TPS5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TPS5
Entrez Gene ID	827498
Full Name	trehalose phosphatase/synthase 5
Synonyms	ATTPS5,DL4920W,FCAALL.9,TREHALOSE -6-PHOSPHATASE SYNTHASE S5,trehalose phosphatase/synthase 5
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_117886.3	NP_567538.1	trehalose phosphatase/synthase 5
NM_001341241.1	NP_001329809.1	trehalose phosphatase/synthase 5

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	TPS5	NP_567538.1
Schizosaccharomyces pombe (fission yeast)	tps2	NP_594975.1
Oryza sativa (rice)	Os09g0397300	NP_001063104.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

2-deoxyglucose stimulates Threonine 49 phosphorylation of endogenous TPS5 in Arabidopsis cells.
Title: Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose.

The following TPS5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPS5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15239	NM_117886.3 Latest version!	Arabidopsis thaliana trehalose phosphatase/synthase 5 (TPS5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb15239	NM_001341241.1 Latest version!	Arabidopsis thaliana trehalose phosphatase/synthase 5 (TPS5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPS5 ( NM_117886.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567538.1 Arabidopsis thaliana trehalose phosphatase/synthase 5 (TPS5), mRNA.

Price & Availability↑

CloneID	OAb15239
Clone ID Related Accession (Same CDS sequence)	NM_117886.3 , NM_001341241.1
Accession Version	NM_117886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	trehalose phosphatase/synthase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_117886.2.

ATGGTATCAA GATCTTATTC AAACCTCTTG GATCTTGCTT CTGGTAACTT CCATTCGTTT 
TCTCGAGAAA AGAAGAGGTT TCCAAGAGTA GCAACTGTCA CTGGTGTCTT ATCTGAGCTA 
GATGATGATA ACAACAGCAA CAGTGTCTGC TCTGATGCTC CTTCTTCCGT CACACAAGAT 
CGAATCATCA TCGTTGGAAA CCAGCTTCCT ATTAAATCAC ATCGGAACTC TGCTGGTAAA 
TTGAGTTTTA GTTGGGACAA TGACTCGCTT CTCTTGCAGC TTAAAGATGG TATGCGTGAA 
GATATGGAAG TTGTCTACAT TGGTTGCCTT AAGGAACAGA TTGATACAGT TGAGCAAGAT 
GATGTTTCTC AAAGGTTGCT TGAGAATTTC AAATGTGTTC CTGCTTATAT CCCACCTGAG 
CTATTCACTA AGTACTATCA TGGATTCTGT AAGCAACATC TATGGCCTTT GTTTCACTAC 
ATGCTTCCCT TAACTCCTGA TCTTGGTGGT AGATTTGATC GTTCTTTATG GCAAGCTTAT 
CTTTCGGTTA ACAAGATCTT TGCTGATAAA GTGATGGAAG TGATTAGTCC TGATGATGAT 
TTTGTTTGGG TTCATGACTA TCACTTAATG GTTTTGCCTA CATTTCTAAG GAAGAGGTTT 
AATAGAGTAA AGCTTGGTTT TTTCCTTCAT AGCCCGTTCC CTTCCTCTGA GATTTACCGT 
ACTCTTCCAG TGAGAAATGA GCTCTTACGT GCACTCCTCA ACGCTGATTT GATTGGCTTT 
CATACCTTTG ACTATGCAAG ACACTTCCTT TCTTGTTGTA GCAGGATGCT TGGTTTATCC 
TATCAGTCCA AACGTGGAAC CATAGGGCTT GAGTATTATG GTCGAACGGT TAGTATCAAG 
ATTCTTCCTG TGGGGATTCA TATCAGCCAG CTTCAGTCAA TTTTAAACCT CCCAGAGACT 
CAGACCAAAG TTGCTGAGCT AAGAGATCAG TTCTTGGATC AGAAAGTTCT TCTCGGTGTT 
GATGACATGG ACATCTTCAA AGGAATCAGC CTCAAACTCT TGGCAATGGA ACAACTTCTC 
ACACAGCATC CCGAGAAGAG AGGTCGAGTT GTACTTGTCC AGATTGCAAA CCCTGCAAGA 
GGTCGTGGGA AAGACGTTCA GGAGGTTCAG TCTGAAACTG AAGCCACGGT TAAAAGGATC 
AATGAAATGT TTGGAAGGCC GGGCTACCAA CCCGTGGTTC TGATTGATAC ACCGCTTCAA 
TTCTTTGAGA GGATTGCTTA CTATGTCATT GCAGAGTGTT GTCTTGTTAC AGCGGTAAGA 
GATGGTATGA ATCTTATACC TTATGAGTAC ATTATCTGCA GGCAAGGTAA TCCGAAACTC 
AACGAGACTA TAGGCCTTGA CCCTTCTGCT GCAAAGAAGA GTATGCTTGT TGTCTCTGAG 
TTTATTGGTT GTTCTCCTTC TTTAAGCGGC GCCATTAGAG TAAATCCGTG GAACATTGAT 
GCTGTGACTG AAGCAATGGA CTATGCATTG ATAGTTTCAG AAGCAGAGAA GCAAATGCGT 
CACGAGAAGC ATCACAAATA TGTTAGCACA CATGATGTTG CTTATTGGGC GCGTAGCTTT 
ATACAAGATC TTGAAAGGGC TTGCGGGGAT CATGTGAGGA AGAGGTGTTG GGGGATTGGA 
TTCGGGTTAG GCTTTAGAGT TGTGGCGCTT GATCCGAGTT TTAAAAAGCT TTCGATTGAG 
CACATTGTCT CAGCTTATAA GAGAACCAAG AACCGAGCCA TTTTGTTGGA TTATGATGGC 
ACAATGGTGC AGCCAGGTTC CATTAGGACA ACACCAACCC GCGAAACAAT CGAAATCTTG 
AACAACCTGT CTAGTGATCC CAAGAATATC GTGTACCTCG TCAGTGGGAA AGACAGAAGG 
ACACTAACTG AATGGTTTTC TTCATGTGAC GATCTCGGTT TGGGTGCAGA GCACGGGTAT 
TTTATAAGGC CAAATGATGG AACAGACTGG GAAACGTCGA GTTTGGTATC AGGTTTTGAG 
TGGAAACAAA TAGCAGAGCC AGTGATGAGA CTTTACACTG AGACTACAGA TGGATCAACA 
ATAGAGACTA AAGAGACTGC TCTCGTTTGG AATTACCAAT TCGCAGATCC TGATTTTGGA 
TCTTGTCAAG CCAAAGAGCT TATGGAACAC CTCGAAAGCG TGCTTACCAA TGATCCAGTC 
TCTGTCAAGA CTGGACAACA ACTCGTTGAA GTTAAACCAC AGGGTGTGAA CAAAGGTCTT 
GTGGCAGAGA GGCTTCTAAC AACGATGCAA GAAAAAGGGA AACTTTTGGA TTTCATTCTC 
TGCGTCGGTG ATGATCGGTC TGATGAGGAT ATGTTTGAGG TGATAATGAG TGCTAAAGAT 
GGTCCAGCTT TGTCTCCTGT GGCTGAGATA TTCGCTTGCA CCGTTGGACA AAAGCCAAGC 
AAAGCAAAAT ACTATTTAGA CGATACTGCG GAGATAATCA GAATGCTGGA CGGTCTAGCC 
GCTACCAACA CAACTATCTC TGATCAAACT GATTCAACCG CCACTGTTCC AACTAAAGAT 
CTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567538.1
CDS	360..2948
Translation	MVSRSYSNLLDLASGNFHSFSREKKRFPRVATVTGVLSELDDDNNSNSVCSDAPSSVTQDRIIIVGNQLPIKSHRNSAGKLSFSWDNDSLLLQLKDGMREDMEVVYIGCLKEQIDTVEQDDVSQRLLENFKCVPAYIPPELFTKYYHGFCKQHLWPLFHYMLPLTPDLGGRFDRSLWQAYLSVNKIFADKVMEVISPDDDFVWVHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPSSEIYRTLPVRNELLRALLNADLIGFHTFDYARHFLSCCSRMLGLSYQSKRGTIGLEYYGRTVSIKILPVGIHISQLQSILNLPETQTKVAELRDQFLDQKVLLGVDDMDIFKGISLKLLAMEQLLTQHPEKRGRVVLVQIANPARGRGKDVQEVQSETEATVKRINEMFGRPGYQPVVLIDTPLQFFERIAYYVIAECCLVTAVRDGMNLIPYEYIICRQGNPKLNETIGLDPSAAKKSMLVVSEFIGCSPSLSGAIRVNPWNIDAVTEAMDYALIVSEAEKQMRHEKHHKYVSTHDVAYWARSFIQDLERACGDHVRKRCWGIGFGLGFRVVALDPSFKKLSIEHIVSAYKRTKNRAILLDYDGTMVQPGSIRTTPTRETIEILNNLSSDPKNIVYLVSGKDRRTLTEWFSSCDDLGLGAEHGYFIRPNDGTDWETSSLVSGFEWKQIAEPVMRLYTETTDGSTIETKETALVWNYQFADPDFGSCQAKELMEHLESVLTNDPVSVKTGQQLVEVKPQGVNKGLVAERLLTTMQEKGKLLDFILCVGDDRSDEDMFEVIMSAKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLDGLAATNTTISDQTDSTATVPTKDLF

Target ORF information:

RefSeq Version	NM_117886.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana trehalose phosphatase/synthase 5 (TPS5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117886.3

ORF Insert Sequence:

ATGGTATCAA GATCTTATTC AAACCTCTTG GATCTTGCTT CTGGTAACTT CCATTCGTTT 
TCTCGAGAAA AGAAGAGGTT TCCAAGAGTA GCAACTGTCA CTGGTGTCTT ATCTGAGCTA 
GATGATGATA ACAACAGCAA CAGTGTCTGC TCTGATGCTC CTTCTTCCGT CACACAAGAT 
CGAATCATCA TCGTTGGAAA CCAGCTTCCT ATTAAATCAC ATCGGAACTC TGCTGGTAAA 
TTGAGTTTTA GTTGGGACAA TGACTCGCTT CTCTTGCAGC TTAAAGATGG TATGCGTGAA 
GATATGGAAG TTGTCTACAT TGGTTGCCTT AAGGAACAGA TTGATACAGT TGAGCAAGAT 
GATGTTTCTC AAAGGTTGCT TGAGAATTTC AAATGTGTTC CTGCTTATAT CCCACCTGAG 
CTATTCACTA AGTACTATCA TGGATTCTGT AAGCAACATC TATGGCCTTT GTTTCACTAC 
ATGCTTCCCT TAACTCCTGA TCTTGGTGGT AGATTTGATC GTTCTTTATG GCAAGCTTAT 
CTTTCGGTTA ACAAGATCTT TGCTGATAAA GTGATGGAAG TGATTAGTCC TGATGATGAT 
TTTGTTTGGG TTCATGACTA TCACTTAATG GTTTTGCCTA CATTTCTAAG GAAGAGGTTT 
AATAGAGTAA AGCTTGGTTT TTTCCTTCAT AGCCCGTTCC CTTCCTCTGA GATTTACCGT 
ACTCTTCCAG TGAGAAATGA GCTCTTACGT GCACTCCTCA ACGCTGATTT GATTGGCTTT 
CATACCTTTG ACTATGCAAG ACACTTCCTT TCTTGTTGTA GCAGGATGCT TGGTTTATCC 
TATCAGTCCA AACGTGGAAC CATAGGGCTT GAGTATTATG GTCGAACGGT TAGTATCAAG 
ATTCTTCCTG TGGGGATTCA TATCAGCCAG CTTCAGTCAA TTTTAAACCT CCCAGAGACT 
CAGACCAAAG TTGCTGAGCT AAGAGATCAG TTCTTGGATC AGAAAGTTCT TCTCGGTGTT 
GATGACATGG ACATCTTCAA AGGAATCAGC CTCAAACTCT TGGCAATGGA ACAACTTCTC 
ACACAGCATC CCGAGAAGAG AGGTCGAGTT GTACTTGTCC AGATTGCAAA CCCTGCAAGA 
GGTCGTGGGA AAGACGTTCA GGAGGTTCAG TCTGAAACTG AAGCCACGGT TAAAAGGATC 
AATGAAATGT TTGGAAGGCC GGGCTACCAA CCCGTGGTTC TGATTGATAC ACCGCTTCAA 
TTCTTTGAGA GGATTGCTTA CTATGTCATT GCAGAGTGTT GTCTTGTTAC AGCGGTAAGA 
GATGGTATGA ATCTTATACC TTATGAGTAC ATTATCTGCA GGCAAGGTAA TCCGAAACTC 
AACGAGACTA TAGGCCTTGA CCCTTCTGCT GCAAAGAAGA GTATGCTTGT TGTCTCTGAG 
TTTATTGGTT GTTCTCCTTC TTTAAGCGGC GCCATTAGAG TAAATCCGTG GAACATTGAT 
GCTGTGACTG AAGCAATGGA CTATGCATTG ATAGTTTCAG AAGCAGAGAA GCAAATGCGT 
CACGAGAAGC ATCACAAATA TGTTAGCACA CATGATGTTG CTTATTGGGC GCGTAGCTTT 
ATACAAGATC TTGAAAGGGC TTGCGGGGAT CATGTGAGGA AGAGGTGTTG GGGGATTGGA 
TTCGGGTTAG GCTTTAGAGT TGTGGCGCTT GATCCGAGTT TTAAAAAGCT TTCGATTGAG 
CACATTGTCT CAGCTTATAA GAGAACCAAG AACCGAGCCA TTTTGTTGGA TTATGATGGC 
ACAATGGTGC AGCCAGGTTC CATTAGGACA ACACCAACCC GCGAAACAAT CGAAATCTTG 
AACAACCTGT CTAGTGATCC CAAGAATATC GTGTACCTCG TCAGTGGGAA AGACAGAAGG 
ACACTAACTG AATGGTTTTC TTCATGTGAC GATCTCGGTT TGGGTGCAGA GCACGGGTAT 
TTTATAAGGC CAAATGATGG AACAGACTGG GAAACGTCGA GTTTGGTATC AGGTTTTGAG 
TGGAAACAAA TAGCAGAGCC AGTGATGAGA CTTTACACTG AGACTACAGA TGGATCAACA 
ATAGAGACTA AAGAGACTGC TCTCGTTTGG AATTACCAAT TCGCAGATCC TGATTTTGGA 
TCTTGTCAAG CCAAAGAGCT TATGGAACAC CTCGAAAGCG TGCTTACCAA TGATCCAGTC 
TCTGTCAAGA CTGGACAACA ACTCGTTGAA GTTAAACCAC AGGGTGTGAA CAAAGGTCTT 
GTGGCAGAGA GGCTTCTAAC AACGATGCAA GAAAAAGGGA AACTTTTGGA TTTCATTCTC 
TGCGTCGGTG ATGATCGGTC TGATGAGGAT ATGTTTGAGG TGATAATGAG TGCTAAAGAT 
GGTCCAGCTT TGTCTCCTGT GGCTGAGATA TTCGCTTGCA CCGTTGGACA AAAGCCAAGC 
AAAGCAAAAT ACTATTTAGA CGATACTGCG GAGATAATCA GAATGCTGGA CGGTCTAGCC 
GCTACCAACA CAACTATCTC TGATCAAACT GATTCAACCG CCACTGTTCC AACTAAAGAT 
CTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPS5 ( NM_001341241.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329809.1 Arabidopsis thaliana trehalose phosphatase/synthase 5 (TPS5), mRNA.

Price & Availability↑

CloneID	OAb15239
Clone ID Related Accession (Same CDS sequence)	NM_117886.3 , NM_001341241.1
Accession Version	NM_001341241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	trehalose phosphatase/synthase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGTATCAA GATCTTATTC AAACCTCTTG GATCTTGCTT CTGGTAACTT CCATTCGTTT 
TCTCGAGAAA AGAAGAGGTT TCCAAGAGTA GCAACTGTCA CTGGTGTCTT ATCTGAGCTA 
GATGATGATA ACAACAGCAA CAGTGTCTGC TCTGATGCTC CTTCTTCCGT CACACAAGAT 
CGAATCATCA TCGTTGGAAA CCAGCTTCCT ATTAAATCAC ATCGGAACTC TGCTGGTAAA 
TTGAGTTTTA GTTGGGACAA TGACTCGCTT CTCTTGCAGC TTAAAGATGG TATGCGTGAA 
GATATGGAAG TTGTCTACAT TGGTTGCCTT AAGGAACAGA TTGATACAGT TGAGCAAGAT 
GATGTTTCTC AAAGGTTGCT TGAGAATTTC AAATGTGTTC CTGCTTATAT CCCACCTGAG 
CTATTCACTA AGTACTATCA TGGATTCTGT AAGCAACATC TATGGCCTTT GTTTCACTAC 
ATGCTTCCCT TAACTCCTGA TCTTGGTGGT AGATTTGATC GTTCTTTATG GCAAGCTTAT 
CTTTCGGTTA ACAAGATCTT TGCTGATAAA GTGATGGAAG TGATTAGTCC TGATGATGAT 
TTTGTTTGGG TTCATGACTA TCACTTAATG GTTTTGCCTA CATTTCTAAG GAAGAGGTTT 
AATAGAGTAA AGCTTGGTTT TTTCCTTCAT AGCCCGTTCC CTTCCTCTGA GATTTACCGT 
ACTCTTCCAG TGAGAAATGA GCTCTTACGT GCACTCCTCA ACGCTGATTT GATTGGCTTT 
CATACCTTTG ACTATGCAAG ACACTTCCTT TCTTGTTGTA GCAGGATGCT TGGTTTATCC 
TATCAGTCCA AACGTGGAAC CATAGGGCTT GAGTATTATG GTCGAACGGT TAGTATCAAG 
ATTCTTCCTG TGGGGATTCA TATCAGCCAG CTTCAGTCAA TTTTAAACCT CCCAGAGACT 
CAGACCAAAG TTGCTGAGCT AAGAGATCAG TTCTTGGATC AGAAAGTTCT TCTCGGTGTT 
GATGACATGG ACATCTTCAA AGGAATCAGC CTCAAACTCT TGGCAATGGA ACAACTTCTC 
ACACAGCATC CCGAGAAGAG AGGTCGAGTT GTACTTGTCC AGATTGCAAA CCCTGCAAGA 
GGTCGTGGGA AAGACGTTCA GGAGGTTCAG TCTGAAACTG AAGCCACGGT TAAAAGGATC 
AATGAAATGT TTGGAAGGCC GGGCTACCAA CCCGTGGTTC TGATTGATAC ACCGCTTCAA 
TTCTTTGAGA GGATTGCTTA CTATGTCATT GCAGAGTGTT GTCTTGTTAC AGCGGTAAGA 
GATGGTATGA ATCTTATACC TTATGAGTAC ATTATCTGCA GGCAAGGTAA TCCGAAACTC 
AACGAGACTA TAGGCCTTGA CCCTTCTGCT GCAAAGAAGA GTATGCTTGT TGTCTCTGAG 
TTTATTGGTT GTTCTCCTTC TTTAAGCGGC GCCATTAGAG TAAATCCGTG GAACATTGAT 
GCTGTGACTG AAGCAATGGA CTATGCATTG ATAGTTTCAG AAGCAGAGAA GCAAATGCGT 
CACGAGAAGC ATCACAAATA TGTTAGCACA CATGATGTTG CTTATTGGGC GCGTAGCTTT 
ATACAAGATC TTGAAAGGGC TTGCGGGGAT CATGTGAGGA AGAGGTGTTG GGGGATTGGA 
TTCGGGTTAG GCTTTAGAGT TGTGGCGCTT GATCCGAGTT TTAAAAAGCT TTCGATTGAG 
CACATTGTCT CAGCTTATAA GAGAACCAAG AACCGAGCCA TTTTGTTGGA TTATGATGGC 
ACAATGGTGC AGCCAGGTTC CATTAGGACA ACACCAACCC GCGAAACAAT CGAAATCTTG 
AACAACCTGT CTAGTGATCC CAAGAATATC GTGTACCTCG TCAGTGGGAA AGACAGAAGG 
ACACTAACTG AATGGTTTTC TTCATGTGAC GATCTCGGTT TGGGTGCAGA GCACGGGTAT 
TTTATAAGGC CAAATGATGG AACAGACTGG GAAACGTCGA GTTTGGTATC AGGTTTTGAG 
TGGAAACAAA TAGCAGAGCC AGTGATGAGA CTTTACACTG AGACTACAGA TGGATCAACA 
ATAGAGACTA AAGAGACTGC TCTCGTTTGG AATTACCAAT TCGCAGATCC TGATTTTGGA 
TCTTGTCAAG CCAAAGAGCT TATGGAACAC CTCGAAAGCG TGCTTACCAA TGATCCAGTC 
TCTGTCAAGA CTGGACAACA ACTCGTTGAA GTTAAACCAC AGGGTGTGAA CAAAGGTCTT 
GTGGCAGAGA GGCTTCTAAC AACGATGCAA GAAAAAGGGA AACTTTTGGA TTTCATTCTC 
TGCGTCGGTG ATGATCGGTC TGATGAGGAT ATGTTTGAGG TGATAATGAG TGCTAAAGAT 
GGTCCAGCTT TGTCTCCTGT GGCTGAGATA TTCGCTTGCA CCGTTGGACA AAAGCCAAGC 
AAAGCAAAAT ACTATTTAGA CGATACTGCG GAGATAATCA GAATGCTGGA CGGTCTAGCC 
GCTACCAACA CAACTATCTC TGATCAAACT GATTCAACCG CCACTGTTCC AACTAAAGAT 
CTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329809.1
CDS	513..3101
Translation	MVSRSYSNLLDLASGNFHSFSREKKRFPRVATVTGVLSELDDDNNSNSVCSDAPSSVTQDRIIIVGNQLPIKSHRNSAGKLSFSWDNDSLLLQLKDGMREDMEVVYIGCLKEQIDTVEQDDVSQRLLENFKCVPAYIPPELFTKYYHGFCKQHLWPLFHYMLPLTPDLGGRFDRSLWQAYLSVNKIFADKVMEVISPDDDFVWVHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPSSEIYRTLPVRNELLRALLNADLIGFHTFDYARHFLSCCSRMLGLSYQSKRGTIGLEYYGRTVSIKILPVGIHISQLQSILNLPETQTKVAELRDQFLDQKVLLGVDDMDIFKGISLKLLAMEQLLTQHPEKRGRVVLVQIANPARGRGKDVQEVQSETEATVKRINEMFGRPGYQPVVLIDTPLQFFERIAYYVIAECCLVTAVRDGMNLIPYEYIICRQGNPKLNETIGLDPSAAKKSMLVVSEFIGCSPSLSGAIRVNPWNIDAVTEAMDYALIVSEAEKQMRHEKHHKYVSTHDVAYWARSFIQDLERACGDHVRKRCWGIGFGLGFRVVALDPSFKKLSIEHIVSAYKRTKNRAILLDYDGTMVQPGSIRTTPTRETIEILNNLSSDPKNIVYLVSGKDRRTLTEWFSSCDDLGLGAEHGYFIRPNDGTDWETSSLVSGFEWKQIAEPVMRLYTETTDGSTIETKETALVWNYQFADPDFGSCQAKELMEHLESVLTNDPVSVKTGQQLVEVKPQGVNKGLVAERLLTTMQEKGKLLDFILCVGDDRSDEDMFEVIMSAKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLDGLAATNTTISDQTDSTATVPTKDLF

Target ORF information:

RefSeq Version	NM_001341241.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana trehalose phosphatase/synthase 5 (TPS5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341241.1

ORF Insert Sequence:

ATGGTATCAA GATCTTATTC AAACCTCTTG GATCTTGCTT CTGGTAACTT CCATTCGTTT 
TCTCGAGAAA AGAAGAGGTT TCCAAGAGTA GCAACTGTCA CTGGTGTCTT ATCTGAGCTA 
GATGATGATA ACAACAGCAA CAGTGTCTGC TCTGATGCTC CTTCTTCCGT CACACAAGAT 
CGAATCATCA TCGTTGGAAA CCAGCTTCCT ATTAAATCAC ATCGGAACTC TGCTGGTAAA 
TTGAGTTTTA GTTGGGACAA TGACTCGCTT CTCTTGCAGC TTAAAGATGG TATGCGTGAA 
GATATGGAAG TTGTCTACAT TGGTTGCCTT AAGGAACAGA TTGATACAGT TGAGCAAGAT 
GATGTTTCTC AAAGGTTGCT TGAGAATTTC AAATGTGTTC CTGCTTATAT CCCACCTGAG 
CTATTCACTA AGTACTATCA TGGATTCTGT AAGCAACATC TATGGCCTTT GTTTCACTAC 
ATGCTTCCCT TAACTCCTGA TCTTGGTGGT AGATTTGATC GTTCTTTATG GCAAGCTTAT 
CTTTCGGTTA ACAAGATCTT TGCTGATAAA GTGATGGAAG TGATTAGTCC TGATGATGAT 
TTTGTTTGGG TTCATGACTA TCACTTAATG GTTTTGCCTA CATTTCTAAG GAAGAGGTTT 
AATAGAGTAA AGCTTGGTTT TTTCCTTCAT AGCCCGTTCC CTTCCTCTGA GATTTACCGT 
ACTCTTCCAG TGAGAAATGA GCTCTTACGT GCACTCCTCA ACGCTGATTT GATTGGCTTT 
CATACCTTTG ACTATGCAAG ACACTTCCTT TCTTGTTGTA GCAGGATGCT TGGTTTATCC 
TATCAGTCCA AACGTGGAAC CATAGGGCTT GAGTATTATG GTCGAACGGT TAGTATCAAG 
ATTCTTCCTG TGGGGATTCA TATCAGCCAG CTTCAGTCAA TTTTAAACCT CCCAGAGACT 
CAGACCAAAG TTGCTGAGCT AAGAGATCAG TTCTTGGATC AGAAAGTTCT TCTCGGTGTT 
GATGACATGG ACATCTTCAA AGGAATCAGC CTCAAACTCT TGGCAATGGA ACAACTTCTC 
ACACAGCATC CCGAGAAGAG AGGTCGAGTT GTACTTGTCC AGATTGCAAA CCCTGCAAGA 
GGTCGTGGGA AAGACGTTCA GGAGGTTCAG TCTGAAACTG AAGCCACGGT TAAAAGGATC 
AATGAAATGT TTGGAAGGCC GGGCTACCAA CCCGTGGTTC TGATTGATAC ACCGCTTCAA 
TTCTTTGAGA GGATTGCTTA CTATGTCATT GCAGAGTGTT GTCTTGTTAC AGCGGTAAGA 
GATGGTATGA ATCTTATACC TTATGAGTAC ATTATCTGCA GGCAAGGTAA TCCGAAACTC 
AACGAGACTA TAGGCCTTGA CCCTTCTGCT GCAAAGAAGA GTATGCTTGT TGTCTCTGAG 
TTTATTGGTT GTTCTCCTTC TTTAAGCGGC GCCATTAGAG TAAATCCGTG GAACATTGAT 
GCTGTGACTG AAGCAATGGA CTATGCATTG ATAGTTTCAG AAGCAGAGAA GCAAATGCGT 
CACGAGAAGC ATCACAAATA TGTTAGCACA CATGATGTTG CTTATTGGGC GCGTAGCTTT 
ATACAAGATC TTGAAAGGGC TTGCGGGGAT CATGTGAGGA AGAGGTGTTG GGGGATTGGA 
TTCGGGTTAG GCTTTAGAGT TGTGGCGCTT GATCCGAGTT TTAAAAAGCT TTCGATTGAG 
CACATTGTCT CAGCTTATAA GAGAACCAAG AACCGAGCCA TTTTGTTGGA TTATGATGGC 
ACAATGGTGC AGCCAGGTTC CATTAGGACA ACACCAACCC GCGAAACAAT CGAAATCTTG 
AACAACCTGT CTAGTGATCC CAAGAATATC GTGTACCTCG TCAGTGGGAA AGACAGAAGG 
ACACTAACTG AATGGTTTTC TTCATGTGAC GATCTCGGTT TGGGTGCAGA GCACGGGTAT 
TTTATAAGGC CAAATGATGG AACAGACTGG GAAACGTCGA GTTTGGTATC AGGTTTTGAG 
TGGAAACAAA TAGCAGAGCC AGTGATGAGA CTTTACACTG AGACTACAGA TGGATCAACA 
ATAGAGACTA AAGAGACTGC TCTCGTTTGG AATTACCAAT TCGCAGATCC TGATTTTGGA 
TCTTGTCAAG CCAAAGAGCT TATGGAACAC CTCGAAAGCG TGCTTACCAA TGATCCAGTC 
TCTGTCAAGA CTGGACAACA ACTCGTTGAA GTTAAACCAC AGGGTGTGAA CAAAGGTCTT 
GTGGCAGAGA GGCTTCTAAC AACGATGCAA GAAAAAGGGA AACTTTTGGA TTTCATTCTC 
TGCGTCGGTG ATGATCGGTC TGATGAGGAT ATGTTTGAGG TGATAATGAG TGCTAAAGAT 
GGTCCAGCTT TGTCTCCTGT GGCTGAGATA TTCGCTTGCA CCGTTGGACA AAAGCCAAGC 
AAAGCAAAAT ACTATTTAGA CGATACTGCG GAGATAATCA GAATGCTGGA CGGTCTAGCC 
GCTACCAACA CAACTATCTC TGATCAAACT GATTCAACCG CCACTGTTCC AACTAAAGAT 
CTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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TPS5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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