NIG cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIG
Entrez Gene ID	826966
Full Name	NSP (nuclear shuttle protein)-interacting GTPase
Synonyms	NSP (nuclear shuttle protein)-interacting GTPase,T9E8.90,T9E8_90
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_117409.5	NP_193071.2	NSP (nuclear shuttle protein)-interacting GTPase
NM_179209.2	NP_849540.1	NSP (nuclear shuttle protein)-interacting GTPase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0208900	NP_001046262.1
Arabidopsis thaliana (thale cress)	NIG	NP_849540.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NIG protein is a GTPase that interacts with geminivirus nuclear shuttle protein. NIG increases the nuclear export rates of viral NSP which contributes to geminivirus infection. [NIG]
Title: A novel nucleocytoplasmic traffic GTPase identified as a functional target of the bipartite geminivirus nuclear shuttle protein.

The following NIG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb30645	NM_179209.2 Latest version!	Arabidopsis thaliana NSP (nuclear shuttle protein)-interacting GTPase (NIG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb30645	NM_117409.5 Latest version!	Arabidopsis thaliana NSP (nuclear shuttle protein)-interacting GTPase (NIG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIG ( NM_117409.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193071.2 Arabidopsis thaliana NSP (nuclear shuttle protein)-interacting GTPase (NIG), mRNA.

Price & Availability↑

CloneID	OAb30645
Clone ID Related Accession (Same CDS sequence)	NM_117409.5 , NM_179209.2
Accession Version	NM_117409.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NSP (nuclear shuttle protein)-interacting GTPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_117409.4.

ATGGCGGGTC GAGTTAAAGA AGACGAGAAG AACGAGAAGA TTATTAGGAG TCTTCTTAAA 
CTTCCTGAGA ATAAGAGGTG TATAAACTGT AACAGCCTTG GACCACAATA TGTTTGCACT 
ACTTTCTGGA CTTTTGTTTG TACCAACTGC AGTGGAATAC ATCGTGAGTT TACACATCGT 
GTTAAATCGA TATCAATGGC GAAATTTACC TCACAAGAAG TTACTGCTTT GAAAGAAGGT 
GGAAATCAGC ATGCTAAGGA TATTTATTTT AAAGGATTGG ATCAACAGCG GCAATCAGTG 
CCTGACGGAA GTAATGTTGA GCGGTTAAGG GACTTCATCA GACATGTGTA TGTAAATAAA 
AGGTATACGA ATGAGAAGAA TGATGATAAG TCTCCAAGTG AGACACGTAG TTCTAGTGGT 
TCTCGGAGTC CACCTTATGA AGATGGATAT GACCGTCGTT ACGGCGACAG ATCAAGTCCT 
GGTGGGAGAA GTCCAGGGTT CGAGACGGGT AGTAGGAATG CTGTTAATAA CAGAAAAAGC 
CCTGCTCGTC CTGAGATCCT GAATGATTGG CGCAGAGAGG ATAGGTTTGG GGGTAGAAAA 
ACATCAGAAG AGGGCTCCCA GTCACCTGAG CAAGTAAAAG ACTTGGGTTC AGCTAGCCCT 
CCTGTAGCTC GACCTGTTAG GGAAATTTTG GGTGACAGTG TTATTCCTCT TCGTGTGGGA 
GAGCCTCCAA AACCCCCTGT CAGTAGAAAT ACCGATGCTT CAGCACATGC AAAGTCAGGT 
ACATCATTGA GTAGTTTAAT GTCCACAAAT GAGAAGCCAC CAGAAGTGAA GCTGGAGACT 
GCTTTAAGCC TGATTGATTT TGATACTGAT TTTGAAACTC CTGCTCCATC TGTTGCAATT 
CAAGCACCAC TATCGACCAC ATCTCAGCCT GCTCCACAGC CAACAACTTC ATCTAATGAT 
AACTGGGCAT CTTTCGATGC TGCACCCAGT GCCCCTAGTT TAAATGTATC GCAACCACCA 
CCCAGTGGAA ACACACTGGA TTCACTTCTC TCCCAGTTGG CAGTGACTTC TTCTGTGCCA 
GGTCAGGCGT CTACACCATC TAACGGACCT GTGAATCTTG GTCATTCCAC GTCACAAATC 
TTTGCACCCT TTCAGAATGA GCATTCGAGT GAACAGCCAT GGAACACAGC ACTTGCATCT 
AATGTACAGA GGTCAATGAG TGCACCGTCA TTGCAACCTC TTCAAGGAGT CCCTTCTGGT 
GGCCTGCAGT CTTCTGAAGT TAAACCTTCG GGAAGATCAG AATTACCTGC GGATTTATTC 
GCTGTAAACT ACCCATCATA CCATGCACCA GTGCCTGGAT GGCAAGCTGG TCCACCTCAT 
GCTATGCACT ATGGCATGCA GCAGTATAAT AACCCCGTGC CGTATCAGAA TGTTCCGCAG 
CCAGGCAAGT CAATGAACCC TTTTGACTTC AGCCCTGGGC CGCCATCACA AACACAAACG 
GAAAATATGT TCCCTTCCAT GGCTCCTCTG CAAGGTGCAT TACCTCCTTC AGGCATGATG 
CCTTCTCAAG GAGTACACAA TCAATTCAAT ATACCTTCTC AAGGCTCAGC CCATCCATCT 
GCAATGCCAC CAAGGTACAT GCCTTCTCAA ATACCAGGAA GCATGCCACC TAGCAATGTA 
AACCCAATTG GCGACATAAA CACGTCATAT GACACTCAAC AAACATATCA AAATTTTGGA 
AGCTCGTTTG CTGCTGCAGT TCCTTTGAAC CCACCTTCTT TCCAATCAGG AGGAAACCCA 
TTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193071.2
CDS	283..2091
Translation	MAGRVKEDEKNEKIIRSLLKLPENKRCINCNSLGPQYVCTTFWTFVCTNCSGIHREFTHRVKSISMAKFTSQEVTALKEGGNQHAKDIYFKGLDQQRQSVPDGSNVERLRDFIRHVYVNKRYTNEKNDDKSPSETRSSSGSRSPPYEDGYDRRYGDRSSPGGRSPGFETGSRNAVNNRKSPARPEILNDWRREDRFGGRKTSEEGSQSPEQVKDLGSASPPVARPVREILGDSVIPLRVGEPPKPPVSRNTDASAHAKSGTSLSSLMSTNEKPPEVKLETALSLIDFDTDFETPAPSVAIQAPLSTTSQPAPQPTTSSNDNWASFDAAPSAPSLNVSQPPPSGNTLDSLLSQLAVTSSVPGQASTPSNGPVNLGHSTSQIFAPFQNEHSSEQPWNTALASNVQRSMSAPSLQPLQGVPSGGLQSSEVKPSGRSELPADLFAVNYPSYHAPVPGWQAGPPHAMHYGMQQYNNPVPYQNVPQPGKSMNPFDFSPGPPSQTQTENMFPSMAPLQGALPPSGMMPSQGVHNQFNIPSQGSAHPSAMPPRYMPSQIPGSMPPSNVNPIGDINTSYDTQQTYQNFGSSFAAAVPLNPPSFQSGGNPFG

Target ORF information:

RefSeq Version	NM_117409.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NSP (nuclear shuttle protein)-interacting GTPase (NIG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117409.5

ORF Insert Sequence:

ATGGCGGGTC GAGTTAAAGA AGACGAGAAG AACGAGAAGA TTATTAGGAG TCTTCTTAAA 
CTTCCTGAGA ATAAGAGGTG TATAAACTGT AACAGCCTTG GACCACAATA TGTTTGCACT 
ACTTTCTGGA CTTTTGTTTG TACCAACTGC AGTGGAATAC ATCGTGAGTT TACACATCGT 
GTTAAATCGA TATCAATGGC GAAATTTACC TCACAAGAAG TTACTGCTTT GAAAGAAGGT 
GGAAATCAGC ATGCTAAGGA TATTTATTTT AAAGGATTGG ATCAACAGCG GCAATCAGTG 
CCTGACGGAA GTAATGTTGA GCGGTTAAGG GACTTCATCA GACATGTGTA TGTAAATAAA 
AGGTATACGA ATGAGAAGAA TGATGATAAG TCTCCAAGTG AGACACGTAG TTCTAGTGGT 
TCTCGGAGTC CACCTTATGA AGATGGATAT GACCGTCGTT ACGGCGACAG ATCAAGTCCT 
GGTGGGAGAA GTCCAGGGTT CGAGACGGGT AGTAGGAATG CTGTTAATAA CAGAAAAAGC 
CCTGCTCGTC CTGAGATCCT GAATGATTGG CGCAGAGAGG ATAGGTTTGG GGGTAGAAAA 
ACATCAGAAG AGGGCTCCCA GTCACCTGAG CAAGTAAAAG ACTTGGGTTC AGCTAGCCCT 
CCTGTAGCTC GACCTGTTAG GGAAATTTTG GGTGACAGTG TTATTCCTCT TCGTGTGGGA 
GAGCCTCCAA AACCCCCTGT CAGTAGAAAT ACCGATGCTT CAGCACATGC AAAGTCAGGT 
ACATCATTGA GTAGTTTAAT GTCCACAAAT GAGAAGCCAC CAGAAGTGAA GCTGGAGACT 
GCTTTAAGCC TGATTGATTT TGATACTGAT TTTGAAACTC CTGCTCCATC TGTTGCAATT 
CAAGCACCAC TATCGACCAC ATCTCAGCCT GCTCCACAGC CAACAACTTC ATCTAATGAT 
AACTGGGCAT CTTTCGATGC TGCACCCAGT GCCCCTAGTT TAAATGTATC GCAACCACCA 
CCCAGTGGAA ACACACTGGA TTCACTTCTC TCCCAGTTGG CAGTGACTTC TTCTGTGCCA 
GGTCAGGCGT CTACACCATC TAACGGACCT GTGAATCTTG GTCATTCCAC GTCACAAATC 
TTTGCACCCT TTCAGAATGA GCATTCGAGT GAACAGCCAT GGAACACAGC ACTTGCATCT 
AATGTACAGA GGTCAATGAG TGCACCGTCA TTGCAACCTC TTCAAGGAGT CCCTTCTGGT 
GGCCTGCAGT CTTCTGAAGT TAAACCTTCG GGAAGATCAG AATTACCTGC GGATTTATTC 
GCTGTAAACT ACCCATCATA CCATGCACCA GTGCCTGGAT GGCAAGCTGG TCCACCTCAT 
GCTATGCACT ATGGCATGCA GCAGTATAAT AACCCCGTGC CGTATCAGAA TGTTCCGCAG 
CCAGGCAAGT CAATGAACCC TTTTGACTTC AGCCCTGGGC CGCCATCACA AACACAAACG 
GAAAATATGT TCCCTTCCAT GGCTCCTCTG CAAGGTGCAT TACCTCCTTC AGGCATGATG 
CCTTCTCAAG GAGTACACAA TCAATTCAAT ATACCTTCTC AAGGCTCAGC CCATCCATCT 
GCAATGCCAC CAAGGTACAT GCCTTCTCAA ATACCAGGAA GCATGCCACC TAGCAATGTA 
AACCCAATTG GCGACATAAA CACGTCATAT GACACTCAAC AAACATATCA AAATTTTGGA 
AGCTCGTTTG CTGCTGCAGT TCCTTTGAAC CCACCTTCTT TCCAATCAGG AGGAAACCCA 
TTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIG ( NM_179209.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849540.1 Arabidopsis thaliana NSP (nuclear shuttle protein)-interacting GTPase (NIG), mRNA.

Price & Availability↑

CloneID	OAb30645
Clone ID Related Accession (Same CDS sequence)	NM_117409.5 , NM_179209.2
Accession Version	NM_179209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NSP (nuclear shuttle protein)-interacting GTPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 18, 2007 this sequence version replaced NM_179209.1.

ATGGCGGGTC GAGTTAAAGA AGACGAGAAG AACGAGAAGA TTATTAGGAG TCTTCTTAAA 
CTTCCTGAGA ATAAGAGGTG TATAAACTGT AACAGCCTTG GACCACAATA TGTTTGCACT 
ACTTTCTGGA CTTTTGTTTG TACCAACTGC AGTGGAATAC ATCGTGAGTT TACACATCGT 
GTTAAATCGA TATCAATGGC GAAATTTACC TCACAAGAAG TTACTGCTTT GAAAGAAGGT 
GGAAATCAGC ATGCTAAGGA TATTTATTTT AAAGGATTGG ATCAACAGCG GCAATCAGTG 
CCTGACGGAA GTAATGTTGA GCGGTTAAGG GACTTCATCA GACATGTGTA TGTAAATAAA 
AGGTATACGA ATGAGAAGAA TGATGATAAG TCTCCAAGTG AGACACGTAG TTCTAGTGGT 
TCTCGGAGTC CACCTTATGA AGATGGATAT GACCGTCGTT ACGGCGACAG ATCAAGTCCT 
GGTGGGAGAA GTCCAGGGTT CGAGACGGGT AGTAGGAATG CTGTTAATAA CAGAAAAAGC 
CCTGCTCGTC CTGAGATCCT GAATGATTGG CGCAGAGAGG ATAGGTTTGG GGGTAGAAAA 
ACATCAGAAG AGGGCTCCCA GTCACCTGAG CAAGTAAAAG ACTTGGGTTC AGCTAGCCCT 
CCTGTAGCTC GACCTGTTAG GGAAATTTTG GGTGACAGTG TTATTCCTCT TCGTGTGGGA 
GAGCCTCCAA AACCCCCTGT CAGTAGAAAT ACCGATGCTT CAGCACATGC AAAGTCAGGT 
ACATCATTGA GTAGTTTAAT GTCCACAAAT GAGAAGCCAC CAGAAGTGAA GCTGGAGACT 
GCTTTAAGCC TGATTGATTT TGATACTGAT TTTGAAACTC CTGCTCCATC TGTTGCAATT 
CAAGCACCAC TATCGACCAC ATCTCAGCCT GCTCCACAGC CAACAACTTC ATCTAATGAT 
AACTGGGCAT CTTTCGATGC TGCACCCAGT GCCCCTAGTT TAAATGTATC GCAACCACCA 
CCCAGTGGAA ACACACTGGA TTCACTTCTC TCCCAGTTGG CAGTGACTTC TTCTGTGCCA 
GGTCAGGCGT CTACACCATC TAACGGACCT GTGAATCTTG GTCATTCCAC GTCACAAATC 
TTTGCACCCT TTCAGAATGA GCATTCGAGT GAACAGCCAT GGAACACAGC ACTTGCATCT 
AATGTACAGA GGTCAATGAG TGCACCGTCA TTGCAACCTC TTCAAGGAGT CCCTTCTGGT 
GGCCTGCAGT CTTCTGAAGT TAAACCTTCG GGAAGATCAG AATTACCTGC GGATTTATTC 
GCTGTAAACT ACCCATCATA CCATGCACCA GTGCCTGGAT GGCAAGCTGG TCCACCTCAT 
GCTATGCACT ATGGCATGCA GCAGTATAAT AACCCCGTGC CGTATCAGAA TGTTCCGCAG 
CCAGGCAAGT CAATGAACCC TTTTGACTTC AGCCCTGGGC CGCCATCACA AACACAAACG 
GAAAATATGT TCCCTTCCAT GGCTCCTCTG CAAGGTGCAT TACCTCCTTC AGGCATGATG 
CCTTCTCAAG GAGTACACAA TCAATTCAAT ATACCTTCTC AAGGCTCAGC CCATCCATCT 
GCAATGCCAC CAAGGTACAT GCCTTCTCAA ATACCAGGAA GCATGCCACC TAGCAATGTA 
AACCCAATTG GCGACATAAA CACGTCATAT GACACTCAAC AAACATATCA AAATTTTGGA 
AGCTCGTTTG CTGCTGCAGT TCCTTTGAAC CCACCTTCTT TCCAATCAGG AGGAAACCCA 
TTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849540.1
CDS	131..1939
Translation	MAGRVKEDEKNEKIIRSLLKLPENKRCINCNSLGPQYVCTTFWTFVCTNCSGIHREFTHRVKSISMAKFTSQEVTALKEGGNQHAKDIYFKGLDQQRQSVPDGSNVERLRDFIRHVYVNKRYTNEKNDDKSPSETRSSSGSRSPPYEDGYDRRYGDRSSPGGRSPGFETGSRNAVNNRKSPARPEILNDWRREDRFGGRKTSEEGSQSPEQVKDLGSASPPVARPVREILGDSVIPLRVGEPPKPPVSRNTDASAHAKSGTSLSSLMSTNEKPPEVKLETALSLIDFDTDFETPAPSVAIQAPLSTTSQPAPQPTTSSNDNWASFDAAPSAPSLNVSQPPPSGNTLDSLLSQLAVTSSVPGQASTPSNGPVNLGHSTSQIFAPFQNEHSSEQPWNTALASNVQRSMSAPSLQPLQGVPSGGLQSSEVKPSGRSELPADLFAVNYPSYHAPVPGWQAGPPHAMHYGMQQYNNPVPYQNVPQPGKSMNPFDFSPGPPSQTQTENMFPSMAPLQGALPPSGMMPSQGVHNQFNIPSQGSAHPSAMPPRYMPSQIPGSMPPSNVNPIGDINTSYDTQQTYQNFGSSFAAAVPLNPPSFQSGGNPFG

Target ORF information:

RefSeq Version	NM_179209.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NSP (nuclear shuttle protein)-interacting GTPase (NIG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179209.2

ORF Insert Sequence:

ATGGCGGGTC GAGTTAAAGA AGACGAGAAG AACGAGAAGA TTATTAGGAG TCTTCTTAAA 
CTTCCTGAGA ATAAGAGGTG TATAAACTGT AACAGCCTTG GACCACAATA TGTTTGCACT 
ACTTTCTGGA CTTTTGTTTG TACCAACTGC AGTGGAATAC ATCGTGAGTT TACACATCGT 
GTTAAATCGA TATCAATGGC GAAATTTACC TCACAAGAAG TTACTGCTTT GAAAGAAGGT 
GGAAATCAGC ATGCTAAGGA TATTTATTTT AAAGGATTGG ATCAACAGCG GCAATCAGTG 
CCTGACGGAA GTAATGTTGA GCGGTTAAGG GACTTCATCA GACATGTGTA TGTAAATAAA 
AGGTATACGA ATGAGAAGAA TGATGATAAG TCTCCAAGTG AGACACGTAG TTCTAGTGGT 
TCTCGGAGTC CACCTTATGA AGATGGATAT GACCGTCGTT ACGGCGACAG ATCAAGTCCT 
GGTGGGAGAA GTCCAGGGTT CGAGACGGGT AGTAGGAATG CTGTTAATAA CAGAAAAAGC 
CCTGCTCGTC CTGAGATCCT GAATGATTGG CGCAGAGAGG ATAGGTTTGG GGGTAGAAAA 
ACATCAGAAG AGGGCTCCCA GTCACCTGAG CAAGTAAAAG ACTTGGGTTC AGCTAGCCCT 
CCTGTAGCTC GACCTGTTAG GGAAATTTTG GGTGACAGTG TTATTCCTCT TCGTGTGGGA 
GAGCCTCCAA AACCCCCTGT CAGTAGAAAT ACCGATGCTT CAGCACATGC AAAGTCAGGT 
ACATCATTGA GTAGTTTAAT GTCCACAAAT GAGAAGCCAC CAGAAGTGAA GCTGGAGACT 
GCTTTAAGCC TGATTGATTT TGATACTGAT TTTGAAACTC CTGCTCCATC TGTTGCAATT 
CAAGCACCAC TATCGACCAC ATCTCAGCCT GCTCCACAGC CAACAACTTC ATCTAATGAT 
AACTGGGCAT CTTTCGATGC TGCACCCAGT GCCCCTAGTT TAAATGTATC GCAACCACCA 
CCCAGTGGAA ACACACTGGA TTCACTTCTC TCCCAGTTGG CAGTGACTTC TTCTGTGCCA 
GGTCAGGCGT CTACACCATC TAACGGACCT GTGAATCTTG GTCATTCCAC GTCACAAATC 
TTTGCACCCT TTCAGAATGA GCATTCGAGT GAACAGCCAT GGAACACAGC ACTTGCATCT 
AATGTACAGA GGTCAATGAG TGCACCGTCA TTGCAACCTC TTCAAGGAGT CCCTTCTGGT 
GGCCTGCAGT CTTCTGAAGT TAAACCTTCG GGAAGATCAG AATTACCTGC GGATTTATTC 
GCTGTAAACT ACCCATCATA CCATGCACCA GTGCCTGGAT GGCAAGCTGG TCCACCTCAT 
GCTATGCACT ATGGCATGCA GCAGTATAAT AACCCCGTGC CGTATCAGAA TGTTCCGCAG 
CCAGGCAAGT CAATGAACCC TTTTGACTTC AGCCCTGGGC CGCCATCACA AACACAAACG 
GAAAATATGT TCCCTTCCAT GGCTCCTCTG CAAGGTGCAT TACCTCCTTC AGGCATGATG 
CCTTCTCAAG GAGTACACAA TCAATTCAAT ATACCTTCTC AAGGCTCAGC CCATCCATCT 
GCAATGCCAC CAAGGTACAT GCCTTCTCAA ATACCAGGAA GCATGCCACC TAGCAATGTA 
AACCCAATTG GCGACATAAA CACGTCATAT GACACTCAAC AAACATATCA AAATTTTGGA 
AGCTCGTTTG CTGCTGCAGT TCCTTTGAAC CCACCTTCTT TCCAATCAGG AGGAAACCCA 
TTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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NIG cDNA ORF clone, Arabidopsis thaliana(thale cress)

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