AT4G27510 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G27510
Entrez Gene ID	828860
Full Name	2-isopropylmalate synthase
Synonyms	F27G19.110,F27G19_110
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001125598.1	NP_001119070.1	2-isopropylmalate synthase
NM_118886.4	NP_194481.2	2-isopropylmalate synthase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT4G27510 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G27510 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb01948	NM_001125598.1 Latest version!	Arabidopsis thaliana 2-isopropylmalate synthase (AT4G27510), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb01947	NM_118886.4 Latest version!	Arabidopsis thaliana 2-isopropylmalate synthase (AT4G27510), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G27510 ( NM_001125598.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001119070.1 Arabidopsis thaliana 2-isopropylmalate synthase (AT4G27510), mRNA.

Price & Availability↑

CloneID	OAb01948
Clone ID Related Accession (Same CDS sequence)	NM_001125598.1
Accession Version	NM_001125598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	2-isopropylmalate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGACGGCCA AGCGGAAGAG TAAGAAACGC CCAGCTTCGA AGGTCTCAGA TCCCGTCAAC 
TTATTCCGAT CCCTTGCTTC AGCAATTACG TCCTCTCAGG TTTCTCAGAA GCCAATTTTG 
AAACGTTTAT TGTGCCTTCT GGGTCCTTTA TCATTAACCC AACCAACAAA TTGGGAGAAT 
TGTGACATTG CTTCACATCA TTGGAATATC AAGTTCCGTG GAGAGAAAGT GGACTTGATC 
AATTTCGATG ATGTCTGTTT CCTCTCTGAT GTGTTGTTCA CAGAGCTTGA TAGGAGTCTC 
GAAAGCCTGT TCGCTACTTT ATTCAAACAA AAGGCTGAAA CTTGTCCTAC ATTTGAAAGT 
ACAGAAGATA GTATTGAATT AGCTTCGCTG TTCTTAAAAT GTTGTATGAA GATCATGTCT 
TTACTTGTGG CCAAACAAGA GCTTGTTCTG GAGAAGGCCA AAACTCTTCT CTCGATCCTC 
GGTAGATTGA TTCGTGCAAG AAATGGAGAT TGTTCTTTTG TATTCACGCA TGACGGTTCT 
TTAGATCCCC GTCATACGTT TCTTTGTACA GGGATTGAGG TTTTTATGGA TGAGATTTTG 
GTGAATAAGT CAATCAGCGA CCTTCTTTTC GTAGTTGATT CTGCATTCTC CTCATGTAGA 
CTGTTTAGTA AGCATGATAG GGCCGGTGTG GTGCAAATCG TTTCTGCTCA TTTTATTATC 
GCAACTTCTG ATGAGAAAAC GAATCAAATG TGTGTTGAGA GATTATATTG GAAGAAAGGT 
AATGCATTTA GAACTCCCCA GATAAGCTTG AGTGCTGCTG TGTCCTTGCT GCTAAACCCG 
GTTATGTTCT CTGCACCAAA AATGATCCAT GCCTATGTGG TTTTATTGGT GTCTGACGCC 
ATTGGTATTT GTTCACATCC ATGTTTTAAG GGATTAGACC TTCAGCTTAT TGATCACTAT 
ATTGATGCAT TTGAAAAGTC TGTTGTTTTG TATAAGAGAC ACATGTCTAA GAGTGAGAAT 
GGCCTGTCTG GGAAGTTTGG CTTTTTGACG AGTAAGTCTC GGGTGGCTTT TGAACATCGT 
CTTTTGCCCT CAACACTGGC GAAAGTTAAT GATGTTACAC AGCAGCTAAA GGATTCATGG 
GATTCATATC AAAGTGATAA TGCGAAGAGA GAAAACAATG AGCTGGTAGC GTATTCTGTT 
GCATACGCAA AGGAAAGCTT ATGTGTATTT GATAGCTCCT GCTCTGAAAG TATGTTGTCT 
CAGACCTTAT CAATTCTTGG TTGCGTGATA CTTAGAGCTA GTTCTGATGA TGTTATGGAC 
TCTGTGTTGG AAAAATACAA CACATCTAGT ATGGAAGATC TATATCTGCT TGCATCCACA 
CTTAAGTTCA TGACTTGTTC GATGTTGCAA GCCATCCGGG TTCTCAGGAA TTGGAACTGG 
CATGAAGCTG GAGGTGATGT TCGAGCTTGC AAGGAATACA AAGCCATGAT GGATGTTGTT 
CAACGTTTTG AACAGTTCAA TATTCATATG CCCTGTCAAA GTTTCCTGCG CGATAGAATG 
GAATCTTACC CTCACAGATC TAAGTGGATG CTTATGCATT TTTCAGGCTT ACTGTCTGTA 
AGCTTTGCAC TCAAGCTTGA CTTTCTAGTG AAGGACTCCA TTTTTGGAAT GGTGATATCT 
ATGTATTTAT TCATCCTAGA AGGGGGTGAC TTGGAAGCAC TTGGGGATTC AGTTGGTCAC 
TCAGAAAACA CGTCATCGTC TATACTATCC AGTGGTTCCA AAAACTTGGC TGCTTCTGGT 
AAAGCTGACA AGACAGCAGT AGATAGGAAA CAGAGCGGGG CAGTTGCTTT GAAGTTTCAC 
AAAAACCGAA CTTTGTACTT GGGGTATGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119070.1
CDS	36..1928
Translation	MTAKRKSKKRPASKVSDPVNLFRSLASAITSSQVSQKPILKRLLCLLGPLSLTQPTNWENCDIASHHWNIKFRGEKVDLINFDDVCFLSDVLFTELDRSLESLFATLFKQKAETCPTFESTEDSIELASLFLKCCMKIMSLLVAKQELVLEKAKTLLSILGRLIRARNGDCSFVFTHDGSLDPRHTFLCTGIEVFMDEILVNKSISDLLFVVDSAFSSCRLFSKHDRAGVVQIVSAHFIIATSDEKTNQMCVERLYWKKGNAFRTPQISLSAAVSLLLNPVMFSAPKMIHAYVVLLVSDAIGICSHPCFKGLDLQLIDHYIDAFEKSVVLYKRHMSKSENGLSGKFGFLTSKSRVAFEHRLLPSTLAKVNDVTQQLKDSWDSYQSDNAKRENNELVAYSVAYAKESLCVFDSSCSESMLSQTLSILGCVILRASSDDVMDSVLEKYNTSSMEDLYLLASTLKFMTCSMLQAIRVLRNWNWHEAGGDVRACKEYKAMMDVVQRFEQFNIHMPCQSFLRDRMESYPHRSKWMLMHFSGLLSVSFALKLDFLVKDSIFGMVISMYLFILEGGDLEALGDSVGHSENTSSSILSSGSKNLAASGKADKTAVDRKQSGAVALKFHKNRTLYLGYV

Target ORF information:

RefSeq Version	NM_001125598.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana 2-isopropylmalate synthase (AT4G27510), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125598.1

ORF Insert Sequence:

ATGACGGCCA AGCGGAAGAG TAAGAAACGC CCAGCTTCGA AGGTCTCAGA TCCCGTCAAC 
TTATTCCGAT CCCTTGCTTC AGCAATTACG TCCTCTCAGG TTTCTCAGAA GCCAATTTTG 
AAACGTTTAT TGTGCCTTCT GGGTCCTTTA TCATTAACCC AACCAACAAA TTGGGAGAAT 
TGTGACATTG CTTCACATCA TTGGAATATC AAGTTCCGTG GAGAGAAAGT GGACTTGATC 
AATTTCGATG ATGTCTGTTT CCTCTCTGAT GTGTTGTTCA CAGAGCTTGA TAGGAGTCTC 
GAAAGCCTGT TCGCTACTTT ATTCAAACAA AAGGCTGAAA CTTGTCCTAC ATTTGAAAGT 
ACAGAAGATA GTATTGAATT AGCTTCGCTG TTCTTAAAAT GTTGTATGAA GATCATGTCT 
TTACTTGTGG CCAAACAAGA GCTTGTTCTG GAGAAGGCCA AAACTCTTCT CTCGATCCTC 
GGTAGATTGA TTCGTGCAAG AAATGGAGAT TGTTCTTTTG TATTCACGCA TGACGGTTCT 
TTAGATCCCC GTCATACGTT TCTTTGTACA GGGATTGAGG TTTTTATGGA TGAGATTTTG 
GTGAATAAGT CAATCAGCGA CCTTCTTTTC GTAGTTGATT CTGCATTCTC CTCATGTAGA 
CTGTTTAGTA AGCATGATAG GGCCGGTGTG GTGCAAATCG TTTCTGCTCA TTTTATTATC 
GCAACTTCTG ATGAGAAAAC GAATCAAATG TGTGTTGAGA GATTATATTG GAAGAAAGGT 
AATGCATTTA GAACTCCCCA GATAAGCTTG AGTGCTGCTG TGTCCTTGCT GCTAAACCCG 
GTTATGTTCT CTGCACCAAA AATGATCCAT GCCTATGTGG TTTTATTGGT GTCTGACGCC 
ATTGGTATTT GTTCACATCC ATGTTTTAAG GGATTAGACC TTCAGCTTAT TGATCACTAT 
ATTGATGCAT TTGAAAAGTC TGTTGTTTTG TATAAGAGAC ACATGTCTAA GAGTGAGAAT 
GGCCTGTCTG GGAAGTTTGG CTTTTTGACG AGTAAGTCTC GGGTGGCTTT TGAACATCGT 
CTTTTGCCCT CAACACTGGC GAAAGTTAAT GATGTTACAC AGCAGCTAAA GGATTCATGG 
GATTCATATC AAAGTGATAA TGCGAAGAGA GAAAACAATG AGCTGGTAGC GTATTCTGTT 
GCATACGCAA AGGAAAGCTT ATGTGTATTT GATAGCTCCT GCTCTGAAAG TATGTTGTCT 
CAGACCTTAT CAATTCTTGG TTGCGTGATA CTTAGAGCTA GTTCTGATGA TGTTATGGAC 
TCTGTGTTGG AAAAATACAA CACATCTAGT ATGGAAGATC TATATCTGCT TGCATCCACA 
CTTAAGTTCA TGACTTGTTC GATGTTGCAA GCCATCCGGG TTCTCAGGAA TTGGAACTGG 
CATGAAGCTG GAGGTGATGT TCGAGCTTGC AAGGAATACA AAGCCATGAT GGATGTTGTT 
CAACGTTTTG AACAGTTCAA TATTCATATG CCCTGTCAAA GTTTCCTGCG CGATAGAATG 
GAATCTTACC CTCACAGATC TAAGTGGATG CTTATGCATT TTTCAGGCTT ACTGTCTGTA 
AGCTTTGCAC TCAAGCTTGA CTTTCTAGTG AAGGACTCCA TTTTTGGAAT GGTGATATCT 
ATGTATTTAT TCATCCTAGA AGGGGGTGAC TTGGAAGCAC TTGGGGATTC AGTTGGTCAC 
TCAGAAAACA CGTCATCGTC TATACTATCC AGTGGTTCCA AAAACTTGGC TGCTTCTGGT 
AAAGCTGACA AGACAGCAGT AGATAGGAAA CAGAGCGGGG CAGTTGCTTT GAAGTTTCAC 
AAAAACCGAA CTTTGTACTT GGGGTATGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G27510 ( NM_118886.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194481.2 Arabidopsis thaliana 2-isopropylmalate synthase (AT4G27510), mRNA.

Price & Availability↑

CloneID	OAb01947
Clone ID Related Accession (Same CDS sequence)	NM_118886.4
Accession Version	NM_118886.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	2-isopropylmalate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118886.3.

ATGACGGCCA AGCGGAAGAG TAAGAAACGC CCAGCTTCGA AGGTCTCAGA TCCCGTCAAC 
TTATTCCGAT CCCTTGCTTC AGCAATTACG TCCTCTCAGG TTTCTCAGAA GCCAATTTTG 
AAACGTTTAT TGTGCCTTCT GGGTCCTTTA TCATTAACCC AACCAACAAA TTGGGAGAAT 
TGTGACATTG CTTCACATCA TTGGAATATC AAGTTCCGTG GAGAGAAAGT GGACTTGATC 
AATTTCGATG ATGTCTGTTT CCTCTCTGAT GTGTTGTTCA CAGAGCTTGA TAGGAGTCTC 
GAAAGCCTGT TCGCTACTTT ATTCAAACAA AAGGCTGAAA CTTGTCCTAC ATTTGAAAGT 
ACAGAAGATA GTATTGAATT AGCTTCGCTG TTCTTAAAAT GTTGTATGAA GATCATGTCT 
TTACTTGTGG CCAAACAAGA GCTTGTTCTG GAGAAGGCCA AAACTCTTCT CTCGATCCTC 
GGTAGATTGA TTCGTGCAAG AAATGGAGAT TGTTCTTTTG TATTCACGCA TGACGGTTCT 
TTAGATCCCC GTCATACGTT TCTTTGTACA GGGATTGAGG TTTTTATGGA TGAGATTTTG 
GTGAATAAGT CAATCAGCGA CCTTCTTTTC GTAGTTGATT CTGCATTCTC CTCATGTAGA 
CTGTTTAGTA AGCATGATAG GGCCGGTGTG GTGCAAATCG TTTCTGCTCA TTTTATTATC 
GCAACTTCTG ATGAGAAAAC GAATCAAATG TGTGTTGAGA GATTATATTG GAAGAAAGGT 
AATGCATTTA GAACTCCCCA GATAAGCTTG AGTGCTGCTG TGTCCTTGCT GCTAAACCCG 
GTTATGTTCT CTGCACCAAA AATGATCCAT GCCTATGTGG TTTTATTGGT GTCTGACGCC 
ATTGGTATTT GTTCACATCC ATGTTTTAAG GGATTAGACC TTCAGCTTAT TGATCACTAT 
ATTGATGCAT TTGAAAAGTC TGTTGTTTTG TATAAGAGAC ACATGTCTAA GAGTGAGAAT 
GGCCTGTCTG GGAAGTTTGG CTTTTTGACG AGTAAGTCTC GGGTGGCTTT TGAACATCGT 
CTTTTGCCCT CAACACTGGC GAAAGTTAAT GATGTTACAC AGCAGCTAAA GGATTCATGG 
GATTCATATC AAAGTGATAA TGCGAAGAGA GAAAACAATG AGCTGGTAGC GTATTCTGTT 
GCATACGCAA AGGAAAGCTT ATGTGTATTT GATAGCTCCT GCTCTGAAAG TATGTTGTCT 
CAGACCTTAT CAATTCTTGG TTGCGTGATA CTTAGAGCTA GTTCTGATGA TGTTATGGAC 
TCTGTGTTGG AAAAATACAA CACATCTAGT ATGGAAGATC TATATCTGCT TGCATCCACA 
CTTAAGTTCA TGACTTGTTC GATGTTGCAA GCCATCCGGG TTCTCAGGAA TTGGAACTGG 
CATGAAGCTG GAGGTGATGT TCGAGCTTGC AAGGAATACA AAGCCATGAT GGATGTTGTT 
CAACGTTTTG AACAGTTCAA TATTCATATG CCCTGTCAAA GTTTCCTGCG CGATAGAATG 
GAATCTTACC CTCACAGATC TAAGTGGATG CTTATGCATT TTTCAGGCTT ACTGTCTGTA 
AGCTTTGCAC TCAAGCTTGA CTTTCTAGTG AAGGACTCCA TTTTTGGAAT GGTGATATCT 
ATGTATTTAT TCATCCTAGA AGGGGGTGAC TTGGAAGCAC TTGGGGATTC AGTTGGTCAC 
TCAGAAAACA CGTCATCGTC TATACTATCC AGTGGTTCCA AAAACTTGGC TGCTTCTGGT 
AAAGCTGACA AGACAGCAGT AGATAGGAAA CAGAGCGGGG CAGTTGCTTT GAAGTTTCAC 
AAAAACCGAA CTTTGTACTT GGGGAAGGTG TCTGAAGCTA AAGGTCCAGA GAATGGTTCA 
GATTCAGGAG TTGGTGTTGA AGAGGAAACC TGCAATGGGG AGAGATTTCT GTGGTGTATG 
GCAGGAAAAG GTAACGTGAC AAAAACCGAT GTGGACGAGT TGGCAGATTT CATAGCGTGT 
GATCCCGGTA AGGACTACTC CGACTGGCTA AAAGGGAGAG AACGATTTCG GAACCAACGG 
TGGAAGTCAG ACAAGATTGC TAAAGAAAGG TGGAATAAGA AGCAAAAAGC TTGGAGAGAG 
AACAGAGGAA GAGGGTCTAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194481.2
CDS	136..2322
Translation	MTAKRKSKKRPASKVSDPVNLFRSLASAITSSQVSQKPILKRLLCLLGPLSLTQPTNWENCDIASHHWNIKFRGEKVDLINFDDVCFLSDVLFTELDRSLESLFATLFKQKAETCPTFESTEDSIELASLFLKCCMKIMSLLVAKQELVLEKAKTLLSILGRLIRARNGDCSFVFTHDGSLDPRHTFLCTGIEVFMDEILVNKSISDLLFVVDSAFSSCRLFSKHDRAGVVQIVSAHFIIATSDEKTNQMCVERLYWKKGNAFRTPQISLSAAVSLLLNPVMFSAPKMIHAYVVLLVSDAIGICSHPCFKGLDLQLIDHYIDAFEKSVVLYKRHMSKSENGLSGKFGFLTSKSRVAFEHRLLPSTLAKVNDVTQQLKDSWDSYQSDNAKRENNELVAYSVAYAKESLCVFDSSCSESMLSQTLSILGCVILRASSDDVMDSVLEKYNTSSMEDLYLLASTLKFMTCSMLQAIRVLRNWNWHEAGGDVRACKEYKAMMDVVQRFEQFNIHMPCQSFLRDRMESYPHRSKWMLMHFSGLLSVSFALKLDFLVKDSIFGMVISMYLFILEGGDLEALGDSVGHSENTSSSILSSGSKNLAASGKADKTAVDRKQSGAVALKFHKNRTLYLGKVSEAKGPENGSDSGVGVEEETCNGERFLWCMAGKGNVTKTDVDELADFIACDPGKDYSDWLKGRERFRNQRWKSDKIAKERWNKKQKAWRENRGRGSKS

Target ORF information:

RefSeq Version	NM_118886.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana 2-isopropylmalate synthase (AT4G27510), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118886.4

ORF Insert Sequence:

ATGACGGCCA AGCGGAAGAG TAAGAAACGC CCAGCTTCGA AGGTCTCAGA TCCCGTCAAC 
TTATTCCGAT CCCTTGCTTC AGCAATTACG TCCTCTCAGG TTTCTCAGAA GCCAATTTTG 
AAACGTTTAT TGTGCCTTCT GGGTCCTTTA TCATTAACCC AACCAACAAA TTGGGAGAAT 
TGTGACATTG CTTCACATCA TTGGAATATC AAGTTCCGTG GAGAGAAAGT GGACTTGATC 
AATTTCGATG ATGTCTGTTT CCTCTCTGAT GTGTTGTTCA CAGAGCTTGA TAGGAGTCTC 
GAAAGCCTGT TCGCTACTTT ATTCAAACAA AAGGCTGAAA CTTGTCCTAC ATTTGAAAGT 
ACAGAAGATA GTATTGAATT AGCTTCGCTG TTCTTAAAAT GTTGTATGAA GATCATGTCT 
TTACTTGTGG CCAAACAAGA GCTTGTTCTG GAGAAGGCCA AAACTCTTCT CTCGATCCTC 
GGTAGATTGA TTCGTGCAAG AAATGGAGAT TGTTCTTTTG TATTCACGCA TGACGGTTCT 
TTAGATCCCC GTCATACGTT TCTTTGTACA GGGATTGAGG TTTTTATGGA TGAGATTTTG 
GTGAATAAGT CAATCAGCGA CCTTCTTTTC GTAGTTGATT CTGCATTCTC CTCATGTAGA 
CTGTTTAGTA AGCATGATAG GGCCGGTGTG GTGCAAATCG TTTCTGCTCA TTTTATTATC 
GCAACTTCTG ATGAGAAAAC GAATCAAATG TGTGTTGAGA GATTATATTG GAAGAAAGGT 
AATGCATTTA GAACTCCCCA GATAAGCTTG AGTGCTGCTG TGTCCTTGCT GCTAAACCCG 
GTTATGTTCT CTGCACCAAA AATGATCCAT GCCTATGTGG TTTTATTGGT GTCTGACGCC 
ATTGGTATTT GTTCACATCC ATGTTTTAAG GGATTAGACC TTCAGCTTAT TGATCACTAT 
ATTGATGCAT TTGAAAAGTC TGTTGTTTTG TATAAGAGAC ACATGTCTAA GAGTGAGAAT 
GGCCTGTCTG GGAAGTTTGG CTTTTTGACG AGTAAGTCTC GGGTGGCTTT TGAACATCGT 
CTTTTGCCCT CAACACTGGC GAAAGTTAAT GATGTTACAC AGCAGCTAAA GGATTCATGG 
GATTCATATC AAAGTGATAA TGCGAAGAGA GAAAACAATG AGCTGGTAGC GTATTCTGTT 
GCATACGCAA AGGAAAGCTT ATGTGTATTT GATAGCTCCT GCTCTGAAAG TATGTTGTCT 
CAGACCTTAT CAATTCTTGG TTGCGTGATA CTTAGAGCTA GTTCTGATGA TGTTATGGAC 
TCTGTGTTGG AAAAATACAA CACATCTAGT ATGGAAGATC TATATCTGCT TGCATCCACA 
CTTAAGTTCA TGACTTGTTC GATGTTGCAA GCCATCCGGG TTCTCAGGAA TTGGAACTGG 
CATGAAGCTG GAGGTGATGT TCGAGCTTGC AAGGAATACA AAGCCATGAT GGATGTTGTT 
CAACGTTTTG AACAGTTCAA TATTCATATG CCCTGTCAAA GTTTCCTGCG CGATAGAATG 
GAATCTTACC CTCACAGATC TAAGTGGATG CTTATGCATT TTTCAGGCTT ACTGTCTGTA 
AGCTTTGCAC TCAAGCTTGA CTTTCTAGTG AAGGACTCCA TTTTTGGAAT GGTGATATCT 
ATGTATTTAT TCATCCTAGA AGGGGGTGAC TTGGAAGCAC TTGGGGATTC AGTTGGTCAC 
TCAGAAAACA CGTCATCGTC TATACTATCC AGTGGTTCCA AAAACTTGGC TGCTTCTGGT 
AAAGCTGACA AGACAGCAGT AGATAGGAAA CAGAGCGGGG CAGTTGCTTT GAAGTTTCAC 
AAAAACCGAA CTTTGTACTT GGGGAAGGTG TCTGAAGCTA AAGGTCCAGA GAATGGTTCA 
GATTCAGGAG TTGGTGTTGA AGAGGAAACC TGCAATGGGG AGAGATTTCT GTGGTGTATG 
GCAGGAAAAG GTAACGTGAC AAAAACCGAT GTGGACGAGT TGGCAGATTT CATAGCGTGT 
GATCCCGGTA AGGACTACTC CGACTGGCTA AAAGGGAGAG AACGATTTCG GAACCAACGG 
TGGAAGTCAG ACAAGATTGC TAAAGAAAGG TGGAATAAGA AGCAAAAAGC TTGGAGAGAG 
AACAGAGGAA GAGGGTCTAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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AT4G27510 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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