CHX17 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHX17
Entrez Gene ID	828470
Full Name	cation/H+ exchanger 17
Synonyms	ATCHX17,F9D16.170,F9D16_170,cation/H+ exchanger 17
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001341626.1	NP_001328705.1	cation/H+ exchanger 17
NM_118501.5	NP_194101.1	cation/H+ exchanger 17

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	CHX18	NP_198976.3
Arabidopsis thaliana (thale cress)	CHX17	NP_194101.1
Oryza sativa (rice)	Os12g0616500	NP_001067282.2
Oryza sativa (rice)	Os05g0276100	NP_001055076.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The core of AtCHX17 models according to EcNhaA and TtNapA templates faces inward and outward, respectively, which may reflect two conformational states of the alternating access transport mode for proteins belonging to the plant CHX family
Title: Protein architecture and core residues in unwound ?-helices provide insights to the transport function of plant AtCHX17.

successful reproduction and seed development depend on the ability to regulate cation and pH homeostasis by AtCHX17-like transporters on membranes that traffic in the endocytic and/or secretory pathways
Title: K+ transporter AtCHX17 with its hydrophilic C tail localizes to membranes of the secretory/endocytic system: role in reproduction and seed set.

Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting.
Title: Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting.

results show that the proteins Arabidopsis thaliana Chx17 and Saccharomyces cerevisiae Kha1 could have similar functions.
Title: Arabidopsis thaliana CHX17 gene complements the kha1 deletion phenotypes in Saccharomyces cerevisiae.

AtCHX17 was expressed preferentially in epidermal and cortical cells of the mature root zones.
Title: Characterization of AtCHX17, a member of the cation/H+ exchangers, CHX family, from Arabidopsis thaliana suggests a role in K+ homeostasis.

The following CHX17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHX17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16094	NM_118501.5 Latest version!	Arabidopsis thaliana cation/H+ exchanger 17 (CHX17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb16094	NM_001341626.1 Latest version!	Arabidopsis thaliana cation/H+ exchanger 17 (CHX17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHX17 ( NM_001341626.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328705.1 Arabidopsis thaliana cation/H+ exchanger 17 (CHX17), mRNA.

Price & Availability↑

CloneID	OAb16094
Clone ID Related Accession (Same CDS sequence)	NM_001341626.1 , NM_118501.5
Accession Version	NM_001341626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cation/H+ exchanger 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGGAACAA ACGGTACAAC ATGTCCAGGA CCAATGAAAG CAACCTCTAA TGGAGTCTTT 
CAAGGAGAGA ATCCTCTGGA ACACGCGTTG CCTCTTCTCA TACTCCAGAT CTGTATCGTT 
CTTCTTCTCA CTCGTCTTCT TGCTTTTCTC CTCCGTCCTC TCCGGCAGCC ACGTGTCATC 
GCCGAGATTG TCGGTGGAAT ATTACTTGGA CCATCAGCAC TTGGGAAAAG CACTAAGTTT 
ATCAACACAG TTTTCCCACC AAAGAGTCTA ACGGTTTTAG ACACACTTGC AAACTTAGGA 
CTCATCTTCT TCCTCTTCCT CGTTGGTCTC GAGCTCGATC CCAAATCTCT TAAGCGAACC 
GGAAAGAGAG CTCTCTCTAT CGCTCTCGCC GGAATCACTC TTCCTTTCGT ATTAGGCATT 
GGAACTTCTT TTGCTCTCCG CAGTTCCATA GCTGATGGAG CCAGCAAAGC TCCTTTCCTT 
GTCTTCATGG GTGTTGCTCT CTCCATCACA GCGTTTCCCG TCTTGGCCCG AATACTCGCT 
GAGATTAAGC TTCTAACCAC TGATATCGGA AAGATTGCTT TGTCCGCTGC TGCGGTCAAT 
GACGTGGCTG CTTGGATTCT CCTTGCTCTC GCGGTGGCTC TTTCTGGAGA GGGGAGCTCT 
CCGCTTACTT CTCTTTGGGT GTTTCTCTCG GGTTGCGGTT TTGTCTTGTT CTGTATCTTT 
GTTGTGCAAC CAGGGATTAA GTTGATTGCT AAACGTTGCC CTGAAGGAGA ACCGGTAAAT 
GAACTTTACG TGTGTTGCAC GTTAGGTATT GTTCTTGCAG CTAGCTTCGT CACAGACTTT 
ATTGGGATTC ATGCGCTGTT TGGTGCTTTT GTGATCGGGG TTATTTTCCC TAAAGAAGGC 
AATTTCGCGA ATGCTTTAGT TGAGAAAGTT GAGGATCTCG TGTCAGGTCT TTTCTTGCCG 
CTCTACTTCG TCTCAAGTGG TTTAAAGACC AATGTTGCGA CGATCCAAGG AGCTCAGTCT 
TGGGGACTCT TGGTTTTGGT TATATTTAAT GCTTGTTTCG GTAAAATCAT CGGTACAGTC 
CTAGTTTCCC TTTACTGCAA AGTTCCGCTT GACCAGTCTT TAGCGCTTGG TTTCTTGATG 
AACACGAAAG GTCTTGTTGA GCTCATCGTC CTCAACATTG GTAAAGACAG AGGGGTTCTG 
AATGATCAAA TTTTCGCTAT AATGGTTCTA ATGGCGATAT TCACCACATT CATGACGACT 
CCTCTAGTTC TGGCAGTGTA CAAACCGGGT AAATCCTTAA CCAAAGCTGA TTATAAGAAC 
CGAACGGTGG AGGAGACGAA CCGTTCAAAC AAACCGCTCT GCCTCATGTT CTGTTTCCAG 
AGCATAATGA ACATCCCCAC GATCGTCAAC CTCATAGAAG CATCCCGAGG CATAAACCGT 
AAAGAAAATC TATCAGTCTA CGCGATGCAT CTTATGGAGC TTTCAGAGAG ATCATCGGCT 
ATACTCATGG CACACAAGGT CAGGAGAAAC GGTCTTCCCT TCTGGAACAA AGACAAATCT 
GAGAACAATA GTTCTTCTTC CGACATGGTT GTGGTCGCAT TCGAGGCTTT CCGAAGACTC 
AGCCGTGTCT CAGTGCGTCC CATGACAGCG ATCTCACCGA TGGCGACTAT ACATGAGGAT 
ATTTGCCAAA GCGCGGAACG CAAAAAAACC GCTATGGTGA TTCTTCCGTT TCATAAGCAC 
GTTAGGCTGG ACAGAACATG GGAGACGACT AGGAATGACT ACCGTTGGAT TAACAAGAAG 
GTTATGGAGG AATCTCCATG TTCTGTTGCG ATTTTGGTTG ATCGTGGACT TGGTGGTACG 
ACACGTGTCG CGTCTAGTGA TTTCTCGTTG ACTATAACAG TTTTGTTTTT TGGAGGGAAT 
GATGATCGTG AGGCGTTAGC GTTTGCGGTG CGAATGGCTG AACATCCCGG GATAAGCTTA 
ACCGTTGTTC GGTTTATCCC TAGCGATGAA TTCAAACCGG AAAACGTGAG GATCGAGATA 
ACCGAAGATC AGCTCTGTTC AGGGGCAACT AGATTGATTG ATATTGAAGC TATAACCGAA 
CTTAAAGCAA AGATTAAAGA AAAAGAAAGT TCTCGTTCTA ATTCGGATTC TGAATCTCAT 
ATCATTTACG AAGAGAAAAT CGTGAAATGT TACGAGGAAG TTATCGAAGT TATTAAAGAG 
TATTCTAAAA GCAACCTTTT CTTGGTGGGG AAGTCACCGG AAGGTTCTGT AGCGTCAGGG 
ATAAACGTAA GAAGCGACAC GCCTGAGCTT GGACCGATCG GGAATTTGTT AACGGAAAGC 
GAAAGTGTTT CGACGGTGGC ATCAGTGTTG GTGGTGCAAC AGTATATAGC AAGCCGTCCG 
GTCGGAATTT CGAAGAACGT CACGACTGAA GAGTCGCTGG TTGAGGATTC TGAGAGTCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328705.1
CDS	37..2499
Translation	MGTNGTTCPGPMKATSNGVFQGENPLEHALPLLILQICIVLLLTRLLAFLLRPLRQPRVIAEIVGGILLGPSALGKSTKFINTVFPPKSLTVLDTLANLGLIFFLFLVGLELDPKSLKRTGKRALSIALAGITLPFVLGIGTSFALRSSIADGASKAPFLVFMGVALSITAFPVLARILAEIKLLTTDIGKIALSAAAVNDVAAWILLALAVALSGEGSSPLTSLWVFLSGCGFVLFCIFVVQPGIKLIAKRCPEGEPVNELYVCCTLGIVLAASFVTDFIGIHALFGAFVIGVIFPKEGNFANALVEKVEDLVSGLFLPLYFVSSGLKTNVATIQGAQSWGLLVLVIFNACFGKIIGTVLVSLYCKVPLDQSLALGFLMNTKGLVELIVLNIGKDRGVLNDQIFAIMVLMAIFTTFMTTPLVLAVYKPGKSLTKADYKNRTVEETNRSNKPLCLMFCFQSIMNIPTIVNLIEASRGINRKENLSVYAMHLMELSERSSAILMAHKVRRNGLPFWNKDKSENNSSSSDMVVVAFEAFRRLSRVSVRPMTAISPMATIHEDICQSAERKKTAMVILPFHKHVRLDRTWETTRNDYRWINKKVMEESPCSVAILVDRGLGGTTRVASSDFSLTITVLFFGGNDDREALAFAVRMAEHPGISLTVVRFIPSDEFKPENVRIEITEDQLCSGATRLIDIEAITELKAKIKEKESSRSNSDSESHIIYEEKIVKCYEEVIEVIKEYSKSNLFLVGKSPEGSVASGINVRSDTPELGPIGNLLTESESVSTVASVLVVQQYIASRPVGISKNVTTEESLVEDSESP

Target ORF information:

RefSeq Version	NM_001341626.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cation/H+ exchanger 17 (CHX17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341626.1

ORF Insert Sequence:

ATGGGAACAA ACGGTACAAC ATGTCCAGGA CCAATGAAAG CAACCTCTAA TGGAGTCTTT 
CAAGGAGAGA ATCCTCTGGA ACACGCGTTG CCTCTTCTCA TACTCCAGAT CTGTATCGTT 
CTTCTTCTCA CTCGTCTTCT TGCTTTTCTC CTCCGTCCTC TCCGGCAGCC ACGTGTCATC 
GCCGAGATTG TCGGTGGAAT ATTACTTGGA CCATCAGCAC TTGGGAAAAG CACTAAGTTT 
ATCAACACAG TTTTCCCACC AAAGAGTCTA ACGGTTTTAG ACACACTTGC AAACTTAGGA 
CTCATCTTCT TCCTCTTCCT CGTTGGTCTC GAGCTCGATC CCAAATCTCT TAAGCGAACC 
GGAAAGAGAG CTCTCTCTAT CGCTCTCGCC GGAATCACTC TTCCTTTCGT ATTAGGCATT 
GGAACTTCTT TTGCTCTCCG CAGTTCCATA GCTGATGGAG CCAGCAAAGC TCCTTTCCTT 
GTCTTCATGG GTGTTGCTCT CTCCATCACA GCGTTTCCCG TCTTGGCCCG AATACTCGCT 
GAGATTAAGC TTCTAACCAC TGATATCGGA AAGATTGCTT TGTCCGCTGC TGCGGTCAAT 
GACGTGGCTG CTTGGATTCT CCTTGCTCTC GCGGTGGCTC TTTCTGGAGA GGGGAGCTCT 
CCGCTTACTT CTCTTTGGGT GTTTCTCTCG GGTTGCGGTT TTGTCTTGTT CTGTATCTTT 
GTTGTGCAAC CAGGGATTAA GTTGATTGCT AAACGTTGCC CTGAAGGAGA ACCGGTAAAT 
GAACTTTACG TGTGTTGCAC GTTAGGTATT GTTCTTGCAG CTAGCTTCGT CACAGACTTT 
ATTGGGATTC ATGCGCTGTT TGGTGCTTTT GTGATCGGGG TTATTTTCCC TAAAGAAGGC 
AATTTCGCGA ATGCTTTAGT TGAGAAAGTT GAGGATCTCG TGTCAGGTCT TTTCTTGCCG 
CTCTACTTCG TCTCAAGTGG TTTAAAGACC AATGTTGCGA CGATCCAAGG AGCTCAGTCT 
TGGGGACTCT TGGTTTTGGT TATATTTAAT GCTTGTTTCG GTAAAATCAT CGGTACAGTC 
CTAGTTTCCC TTTACTGCAA AGTTCCGCTT GACCAGTCTT TAGCGCTTGG TTTCTTGATG 
AACACGAAAG GTCTTGTTGA GCTCATCGTC CTCAACATTG GTAAAGACAG AGGGGTTCTG 
AATGATCAAA TTTTCGCTAT AATGGTTCTA ATGGCGATAT TCACCACATT CATGACGACT 
CCTCTAGTTC TGGCAGTGTA CAAACCGGGT AAATCCTTAA CCAAAGCTGA TTATAAGAAC 
CGAACGGTGG AGGAGACGAA CCGTTCAAAC AAACCGCTCT GCCTCATGTT CTGTTTCCAG 
AGCATAATGA ACATCCCCAC GATCGTCAAC CTCATAGAAG CATCCCGAGG CATAAACCGT 
AAAGAAAATC TATCAGTCTA CGCGATGCAT CTTATGGAGC TTTCAGAGAG ATCATCGGCT 
ATACTCATGG CACACAAGGT CAGGAGAAAC GGTCTTCCCT TCTGGAACAA AGACAAATCT 
GAGAACAATA GTTCTTCTTC CGACATGGTT GTGGTCGCAT TCGAGGCTTT CCGAAGACTC 
AGCCGTGTCT CAGTGCGTCC CATGACAGCG ATCTCACCGA TGGCGACTAT ACATGAGGAT 
ATTTGCCAAA GCGCGGAACG CAAAAAAACC GCTATGGTGA TTCTTCCGTT TCATAAGCAC 
GTTAGGCTGG ACAGAACATG GGAGACGACT AGGAATGACT ACCGTTGGAT TAACAAGAAG 
GTTATGGAGG AATCTCCATG TTCTGTTGCG ATTTTGGTTG ATCGTGGACT TGGTGGTACG 
ACACGTGTCG CGTCTAGTGA TTTCTCGTTG ACTATAACAG TTTTGTTTTT TGGAGGGAAT 
GATGATCGTG AGGCGTTAGC GTTTGCGGTG CGAATGGCTG AACATCCCGG GATAAGCTTA 
ACCGTTGTTC GGTTTATCCC TAGCGATGAA TTCAAACCGG AAAACGTGAG GATCGAGATA 
ACCGAAGATC AGCTCTGTTC AGGGGCAACT AGATTGATTG ATATTGAAGC TATAACCGAA 
CTTAAAGCAA AGATTAAAGA AAAAGAAAGT TCTCGTTCTA ATTCGGATTC TGAATCTCAT 
ATCATTTACG AAGAGAAAAT CGTGAAATGT TACGAGGAAG TTATCGAAGT TATTAAAGAG 
TATTCTAAAA GCAACCTTTT CTTGGTGGGG AAGTCACCGG AAGGTTCTGT AGCGTCAGGG 
ATAAACGTAA GAAGCGACAC GCCTGAGCTT GGACCGATCG GGAATTTGTT AACGGAAAGC 
GAAAGTGTTT CGACGGTGGC ATCAGTGTTG GTGGTGCAAC AGTATATAGC AAGCCGTCCG 
GTCGGAATTT CGAAGAACGT CACGACTGAA GAGTCGCTGG TTGAGGATTC TGAGAGTCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHX17 ( NM_118501.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194101.1 Arabidopsis thaliana cation/H+ exchanger 17 (CHX17), mRNA.

Price & Availability↑

CloneID	OAb16094
Clone ID Related Accession (Same CDS sequence)	NM_001341626.1 , NM_118501.5
Accession Version	NM_118501.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cation/H+ exchanger 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118501.4.

ATGGGAACAA ACGGTACAAC ATGTCCAGGA CCAATGAAAG CAACCTCTAA TGGAGTCTTT 
CAAGGAGAGA ATCCTCTGGA ACACGCGTTG CCTCTTCTCA TACTCCAGAT CTGTATCGTT 
CTTCTTCTCA CTCGTCTTCT TGCTTTTCTC CTCCGTCCTC TCCGGCAGCC ACGTGTCATC 
GCCGAGATTG TCGGTGGAAT ATTACTTGGA CCATCAGCAC TTGGGAAAAG CACTAAGTTT 
ATCAACACAG TTTTCCCACC AAAGAGTCTA ACGGTTTTAG ACACACTTGC AAACTTAGGA 
CTCATCTTCT TCCTCTTCCT CGTTGGTCTC GAGCTCGATC CCAAATCTCT TAAGCGAACC 
GGAAAGAGAG CTCTCTCTAT CGCTCTCGCC GGAATCACTC TTCCTTTCGT ATTAGGCATT 
GGAACTTCTT TTGCTCTCCG CAGTTCCATA GCTGATGGAG CCAGCAAAGC TCCTTTCCTT 
GTCTTCATGG GTGTTGCTCT CTCCATCACA GCGTTTCCCG TCTTGGCCCG AATACTCGCT 
GAGATTAAGC TTCTAACCAC TGATATCGGA AAGATTGCTT TGTCCGCTGC TGCGGTCAAT 
GACGTGGCTG CTTGGATTCT CCTTGCTCTC GCGGTGGCTC TTTCTGGAGA GGGGAGCTCT 
CCGCTTACTT CTCTTTGGGT GTTTCTCTCG GGTTGCGGTT TTGTCTTGTT CTGTATCTTT 
GTTGTGCAAC CAGGGATTAA GTTGATTGCT AAACGTTGCC CTGAAGGAGA ACCGGTAAAT 
GAACTTTACG TGTGTTGCAC GTTAGGTATT GTTCTTGCAG CTAGCTTCGT CACAGACTTT 
ATTGGGATTC ATGCGCTGTT TGGTGCTTTT GTGATCGGGG TTATTTTCCC TAAAGAAGGC 
AATTTCGCGA ATGCTTTAGT TGAGAAAGTT GAGGATCTCG TGTCAGGTCT TTTCTTGCCG 
CTCTACTTCG TCTCAAGTGG TTTAAAGACC AATGTTGCGA CGATCCAAGG AGCTCAGTCT 
TGGGGACTCT TGGTTTTGGT TATATTTAAT GCTTGTTTCG GTAAAATCAT CGGTACAGTC 
CTAGTTTCCC TTTACTGCAA AGTTCCGCTT GACCAGTCTT TAGCGCTTGG TTTCTTGATG 
AACACGAAAG GTCTTGTTGA GCTCATCGTC CTCAACATTG GTAAAGACAG AGGGGTTCTG 
AATGATCAAA TTTTCGCTAT AATGGTTCTA ATGGCGATAT TCACCACATT CATGACGACT 
CCTCTAGTTC TGGCAGTGTA CAAACCGGGT AAATCCTTAA CCAAAGCTGA TTATAAGAAC 
CGAACGGTGG AGGAGACGAA CCGTTCAAAC AAACCGCTCT GCCTCATGTT CTGTTTCCAG 
AGCATAATGA ACATCCCCAC GATCGTCAAC CTCATAGAAG CATCCCGAGG CATAAACCGT 
AAAGAAAATC TATCAGTCTA CGCGATGCAT CTTATGGAGC TTTCAGAGAG ATCATCGGCT 
ATACTCATGG CACACAAGGT CAGGAGAAAC GGTCTTCCCT TCTGGAACAA AGACAAATCT 
GAGAACAATA GTTCTTCTTC CGACATGGTT GTGGTCGCAT TCGAGGCTTT CCGAAGACTC 
AGCCGTGTCT CAGTGCGTCC CATGACAGCG ATCTCACCGA TGGCGACTAT ACATGAGGAT 
ATTTGCCAAA GCGCGGAACG CAAAAAAACC GCTATGGTGA TTCTTCCGTT TCATAAGCAC 
GTTAGGCTGG ACAGAACATG GGAGACGACT AGGAATGACT ACCGTTGGAT TAACAAGAAG 
GTTATGGAGG AATCTCCATG TTCTGTTGCG ATTTTGGTTG ATCGTGGACT TGGTGGTACG 
ACACGTGTCG CGTCTAGTGA TTTCTCGTTG ACTATAACAG TTTTGTTTTT TGGAGGGAAT 
GATGATCGTG AGGCGTTAGC GTTTGCGGTG CGAATGGCTG AACATCCCGG GATAAGCTTA 
ACCGTTGTTC GGTTTATCCC TAGCGATGAA TTCAAACCGG AAAACGTGAG GATCGAGATA 
ACCGAAGATC AGCTCTGTTC AGGGGCAACT AGATTGATTG ATATTGAAGC TATAACCGAA 
CTTAAAGCAA AGATTAAAGA AAAAGAAAGT TCTCGTTCTA ATTCGGATTC TGAATCTCAT 
ATCATTTACG AAGAGAAAAT CGTGAAATGT TACGAGGAAG TTATCGAAGT TATTAAAGAG 
TATTCTAAAA GCAACCTTTT CTTGGTGGGG AAGTCACCGG AAGGTTCTGT AGCGTCAGGG 
ATAAACGTAA GAAGCGACAC GCCTGAGCTT GGACCGATCG GGAATTTGTT AACGGAAAGC 
GAAAGTGTTT CGACGGTGGC ATCAGTGTTG GTGGTGCAAC AGTATATAGC AAGCCGTCCG 
GTCGGAATTT CGAAGAACGT CACGACTGAA GAGTCGCTGG TTGAGGATTC TGAGAGTCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194101.1
CDS	172..2634
Translation	MGTNGTTCPGPMKATSNGVFQGENPLEHALPLLILQICIVLLLTRLLAFLLRPLRQPRVIAEIVGGILLGPSALGKSTKFINTVFPPKSLTVLDTLANLGLIFFLFLVGLELDPKSLKRTGKRALSIALAGITLPFVLGIGTSFALRSSIADGASKAPFLVFMGVALSITAFPVLARILAEIKLLTTDIGKIALSAAAVNDVAAWILLALAVALSGEGSSPLTSLWVFLSGCGFVLFCIFVVQPGIKLIAKRCPEGEPVNELYVCCTLGIVLAASFVTDFIGIHALFGAFVIGVIFPKEGNFANALVEKVEDLVSGLFLPLYFVSSGLKTNVATIQGAQSWGLLVLVIFNACFGKIIGTVLVSLYCKVPLDQSLALGFLMNTKGLVELIVLNIGKDRGVLNDQIFAIMVLMAIFTTFMTTPLVLAVYKPGKSLTKADYKNRTVEETNRSNKPLCLMFCFQSIMNIPTIVNLIEASRGINRKENLSVYAMHLMELSERSSAILMAHKVRRNGLPFWNKDKSENNSSSSDMVVVAFEAFRRLSRVSVRPMTAISPMATIHEDICQSAERKKTAMVILPFHKHVRLDRTWETTRNDYRWINKKVMEESPCSVAILVDRGLGGTTRVASSDFSLTITVLFFGGNDDREALAFAVRMAEHPGISLTVVRFIPSDEFKPENVRIEITEDQLCSGATRLIDIEAITELKAKIKEKESSRSNSDSESHIIYEEKIVKCYEEVIEVIKEYSKSNLFLVGKSPEGSVASGINVRSDTPELGPIGNLLTESESVSTVASVLVVQQYIASRPVGISKNVTTEESLVEDSESP

Target ORF information:

RefSeq Version	NM_118501.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cation/H+ exchanger 17 (CHX17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118501.5

ORF Insert Sequence:

ATGGGAACAA ACGGTACAAC ATGTCCAGGA CCAATGAAAG CAACCTCTAA TGGAGTCTTT 
CAAGGAGAGA ATCCTCTGGA ACACGCGTTG CCTCTTCTCA TACTCCAGAT CTGTATCGTT 
CTTCTTCTCA CTCGTCTTCT TGCTTTTCTC CTCCGTCCTC TCCGGCAGCC ACGTGTCATC 
GCCGAGATTG TCGGTGGAAT ATTACTTGGA CCATCAGCAC TTGGGAAAAG CACTAAGTTT 
ATCAACACAG TTTTCCCACC AAAGAGTCTA ACGGTTTTAG ACACACTTGC AAACTTAGGA 
CTCATCTTCT TCCTCTTCCT CGTTGGTCTC GAGCTCGATC CCAAATCTCT TAAGCGAACC 
GGAAAGAGAG CTCTCTCTAT CGCTCTCGCC GGAATCACTC TTCCTTTCGT ATTAGGCATT 
GGAACTTCTT TTGCTCTCCG CAGTTCCATA GCTGATGGAG CCAGCAAAGC TCCTTTCCTT 
GTCTTCATGG GTGTTGCTCT CTCCATCACA GCGTTTCCCG TCTTGGCCCG AATACTCGCT 
GAGATTAAGC TTCTAACCAC TGATATCGGA AAGATTGCTT TGTCCGCTGC TGCGGTCAAT 
GACGTGGCTG CTTGGATTCT CCTTGCTCTC GCGGTGGCTC TTTCTGGAGA GGGGAGCTCT 
CCGCTTACTT CTCTTTGGGT GTTTCTCTCG GGTTGCGGTT TTGTCTTGTT CTGTATCTTT 
GTTGTGCAAC CAGGGATTAA GTTGATTGCT AAACGTTGCC CTGAAGGAGA ACCGGTAAAT 
GAACTTTACG TGTGTTGCAC GTTAGGTATT GTTCTTGCAG CTAGCTTCGT CACAGACTTT 
ATTGGGATTC ATGCGCTGTT TGGTGCTTTT GTGATCGGGG TTATTTTCCC TAAAGAAGGC 
AATTTCGCGA ATGCTTTAGT TGAGAAAGTT GAGGATCTCG TGTCAGGTCT TTTCTTGCCG 
CTCTACTTCG TCTCAAGTGG TTTAAAGACC AATGTTGCGA CGATCCAAGG AGCTCAGTCT 
TGGGGACTCT TGGTTTTGGT TATATTTAAT GCTTGTTTCG GTAAAATCAT CGGTACAGTC 
CTAGTTTCCC TTTACTGCAA AGTTCCGCTT GACCAGTCTT TAGCGCTTGG TTTCTTGATG 
AACACGAAAG GTCTTGTTGA GCTCATCGTC CTCAACATTG GTAAAGACAG AGGGGTTCTG 
AATGATCAAA TTTTCGCTAT AATGGTTCTA ATGGCGATAT TCACCACATT CATGACGACT 
CCTCTAGTTC TGGCAGTGTA CAAACCGGGT AAATCCTTAA CCAAAGCTGA TTATAAGAAC 
CGAACGGTGG AGGAGACGAA CCGTTCAAAC AAACCGCTCT GCCTCATGTT CTGTTTCCAG 
AGCATAATGA ACATCCCCAC GATCGTCAAC CTCATAGAAG CATCCCGAGG CATAAACCGT 
AAAGAAAATC TATCAGTCTA CGCGATGCAT CTTATGGAGC TTTCAGAGAG ATCATCGGCT 
ATACTCATGG CACACAAGGT CAGGAGAAAC GGTCTTCCCT TCTGGAACAA AGACAAATCT 
GAGAACAATA GTTCTTCTTC CGACATGGTT GTGGTCGCAT TCGAGGCTTT CCGAAGACTC 
AGCCGTGTCT CAGTGCGTCC CATGACAGCG ATCTCACCGA TGGCGACTAT ACATGAGGAT 
ATTTGCCAAA GCGCGGAACG CAAAAAAACC GCTATGGTGA TTCTTCCGTT TCATAAGCAC 
GTTAGGCTGG ACAGAACATG GGAGACGACT AGGAATGACT ACCGTTGGAT TAACAAGAAG 
GTTATGGAGG AATCTCCATG TTCTGTTGCG ATTTTGGTTG ATCGTGGACT TGGTGGTACG 
ACACGTGTCG CGTCTAGTGA TTTCTCGTTG ACTATAACAG TTTTGTTTTT TGGAGGGAAT 
GATGATCGTG AGGCGTTAGC GTTTGCGGTG CGAATGGCTG AACATCCCGG GATAAGCTTA 
ACCGTTGTTC GGTTTATCCC TAGCGATGAA TTCAAACCGG AAAACGTGAG GATCGAGATA 
ACCGAAGATC AGCTCTGTTC AGGGGCAACT AGATTGATTG ATATTGAAGC TATAACCGAA 
CTTAAAGCAA AGATTAAAGA AAAAGAAAGT TCTCGTTCTA ATTCGGATTC TGAATCTCAT 
ATCATTTACG AAGAGAAAAT CGTGAAATGT TACGAGGAAG TTATCGAAGT TATTAAAGAG 
TATTCTAAAA GCAACCTTTT CTTGGTGGGG AAGTCACCGG AAGGTTCTGT AGCGTCAGGG 
ATAAACGTAA GAAGCGACAC GCCTGAGCTT GGACCGATCG GGAATTTGTT AACGGAAAGC 
GAAAGTGTTT CGACGGTGGC ATCAGTGTTG GTGGTGCAAC AGTATATAGC AAGCCGTCCG 
GTCGGAATTT CGAAGAACGT CACGACTGAA GAGTCGCTGG TTGAGGATTC TGAGAGTCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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CHX17 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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