GUN1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUN1
Entrez Gene ID	817698
Full Name	s uncoupled 1
Synonyms	T28P16.11,T28P16_11,genomes uncoupled 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001336330.1	NP_001324971.1	s uncoupled 1
NM_128697.3	NP_180698.1	s uncoupled 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	GUN1	NP_180698.1
Oryza sativa (rice)	Os12g0562300	NP_001067027.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LIN treatment rapidly increases the levels of aggregated proteins in the chloroplast, unleashing a specific retrograde signaling pathway that up-regulates expression of ClpB3 and other nuclear genes encoding plastidial chaperones.Expression of HsfA2 and cpUPR-related target genes is independent of GUN1, a central integrator of retrograde signaling pathways
Title: Interference with plastome gene expression and Clp protease activity in Arabidopsis triggers a chloroplast unfolded protein response to restore protein homeostasis.

Defects in GUN1 or mTERF4 de-repressed the expression of specific plastid mRNAs in the presence of lincomycin (LIN). In wild-type plants, treatment with LIN or spectinomycin (SPE) inhibited processing of plastid transcripts.
Title: Functional relationship between mTERF4 and GUN1 in retrograde signaling.

Data show that the the genomes uncoupled 1 (gun1) mutation genetically interacts with mutations of genes for the chloroplast ribosomal proteins S1 (PRPS1).
Title: GUN1 Controls Accumulation of the Plastid Ribosomal Protein S1 at the Protein Level and Interacts with Proteins Involved in Plastid Protein Homeostasis.

GUN1 mediated plastid sugar metabolism is involved in nuclear gene expression regulation.
Title: A gain-of-function mutation of plastidic invertase alters nuclear gene expression with sucrose treatment partially via GENOMES UNCOUPLED1-mediated signaling.

GUN1 and GUN4 partially restored the expression patterns of the previously detected chloroplast-associated genes, thereby ameliorating the slow-greening phenotype of sg1.
Title: The tetratricopeptide repeat-containing protein slow green1 is required for chloroplast development in Arabidopsis.

The following GUN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb08453	NM_128697.3 Latest version!	Arabidopsis thaliana s uncoupled 1 (GUN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb36197	NM_001336330.1 Latest version!	Arabidopsis thaliana s uncoupled 1 (GUN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUN1 ( NM_128697.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_180698.1 Arabidopsis thaliana s uncoupled 1 (GUN1), mRNA.

Price & Availability↑

CloneID	OAb08453
Clone ID Related Accession (Same CDS sequence)	NM_128697.3
Accession Version	NM_128697.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	s uncoupled 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_128697.2.

ATGGCGTCAA CGCCGCCTCA CTGGGTCACC ACCACCAACA ACCACCGTCC ATGGCTTCCT 
CAGCGTCCTC GTCCCGGTCG CTCCGTTACC TCAGCTCCTC CCTCTTCTTC AGCCTCTGTT 
TCTTCAGCTC ATCTTTCACA GACTACTCCA AATTTCTCTC CTTTGCAAAC CCCCAAATCA 
GATTTCTCCG GTCGTCAATC TACCCGATTC GTCTCCCCCG CCACCAATAA CCACCGTCAG 
ACCAGGCAAA ACCCAAACTA CAACCACCGT CCGTATGGTG CCTCTTCTTC TCCTCGTGGC 
TCAGCTCCTC CGCCTTCATC TGTAGCTACT GTAGCTCCAG CTCAACTATC ACAACCTCCT 
AACTTTTCGC CTCTCCAAAC CCCAAAATCC GATCTTTCAT CGGATTTTTC AGGTAGACGA 
TCGACTAGAT TCGTCTCCAA GATGCATTTC GGGCGGCAGA AGACGACAAT GGCTACGAGA 
CATAGCTCTG CTGCTGAAGA TGCACTTCAA AACGCTATTG ATTTCTCTGG AGACGATGAG 
ATGTTTCATA GTCTAATGTT GAGCTTTGAG TCAAAGCTGT GTGGCTCTGA CGACTGTACC 
TATATAATCC GTGAGTTAGG GAATCGTAAT GAGTGCGATA AAGCTGTTGG CTTTTATGAG 
TTTGCGGTTA AACGAGAGAG GAGAAAGAAC GAGCAGGGGA AGTTAGCTAG TGCTATGATA 
AGTACTCTTG GTAGATATGG TAAAGTAACT ATTGCTAAGA GGATTTTCGA AACTGCTTTT 
GCTGGTGGAT ATGGAAACAC GGTTTACGCC TTCTCGGCTT TGATCAGCGC TTATGGGAGA 
AGTGGGCTAC ATGAGGAAGC CATTAGTGTC TTCAACTCCA TGAAAGAGTA TGGCTTGAGG 
CCGAATTTGG TTACCTACAA TGCTGTGATT GACGCTTGCG GTAAAGGCGG GATGGAGTTT 
AAGCAAGTAG CCAAGTTTTT CGATGAGATG CAGAGGAACG GTGTGCAGCC AGATAGAATA 
ACTTTCAACT CGTTGCTTGC TGTTTGCAGT AGAGGAGGGT TGTGGGAAGC AGCAAGGAAT 
CTGTTTGATG AGATGACGAA TAGAAGGATT GAGCAAGATG TTTTTTCTTA TAATACGCTT 
TTGGATGCAA TTTGCAAAGG TGGGCAGATG GACCTTGCTT TCGAAATCCT TGCTCAGATG 
CCAGTTAAGA GAATCATGCC TAATGTTGTA AGTTATAGCA CCGTTATTGA TGGGTTTGCA 
AAAGCCGGAA GATTTGATGA AGCTCTTAAC TTGTTTGGTG AAATGAGATA TCTTGGTATT 
GCATTAGATA GAGTTTCTTA CAATACATTG CTTTCAATCT ACACTAAAGT TGGTAGATCT 
GAAGAAGCTT TGGATATTCT GAGAGAAATG GCATCTGTTG GGATTAAAAA GGATGTTGTG 
ACTTATAATG CTCTTCTTGG AGGATATGGG AAACAAGGGA AATATGATGA AGTGAAGAAA 
GTTTTCACTG AGATGAAGCG AGAGCATGTG TTGCCTAACT TACTGACTTA TTCAACTTTG 
ATCGATGGGT ACTCGAAGGG GGGTTTGTAT AAAGAGGCAA TGGAGATATT TAGGGAGTTT 
AAGAGCGCCG GATTAAGAGC TGATGTTGTT TTGTACAGTG CATTGATTGA TGCATTGTGC 
AAGAATGGGT TGGTGGGTTC TGCTGTTTCT TTGATCGATG AGATGACAAA GGAAGGAATT 
AGTCCTAATG TTGTAACTTA TAATTCCATA ATCGATGCCT TTGGTCGTTC TGCAACTATG 
GACAGATCAG CCGATTATAG CAATGGTGGA TCTTTGCCTT TCTCTTCTTC TGCTCTGAGT 
GCATTGACTG AAACAGAGGG TAATCGGGTA ATACAGTTAT TTGGGCAGTT AACAACTGAG 
AGTAACAACC GAACGACGAA AGATTGTGAG GAGGGTATGC AGGAGCTCTC CTGTATATTG 
GAAGTTTTCC GCAAAATGCA TCAGCTGGAA ATTAAACCAA ATGTTGTAAC CTTTTCAGCG 
ATTCTGAATG CTTGCAGTCG GTGTAACTCA TTTGAAGATG CATCAATGCT ACTAGAGGAG 
CTCAGGCTGT TTGATAATAA GGTATATGGT GTTGTTCATG GACTTCTCAT GGGCCAGAGA 
GAGAATGTAT GGCTCCAAGC TCAGAGCTTA TTTGACAAAG TAAATGAAAT GGATGGTTCG 
ACTGCTTCTG CCTTCTACAA TGCCCTAACT GACATGCTTT GGCACTTTGG CCAGAAACGA 
GGTGCAGAAC TGGTGGCACT TGAGGGAAGA TCTAGGCAAG TTTGGGAAAA CGTGTGGTCT 
GATTCTTGCT TGGACTTACA CCTCATGTCT TCTGGAGCTG CACGTGCAAT GGTCCATGCG 
TGGCTACTAA ACATACGCTC CATTGTCTAT GAAGGTCATG AGTTACCTAA AGTCTTGAGT 
ATATTGACTG GCTGGGGGAA ACACAGCAAA GTGGTTGGAG ACGGAGCACT AAGACGAGCG 
GTTGAAGTTC TTTTGAGGGG AATGGATGCA CCATTCCACC TGTCAAAATG CAACATGGGA 
CGGTTCACAT CAAGCGGTTC GGTGGTAGCA ACTTGGCTGC GTGAATCAGC CACACTGAAA 
CTCTTAATCC TCCATGACCA CATAACAACC GCCACAGCTA CTACTACAAC AATGAAATCA 
ACAGACCAAC AACAACGGAA ACAGACGTCG TTTGCTTTAC AGCCTCTTCT TTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_180698.1
CDS	305..3061
Translation	MASTPPHWVTTTNNHRPWLPQRPRPGRSVTSAPPSSSASVSSAHLSQTTPNFSPLQTPKSDFSGRQSTRFVSPATNNHRQTRQNPNYNHRPYGASSSPRGSAPPPSSVATVAPAQLSQPPNFSPLQTPKSDLSSDFSGRRSTRFVSKMHFGRQKTTMATRHSSAAEDALQNAIDFSGDDEMFHSLMLSFESKLCGSDDCTYIIRELGNRNECDKAVGFYEFAVKRERRKNEQGKLASAMISTLGRYGKVTIAKRIFETAFAGGYGNTVYAFSALISAYGRSGLHEEAISVFNSMKEYGLRPNLVTYNAVIDACGKGGMEFKQVAKFFDEMQRNGVQPDRITFNSLLAVCSRGGLWEAARNLFDEMTNRRIEQDVFSYNTLLDAICKGGQMDLAFEILAQMPVKRIMPNVVSYSTVIDGFAKAGRFDEALNLFGEMRYLGIALDRVSYNTLLSIYTKVGRSEEALDILREMASVGIKKDVVTYNALLGGYGKQGKYDEVKKVFTEMKREHVLPNLLTYSTLIDGYSKGGLYKEAMEIFREFKSAGLRADVVLYSALIDALCKNGLVGSAVSLIDEMTKEGISPNVVTYNSIIDAFGRSATMDRSADYSNGGSLPFSSSALSALTETEGNRVIQLFGQLTTESNNRTTKDCEEGMQELSCILEVFRKMHQLEIKPNVVTFSAILNACSRCNSFEDASMLLEELRLFDNKVYGVVHGLLMGQRENVWLQAQSLFDKVNEMDGSTASAFYNALTDMLWHFGQKRGAELVALEGRSRQVWENVWSDSCLDLHLMSSGAARAMVHAWLLNIRSIVYEGHELPKVLSILTGWGKHSKVVGDGALRRAVEVLLRGMDAPFHLSKCNMGRFTSSGSVVATWLRESATLKLLILHDHITTATATTTTMKSTDQQQRKQTSFALQPLLL

Target ORF information:

RefSeq Version	NM_128697.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana s uncoupled 1 (GUN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_128697.3

ORF Insert Sequence:

ATGGCGTCAA CGCCGCCTCA CTGGGTCACC ACCACCAACA ACCACCGTCC ATGGCTTCCT 
CAGCGTCCTC GTCCCGGTCG CTCCGTTACC TCAGCTCCTC CCTCTTCTTC AGCCTCTGTT 
TCTTCAGCTC ATCTTTCACA GACTACTCCA AATTTCTCTC CTTTGCAAAC CCCCAAATCA 
GATTTCTCCG GTCGTCAATC TACCCGATTC GTCTCCCCCG CCACCAATAA CCACCGTCAG 
ACCAGGCAAA ACCCAAACTA CAACCACCGT CCGTATGGTG CCTCTTCTTC TCCTCGTGGC 
TCAGCTCCTC CGCCTTCATC TGTAGCTACT GTAGCTCCAG CTCAACTATC ACAACCTCCT 
AACTTTTCGC CTCTCCAAAC CCCAAAATCC GATCTTTCAT CGGATTTTTC AGGTAGACGA 
TCGACTAGAT TCGTCTCCAA GATGCATTTC GGGCGGCAGA AGACGACAAT GGCTACGAGA 
CATAGCTCTG CTGCTGAAGA TGCACTTCAA AACGCTATTG ATTTCTCTGG AGACGATGAG 
ATGTTTCATA GTCTAATGTT GAGCTTTGAG TCAAAGCTGT GTGGCTCTGA CGACTGTACC 
TATATAATCC GTGAGTTAGG GAATCGTAAT GAGTGCGATA AAGCTGTTGG CTTTTATGAG 
TTTGCGGTTA AACGAGAGAG GAGAAAGAAC GAGCAGGGGA AGTTAGCTAG TGCTATGATA 
AGTACTCTTG GTAGATATGG TAAAGTAACT ATTGCTAAGA GGATTTTCGA AACTGCTTTT 
GCTGGTGGAT ATGGAAACAC GGTTTACGCC TTCTCGGCTT TGATCAGCGC TTATGGGAGA 
AGTGGGCTAC ATGAGGAAGC CATTAGTGTC TTCAACTCCA TGAAAGAGTA TGGCTTGAGG 
CCGAATTTGG TTACCTACAA TGCTGTGATT GACGCTTGCG GTAAAGGCGG GATGGAGTTT 
AAGCAAGTAG CCAAGTTTTT CGATGAGATG CAGAGGAACG GTGTGCAGCC AGATAGAATA 
ACTTTCAACT CGTTGCTTGC TGTTTGCAGT AGAGGAGGGT TGTGGGAAGC AGCAAGGAAT 
CTGTTTGATG AGATGACGAA TAGAAGGATT GAGCAAGATG TTTTTTCTTA TAATACGCTT 
TTGGATGCAA TTTGCAAAGG TGGGCAGATG GACCTTGCTT TCGAAATCCT TGCTCAGATG 
CCAGTTAAGA GAATCATGCC TAATGTTGTA AGTTATAGCA CCGTTATTGA TGGGTTTGCA 
AAAGCCGGAA GATTTGATGA AGCTCTTAAC TTGTTTGGTG AAATGAGATA TCTTGGTATT 
GCATTAGATA GAGTTTCTTA CAATACATTG CTTTCAATCT ACACTAAAGT TGGTAGATCT 
GAAGAAGCTT TGGATATTCT GAGAGAAATG GCATCTGTTG GGATTAAAAA GGATGTTGTG 
ACTTATAATG CTCTTCTTGG AGGATATGGG AAACAAGGGA AATATGATGA AGTGAAGAAA 
GTTTTCACTG AGATGAAGCG AGAGCATGTG TTGCCTAACT TACTGACTTA TTCAACTTTG 
ATCGATGGGT ACTCGAAGGG GGGTTTGTAT AAAGAGGCAA TGGAGATATT TAGGGAGTTT 
AAGAGCGCCG GATTAAGAGC TGATGTTGTT TTGTACAGTG CATTGATTGA TGCATTGTGC 
AAGAATGGGT TGGTGGGTTC TGCTGTTTCT TTGATCGATG AGATGACAAA GGAAGGAATT 
AGTCCTAATG TTGTAACTTA TAATTCCATA ATCGATGCCT TTGGTCGTTC TGCAACTATG 
GACAGATCAG CCGATTATAG CAATGGTGGA TCTTTGCCTT TCTCTTCTTC TGCTCTGAGT 
GCATTGACTG AAACAGAGGG TAATCGGGTA ATACAGTTAT TTGGGCAGTT AACAACTGAG 
AGTAACAACC GAACGACGAA AGATTGTGAG GAGGGTATGC AGGAGCTCTC CTGTATATTG 
GAAGTTTTCC GCAAAATGCA TCAGCTGGAA ATTAAACCAA ATGTTGTAAC CTTTTCAGCG 
ATTCTGAATG CTTGCAGTCG GTGTAACTCA TTTGAAGATG CATCAATGCT ACTAGAGGAG 
CTCAGGCTGT TTGATAATAA GGTATATGGT GTTGTTCATG GACTTCTCAT GGGCCAGAGA 
GAGAATGTAT GGCTCCAAGC TCAGAGCTTA TTTGACAAAG TAAATGAAAT GGATGGTTCG 
ACTGCTTCTG CCTTCTACAA TGCCCTAACT GACATGCTTT GGCACTTTGG CCAGAAACGA 
GGTGCAGAAC TGGTGGCACT TGAGGGAAGA TCTAGGCAAG TTTGGGAAAA CGTGTGGTCT 
GATTCTTGCT TGGACTTACA CCTCATGTCT TCTGGAGCTG CACGTGCAAT GGTCCATGCG 
TGGCTACTAA ACATACGCTC CATTGTCTAT GAAGGTCATG AGTTACCTAA AGTCTTGAGT 
ATATTGACTG GCTGGGGGAA ACACAGCAAA GTGGTTGGAG ACGGAGCACT AAGACGAGCG 
GTTGAAGTTC TTTTGAGGGG AATGGATGCA CCATTCCACC TGTCAAAATG CAACATGGGA 
CGGTTCACAT CAAGCGGTTC GGTGGTAGCA ACTTGGCTGC GTGAATCAGC CACACTGAAA 
CTCTTAATCC TCCATGACCA CATAACAACC GCCACAGCTA CTACTACAAC AATGAAATCA 
ACAGACCAAC AACAACGGAA ACAGACGTCG TTTGCTTTAC AGCCTCTTCT TTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUN1 ( NM_001336330.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324971.1 Arabidopsis thaliana s uncoupled 1 (GUN1), mRNA.

Price & Availability↑

CloneID	OAb36197
Clone ID Related Accession (Same CDS sequence)	NM_001336330.1
Accession Version	NM_001336330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	s uncoupled 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGCGTCAA CGCCGCCTCA CTGGGTCACC ACCACCAACA ACCACCGTCC ATGGCTTCCT 
CAGCGTCCTC GTCCCGGTCG CTCCGTTACC TCAGCTCCTC CCTCTTCTTC AGCCTCTGTT 
TCTTCAGCTC ATCTTTCACA GACTACTCCA AATTTCTCTC CTTTGCAAAC CCCCAAATCA 
GATTTCTCCG GTCGTCAATC TACCCGATTC GTCTCCCCCG CCACCAATAA CCACCGTCAG 
ACCAGGCAAA ACCCAAACTA CAACCACCGT CCGTATGGTG CCTCTTCTTC TCCTCGTGGC 
TCAGCTCCTC CGCCTTCATC TGTAGCTACT GTAGCTCCAG CTCAACTATC ACAACCTCCT 
AACTTTTCGC CTCTCCAAAC CCCAAAATCC GATCTTTCAT CGGATTTTTC AGGTAGACGA 
TCGACTAGAT TCGTCTCCAA GATGCATTTC GGGCGGCAGA AGACGACAAT GGCTACGAGA 
CATAGCTCTG CTGCTGAAGA TGCACTTCAA AACGCTATTG ATTTCTCTGG AGACGATGAG 
ATGTTTCATA GTCTAATGTT GAGCTTTGAG TCAAAGCTGT GTGGCTCTGA CGACTGTACC 
TATATAATCC GTGAGTTAGG GAATCGTAAT GAGTGCGATA AAGCTGTTGG CTTTTATGAG 
TTTGCGGTTA AACGAGAGAG GAGAAAGAAC GAGCAGGGGA AGTTAGCTAG TGCTATGATA 
AGTACTCTTG GTAGATATGG TAAAGTAACT ATTGCTAAGA GGATTTTCGA AACTGCTTTT 
GCTGGTGGAT ATGGAAACAC GGTTTACGCC TTCTCGGCTT TGATCAGCGC TTATGGGAGA 
AGTGGGCTAC ATGAGGAAGC CATTAGTGTC TTCAACTCCA TGAAAGAGTA TGGCTTGAGG 
CCGAATTTGG TTACCTACAA TGCTGTGATT GACGCTTGCG GTAAAGGCGG GATGGAGTTT 
AAGCAAGTAG CCAAGTTTTT CGATGAGATG CAGAGGAACG GTGTGCAGCC AGATAGAATA 
ACTTTCAACT CGTTGCTTGC TGTTTGCAGT AGAGGAGGGT TGTGGGAAGC AGCAAGGAAT 
CTGTTTGATG AGATGACGAA TAGAAGGATT GAGCAAGATG TTTTTTCTTA TAATACGCTT 
TTGGATGCAA TTTGCAAAGG TGGGCAGATG GACCTTGCTT TCGAAATCCT TGCTCAGATG 
CCAGTTAAGA GAATCATGCC TAATGTTGTA AGTTATAGCA CCGTTATTGA TGGGTTTGCA 
AAAGCCGGAA GATTTGATGA AGCTCTTAAC TTGTTTGGTG AAATGAGATA TCTTGGTATT 
GCATTAGATA GAGTTTCTTA CAATACATTG CTTTCAATCT ACACTAAAGT TGGTAGATCT 
GAAGAAGCTT TGGATATTCT GAGAGAAATG GCATCTGTTG GGATTAAAAA GGATGTTGTG 
ACTTATAATG CTCTTCTTGG AGGATATGGG AAACAAGGGA AATATGATGA AGTGAAGAAA 
GTTTTCACTG AGATGAAGCG AGAGCATGTG TTGCCTAACT TACTGACTTA TTCAACTTTG 
ATCGATGGGT ACTCGAAGGG GGGTTTGTAT AAAGAGGCAA TGGAGATATT TAGGGAGTTT 
AAGAGCGCCG GATTAAGAGC TGATGTTGTT TTGTACAGTG CATTGATTGA TGCATTGTGC 
AAGAATGGGT TGGTGGGTTC TGCTGTTTCT TTGATCGATG AGATGACAAA GGAAGGAATT 
AGTCCTAATG TTGTAACTTA TAATTCCATA ATCGATGCCT TTGGTCGTTC TGCAACTATG 
GACAGATCAG CCGATTATAG CAATGGTGGA TCTTTGCCTT TCTCTTCTTC TGCTCTGAGT 
GCATTGACTG AAACAGAGGG TAATCGGGTA ATACAGTTAT TTGGGCAGTT AACAACTGAG 
AGTAACAACC GAACGACGAA AGATTGTGAG GAGGGTATGC AGGAGCTCTC CTGTATATTG 
GAAGTTTTCC GCAAAATGCA TCAGCTGGAA ATTAAACCAA ATGTTGTAAC CTTTTCAGCG 
ATTCTGAATG CTTGCAGTCG GTGTAACTCA TTTGAAGATG CATCAATGCT ACTAGAGGAG 
CTCAGGCTGT TTGATAATAA GGTATATGGT GTTGTTCATG GACTTCTCAT GGGCCAGAGA 
GAGAATGTAT GGCTCCAAGC TCAGAGCTTA TTTGACAAAG TAAATGAAAT GGATGGTTCG 
ACTGCTTCTG CCTTCTACAA TGCCCTAACT GACATGCTTT GGCACTTTGG CCAGAAACGA 
GGTGCAGAAC TGGTGGCACT TGAGGGAAGA TCTAGGCAAG TTTGGGAAAA CGTGTGGTCT 
GATTCTTGCT TGGACTTACA CCTCATGTCT TCTGGAGCTG CACGTGCAAT GGTCCATGCG 
TGGCTACTAA ACATACGCTC CATTGTCTAT GAAGGTCATG AGTTACCTAA AGTCTTGAGG 
TTGAAGTTTT TGGTCATACA CTATGATTAT AGTGTCTCTG TAGATACTTT GCTTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324971.1
CDS	229..2748
Translation	MASTPPHWVTTTNNHRPWLPQRPRPGRSVTSAPPSSSASVSSAHLSQTTPNFSPLQTPKSDFSGRQSTRFVSPATNNHRQTRQNPNYNHRPYGASSSPRGSAPPPSSVATVAPAQLSQPPNFSPLQTPKSDLSSDFSGRRSTRFVSKMHFGRQKTTMATRHSSAAEDALQNAIDFSGDDEMFHSLMLSFESKLCGSDDCTYIIRELGNRNECDKAVGFYEFAVKRERRKNEQGKLASAMISTLGRYGKVTIAKRIFETAFAGGYGNTVYAFSALISAYGRSGLHEEAISVFNSMKEYGLRPNLVTYNAVIDACGKGGMEFKQVAKFFDEMQRNGVQPDRITFNSLLAVCSRGGLWEAARNLFDEMTNRRIEQDVFSYNTLLDAICKGGQMDLAFEILAQMPVKRIMPNVVSYSTVIDGFAKAGRFDEALNLFGEMRYLGIALDRVSYNTLLSIYTKVGRSEEALDILREMASVGIKKDVVTYNALLGGYGKQGKYDEVKKVFTEMKREHVLPNLLTYSTLIDGYSKGGLYKEAMEIFREFKSAGLRADVVLYSALIDALCKNGLVGSAVSLIDEMTKEGISPNVVTYNSIIDAFGRSATMDRSADYSNGGSLPFSSSALSALTETEGNRVIQLFGQLTTESNNRTTKDCEEGMQELSCILEVFRKMHQLEIKPNVVTFSAILNACSRCNSFEDASMLLEELRLFDNKVYGVVHGLLMGQRENVWLQAQSLFDKVNEMDGSTASAFYNALTDMLWHFGQKRGAELVALEGRSRQVWENVWSDSCLDLHLMSSGAARAMVHAWLLNIRSIVYEGHELPKVLRLKFLVIHYDYSVSVDTLLM

Target ORF information:

RefSeq Version	NM_001336330.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana s uncoupled 1 (GUN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336330.1

ORF Insert Sequence:

ATGGCGTCAA CGCCGCCTCA CTGGGTCACC ACCACCAACA ACCACCGTCC ATGGCTTCCT 
CAGCGTCCTC GTCCCGGTCG CTCCGTTACC TCAGCTCCTC CCTCTTCTTC AGCCTCTGTT 
TCTTCAGCTC ATCTTTCACA GACTACTCCA AATTTCTCTC CTTTGCAAAC CCCCAAATCA 
GATTTCTCCG GTCGTCAATC TACCCGATTC GTCTCCCCCG CCACCAATAA CCACCGTCAG 
ACCAGGCAAA ACCCAAACTA CAACCACCGT CCGTATGGTG CCTCTTCTTC TCCTCGTGGC 
TCAGCTCCTC CGCCTTCATC TGTAGCTACT GTAGCTCCAG CTCAACTATC ACAACCTCCT 
AACTTTTCGC CTCTCCAAAC CCCAAAATCC GATCTTTCAT CGGATTTTTC AGGTAGACGA 
TCGACTAGAT TCGTCTCCAA GATGCATTTC GGGCGGCAGA AGACGACAAT GGCTACGAGA 
CATAGCTCTG CTGCTGAAGA TGCACTTCAA AACGCTATTG ATTTCTCTGG AGACGATGAG 
ATGTTTCATA GTCTAATGTT GAGCTTTGAG TCAAAGCTGT GTGGCTCTGA CGACTGTACC 
TATATAATCC GTGAGTTAGG GAATCGTAAT GAGTGCGATA AAGCTGTTGG CTTTTATGAG 
TTTGCGGTTA AACGAGAGAG GAGAAAGAAC GAGCAGGGGA AGTTAGCTAG TGCTATGATA 
AGTACTCTTG GTAGATATGG TAAAGTAACT ATTGCTAAGA GGATTTTCGA AACTGCTTTT 
GCTGGTGGAT ATGGAAACAC GGTTTACGCC TTCTCGGCTT TGATCAGCGC TTATGGGAGA 
AGTGGGCTAC ATGAGGAAGC CATTAGTGTC TTCAACTCCA TGAAAGAGTA TGGCTTGAGG 
CCGAATTTGG TTACCTACAA TGCTGTGATT GACGCTTGCG GTAAAGGCGG GATGGAGTTT 
AAGCAAGTAG CCAAGTTTTT CGATGAGATG CAGAGGAACG GTGTGCAGCC AGATAGAATA 
ACTTTCAACT CGTTGCTTGC TGTTTGCAGT AGAGGAGGGT TGTGGGAAGC AGCAAGGAAT 
CTGTTTGATG AGATGACGAA TAGAAGGATT GAGCAAGATG TTTTTTCTTA TAATACGCTT 
TTGGATGCAA TTTGCAAAGG TGGGCAGATG GACCTTGCTT TCGAAATCCT TGCTCAGATG 
CCAGTTAAGA GAATCATGCC TAATGTTGTA AGTTATAGCA CCGTTATTGA TGGGTTTGCA 
AAAGCCGGAA GATTTGATGA AGCTCTTAAC TTGTTTGGTG AAATGAGATA TCTTGGTATT 
GCATTAGATA GAGTTTCTTA CAATACATTG CTTTCAATCT ACACTAAAGT TGGTAGATCT 
GAAGAAGCTT TGGATATTCT GAGAGAAATG GCATCTGTTG GGATTAAAAA GGATGTTGTG 
ACTTATAATG CTCTTCTTGG AGGATATGGG AAACAAGGGA AATATGATGA AGTGAAGAAA 
GTTTTCACTG AGATGAAGCG AGAGCATGTG TTGCCTAACT TACTGACTTA TTCAACTTTG 
ATCGATGGGT ACTCGAAGGG GGGTTTGTAT AAAGAGGCAA TGGAGATATT TAGGGAGTTT 
AAGAGCGCCG GATTAAGAGC TGATGTTGTT TTGTACAGTG CATTGATTGA TGCATTGTGC 
AAGAATGGGT TGGTGGGTTC TGCTGTTTCT TTGATCGATG AGATGACAAA GGAAGGAATT 
AGTCCTAATG TTGTAACTTA TAATTCCATA ATCGATGCCT TTGGTCGTTC TGCAACTATG 
GACAGATCAG CCGATTATAG CAATGGTGGA TCTTTGCCTT TCTCTTCTTC TGCTCTGAGT 
GCATTGACTG AAACAGAGGG TAATCGGGTA ATACAGTTAT TTGGGCAGTT AACAACTGAG 
AGTAACAACC GAACGACGAA AGATTGTGAG GAGGGTATGC AGGAGCTCTC CTGTATATTG 
GAAGTTTTCC GCAAAATGCA TCAGCTGGAA ATTAAACCAA ATGTTGTAAC CTTTTCAGCG 
ATTCTGAATG CTTGCAGTCG GTGTAACTCA TTTGAAGATG CATCAATGCT ACTAGAGGAG 
CTCAGGCTGT TTGATAATAA GGTATATGGT GTTGTTCATG GACTTCTCAT GGGCCAGAGA 
GAGAATGTAT GGCTCCAAGC TCAGAGCTTA TTTGACAAAG TAAATGAAAT GGATGGTTCG 
ACTGCTTCTG CCTTCTACAA TGCCCTAACT GACATGCTTT GGCACTTTGG CCAGAAACGA 
GGTGCAGAAC TGGTGGCACT TGAGGGAAGA TCTAGGCAAG TTTGGGAAAA CGTGTGGTCT 
GATTCTTGCT TGGACTTACA CCTCATGTCT TCTGGAGCTG CACGTGCAAT GGTCCATGCG 
TGGCTACTAA ACATACGCTC CATTGTCTAT GAAGGTCATG AGTTACCTAA AGTCTTGAGG 
TTGAAGTTTT TGGTCATACA CTATGATTAT AGTGTCTCTG TAGATACTTT GCTTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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GUN1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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