ESP cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESP
Entrez Gene ID	828396
Full Name	separase
Synonyms	AESP,EXTRA SPINDLE POLES,F7H19.150,F7H19_150,RADIALLY SWOLLEN 4,RSW4,SEPARASE,homolog of separase
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001203875.2	NP_001190804.1	separase
NM_118426.4	NP_194028.2	separase
NM_001341570.1	NP_001328112.1	separase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0770700	NP_001048252.1
Arabidopsis thaliana (thale cress)	ESP	NP_194028.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure of the Arabidopsis thaliana ESP protein has been reported.
Title: Crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana provides insights into its product specificity.

the N-terminal non-catalytic domain of separase binds to the C-terminal tail domain of three homologs of the centromeric protein CENP-E Kinesin 7.
Title: Separase Promotes Microtubule Polymerization by Activating CENP-E-Related Kinesin Kin7.

Data indicate that caspase-related protease separase (extra spindle poles [ESP]) colocalizes with microtubules and RabA2a, and facilitates polar targeting of the auxin efflux carrier PIN-formed2 (PIN2) to the rootward side of the root cortex cell.
Title: The caspase-related protease separase (extra spindle poles) regulates cell polarity and cytokinesis in Arabidopsis.

Plant separase, in addition to cleaving cohesin, regulates cyclin B1;1, with profound ramifications for morphogenesis.
Title: A conditional mutation in Arabidopsis thaliana separase induces chromosome non-disjunction, aberrant morphogenesis and cyclin B1;1 stability.

It was shown that meiotic expression of ESP blocked the removal of cohesin during meiosis, resulted in alterations in centromere association, disrupted the radial microtubule system, and affected the establishment of nuclear cytoplasmic domains.
Title: Arabidopsis separase functions beyond the removal of sister chromatid cohesion during meiosis.

The following ESP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb16017	NM_118426.4 Latest version!	Arabidopsis thaliana separase (ESP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OAb30163	NM_001203875.2 Latest version!	Arabidopsis thaliana separase (ESP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OAb39612	NM_001341570.1 Latest version!	Arabidopsis thaliana separase (ESP), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESP ( NM_118426.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194028.2 Arabidopsis thaliana separase (ESP), mRNA.

Price & Availability↑

CloneID	OAb16017
Clone ID Related Accession (Same CDS sequence)	NM_118426.4
Accession Version	NM_118426.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	separase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118426.3.

ATGGCGTCCT CTGGCGACGA CCTCCGTCTT CTCTCTCTCA TCGACGTCGG CGATAATGTC 
TTCTCTTCCT TCTCCGATTA CCTTAAACCT TTCTCTACTC TATCCACTTC CCGGAAAAAA 
CAAGACCGAG CAACCACGAT TCGAGCCCTA GCTAAGCAAT TCCTTCCTTT CCTCAACAAA 
TCGATCTCTC TCCTACCGAA ACGTCTCTCC GTCGCGAATT CTGATAAGGA AGCTCGTGAA 
TCAGCATTAG ATCTGTTCCG TGCTTATGAA CTTTGTTTGG ACTGTTTAGA ATTGGTCTCT 
GCTCAATTAG CTTGTAAGCC TCACACTGTT CAATCTCAGA GGCTAAGGAT GATTCATTGT 
CTCGATGTTT GGGGTTTGTA TGAGAATGTG TATACTGAAG CGTTTAAGGT TCTGGAGAAG 
CTAAGAGGCT CTGATAGCAA ATCCCGGAAG TCCCGGTTGT TGCCGGAGGT TCAGGATGGA 
GATGCGGAGA TGGCTTTAGT TGTTGTTGAT GCTGTGGCGG CCATCTTTAG GGCTGTGGCA 
ATGAGTCAGC AGTTAGATGA TAAGCGGTAT CGGAAAGTTC TTCTTTTGCT TGAGGAAGTC 
GGAGGCTGGT TAAGGGTCTT AGATGCTAAG GTGTATGAAA AGTTGCACAG AGCGATGGTG 
ACAAGTATGG GTAAATGTGC TGTTTCTCTC GTTAGAGAAG CTGAGCGTTT CAATGGGGAT 
TTAGTGATTT CTTTCTGCGA CTTAACTGTG AAGGAACATT ATAAATCTGC ATTATCAAAA 
GATCGAGTTT ATAAGTTTGC TCGCGAGGTG CTTTCTGTTC TGTTTGGATT TAAGGATAGA 
AAAATGTCAG TGACCATTGA CATTTCAATG TCTGTGTTGC GCAGCTTATC TTGCCAATTT 
GAGGATGAAA GTAATGAGAA TTTAATGGAG TTTTTTGACC TAGTCGATTA TTGCGCTCAC 
AAGTTTCGAG CAGCAGGAGA CATGTACTGT GCAAAAGTTT CAAAAAAATT GAATGAGATG 
GCAGCTATTT TCGTAGAGGC AATACCCCAA CTTAATTTGG TTCTTAGACT GTATTCGACC 
GGGCTGTCCA TCACGGTTTG CAATTCCAAG CTCGGAGAAA TTAAGCTAGA AGATTCAACA 
GATGATTGGA AGATTCAAGC TATGTTTGAT GATGATGCTA GATGGCAAAG CTTAGTTTCT 
TTACTCGGCA TGGTTGATAG TTACTCAGGT GATGAGGGCA ATCAGACTGG TTCATCATCG 
ATTGGTGGGC ATAGGAACTA CAACAATAAA ACACATGATA GCTGTAAAGA CAGGAACAAG 
ATTACTTGTT GGCCACAATA CGTAGATGCC TTGAAATTCT TGTGCCAACC GCTTGCAGAC 
TTTATATATT CTGTGAAAAG AAAGATTGTG CTGGAGACAG AAATGTCATG CGCCTCTGCT 
CATCTAATTA CTATTCACGA TGCCTTTCTC CAGTTTTGTG ATGGCTGTCT CTTCCTTCAG 
AGGTGCACGT CTGACAAGGG AGATAGAGAA ATTGCCAATA ACAAAGCTTT CTTAAACGCG 
GCTATGGGTG CTTTCATTGT CTCATTGAGA ACCCAACTTA AATTGGAGAT AAGTGCCCAT 
CTAGTTGAGG ATGTAATTGG AAGCCCATGG ATCCAATCTC AAGAACTCAA GTATCTAATT 
GCGACGCTGT ACAATATTGG TATCGTTTTG TACAGAAATA AGGAGCTAAA CAAGGCTTGT 
GAGGCGCTTA AGCTGTGCTC CAAGGTATCA TGGAGATGTG TTGAACTACA TTGTCATATG 
TTTGTAAATC AGTCTAGTTC ATCTGATAAT GACTTGTCAG AAGATGCCAT TATGGATTTT 
GTGGGCGAAG CATGTAATAG GTGTGCATTC TACCTGGATA TACTCCAGAA ATGTAGCAGA 
CGTAAGATTA GACAGAATAT TGTTCATATT CTAGAAAATT GGTTGTCAGC TGAACATCTG 
ATTAGACGGT TACCAGGCCC TGAGGCAATT GTAAAGCAAT GGGTTAAGAT AGAGCGGGAA 
TGTCACACGG ATCTGGATGC GGCAGGTTCT TGTACAACTT TGTACTCATT GTTATCATCT 
TCTCAAAAAA AGTCAAAGCG GGGTATCGGA AAAATCCTTG AGCAGGAGCT TCTTGCCTAT 
GACAGAGTGT TACCTTTAAG GTCAAATCTA GGTCAACAAA CGCGAATCAA GATAGCAGAT 
ATCCTCTTGA AGGATGTTTA TGTCACTGAG GATATGCATA TAGAGAGAGC AAGAATTTTG 
ATATGGAAAG CACGAATGAC AAGGACATCT GGAACTGAAC ATATAACCGA GTGCATTTGT 
TTTCTGTCTG AAGCAATCTC TATATTGGGT GAGTTACATC ATGGGCCAAA TGAAGAGGGT 
TCTCCATCTT CTCACATGTT ACCTATTGCG TATTGCTTGA GAGCTTTTTG TACCCAGGAA 
GCTGACCCAA ACTCGAAGAA AGTCTTTCAA GATATTAGCA CTTCACTAAA TCTCTGGCTA 
AGGATTCTTA GCCTAGATGA TAGTGGAGAC AGTCTACCAA CAGAGAATAT AATTCCGTTA 
CTGTATAATA TGATTGATTT GATGTCAGTG AAGGGATGTA CAGAGCTCCA TCATCATATA 
TACCAACTGA TTTTCAGATT GTTTAAATGG AAGAATGTCA AATTGGAGGT CTGCCTTGCA 
ATGCTGTGGG AATGTAGAAG GCTTAGTCAT GCCTTATGCC CTTCCCCCAT AAGTGATGCC 
TTTATCCAGA CCTTATCAGA GAATTGTGCT GACAAATCCA CATGCATTGA TTTCTGGATG 
GATTGCCTGA AAGATTCAAA AGCAAAGTTA ATTGGGTTCC AGCAGAACTT CCATGATTTA 
CATAATAAGG ATGAAGGTCC CTTTCAATCA GACATCACAA TTGACGATAT TAAAGATGCA 
GCATCAGAAC TCATCTCCAG TGCATCACTC TCTGGTAATT CATCTTTTGC AGCTGCATAT 
CTCTATTATG ATCTCTGTGA AAGGCTCATA TCATTTGGAA AACTTTCTGA GGCTCTTTCA 
TATGCAAAAG AAGCCTATAG GATAAGGACT CTTATATTTC AAGATAAATT TAAGTATACA 
GCTGAGAAGC ATATCGAAAA ACACAATGAA GATGGGAAAA TATCAGAAAT ACGGACTTTT 
AGCATAAAAA ATTTCCAAGT CTACAGATTG TTAGCAACTG ATTTTTGGCC ATGTGGAAAT 
TTTCTCTGGG ACATCAATCG CTGCTACCTA AGTCCTTGGA GTGTACTCCA ATGTTATTTG 
GAAAGCACCC TTCAGGTTGG AATTCTGAAT GAGCTCATAG GGAATGGACT GGAGGCAGAA 
ACCATTCTAT CATGGGGGAA AGCCTTTTCA TGCTCGCAAA GTCTATTCCC TTTTGTAGTT 
GCATTTTCTT CTGCCTTAGG AAATCTATAC CACAAAAAGC AGTGTCTGGA TCTGGCAGAA 
AAGGAACTCC AAAATGCAAA AGAGATATTA ATTGCTAATC AGCGAGATTT TTCTTGCGTA 
AAGTGCAAAC TGAAGTTGGA AGTTACATTG GATAAACAAC TTGGAGACAT ATCTCGGAAA 
CAAATTGATA GAGTTTCACA GACAGATGGA TTCTTGCATG CTGAAAGCTT GTTTAGTGCT 
GCCCTGGGAA AATTCTGTTG CTCAGCATGG AAAAGCTGCA TTAGATCACA TGGGGAAGAA 
ATTGCTGAGG AGATAGTGAT TGATAGAAAT GGAGGGGAAG GTTTAGGACA TAATTCAAGT 
AAAACAAAGC TCAGTATAAA GGAGCCACCA GGAAACAGAG GCTCCAGGAG GGGTGGAAGA 
GCTAACAAAA CTTGTTTGTC AAAGGATCAG GATTTGATAT CTGAGCCAAC ATCAAGGTTG 
ACTCGATCTA TGCGTCACTC ACTTAGAGAG CAATGCCAAA ACCGTTCTAA TGTGCCTGAA 
GTTGTTTCAA AGAAGCCTAA TTTATGTGAT AGATCTGTTG GTTCTAGGGG TGAAAGGGTT 
TTGTTGGACA CGAGTAATGC ACTCCCTGGC TTTTGTATTT GCTACAAAGA AAAACGTCAG 
CAGTGCCTCT CGGAAGAAGT AACAGAATCT GGGTCTCTCA ACAATTTAGT AAGTTTGAAA 
TGGGAACTCT GCCATAGGAA GCTTGCGTCC TCAATACTTG TTAGCCTTGG AAAATGTTTG 
GGAGACTCTG GTAGAATTCA TCTAGCTCAT GAGGCTTTAC TGCATAGTAT CTCTGTTTTA 
TTCAAAAGCA CCTGGTCCAG CCACAACCAG CCTTCTGTCA GTCAGTTGCT GGAATTTATC 
GGGAAAGAAG TTACAAGAGA TGTATTTGCC GTTGATCGTG CAATAATATT ATACAACTTG 
TGCTGGTTGA ATTTGCGGAA TTACCACTGC AGGAAGAGTA GGTCTATCTG CTGTGATCTG 
TTTCATATCC CTTTCACAAA ACTAGTGTCA TGGTTAATGT TAGCATTTGT ACTCAGCGGA 
GAGGTTCCAA TATTATTTCA AAAGGTTTCA AGATTGCTTG CCTCTTTATA TCTGCTTTCT 
TCCTCAAATT CTGAATTCAC ATTCGAATCT GATGGAAATG AGCTGTCAGC AAGTCATTGG 
GTCTCATTTT TCCATCAAGC CTCGCTTGGT ACCCATCTCA GTTACCATTT CATTTCAAAT 
TTGAGCCAAA AACACAAATC ACAGTGCCTC TCAGATAAAG AGTGCACAGA GGCTACTTGC 
TCGAGTTGCA TGGTTCCTGA GGATTTGGAC TTACCCAGGC TTGCTCCTGA CCGTACTCAA 
GATCTTGTAC AATTCGCTAA AGAATTTTTC ATCAATCTTC CAAGCTCAAC AATCATCTGC 
ATTAGTTTGC TTGGAGGTGC TTTAAACCAA TTGTTACAGG AGCTAATGCA TATTCGTTCA 
CCTGTTTGTG CCTGGGTGCT CATATCACGC CTAAACCCAG AAAGTCAACC GGTTGCCACA 
CTTTTACCTG TAGATTCGAT TGTAGAAGAC ATGTCAGATG ACAGTGCAAA TCTTAGCTCT 
ACCGAAGCGA CTCAAGTCAA GAGTTTAAAG GGGCCCTGGC TTTGTCCATG GGGTACCACC 
GTGGTTGATG AGGTAGCTCC AGCATTTAAA TCAATATTGG AGGAAAGCCA CTCATCTTCT 
TCTACTACTG AAGAAGACAC AATAGAGAGT AGGGGCCTGT GGTGGAAAAA GAGAAAGAAA 
CTTAATCATC GTCTGGGAAT ATTCCTAAGG AATCTAGAAG CTTCCTGGCT GGGTCCCTGG 
AGGTGTCTGC TTCTCGGAGA ATGGTCGAAC TACAAATTGC CTGACTCAGC GCAGAAAAAG 
CTGGTAAATG ATCTCAAGTC CAAGTGCAAA ATGGAAGTAA ATGAGATGCT TTTGAAGGTT 
ATTCTTGGAG GTGGGACGGA TAACTTTAAA GGAGAAGCAT GCGTTGCTCA GCTTTCTTTA 
AGAAATGGTT GCTATGTAGG TAGAGGGGGA TATCTTTATG AAGAAGATAG CTGTAAAACT 
CCTACTGCTG CGTCTAATAT ATCTGAAAGT AGGCATGAAT TGGCCTTAAA ACTGATACAT 
GACGCAGCAA GCAAACTAGG GCAACAAGAT GGACATGAAA ATAGAGAACC TATCATTCTT 
GTGTTAGATC CCGAGGTTCA GATGCTTCCT TGGGAGAATA TTCCAATACT AAGGAAACAG 
GAGGTGTACC GCATGCCTTC CGTAGGTTGC ATATCTGCCG TGCTAAAGAA GCGCAGTCTT 
CAAGGAGAGC CAGCAAAATC TCATGTTGCT TCCTTCCCTC TTATTGATCC GCTAGATTCA 
TTTTACTTGT TGAATCCTGG TGGTGATCTC ACCGATACAC AAGTCACGTT TGAGAGTTGG 
TTCAGAGATC AAAACTTTGA GGGTAAAGCT GGGTCAGAAC CAAGTGCTAT AGAGTTGACA 
GAAGCATTAG AAACTCATGA TCTCTTTCTA TACTTTGGTC ATGGAAGCGG AGCACAGTAT 
ATACCTAGGC GTGAAATAGA GAAGCTAGAT AACTGTTCTG CAACTTTCTT AATGGGATGC 
AGTAGTGGTT CACTATGGCT AAAAGGCTGC TACATCCCAC AAGGTGTACC ACTTTCTTAT 
CTCCTAGGTG GATCTCCAGC CATTGTGGCG ACTCTATGGG ACGTAACAGA CCGAGACATT 
GATCGGTTTG GGAAAGCGTT GTTGGAAGCA TGGTTGCAAG AGAGGTCAGA TTCTTCTTCA 
GAGGGTGGCT GCAGCCAATG TGAGTCATTA GCTAATGACC TTGCAGCAAT GACTCTAAAA 
GGCACCAAGA GGTCAAGGAA ACCATCATCG CGTAACAAGC CAGCTCAATC AGATGTGGAT 
GGGTCAGGGA AGATTGAGTG CAACCACAAG CATAGACGTA AAATCGGTTC ATTCATAGCC 
GCGGCTCGAG ATGCGTGCAA CTTGCAATAC TTGATTGGGG CTGCGCCTGT CTGTTATGGT 
GTGCCAACTG GTATCACTAG GAAAAAAGGA ATTGATGCTC TTCTTCCTTC TTCATCTCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194028.2
CDS	99..6641
Translation	MASSGDDLRLLSLIDVGDNVFSSFSDYLKPFSTLSTSRKKQDRATTIRALAKQFLPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQRLRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPEVQDGDAEMALVVVDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAVSLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMSVTIDISMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVEAIPQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVDSYSGDEGNQTGSSSIGGHRNYNNKTHDSCKDRNKITCWPQYVDALKFLCQPLADFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLFLQRCTSDKGDREIANNKAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYRNKELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYLDILQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCTTLYSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDMHIERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYCLRAFCTQEADPNSKKVFQDISTSLNLWLRILSLDDSGDSLPTENIIPLLYNMIDLMSVKGCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSENCADKSTCIDFWMDCLKDSKAKLIGFQQNFHDLHNKDEGPFQSDITIDDIKDAASELISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTAEKHIEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTLQVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQNAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRVSQTDGFLHAESLFSAALGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRANKTCLSKDQDLISEPTSRLTRSMRHSLREQCQNRSNVPEVVSKKPNLCDRSVGSRGERVLLDTSNALPGFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASSILVSLGKCLGDSGRIHLAHEALLHSISVLFKSTWSSHNQPSVSQLLEFIGKEVTRDVFAVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKVSRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQCLSDKECTEATCSSCMVPEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIICISLLGGALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDMSDDSANLSSTEATQVKSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSSTTEEDTIESRGLWWKKRKKLNHRLGIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLKVILGGGTDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLIHDAASKLGQQDGHENREPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKKRSLQGEPAKSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAIELTEALETHDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVPLSYLLGGSPAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAMTLKGTKRSRKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPVCYGVPTGITRKKGIDALLPSSSR

Target ORF information:

RefSeq Version	NM_118426.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana separase (ESP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118426.4

ORF Insert Sequence:

ATGGCGTCCT CTGGCGACGA CCTCCGTCTT CTCTCTCTCA TCGACGTCGG CGATAATGTC 
TTCTCTTCCT TCTCCGATTA CCTTAAACCT TTCTCTACTC TATCCACTTC CCGGAAAAAA 
CAAGACCGAG CAACCACGAT TCGAGCCCTA GCTAAGCAAT TCCTTCCTTT CCTCAACAAA 
TCGATCTCTC TCCTACCGAA ACGTCTCTCC GTCGCGAATT CTGATAAGGA AGCTCGTGAA 
TCAGCATTAG ATCTGTTCCG TGCTTATGAA CTTTGTTTGG ACTGTTTAGA ATTGGTCTCT 
GCTCAATTAG CTTGTAAGCC TCACACTGTT CAATCTCAGA GGCTAAGGAT GATTCATTGT 
CTCGATGTTT GGGGTTTGTA TGAGAATGTG TATACTGAAG CGTTTAAGGT TCTGGAGAAG 
CTAAGAGGCT CTGATAGCAA ATCCCGGAAG TCCCGGTTGT TGCCGGAGGT TCAGGATGGA 
GATGCGGAGA TGGCTTTAGT TGTTGTTGAT GCTGTGGCGG CCATCTTTAG GGCTGTGGCA 
ATGAGTCAGC AGTTAGATGA TAAGCGGTAT CGGAAAGTTC TTCTTTTGCT TGAGGAAGTC 
GGAGGCTGGT TAAGGGTCTT AGATGCTAAG GTGTATGAAA AGTTGCACAG AGCGATGGTG 
ACAAGTATGG GTAAATGTGC TGTTTCTCTC GTTAGAGAAG CTGAGCGTTT CAATGGGGAT 
TTAGTGATTT CTTTCTGCGA CTTAACTGTG AAGGAACATT ATAAATCTGC ATTATCAAAA 
GATCGAGTTT ATAAGTTTGC TCGCGAGGTG CTTTCTGTTC TGTTTGGATT TAAGGATAGA 
AAAATGTCAG TGACCATTGA CATTTCAATG TCTGTGTTGC GCAGCTTATC TTGCCAATTT 
GAGGATGAAA GTAATGAGAA TTTAATGGAG TTTTTTGACC TAGTCGATTA TTGCGCTCAC 
AAGTTTCGAG CAGCAGGAGA CATGTACTGT GCAAAAGTTT CAAAAAAATT GAATGAGATG 
GCAGCTATTT TCGTAGAGGC AATACCCCAA CTTAATTTGG TTCTTAGACT GTATTCGACC 
GGGCTGTCCA TCACGGTTTG CAATTCCAAG CTCGGAGAAA TTAAGCTAGA AGATTCAACA 
GATGATTGGA AGATTCAAGC TATGTTTGAT GATGATGCTA GATGGCAAAG CTTAGTTTCT 
TTACTCGGCA TGGTTGATAG TTACTCAGGT GATGAGGGCA ATCAGACTGG TTCATCATCG 
ATTGGTGGGC ATAGGAACTA CAACAATAAA ACACATGATA GCTGTAAAGA CAGGAACAAG 
ATTACTTGTT GGCCACAATA CGTAGATGCC TTGAAATTCT TGTGCCAACC GCTTGCAGAC 
TTTATATATT CTGTGAAAAG AAAGATTGTG CTGGAGACAG AAATGTCATG CGCCTCTGCT 
CATCTAATTA CTATTCACGA TGCCTTTCTC CAGTTTTGTG ATGGCTGTCT CTTCCTTCAG 
AGGTGCACGT CTGACAAGGG AGATAGAGAA ATTGCCAATA ACAAAGCTTT CTTAAACGCG 
GCTATGGGTG CTTTCATTGT CTCATTGAGA ACCCAACTTA AATTGGAGAT AAGTGCCCAT 
CTAGTTGAGG ATGTAATTGG AAGCCCATGG ATCCAATCTC AAGAACTCAA GTATCTAATT 
GCGACGCTGT ACAATATTGG TATCGTTTTG TACAGAAATA AGGAGCTAAA CAAGGCTTGT 
GAGGCGCTTA AGCTGTGCTC CAAGGTATCA TGGAGATGTG TTGAACTACA TTGTCATATG 
TTTGTAAATC AGTCTAGTTC ATCTGATAAT GACTTGTCAG AAGATGCCAT TATGGATTTT 
GTGGGCGAAG CATGTAATAG GTGTGCATTC TACCTGGATA TACTCCAGAA ATGTAGCAGA 
CGTAAGATTA GACAGAATAT TGTTCATATT CTAGAAAATT GGTTGTCAGC TGAACATCTG 
ATTAGACGGT TACCAGGCCC TGAGGCAATT GTAAAGCAAT GGGTTAAGAT AGAGCGGGAA 
TGTCACACGG ATCTGGATGC GGCAGGTTCT TGTACAACTT TGTACTCATT GTTATCATCT 
TCTCAAAAAA AGTCAAAGCG GGGTATCGGA AAAATCCTTG AGCAGGAGCT TCTTGCCTAT 
GACAGAGTGT TACCTTTAAG GTCAAATCTA GGTCAACAAA CGCGAATCAA GATAGCAGAT 
ATCCTCTTGA AGGATGTTTA TGTCACTGAG GATATGCATA TAGAGAGAGC AAGAATTTTG 
ATATGGAAAG CACGAATGAC AAGGACATCT GGAACTGAAC ATATAACCGA GTGCATTTGT 
TTTCTGTCTG AAGCAATCTC TATATTGGGT GAGTTACATC ATGGGCCAAA TGAAGAGGGT 
TCTCCATCTT CTCACATGTT ACCTATTGCG TATTGCTTGA GAGCTTTTTG TACCCAGGAA 
GCTGACCCAA ACTCGAAGAA AGTCTTTCAA GATATTAGCA CTTCACTAAA TCTCTGGCTA 
AGGATTCTTA GCCTAGATGA TAGTGGAGAC AGTCTACCAA CAGAGAATAT AATTCCGTTA 
CTGTATAATA TGATTGATTT GATGTCAGTG AAGGGATGTA CAGAGCTCCA TCATCATATA 
TACCAACTGA TTTTCAGATT GTTTAAATGG AAGAATGTCA AATTGGAGGT CTGCCTTGCA 
ATGCTGTGGG AATGTAGAAG GCTTAGTCAT GCCTTATGCC CTTCCCCCAT AAGTGATGCC 
TTTATCCAGA CCTTATCAGA GAATTGTGCT GACAAATCCA CATGCATTGA TTTCTGGATG 
GATTGCCTGA AAGATTCAAA AGCAAAGTTA ATTGGGTTCC AGCAGAACTT CCATGATTTA 
CATAATAAGG ATGAAGGTCC CTTTCAATCA GACATCACAA TTGACGATAT TAAAGATGCA 
GCATCAGAAC TCATCTCCAG TGCATCACTC TCTGGTAATT CATCTTTTGC AGCTGCATAT 
CTCTATTATG ATCTCTGTGA AAGGCTCATA TCATTTGGAA AACTTTCTGA GGCTCTTTCA 
TATGCAAAAG AAGCCTATAG GATAAGGACT CTTATATTTC AAGATAAATT TAAGTATACA 
GCTGAGAAGC ATATCGAAAA ACACAATGAA GATGGGAAAA TATCAGAAAT ACGGACTTTT 
AGCATAAAAA ATTTCCAAGT CTACAGATTG TTAGCAACTG ATTTTTGGCC ATGTGGAAAT 
TTTCTCTGGG ACATCAATCG CTGCTACCTA AGTCCTTGGA GTGTACTCCA ATGTTATTTG 
GAAAGCACCC TTCAGGTTGG AATTCTGAAT GAGCTCATAG GGAATGGACT GGAGGCAGAA 
ACCATTCTAT CATGGGGGAA AGCCTTTTCA TGCTCGCAAA GTCTATTCCC TTTTGTAGTT 
GCATTTTCTT CTGCCTTAGG AAATCTATAC CACAAAAAGC AGTGTCTGGA TCTGGCAGAA 
AAGGAACTCC AAAATGCAAA AGAGATATTA ATTGCTAATC AGCGAGATTT TTCTTGCGTA 
AAGTGCAAAC TGAAGTTGGA AGTTACATTG GATAAACAAC TTGGAGACAT ATCTCGGAAA 
CAAATTGATA GAGTTTCACA GACAGATGGA TTCTTGCATG CTGAAAGCTT GTTTAGTGCT 
GCCCTGGGAA AATTCTGTTG CTCAGCATGG AAAAGCTGCA TTAGATCACA TGGGGAAGAA 
ATTGCTGAGG AGATAGTGAT TGATAGAAAT GGAGGGGAAG GTTTAGGACA TAATTCAAGT 
AAAACAAAGC TCAGTATAAA GGAGCCACCA GGAAACAGAG GCTCCAGGAG GGGTGGAAGA 
GCTAACAAAA CTTGTTTGTC AAAGGATCAG GATTTGATAT CTGAGCCAAC ATCAAGGTTG 
ACTCGATCTA TGCGTCACTC ACTTAGAGAG CAATGCCAAA ACCGTTCTAA TGTGCCTGAA 
GTTGTTTCAA AGAAGCCTAA TTTATGTGAT AGATCTGTTG GTTCTAGGGG TGAAAGGGTT 
TTGTTGGACA CGAGTAATGC ACTCCCTGGC TTTTGTATTT GCTACAAAGA AAAACGTCAG 
CAGTGCCTCT CGGAAGAAGT AACAGAATCT GGGTCTCTCA ACAATTTAGT AAGTTTGAAA 
TGGGAACTCT GCCATAGGAA GCTTGCGTCC TCAATACTTG TTAGCCTTGG AAAATGTTTG 
GGAGACTCTG GTAGAATTCA TCTAGCTCAT GAGGCTTTAC TGCATAGTAT CTCTGTTTTA 
TTCAAAAGCA CCTGGTCCAG CCACAACCAG CCTTCTGTCA GTCAGTTGCT GGAATTTATC 
GGGAAAGAAG TTACAAGAGA TGTATTTGCC GTTGATCGTG CAATAATATT ATACAACTTG 
TGCTGGTTGA ATTTGCGGAA TTACCACTGC AGGAAGAGTA GGTCTATCTG CTGTGATCTG 
TTTCATATCC CTTTCACAAA ACTAGTGTCA TGGTTAATGT TAGCATTTGT ACTCAGCGGA 
GAGGTTCCAA TATTATTTCA AAAGGTTTCA AGATTGCTTG CCTCTTTATA TCTGCTTTCT 
TCCTCAAATT CTGAATTCAC ATTCGAATCT GATGGAAATG AGCTGTCAGC AAGTCATTGG 
GTCTCATTTT TCCATCAAGC CTCGCTTGGT ACCCATCTCA GTTACCATTT CATTTCAAAT 
TTGAGCCAAA AACACAAATC ACAGTGCCTC TCAGATAAAG AGTGCACAGA GGCTACTTGC 
TCGAGTTGCA TGGTTCCTGA GGATTTGGAC TTACCCAGGC TTGCTCCTGA CCGTACTCAA 
GATCTTGTAC AATTCGCTAA AGAATTTTTC ATCAATCTTC CAAGCTCAAC AATCATCTGC 
ATTAGTTTGC TTGGAGGTGC TTTAAACCAA TTGTTACAGG AGCTAATGCA TATTCGTTCA 
CCTGTTTGTG CCTGGGTGCT CATATCACGC CTAAACCCAG AAAGTCAACC GGTTGCCACA 
CTTTTACCTG TAGATTCGAT TGTAGAAGAC ATGTCAGATG ACAGTGCAAA TCTTAGCTCT 
ACCGAAGCGA CTCAAGTCAA GAGTTTAAAG GGGCCCTGGC TTTGTCCATG GGGTACCACC 
GTGGTTGATG AGGTAGCTCC AGCATTTAAA TCAATATTGG AGGAAAGCCA CTCATCTTCT 
TCTACTACTG AAGAAGACAC AATAGAGAGT AGGGGCCTGT GGTGGAAAAA GAGAAAGAAA 
CTTAATCATC GTCTGGGAAT ATTCCTAAGG AATCTAGAAG CTTCCTGGCT GGGTCCCTGG 
AGGTGTCTGC TTCTCGGAGA ATGGTCGAAC TACAAATTGC CTGACTCAGC GCAGAAAAAG 
CTGGTAAATG ATCTCAAGTC CAAGTGCAAA ATGGAAGTAA ATGAGATGCT TTTGAAGGTT 
ATTCTTGGAG GTGGGACGGA TAACTTTAAA GGAGAAGCAT GCGTTGCTCA GCTTTCTTTA 
AGAAATGGTT GCTATGTAGG TAGAGGGGGA TATCTTTATG AAGAAGATAG CTGTAAAACT 
CCTACTGCTG CGTCTAATAT ATCTGAAAGT AGGCATGAAT TGGCCTTAAA ACTGATACAT 
GACGCAGCAA GCAAACTAGG GCAACAAGAT GGACATGAAA ATAGAGAACC TATCATTCTT 
GTGTTAGATC CCGAGGTTCA GATGCTTCCT TGGGAGAATA TTCCAATACT AAGGAAACAG 
GAGGTGTACC GCATGCCTTC CGTAGGTTGC ATATCTGCCG TGCTAAAGAA GCGCAGTCTT 
CAAGGAGAGC CAGCAAAATC TCATGTTGCT TCCTTCCCTC TTATTGATCC GCTAGATTCA 
TTTTACTTGT TGAATCCTGG TGGTGATCTC ACCGATACAC AAGTCACGTT TGAGAGTTGG 
TTCAGAGATC AAAACTTTGA GGGTAAAGCT GGGTCAGAAC CAAGTGCTAT AGAGTTGACA 
GAAGCATTAG AAACTCATGA TCTCTTTCTA TACTTTGGTC ATGGAAGCGG AGCACAGTAT 
ATACCTAGGC GTGAAATAGA GAAGCTAGAT AACTGTTCTG CAACTTTCTT AATGGGATGC 
AGTAGTGGTT CACTATGGCT AAAAGGCTGC TACATCCCAC AAGGTGTACC ACTTTCTTAT 
CTCCTAGGTG GATCTCCAGC CATTGTGGCG ACTCTATGGG ACGTAACAGA CCGAGACATT 
GATCGGTTTG GGAAAGCGTT GTTGGAAGCA TGGTTGCAAG AGAGGTCAGA TTCTTCTTCA 
GAGGGTGGCT GCAGCCAATG TGAGTCATTA GCTAATGACC TTGCAGCAAT GACTCTAAAA 
GGCACCAAGA GGTCAAGGAA ACCATCATCG CGTAACAAGC CAGCTCAATC AGATGTGGAT 
GGGTCAGGGA AGATTGAGTG CAACCACAAG CATAGACGTA AAATCGGTTC ATTCATAGCC 
GCGGCTCGAG ATGCGTGCAA CTTGCAATAC TTGATTGGGG CTGCGCCTGT CTGTTATGGT 
GTGCCAACTG GTATCACTAG GAAAAAAGGA ATTGATGCTC TTCTTCCTTC TTCATCTCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESP ( NM_001203875.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190804.1 Arabidopsis thaliana separase (ESP), mRNA.

Price & Availability↑

CloneID	OAb30163
Clone ID Related Accession (Same CDS sequence)	NM_001203875.2
Accession Version	NM_001203875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	separase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_001203875.1.

ATGGCGTCCT CTGGCGACGA CCTCCGTCTT CTCTCTCTCA TCGACGTCGG CGATAATGTC 
TTCTCTTCCT TCTCCGATTA CCTTAAACCT TTCTCTACTC TATCCACTTC CCGGAAAAAA 
CAAGACCGAG CAACCACGAT TCGAGCCCTA GCTAAGCAAT TCCTTCCTTT CCTCAACAAA 
TCGATCTCTC TCCTACCGAA ACGTCTCTCC GTCGCGAATT CTGATAAGGA AGCTCGTGAA 
TCAGCATTAG ATCTGTTCCG TGCTTATGAA CTTTGTTTGG ACTGTTTAGA ATTGGTCTCT 
GCTCAATTAG CTTGTAAGCC TCACACTGTT CAATCTCAGA GGCTAAGGAT GATTCATTGT 
CTCGATGTTT GGGGTTTGTA TGAGAATGTG TATACTGAAG CGTTTAAGGT TCTGGAGAAG 
CTAAGAGGCT CTGATAGCAA ATCCCGGAAG TCCCGGTTGT TGCCGGAGGT TCAGGATGGA 
GATGCGGAGA TGGCTTTAGT TGTTGTTGAT GCTGTGGCGG CCATCTTTAG GGCTGTGGCA 
ATGAGTCAGC AGTTAGATGA TAAGCGGTAT CGGAAAGTTC TTCTTTTGCT TGAGGAAGTC 
GGAGGCTGGT TAAGGGTCTT AGATGCTAAG GTGTATGAAA AGTTGCACAG AGCGATGGTG 
ACAAGTATGG GTAAATGTGC TGTTTCTCTC GTTAGAGAAG CTGAGCGTTT CAATGGGGAT 
TTAGTGATTT CTTTCTGCGA CTTAACTGTG AAGGAACATT ATAAATCTGC ATTATCAAAA 
GATCGAGTTT ATAAGTTTGC TCGCGAGGTG CTTTCTGTTC TGTTTGGATT TAAGGATAGA 
AAAATGTCAG TGACCATTGA CATTTCAATG TCTGTGTTGC GCAGCTTATC TTGCCAATTT 
GAGGATGAAA GTAATGAGAA TTTAATGGAG TTTTTTGACC TAGTCGATTA TTGCGCTCAC 
AAGTTTCGAG CAGCAGGAGA CATGTACTGT GCAAAAGTTT CAAAAAAATT GAATGAGATG 
GCAGCTATTT TCGTAGAGGC AATACCCCAA CTTAATTTGG TTCTTAGACT GTATTCGACC 
GGGCTGTCCA TCACGGTTTG CAATTCCAAG CTCGGAGAAA TTAAGCTAGA AGATTCAACA 
GATGATTGGA AGATTCAAGC TATGTTTGAT GATGATGCTA GATGGCAAAG CTTAGTTTCT 
TTACTCGGCA TGGTTGATAG TTACTCAGGT GATGAGGGCA ATCAGACTGG TTCATCATCG 
ATTGGTGGGC ATAGGAACTA CAACAATAAA ACACATGATA GCTGTAAAGA CAGGAACAAG 
ATTACTTGTT GGCCACAATA CGTAGATGCC TTGAAATTCT TGTGCCAACC GCTTGCAGAC 
TTTATATATT CTGTGAAAAG AAAGATTGTG CTGGAGACAG AAATGTCATG CGCCTCTGCT 
CATCTAATTA CTATTCACGA TGCCTTTCTC CAGTTTTGTG ATGGCTGTCT CTTCCTTCAG 
AGGTGCACGT CTGACAAGGG AGATAGAGAA ATTGCCAATA ACAAAGCTTT CTTAAACGCG 
GCTATGGGTG CTTTCATTGT CTCATTGAGA ACCCAACTTA AATTGGAGAT AAGTGCCCAT 
CTAGTTGAGG ATGTAATTGG AAGCCCATGG ATCCAATCTC AAGAACTCAA GTATCTAATT 
GCGACGCTGT ACAATATTGG TATCGTTTTG TACAGAAATA AGGAGCTAAA CAAGGCTTGT 
GAGGCGCTTA AGCTGTGCTC CAAGGTATCA TGGAGATGTG TTGAACTACA TTGTCATATG 
TTTGTAAATC AGTCTAGTTC ATCTGATAAT GACTTGTCAG AAGATGCCAT TATGGATTTT 
GTGGGCGAAG CATGTAATAG GTGTGCATTC TACCTGGATA TACTCCAGAA ATGTAGCAGA 
CGTAAGATTA GACAGAATAT TGTTCATATT CTAGAAAATT GGTTGTCAGC TGAACATCTG 
ATTAGACGGT TACCAGGCCC TGAGGCAATT GTAAAGCAAT GGGTTAAGAT AGAGCGGGAA 
TGTCACACGG ATCTGGATGC GGCAGGTTCT TGTACAACTT TGTACTCATT GTTATCATCT 
TCTCAAAAAA AGTCAAAGCG GGGTATCGGA AAAATCCTTG AGCAGGAGCT TCTTGCCTAT 
GACAGAGTGT TACCTTTAAG GTCAAATCTA GGTCAACAAA CGCGAATCAA GATAGCAGAT 
ATCCTCTTGA AGGATGTTTA TGTCACTGAG GATATGCATA TAGAGAGAGC AAGAATTTTG 
ATATGGAAAG CACGAATGAC AAGGACATCT GGAACTGAAC ATATAACCGA GTGCATTTGT 
TTTCTGTCTG AAGCAATCTC TATATTGGGT GAGTTACATC ATGGGCCAAA TGAAGAGGGT 
TCTCCATCTT CTCACATGTT ACCTATTGCG TATTGCTTGA GAGCTTTTTG TACCCAGGAA 
GCTGACCCAA ACTCGAAGAA AGTCTTTCAA GATATTAGCA CTTCACTAAA TCTCTGGCTA 
AGGATTCTTA GCCTAGATGA TAGTGGAGAC AGTCTACCAA CAGAGAATAT AATTCCGTTA 
CTGTATAATA TGATTGATTT GATGTCAGTG AAGGGATGTA CAGAGCTCCA TCATCATATA 
TACCAACTGA TTTTCAGATT GTTTAAATGG AAGAATGTCA AATTGGAGGT CTGCCTTGCA 
ATGCTGTGGG AATGTAGAAG GCTTAGTCAT GCCTTATGCC CTTCCCCCAT AAGTGATGCC 
TTTATCCAGA CCTTATCAGA GAATTGTGCT GACAAATCCA CATGCATTGA TTTCTGGATG 
GATTGCCTGA AAGATTCAAA AGCAAAGTTA ATTGGGTTCC AGCAGAACTT CCATGATTTA 
CATAATAAGG ATGAAGGTCC CTTTCAATCA GACATCACAA TTGACGATAT TAAAGATGCA 
GCATCAGAAC TCATCTCCAG TGCATCACTC TCTGGTAATT CATCTTTTGC AGCTGCATAT 
CTCTATTATG ATCTCTGTGA AAGGCTCATA TCATTTGGAA AACTTTCTGA GGCTCTTTCA 
TATGCAAAAG AAGCCTATAG GATAAGGACT CTTATATTTC AAGATAAATT TAAGTATACA 
GCTGAGAAGC ATATCGAAAA ACACAATGAA GATGGGAAAA TATCAGAAAT ACGGACTTTT 
AGCATAAAAA ATTTCCAAGT CTACAGATTG TTAGCAACTG ATTTTTGGCC ATGTGGAAAT 
TTTCTCTGGG ACATCAATCG CTGCTACCTA AGTCCTTGGA GTGTACTCCA ATGTTATTTG 
GAAAGCACCC TTCAGGTTGG AATTCTGAAT GAGCTCATAG GGAATGGACT GGAGGCAGAA 
ACCATTCTAT CATGGGGGAA AGCCTTTTCA TGCTCGCAAA GTCTATTCCC TTTTGTAGTT 
GCATTTTCTT CTGCCTTAGG AAATCTATAC CACAAAAAGC AGTGTCTGGA TCTGGCAGAA 
AAGGAACTCC AAAATGCAAA AGAGATATTA ATTGCTAATC AGCGAGATTT TTCTTGCGTA 
AAGTGCAAAC TGAAGTTGGA AGTTACATTG GATAAACAAC TTGGAGACAT ATCTCGGAAA 
CAAATTGATA GAGTTTCACA GACAGATGGA TTCTTGCATG CTGAAAGCTT GTTTAGTGCT 
GCCCTGGGAA AATTCTGTTG CTCAGCATGG AAAAGCTGCA TTAGATCACA TGGGGAAGAA 
ATTGCTGAGG AGATAGTGAT TGATAGAAAT GGAGGGGAAG GTTTAGGACA TAATTCAAGT 
AAAACAAAGC TCAGTATAAA GGAGCCACCA GGAAACAGAG GCTCCAGGAG GGGTGGAAGA 
GCTAACAAAA CTTGTTTGTC AAAGGATCAG GATTTGATAT CTGAGCCAAC ATCAAGGTTG 
ACTCGATCTA TGCGTCACTC ACTTAGAGAG CAATGCCAAA ACCGTTCTAA TGTGCCTGAA 
GTTGTTTCAA AGAAGCCTAA TTTATGTGAT AGATCTGTTG GTTCTAGGGG TGAAAGGGTT 
TTGTTGGACA CGAGTAATGC ACTCCCTGGC TTTTGTATTT GCTACAAAGA AAAACGTCAG 
CAGTGCCTCT CGGAAGAAGT AACAGAATCT GGGTCTCTCA ACAATTTAGT AAGTTTGAAA 
TGGGAACTCT GCCATAGGAA GCTTGCGTCC TCAATACTTG TTAGCCTTGG AAAATGTTTG 
GGAGACTCTG GTAGAATTCA TCTAGCTCAT GAGGCTTTAC TGCATAGTAT CTCTGTTTTA 
TTCAAAAGCA CCTGGTCCAG CCACAACCAG CCTTCTGTCA GTCAGTTGCT GGAATTTATC 
GGGAAAGAAG TTACAAGAGA TGTATTTGCC GTTGATCGTG CAATAATATT ATACAACTTG 
TGCTGGTTGA ATTTGCGGAA TTACCACTGC AGGAAGAGTA GGTCTATCTG CTGTGATCTG 
TTTCATATCC CTTTCACAAA ACTAGTGTCA TGGTTAATGT TAGCATTTGT ACTCAGCGGA 
GAGGTTCCAA TATTATTTCA AAAGGTTTCA AGATTGCTTG CCTCTTTATA TCTGCTTTCT 
TCCTCAAATT CTGAATTCAC ATTCGAATCT GATGGAAATG AGCTGTCAGC AAGTCATTGG 
GTCTCATTTT TCCATCAAGC CTCGCTTGGT ACCCATCTCA GTTACCATTT CATTTCAAAT 
TTGAGCCAAA AACACAAATC ACAGTGCCTC TCAGATAAAG AGTGCACAGA GGCTACTTGC 
TCGAGTTGCA TGGTTCCTGA GGATTTGGAC TTACCCAGGC TTGCTCCTGA CCGTACTCAA 
GATCTTGTAC AATTCGCTAA AGAATTTTTC ATCAATCTTC CAAGCTCAAC AATCATCTGC 
ATTAGTTTGC TTGGAGGTGC TTTAAACCAA TTGTTACAGG AGCTAATGCA TATTCGTTCA 
CCTGTTTGTG CCTGGGTGCT CATATCACGC CTAAACCCAG AAAGTCAACC GGTTGCCACA 
CTTTTACCTG TAGATTCGAT TGTAGAAGAT GACAGTGCAA ATCTTAGCTC TACCGAAGCG 
ACTCAAGTCA AGAGTTTAAA GGGGCCCTGG CTTTGTCCAT GGGGTACCAC CGTGGTTGAT 
GAGGTAGCTC CAGCATTTAA ATCAATATTG GAGGAAAGCC ACTCATCTTC TTCTACTACT 
GAAGAAGACA CAATAGAGAG TAGGGGCCTG TGGTGGAAAA AGAGAAAGAA ACTTAATCAT 
CGTCTGGGAA TATTCCTAAG GAATCTAGAA GCTTCCTGGC TGGGTCCCTG GAGGTGTCTG 
CTTCTCGGAG AATGGTCGAA CTACAAATTG CCTGACTCAG CGCAGAAAAA GCTGGTAAAT 
GATCTCAAGT CCAAGTGCAA AATGGAAGTA AATGAGATGC TTTTGAAGGT TATTCTTGGA 
GGTGGGACGG ATAACTTTAA AGGAGAAGCA TGCGTTGCTC AGCTTTCTTT AAGAAATGGT 
TGCTATGTAG GTAGAGGGGG ATATCTTTAT GAAGAAGATA GCTGTAAAAC TCCTACTGCT 
GCGTCTAATA TATCTGAAAG TAGGCATGAA TTGGCCTTAA AACTGATACA TGACGCAGCA 
AGCAAACTAG GGCAACAAGA TGGACATGAA AATAGAGAAC CTATCATTCT TGTGTTAGAT 
CCCGAGGTTC AGATGCTTCC TTGGGAGAAT ATTCCAATAC TAAGGAAACA GGAGGTGTAC 
CGCATGCCTT CCGTAGGTTG CATATCTGCC GTGCTAAAGA AGCGCAGTCT TCAAGGAGAG 
CCAGCAAAAT CTCATGTTGC TTCCTTCCCT CTTATTGATC CGCTAGATTC ATTTTACTTG 
TTGAATCCTG GTGGTGATCT CACCGATACA CAAGTCACGT TTGAGAGTTG GTTCAGAGAT 
CAAAACTTTG AGGGTAAAGC TGGGTCAGAA CCAAGTGCTA TAGAGTTGAC AGAAGCATTA 
GAAACTCATG ATCTCTTTCT ATACTTTGGT CATGGAAGCG GAGCACAGTA TATACCTAGG 
CGTGAAATAG AGAAGCTAGA TAACTGTTCT GCAACTTTCT TAATGGGATG CAGTAGTGGT 
TCACTATGGC TAAAAGGCTG CTACATCCCA CAAGGTGTAC CACTTTCTTA TCTCCTAGGT 
GGATCTCCAG CCATTGTGGC GACTCTATGG GACGTAACAG ACCGAGACAT TGATCGGTTT 
GGGAAAGCGT TGTTGGAAGC ATGGTTGCAA GAGAGGTCAG ATTCTTCTTC AGAGGGTGGC 
TGCAGCCAAT GTGAGTCATT AGCTAATGAC CTTGCAGCAA TGACTCTAAA AGGCACCAAG 
AGGTCAAGGA AACCATCATC GCGTAACAAG CCAGCTCAAT CAGATGTGGA TGGGTCAGGG 
AAGATTGAGT GCAACCACAA GCATAGACGT AAAATCGGTT CATTCATAGC CGCGGCTCGA 
GATGCGTGCA ACTTGCAATA CTTGATTGGG GCTGCGCCTG TCTGTTATGG TGTGCCAACT 
GGTATCACTA GGAAAAAAGG AATTGATGCT CTTCTTCCTT CTTCATCTCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190804.1
CDS	157..6690
Translation	MASSGDDLRLLSLIDVGDNVFSSFSDYLKPFSTLSTSRKKQDRATTIRALAKQFLPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQRLRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPEVQDGDAEMALVVVDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAVSLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMSVTIDISMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVEAIPQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVDSYSGDEGNQTGSSSIGGHRNYNNKTHDSCKDRNKITCWPQYVDALKFLCQPLADFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLFLQRCTSDKGDREIANNKAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYRNKELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYLDILQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCTTLYSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDMHIERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYCLRAFCTQEADPNSKKVFQDISTSLNLWLRILSLDDSGDSLPTENIIPLLYNMIDLMSVKGCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSENCADKSTCIDFWMDCLKDSKAKLIGFQQNFHDLHNKDEGPFQSDITIDDIKDAASELISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTAEKHIEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTLQVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQNAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRVSQTDGFLHAESLFSAALGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRANKTCLSKDQDLISEPTSRLTRSMRHSLREQCQNRSNVPEVVSKKPNLCDRSVGSRGERVLLDTSNALPGFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASSILVSLGKCLGDSGRIHLAHEALLHSISVLFKSTWSSHNQPSVSQLLEFIGKEVTRDVFAVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKVSRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQCLSDKECTEATCSSCMVPEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIICISLLGGALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDDSANLSSTEATQVKSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSSTTEEDTIESRGLWWKKRKKLNHRLGIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLKVILGGGTDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLIHDAASKLGQQDGHENREPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKKRSLQGEPAKSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAIELTEALETHDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVPLSYLLGGSPAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAMTLKGTKRSRKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPVCYGVPTGITRKKGIDALLPSSSR

Target ORF information:

RefSeq Version	NM_001203875.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana separase (ESP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203875.2

ORF Insert Sequence:

ATGGCGTCCT CTGGCGACGA CCTCCGTCTT CTCTCTCTCA TCGACGTCGG CGATAATGTC 
TTCTCTTCCT TCTCCGATTA CCTTAAACCT TTCTCTACTC TATCCACTTC CCGGAAAAAA 
CAAGACCGAG CAACCACGAT TCGAGCCCTA GCTAAGCAAT TCCTTCCTTT CCTCAACAAA 
TCGATCTCTC TCCTACCGAA ACGTCTCTCC GTCGCGAATT CTGATAAGGA AGCTCGTGAA 
TCAGCATTAG ATCTGTTCCG TGCTTATGAA CTTTGTTTGG ACTGTTTAGA ATTGGTCTCT 
GCTCAATTAG CTTGTAAGCC TCACACTGTT CAATCTCAGA GGCTAAGGAT GATTCATTGT 
CTCGATGTTT GGGGTTTGTA TGAGAATGTG TATACTGAAG CGTTTAAGGT TCTGGAGAAG 
CTAAGAGGCT CTGATAGCAA ATCCCGGAAG TCCCGGTTGT TGCCGGAGGT TCAGGATGGA 
GATGCGGAGA TGGCTTTAGT TGTTGTTGAT GCTGTGGCGG CCATCTTTAG GGCTGTGGCA 
ATGAGTCAGC AGTTAGATGA TAAGCGGTAT CGGAAAGTTC TTCTTTTGCT TGAGGAAGTC 
GGAGGCTGGT TAAGGGTCTT AGATGCTAAG GTGTATGAAA AGTTGCACAG AGCGATGGTG 
ACAAGTATGG GTAAATGTGC TGTTTCTCTC GTTAGAGAAG CTGAGCGTTT CAATGGGGAT 
TTAGTGATTT CTTTCTGCGA CTTAACTGTG AAGGAACATT ATAAATCTGC ATTATCAAAA 
GATCGAGTTT ATAAGTTTGC TCGCGAGGTG CTTTCTGTTC TGTTTGGATT TAAGGATAGA 
AAAATGTCAG TGACCATTGA CATTTCAATG TCTGTGTTGC GCAGCTTATC TTGCCAATTT 
GAGGATGAAA GTAATGAGAA TTTAATGGAG TTTTTTGACC TAGTCGATTA TTGCGCTCAC 
AAGTTTCGAG CAGCAGGAGA CATGTACTGT GCAAAAGTTT CAAAAAAATT GAATGAGATG 
GCAGCTATTT TCGTAGAGGC AATACCCCAA CTTAATTTGG TTCTTAGACT GTATTCGACC 
GGGCTGTCCA TCACGGTTTG CAATTCCAAG CTCGGAGAAA TTAAGCTAGA AGATTCAACA 
GATGATTGGA AGATTCAAGC TATGTTTGAT GATGATGCTA GATGGCAAAG CTTAGTTTCT 
TTACTCGGCA TGGTTGATAG TTACTCAGGT GATGAGGGCA ATCAGACTGG TTCATCATCG 
ATTGGTGGGC ATAGGAACTA CAACAATAAA ACACATGATA GCTGTAAAGA CAGGAACAAG 
ATTACTTGTT GGCCACAATA CGTAGATGCC TTGAAATTCT TGTGCCAACC GCTTGCAGAC 
TTTATATATT CTGTGAAAAG AAAGATTGTG CTGGAGACAG AAATGTCATG CGCCTCTGCT 
CATCTAATTA CTATTCACGA TGCCTTTCTC CAGTTTTGTG ATGGCTGTCT CTTCCTTCAG 
AGGTGCACGT CTGACAAGGG AGATAGAGAA ATTGCCAATA ACAAAGCTTT CTTAAACGCG 
GCTATGGGTG CTTTCATTGT CTCATTGAGA ACCCAACTTA AATTGGAGAT AAGTGCCCAT 
CTAGTTGAGG ATGTAATTGG AAGCCCATGG ATCCAATCTC AAGAACTCAA GTATCTAATT 
GCGACGCTGT ACAATATTGG TATCGTTTTG TACAGAAATA AGGAGCTAAA CAAGGCTTGT 
GAGGCGCTTA AGCTGTGCTC CAAGGTATCA TGGAGATGTG TTGAACTACA TTGTCATATG 
TTTGTAAATC AGTCTAGTTC ATCTGATAAT GACTTGTCAG AAGATGCCAT TATGGATTTT 
GTGGGCGAAG CATGTAATAG GTGTGCATTC TACCTGGATA TACTCCAGAA ATGTAGCAGA 
CGTAAGATTA GACAGAATAT TGTTCATATT CTAGAAAATT GGTTGTCAGC TGAACATCTG 
ATTAGACGGT TACCAGGCCC TGAGGCAATT GTAAAGCAAT GGGTTAAGAT AGAGCGGGAA 
TGTCACACGG ATCTGGATGC GGCAGGTTCT TGTACAACTT TGTACTCATT GTTATCATCT 
TCTCAAAAAA AGTCAAAGCG GGGTATCGGA AAAATCCTTG AGCAGGAGCT TCTTGCCTAT 
GACAGAGTGT TACCTTTAAG GTCAAATCTA GGTCAACAAA CGCGAATCAA GATAGCAGAT 
ATCCTCTTGA AGGATGTTTA TGTCACTGAG GATATGCATA TAGAGAGAGC AAGAATTTTG 
ATATGGAAAG CACGAATGAC AAGGACATCT GGAACTGAAC ATATAACCGA GTGCATTTGT 
TTTCTGTCTG AAGCAATCTC TATATTGGGT GAGTTACATC ATGGGCCAAA TGAAGAGGGT 
TCTCCATCTT CTCACATGTT ACCTATTGCG TATTGCTTGA GAGCTTTTTG TACCCAGGAA 
GCTGACCCAA ACTCGAAGAA AGTCTTTCAA GATATTAGCA CTTCACTAAA TCTCTGGCTA 
AGGATTCTTA GCCTAGATGA TAGTGGAGAC AGTCTACCAA CAGAGAATAT AATTCCGTTA 
CTGTATAATA TGATTGATTT GATGTCAGTG AAGGGATGTA CAGAGCTCCA TCATCATATA 
TACCAACTGA TTTTCAGATT GTTTAAATGG AAGAATGTCA AATTGGAGGT CTGCCTTGCA 
ATGCTGTGGG AATGTAGAAG GCTTAGTCAT GCCTTATGCC CTTCCCCCAT AAGTGATGCC 
TTTATCCAGA CCTTATCAGA GAATTGTGCT GACAAATCCA CATGCATTGA TTTCTGGATG 
GATTGCCTGA AAGATTCAAA AGCAAAGTTA ATTGGGTTCC AGCAGAACTT CCATGATTTA 
CATAATAAGG ATGAAGGTCC CTTTCAATCA GACATCACAA TTGACGATAT TAAAGATGCA 
GCATCAGAAC TCATCTCCAG TGCATCACTC TCTGGTAATT CATCTTTTGC AGCTGCATAT 
CTCTATTATG ATCTCTGTGA AAGGCTCATA TCATTTGGAA AACTTTCTGA GGCTCTTTCA 
TATGCAAAAG AAGCCTATAG GATAAGGACT CTTATATTTC AAGATAAATT TAAGTATACA 
GCTGAGAAGC ATATCGAAAA ACACAATGAA GATGGGAAAA TATCAGAAAT ACGGACTTTT 
AGCATAAAAA ATTTCCAAGT CTACAGATTG TTAGCAACTG ATTTTTGGCC ATGTGGAAAT 
TTTCTCTGGG ACATCAATCG CTGCTACCTA AGTCCTTGGA GTGTACTCCA ATGTTATTTG 
GAAAGCACCC TTCAGGTTGG AATTCTGAAT GAGCTCATAG GGAATGGACT GGAGGCAGAA 
ACCATTCTAT CATGGGGGAA AGCCTTTTCA TGCTCGCAAA GTCTATTCCC TTTTGTAGTT 
GCATTTTCTT CTGCCTTAGG AAATCTATAC CACAAAAAGC AGTGTCTGGA TCTGGCAGAA 
AAGGAACTCC AAAATGCAAA AGAGATATTA ATTGCTAATC AGCGAGATTT TTCTTGCGTA 
AAGTGCAAAC TGAAGTTGGA AGTTACATTG GATAAACAAC TTGGAGACAT ATCTCGGAAA 
CAAATTGATA GAGTTTCACA GACAGATGGA TTCTTGCATG CTGAAAGCTT GTTTAGTGCT 
GCCCTGGGAA AATTCTGTTG CTCAGCATGG AAAAGCTGCA TTAGATCACA TGGGGAAGAA 
ATTGCTGAGG AGATAGTGAT TGATAGAAAT GGAGGGGAAG GTTTAGGACA TAATTCAAGT 
AAAACAAAGC TCAGTATAAA GGAGCCACCA GGAAACAGAG GCTCCAGGAG GGGTGGAAGA 
GCTAACAAAA CTTGTTTGTC AAAGGATCAG GATTTGATAT CTGAGCCAAC ATCAAGGTTG 
ACTCGATCTA TGCGTCACTC ACTTAGAGAG CAATGCCAAA ACCGTTCTAA TGTGCCTGAA 
GTTGTTTCAA AGAAGCCTAA TTTATGTGAT AGATCTGTTG GTTCTAGGGG TGAAAGGGTT 
TTGTTGGACA CGAGTAATGC ACTCCCTGGC TTTTGTATTT GCTACAAAGA AAAACGTCAG 
CAGTGCCTCT CGGAAGAAGT AACAGAATCT GGGTCTCTCA ACAATTTAGT AAGTTTGAAA 
TGGGAACTCT GCCATAGGAA GCTTGCGTCC TCAATACTTG TTAGCCTTGG AAAATGTTTG 
GGAGACTCTG GTAGAATTCA TCTAGCTCAT GAGGCTTTAC TGCATAGTAT CTCTGTTTTA 
TTCAAAAGCA CCTGGTCCAG CCACAACCAG CCTTCTGTCA GTCAGTTGCT GGAATTTATC 
GGGAAAGAAG TTACAAGAGA TGTATTTGCC GTTGATCGTG CAATAATATT ATACAACTTG 
TGCTGGTTGA ATTTGCGGAA TTACCACTGC AGGAAGAGTA GGTCTATCTG CTGTGATCTG 
TTTCATATCC CTTTCACAAA ACTAGTGTCA TGGTTAATGT TAGCATTTGT ACTCAGCGGA 
GAGGTTCCAA TATTATTTCA AAAGGTTTCA AGATTGCTTG CCTCTTTATA TCTGCTTTCT 
TCCTCAAATT CTGAATTCAC ATTCGAATCT GATGGAAATG AGCTGTCAGC AAGTCATTGG 
GTCTCATTTT TCCATCAAGC CTCGCTTGGT ACCCATCTCA GTTACCATTT CATTTCAAAT 
TTGAGCCAAA AACACAAATC ACAGTGCCTC TCAGATAAAG AGTGCACAGA GGCTACTTGC 
TCGAGTTGCA TGGTTCCTGA GGATTTGGAC TTACCCAGGC TTGCTCCTGA CCGTACTCAA 
GATCTTGTAC AATTCGCTAA AGAATTTTTC ATCAATCTTC CAAGCTCAAC AATCATCTGC 
ATTAGTTTGC TTGGAGGTGC TTTAAACCAA TTGTTACAGG AGCTAATGCA TATTCGTTCA 
CCTGTTTGTG CCTGGGTGCT CATATCACGC CTAAACCCAG AAAGTCAACC GGTTGCCACA 
CTTTTACCTG TAGATTCGAT TGTAGAAGAT GACAGTGCAA ATCTTAGCTC TACCGAAGCG 
ACTCAAGTCA AGAGTTTAAA GGGGCCCTGG CTTTGTCCAT GGGGTACCAC CGTGGTTGAT 
GAGGTAGCTC CAGCATTTAA ATCAATATTG GAGGAAAGCC ACTCATCTTC TTCTACTACT 
GAAGAAGACA CAATAGAGAG TAGGGGCCTG TGGTGGAAAA AGAGAAAGAA ACTTAATCAT 
CGTCTGGGAA TATTCCTAAG GAATCTAGAA GCTTCCTGGC TGGGTCCCTG GAGGTGTCTG 
CTTCTCGGAG AATGGTCGAA CTACAAATTG CCTGACTCAG CGCAGAAAAA GCTGGTAAAT 
GATCTCAAGT CCAAGTGCAA AATGGAAGTA AATGAGATGC TTTTGAAGGT TATTCTTGGA 
GGTGGGACGG ATAACTTTAA AGGAGAAGCA TGCGTTGCTC AGCTTTCTTT AAGAAATGGT 
TGCTATGTAG GTAGAGGGGG ATATCTTTAT GAAGAAGATA GCTGTAAAAC TCCTACTGCT 
GCGTCTAATA TATCTGAAAG TAGGCATGAA TTGGCCTTAA AACTGATACA TGACGCAGCA 
AGCAAACTAG GGCAACAAGA TGGACATGAA AATAGAGAAC CTATCATTCT TGTGTTAGAT 
CCCGAGGTTC AGATGCTTCC TTGGGAGAAT ATTCCAATAC TAAGGAAACA GGAGGTGTAC 
CGCATGCCTT CCGTAGGTTG CATATCTGCC GTGCTAAAGA AGCGCAGTCT TCAAGGAGAG 
CCAGCAAAAT CTCATGTTGC TTCCTTCCCT CTTATTGATC CGCTAGATTC ATTTTACTTG 
TTGAATCCTG GTGGTGATCT CACCGATACA CAAGTCACGT TTGAGAGTTG GTTCAGAGAT 
CAAAACTTTG AGGGTAAAGC TGGGTCAGAA CCAAGTGCTA TAGAGTTGAC AGAAGCATTA 
GAAACTCATG ATCTCTTTCT ATACTTTGGT CATGGAAGCG GAGCACAGTA TATACCTAGG 
CGTGAAATAG AGAAGCTAGA TAACTGTTCT GCAACTTTCT TAATGGGATG CAGTAGTGGT 
TCACTATGGC TAAAAGGCTG CTACATCCCA CAAGGTGTAC CACTTTCTTA TCTCCTAGGT 
GGATCTCCAG CCATTGTGGC GACTCTATGG GACGTAACAG ACCGAGACAT TGATCGGTTT 
GGGAAAGCGT TGTTGGAAGC ATGGTTGCAA GAGAGGTCAG ATTCTTCTTC AGAGGGTGGC 
TGCAGCCAAT GTGAGTCATT AGCTAATGAC CTTGCAGCAA TGACTCTAAA AGGCACCAAG 
AGGTCAAGGA AACCATCATC GCGTAACAAG CCAGCTCAAT CAGATGTGGA TGGGTCAGGG 
AAGATTGAGT GCAACCACAA GCATAGACGT AAAATCGGTT CATTCATAGC CGCGGCTCGA 
GATGCGTGCA ACTTGCAATA CTTGATTGGG GCTGCGCCTG TCTGTTATGG TGTGCCAACT 
GGTATCACTA GGAAAAAAGG AATTGATGCT CTTCTTCCTT CTTCATCTCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESP ( NM_001341570.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328112.1 Arabidopsis thaliana separase (ESP), partial mRNA.

Price & Availability↑

CloneID	OAb39612
Clone ID Related Accession (Same CDS sequence)	NM_001341570.1
Accession Version	NM_001341570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	separase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). COMPLETENESS: incomplete on the 5' end.

ATGGCGTCCT CTGGCGACGA CCTCCGTCTT CTCTCTCTCA TCGACGTCGG CGATAATGTC 
TTCTCTTCCT TCTCCGATTA CCTTAAACCT TTCTCTACTC TATCCACTTC CCGGAAAAAA 
CAAGACCGAG CAACCACGAT TCGAGCCCTA GCTAAGCAAT TCCTTCCTTT CCTCAACAAA 
TCGATCTCTC TCCTACCGAA ACGTCTCTCC GTCGCGAATT CTGATAAGGA AGCTCGTGAA 
TCAGCATTAG ATCTGTTCCG TGCTTATGAA CTTTGTTTGG ACTGTTTAGA ATTGGTCTCT 
GCTCAATTAG CTTGTAAGCC TCACACTGTT CAATCTCAGA GGCTAAGGAT GATTCATTGT 
CTCGATGTTT GGGGTTTGTA TGAGAATGTG TATACTGAAG CGTTTAAGGT TCTGGAGAAG 
CTAAGAGGCT CTGATAGCAA ATCCCGGAAG TCCCGGTTGT TGCCGGAGGT TCAGGATGGA 
GATGCGGAGA TGGCTTTAGT TGTTGTTGAT GCTGTGGCGG CCATCTTTAG GGCTGTGGCA 
ATGAGTCAGC AGTTAGATGA TAAGCGGTAT CGGAAAGTTC TTCTTTTGCT TGAGGAAGTC 
GGAGGCTGGT TAAGGGTCTT AGATGCTAAG GTGTATGAAA AGTTGCACAG AGCGATGGTG 
ACAAGTATGG GTAAATGTGC TGTTTCTCTC GTTAGAGAAG CTGAGCGTTT CAATGGGGAT 
TTAGTGATTT CTTTCTGCGA CTTAACTGTG AAGGAACATT ATAAATCTGC ATTATCAAAA 
GATCGAGTTT ATAAGTTTGC TCGCGAGGTG CTTTCTGTTC TGTTTGGATT TAAGGATAGA 
AAAATGTCAG TGACCATTGA CATTTCAATG TCTGTGTTGC GCAGCTTATC TTGCCAATTT 
GAGGATGAAA GTAATGAGAA TTTAATGGAG TTTTTTGACC TAGTCGATTA TTGCGCTCAC 
AAGTTTCGAG CAGCAGGAGA CATGTACTGT GCAAAAGTTT CAAAAAAATT GAATGAGATG 
GCAGCTATTT TCGTAGAGGC AATACCCCAA CTTAATTTGG TTCTTAGACT GTATTCGACC 
GGGCTGTCCA TCACGGTTTG CAATTCCAAG CTCGGAGAAA TTAAGCTAGA AGATTCAACA 
GATGATTGGA AGATTCAAGC TATGTTTGAT GATGATGCTA GATGGCAAAG CTTAGTTTCT 
TTACTCGGCA TGGTTGATAG TTACTCAGGT GATGAGGGCA ATCAGACTGG TTCATCATCG 
ATTGGTGGGC ATAGGAACTA CAACAATAAA ACACATGATA GCTGTAAAGA CAGGAACAAG 
ATTACTTGTT GGCCACAATA CGTAGATGCC TTGAAATTCT TGTGCCAACC GCTTGCAGAC 
TTTATATATT CTGTGAAAAG AAAGATTGTG CTGGAGACAG AAATGTCATG CGCCTCTGCT 
CATCTAATTA CTATTCACGA TGCCTTTCTC CAGTTTTGTG ATGGCTGTCT CTTCCTTCAG 
AGGTGCACGT CTGACAAGGG AGATAGAGAA ATTGCCAATA ACAAAGCTTT CTTAAACGCG 
GCTATGGGTG CTTTCATTGT CTCATTGAGA ACCCAACTTA AATTGGAGAT AAGTGCCCAT 
CTAGTTGAGG ATGTAATTGG AAGCCCATGG ATCCAATCTC AAGAACTCAA GTATCTAATT 
GCGACGCTGT ACAATATTGG TATCGTTTTG TACAGAAATA AGGAGCTAAA CAAGGCTTGT 
GAGGCGCTTA AGCTGTGCTC CAAGGTATCA TGGAGATGTG TTGAACTACA TTGTCATATG 
TTTGTAAATC AGTCTAGTTC ATCTGATAAT GACTTGTCAG AAGATGCCAT TATGGATTTT 
GTGGGCGAAG CATGTAATAG GTGTGCATTC TACCTGGATA TACTCCAGAA ATGTAGCAGA 
CGTAAGATTA GACAGAATAT TGTTCATATT CTAGAAAATT GGTTGTCAGC TGAACATCTG 
ATTAGACGGT TACCAGGCCC TGAGGCAATT GTAAAGCAAT GGGTTAAGAT AGAGCGGGAA 
TGTCACACGG ATCTGGATGC GGCAGGTTCT TGTACAACTT TGTACTCATT GTTATCATCT 
TCTCAAAAAA AGTCAAAGCG GGGTATCGGA AAAATCCTTG AGCAGGAGCT TCTTGCCTAT 
GACAGAGTGT TACCTTTAAG GTCAAATCTA GGTCAACAAA CGCGAATCAA GATAGCAGAT 
ATCCTCTTGA AGGATGTTTA TGTCACTGAG GATATGCATA TAGAGAGAGC AAGAATTTTG 
ATATGGAAAG CACGAATGAC AAGGACATCT GGAACTGAAC ATATAACCGA GTGCATTTGT 
TTTCTGTCTG AAGCAATCTC TATATTGCAG GGTGAGTTAC ATCATGGGCC AAATGAAGAG 
GGTTCTCCAT CTTCTCACAT GTTACCTATT GCGTATTGCT TGAGAGCTTT TTGTACCCAG 
GAAGCTGACC CAAACTCGAA GAAAGTCTTT CAAGATATTA GCACTTCACT AAATCTCTGG 
CTAAGGATTC TTAGCCTAGA TGATAGTGGA GACAGTCTAC CAACAGAGAA TATAATTCCG 
TTACTGTATA ATATGATTGA TTTGATGTCA GTGAAGGGAT GTACAGAGCT CCATCATCAT 
ATATACCAAC TGATTTTCAG ATTGTTTAAA TGGAAGAATG TCAAATTGGA GGTCTGCCTT 
GCAATGCTGT GGGAATGTAG AAGGCTTAGT CATGCCTTAT GCCCTTCCCC CATAAGTGAT 
GCCTTTATCC AGACCTTATC AGAGAATTGT GCTGACAAAT CCACATGCAT TGATTTCTGG 
ATGGATTGCC TGAAAGATTC AAAAGCAAAG TTAATTGGGT TCCAGCAGAA CTTCCATGAT 
TTACATAATA AGGATGAAGG TCCCTTTCAA TCAGACATCA CAATTGACGA TATTAAAGAT 
GCAGCATCAG AACTCATCTC CAGTGCATCA CTCTCTGGTA ATTCATCTTT TGCAGCTGCA 
TATCTCTATT ATGATCTCTG TGAAAGGCTC ATATCATTTG GAAAACTTTC TGAGGCTCTT 
TCATATGCAA AAGAAGCCTA TAGGATAAGG ACTCTTATAT TTCAAGATAA ATTTAAGTAT 
ACAGCTGAGA AGCATATCGA AAAACACAAT GAAGATGGGA AAATATCAGA AATACGGACT 
TTTAGCATAA AAAATTTCCA AGTCTACAGA TTGTTAGCAA CTGATTTTTG GCCATGTGGA 
AATTTTCTCT GGGACATCAA TCGCTGCTAC CTAAGTCCTT GGAGTGTACT CCAATGTTAT 
TTGGAAAGCA CCCTTCAGGT TGGAATTCTG AATGAGCTCA TAGGGAATGG ACTGGAGGCA 
GAAACCATTC TATCATGGGG GAAAGCCTTT TCATGCTCGC AAAGTCTATT CCCTTTTGTA 
GTTGCATTTT CTTCTGCCTT AGGAAATCTA TACCACAAAA AGCAGTGTCT GGATCTGGCA 
GAAAAGGAAC TCCAAAATGC AAAAGAGATA TTAATTGCTA ATCAGCGAGA TTTTTCTTGC 
GTAAAGTGCA AACTGAAGTT GGAAGTTACA TTGGATAAAC AACTTGGAGA CATATCTCGG 
AAACAAATTG ATAGAGTTTC ACAGACAGAT GGATTCTTGC ATGCTGAAAG CTTGTTTAGT 
GCTGCCCTGG GAAAATTCTG TTGCTCAGCA TGGAAAAGCT GCATTAGATC ACATGGGGAA 
GAAATTGCTG AGGAGATAGT GATTGATAGA AATGGAGGGG AAGGTTTAGG ACATAATTCA 
AGTAAAACAA AGCTCAGTAT AAAGGAGCCA CCAGGAAACA GAGGCTCCAG GAGGGGTGGA 
AGAGCTAACA AAACTTGTTT GTCAAAGGAT CAGGATTTGA TATCTGAGCC AACATCAAGG 
TTGACTCGAT CTATGCGTCA CTCACTTAGA GAGCAATGCC AAAACCGTTC TAATGTGCCT 
GAAGTTGTTT CAAAGAAGCC TAATTTATGT GATAGATCTG TTGGTTCTAG GGGTGAAAGG 
GTTTTGTTGG ACACGAGTAA TGCACTCCCT GGCTTTTGTA TTTGCTACAA AGAAAAACGT 
CAGCAGTGCC TCTCGGAAGA AGTAACAGAA TCTGGGTCTC TCAACAATTT AGTAAGTTTG 
AAATGGGAAC TCTGCCATAG GAAGCTTGCG TCCTCAATAC TTGTTAGCCT TGGAAAATGT 
TTGGGAGACT CTGGTAGAAT TCATCTAGCT CATGAGGCTT TACTGCATAG TATCTCTGTT 
TTATTCAAAA GCACCTGGTC CAGCCACAAC CAGCCTTCTG TCAGTCAGTT GCTGGAATTT 
ATCGGGAAAG AAGTTACAAG AGATGTATTT GCCGTTGATC GTGCAATAAT ATTATACAAC 
TTGTGCTGGT TGAATTTGCG GAATTACCAC TGCAGGAAGA GTAGGTCTAT CTGCTGTGAT 
CTGTTTCATA TCCCTTTCAC AAAACTAGTG TCATGGTTAA TGTTAGCATT TGTACTCAGC 
GGAGAGGTTC CAATATTATT TCAAAAGGTT TCAAGATTGC TTGCCTCTTT ATATCTGCTT 
TCTTCCTCAA ATTCTGAATT CACATTCGAA TCTGATGGAA ATGAGCTGTC AGCAAGTCAT 
TGGGTCTCAT TTTTCCATCA AGCCTCGCTT GGTACCCATC TCAGTTACCA TTTCATTTCA 
AATTTGAGCC AAAAACACAA ATCACAGTGC CTCTCAGATA AAGAGTGCAC AGAGGCTACT 
TGCTCGAGTT GCATGGTTCC TGAGGATTTG GACTTACCCA GGCTTGCTCC TGACCGTACT 
CAAGATCTTG TACAATTCGC TAAAGAATTT TTCATCAATC TTCCAAGCTC AACAATCATC 
TGCATTAGTT TGCTTGGAGG TGCTTTAAAC CAATTGTTAC AGGAGCTAAT GCATATTCGT 
TCACCTGTTT GTGCCTGGGT GCTCATATCA CGCCTAAACC CAGAAAGTCA ACCGGTTGCC 
ACACTTTTAC CTGTAGATTC GATTGTAGAA GACATGTCAG ATGACAGTGC AAATCTTAGC 
TCTACCGAAG CGACTCAAGT CAAGAGTTTA AAGGGGCCCT GGCTTTGTCC ATGGGGTACC 
ACCGTGGTTG ATGAGGTAGC TCCAGCATTT AAATCAATAT TGGAGGAAAG CCACTCATCT 
TCTTCTACTA CTGAAGAAGA CACAATAGAG AGTAGGGGCC TGTGGTGGAA AAAGAGAAAG 
AAACTTAATC ATCGTCTGGG AATATTCCTA AGGAATCTAG AAGCTTCCTG GCTGGGTCCC 
TGGAGGTGTC TGCTTCTCGG AGAATGGTCG AACTACAAAT TGCCTGACTC AGCGCAGAAA 
AAGCTGGTAA ATGATCTCAA GTCCAAGTGC AAAATGGAAG TAAATGAGAT GCTTTTGAAG 
GTTATTCTTG GAGGTGGGAC GGATAACTTT AAAGGAGAAG CATGCGTTGC TCAGCTTTCT 
TTAAGAAATG GTTGCTATGT AGGTAGAGGG GGATATCTTT ATGAAGAAGA TAGCTGTAAA 
ACTCCTACTG CTGCGTCTAA TATATCTGAA AGTAGGCATG AATTGGCCTT AAAACTGATA 
CATGACGCAG CAAGCAAACT AGGGCAACAA GATGGACATG AAAATAGAGA ACCTATCATT 
CTTGTGTTAG ATCCCGAGGT TCAGATGCTT CCTTGGGAGA ATATTCCAAT ACTAAGGAAA 
CAGGAGGTGT ACCGCATGCC TTCCGTAGGT TGCATATCTG CCGTGCTAAA GAAGCGCAGT 
CTTCAAGGAG AGCCAGCAAA ATCTCATGTT GCTTCCTTCC CTCTTATTGA TCCGCTAGAT 
TCATTTTACT TGTTGAATCC TGGTGGTGAT CTCACCGATA CACAAGTCAC GTTTGAGAGT 
TGGTTCAGAG ATCAAAACTT TGAGGGTAAA GCTGGGTCAG AACCAAGTGC TATAGAGTTG 
ACAGAAGCAT TAGAAACTCA TGATCTCTTT CTATACTTTG GTCATGGAAG CGGAGCACAG 
TATATACCTA GGCGTGAAAT AGAGAAGCTA GATAACTGTT CTGCAACTTT CTTAATGGGA 
TGCAGTAGTG GTTCACTATG GCTAAAAGGC TGCTACATCC CACAAGGTGT ACCACTTTCT 
TATCTCCTAG GTGGATCTCC AGCCATTGTG GCGACTCTAT GGGACGTAAC AGACCGAGAC 
ATTGATCGGT TTGGGAAAGC GTTGTTGGAA GCATGGTTGC AAGAGAGGTC AGATTCTTCT 
TCAGAGGGTG GCTGCAGCCA ATGTGAGTCA TTAGCTAATG ACCTTGCAGC AATGACTCTA 
AAAGGCACCA AGAGGTCAAG GAAACCATCA TCGCGTAACA AGCCAGCTCA ATCAGATGTG 
GATGGGTCAG GGAAGATTGA GTGCAACCAC AAGCATAGAC GTAAAATCGG TTCATTCATA 
GCCGCGGCTC GAGATGCGTG CAACTTGCAA TACTTGATTG GGGCTGCGCC TGTCTGTTAT 
GGTGTGCCAA CTGGTATCAC TAGGAAAAAA GGAATTGATG CTCTTCTTCC TTCTTCATCT 
CGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328112.1
CDS	1..6546
Translation	MASSGDDLRLLSLIDVGDNVFSSFSDYLKPFSTLSTSRKKQDRATTIRALAKQFLPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQRLRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPEVQDGDAEMALVVVDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAVSLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMSVTIDISMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVEAIPQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVDSYSGDEGNQTGSSSIGGHRNYNNKTHDSCKDRNKITCWPQYVDALKFLCQPLADFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLFLQRCTSDKGDREIANNKAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYRNKELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYLDILQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCTTLYSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDMHIERARILIWKARMTRTSGTEHITECICFLSEAISILQGELHHGPNEEGSPSSHMLPIAYCLRAFCTQEADPNSKKVFQDISTSLNLWLRILSLDDSGDSLPTENIIPLLYNMIDLMSVKGCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSENCADKSTCIDFWMDCLKDSKAKLIGFQQNFHDLHNKDEGPFQSDITIDDIKDAASELISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTAEKHIEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTLQVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQNAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRVSQTDGFLHAESLFSAALGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRANKTCLSKDQDLISEPTSRLTRSMRHSLREQCQNRSNVPEVVSKKPNLCDRSVGSRGERVLLDTSNALPGFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASSILVSLGKCLGDSGRIHLAHEALLHSISVLFKSTWSSHNQPSVSQLLEFIGKEVTRDVFAVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKVSRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQCLSDKECTEATCSSCMVPEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIICISLLGGALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDMSDDSANLSSTEATQVKSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSSTTEEDTIESRGLWWKKRKKLNHRLGIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLKVILGGGTDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLIHDAASKLGQQDGHENREPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKKRSLQGEPAKSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAIELTEALETHDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVPLSYLLGGSPAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAMTLKGTKRSRKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPVCYGVPTGITRKKGIDALLPSSSR

Target ORF information:

RefSeq Version	NM_001341570.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana separase (ESP), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341570.1

ORF Insert Sequence:

ATGGCGTCCT CTGGCGACGA CCTCCGTCTT CTCTCTCTCA TCGACGTCGG CGATAATGTC 
TTCTCTTCCT TCTCCGATTA CCTTAAACCT TTCTCTACTC TATCCACTTC CCGGAAAAAA 
CAAGACCGAG CAACCACGAT TCGAGCCCTA GCTAAGCAAT TCCTTCCTTT CCTCAACAAA 
TCGATCTCTC TCCTACCGAA ACGTCTCTCC GTCGCGAATT CTGATAAGGA AGCTCGTGAA 
TCAGCATTAG ATCTGTTCCG TGCTTATGAA CTTTGTTTGG ACTGTTTAGA ATTGGTCTCT 
GCTCAATTAG CTTGTAAGCC TCACACTGTT CAATCTCAGA GGCTAAGGAT GATTCATTGT 
CTCGATGTTT GGGGTTTGTA TGAGAATGTG TATACTGAAG CGTTTAAGGT TCTGGAGAAG 
CTAAGAGGCT CTGATAGCAA ATCCCGGAAG TCCCGGTTGT TGCCGGAGGT TCAGGATGGA 
GATGCGGAGA TGGCTTTAGT TGTTGTTGAT GCTGTGGCGG CCATCTTTAG GGCTGTGGCA 
ATGAGTCAGC AGTTAGATGA TAAGCGGTAT CGGAAAGTTC TTCTTTTGCT TGAGGAAGTC 
GGAGGCTGGT TAAGGGTCTT AGATGCTAAG GTGTATGAAA AGTTGCACAG AGCGATGGTG 
ACAAGTATGG GTAAATGTGC TGTTTCTCTC GTTAGAGAAG CTGAGCGTTT CAATGGGGAT 
TTAGTGATTT CTTTCTGCGA CTTAACTGTG AAGGAACATT ATAAATCTGC ATTATCAAAA 
GATCGAGTTT ATAAGTTTGC TCGCGAGGTG CTTTCTGTTC TGTTTGGATT TAAGGATAGA 
AAAATGTCAG TGACCATTGA CATTTCAATG TCTGTGTTGC GCAGCTTATC TTGCCAATTT 
GAGGATGAAA GTAATGAGAA TTTAATGGAG TTTTTTGACC TAGTCGATTA TTGCGCTCAC 
AAGTTTCGAG CAGCAGGAGA CATGTACTGT GCAAAAGTTT CAAAAAAATT GAATGAGATG 
GCAGCTATTT TCGTAGAGGC AATACCCCAA CTTAATTTGG TTCTTAGACT GTATTCGACC 
GGGCTGTCCA TCACGGTTTG CAATTCCAAG CTCGGAGAAA TTAAGCTAGA AGATTCAACA 
GATGATTGGA AGATTCAAGC TATGTTTGAT GATGATGCTA GATGGCAAAG CTTAGTTTCT 
TTACTCGGCA TGGTTGATAG TTACTCAGGT GATGAGGGCA ATCAGACTGG TTCATCATCG 
ATTGGTGGGC ATAGGAACTA CAACAATAAA ACACATGATA GCTGTAAAGA CAGGAACAAG 
ATTACTTGTT GGCCACAATA CGTAGATGCC TTGAAATTCT TGTGCCAACC GCTTGCAGAC 
TTTATATATT CTGTGAAAAG AAAGATTGTG CTGGAGACAG AAATGTCATG CGCCTCTGCT 
CATCTAATTA CTATTCACGA TGCCTTTCTC CAGTTTTGTG ATGGCTGTCT CTTCCTTCAG 
AGGTGCACGT CTGACAAGGG AGATAGAGAA ATTGCCAATA ACAAAGCTTT CTTAAACGCG 
GCTATGGGTG CTTTCATTGT CTCATTGAGA ACCCAACTTA AATTGGAGAT AAGTGCCCAT 
CTAGTTGAGG ATGTAATTGG AAGCCCATGG ATCCAATCTC AAGAACTCAA GTATCTAATT 
GCGACGCTGT ACAATATTGG TATCGTTTTG TACAGAAATA AGGAGCTAAA CAAGGCTTGT 
GAGGCGCTTA AGCTGTGCTC CAAGGTATCA TGGAGATGTG TTGAACTACA TTGTCATATG 
TTTGTAAATC AGTCTAGTTC ATCTGATAAT GACTTGTCAG AAGATGCCAT TATGGATTTT 
GTGGGCGAAG CATGTAATAG GTGTGCATTC TACCTGGATA TACTCCAGAA ATGTAGCAGA 
CGTAAGATTA GACAGAATAT TGTTCATATT CTAGAAAATT GGTTGTCAGC TGAACATCTG 
ATTAGACGGT TACCAGGCCC TGAGGCAATT GTAAAGCAAT GGGTTAAGAT AGAGCGGGAA 
TGTCACACGG ATCTGGATGC GGCAGGTTCT TGTACAACTT TGTACTCATT GTTATCATCT 
TCTCAAAAAA AGTCAAAGCG GGGTATCGGA AAAATCCTTG AGCAGGAGCT TCTTGCCTAT 
GACAGAGTGT TACCTTTAAG GTCAAATCTA GGTCAACAAA CGCGAATCAA GATAGCAGAT 
ATCCTCTTGA AGGATGTTTA TGTCACTGAG GATATGCATA TAGAGAGAGC AAGAATTTTG 
ATATGGAAAG CACGAATGAC AAGGACATCT GGAACTGAAC ATATAACCGA GTGCATTTGT 
TTTCTGTCTG AAGCAATCTC TATATTGCAG GGTGAGTTAC ATCATGGGCC AAATGAAGAG 
GGTTCTCCAT CTTCTCACAT GTTACCTATT GCGTATTGCT TGAGAGCTTT TTGTACCCAG 
GAAGCTGACC CAAACTCGAA GAAAGTCTTT CAAGATATTA GCACTTCACT AAATCTCTGG 
CTAAGGATTC TTAGCCTAGA TGATAGTGGA GACAGTCTAC CAACAGAGAA TATAATTCCG 
TTACTGTATA ATATGATTGA TTTGATGTCA GTGAAGGGAT GTACAGAGCT CCATCATCAT 
ATATACCAAC TGATTTTCAG ATTGTTTAAA TGGAAGAATG TCAAATTGGA GGTCTGCCTT 
GCAATGCTGT GGGAATGTAG AAGGCTTAGT CATGCCTTAT GCCCTTCCCC CATAAGTGAT 
GCCTTTATCC AGACCTTATC AGAGAATTGT GCTGACAAAT CCACATGCAT TGATTTCTGG 
ATGGATTGCC TGAAAGATTC AAAAGCAAAG TTAATTGGGT TCCAGCAGAA CTTCCATGAT 
TTACATAATA AGGATGAAGG TCCCTTTCAA TCAGACATCA CAATTGACGA TATTAAAGAT 
GCAGCATCAG AACTCATCTC CAGTGCATCA CTCTCTGGTA ATTCATCTTT TGCAGCTGCA 
TATCTCTATT ATGATCTCTG TGAAAGGCTC ATATCATTTG GAAAACTTTC TGAGGCTCTT 
TCATATGCAA AAGAAGCCTA TAGGATAAGG ACTCTTATAT TTCAAGATAA ATTTAAGTAT 
ACAGCTGAGA AGCATATCGA AAAACACAAT GAAGATGGGA AAATATCAGA AATACGGACT 
TTTAGCATAA AAAATTTCCA AGTCTACAGA TTGTTAGCAA CTGATTTTTG GCCATGTGGA 
AATTTTCTCT GGGACATCAA TCGCTGCTAC CTAAGTCCTT GGAGTGTACT CCAATGTTAT 
TTGGAAAGCA CCCTTCAGGT TGGAATTCTG AATGAGCTCA TAGGGAATGG ACTGGAGGCA 
GAAACCATTC TATCATGGGG GAAAGCCTTT TCATGCTCGC AAAGTCTATT CCCTTTTGTA 
GTTGCATTTT CTTCTGCCTT AGGAAATCTA TACCACAAAA AGCAGTGTCT GGATCTGGCA 
GAAAAGGAAC TCCAAAATGC AAAAGAGATA TTAATTGCTA ATCAGCGAGA TTTTTCTTGC 
GTAAAGTGCA AACTGAAGTT GGAAGTTACA TTGGATAAAC AACTTGGAGA CATATCTCGG 
AAACAAATTG ATAGAGTTTC ACAGACAGAT GGATTCTTGC ATGCTGAAAG CTTGTTTAGT 
GCTGCCCTGG GAAAATTCTG TTGCTCAGCA TGGAAAAGCT GCATTAGATC ACATGGGGAA 
GAAATTGCTG AGGAGATAGT GATTGATAGA AATGGAGGGG AAGGTTTAGG ACATAATTCA 
AGTAAAACAA AGCTCAGTAT AAAGGAGCCA CCAGGAAACA GAGGCTCCAG GAGGGGTGGA 
AGAGCTAACA AAACTTGTTT GTCAAAGGAT CAGGATTTGA TATCTGAGCC AACATCAAGG 
TTGACTCGAT CTATGCGTCA CTCACTTAGA GAGCAATGCC AAAACCGTTC TAATGTGCCT 
GAAGTTGTTT CAAAGAAGCC TAATTTATGT GATAGATCTG TTGGTTCTAG GGGTGAAAGG 
GTTTTGTTGG ACACGAGTAA TGCACTCCCT GGCTTTTGTA TTTGCTACAA AGAAAAACGT 
CAGCAGTGCC TCTCGGAAGA AGTAACAGAA TCTGGGTCTC TCAACAATTT AGTAAGTTTG 
AAATGGGAAC TCTGCCATAG GAAGCTTGCG TCCTCAATAC TTGTTAGCCT TGGAAAATGT 
TTGGGAGACT CTGGTAGAAT TCATCTAGCT CATGAGGCTT TACTGCATAG TATCTCTGTT 
TTATTCAAAA GCACCTGGTC CAGCCACAAC CAGCCTTCTG TCAGTCAGTT GCTGGAATTT 
ATCGGGAAAG AAGTTACAAG AGATGTATTT GCCGTTGATC GTGCAATAAT ATTATACAAC 
TTGTGCTGGT TGAATTTGCG GAATTACCAC TGCAGGAAGA GTAGGTCTAT CTGCTGTGAT 
CTGTTTCATA TCCCTTTCAC AAAACTAGTG TCATGGTTAA TGTTAGCATT TGTACTCAGC 
GGAGAGGTTC CAATATTATT TCAAAAGGTT TCAAGATTGC TTGCCTCTTT ATATCTGCTT 
TCTTCCTCAA ATTCTGAATT CACATTCGAA TCTGATGGAA ATGAGCTGTC AGCAAGTCAT 
TGGGTCTCAT TTTTCCATCA AGCCTCGCTT GGTACCCATC TCAGTTACCA TTTCATTTCA 
AATTTGAGCC AAAAACACAA ATCACAGTGC CTCTCAGATA AAGAGTGCAC AGAGGCTACT 
TGCTCGAGTT GCATGGTTCC TGAGGATTTG GACTTACCCA GGCTTGCTCC TGACCGTACT 
CAAGATCTTG TACAATTCGC TAAAGAATTT TTCATCAATC TTCCAAGCTC AACAATCATC 
TGCATTAGTT TGCTTGGAGG TGCTTTAAAC CAATTGTTAC AGGAGCTAAT GCATATTCGT 
TCACCTGTTT GTGCCTGGGT GCTCATATCA CGCCTAAACC CAGAAAGTCA ACCGGTTGCC 
ACACTTTTAC CTGTAGATTC GATTGTAGAA GACATGTCAG ATGACAGTGC AAATCTTAGC 
TCTACCGAAG CGACTCAAGT CAAGAGTTTA AAGGGGCCCT GGCTTTGTCC ATGGGGTACC 
ACCGTGGTTG ATGAGGTAGC TCCAGCATTT AAATCAATAT TGGAGGAAAG CCACTCATCT 
TCTTCTACTA CTGAAGAAGA CACAATAGAG AGTAGGGGCC TGTGGTGGAA AAAGAGAAAG 
AAACTTAATC ATCGTCTGGG AATATTCCTA AGGAATCTAG AAGCTTCCTG GCTGGGTCCC 
TGGAGGTGTC TGCTTCTCGG AGAATGGTCG AACTACAAAT TGCCTGACTC AGCGCAGAAA 
AAGCTGGTAA ATGATCTCAA GTCCAAGTGC AAAATGGAAG TAAATGAGAT GCTTTTGAAG 
GTTATTCTTG GAGGTGGGAC GGATAACTTT AAAGGAGAAG CATGCGTTGC TCAGCTTTCT 
TTAAGAAATG GTTGCTATGT AGGTAGAGGG GGATATCTTT ATGAAGAAGA TAGCTGTAAA 
ACTCCTACTG CTGCGTCTAA TATATCTGAA AGTAGGCATG AATTGGCCTT AAAACTGATA 
CATGACGCAG CAAGCAAACT AGGGCAACAA GATGGACATG AAAATAGAGA ACCTATCATT 
CTTGTGTTAG ATCCCGAGGT TCAGATGCTT CCTTGGGAGA ATATTCCAAT ACTAAGGAAA 
CAGGAGGTGT ACCGCATGCC TTCCGTAGGT TGCATATCTG CCGTGCTAAA GAAGCGCAGT 
CTTCAAGGAG AGCCAGCAAA ATCTCATGTT GCTTCCTTCC CTCTTATTGA TCCGCTAGAT 
TCATTTTACT TGTTGAATCC TGGTGGTGAT CTCACCGATA CACAAGTCAC GTTTGAGAGT 
TGGTTCAGAG ATCAAAACTT TGAGGGTAAA GCTGGGTCAG AACCAAGTGC TATAGAGTTG 
ACAGAAGCAT TAGAAACTCA TGATCTCTTT CTATACTTTG GTCATGGAAG CGGAGCACAG 
TATATACCTA GGCGTGAAAT AGAGAAGCTA GATAACTGTT CTGCAACTTT CTTAATGGGA 
TGCAGTAGTG GTTCACTATG GCTAAAAGGC TGCTACATCC CACAAGGTGT ACCACTTTCT 
TATCTCCTAG GTGGATCTCC AGCCATTGTG GCGACTCTAT GGGACGTAAC AGACCGAGAC 
ATTGATCGGT TTGGGAAAGC GTTGTTGGAA GCATGGTTGC AAGAGAGGTC AGATTCTTCT 
TCAGAGGGTG GCTGCAGCCA ATGTGAGTCA TTAGCTAATG ACCTTGCAGC AATGACTCTA 
AAAGGCACCA AGAGGTCAAG GAAACCATCA TCGCGTAACA AGCCAGCTCA ATCAGATGTG 
GATGGGTCAG GGAAGATTGA GTGCAACCAC AAGCATAGAC GTAAAATCGG TTCATTCATA 
GCCGCGGCTC GAGATGCGTG CAACTTGCAA TACTTGATTG GGGCTGCGCC TGTCTGTTAT 
GGTGTGCCAA CTGGTATCAC TAGGAAAAAA GGAATTGATG CTCTTCTTCC TTCTTCATCT 
CGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

ESP cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報