCGA AAGGGCCGTA GCTACTGAGA GTTGGGGAAC AGAGCTTTAC 
TTCTGTTCAC TGTCCAATCA AACCATAGTG TACAAGGGCA TGCTTCGATC TGAAGCTCTT 
GGATTGTTTT ATCTAGATCT TCAGAATGAG CTTTATGAGT CTCCTTTTGC TATTTATCAT 
CGAAGGTACA GTACAAACAC TAGTCCTAGG TGGCCTCTTG CTCAACCAAT GAGGTTTCTT 
GGACATAACG GGGAGATCAA TACCATTCAG GGGAACTTAA ATTGGATGCA GTCTCGAGAA 
GCTTCATTGA AGGCTGCTGT TTGGAATGGC CGTGAAAATG AAATTCGTCC ATTTGGTAAT 
CCCAGGGGTT CAGACTCTGC TAATCTTGAT AGTGCTGCAG AAATCATGAT TAGAAGTGGA 
AGAACACCAG AGGAAGCTCT AATGATTCTT GTCCCTGAGG CATACAAGAA TCATCCAACC 
TTATCTGTCA AATATCCTGA GGTTGTAGAT TTCTATGACT ACTACAAAGG ACAAATGGAG 
GCTTGGGATG GTCCTGCCTT ACTTTTGTTC AGTGATGGAA AAACAGTTGG GGCTTGTCTT 
GACCGTAATG GCCTTCGTCC TGCTCGATAT TGGCGGACTA GTGACAATTT CGTCTATGTT 
GCATCTGAGG TCGGAGTTGT ACCAGTTGAT GAGGCAAAAG TCACAATGAA AGGCCGTCTA 
GGACCTGGAA TGATGATTGC TGTTGACCTT GTGAATGGCC AGGTATATGA GAATACAGAG 
GTCAAGAAGA GAATATCTTC ATTTAATCCA TATGGAAAAT GGATTAAAGA AAACTCCCGG 
TTCTTGAAGC CTGTGAATTT CAAATCCTCA ACTGTCATGG AAAATGAAGA AATCCTAAGA 
AGCCAACAAG CATTTGGTTA TTCAAGTGAG GATGTGCAAA TGGTTATTGA GTCTATGGCT 
TCTCAAGGAA AGGAACCAAC CTTCTGCATG GGCGACGATA TTCCGCTGGC AGGATTGTCT 
CAAAGACCGC ATATGCTTTA TGATTATTTC AAACAAAGAT TTGCACAGGT TACAAACCCT 
GCCATTGACC CCCTTAGGGA AGGTTTGGTT ATGTCTCTTG AAGTAAATAT TGGAAAACGT 
GGAAATATAT TGGAGCTTGG ACCTGAGAAT GCCTCGCAGG TTATTCTGTC TAACCCTGTG 
TTAAATGAAG GAGCGTTAGA GGAGTTAATG AAGGATCAAT ACTTAAAACC AAAGGTTCTG 
TCCACATATT TCGATATAAG AAAAGGCGTT GAAGGTTCCT TGCAAAAGGC TCTATATTAT 
CTTTGTGAAG CAGCTGATGA TGCTGTCCGA AGTGGCTCTC AGCTTCTCGT TCTTTCAGAC 
CGATCCGATA GACTGGAACC AACCAGGCCT TCAATTCCAA TAATGTTAGC TGTTGGCGCT 
GTCCATCAAC ATCTTATTCA GAACGGCTTG CGTATGTCAG CTTCTATTGT TGCTGATACC 
GCCCAATGCT TCAGCACACA TCATTTTGCT TGTTTGGTTG GATATGGTGC AAGTGCTGTA 
TGCCCATACT TGGCACTGGA GACATGTAGG CAATGGCGCT TAAGTAACAA AACTGTGGCC 
TTCATGCGTA ACGGGAAAAT TCCTACTGTA ACCATTGAGC AAGCTCAGAA GAACTACACT 
AAGGCGGTTA ATGCAGGGCT TCTTAAAATT CTTTCTAAGA TGGGAATCTC ATTGCTTTCA 
AGTTATTGTG GTGCTCAGAT ATTTGAGATA TATGGTTTGG GACAGGATGT TGTTGATCTT 
GCATTCACTG GAAGTGTGTC AAAAATCAGT GGACTCACCT TTGATGAGTT GGCAAGAGAG 
ACATTGTCTT TCTGGGTGAA GGCCTTTTCT GAGGATACAA CTAAGCGATT AGAAAATTTT 
GGGTTTATTC AATTCAGGCC TGGAGGTGAG TATCATTCAA ACAACCCAGA GATGTCAAAG 
TTGCTTCACA AGGCTGTCCG TGAAAAGAGT GAAACTGCAT ATGCAGTCTA TCAACAGCAT 
CTCTCTAACA GACCTGTTAA TGTCCTCCGT GACCTGCTTG AGTTCAAGAG TGATCGTGCA 
CCGATCCCAG TAGGGAAAGT AGAACCGGCC GTTGCTATTG TTCAGAGATT TTGTACTGGT 
GGAATGTCAC TTGGTGCTAT TTCAAGAGAG ACTCATGAAG CTATTGCTAT TGCAATGAAT 
AGGATTGGTG GGAAATCAAA CTCTGGAGAA GGTGGAGAGG ATCCTATCCG TTGGAAGCCA 
CTTACAGATG TGGTTGATGG ATATTCACCA ACACTACCAC ATCTCAAAGG TCTTCAAAAC 
GGCGATATTG CAACAAGTGC TATCAAGCAG GTTGCTTCAG GGCGTTTTGG AGTCACACCA 
ACGTTCTTGG TCAATGCAGA TCAATTGGAA ATCAAAGTTG CACAAGGTGC CAAGCCTGGG 
GAAGGTGGTC AGCTTCCTGG AAAGAAAGTT AGTGCGTATA TCGCTAGGCT AAGAAGCTCT 
AAACCTGGTG TTCCGCTTAT ATCTCCGCCT CCTCACCACG ACATTTACTC TATTGAGGAT 
CTTGCTCAGT TGATCTTTGA TCTACATCAG ATTAATCCAA ATGCAAAAGT ATCAGTCAAG 
CTAGTTGCAG AAGCTGGAAT CGGAACTGTT GCTTCAGGAG TTGCAAAGGG TAACGCTGAT 
ATCATCCAGA TATCAGGCCA TGATGGTGGA ACCGGGGCTA GTCCAATAAG CTCCATAAAA 
CATGCTGGTG GACCATGGGA ACTTGGACTA ACAGAAACTC ACCAAACACT TATCGCAAAT 
GGACTAAGAG AAAGAGTCAT TTTAAGAGTC GATGGAGGCT TAAAGAGTGG TGTTGATGTT 
CTAATGGCTG CAGCTATGGG TGCTGATGAA TACGGATTCG GTTCCTTGGC AATGATTGCT 
ACTGGTTGTG TTATGGCTCG TATTTGCCAC ACTAATAATT GCCCAGTGGG TGTAGCAAGT 
CAGAGAGAAG AATTACGTGC AAGATTCCCT GGTGTACCTG GTGATCTTGT CAACTACTTC 
TTATACGTAG CAGAAGAGGT GAGAGGTATC TTAGCACAGT TGGGATACAA CAGTTTAGAT 
GACATCATTG GACGAACAGA GTTACTGAGA CCACGAGACA TTTCGCTAGT TAAAACTCAA 
CATCTCGATC TGAGTTATCT TCTTTCGTCT GTTGGAACAC CTTCATTGAG CAGTACTGAA 
ATCAGAAAGC AGGAAGTTCA TACAAATGGA CCTGTTCTCG ACGACGATAT TCTTGCAGAT 
CCATTGGTGA TTGATGCAAT AGAGAACGAA AAAGTGGTTG AGAAAACCGT CAAAATATGC 
AACGTAGACC GTGCGGCTTG TGGTCGTGTT GCTGGTGTTA TTGCAAAGAA GTATGGAGAC 
ACTGGTTTTG CAGGACAAGT GAACCTAACT TTCTTAGGGA GCGCGGGACA GTCGTTTGGG 
TGCTTTTTGA TTCCCGGTAT GAACATCCGG CTCATAGGAG AGTCAAATGA CTACGTTGGA 
AAGGGAATGG CTGGTGGTGA AATAGTAGTA ACTCCTGTGG AAAAAATCGG GTTTGTGCCT 
GAGGAAGCAA CGATAGTCGG GAACACTTGC TTGTATGGTG CAACAGGTGG TCAGATATTC 
GCTAGAGGCA AAGCTGGAGA GAGATTTGCA GTGAGAAACT CACTCGCTGA AGCAGTAGTT 
GAAGGCACTG GAGACCATTG CTGTGAGTAC ATGACTGGTG GCTGTGTAGT CGTGCTTGGA 
AAGGTGGGAA GAAACGTTGC TGCTGGTATG ACAGGAGGGT TAGCTTACCT TCTTGATGAA 
GACGACACTC TTCTTCCTAA GATTAACAGA GAGATAGTGA AGATCCAAAG AGTAACTGCG 
CCTGCAGGGG AATTGCAGCT GAAGAGCTTA ATTGAAGCAC ATGTGGAAAA AACCGGAAGC 
AGCAAAGGCG CAACGATTCT GAATGAGTGG GAAAAGTATC TACCTCTCTT CTGGCAACTG 
GTTCCACCGA GTGAGGAAGA CACTCCTGAA GCTTCTGCTG CTTACGTAAG AACATCCACC 
GGGGAAGTCA CATTTCAATC GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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GLU1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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