MUS81 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MUS81
Entrez Gene ID	829211
Full Name	Restriction endonuclease, type II-like superfamily protein
Synonyms	ARABIDOPSIS THALIANA MMS AND UV SENSITIVE 81,ATMUS81,F6I18.220,F6I18_220,MMS AND UV SENSITIVE 81
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119234.4	NP_194816.2	Restriction endonuclease, type II-like superfamily protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	MUS81	NP_194816.2
Arabidopsis thaliana (thale cress)	AT5G39770	NP_198793.4
Oryza sativa (rice)	Os01g0948100	NP_001045390.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SEND1 has at most a minor role in resolution of the Holliday junction but acts as an essential backup to MUS81 for resolution of toxic replication structures to ensure genome stability and to maintain telomere integrity.
Title: The Structure-Specific Endonucleases MUS81 and SEND1 Are Essential for Telomere Stability in Arabidopsis.

Data show that DNA polymerase zeta catalytic subunit REV3 cooperates with ATP-dependent DNA helicase RECQ4A and e ndonuclease MUS81 to repair DNA interstrand cross links.
Title: The Translesion Polymerase ? Has Roles Dependent on and Independent of the Nuclease MUS81 and the Helicase RECQ4A in DNA Damage Repair in Arabidopsis.

RECQ4A and MUS81 define RAD5A-independent pathways of DNA repair. RECQ4A and MUS81 act in parallel pathways presumably to resolve replication-associated DNA intermediates.
Title: RAD5A, RECQ4A, and MUS81 have specific functions in homologous recombination and define different pathways of DNA repair in Arabidopsis thaliana.

Both MUS81-EME1 endonuclease complexes are involved in DNA recombination and repair processes in Arabidopsis.
Title: Two distinct MUS81-EME1 complexes from Arabidopsis process Holliday junctions.

The role of MUS81 in the reproduction and growth of A. thaliana, via its expression and metabolism during meiosis, is reported.
Title: Expression and functional analysis of AtMUS81 in Arabidopsis meiosis reveals a role in the second pathway of crossing-over.

The following MUS81 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MUS81 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16795	NM_119234.4 Latest version!	Arabidopsis thaliana Restriction endonuclease, type II-like superfamily protein (MUS81), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MUS81 ( NM_119234.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194816.2 Arabidopsis thaliana Restriction endonuclease, type II-like superfamily protein (MUS81), mRNA.

Price & Availability↑

CloneID	OAb16795
Clone ID Related Accession (Same CDS sequence)	NM_119234.4
Accession Version	NM_119234.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Restriction endonuclease, type II-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119234.3.

ATGGATGACG AGAGACGGGT ACTGTGCCCT GAGAACCGAG GTTTAGCGGC TTATGTGTTG 
CAGAAGAAGC AAGAATACGC AGAAAAGCCC AAAGGCTTGT CGGAAAATCT GGAGAGGACA 
TTTGTTAAGG GGTATAGGAG TGTCTGCGAC GCCAAGGATC CCATTAACAC ACTCAAAGAC 
TTGTCGCAGA TCAAGGGCTT TGGCAAGTGG ATGGTTAAGC TCATGAAAGG ATATTTTGAT 
ACTGCTGTTG AAAGTTCTGA GCAAGAAGAT TTACCTGATA ATCGTGCTGG GAAAAAGGCC 
AATGGAAAAA AACGGTACAT TCCGCAAAGA AATTCAGTGG GATATGCATT ACTAATAACG 
TTGCACAGAA GGACTACAAA TGGGAAAGAA TTCATGCGTA AACAAGAGCT TATTGATGCT 
GCTGACGCGA ATGGGCTATC ACACGCTCCT GTTGGGCCAG AGAAAGGGAA AGGGAAAGCA 
GGACTTGGAC ACTCTAAGAG GGAGTGGTAT AGTGGATGGA GCTGCATGAC CACTCTTATA 
CAGAAGGGAT TAGTGGTGAA ATCTAGCAAC CCAGCAAAGT ACATGCTGAC AGTTGAAGGT 
CGGGAAGTTG CAAATGAGTG TATCCTGCGA TCTGGATTAC CTGATTCAGT TGATATCTTA 
TCTGTTGATG AAATGGATCC TACACCGCAA GCAAAGAAAA CACCTAATCA AAATCCCACC 
TGTTCGTTTA CCATGAGAGA AGAACTTCCA TATGTCGATC CAAGATGTAG GGCACAATCA 
GCCATTCCAA GCGATATTCT TGAAAAGTTT ACTCCGTTTG GTTATTCGAA GGAACAAGTC 
GTTGCTGCAT TCAGAGAGGT TTCAGATGGA TCTGGGGACA AAGATCCATC AACTCTCTGG 
CTCTCTGTAA TGTGCCATCT TCGTCAAGCA GAGGTTTATA ATTCCTGTCC GGACTCGAGA 
AATAGTAAAA AGGATTCATC AGGACCTTTT AAATCACAAA TTCGTCAAGT TGATCTTGAG 
GGATCTCGTG CTAAGAAGTT CCGCTCTTGC AATGATGGAT CCACCTTAAA TCCATGTTCA 
TCAGGTTCAA GTCATGCTGT GAAAGCCTGC TCATCTTCTT TGGCATCAGA TGGAACCAAA 
GGAATAACAA ATATTCCACG CCTCCCTCCT CTTCAATTTG GAGAAACGTT TGAGGAAGCA 
TATGATGTGA TCTTGATACT GGATGATCGA GAAAAGTTTG CCACCAAAGG ATCACGATCC 
AGGAATATAG TTGAAAACAT ATGCTCTGAA TTCAACATCA AGATTGAGGT TAGACGATTA 
CCAGTCGGGG ATTGTATATG GATTGCTCGG CATAAGTACC TTGAGACTGA ATATGTTCTA 
GACTTTATTG CTGAGAGGAA GAACGTTGAT GATATGCGCT CATCAATTAG AGATAATCGC 
TACAGAGACC AAAAACTTAG ACTTCAGAGA TCGGGATTCA AGAAGCTCAT ATACATTCTT 
GAAGGGGATC CAAACCATTC TGATGCAGCA GAAAGCATTA AAACAGCTTG TTTCACGACA 
GAGATTCTGG AGGGATTTGA TGTGCTGAGG ACACACGGGC TGGGTGAGAC GCTAAGAAAA 
TACGGCTACC TCACGAAATC AATATATCAA TACTACAAGT TACGGGTGAA TGATAATGAC 
CAGAGCAAAG GCGCAGCCTC ATGTCCTTCT TTTGATAGTT TTGTCAAAAG GTGTCAAGAC 
CTTGATAAAA TGACAATCAG CGATGTATTT GCCATTCAGC TAATGCAGGT CCCGCAGGTA 
ACGGAGGAAA TTGCCATAGC GGTTCTTGAT ATGTACCCAA CGCTTTTGTC TCTTGCTTCT 
GCCTATTCTC ATCTAGAAGC AGATGTCTCG GCGCAAGAAG AGATGTTGAG AAATAGAAGC 
AACAATGTAA TATGTGCATC AGCTAGTAAG AATATATTCA AGTTAGTTTG GGGTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194816.2
CDS	207..2186
Translation	MDDERRVLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLKDLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLITLHRRTTNGKEFMRKQELIDAADANGLSHAPVGPEKGKGKAGLGHSKREWYSGWSCMTTLIQKGLVVKSSNPAKYMLTVEGREVANECILRSGLPDSVDILSVDEMDPTPQAKKTPNQNPTCSFTMREELPYVDPRCRAQSAIPSDILEKFTPFGYSKEQVVAAFREVSDGSGDKDPSTLWLSVMCHLRQAEVYNSCPDSRNSKKDSSGPFKSQIRQVDLEGSRAKKFRSCNDGSTLNPCSSGSSHAVKACSSSLASDGTKGITNIPRLPPLQFGETFEEAYDVILILDDREKFATKGSRSRNIVENICSEFNIKIEVRRLPVGDCIWIARHKYLETEYVLDFIAERKNVDDMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDPNHSDAAESIKTACFTTEILEGFDVLRTHGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKGAASCPSFDSFVKRCQDLDKMTISDVFAIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSHLEADVSAQEEMLRNRSNNVICASASKNIFKLVWGE

Target ORF information:

RefSeq Version	NM_119234.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Restriction endonuclease, type II-like superfamily protein (MUS81), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119234.4

ORF Insert Sequence:

ATGGATGACG AGAGACGGGT ACTGTGCCCT GAGAACCGAG GTTTAGCGGC TTATGTGTTG 
CAGAAGAAGC AAGAATACGC AGAAAAGCCC AAAGGCTTGT CGGAAAATCT GGAGAGGACA 
TTTGTTAAGG GGTATAGGAG TGTCTGCGAC GCCAAGGATC CCATTAACAC ACTCAAAGAC 
TTGTCGCAGA TCAAGGGCTT TGGCAAGTGG ATGGTTAAGC TCATGAAAGG ATATTTTGAT 
ACTGCTGTTG AAAGTTCTGA GCAAGAAGAT TTACCTGATA ATCGTGCTGG GAAAAAGGCC 
AATGGAAAAA AACGGTACAT TCCGCAAAGA AATTCAGTGG GATATGCATT ACTAATAACG 
TTGCACAGAA GGACTACAAA TGGGAAAGAA TTCATGCGTA AACAAGAGCT TATTGATGCT 
GCTGACGCGA ATGGGCTATC ACACGCTCCT GTTGGGCCAG AGAAAGGGAA AGGGAAAGCA 
GGACTTGGAC ACTCTAAGAG GGAGTGGTAT AGTGGATGGA GCTGCATGAC CACTCTTATA 
CAGAAGGGAT TAGTGGTGAA ATCTAGCAAC CCAGCAAAGT ACATGCTGAC AGTTGAAGGT 
CGGGAAGTTG CAAATGAGTG TATCCTGCGA TCTGGATTAC CTGATTCAGT TGATATCTTA 
TCTGTTGATG AAATGGATCC TACACCGCAA GCAAAGAAAA CACCTAATCA AAATCCCACC 
TGTTCGTTTA CCATGAGAGA AGAACTTCCA TATGTCGATC CAAGATGTAG GGCACAATCA 
GCCATTCCAA GCGATATTCT TGAAAAGTTT ACTCCGTTTG GTTATTCGAA GGAACAAGTC 
GTTGCTGCAT TCAGAGAGGT TTCAGATGGA TCTGGGGACA AAGATCCATC AACTCTCTGG 
CTCTCTGTAA TGTGCCATCT TCGTCAAGCA GAGGTTTATA ATTCCTGTCC GGACTCGAGA 
AATAGTAAAA AGGATTCATC AGGACCTTTT AAATCACAAA TTCGTCAAGT TGATCTTGAG 
GGATCTCGTG CTAAGAAGTT CCGCTCTTGC AATGATGGAT CCACCTTAAA TCCATGTTCA 
TCAGGTTCAA GTCATGCTGT GAAAGCCTGC TCATCTTCTT TGGCATCAGA TGGAACCAAA 
GGAATAACAA ATATTCCACG CCTCCCTCCT CTTCAATTTG GAGAAACGTT TGAGGAAGCA 
TATGATGTGA TCTTGATACT GGATGATCGA GAAAAGTTTG CCACCAAAGG ATCACGATCC 
AGGAATATAG TTGAAAACAT ATGCTCTGAA TTCAACATCA AGATTGAGGT TAGACGATTA 
CCAGTCGGGG ATTGTATATG GATTGCTCGG CATAAGTACC TTGAGACTGA ATATGTTCTA 
GACTTTATTG CTGAGAGGAA GAACGTTGAT GATATGCGCT CATCAATTAG AGATAATCGC 
TACAGAGACC AAAAACTTAG ACTTCAGAGA TCGGGATTCA AGAAGCTCAT ATACATTCTT 
GAAGGGGATC CAAACCATTC TGATGCAGCA GAAAGCATTA AAACAGCTTG TTTCACGACA 
GAGATTCTGG AGGGATTTGA TGTGCTGAGG ACACACGGGC TGGGTGAGAC GCTAAGAAAA 
TACGGCTACC TCACGAAATC AATATATCAA TACTACAAGT TACGGGTGAA TGATAATGAC 
CAGAGCAAAG GCGCAGCCTC ATGTCCTTCT TTTGATAGTT TTGTCAAAAG GTGTCAAGAC 
CTTGATAAAA TGACAATCAG CGATGTATTT GCCATTCAGC TAATGCAGGT CCCGCAGGTA 
ACGGAGGAAA TTGCCATAGC GGTTCTTGAT ATGTACCCAA CGCTTTTGTC TCTTGCTTCT 
GCCTATTCTC ATCTAGAAGC AGATGTCTCG GCGCAAGAAG AGATGTTGAG AAATAGAAGC 
AACAATGTAA TATGTGCATC AGCTAGTAAG AATATATTCA AGTTAGTTTG GGGTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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MUS81 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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