AT2G25730 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT2G25730
Entrez Gene ID	817114
Full Name	zinc finger FYVE domain protein
Synonyms	F3N11.1,F3N11_1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001336018.1	NP_001324902.1	zinc finger FYVE domain protein
NM_001202673.2	NP_001189602.1	zinc finger FYVE domain protein
NM_001336017.1	NP_001324903.1	zinc finger FYVE domain protein
NM_128132.1	NP_565607.1	zinc finger FYVE domain protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT2G25730	NP_001189602.1
Oryza sativa (rice)	Os01g0593000	NP_001043457.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT2G25730 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT2G25730 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb00337	NM_128132.1 Latest version!	Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAb05592	NM_001202673.2 Latest version!	Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAb05592	NM_001336017.1 Latest version!	Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAb36030	NM_001336018.1 Latest version!	Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT2G25730 ( NM_128132.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_565607.1 Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), partial mRNA.

Price & Availability↑

CloneID	OAb00337
Clone ID Related Accession (Same CDS sequence)	NM_128132.1
Accession Version	NM_128132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	zinc finger FYVE domain protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). COMPLETENESS: incomplete on the 5' end.

ATGGATAAAG AGACGGAGAT TCTCTCCCGT CTCGCGGCGA ACCACCTTCA TCTGGCTCAA 
TTCGAGCCAT TGAAGGCTAC GTTACTCGCT CTCAGGGTTC GTAACCCTGA CCTCGCACTC 
ACCATTCTCC AAACCATCGT CTCCAACGCT GGAAGATTCG ATAATGTCCT CTGGTCACGC 
TCTTGTCCTT CCCCGTCTCT TCTCTCGTTC CTCTCCACGA TTGAGCTTCT GAGATTCGAA 
AATCCTACTT CTCCTTGGGG ATTTGATTCA GAAACTCTAA GTTTGCGTGC CGATTTCTTG 
TTGATGGTTC AGGTTTTGAT CGATAGAGTT ACAGAGAGGA TTAAGGAAGA TGAGGAGAGT 
GAGGATGAAA ATTCTGGATT AGGGAATTGT TTAAGGGTGT TGCAAGGTGT TTTGGAGTTA 
GGTGTTGAGA GGTTGAAGTT TGTTGTTGAT ACTAGTAGTA GTGAAGGAAG TAATAAGATT 
GAGGAAGATG CAGTTGTGTC TTTGAGGAGT ATAGTATTGG ATTACTCTGA TGTTTTCGAT 
GCTTTATGTT GTAATATTCA GAGGCAACTT GCGGGTTGCG AGAGTTACGG TACATGTTTG 
GTTGAGGAAG TTCAGGGAGA AGAACAGAGA AAGGAGATGA ATGAGGCCAC ATGTATTGGT 
TCTCCGGAGC TGGATAACAT CAATGTGTTT GCTTTGATAC AGAGGAATGT TCAGTTAGCA 
CAGTTGGATG CTATGAAAAC AAAGTTGGAT GAAGGTGATG AGCGCGGGGC AGCTGATCGC 
ATTCGTTATC TTCACCTTGA TTATGGAGTA GAGAAAGAGA ACTATCATGC TGTTCTAAAA 
GCTCTCCTTT CAAGAGTTAT GGAGAAAAAG GATGAATATG GTGATTCCTG GCACATGGTG 
CGCCAGAACT TGCTGTTTAT GTATAAAGAA GCTCTCTCAT CGAATTGTGG AGATCTTGTT 
CAGATGATCC AGGGTATTCA AGATGATATG CTCCTCCCAC ATAGCCAACT ACATTTATCT 
CTCGACAATG AACAAATTCC ACTCCCTCTT GAATGTTTCC GGCGATATCT TGTAGACTTG 
AAAACTGAGA GAAATATAGA GGACAAAAGT TCTCCTATGA GCAGGGCAAT TAATTCTTGT 
CTCAGAGATA TGTATCATTA TGCTCGTATT TCTGGATCAC ATGTTCTTGA GTGTGTGATG 
TGTGCTGCTT TGTCTTCTGT AAAGAAAGAG AAGCTTCAGG AGGCTAATGA TGTTCTTACT 
TTGTTTCCCC GACTTCGCCC TTTAGTAGCC TCCATGGGTT GGGATCTATT GCCGGGCAAA 
ACTGCAACCC GTAGAAAATT GATGCGGCTA CTTTGGACTA GTGACTCGCA AGCACTTCGG 
CTAGAAGAAT CTTCTCTTTA TGGAAACCAG ACAGATGAAC TGGAACTTGC ATCTTTCGCT 
GCTTGTGTCA ATTCTGGTAA ATCATGGACT CCAAAGGCAT CTTTCTTGAT GCATGGTAAT 
GTGTCATCCG CGCATGATGA TGCGGAGGTG GATCCTTTTG TTGAAAATCT TGTATTGGAA 
AGGCTTTCAG CGCAAAGTCC ACTTCGGGTA TTGTTTGACG TTGTTCCGGG CATAAAATTC 
CAAGATGCTA TTTCACTGAT TAGTATGCAA CCTATTGCTT CAACTGCAGA AGCCTGGAAG 
AGGATAGAAG ATATTGAACT GATGCATATG CGTTATGCTC TGGAGGCAAT CGTTTTAGCA 
CTAGGTGCAA TGGAAGAGGC TATGAAGGAT GAGACAGATG CTAGTCATCG AGTAGTATTT 
TACCATTTAA AAGACCTCAC TAACCATTTG GAGGCCATTA AAAATGTTCC ACGCAAGATA 
ATGATGGTGA ACATAGTTAT TTCACTCTTA CATATTGATG ATATCCGTCT CAGTTCTACG 
CAAAGTGCCT CCTCGGCATG TTTTTCTGAA AAAAGTAACA CACCTGGTTT GGATCCTGGC 
GATCTTGGTA CAGAAGGGGA AAAGGAAATT GTTATTTCTT TCACAAAACA GCTACTCGAT 
GTTTTACGCC GCAATCTTCC ATCACATCCA ATTGAACAAG AGTGTCAGCT GGATGGTAAT 
TACAGTACTG ATGGAAGACA GGCTTTAGAA TGGAGAGTAT CCATGGCTAA GCGTTTCATT 
GAAGATTGTG AATGGCGATT ATCTGTTATG CAGCATCTTC TGCCACTTTC TGAACGCCAG 
TGGGGTTTAA AGGAGGTTTT GAGTATTCTA AGGGCAGCCC CTGAAAAACT GCTTAATCTC 
TGTATGCAAA GAGCTAAGTA TGACATTGGA GAAGAGGCAG TTAATCGGTT TGCGTTATCA 
GCAGAGGACA AAGCTACTCT TGAATTAGCT GAATGGGTTG ATAATGCGTT CAAAGGAACA 
CTGGTAGAAG ATGTAATGTC TCGTACTGCT GAAGGAGCAG CTGCCGTGCA AGATTTAGAT 
TTTCATTCTT TAGGTTCTCA ATTGAGTCCA TTGGCTATGG TTTTACTTTT TGCGCAGTCT 
CAAGTTATGT TATCGGAAAT TTACCCTGGA GGAGCTCCGA AGGTGGGGTT TACTTACTGG 
GATCAGGTCC ACGAAGTTGC AATAATTTCT GTATTGCGAA GGATCTTAAA GCGTCTGCAG 
GAATTCCTTG AACAGGATGA CCCTCAAATT CTTCAAGCCA GTTTTAGTGG AGATACCATA 
ATTTCATCTT GCACGGAATC TCATAGACAG GGACAAAAAG ATCGTGCTCT TGCAATGCTA 
CATCAAATGA TTGAGGATGC TCATAGGGGC AAGCGTCAGT TCCTGAGTGG TAAGCTTCAT 
AACTTAGCGA GAGCACTCGC TGATGAAAAA CCAGAAGTTG ACGTACTCAA AGGGGACGGA 
TCAGACATGG CCGTTGAGAA GGATGGAGTT CTTGGTCTTG GGCTAAAATA TACAAAGCAA 
AGTCCTGGTT CAGCAAATAG AGCCGTGGAT GGAAATCCTG TTTCACATGA AACAGAAGAC 
AAGGGAAAGA AGTCATTTGG CCCATTAAGC AACAAAACCT CTACTTATCT ATCTCAGTTT 
ATCCTCTATA CTGCTGCTAT TGGTGATATA GTAGATGGAA CTGACACAAC CCATGATTTC 
AACTTTTTCT CTCTTGTTTA TGAATGGCCT AAAGACCTAT TGACGCGTCT GGTTTTTGAT 
CGAAGTAGCA CAGATGCAGC TGCAAAAGTT GCTGAGGTTA TGTCTGCTGA TTTTGTTCAT 
GAAGTGATAT CAGCATGTGT TCCCCCAGTT TATCCCCCAC GTTCTGGTCA TGGGTGGGCT 
TGTATTCCCG TCATTCCAAC CACTCCATGT TCCCACTCAG AGGGTAAAGT GCTCTCTCCT 
TCAATAGAGG CTAAACCCAA CTGTTATGTC CGTTCCTCAG CAACACCTGG TGTCCCTCTG 
TATCCTCTTC AGTTGGATGT TATCAGGCAT TTGGTAAAAA TTTCACCAGT ACGAGCAGTT 
TTAGCTTGCG TCTTTGGTGG GAGCATATTG TACAATGGCA GTGATTCTAT CATATCTAGC 
TCCTTGAACG ATGAGTTTCC AAGTTCTCCT GATGCAGACA GATTGTTTTA TGAATTTTCT 
CTTGATCAGT CTGAGAGGTA TCCCACTTTA AACCGATGGA TACAGATGCA GACTAATCTG 
CATCGAGTTT CGGAATTTGT TGTGACACCG AAGCAAAAAC CTGATGACAC ACGGATTAAG 
CCTGATGAAA GAACTGGGAT CAAGAGACTT CTTGAACATG ATAGTGACTC AGAGTCAGAT 
ACAGAAGAAA CATTTTCTAA AAATAACATT CAACCAGCAT TGACAGACGG CAGTGCTCGT 
GATGGTGGAT CCTTTGAAAA TGGAGTTTGT AGAACTGATC CTACCGTTTT CCTTTCTTTT 
GATTGGGAGA ATGAAGTACC GTATGAGAAA GCTGTAAATA GACTAATTGA TGAAGGAAAA 
CTAATGGATG CTTTAGCACT TTCGGACCGC TTCTTGCGGA ATGGGGCTTC TGATTGGTTA 
CTTCAGCTCC TAATCAAAAG TAGAGAAGAA AATCCTTCAA CATCAGGACG ATCTCAGGGA 
TATGGAGGCC AGAGCAACAG TTGGCAGTAT TGCCTGCGGC TAAAGGACAA ACAGCTGGCA 
GCAACACTGG CTCTTAAGTG TTGCATAGGA GACAAGCTCT GCAGAAGTAC AGCCACATAC 
TTTCGGCAGA TGATCGCCAT AATAGCTGGC AAGAGGTTAT CTTTCTTCCT TTTGTTTGAG 
ATCATGTTTG GCTCTTGGTA TGCTCGATGT GTCACTCTCA AAAATCTAAA TGGGAAACAG 
GTAGAAGCCG AATGTAAAGA AGACCCTGAA GGCTTGGCTC TAAGATTGGC TGGGAAAGGA 
GCTGTTTCCG CTGCGCTAGA AGTGGCTGAA AGTGCAGGAT TATCAATAGA TCTTAGAAGA 
GAATTACAAG GACGGCAGCT TGTAAAGCTT CTAACCACTG ATCCACTCAA TGGTGGTGGT 
CCGGCGGAAG CATCTCGGTT TTTATCTTCA CTTCAAGACT CGGCTGATGC TCTACCAGTG 
GTTATGGGTG CAATGCAATT ATTACCTGAC CTTCGGTCGA AACAGCTCCT GGTCCATTTC 
TTTCTCAAGC GAAGAGATAG CAATCTGTCG GATTTGGAAG TCGCCCGGCT TAATTCTTGG 
GCTTTAGGTC TGAAAGTGTT AGCTGCATTA CCACTTCCTT GGCAACAGAG ATGCTCTTCG 
CTTCATGAGC ATCCCAATTT AATATTTGAA GCTCTGCTAA TGAGAAAACA ATTACAATAC 
GCCTCACTGA TACTCAAAGA GTTCCCAGCA TTAAGAGATA ACAATGTTAT CATGGCCTAT 
GCAGCAAAAG CTATTTCTGT GACAATTATC CCACCACCAA GAGAACCTCG AATAACTGTG 
TCTGCGTCAA GGTTAAGACA GAAATCAAGA GCAGGGCCAG CAGTAAAAGC ATCCTTCACT 
AGTAGCTTAA GCAATTTTCA GAGAGAGGCT CGAAGGGCAT TCTCATGGGC TCCACGTAAT 
GCTGAAAACC GGACGACGTC AAAGGATGTT TATCGCAAAA GAAAGAATTC CGGACTGGGG 
GCTTCTGAGA GAGCTGCATG GGAGGCGATG ACAGGTATTC AAGAGGACCA GGGATCATCT 
TATTCGGCAG ATGGGCAAGA TAGGCTACCT TCTGTTTCTA TTGCTGAAGA ATGGATGCTA 
ACTGGCGACA AAACCAAAGA TGAAGGTGTT CGTGCATCTC ACAAGTATGA AAGCACCCCC 
GATATTATTC TCTTTAAGGC TCTACTATCA CTTTGTTCAG ATGAGCTAGT ATCAGCAAGA 
AGTGCCATGG ACCTATGCAT CAGTCAAATG AAAAACGTAT TGAGCTCCAA ACAGTTGTCA 
GAGGGTGCGT CTGTTGAAAC AATTGGCCGA GCATATCATG CAACGGAGGC ATTTGTGCAG 
GGTTTGTCGT ATGCAAAATC ATTGCTGAGA AAGCTTCTAG GTACCACTGA ATCGACCAAT 
AACAATGGTG AAAGAAGTAG AGATGTTGAT GATATATCTT CTGATGCTGG CAGCTCCAGT 
GTTGGCAGTC AGTCAACGGA TGAACCATCG GATGTTCTCT CACTTACAGA AATCTGGTTG 
GGGCGCGCAG AGTTGCTGCA GAGCCTCTTA GGGTCTGGAA TTTCTACTTC TCTTGATGAC 
ATTGCTGATC AATTGTCATC CGAATGTCTA CGAGACAGAT TAATATCTGA TGAACGATAT 
AGTATGGCGG TGTATATGTG CAAGAAATGC AAGATTGATG TTTTCCCCGT GTGGAAAGCA 
TGGGGTCTTG CTTTGTTACG GATGGAGCGC TATGCTCAAG CTCGAGTCAA ATTCAAGCAA 
GCCTTTCAAT TAAAGGGGGA AGACATTCCT GATGTCATTC AGGAGATAAT AAATACAATA 
GAAGGAGGTC CGCCTGTGGA TGTGTCAATT GTACGTTCCA TGTATGACCA TTTGGCGAAA 
AGCGCACCTA CAATTTTGGA CGATTCTTTA TCAGCAGACT CATACCTAAA TGTTCTGCAT 
ATGCCATCCA CTTTCCCTCG TTCAGAGAGG TCGCGGAGAT CTCTGGAATC GGAAAAGAAT 
AGTTCTGTAC CTGGTTCAGA CTTTGAAGAT GGGCCCCGAA GCAACTTGGA TACTACACGC 
TATTCTGAGT GCACCAACTA CTTGCAGGAA CATGCTCGCC AAAACCTGCT TGGGTTTATG 
TTCCGCCATG GTCACTTTAA AGATGCATGC ATGTTATTCT TTCCGCAAAG TGGTCTTCCC 
CCTCCTTTGC AAACTTCATC TGTGGGCGCA GTAAGCACAT CTTCATCACC TCAACGAACT 
GATCCCTTGG CAACTGAATA TGGGACCATT GAAAGTTTGT GCGAGTTCTG TGTTGGTTAT 
GGAGCTATCT CATCCCTTGA GGAAGTAATT ACAGAAAGAC TTGAATCCGC AAAGAATCAA 
GATCAAGCCA TAAATCAGTA CATAGCTGGA GCTCTTACTC GTATCTGTGC TTTCTTTGAG 
ATCAACCGGC ATTTCAATTA CCTATACAAG TTTCTGGTAC TCAAGAAGGA TTATGTCACC 
TCTGGGTATT GTTGTATTCA GCTTTTTATG AATTCTACAA CTCAGGAGGA TGCTGTAAGG 
CATCTTGAGC ATGCAAAGAA ATACTGGTCT CTAACTATCC TCGGGGTACA GGCACACTTT 
GAAGAAGCAT TGACAGCGCG TCATAGAGGT TCAGACTCAA AAAAACTTGT TACAAAGGGT 
GTTAGAGGAA AAAGTGCCGC AGAGAAGCTG AGTGAAGAAA CTCTTGTTAA GTTGTCCTCA 
CGGGTGAAAA TGCAGATTGA TGTGGTGAAG TCCTTCAGTG ACTCTGAAGG AGCACCATGG 
AAGCATTCCT TGTTTGGAAA TCCAAATGAT TCAGAGACAT CCAGGAGAAG ATGTGAAATA 
GTGGAGACTC TTGTTGAGAA AAATTTCGAC TTAGCTTATT CTGTTATATA TGAATTCAAG 
CTCTCAGCTG TTGATATATA TGCTGGTGTT GCTACGTCAC TAGCTGATAG GAAGAAAGGC 
AGTCAGTTGA CAGAACTTTT CAAAAACATT AAGGGAACAA TCCAGGATGA TGACTGGGAT 
CAGGTCCTGG GTGCTGCCAT CAATATATAT GCCAACAAGC ACAAAGAGCG CCCTGACCGT 
CTCATCGACA TGTTAACAAG CAGCCATCGA AAGGTGCTGG CTTGTGTGGT ATGTGGCCGC 
CTGAAAAGCG CATTCCAGAT TGCATCTAAA AGCGGAAGCG TGGCTGATGT TCAATATGTA 
GCTCATCAAG CCTTACATGC CAATTCGCAC ACAGTACTCG ATATGTGCAA GCAATGGCTA 
GCTAAATACA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_565607.1
CDS	1..7395
Translation	MDKETEILSRLAANHLHLAQFEPLKATLLALRVRNPDLALTILQTIVSNAGRFDNVLWSRSCPSPSLLSFLSTIELLRFENPTSPWGFDSETLSLRADFLLMVQVLIDRVTERIKEDEESEDENSGLGNCLRVLQGVLELGVERLKFVVDTSSSEGSNKIEEDAVVSLRSIVLDYSDVFDALCCNIQRQLAGCESYGTCLVEEVQGEEQRKEMNEATCIGSPELDNINVFALIQRNVQLAQLDAMKTKLDEGDERGAADRIRYLHLDYGVEKENYHAVLKALLSRVMEKKDEYGDSWHMVRQNLLFMYKEALSSNCGDLVQMIQGIQDDMLLPHSQLHLSLDNEQIPLPLECFRRYLVDLKTERNIEDKSSPMSRAINSCLRDMYHYARISGSHVLECVMCAALSSVKKEKLQEANDVLTLFPRLRPLVASMGWDLLPGKTATRRKLMRLLWTSDSQALRLEESSLYGNQTDELELASFAACVNSGKSWTPKASFLMHGNVSSAHDDAEVDPFVENLVLERLSAQSPLRVLFDVVPGIKFQDAISLISMQPIASTAEAWKRIEDIELMHMRYALEAIVLALGAMEEAMKDETDASHRVVFYHLKDLTNHLEAIKNVPRKIMMVNIVISLLHIDDIRLSSTQSASSACFSEKSNTPGLDPGDLGTEGEKEIVISFTKQLLDVLRRNLPSHPIEQECQLDGNYSTDGRQALEWRVSMAKRFIEDCEWRLSVMQHLLPLSERQWGLKEVLSILRAAPEKLLNLCMQRAKYDIGEEAVNRFALSAEDKATLELAEWVDNAFKGTLVEDVMSRTAEGAAAVQDLDFHSLGSQLSPLAMVLLFAQSQVMLSEIYPGGAPKVGFTYWDQVHEVAIISVLRRILKRLQEFLEQDDPQILQASFSGDTIISSCTESHRQGQKDRALAMLHQMIEDAHRGKRQFLSGKLHNLARALADEKPEVDVLKGDGSDMAVEKDGVLGLGLKYTKQSPGSANRAVDGNPVSHETEDKGKKSFGPLSNKTSTYLSQFILYTAAIGDIVDGTDTTHDFNFFSLVYEWPKDLLTRLVFDRSSTDAAAKVAEVMSADFVHEVISACVPPVYPPRSGHGWACIPVIPTTPCSHSEGKVLSPSIEAKPNCYVRSSATPGVPLYPLQLDVIRHLVKISPVRAVLACVFGGSILYNGSDSIISSSLNDEFPSSPDADRLFYEFSLDQSERYPTLNRWIQMQTNLHRVSEFVVTPKQKPDDTRIKPDERTGIKRLLEHDSDSESDTEETFSKNNIQPALTDGSARDGGSFENGVCRTDPTVFLSFDWENEVPYEKAVNRLIDEGKLMDALALSDRFLRNGASDWLLQLLIKSREENPSTSGRSQGYGGQSNSWQYCLRLKDKQLAATLALKCCIGDKLCRSTATYFRQMIAIIAGKRLSFFLLFEIMFGSWYARCVTLKNLNGKQVEAECKEDPEGLALRLAGKGAVSAALEVAESAGLSIDLRRELQGRQLVKLLTTDPLNGGGPAEASRFLSSLQDSADALPVVMGAMQLLPDLRSKQLLVHFFLKRRDSNLSDLEVARLNSWALGLKVLAALPLPWQQRCSSLHEHPNLIFEALLMRKQLQYASLILKEFPALRDNNVIMAYAAKAISVTIIPPPREPRITVSASRLRQKSRAGPAVKASFTSSLSNFQREARRAFSWAPRNAENRTTSKDVYRKRKNSGLGASERAAWEAMTGIQEDQGSSYSADGQDRLPSVSIAEEWMLTGDKTKDEGVRASHKYESTPDIILFKALLSLCSDELVSARSAMDLCISQMKNVLSSKQLSEGASVETIGRAYHATEAFVQGLSYAKSLLRKLLGTTESTNNNGERSRDVDDISSDAGSSSVGSQSTDEPSDVLSLTEIWLGRAELLQSLLGSGISTSLDDIADQLSSECLRDRLISDERYSMAVYMCKKCKIDVFPVWKAWGLALLRMERYAQARVKFKQAFQLKGEDIPDVIQEIINTIEGGPPVDVSIVRSMYDHLAKSAPTILDDSLSADSYLNVLHMPSTFPRSERSRRSLESEKNSSVPGSDFEDGPRSNLDTTRYSECTNYLQEHARQNLLGFMFRHGHFKDACMLFFPQSGLPPPLQTSSVGAVSTSSSPQRTDPLATEYGTIESLCEFCVGYGAISSLEEVITERLESAKNQDQAINQYIAGALTRICAFFEINRHFNYLYKFLVLKKDYVTSGYCCIQLFMNSTTQEDAVRHLEHAKKYWSLTILGVQAHFEEALTARHRGSDSKKLVTKGVRGKSAAEKLSEETLVKLSSRVKMQIDVVKSFSDSEGAPWKHSLFGNPNDSETSRRRCEIVETLVEKNFDLAYSVIYEFKLSAVDIYAGVATSLADRKKGSQLTELFKNIKGTIQDDDWDQVLGAAINIYANKHKERPDRLIDMLTSSHRKVLACVVCGRLKSAFQIASKSGSVADVQYVAHQALHANSHTVLDMCKQWLAKYM

Target ORF information:

RefSeq Version	NM_128132.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_128132.1

ORF Insert Sequence:

ATGGATAAAG AGACGGAGAT TCTCTCCCGT CTCGCGGCGA ACCACCTTCA TCTGGCTCAA 
TTCGAGCCAT TGAAGGCTAC GTTACTCGCT CTCAGGGTTC GTAACCCTGA CCTCGCACTC 
ACCATTCTCC AAACCATCGT CTCCAACGCT GGAAGATTCG ATAATGTCCT CTGGTCACGC 
TCTTGTCCTT CCCCGTCTCT TCTCTCGTTC CTCTCCACGA TTGAGCTTCT GAGATTCGAA 
AATCCTACTT CTCCTTGGGG ATTTGATTCA GAAACTCTAA GTTTGCGTGC CGATTTCTTG 
TTGATGGTTC AGGTTTTGAT CGATAGAGTT ACAGAGAGGA TTAAGGAAGA TGAGGAGAGT 
GAGGATGAAA ATTCTGGATT AGGGAATTGT TTAAGGGTGT TGCAAGGTGT TTTGGAGTTA 
GGTGTTGAGA GGTTGAAGTT TGTTGTTGAT ACTAGTAGTA GTGAAGGAAG TAATAAGATT 
GAGGAAGATG CAGTTGTGTC TTTGAGGAGT ATAGTATTGG ATTACTCTGA TGTTTTCGAT 
GCTTTATGTT GTAATATTCA GAGGCAACTT GCGGGTTGCG AGAGTTACGG TACATGTTTG 
GTTGAGGAAG TTCAGGGAGA AGAACAGAGA AAGGAGATGA ATGAGGCCAC ATGTATTGGT 
TCTCCGGAGC TGGATAACAT CAATGTGTTT GCTTTGATAC AGAGGAATGT TCAGTTAGCA 
CAGTTGGATG CTATGAAAAC AAAGTTGGAT GAAGGTGATG AGCGCGGGGC AGCTGATCGC 
ATTCGTTATC TTCACCTTGA TTATGGAGTA GAGAAAGAGA ACTATCATGC TGTTCTAAAA 
GCTCTCCTTT CAAGAGTTAT GGAGAAAAAG GATGAATATG GTGATTCCTG GCACATGGTG 
CGCCAGAACT TGCTGTTTAT GTATAAAGAA GCTCTCTCAT CGAATTGTGG AGATCTTGTT 
CAGATGATCC AGGGTATTCA AGATGATATG CTCCTCCCAC ATAGCCAACT ACATTTATCT 
CTCGACAATG AACAAATTCC ACTCCCTCTT GAATGTTTCC GGCGATATCT TGTAGACTTG 
AAAACTGAGA GAAATATAGA GGACAAAAGT TCTCCTATGA GCAGGGCAAT TAATTCTTGT 
CTCAGAGATA TGTATCATTA TGCTCGTATT TCTGGATCAC ATGTTCTTGA GTGTGTGATG 
TGTGCTGCTT TGTCTTCTGT AAAGAAAGAG AAGCTTCAGG AGGCTAATGA TGTTCTTACT 
TTGTTTCCCC GACTTCGCCC TTTAGTAGCC TCCATGGGTT GGGATCTATT GCCGGGCAAA 
ACTGCAACCC GTAGAAAATT GATGCGGCTA CTTTGGACTA GTGACTCGCA AGCACTTCGG 
CTAGAAGAAT CTTCTCTTTA TGGAAACCAG ACAGATGAAC TGGAACTTGC ATCTTTCGCT 
GCTTGTGTCA ATTCTGGTAA ATCATGGACT CCAAAGGCAT CTTTCTTGAT GCATGGTAAT 
GTGTCATCCG CGCATGATGA TGCGGAGGTG GATCCTTTTG TTGAAAATCT TGTATTGGAA 
AGGCTTTCAG CGCAAAGTCC ACTTCGGGTA TTGTTTGACG TTGTTCCGGG CATAAAATTC 
CAAGATGCTA TTTCACTGAT TAGTATGCAA CCTATTGCTT CAACTGCAGA AGCCTGGAAG 
AGGATAGAAG ATATTGAACT GATGCATATG CGTTATGCTC TGGAGGCAAT CGTTTTAGCA 
CTAGGTGCAA TGGAAGAGGC TATGAAGGAT GAGACAGATG CTAGTCATCG AGTAGTATTT 
TACCATTTAA AAGACCTCAC TAACCATTTG GAGGCCATTA AAAATGTTCC ACGCAAGATA 
ATGATGGTGA ACATAGTTAT TTCACTCTTA CATATTGATG ATATCCGTCT CAGTTCTACG 
CAAAGTGCCT CCTCGGCATG TTTTTCTGAA AAAAGTAACA CACCTGGTTT GGATCCTGGC 
GATCTTGGTA CAGAAGGGGA AAAGGAAATT GTTATTTCTT TCACAAAACA GCTACTCGAT 
GTTTTACGCC GCAATCTTCC ATCACATCCA ATTGAACAAG AGTGTCAGCT GGATGGTAAT 
TACAGTACTG ATGGAAGACA GGCTTTAGAA TGGAGAGTAT CCATGGCTAA GCGTTTCATT 
GAAGATTGTG AATGGCGATT ATCTGTTATG CAGCATCTTC TGCCACTTTC TGAACGCCAG 
TGGGGTTTAA AGGAGGTTTT GAGTATTCTA AGGGCAGCCC CTGAAAAACT GCTTAATCTC 
TGTATGCAAA GAGCTAAGTA TGACATTGGA GAAGAGGCAG TTAATCGGTT TGCGTTATCA 
GCAGAGGACA AAGCTACTCT TGAATTAGCT GAATGGGTTG ATAATGCGTT CAAAGGAACA 
CTGGTAGAAG ATGTAATGTC TCGTACTGCT GAAGGAGCAG CTGCCGTGCA AGATTTAGAT 
TTTCATTCTT TAGGTTCTCA ATTGAGTCCA TTGGCTATGG TTTTACTTTT TGCGCAGTCT 
CAAGTTATGT TATCGGAAAT TTACCCTGGA GGAGCTCCGA AGGTGGGGTT TACTTACTGG 
GATCAGGTCC ACGAAGTTGC AATAATTTCT GTATTGCGAA GGATCTTAAA GCGTCTGCAG 
GAATTCCTTG AACAGGATGA CCCTCAAATT CTTCAAGCCA GTTTTAGTGG AGATACCATA 
ATTTCATCTT GCACGGAATC TCATAGACAG GGACAAAAAG ATCGTGCTCT TGCAATGCTA 
CATCAAATGA TTGAGGATGC TCATAGGGGC AAGCGTCAGT TCCTGAGTGG TAAGCTTCAT 
AACTTAGCGA GAGCACTCGC TGATGAAAAA CCAGAAGTTG ACGTACTCAA AGGGGACGGA 
TCAGACATGG CCGTTGAGAA GGATGGAGTT CTTGGTCTTG GGCTAAAATA TACAAAGCAA 
AGTCCTGGTT CAGCAAATAG AGCCGTGGAT GGAAATCCTG TTTCACATGA AACAGAAGAC 
AAGGGAAAGA AGTCATTTGG CCCATTAAGC AACAAAACCT CTACTTATCT ATCTCAGTTT 
ATCCTCTATA CTGCTGCTAT TGGTGATATA GTAGATGGAA CTGACACAAC CCATGATTTC 
AACTTTTTCT CTCTTGTTTA TGAATGGCCT AAAGACCTAT TGACGCGTCT GGTTTTTGAT 
CGAAGTAGCA CAGATGCAGC TGCAAAAGTT GCTGAGGTTA TGTCTGCTGA TTTTGTTCAT 
GAAGTGATAT CAGCATGTGT TCCCCCAGTT TATCCCCCAC GTTCTGGTCA TGGGTGGGCT 
TGTATTCCCG TCATTCCAAC CACTCCATGT TCCCACTCAG AGGGTAAAGT GCTCTCTCCT 
TCAATAGAGG CTAAACCCAA CTGTTATGTC CGTTCCTCAG CAACACCTGG TGTCCCTCTG 
TATCCTCTTC AGTTGGATGT TATCAGGCAT TTGGTAAAAA TTTCACCAGT ACGAGCAGTT 
TTAGCTTGCG TCTTTGGTGG GAGCATATTG TACAATGGCA GTGATTCTAT CATATCTAGC 
TCCTTGAACG ATGAGTTTCC AAGTTCTCCT GATGCAGACA GATTGTTTTA TGAATTTTCT 
CTTGATCAGT CTGAGAGGTA TCCCACTTTA AACCGATGGA TACAGATGCA GACTAATCTG 
CATCGAGTTT CGGAATTTGT TGTGACACCG AAGCAAAAAC CTGATGACAC ACGGATTAAG 
CCTGATGAAA GAACTGGGAT CAAGAGACTT CTTGAACATG ATAGTGACTC AGAGTCAGAT 
ACAGAAGAAA CATTTTCTAA AAATAACATT CAACCAGCAT TGACAGACGG CAGTGCTCGT 
GATGGTGGAT CCTTTGAAAA TGGAGTTTGT AGAACTGATC CTACCGTTTT CCTTTCTTTT 
GATTGGGAGA ATGAAGTACC GTATGAGAAA GCTGTAAATA GACTAATTGA TGAAGGAAAA 
CTAATGGATG CTTTAGCACT TTCGGACCGC TTCTTGCGGA ATGGGGCTTC TGATTGGTTA 
CTTCAGCTCC TAATCAAAAG TAGAGAAGAA AATCCTTCAA CATCAGGACG ATCTCAGGGA 
TATGGAGGCC AGAGCAACAG TTGGCAGTAT TGCCTGCGGC TAAAGGACAA ACAGCTGGCA 
GCAACACTGG CTCTTAAGTG TTGCATAGGA GACAAGCTCT GCAGAAGTAC AGCCACATAC 
TTTCGGCAGA TGATCGCCAT AATAGCTGGC AAGAGGTTAT CTTTCTTCCT TTTGTTTGAG 
ATCATGTTTG GCTCTTGGTA TGCTCGATGT GTCACTCTCA AAAATCTAAA TGGGAAACAG 
GTAGAAGCCG AATGTAAAGA AGACCCTGAA GGCTTGGCTC TAAGATTGGC TGGGAAAGGA 
GCTGTTTCCG CTGCGCTAGA AGTGGCTGAA AGTGCAGGAT TATCAATAGA TCTTAGAAGA 
GAATTACAAG GACGGCAGCT TGTAAAGCTT CTAACCACTG ATCCACTCAA TGGTGGTGGT 
CCGGCGGAAG CATCTCGGTT TTTATCTTCA CTTCAAGACT CGGCTGATGC TCTACCAGTG 
GTTATGGGTG CAATGCAATT ATTACCTGAC CTTCGGTCGA AACAGCTCCT GGTCCATTTC 
TTTCTCAAGC GAAGAGATAG CAATCTGTCG GATTTGGAAG TCGCCCGGCT TAATTCTTGG 
GCTTTAGGTC TGAAAGTGTT AGCTGCATTA CCACTTCCTT GGCAACAGAG ATGCTCTTCG 
CTTCATGAGC ATCCCAATTT AATATTTGAA GCTCTGCTAA TGAGAAAACA ATTACAATAC 
GCCTCACTGA TACTCAAAGA GTTCCCAGCA TTAAGAGATA ACAATGTTAT CATGGCCTAT 
GCAGCAAAAG CTATTTCTGT GACAATTATC CCACCACCAA GAGAACCTCG AATAACTGTG 
TCTGCGTCAA GGTTAAGACA GAAATCAAGA GCAGGGCCAG CAGTAAAAGC ATCCTTCACT 
AGTAGCTTAA GCAATTTTCA GAGAGAGGCT CGAAGGGCAT TCTCATGGGC TCCACGTAAT 
GCTGAAAACC GGACGACGTC AAAGGATGTT TATCGCAAAA GAAAGAATTC CGGACTGGGG 
GCTTCTGAGA GAGCTGCATG GGAGGCGATG ACAGGTATTC AAGAGGACCA GGGATCATCT 
TATTCGGCAG ATGGGCAAGA TAGGCTACCT TCTGTTTCTA TTGCTGAAGA ATGGATGCTA 
ACTGGCGACA AAACCAAAGA TGAAGGTGTT CGTGCATCTC ACAAGTATGA AAGCACCCCC 
GATATTATTC TCTTTAAGGC TCTACTATCA CTTTGTTCAG ATGAGCTAGT ATCAGCAAGA 
AGTGCCATGG ACCTATGCAT CAGTCAAATG AAAAACGTAT TGAGCTCCAA ACAGTTGTCA 
GAGGGTGCGT CTGTTGAAAC AATTGGCCGA GCATATCATG CAACGGAGGC ATTTGTGCAG 
GGTTTGTCGT ATGCAAAATC ATTGCTGAGA AAGCTTCTAG GTACCACTGA ATCGACCAAT 
AACAATGGTG AAAGAAGTAG AGATGTTGAT GATATATCTT CTGATGCTGG CAGCTCCAGT 
GTTGGCAGTC AGTCAACGGA TGAACCATCG GATGTTCTCT CACTTACAGA AATCTGGTTG 
GGGCGCGCAG AGTTGCTGCA GAGCCTCTTA GGGTCTGGAA TTTCTACTTC TCTTGATGAC 
ATTGCTGATC AATTGTCATC CGAATGTCTA CGAGACAGAT TAATATCTGA TGAACGATAT 
AGTATGGCGG TGTATATGTG CAAGAAATGC AAGATTGATG TTTTCCCCGT GTGGAAAGCA 
TGGGGTCTTG CTTTGTTACG GATGGAGCGC TATGCTCAAG CTCGAGTCAA ATTCAAGCAA 
GCCTTTCAAT TAAAGGGGGA AGACATTCCT GATGTCATTC AGGAGATAAT AAATACAATA 
GAAGGAGGTC CGCCTGTGGA TGTGTCAATT GTACGTTCCA TGTATGACCA TTTGGCGAAA 
AGCGCACCTA CAATTTTGGA CGATTCTTTA TCAGCAGACT CATACCTAAA TGTTCTGCAT 
ATGCCATCCA CTTTCCCTCG TTCAGAGAGG TCGCGGAGAT CTCTGGAATC GGAAAAGAAT 
AGTTCTGTAC CTGGTTCAGA CTTTGAAGAT GGGCCCCGAA GCAACTTGGA TACTACACGC 
TATTCTGAGT GCACCAACTA CTTGCAGGAA CATGCTCGCC AAAACCTGCT TGGGTTTATG 
TTCCGCCATG GTCACTTTAA AGATGCATGC ATGTTATTCT TTCCGCAAAG TGGTCTTCCC 
CCTCCTTTGC AAACTTCATC TGTGGGCGCA GTAAGCACAT CTTCATCACC TCAACGAACT 
GATCCCTTGG CAACTGAATA TGGGACCATT GAAAGTTTGT GCGAGTTCTG TGTTGGTTAT 
GGAGCTATCT CATCCCTTGA GGAAGTAATT ACAGAAAGAC TTGAATCCGC AAAGAATCAA 
GATCAAGCCA TAAATCAGTA CATAGCTGGA GCTCTTACTC GTATCTGTGC TTTCTTTGAG 
ATCAACCGGC ATTTCAATTA CCTATACAAG TTTCTGGTAC TCAAGAAGGA TTATGTCACC 
TCTGGGTATT GTTGTATTCA GCTTTTTATG AATTCTACAA CTCAGGAGGA TGCTGTAAGG 
CATCTTGAGC ATGCAAAGAA ATACTGGTCT CTAACTATCC TCGGGGTACA GGCACACTTT 
GAAGAAGCAT TGACAGCGCG TCATAGAGGT TCAGACTCAA AAAAACTTGT TACAAAGGGT 
GTTAGAGGAA AAAGTGCCGC AGAGAAGCTG AGTGAAGAAA CTCTTGTTAA GTTGTCCTCA 
CGGGTGAAAA TGCAGATTGA TGTGGTGAAG TCCTTCAGTG ACTCTGAAGG AGCACCATGG 
AAGCATTCCT TGTTTGGAAA TCCAAATGAT TCAGAGACAT CCAGGAGAAG ATGTGAAATA 
GTGGAGACTC TTGTTGAGAA AAATTTCGAC TTAGCTTATT CTGTTATATA TGAATTCAAG 
CTCTCAGCTG TTGATATATA TGCTGGTGTT GCTACGTCAC TAGCTGATAG GAAGAAAGGC 
AGTCAGTTGA CAGAACTTTT CAAAAACATT AAGGGAACAA TCCAGGATGA TGACTGGGAT 
CAGGTCCTGG GTGCTGCCAT CAATATATAT GCCAACAAGC ACAAAGAGCG CCCTGACCGT 
CTCATCGACA TGTTAACAAG CAGCCATCGA AAGGTGCTGG CTTGTGTGGT ATGTGGCCGC 
CTGAAAAGCG CATTCCAGAT TGCATCTAAA AGCGGAAGCG TGGCTGATGT TCAATATGTA 
GCTCATCAAG CCTTACATGC CAATTCGCAC ACAGTACTCG ATATGTGCAA GCAATGGCTA 
GCTAAATACA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT2G25730 ( NM_001202673.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001189602.1 Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), mRNA.

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CloneID	OAb05592
Clone ID Related Accession (Same CDS sequence)	NM_001202673.2 , NM_001336017.1
Accession Version	NM_001202673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	zinc finger FYVE domain protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_001202673.1.

ATGGATAAAG AGACGGAGAT TCTCTCCCGT CTCGCGGCGA ACCACCTTCA TCTGGCTCAA 
TTCGAGCCAT TGAAGGCTAC GTTACTCGCT CTCAGGGTTC GTAACCCTGA CCTCGCACTC 
ACCATTCTCC AAACCATCGT CTCCAACGCT GGAAGATTCG ATAATGTCCT CTGGTCACGC 
TCTTGTCCTT CCCCGTCTCT TCTCTCGTTC CTCTCCACGA TTGAGCTTCT GAGATTCGAA 
AATCCTACTT CTCCTTGGGG ATTTGATTCA GAAACTCTAA GTTTGCGTGC CGATTTCTTG 
TTGATGGTTC AGGTTTTGAT CGATAGAGTT ACAGAGAGGA TTAAGGAAGA TGAGGAGAGT 
GAGGATGAAA ATTCTGGATT AGGGAATTGT TTAAGGGTGT TGCAAGGTGT TTTGGAGTTA 
GGTGTTGAGA GGTTGAAGTT TGTTGTTGAT ACTAGTAGTA GTGAAGGAAG TAATAAGATT 
GAGGAAGATG CAGTTGTGTC TTTGAGGAGT ATAGTATTGG ATTACTCTGA TGTTTTCGAT 
GCTTTATGTT GTAATATTCA GAGGCAACTT GCGGGTTGCG AGAGTTACGG TACATGTTTG 
GTTGAGGAAG TTCAGGGAGA AGAACAGAGA AAGGAGATGA ATGAGGCCAC ATGTATTGGT 
TCTCCGGAGC TGGATAACAT CAATGTGTTT GCTTTGATAC AGAGGAATGT TCAGTTAGCA 
CAGTTGGATG CTATGAAAAC AAAGTTGGAT GAAGGTGATG AGCGCGGGGC AGCTGATCGC 
ATTCGTTATC TTCACCTTGA TTATGGAGTA GAGAAAGAGA ACTATCATGC TGTTCTAAAA 
GCTCTCCTTT CAAGAGTTAT GGAGAAAAAG GATGAATATG GTGATTCCTG GCACATGGTG 
CGCCAGAACT TGCTGTTTAT GTATAAAGAA GCTCTCTCAT CGAATTGTGG AGATCTTGTT 
CAGATGATCC AGGGTATTCA AGATGATATG CTCCTCCCAC ATAGCCAACT ACATTTATCT 
CTCGACAATG AACAAATTCC ACTCCCTCTT GAATGTTTCC GGCGATATCT TGTAGACTTG 
AAAACTGAGA GAAATATAGA GGACAAAAGT TCTCCTATGA GCAGGGCAAT TAATTCTTGT 
CTCAGAGATA TGTATCATTA TGCTCGTATT TCTGGATCAC ATGTTCTTGA GTGTGTGATG 
TGTGCTGCTT TGTCTTCTGT AAAGAAAGAG AAGCTTCAGG AGGCTAATGA TGTTCTTACT 
TTGTTTCCCC GACTTCGCCC TTTAGTAGCC TCCATGGGTT GGGATCTATT GCCGGGCAAA 
ACTGCAACCC GTAGAAAATT GATGCGGCTA CTTTGGACTA GTGACTCGCA AGCACTTCGG 
CTAGAAGAAT CTTCTCTTTA TGGAAACCAG ACAGATGAAA AATCTTGTGT GGAACACCTC 
TGCGATACGT TATGTTATCA GCTGGAACTT GCATCTTTCG CTGCTTGTGT CAATTCTGGT 
AAATCATGGA CTCCAAAGGC ATCTTTCTTG ATGCATGGTA ATGTGTCATC CGCGCATGAT 
GATGCGGAGG TGGATCCTTT TGTTGAAAAT CTTGTATTGG AAAGGCTTTC AGCGCAAAGT 
CCACTTCGGG TATTGTTTGA CGTTGTTCCG GGCATAAAAT TCCAAGATGC TATTTCACTG 
ATTAGTATGC AACCTATTGC TTCAACTGCA GAAGCCTGGA AGAGGATAGA AGATATTGAA 
CTGATGCATA TGCGTTATGC TCTGGAGGCA ATCGTTTTAG CACTAGGTGC AATGGAAGAG 
GCTATGAAGG ATGAGACAGA TGCTAGTCAT CGAGTAGTAT TTTACCATTT AAAAGACCTC 
ACTAACCATT TGGAGGCCAT TAAAAATGTT CCACGCAAGA TAATGATGGT GAACATAGTT 
ATTTCACTCT TACATATTGA TGATATCCGT CTCAGTTCTA CGCAAAGTGC CTCCTCGGCA 
TGTTTTTCTG AAAAAAGTAA CACACCTGGT TTGGATCCTG GCGATCTTGG TACAGAAGGG 
GAAAAGGAAA TTGTTATTTC TTTCACAAAA CAGCTACTCG ATGTTTTACG CCGCAATCTT 
CCATCACATC CAATTGAACA AGAGTGTCAG CTGGATGGTA ATTACAGTAC TGATGGAAGA 
CAGGCTTTAG AATGGAGAGT ATCCATGGCT AAGCGTTTCA TTGAAGATTG TGAATGGCGA 
TTATCTGTTA TGCAGCATCT TCTGCCACTT TCTGAACGCC AGTGGGGTTT AAAGGAGGTT 
TTGAGTATTC TAAGGGCAGC CCCTGAAAAA CTGCTTAATC TCTGTATGCA AAGAGCTAAG 
TATGACATTG GAGAAGAGGC AGTTAATCGG TTTGCGTTAT CAGCAGAGGA CAAAGCTACT 
CTTGAATTAG CTGAATGGGT TGATAATGCG TTCAAAGGAA CACTGGTAGA AGATGTAATG 
TCTCGTACTG CTGAAGGAGC AGCTGCCGTG CAAGATTTAG ATTTTCATTC TTTAGGTTCT 
CAATTGAGTC CATTGGCTAT GATTCTACTG TGCATAGATG CGGCTGCTTC TTCTGCCAAG 
TCTCCAACAA TTTCCAAACA ACTTTTGGAT AAGTCTCAAG TTATGTTATC GGAAATTTAC 
CCTGGAGGAG CTCCGAAGGT GGGGTTTACT TACTGGGATC AGGTCCACGA AGTTGCAATA 
ATTTCTGTAT TGCGAAGGAT CTTAAAGCGT CTGCAGGAAT TCCTTGAACA GGATGACCCT 
CAAATTCTTC AAGCCAGTTT TAGTGGAGAT ACCATAATTT CATCTTGCAC GGAATCTCAT 
AGACAGGGAC AAAAAGATCG TGCTCTTGCA ATGCTACATC AAATGATTGA GGATGCTCAT 
AGGGGCAAGC GTCAGTTCCT GAGTGGTAAG CTTCATAACT TAGCGAGAGC ACTCGCTGAT 
GAAAAACCAG AAGTTGACGT ACTCAAAGGG GACGGATCAG ACATGGCCGT TGAGAAGGAT 
GGAGTTCTTG GTCTTGGGCT AAAATATACA AAGCAAAGTC CTGGTTCAGC AAATAGAGCC 
GTGGATGGAA ATCCTGTTTC ACATGAAACA GAAGACAAGG GAAAGAAGTC ATTTGGCCCA 
TTAAGCAACA AAACCTCTAC TTATCTATCT CAGTTTATCC TCTATACTGC TGCTATTGGT 
GATATAGTAG ATGGAACTGA CACAACCCAT GATTTCAACT TTTTCTCTCT TGTTTATGAA 
TGGCCTAAAG ACCTATTGAC GCGTCTGGTT TTTGATCGAA GTAGCACAGA TGCAGCTGCA 
AAAGTTGCTG AGGTTATGTC TGCTGATTTT GTTCATGAAG TGATATCAGC ATGTGTTCCC 
CCAGTTTATC CCCCACGTTC TGGTCATGGG TGGGCTTGTA TTCCCGTCAT TCCAACCACT 
CCATGTTCCC ACTCAGAGGG TAAAGTGCTC TCTCCTTCAA TAGAGGCTAA ACCCAACTGT 
TATGTCCGTT CCTCAGCAAC ACCTGGTGTC CCTCTGTATC CTCTTCAGTT GGATGTTATC 
AGGCATTTGG TAAAAATTTC ACCAGTACGA GCAGTTTTAG CTTGCGTCTT TGGTGGGAGC 
ATATTGTACA ATGGCAGTGA TTCTATCATA TCTAGCTCCT TGAACGATGA GTTTCCAAGT 
TCTCCTGATG CAGACAGATT GTTTTATGAA TTTTCTCTTG ATCAGTCTGA GAGGTATCCC 
ACTTTAAACC GATGGATACA GATGCAGACT AATCTGCATC GAGTTTCGGA ATTTGTTGTG 
ACACCGAAGC AAAAACCTGA TGACACACGG ATTAAGCCTG ATGAAAGAAC TGGGATCAAG 
AGACTTCTTG AACATGATAG TGACTCAGAG TCAGATACAG AAGAAACATT TTCTAAAAAT 
AACATTCAAC CAGCATTGAC AGACGGCAGT GCTCGTGATG GTGGATCCTT TGAAAATGGA 
GTTTGTAGAA CTGATCCTAC CGTTTTCCTT TCTTTTGATT GGGAGAATGA AGTACCGTAT 
GAGAAAGCTG TAAATAGACT AATTGATGAA GGAAAACTAA TGGATGCTTT AGCACTTTCG 
GACCGCTTCT TGCGGAATGG GGCTTCTGAT TGGTTACTTC AGCTCCTAAT CAAAAGTAGA 
GAAGAAAATC CTTCAACATC AGGACGATCT CAGGGATATG GAGGCCAGAG CAACAGTTGG 
CAGTATTGCC TGCGGCTAAA GGACAAACAG CTGGCAGCAA CACTGGCTCT TAAGTATATG 
CATACGTGGG AGCTTGAGGC AGCTTTAAAT GTTCTTACAA TGTGTAGTTG TCATCTCTTA 
GAAAGCGATC CAATTAGTCT TGAGGTGTTG CATAGGAGAC AAGCTCTGCA GAAGTACAGC 
CACATACTTT CGGCAGATGA TCGCCATAAT AGCTGGCAAG AGGTAGAAGC CGAATGTAAA 
GAAGACCCTG AAGGCTTGGC TCTAAGATTG GCTGGGAAAG GAGCTGTTTC CGCTGCGCTA 
GAAGTGGCTG AAAGTGCAGG ATTATCAATA GATCTTAGAA GAGAATTACA AGGACGGCAG 
CTTGTAAAGC TTCTAACCAC TGATCCACTC AATGGTGGTG GTCCGGCGGA AGCATCTCGG 
TTTTTATCTT CACTTCAAGA CTCGGCTGAT GCTCTACCAG TGGTTATGGG TGCAATGCAA 
TTATTACCTG ACCTTCGGTC GAAACAGCTC CTGGTCCATT TCTTTCTCAA GCGAAGAGAT 
AGCAATCTGT CGGATTTGGA AGTCGCCCGG CTTAATTCTT GGGCTTTAGG TCTGAAAGTG 
TTAGCTGCAT TACCACTTCC TTGGCAACAG AGATGCTCTT CGCTTCATGA GCATCCCAAT 
TTAATATTTG AAGCTCTGCT AATGAGAAAA CAATTACAAT ACGCCTCACT GATACTCAAA 
GAGTTCCCAG CATTAAGAGA TAACAATGTT ATCATGGCCT ATGCAGCAAA AGCTATTTCT 
GTGACAATTA TCCCACCACC AAGAGAACCT CGAATAACTG TGTCTGCGTC AAGGTTAAGA 
CAGAAATCAA GAGCAGGGCC AGCAGTAAAA GCATCCTTCA CTAGTAGCTT AAGCAATTTT 
CAGAGAGAGG CTCGAAGGGC ATTCTCATGG GCTCCACGTA ATGCTGAAAA CCGGACGACG 
TCAAAGGATG TTTATCGCAA AAGAAAGAAT TCCGGACTGG GGGCTTCTGA GAGAGCTGCA 
TGGGAGGCGA TGACAGGTAT TCAAGAGGAC CAGGGATCAT CTTATTCGGC AGATGGGCAA 
GATAGGCTAC CTTCTGTTTC TATTGCTGAA GAATGGATGC TAACTGGCGA CAAAACCAAA 
GATGAAGGTG TTCGTGCATC TCACAAGTAT GAAAGCACCC CCGATATTAT TCTCTTTAAG 
GCTCTACTAT CACTTTGTTC AGATGAGCTA GTATCAGCAA GAAGTGCCAT GGACCTATGC 
ATCAGTCAAA TGAAAAACGT ATTGAGCTCC AAACAGTTGT CAGAGGGTGC GTCTGTTGAA 
ACAATTGGCC GAGCATATCA TGCAACGGAG GCATTTGTGC AGGGTTTGTC GTATGCAAAA 
TCATTGCTGA GAAAGCTTCT AGGTACCACT GAATCGACCA ATAACAATGG TGAAAGAAGT 
AGAGATGTTG ATGATATATC TTCTGATGCT GGCAGCTCCA GTGTTGGCAG TCAGTCAACG 
GATGAACCAT CGGATGTTCT CTCACTTACA GAAATCTGGT TGGGGCGCGC AGAGTTGCTG 
CAGAGCCTCT TAGGGTCTGG AATTTCTACT TCTCTTGATG ACATTGCTGA TCAATTGTCA 
TCCGAATGTC TACGAGACAG ATTAATATCT GATGAACGAT ATAGTATGGC GGTGTATATG 
TGCAAGAAAT GCAAGATTGA TGTTTTCCCC GTGTGGAAAG CATGGGGTCT TGCTTTGTTA 
CGGATGGAGC GCTATGCTCA AGCTCGAGTC AAATTCAAGC AAGCCTTTCA ATTAAAGGGG 
GAAGACATTC CTGATGTCAT TCAGGAGATA ATAAATACAA TAGAAGGAGG TCCGCCTGTG 
GATGTGTCAA TTGTACGTTC CATGTATGAC CATTTGGCGA AAAGCGCACC TACAATTTTG 
GACGATTCTT TATCAGCAGA CTCATACCTA AATGTTCTGC ATATGCCATC CACTTTCCCT 
CGTTCAGAGA GGTCGCGGAG ATCTCTGGAA TCGGAAAAGA ATAGTTCTGT ACCTGGTTCA 
GACTTTGAAG ATGGGCCCCG AAGCAACTTG GATACTACAC GCTATTCTGA GTGCACCAAC 
TACTTGCAGG AACATGCTCG CCAAAACCTG CTTGGGTTTA TGTTCCGCCA TGGTCACTTT 
AAAGATGCAT GCATGTTATT CTTTCCGCAA AGTGGTCTTC CCCCTCCTTT GCAAACTTCA 
TCTGTGGGCG CAGTAAGCAC ATCTTCATCA CCTCAACGAA CTGATCCCTT GGCAACTGAA 
TATGGGACCA TTGAAAGTTT GTGCGAGTTC TGTGTTGGTT ATGGAGCTAT CTCATCCCTT 
GAGGAAGTAA TTACAGAAAG ACTTGAATCC GCAAAGAATC AAGATCAAGC CATAAATCAG 
TACATAGCTG GAGCTCTTAC TCGTATCTGT GCTTTCTTTG AGATCAACCG GCATTTCAAT 
TACCTATACA AGTTTCTGGT ACTCAAGAAG GATTATGTCA CCTCTGGGTA TTGTTGTATT 
CAGCTTTTTA TGAATTCTAC AACTCAGGAG GATGCTGTAA GGCATCTTGA GCATGCAAAG 
GCACACTTTG AAGAAGCATT GACAGCGCGT CATAGAGGTT CAGACTCAAA AAAACTTGTT 
ACAAAGGGTG TTAGAGGAAA AAGTGCCGCA GAGAAGCTGA GTGAAGAAAC TCTTGTTAAG 
TTGTCCTCAC GGGTGAAAAT GCAGATTGAT GTGGTGAAGT CCTTCAGTGA CTCTGAAGGA 
GCACCATGGA AGCATTCCTT GTTTGGAAAT CCAAATGATT CAGAGACATC CAGGAGAAGA 
TGTGAAATAG TGGAGACTCT TGTTGAGAAA AATTTCGACT TAGCTTATTC TGTTATATAT 
GAATTCAAGC TCTCAGCTGT TGATATATAT GCTGGTGTTG CTACGTCACT AGCTGATAGG 
AAGAAAGGCA GTCAGTTGAC AGAACTTTTC AAAAACATTA AGGGAACAAT CCAGGATGAT 
GACTGGGATC AGGTCCTGGG TGCTGCCATC AATATATATG CCAACAAGCA CAAAGAGCGC 
CCTGACCGTC TCATCGACAT GTTAACAAGC AGCCATCGAA AGGTGCTGGC TTGTGTGGTA 
TGTGGCCGCC TGAAAAGCGC ATTCCAGATT GCATCTAAAA GCGGAAGCGT GGCTGATGTT 
CAATATGTAG CTCATCAAGC CTTACATGCC AATTCGCACA CAGTACTCGA TATGTGCAAG 
CAATGGCTAG CTAAATACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189602.1
CDS	223..7686
Translation	MDKETEILSRLAANHLHLAQFEPLKATLLALRVRNPDLALTILQTIVSNAGRFDNVLWSRSCPSPSLLSFLSTIELLRFENPTSPWGFDSETLSLRADFLLMVQVLIDRVTERIKEDEESEDENSGLGNCLRVLQGVLELGVERLKFVVDTSSSEGSNKIEEDAVVSLRSIVLDYSDVFDALCCNIQRQLAGCESYGTCLVEEVQGEEQRKEMNEATCIGSPELDNINVFALIQRNVQLAQLDAMKTKLDEGDERGAADRIRYLHLDYGVEKENYHAVLKALLSRVMEKKDEYGDSWHMVRQNLLFMYKEALSSNCGDLVQMIQGIQDDMLLPHSQLHLSLDNEQIPLPLECFRRYLVDLKTERNIEDKSSPMSRAINSCLRDMYHYARISGSHVLECVMCAALSSVKKEKLQEANDVLTLFPRLRPLVASMGWDLLPGKTATRRKLMRLLWTSDSQALRLEESSLYGNQTDEKSCVEHLCDTLCYQLELASFAACVNSGKSWTPKASFLMHGNVSSAHDDAEVDPFVENLVLERLSAQSPLRVLFDVVPGIKFQDAISLISMQPIASTAEAWKRIEDIELMHMRYALEAIVLALGAMEEAMKDETDASHRVVFYHLKDLTNHLEAIKNVPRKIMMVNIVISLLHIDDIRLSSTQSASSACFSEKSNTPGLDPGDLGTEGEKEIVISFTKQLLDVLRRNLPSHPIEQECQLDGNYSTDGRQALEWRVSMAKRFIEDCEWRLSVMQHLLPLSERQWGLKEVLSILRAAPEKLLNLCMQRAKYDIGEEAVNRFALSAEDKATLELAEWVDNAFKGTLVEDVMSRTAEGAAAVQDLDFHSLGSQLSPLAMILLCIDAAASSAKSPTISKQLLDKSQVMLSEIYPGGAPKVGFTYWDQVHEVAIISVLRRILKRLQEFLEQDDPQILQASFSGDTIISSCTESHRQGQKDRALAMLHQMIEDAHRGKRQFLSGKLHNLARALADEKPEVDVLKGDGSDMAVEKDGVLGLGLKYTKQSPGSANRAVDGNPVSHETEDKGKKSFGPLSNKTSTYLSQFILYTAAIGDIVDGTDTTHDFNFFSLVYEWPKDLLTRLVFDRSSTDAAAKVAEVMSADFVHEVISACVPPVYPPRSGHGWACIPVIPTTPCSHSEGKVLSPSIEAKPNCYVRSSATPGVPLYPLQLDVIRHLVKISPVRAVLACVFGGSILYNGSDSIISSSLNDEFPSSPDADRLFYEFSLDQSERYPTLNRWIQMQTNLHRVSEFVVTPKQKPDDTRIKPDERTGIKRLLEHDSDSESDTEETFSKNNIQPALTDGSARDGGSFENGVCRTDPTVFLSFDWENEVPYEKAVNRLIDEGKLMDALALSDRFLRNGASDWLLQLLIKSREENPSTSGRSQGYGGQSNSWQYCLRLKDKQLAATLALKYMHTWELEAALNVLTMCSCHLLESDPISLEVLHRRQALQKYSHILSADDRHNSWQEVEAECKEDPEGLALRLAGKGAVSAALEVAESAGLSIDLRRELQGRQLVKLLTTDPLNGGGPAEASRFLSSLQDSADALPVVMGAMQLLPDLRSKQLLVHFFLKRRDSNLSDLEVARLNSWALGLKVLAALPLPWQQRCSSLHEHPNLIFEALLMRKQLQYASLILKEFPALRDNNVIMAYAAKAISVTIIPPPREPRITVSASRLRQKSRAGPAVKASFTSSLSNFQREARRAFSWAPRNAENRTTSKDVYRKRKNSGLGASERAAWEAMTGIQEDQGSSYSADGQDRLPSVSIAEEWMLTGDKTKDEGVRASHKYESTPDIILFKALLSLCSDELVSARSAMDLCISQMKNVLSSKQLSEGASVETIGRAYHATEAFVQGLSYAKSLLRKLLGTTESTNNNGERSRDVDDISSDAGSSSVGSQSTDEPSDVLSLTEIWLGRAELLQSLLGSGISTSLDDIADQLSSECLRDRLISDERYSMAVYMCKKCKIDVFPVWKAWGLALLRMERYAQARVKFKQAFQLKGEDIPDVIQEIINTIEGGPPVDVSIVRSMYDHLAKSAPTILDDSLSADSYLNVLHMPSTFPRSERSRRSLESEKNSSVPGSDFEDGPRSNLDTTRYSECTNYLQEHARQNLLGFMFRHGHFKDACMLFFPQSGLPPPLQTSSVGAVSTSSSPQRTDPLATEYGTIESLCEFCVGYGAISSLEEVITERLESAKNQDQAINQYIAGALTRICAFFEINRHFNYLYKFLVLKKDYVTSGYCCIQLFMNSTTQEDAVRHLEHAKAHFEEALTARHRGSDSKKLVTKGVRGKSAAEKLSEETLVKLSSRVKMQIDVVKSFSDSEGAPWKHSLFGNPNDSETSRRRCEIVETLVEKNFDLAYSVIYEFKLSAVDIYAGVATSLADRKKGSQLTELFKNIKGTIQDDDWDQVLGAAINIYANKHKERPDRLIDMLTSSHRKVLACVVCGRLKSAFQIASKSGSVADVQYVAHQALHANSHTVLDMCKQWLAKYM

Target ORF information:

RefSeq Version	NM_001202673.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202673.2

ORF Insert Sequence:

ATGGATAAAG AGACGGAGAT TCTCTCCCGT CTCGCGGCGA ACCACCTTCA TCTGGCTCAA 
TTCGAGCCAT TGAAGGCTAC GTTACTCGCT CTCAGGGTTC GTAACCCTGA CCTCGCACTC 
ACCATTCTCC AAACCATCGT CTCCAACGCT GGAAGATTCG ATAATGTCCT CTGGTCACGC 
TCTTGTCCTT CCCCGTCTCT TCTCTCGTTC CTCTCCACGA TTGAGCTTCT GAGATTCGAA 
AATCCTACTT CTCCTTGGGG ATTTGATTCA GAAACTCTAA GTTTGCGTGC CGATTTCTTG 
TTGATGGTTC AGGTTTTGAT CGATAGAGTT ACAGAGAGGA TTAAGGAAGA TGAGGAGAGT 
GAGGATGAAA ATTCTGGATT AGGGAATTGT TTAAGGGTGT TGCAAGGTGT TTTGGAGTTA 
GGTGTTGAGA GGTTGAAGTT TGTTGTTGAT ACTAGTAGTA GTGAAGGAAG TAATAAGATT 
GAGGAAGATG CAGTTGTGTC TTTGAGGAGT ATAGTATTGG ATTACTCTGA TGTTTTCGAT 
GCTTTATGTT GTAATATTCA GAGGCAACTT GCGGGTTGCG AGAGTTACGG TACATGTTTG 
GTTGAGGAAG TTCAGGGAGA AGAACAGAGA AAGGAGATGA ATGAGGCCAC ATGTATTGGT 
TCTCCGGAGC TGGATAACAT CAATGTGTTT GCTTTGATAC AGAGGAATGT TCAGTTAGCA 
CAGTTGGATG CTATGAAAAC AAAGTTGGAT GAAGGTGATG AGCGCGGGGC AGCTGATCGC 
ATTCGTTATC TTCACCTTGA TTATGGAGTA GAGAAAGAGA ACTATCATGC TGTTCTAAAA 
GCTCTCCTTT CAAGAGTTAT GGAGAAAAAG GATGAATATG GTGATTCCTG GCACATGGTG 
CGCCAGAACT TGCTGTTTAT GTATAAAGAA GCTCTCTCAT CGAATTGTGG AGATCTTGTT 
CAGATGATCC AGGGTATTCA AGATGATATG CTCCTCCCAC ATAGCCAACT ACATTTATCT 
CTCGACAATG AACAAATTCC ACTCCCTCTT GAATGTTTCC GGCGATATCT TGTAGACTTG 
AAAACTGAGA GAAATATAGA GGACAAAAGT TCTCCTATGA GCAGGGCAAT TAATTCTTGT 
CTCAGAGATA TGTATCATTA TGCTCGTATT TCTGGATCAC ATGTTCTTGA GTGTGTGATG 
TGTGCTGCTT TGTCTTCTGT AAAGAAAGAG AAGCTTCAGG AGGCTAATGA TGTTCTTACT 
TTGTTTCCCC GACTTCGCCC TTTAGTAGCC TCCATGGGTT GGGATCTATT GCCGGGCAAA 
ACTGCAACCC GTAGAAAATT GATGCGGCTA CTTTGGACTA GTGACTCGCA AGCACTTCGG 
CTAGAAGAAT CTTCTCTTTA TGGAAACCAG ACAGATGAAA AATCTTGTGT GGAACACCTC 
TGCGATACGT TATGTTATCA GCTGGAACTT GCATCTTTCG CTGCTTGTGT CAATTCTGGT 
AAATCATGGA CTCCAAAGGC ATCTTTCTTG ATGCATGGTA ATGTGTCATC CGCGCATGAT 
GATGCGGAGG TGGATCCTTT TGTTGAAAAT CTTGTATTGG AAAGGCTTTC AGCGCAAAGT 
CCACTTCGGG TATTGTTTGA CGTTGTTCCG GGCATAAAAT TCCAAGATGC TATTTCACTG 
ATTAGTATGC AACCTATTGC TTCAACTGCA GAAGCCTGGA AGAGGATAGA AGATATTGAA 
CTGATGCATA TGCGTTATGC TCTGGAGGCA ATCGTTTTAG CACTAGGTGC AATGGAAGAG 
GCTATGAAGG ATGAGACAGA TGCTAGTCAT CGAGTAGTAT TTTACCATTT AAAAGACCTC 
ACTAACCATT TGGAGGCCAT TAAAAATGTT CCACGCAAGA TAATGATGGT GAACATAGTT 
ATTTCACTCT TACATATTGA TGATATCCGT CTCAGTTCTA CGCAAAGTGC CTCCTCGGCA 
TGTTTTTCTG AAAAAAGTAA CACACCTGGT TTGGATCCTG GCGATCTTGG TACAGAAGGG 
GAAAAGGAAA TTGTTATTTC TTTCACAAAA CAGCTACTCG ATGTTTTACG CCGCAATCTT 
CCATCACATC CAATTGAACA AGAGTGTCAG CTGGATGGTA ATTACAGTAC TGATGGAAGA 
CAGGCTTTAG AATGGAGAGT ATCCATGGCT AAGCGTTTCA TTGAAGATTG TGAATGGCGA 
TTATCTGTTA TGCAGCATCT TCTGCCACTT TCTGAACGCC AGTGGGGTTT AAAGGAGGTT 
TTGAGTATTC TAAGGGCAGC CCCTGAAAAA CTGCTTAATC TCTGTATGCA AAGAGCTAAG 
TATGACATTG GAGAAGAGGC AGTTAATCGG TTTGCGTTAT CAGCAGAGGA CAAAGCTACT 
CTTGAATTAG CTGAATGGGT TGATAATGCG TTCAAAGGAA CACTGGTAGA AGATGTAATG 
TCTCGTACTG CTGAAGGAGC AGCTGCCGTG CAAGATTTAG ATTTTCATTC TTTAGGTTCT 
CAATTGAGTC CATTGGCTAT GATTCTACTG TGCATAGATG CGGCTGCTTC TTCTGCCAAG 
TCTCCAACAA TTTCCAAACA ACTTTTGGAT AAGTCTCAAG TTATGTTATC GGAAATTTAC 
CCTGGAGGAG CTCCGAAGGT GGGGTTTACT TACTGGGATC AGGTCCACGA AGTTGCAATA 
ATTTCTGTAT TGCGAAGGAT CTTAAAGCGT CTGCAGGAAT TCCTTGAACA GGATGACCCT 
CAAATTCTTC AAGCCAGTTT TAGTGGAGAT ACCATAATTT CATCTTGCAC GGAATCTCAT 
AGACAGGGAC AAAAAGATCG TGCTCTTGCA ATGCTACATC AAATGATTGA GGATGCTCAT 
AGGGGCAAGC GTCAGTTCCT GAGTGGTAAG CTTCATAACT TAGCGAGAGC ACTCGCTGAT 
GAAAAACCAG AAGTTGACGT ACTCAAAGGG GACGGATCAG ACATGGCCGT TGAGAAGGAT 
GGAGTTCTTG GTCTTGGGCT AAAATATACA AAGCAAAGTC CTGGTTCAGC AAATAGAGCC 
GTGGATGGAA ATCCTGTTTC ACATGAAACA GAAGACAAGG GAAAGAAGTC ATTTGGCCCA 
TTAAGCAACA AAACCTCTAC TTATCTATCT CAGTTTATCC TCTATACTGC TGCTATTGGT 
GATATAGTAG ATGGAACTGA CACAACCCAT GATTTCAACT TTTTCTCTCT TGTTTATGAA 
TGGCCTAAAG ACCTATTGAC GCGTCTGGTT TTTGATCGAA GTAGCACAGA TGCAGCTGCA 
AAAGTTGCTG AGGTTATGTC TGCTGATTTT GTTCATGAAG TGATATCAGC ATGTGTTCCC 
CCAGTTTATC CCCCACGTTC TGGTCATGGG TGGGCTTGTA TTCCCGTCAT TCCAACCACT 
CCATGTTCCC ACTCAGAGGG TAAAGTGCTC TCTCCTTCAA TAGAGGCTAA ACCCAACTGT 
TATGTCCGTT CCTCAGCAAC ACCTGGTGTC CCTCTGTATC CTCTTCAGTT GGATGTTATC 
AGGCATTTGG TAAAAATTTC ACCAGTACGA GCAGTTTTAG CTTGCGTCTT TGGTGGGAGC 
ATATTGTACA ATGGCAGTGA TTCTATCATA TCTAGCTCCT TGAACGATGA GTTTCCAAGT 
TCTCCTGATG CAGACAGATT GTTTTATGAA TTTTCTCTTG ATCAGTCTGA GAGGTATCCC 
ACTTTAAACC GATGGATACA GATGCAGACT AATCTGCATC GAGTTTCGGA ATTTGTTGTG 
ACACCGAAGC AAAAACCTGA TGACACACGG ATTAAGCCTG ATGAAAGAAC TGGGATCAAG 
AGACTTCTTG AACATGATAG TGACTCAGAG TCAGATACAG AAGAAACATT TTCTAAAAAT 
AACATTCAAC CAGCATTGAC AGACGGCAGT GCTCGTGATG GTGGATCCTT TGAAAATGGA 
GTTTGTAGAA CTGATCCTAC CGTTTTCCTT TCTTTTGATT GGGAGAATGA AGTACCGTAT 
GAGAAAGCTG TAAATAGACT AATTGATGAA GGAAAACTAA TGGATGCTTT AGCACTTTCG 
GACCGCTTCT TGCGGAATGG GGCTTCTGAT TGGTTACTTC AGCTCCTAAT CAAAAGTAGA 
GAAGAAAATC CTTCAACATC AGGACGATCT CAGGGATATG GAGGCCAGAG CAACAGTTGG 
CAGTATTGCC TGCGGCTAAA GGACAAACAG CTGGCAGCAA CACTGGCTCT TAAGTATATG 
CATACGTGGG AGCTTGAGGC AGCTTTAAAT GTTCTTACAA TGTGTAGTTG TCATCTCTTA 
GAAAGCGATC CAATTAGTCT TGAGGTGTTG CATAGGAGAC AAGCTCTGCA GAAGTACAGC 
CACATACTTT CGGCAGATGA TCGCCATAAT AGCTGGCAAG AGGTAGAAGC CGAATGTAAA 
GAAGACCCTG AAGGCTTGGC TCTAAGATTG GCTGGGAAAG GAGCTGTTTC CGCTGCGCTA 
GAAGTGGCTG AAAGTGCAGG ATTATCAATA GATCTTAGAA GAGAATTACA AGGACGGCAG 
CTTGTAAAGC TTCTAACCAC TGATCCACTC AATGGTGGTG GTCCGGCGGA AGCATCTCGG 
TTTTTATCTT CACTTCAAGA CTCGGCTGAT GCTCTACCAG TGGTTATGGG TGCAATGCAA 
TTATTACCTG ACCTTCGGTC GAAACAGCTC CTGGTCCATT TCTTTCTCAA GCGAAGAGAT 
AGCAATCTGT CGGATTTGGA AGTCGCCCGG CTTAATTCTT GGGCTTTAGG TCTGAAAGTG 
TTAGCTGCAT TACCACTTCC TTGGCAACAG AGATGCTCTT CGCTTCATGA GCATCCCAAT 
TTAATATTTG AAGCTCTGCT AATGAGAAAA CAATTACAAT ACGCCTCACT GATACTCAAA 
GAGTTCCCAG CATTAAGAGA TAACAATGTT ATCATGGCCT ATGCAGCAAA AGCTATTTCT 
GTGACAATTA TCCCACCACC AAGAGAACCT CGAATAACTG TGTCTGCGTC AAGGTTAAGA 
CAGAAATCAA GAGCAGGGCC AGCAGTAAAA GCATCCTTCA CTAGTAGCTT AAGCAATTTT 
CAGAGAGAGG CTCGAAGGGC ATTCTCATGG GCTCCACGTA ATGCTGAAAA CCGGACGACG 
TCAAAGGATG TTTATCGCAA AAGAAAGAAT TCCGGACTGG GGGCTTCTGA GAGAGCTGCA 
TGGGAGGCGA TGACAGGTAT TCAAGAGGAC CAGGGATCAT CTTATTCGGC AGATGGGCAA 
GATAGGCTAC CTTCTGTTTC TATTGCTGAA GAATGGATGC TAACTGGCGA CAAAACCAAA 
GATGAAGGTG TTCGTGCATC TCACAAGTAT GAAAGCACCC CCGATATTAT TCTCTTTAAG 
GCTCTACTAT CACTTTGTTC AGATGAGCTA GTATCAGCAA GAAGTGCCAT GGACCTATGC 
ATCAGTCAAA TGAAAAACGT ATTGAGCTCC AAACAGTTGT CAGAGGGTGC GTCTGTTGAA 
ACAATTGGCC GAGCATATCA TGCAACGGAG GCATTTGTGC AGGGTTTGTC GTATGCAAAA 
TCATTGCTGA GAAAGCTTCT AGGTACCACT GAATCGACCA ATAACAATGG TGAAAGAAGT 
AGAGATGTTG ATGATATATC TTCTGATGCT GGCAGCTCCA GTGTTGGCAG TCAGTCAACG 
GATGAACCAT CGGATGTTCT CTCACTTACA GAAATCTGGT TGGGGCGCGC AGAGTTGCTG 
CAGAGCCTCT TAGGGTCTGG AATTTCTACT TCTCTTGATG ACATTGCTGA TCAATTGTCA 
TCCGAATGTC TACGAGACAG ATTAATATCT GATGAACGAT ATAGTATGGC GGTGTATATG 
TGCAAGAAAT GCAAGATTGA TGTTTTCCCC GTGTGGAAAG CATGGGGTCT TGCTTTGTTA 
CGGATGGAGC GCTATGCTCA AGCTCGAGTC AAATTCAAGC AAGCCTTTCA ATTAAAGGGG 
GAAGACATTC CTGATGTCAT TCAGGAGATA ATAAATACAA TAGAAGGAGG TCCGCCTGTG 
GATGTGTCAA TTGTACGTTC CATGTATGAC CATTTGGCGA AAAGCGCACC TACAATTTTG 
GACGATTCTT TATCAGCAGA CTCATACCTA AATGTTCTGC ATATGCCATC CACTTTCCCT 
CGTTCAGAGA GGTCGCGGAG ATCTCTGGAA TCGGAAAAGA ATAGTTCTGT ACCTGGTTCA 
GACTTTGAAG ATGGGCCCCG AAGCAACTTG GATACTACAC GCTATTCTGA GTGCACCAAC 
TACTTGCAGG AACATGCTCG CCAAAACCTG CTTGGGTTTA TGTTCCGCCA TGGTCACTTT 
AAAGATGCAT GCATGTTATT CTTTCCGCAA AGTGGTCTTC CCCCTCCTTT GCAAACTTCA 
TCTGTGGGCG CAGTAAGCAC ATCTTCATCA CCTCAACGAA CTGATCCCTT GGCAACTGAA 
TATGGGACCA TTGAAAGTTT GTGCGAGTTC TGTGTTGGTT ATGGAGCTAT CTCATCCCTT 
GAGGAAGTAA TTACAGAAAG ACTTGAATCC GCAAAGAATC AAGATCAAGC CATAAATCAG 
TACATAGCTG GAGCTCTTAC TCGTATCTGT GCTTTCTTTG AGATCAACCG GCATTTCAAT 
TACCTATACA AGTTTCTGGT ACTCAAGAAG GATTATGTCA CCTCTGGGTA TTGTTGTATT 
CAGCTTTTTA TGAATTCTAC AACTCAGGAG GATGCTGTAA GGCATCTTGA GCATGCAAAG 
GCACACTTTG AAGAAGCATT GACAGCGCGT CATAGAGGTT CAGACTCAAA AAAACTTGTT 
ACAAAGGGTG TTAGAGGAAA AAGTGCCGCA GAGAAGCTGA GTGAAGAAAC TCTTGTTAAG 
TTGTCCTCAC GGGTGAAAAT GCAGATTGAT GTGGTGAAGT CCTTCAGTGA CTCTGAAGGA 
GCACCATGGA AGCATTCCTT GTTTGGAAAT CCAAATGATT CAGAGACATC CAGGAGAAGA 
TGTGAAATAG TGGAGACTCT TGTTGAGAAA AATTTCGACT TAGCTTATTC TGTTATATAT 
GAATTCAAGC TCTCAGCTGT TGATATATAT GCTGGTGTTG CTACGTCACT AGCTGATAGG 
AAGAAAGGCA GTCAGTTGAC AGAACTTTTC AAAAACATTA AGGGAACAAT CCAGGATGAT 
GACTGGGATC AGGTCCTGGG TGCTGCCATC AATATATATG CCAACAAGCA CAAAGAGCGC 
CCTGACCGTC TCATCGACAT GTTAACAAGC AGCCATCGAA AGGTGCTGGC TTGTGTGGTA 
TGTGGCCGCC TGAAAAGCGC ATTCCAGATT GCATCTAAAA GCGGAAGCGT GGCTGATGTT 
CAATATGTAG CTCATCAAGC CTTACATGCC AATTCGCACA CAGTACTCGA TATGTGCAAG 
CAATGGCTAG CTAAATACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT2G25730 ( NM_001336017.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324903.1 Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), mRNA.

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CloneID	OAb05592
Clone ID Related Accession (Same CDS sequence)	NM_001202673.2 , NM_001336017.1
Accession Version	NM_001336017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	zinc finger FYVE domain protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGATAAAG AGACGGAGAT TCTCTCCCGT CTCGCGGCGA ACCACCTTCA TCTGGCTCAA 
TTCGAGCCAT TGAAGGCTAC GTTACTCGCT CTCAGGGTTC GTAACCCTGA CCTCGCACTC 
ACCATTCTCC AAACCATCGT CTCCAACGCT GGAAGATTCG ATAATGTCCT CTGGTCACGC 
TCTTGTCCTT CCCCGTCTCT TCTCTCGTTC CTCTCCACGA TTGAGCTTCT GAGATTCGAA 
AATCCTACTT CTCCTTGGGG ATTTGATTCA GAAACTCTAA GTTTGCGTGC CGATTTCTTG 
TTGATGGTTC AGGTTTTGAT CGATAGAGTT ACAGAGAGGA TTAAGGAAGA TGAGGAGAGT 
GAGGATGAAA ATTCTGGATT AGGGAATTGT TTAAGGGTGT TGCAAGGTGT TTTGGAGTTA 
GGTGTTGAGA GGTTGAAGTT TGTTGTTGAT ACTAGTAGTA GTGAAGGAAG TAATAAGATT 
GAGGAAGATG CAGTTGTGTC TTTGAGGAGT ATAGTATTGG ATTACTCTGA TGTTTTCGAT 
GCTTTATGTT GTAATATTCA GAGGCAACTT GCGGGTTGCG AGAGTTACGG TACATGTTTG 
GTTGAGGAAG TTCAGGGAGA AGAACAGAGA AAGGAGATGA ATGAGGCCAC ATGTATTGGT 
TCTCCGGAGC TGGATAACAT CAATGTGTTT GCTTTGATAC AGAGGAATGT TCAGTTAGCA 
CAGTTGGATG CTATGAAAAC AAAGTTGGAT GAAGGTGATG AGCGCGGGGC AGCTGATCGC 
ATTCGTTATC TTCACCTTGA TTATGGAGTA GAGAAAGAGA ACTATCATGC TGTTCTAAAA 
GCTCTCCTTT CAAGAGTTAT GGAGAAAAAG GATGAATATG GTGATTCCTG GCACATGGTG 
CGCCAGAACT TGCTGTTTAT GTATAAAGAA GCTCTCTCAT CGAATTGTGG AGATCTTGTT 
CAGATGATCC AGGGTATTCA AGATGATATG CTCCTCCCAC ATAGCCAACT ACATTTATCT 
CTCGACAATG AACAAATTCC ACTCCCTCTT GAATGTTTCC GGCGATATCT TGTAGACTTG 
AAAACTGAGA GAAATATAGA GGACAAAAGT TCTCCTATGA GCAGGGCAAT TAATTCTTGT 
CTCAGAGATA TGTATCATTA TGCTCGTATT TCTGGATCAC ATGTTCTTGA GTGTGTGATG 
TGTGCTGCTT TGTCTTCTGT AAAGAAAGAG AAGCTTCAGG AGGCTAATGA TGTTCTTACT 
TTGTTTCCCC GACTTCGCCC TTTAGTAGCC TCCATGGGTT GGGATCTATT GCCGGGCAAA 
ACTGCAACCC GTAGAAAATT GATGCGGCTA CTTTGGACTA GTGACTCGCA AGCACTTCGG 
CTAGAAGAAT CTTCTCTTTA TGGAAACCAG ACAGATGAAA AATCTTGTGT GGAACACCTC 
TGCGATACGT TATGTTATCA GCTGGAACTT GCATCTTTCG CTGCTTGTGT CAATTCTGGT 
AAATCATGGA CTCCAAAGGC ATCTTTCTTG ATGCATGGTA ATGTGTCATC CGCGCATGAT 
GATGCGGAGG TGGATCCTTT TGTTGAAAAT CTTGTATTGG AAAGGCTTTC AGCGCAAAGT 
CCACTTCGGG TATTGTTTGA CGTTGTTCCG GGCATAAAAT TCCAAGATGC TATTTCACTG 
ATTAGTATGC AACCTATTGC TTCAACTGCA GAAGCCTGGA AGAGGATAGA AGATATTGAA 
CTGATGCATA TGCGTTATGC TCTGGAGGCA ATCGTTTTAG CACTAGGTGC AATGGAAGAG 
GCTATGAAGG ATGAGACAGA TGCTAGTCAT CGAGTAGTAT TTTACCATTT AAAAGACCTC 
ACTAACCATT TGGAGGCCAT TAAAAATGTT CCACGCAAGA TAATGATGGT GAACATAGTT 
ATTTCACTCT TACATATTGA TGATATCCGT CTCAGTTCTA CGCAAAGTGC CTCCTCGGCA 
TGTTTTTCTG AAAAAAGTAA CACACCTGGT TTGGATCCTG GCGATCTTGG TACAGAAGGG 
GAAAAGGAAA TTGTTATTTC TTTCACAAAA CAGCTACTCG ATGTTTTACG CCGCAATCTT 
CCATCACATC CAATTGAACA AGAGTGTCAG CTGGATGGTA ATTACAGTAC TGATGGAAGA 
CAGGCTTTAG AATGGAGAGT ATCCATGGCT AAGCGTTTCA TTGAAGATTG TGAATGGCGA 
TTATCTGTTA TGCAGCATCT TCTGCCACTT TCTGAACGCC AGTGGGGTTT AAAGGAGGTT 
TTGAGTATTC TAAGGGCAGC CCCTGAAAAA CTGCTTAATC TCTGTATGCA AAGAGCTAAG 
TATGACATTG GAGAAGAGGC AGTTAATCGG TTTGCGTTAT CAGCAGAGGA CAAAGCTACT 
CTTGAATTAG CTGAATGGGT TGATAATGCG TTCAAAGGAA CACTGGTAGA AGATGTAATG 
TCTCGTACTG CTGAAGGAGC AGCTGCCGTG CAAGATTTAG ATTTTCATTC TTTAGGTTCT 
CAATTGAGTC CATTGGCTAT GATTCTACTG TGCATAGATG CGGCTGCTTC TTCTGCCAAG 
TCTCCAACAA TTTCCAAACA ACTTTTGGAT AAGTCTCAAG TTATGTTATC GGAAATTTAC 
CCTGGAGGAG CTCCGAAGGT GGGGTTTACT TACTGGGATC AGGTCCACGA AGTTGCAATA 
ATTTCTGTAT TGCGAAGGAT CTTAAAGCGT CTGCAGGAAT TCCTTGAACA GGATGACCCT 
CAAATTCTTC AAGCCAGTTT TAGTGGAGAT ACCATAATTT CATCTTGCAC GGAATCTCAT 
AGACAGGGAC AAAAAGATCG TGCTCTTGCA ATGCTACATC AAATGATTGA GGATGCTCAT 
AGGGGCAAGC GTCAGTTCCT GAGTGGTAAG CTTCATAACT TAGCGAGAGC ACTCGCTGAT 
GAAAAACCAG AAGTTGACGT ACTCAAAGGG GACGGATCAG ACATGGCCGT TGAGAAGGAT 
GGAGTTCTTG GTCTTGGGCT AAAATATACA AAGCAAAGTC CTGGTTCAGC AAATAGAGCC 
GTGGATGGAA ATCCTGTTTC ACATGAAACA GAAGACAAGG GAAAGAAGTC ATTTGGCCCA 
TTAAGCAACA AAACCTCTAC TTATCTATCT CAGTTTATCC TCTATACTGC TGCTATTGGT 
GATATAGTAG ATGGAACTGA CACAACCCAT GATTTCAACT TTTTCTCTCT TGTTTATGAA 
TGGCCTAAAG ACCTATTGAC GCGTCTGGTT TTTGATCGAA GTAGCACAGA TGCAGCTGCA 
AAAGTTGCTG AGGTTATGTC TGCTGATTTT GTTCATGAAG TGATATCAGC ATGTGTTCCC 
CCAGTTTATC CCCCACGTTC TGGTCATGGG TGGGCTTGTA TTCCCGTCAT TCCAACCACT 
CCATGTTCCC ACTCAGAGGG TAAAGTGCTC TCTCCTTCAA TAGAGGCTAA ACCCAACTGT 
TATGTCCGTT CCTCAGCAAC ACCTGGTGTC CCTCTGTATC CTCTTCAGTT GGATGTTATC 
AGGCATTTGG TAAAAATTTC ACCAGTACGA GCAGTTTTAG CTTGCGTCTT TGGTGGGAGC 
ATATTGTACA ATGGCAGTGA TTCTATCATA TCTAGCTCCT TGAACGATGA GTTTCCAAGT 
TCTCCTGATG CAGACAGATT GTTTTATGAA TTTTCTCTTG ATCAGTCTGA GAGGTATCCC 
ACTTTAAACC GATGGATACA GATGCAGACT AATCTGCATC GAGTTTCGGA ATTTGTTGTG 
ACACCGAAGC AAAAACCTGA TGACACACGG ATTAAGCCTG ATGAAAGAAC TGGGATCAAG 
AGACTTCTTG AACATGATAG TGACTCAGAG TCAGATACAG AAGAAACATT TTCTAAAAAT 
AACATTCAAC CAGCATTGAC AGACGGCAGT GCTCGTGATG GTGGATCCTT TGAAAATGGA 
GTTTGTAGAA CTGATCCTAC CGTTTTCCTT TCTTTTGATT GGGAGAATGA AGTACCGTAT 
GAGAAAGCTG TAAATAGACT AATTGATGAA GGAAAACTAA TGGATGCTTT AGCACTTTCG 
GACCGCTTCT TGCGGAATGG GGCTTCTGAT TGGTTACTTC AGCTCCTAAT CAAAAGTAGA 
GAAGAAAATC CTTCAACATC AGGACGATCT CAGGGATATG GAGGCCAGAG CAACAGTTGG 
CAGTATTGCC TGCGGCTAAA GGACAAACAG CTGGCAGCAA CACTGGCTCT TAAGTATATG 
CATACGTGGG AGCTTGAGGC AGCTTTAAAT GTTCTTACAA TGTGTAGTTG TCATCTCTTA 
GAAAGCGATC CAATTAGTCT TGAGGTGTTG CATAGGAGAC AAGCTCTGCA GAAGTACAGC 
CACATACTTT CGGCAGATGA TCGCCATAAT AGCTGGCAAG AGGTAGAAGC CGAATGTAAA 
GAAGACCCTG AAGGCTTGGC TCTAAGATTG GCTGGGAAAG GAGCTGTTTC CGCTGCGCTA 
GAAGTGGCTG AAAGTGCAGG ATTATCAATA GATCTTAGAA GAGAATTACA AGGACGGCAG 
CTTGTAAAGC TTCTAACCAC TGATCCACTC AATGGTGGTG GTCCGGCGGA AGCATCTCGG 
TTTTTATCTT CACTTCAAGA CTCGGCTGAT GCTCTACCAG TGGTTATGGG TGCAATGCAA 
TTATTACCTG ACCTTCGGTC GAAACAGCTC CTGGTCCATT TCTTTCTCAA GCGAAGAGAT 
AGCAATCTGT CGGATTTGGA AGTCGCCCGG CTTAATTCTT GGGCTTTAGG TCTGAAAGTG 
TTAGCTGCAT TACCACTTCC TTGGCAACAG AGATGCTCTT CGCTTCATGA GCATCCCAAT 
TTAATATTTG AAGCTCTGCT AATGAGAAAA CAATTACAAT ACGCCTCACT GATACTCAAA 
GAGTTCCCAG CATTAAGAGA TAACAATGTT ATCATGGCCT ATGCAGCAAA AGCTATTTCT 
GTGACAATTA TCCCACCACC AAGAGAACCT CGAATAACTG TGTCTGCGTC AAGGTTAAGA 
CAGAAATCAA GAGCAGGGCC AGCAGTAAAA GCATCCTTCA CTAGTAGCTT AAGCAATTTT 
CAGAGAGAGG CTCGAAGGGC ATTCTCATGG GCTCCACGTA ATGCTGAAAA CCGGACGACG 
TCAAAGGATG TTTATCGCAA AAGAAAGAAT TCCGGACTGG GGGCTTCTGA GAGAGCTGCA 
TGGGAGGCGA TGACAGGTAT TCAAGAGGAC CAGGGATCAT CTTATTCGGC AGATGGGCAA 
GATAGGCTAC CTTCTGTTTC TATTGCTGAA GAATGGATGC TAACTGGCGA CAAAACCAAA 
GATGAAGGTG TTCGTGCATC TCACAAGTAT GAAAGCACCC CCGATATTAT TCTCTTTAAG 
GCTCTACTAT CACTTTGTTC AGATGAGCTA GTATCAGCAA GAAGTGCCAT GGACCTATGC 
ATCAGTCAAA TGAAAAACGT ATTGAGCTCC AAACAGTTGT CAGAGGGTGC GTCTGTTGAA 
ACAATTGGCC GAGCATATCA TGCAACGGAG GCATTTGTGC AGGGTTTGTC GTATGCAAAA 
TCATTGCTGA GAAAGCTTCT AGGTACCACT GAATCGACCA ATAACAATGG TGAAAGAAGT 
AGAGATGTTG ATGATATATC TTCTGATGCT GGCAGCTCCA GTGTTGGCAG TCAGTCAACG 
GATGAACCAT CGGATGTTCT CTCACTTACA GAAATCTGGT TGGGGCGCGC AGAGTTGCTG 
CAGAGCCTCT TAGGGTCTGG AATTTCTACT TCTCTTGATG ACATTGCTGA TCAATTGTCA 
TCCGAATGTC TACGAGACAG ATTAATATCT GATGAACGAT ATAGTATGGC GGTGTATATG 
TGCAAGAAAT GCAAGATTGA TGTTTTCCCC GTGTGGAAAG CATGGGGTCT TGCTTTGTTA 
CGGATGGAGC GCTATGCTCA AGCTCGAGTC AAATTCAAGC AAGCCTTTCA ATTAAAGGGG 
GAAGACATTC CTGATGTCAT TCAGGAGATA ATAAATACAA TAGAAGGAGG TCCGCCTGTG 
GATGTGTCAA TTGTACGTTC CATGTATGAC CATTTGGCGA AAAGCGCACC TACAATTTTG 
GACGATTCTT TATCAGCAGA CTCATACCTA AATGTTCTGC ATATGCCATC CACTTTCCCT 
CGTTCAGAGA GGTCGCGGAG ATCTCTGGAA TCGGAAAAGA ATAGTTCTGT ACCTGGTTCA 
GACTTTGAAG ATGGGCCCCG AAGCAACTTG GATACTACAC GCTATTCTGA GTGCACCAAC 
TACTTGCAGG AACATGCTCG CCAAAACCTG CTTGGGTTTA TGTTCCGCCA TGGTCACTTT 
AAAGATGCAT GCATGTTATT CTTTCCGCAA AGTGGTCTTC CCCCTCCTTT GCAAACTTCA 
TCTGTGGGCG CAGTAAGCAC ATCTTCATCA CCTCAACGAA CTGATCCCTT GGCAACTGAA 
TATGGGACCA TTGAAAGTTT GTGCGAGTTC TGTGTTGGTT ATGGAGCTAT CTCATCCCTT 
GAGGAAGTAA TTACAGAAAG ACTTGAATCC GCAAAGAATC AAGATCAAGC CATAAATCAG 
TACATAGCTG GAGCTCTTAC TCGTATCTGT GCTTTCTTTG AGATCAACCG GCATTTCAAT 
TACCTATACA AGTTTCTGGT ACTCAAGAAG GATTATGTCA CCTCTGGGTA TTGTTGTATT 
CAGCTTTTTA TGAATTCTAC AACTCAGGAG GATGCTGTAA GGCATCTTGA GCATGCAAAG 
GCACACTTTG AAGAAGCATT GACAGCGCGT CATAGAGGTT CAGACTCAAA AAAACTTGTT 
ACAAAGGGTG TTAGAGGAAA AAGTGCCGCA GAGAAGCTGA GTGAAGAAAC TCTTGTTAAG 
TTGTCCTCAC GGGTGAAAAT GCAGATTGAT GTGGTGAAGT CCTTCAGTGA CTCTGAAGGA 
GCACCATGGA AGCATTCCTT GTTTGGAAAT CCAAATGATT CAGAGACATC CAGGAGAAGA 
TGTGAAATAG TGGAGACTCT TGTTGAGAAA AATTTCGACT TAGCTTATTC TGTTATATAT 
GAATTCAAGC TCTCAGCTGT TGATATATAT GCTGGTGTTG CTACGTCACT AGCTGATAGG 
AAGAAAGGCA GTCAGTTGAC AGAACTTTTC AAAAACATTA AGGGAACAAT CCAGGATGAT 
GACTGGGATC AGGTCCTGGG TGCTGCCATC AATATATATG CCAACAAGCA CAAAGAGCGC 
CCTGACCGTC TCATCGACAT GTTAACAAGC AGCCATCGAA AGGTGCTGGC TTGTGTGGTA 
TGTGGCCGCC TGAAAAGCGC ATTCCAGATT GCATCTAAAA GCGGAAGCGT GGCTGATGTT 
CAATATGTAG CTCATCAAGC CTTACATGCC AATTCGCACA CAGTACTCGA TATGTGCAAG 
CAATGGCTAG CTAAATACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324903.1
CDS	223..7686
Translation	MDKETEILSRLAANHLHLAQFEPLKATLLALRVRNPDLALTILQTIVSNAGRFDNVLWSRSCPSPSLLSFLSTIELLRFENPTSPWGFDSETLSLRADFLLMVQVLIDRVTERIKEDEESEDENSGLGNCLRVLQGVLELGVERLKFVVDTSSSEGSNKIEEDAVVSLRSIVLDYSDVFDALCCNIQRQLAGCESYGTCLVEEVQGEEQRKEMNEATCIGSPELDNINVFALIQRNVQLAQLDAMKTKLDEGDERGAADRIRYLHLDYGVEKENYHAVLKALLSRVMEKKDEYGDSWHMVRQNLLFMYKEALSSNCGDLVQMIQGIQDDMLLPHSQLHLSLDNEQIPLPLECFRRYLVDLKTERNIEDKSSPMSRAINSCLRDMYHYARISGSHVLECVMCAALSSVKKEKLQEANDVLTLFPRLRPLVASMGWDLLPGKTATRRKLMRLLWTSDSQALRLEESSLYGNQTDEKSCVEHLCDTLCYQLELASFAACVNSGKSWTPKASFLMHGNVSSAHDDAEVDPFVENLVLERLSAQSPLRVLFDVVPGIKFQDAISLISMQPIASTAEAWKRIEDIELMHMRYALEAIVLALGAMEEAMKDETDASHRVVFYHLKDLTNHLEAIKNVPRKIMMVNIVISLLHIDDIRLSSTQSASSACFSEKSNTPGLDPGDLGTEGEKEIVISFTKQLLDVLRRNLPSHPIEQECQLDGNYSTDGRQALEWRVSMAKRFIEDCEWRLSVMQHLLPLSERQWGLKEVLSILRAAPEKLLNLCMQRAKYDIGEEAVNRFALSAEDKATLELAEWVDNAFKGTLVEDVMSRTAEGAAAVQDLDFHSLGSQLSPLAMILLCIDAAASSAKSPTISKQLLDKSQVMLSEIYPGGAPKVGFTYWDQVHEVAIISVLRRILKRLQEFLEQDDPQILQASFSGDTIISSCTESHRQGQKDRALAMLHQMIEDAHRGKRQFLSGKLHNLARALADEKPEVDVLKGDGSDMAVEKDGVLGLGLKYTKQSPGSANRAVDGNPVSHETEDKGKKSFGPLSNKTSTYLSQFILYTAAIGDIVDGTDTTHDFNFFSLVYEWPKDLLTRLVFDRSSTDAAAKVAEVMSADFVHEVISACVPPVYPPRSGHGWACIPVIPTTPCSHSEGKVLSPSIEAKPNCYVRSSATPGVPLYPLQLDVIRHLVKISPVRAVLACVFGGSILYNGSDSIISSSLNDEFPSSPDADRLFYEFSLDQSERYPTLNRWIQMQTNLHRVSEFVVTPKQKPDDTRIKPDERTGIKRLLEHDSDSESDTEETFSKNNIQPALTDGSARDGGSFENGVCRTDPTVFLSFDWENEVPYEKAVNRLIDEGKLMDALALSDRFLRNGASDWLLQLLIKSREENPSTSGRSQGYGGQSNSWQYCLRLKDKQLAATLALKYMHTWELEAALNVLTMCSCHLLESDPISLEVLHRRQALQKYSHILSADDRHNSWQEVEAECKEDPEGLALRLAGKGAVSAALEVAESAGLSIDLRRELQGRQLVKLLTTDPLNGGGPAEASRFLSSLQDSADALPVVMGAMQLLPDLRSKQLLVHFFLKRRDSNLSDLEVARLNSWALGLKVLAALPLPWQQRCSSLHEHPNLIFEALLMRKQLQYASLILKEFPALRDNNVIMAYAAKAISVTIIPPPREPRITVSASRLRQKSRAGPAVKASFTSSLSNFQREARRAFSWAPRNAENRTTSKDVYRKRKNSGLGASERAAWEAMTGIQEDQGSSYSADGQDRLPSVSIAEEWMLTGDKTKDEGVRASHKYESTPDIILFKALLSLCSDELVSARSAMDLCISQMKNVLSSKQLSEGASVETIGRAYHATEAFVQGLSYAKSLLRKLLGTTESTNNNGERSRDVDDISSDAGSSSVGSQSTDEPSDVLSLTEIWLGRAELLQSLLGSGISTSLDDIADQLSSECLRDRLISDERYSMAVYMCKKCKIDVFPVWKAWGLALLRMERYAQARVKFKQAFQLKGEDIPDVIQEIINTIEGGPPVDVSIVRSMYDHLAKSAPTILDDSLSADSYLNVLHMPSTFPRSERSRRSLESEKNSSVPGSDFEDGPRSNLDTTRYSECTNYLQEHARQNLLGFMFRHGHFKDACMLFFPQSGLPPPLQTSSVGAVSTSSSPQRTDPLATEYGTIESLCEFCVGYGAISSLEEVITERLESAKNQDQAINQYIAGALTRICAFFEINRHFNYLYKFLVLKKDYVTSGYCCIQLFMNSTTQEDAVRHLEHAKAHFEEALTARHRGSDSKKLVTKGVRGKSAAEKLSEETLVKLSSRVKMQIDVVKSFSDSEGAPWKHSLFGNPNDSETSRRRCEIVETLVEKNFDLAYSVIYEFKLSAVDIYAGVATSLADRKKGSQLTELFKNIKGTIQDDDWDQVLGAAINIYANKHKERPDRLIDMLTSSHRKVLACVVCGRLKSAFQIASKSGSVADVQYVAHQALHANSHTVLDMCKQWLAKYM

Target ORF information:

RefSeq Version	NM_001336017.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336017.1

ORF Insert Sequence:

ATGGATAAAG AGACGGAGAT TCTCTCCCGT CTCGCGGCGA ACCACCTTCA TCTGGCTCAA 
TTCGAGCCAT TGAAGGCTAC GTTACTCGCT CTCAGGGTTC GTAACCCTGA CCTCGCACTC 
ACCATTCTCC AAACCATCGT CTCCAACGCT GGAAGATTCG ATAATGTCCT CTGGTCACGC 
TCTTGTCCTT CCCCGTCTCT TCTCTCGTTC CTCTCCACGA TTGAGCTTCT GAGATTCGAA 
AATCCTACTT CTCCTTGGGG ATTTGATTCA GAAACTCTAA GTTTGCGTGC CGATTTCTTG 
TTGATGGTTC AGGTTTTGAT CGATAGAGTT ACAGAGAGGA TTAAGGAAGA TGAGGAGAGT 
GAGGATGAAA ATTCTGGATT AGGGAATTGT TTAAGGGTGT TGCAAGGTGT TTTGGAGTTA 
GGTGTTGAGA GGTTGAAGTT TGTTGTTGAT ACTAGTAGTA GTGAAGGAAG TAATAAGATT 
GAGGAAGATG CAGTTGTGTC TTTGAGGAGT ATAGTATTGG ATTACTCTGA TGTTTTCGAT 
GCTTTATGTT GTAATATTCA GAGGCAACTT GCGGGTTGCG AGAGTTACGG TACATGTTTG 
GTTGAGGAAG TTCAGGGAGA AGAACAGAGA AAGGAGATGA ATGAGGCCAC ATGTATTGGT 
TCTCCGGAGC TGGATAACAT CAATGTGTTT GCTTTGATAC AGAGGAATGT TCAGTTAGCA 
CAGTTGGATG CTATGAAAAC AAAGTTGGAT GAAGGTGATG AGCGCGGGGC AGCTGATCGC 
ATTCGTTATC TTCACCTTGA TTATGGAGTA GAGAAAGAGA ACTATCATGC TGTTCTAAAA 
GCTCTCCTTT CAAGAGTTAT GGAGAAAAAG GATGAATATG GTGATTCCTG GCACATGGTG 
CGCCAGAACT TGCTGTTTAT GTATAAAGAA GCTCTCTCAT CGAATTGTGG AGATCTTGTT 
CAGATGATCC AGGGTATTCA AGATGATATG CTCCTCCCAC ATAGCCAACT ACATTTATCT 
CTCGACAATG AACAAATTCC ACTCCCTCTT GAATGTTTCC GGCGATATCT TGTAGACTTG 
AAAACTGAGA GAAATATAGA GGACAAAAGT TCTCCTATGA GCAGGGCAAT TAATTCTTGT 
CTCAGAGATA TGTATCATTA TGCTCGTATT TCTGGATCAC ATGTTCTTGA GTGTGTGATG 
TGTGCTGCTT TGTCTTCTGT AAAGAAAGAG AAGCTTCAGG AGGCTAATGA TGTTCTTACT 
TTGTTTCCCC GACTTCGCCC TTTAGTAGCC TCCATGGGTT GGGATCTATT GCCGGGCAAA 
ACTGCAACCC GTAGAAAATT GATGCGGCTA CTTTGGACTA GTGACTCGCA AGCACTTCGG 
CTAGAAGAAT CTTCTCTTTA TGGAAACCAG ACAGATGAAA AATCTTGTGT GGAACACCTC 
TGCGATACGT TATGTTATCA GCTGGAACTT GCATCTTTCG CTGCTTGTGT CAATTCTGGT 
AAATCATGGA CTCCAAAGGC ATCTTTCTTG ATGCATGGTA ATGTGTCATC CGCGCATGAT 
GATGCGGAGG TGGATCCTTT TGTTGAAAAT CTTGTATTGG AAAGGCTTTC AGCGCAAAGT 
CCACTTCGGG TATTGTTTGA CGTTGTTCCG GGCATAAAAT TCCAAGATGC TATTTCACTG 
ATTAGTATGC AACCTATTGC TTCAACTGCA GAAGCCTGGA AGAGGATAGA AGATATTGAA 
CTGATGCATA TGCGTTATGC TCTGGAGGCA ATCGTTTTAG CACTAGGTGC AATGGAAGAG 
GCTATGAAGG ATGAGACAGA TGCTAGTCAT CGAGTAGTAT TTTACCATTT AAAAGACCTC 
ACTAACCATT TGGAGGCCAT TAAAAATGTT CCACGCAAGA TAATGATGGT GAACATAGTT 
ATTTCACTCT TACATATTGA TGATATCCGT CTCAGTTCTA CGCAAAGTGC CTCCTCGGCA 
TGTTTTTCTG AAAAAAGTAA CACACCTGGT TTGGATCCTG GCGATCTTGG TACAGAAGGG 
GAAAAGGAAA TTGTTATTTC TTTCACAAAA CAGCTACTCG ATGTTTTACG CCGCAATCTT 
CCATCACATC CAATTGAACA AGAGTGTCAG CTGGATGGTA ATTACAGTAC TGATGGAAGA 
CAGGCTTTAG AATGGAGAGT ATCCATGGCT AAGCGTTTCA TTGAAGATTG TGAATGGCGA 
TTATCTGTTA TGCAGCATCT TCTGCCACTT TCTGAACGCC AGTGGGGTTT AAAGGAGGTT 
TTGAGTATTC TAAGGGCAGC CCCTGAAAAA CTGCTTAATC TCTGTATGCA AAGAGCTAAG 
TATGACATTG GAGAAGAGGC AGTTAATCGG TTTGCGTTAT CAGCAGAGGA CAAAGCTACT 
CTTGAATTAG CTGAATGGGT TGATAATGCG TTCAAAGGAA CACTGGTAGA AGATGTAATG 
TCTCGTACTG CTGAAGGAGC AGCTGCCGTG CAAGATTTAG ATTTTCATTC TTTAGGTTCT 
CAATTGAGTC CATTGGCTAT GATTCTACTG TGCATAGATG CGGCTGCTTC TTCTGCCAAG 
TCTCCAACAA TTTCCAAACA ACTTTTGGAT AAGTCTCAAG TTATGTTATC GGAAATTTAC 
CCTGGAGGAG CTCCGAAGGT GGGGTTTACT TACTGGGATC AGGTCCACGA AGTTGCAATA 
ATTTCTGTAT TGCGAAGGAT CTTAAAGCGT CTGCAGGAAT TCCTTGAACA GGATGACCCT 
CAAATTCTTC AAGCCAGTTT TAGTGGAGAT ACCATAATTT CATCTTGCAC GGAATCTCAT 
AGACAGGGAC AAAAAGATCG TGCTCTTGCA ATGCTACATC AAATGATTGA GGATGCTCAT 
AGGGGCAAGC GTCAGTTCCT GAGTGGTAAG CTTCATAACT TAGCGAGAGC ACTCGCTGAT 
GAAAAACCAG AAGTTGACGT ACTCAAAGGG GACGGATCAG ACATGGCCGT TGAGAAGGAT 
GGAGTTCTTG GTCTTGGGCT AAAATATACA AAGCAAAGTC CTGGTTCAGC AAATAGAGCC 
GTGGATGGAA ATCCTGTTTC ACATGAAACA GAAGACAAGG GAAAGAAGTC ATTTGGCCCA 
TTAAGCAACA AAACCTCTAC TTATCTATCT CAGTTTATCC TCTATACTGC TGCTATTGGT 
GATATAGTAG ATGGAACTGA CACAACCCAT GATTTCAACT TTTTCTCTCT TGTTTATGAA 
TGGCCTAAAG ACCTATTGAC GCGTCTGGTT TTTGATCGAA GTAGCACAGA TGCAGCTGCA 
AAAGTTGCTG AGGTTATGTC TGCTGATTTT GTTCATGAAG TGATATCAGC ATGTGTTCCC 
CCAGTTTATC CCCCACGTTC TGGTCATGGG TGGGCTTGTA TTCCCGTCAT TCCAACCACT 
CCATGTTCCC ACTCAGAGGG TAAAGTGCTC TCTCCTTCAA TAGAGGCTAA ACCCAACTGT 
TATGTCCGTT CCTCAGCAAC ACCTGGTGTC CCTCTGTATC CTCTTCAGTT GGATGTTATC 
AGGCATTTGG TAAAAATTTC ACCAGTACGA GCAGTTTTAG CTTGCGTCTT TGGTGGGAGC 
ATATTGTACA ATGGCAGTGA TTCTATCATA TCTAGCTCCT TGAACGATGA GTTTCCAAGT 
TCTCCTGATG CAGACAGATT GTTTTATGAA TTTTCTCTTG ATCAGTCTGA GAGGTATCCC 
ACTTTAAACC GATGGATACA GATGCAGACT AATCTGCATC GAGTTTCGGA ATTTGTTGTG 
ACACCGAAGC AAAAACCTGA TGACACACGG ATTAAGCCTG ATGAAAGAAC TGGGATCAAG 
AGACTTCTTG AACATGATAG TGACTCAGAG TCAGATACAG AAGAAACATT TTCTAAAAAT 
AACATTCAAC CAGCATTGAC AGACGGCAGT GCTCGTGATG GTGGATCCTT TGAAAATGGA 
GTTTGTAGAA CTGATCCTAC CGTTTTCCTT TCTTTTGATT GGGAGAATGA AGTACCGTAT 
GAGAAAGCTG TAAATAGACT AATTGATGAA GGAAAACTAA TGGATGCTTT AGCACTTTCG 
GACCGCTTCT TGCGGAATGG GGCTTCTGAT TGGTTACTTC AGCTCCTAAT CAAAAGTAGA 
GAAGAAAATC CTTCAACATC AGGACGATCT CAGGGATATG GAGGCCAGAG CAACAGTTGG 
CAGTATTGCC TGCGGCTAAA GGACAAACAG CTGGCAGCAA CACTGGCTCT TAAGTATATG 
CATACGTGGG AGCTTGAGGC AGCTTTAAAT GTTCTTACAA TGTGTAGTTG TCATCTCTTA 
GAAAGCGATC CAATTAGTCT TGAGGTGTTG CATAGGAGAC AAGCTCTGCA GAAGTACAGC 
CACATACTTT CGGCAGATGA TCGCCATAAT AGCTGGCAAG AGGTAGAAGC CGAATGTAAA 
GAAGACCCTG AAGGCTTGGC TCTAAGATTG GCTGGGAAAG GAGCTGTTTC CGCTGCGCTA 
GAAGTGGCTG AAAGTGCAGG ATTATCAATA GATCTTAGAA GAGAATTACA AGGACGGCAG 
CTTGTAAAGC TTCTAACCAC TGATCCACTC AATGGTGGTG GTCCGGCGGA AGCATCTCGG 
TTTTTATCTT CACTTCAAGA CTCGGCTGAT GCTCTACCAG TGGTTATGGG TGCAATGCAA 
TTATTACCTG ACCTTCGGTC GAAACAGCTC CTGGTCCATT TCTTTCTCAA GCGAAGAGAT 
AGCAATCTGT CGGATTTGGA AGTCGCCCGG CTTAATTCTT GGGCTTTAGG TCTGAAAGTG 
TTAGCTGCAT TACCACTTCC TTGGCAACAG AGATGCTCTT CGCTTCATGA GCATCCCAAT 
TTAATATTTG AAGCTCTGCT AATGAGAAAA CAATTACAAT ACGCCTCACT GATACTCAAA 
GAGTTCCCAG CATTAAGAGA TAACAATGTT ATCATGGCCT ATGCAGCAAA AGCTATTTCT 
GTGACAATTA TCCCACCACC AAGAGAACCT CGAATAACTG TGTCTGCGTC AAGGTTAAGA 
CAGAAATCAA GAGCAGGGCC AGCAGTAAAA GCATCCTTCA CTAGTAGCTT AAGCAATTTT 
CAGAGAGAGG CTCGAAGGGC ATTCTCATGG GCTCCACGTA ATGCTGAAAA CCGGACGACG 
TCAAAGGATG TTTATCGCAA AAGAAAGAAT TCCGGACTGG GGGCTTCTGA GAGAGCTGCA 
TGGGAGGCGA TGACAGGTAT TCAAGAGGAC CAGGGATCAT CTTATTCGGC AGATGGGCAA 
GATAGGCTAC CTTCTGTTTC TATTGCTGAA GAATGGATGC TAACTGGCGA CAAAACCAAA 
GATGAAGGTG TTCGTGCATC TCACAAGTAT GAAAGCACCC CCGATATTAT TCTCTTTAAG 
GCTCTACTAT CACTTTGTTC AGATGAGCTA GTATCAGCAA GAAGTGCCAT GGACCTATGC 
ATCAGTCAAA TGAAAAACGT ATTGAGCTCC AAACAGTTGT CAGAGGGTGC GTCTGTTGAA 
ACAATTGGCC GAGCATATCA TGCAACGGAG GCATTTGTGC AGGGTTTGTC GTATGCAAAA 
TCATTGCTGA GAAAGCTTCT AGGTACCACT GAATCGACCA ATAACAATGG TGAAAGAAGT 
AGAGATGTTG ATGATATATC TTCTGATGCT GGCAGCTCCA GTGTTGGCAG TCAGTCAACG 
GATGAACCAT CGGATGTTCT CTCACTTACA GAAATCTGGT TGGGGCGCGC AGAGTTGCTG 
CAGAGCCTCT TAGGGTCTGG AATTTCTACT TCTCTTGATG ACATTGCTGA TCAATTGTCA 
TCCGAATGTC TACGAGACAG ATTAATATCT GATGAACGAT ATAGTATGGC GGTGTATATG 
TGCAAGAAAT GCAAGATTGA TGTTTTCCCC GTGTGGAAAG CATGGGGTCT TGCTTTGTTA 
CGGATGGAGC GCTATGCTCA AGCTCGAGTC AAATTCAAGC AAGCCTTTCA ATTAAAGGGG 
GAAGACATTC CTGATGTCAT TCAGGAGATA ATAAATACAA TAGAAGGAGG TCCGCCTGTG 
GATGTGTCAA TTGTACGTTC CATGTATGAC CATTTGGCGA AAAGCGCACC TACAATTTTG 
GACGATTCTT TATCAGCAGA CTCATACCTA AATGTTCTGC ATATGCCATC CACTTTCCCT 
CGTTCAGAGA GGTCGCGGAG ATCTCTGGAA TCGGAAAAGA ATAGTTCTGT ACCTGGTTCA 
GACTTTGAAG ATGGGCCCCG AAGCAACTTG GATACTACAC GCTATTCTGA GTGCACCAAC 
TACTTGCAGG AACATGCTCG CCAAAACCTG CTTGGGTTTA TGTTCCGCCA TGGTCACTTT 
AAAGATGCAT GCATGTTATT CTTTCCGCAA AGTGGTCTTC CCCCTCCTTT GCAAACTTCA 
TCTGTGGGCG CAGTAAGCAC ATCTTCATCA CCTCAACGAA CTGATCCCTT GGCAACTGAA 
TATGGGACCA TTGAAAGTTT GTGCGAGTTC TGTGTTGGTT ATGGAGCTAT CTCATCCCTT 
GAGGAAGTAA TTACAGAAAG ACTTGAATCC GCAAAGAATC AAGATCAAGC CATAAATCAG 
TACATAGCTG GAGCTCTTAC TCGTATCTGT GCTTTCTTTG AGATCAACCG GCATTTCAAT 
TACCTATACA AGTTTCTGGT ACTCAAGAAG GATTATGTCA CCTCTGGGTA TTGTTGTATT 
CAGCTTTTTA TGAATTCTAC AACTCAGGAG GATGCTGTAA GGCATCTTGA GCATGCAAAG 
GCACACTTTG AAGAAGCATT GACAGCGCGT CATAGAGGTT CAGACTCAAA AAAACTTGTT 
ACAAAGGGTG TTAGAGGAAA AAGTGCCGCA GAGAAGCTGA GTGAAGAAAC TCTTGTTAAG 
TTGTCCTCAC GGGTGAAAAT GCAGATTGAT GTGGTGAAGT CCTTCAGTGA CTCTGAAGGA 
GCACCATGGA AGCATTCCTT GTTTGGAAAT CCAAATGATT CAGAGACATC CAGGAGAAGA 
TGTGAAATAG TGGAGACTCT TGTTGAGAAA AATTTCGACT TAGCTTATTC TGTTATATAT 
GAATTCAAGC TCTCAGCTGT TGATATATAT GCTGGTGTTG CTACGTCACT AGCTGATAGG 
AAGAAAGGCA GTCAGTTGAC AGAACTTTTC AAAAACATTA AGGGAACAAT CCAGGATGAT 
GACTGGGATC AGGTCCTGGG TGCTGCCATC AATATATATG CCAACAAGCA CAAAGAGCGC 
CCTGACCGTC TCATCGACAT GTTAACAAGC AGCCATCGAA AGGTGCTGGC TTGTGTGGTA 
TGTGGCCGCC TGAAAAGCGC ATTCCAGATT GCATCTAAAA GCGGAAGCGT GGCTGATGTT 
CAATATGTAG CTCATCAAGC CTTACATGCC AATTCGCACA CAGTACTCGA TATGTGCAAG 
CAATGGCTAG CTAAATACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT2G25730 ( NM_001336018.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324902.1 Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), partial mRNA.

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CloneID	OAb36030
Clone ID Related Accession (Same CDS sequence)	NM_001336018.1
Accession Version	NM_001336018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	zinc finger FYVE domain protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). COMPLETENESS: incomplete on the 5' end.

ATGGATAAAG AGACGGAGAT TCTCTCCCGT CTCGCGGCGA ACCACCTTCA TCTGGCTCAA 
TTCGAGCCAT TGAAGGCTAC GTTACTCGCT CTCAGGGTTC GTAACCCTGA CCTCGCACTC 
ACCATTCTCC AAACCATCGT CTCCAACGCT GGAAGATTCG ATAATGTCCT CTGGTCACGC 
TCTTGTCCTT CCCCGTCTCT TCTCTCGTTC CTCTCCACGA TTGAGCTTCT GAGATTCGAA 
AATCCTACTT CTCCTTGGGG ATTTGATTCA GAAACTCTAA GTTTGCGTGC CGATTTCTTG 
TTGATGGTTC AGGTTTTGAT CGATAGAGTT ACAGAGAGGA TTAAGGAAGA TGAGGAGAGT 
GAGGATGAAA ATTCTGGATT AGGGAATTGT TTAAGGGTGT TGCAAGGTGT TTTGGAGTTA 
GGTGTTGAGA GGTTGAAGTT TGTTGTTGAT ACTAGTAGTA GTGAAGGAAG TAATAAGATT 
GAGGAAGATG CAGTTGTGTC TTTGAGGAGT ATAGTATTGG ATTACTCTGA TGTTTTCGAT 
GCTTTATGTT GTAATATTCA GAGGCAACTT GCGGGTTGCG AGAGTTACGG TACATGTTTG 
GTTGAGGAAG TTCAGGGAGA AGAACAGAGA AAGGAGATGA ATGAGGCCAC ATGTATTGGT 
TCTCCGGAGC TGGATAACAT CAATGTGTTT GCTTTGATAC AGAGGAATGT TCAGTTAGCA 
CAGTTGGATG CTATGAAAAC AAAGTTGGAT GAAGGTGATG AGCGCGGGGC AGCTGATCGC 
ATTCGTTATC TTCACCTTGA TTATGGAGTA GAGAAAGAGA ACTATCATGC TGTTCTAAAA 
GCTCTCCTTT CAAGAGTTAT GGAGAAAAAG GATGAATATG GTGATTCCTG GCACATGGTG 
CGCCAGAACT TGCTGTTTAT GTATAAAGAA GCTCTCTCAT CGAATTGTGG AGATCTTGTT 
CAGATGATCC AGGGTATTCA AGATGATATG CTCCTCCCAC ATAGCCAACT ACATTTATCT 
CTCGACAATG AACAAATTCC ACTCCCTCTT GAATGTTTCC GGCGATATCT TGTAGACTTG 
AAAACTGAGA GAAATATAGA GGACAAAAGT TCTCCTATGA GCAGGGCAAT TAATTCTTGT 
CTCAGAGATA TGTATCATTA TGCTCGTATT TCTGGATCAC ATGTTCTTGA GTGTGTGATG 
TGTGCTGCTT TGTCTTCTGT AAAGAAAGAG AAGCTTCAGG AGGCTAATGA TGTTCTTACT 
TTGTTTCCCC GACTTCGCCC TTTAGTAGCC TCCATGGGTT GGGATCTATT GCCGGGCAAA 
ACTGCAACCC GTAGAAAATT GATGCGGCTA CTTTGGACTA GTGACTCGCA AGCACTTCGG 
CTAGAAGAAT CTTCTCTTTA TGGAAACCAG ACAGATGAAC TGGAACTTGC ATCTTTCGCT 
GCTTGTGTCA ATTCTGGTAA ATCATGGACT CCAAAGGCAT CTTTCTTGAT GCATGGTAAT 
GTGTCATCCG CGCATGATGA TGCGGAGGTG GATCCTTTTG TTGAAAATCT TGTATTGGAA 
AGGCTTTCAG CGCAAAGTCC ACTTCGGGTA TTGTTTGACG TTGTTCCGGG CATAAAATTC 
CAAGATGCTA TTTCACTGAT TAGTATGCAA CCTATTGCTT CAACTGCAGA AGCCTGGAAG 
AGGATAGAAG ATATTGAACT GATGCATATG CGTTATGCTC TGGAGGCAAT CGTTTTAGCA 
CTAGGTGCAA TGGAAGAGGC TATGAAGGAT GAGACAGATG CTAGTCATCG AGTAGTATTT 
TACCATTTAA AAGACCTCAC TAACCATTTG GAGGCCATTA AAAATGTTCC ACGCAAGATA 
ATGATGGTGA ACATAGTTAT TTCACTCTTA CATATTGATG ATATCCGTCT CAGTTCTACG 
CAAAGTGCCT CCTCGGCATG TTTTTCTGAA AAAAGTAACA CACCTGGTTT GGATCCTGGC 
GATCTTGGTA CAGAAGGGGA AAAGGAAATT GTTATTTCTT TCACAAAACA GCTACTCGAT 
GTTTTACGCC GCAATCTTCC ATCACATCCA ATTGAACAAG AGTGTCAGCT GGATGGTAAT 
TACAGTACTG ATGGAAGACA GGCTTTAGAA TGGAGAGTAT CCATGGCTAA GCGTTTCATT 
GAAGATTGTG AATGGCGATT ATCTGTTATG CAGCATCTTC TGCCACTTTC TGAACGCCAG 
TGGGGTTTAA AGGAGGTTTT GAGTATTCTA AGGGCAGCCC CTGAAAAACT GCTTAATCTC 
TGTATGCAAA GAGCTAAGTA TGACATTGGA GAAGAGGCAG TTAATCGGTT TGCGTTATCA 
GCAGAGGACA AAGCTACTCT TGAATTAGCT GAATGGGTTG ATAATGCGTT CAAAGGAACA 
CTGGTAGAAG ATGTAATGTC TCGTACTGCT GAAGGAGCAG CTGCCGTGCA AGATTTAGAT 
TTTCATTCTT TAGGTTCTCA ATTGAGTCCA TTGGCTATGA TTCTACTGTG CATAGATGCG 
GCTGCTTCTT CTGCCAAGTC TCCAACAATT TCCAAACAAC TTTTGGATAA GTCTCAAGTT 
ATGTTATCGG AAATTTACCC TGGAGGAGCT CCGAAGGTGG GGTTTACTTA CTGGGATCAG 
GTCCACGAAG TTGCAATAAT TTCTGTATTG CGAAGGATCT TAAAGCGTCT GCAGGAATTC 
CTTGAACAGG ATGACCCTCA AATTCTTCAA GCCAGTTTTA GTGGAGATAC CATAATTTCA 
TCTTGCACGG AATCTCATAG ACAGGGACAA AAAGATCGTG CTCTTGCAAT GCTACATCAA 
ATGATTGAGG ATGCTCATAG GGGCAAGCGT CAGTTCCTGA GTGGTAAGCT TCATAACTTA 
GCGAGAGCAC TCGCTGATGA AAAACCAGAA GTTGACGTAC TCAAAGGGGA CGGATCAGAC 
ATGGCCGTTG AGAAGGATGG AGTTCTTGGT CTTGGGCTAA AATATACAAA GCAAAGTCCT 
GGTTCAGCAA ATAGAGCCGT GGATGGAAAT CCTGTTTCAC ATGAAACAGA AGACAAGGGA 
AAGAAGTCAT TTGGCCCATT AAGCAACAAA ACCTCTACTT ATCTATCTCA GTTTATCCTC 
TATACTGCTG CTATTGGTGA TATAGTAGAT GGAACTGACA CAACCCATGA TTTCAACTTT 
TTCTCTCTTG TTTATGAATG GCCTAAAGAC CTATTGACGC GTCTGGTTTT TGATCGAAGT 
AGCACAGATG CAGCTGCAAA AGTTGCTGAG GTTATGTCTG CTGATTTTGT TCATGAAGTG 
ATATCAGCAT GTGTTCCCCC AGTTTATCCC CCACGTTCTG GTCATGGGTG GGCTTGTATT 
CCCGTCATTC CAACCACTCC ATGTTCCCAC TCAGAGGGTA AAGTGCTCTC TCCTTCAATA 
GAGGCTAAAC CCAACTGTTA TGTCCGTTCC TCAGCAACAC CTGGTGTCCC TCTGTATCCT 
CTTCAGTTGG ATGTTATCAG GCATTTGGTA AAAATTTCAC CAGTACGAGC AGTTTTAGCT 
TGCGTCTTTG GTGGGAGCAT ATTGTACAAT GGCAGTGATT CTATCATATC TAGCTCCTTG 
AACGATGAGT TTCCAAGTTC TCCTGATGCA GACAGATTGT TTTATGAATT TTCTCTTGAT 
CAGTCTGAGA GGTATCCCAC TTTAAACCGA TGGATACAGA TGCAGACTAA TCTGCATCGA 
GTTTCGGAAT TTGTTGTGAC ACCGAAGCAA AAACCTGATG ACACACGGAT TAAGCCTGAT 
GAAAGAACTG GGATCAAGAG ACTTCTTGAA CATGATAGTG ACTCAGAGTC AGATACAGAA 
GAAACATTTT CTAAAAATAA CATTCAACCA GCATTGACAG ACGGCAGTGC TCGTGATGGT 
GGATCCTTTG AAAATGGAGT TTGTAGAACT GATCCTACCG TTTTCCTTTC TTTTGATTGG 
GAGAATGAAG TACCGTATGA GAAAGCTGTA AATAGACTAA TTGATGAAGG AAAACTAATG 
GATGCTTTAG CACTTTCGGA CCGCTTCTTG CGGAATGGGG CTTCTGATTG GTTACTTCAG 
CTCCTAATCA AAAGTAGAGA AGAAAATCCT TCAACATCAG GACGATCTCA GGGATATGGA 
GGCCAGAGCA ACAGTTGGCA GTATTGCCTG CGGCTAAAGG ACAAACAGCT GGCAGCAACA 
CTGGCTCTTA AGTATATGCA TACGTGGGAG CTTGAGGCAG CTTTAAATGT TCTTACAATG 
TGTAGTTGTC ATCTCTTAGA AAGCGATCCA ATTAGTCTTG AGGTGTTGCA TAGGAGACAA 
GCTCTGCAGA AGTACAGCCA CATACTTTCG GCAGATGATC GCCATAATAG CTGGCAAGAG 
GTAGAAGCCG AATGTAAAGA AGACCCTGAA GGCTTGGCTC TAAGATTGGC TGGGAAAGGA 
GCTGTTTCCG CTGCGCTAGA AGTGGCTGAA AGTGCAGGAT TATCAATAGA TCTTAGAAGA 
GAATTACAAG GACGGCAGCT TGTAAAGCTT CTAACCACTG ATCCACTCAA TGGTGGTGGT 
CCGGCGGAAG CATCTCGGTT TTTATCTTCA CTTCAAGACT CGGCTGATGC TCTACCAGTG 
GTTATGGGTG CAATGCAATT ATTACCTGAC CTTCGGTCGA AACAGCTCCT GGTCCATTTC 
TTTCTCAAGC GAAGAGATAG CAATCTGTCG GATTTGGAAG TCGCCCGGCT TAATTCTTGG 
GCTTTAGGTC TGAAAGTGTT AGCTGCATTA CCACTTCCTT GGCAACAGAG ATGCTCTTCG 
CTTCATGAGC ATCCCAATTT AATATTTGAA GCTCTGCTAA TGAGAAAACA ATTACAATAC 
GCCTCACTGA TACTCAAAGA GTTCCCAGCA TTAAGAGATA ACAATGTTAT CATGGCCTAT 
GCAGCAAAAG CTATTTCTGT GACAATTATC CCACCACCAA GAGAACCTCG AATAACTGTG 
TCTGCGTCAA GGTTAAGACA GAAATCAAGA GCAGGGCCAG CAGTAAAAGC ATCCTTCACT 
AGTAGCTTAA GCAATTTTCA GAGAGAGGCT CGAAGGGCAT TCTCATGGGC TCCACGTAAT 
GCTGAAAACC GGACGACGTC AAAGGATGTT TATCGCAAAA GAAAGAATTC CGGACTGGGG 
GCTTCTGAGA GAGCTGCATG GGAGGCGATG ACAGGTATTC AAGAGGACCA GGGATCATCT 
TATTCGGCAG ATGGGCAAGA TAGGCTACCT TCTGTTTCTA TTGCTGAAGA ATGGATGCTA 
ACTGGCGACA AAACCAAAGA TGAAGGTGTT CGTGCATCTC ACAAGTATGA AAGCACCCCC 
GATATTATTC TCTTTAAGGC TCTACTATCA CTTTGTTCAG ATGAGCTAGT ATCAGCAAGA 
AGTGCCATGG ACCTATGCAT CAGTCAAATG AAAAACGTAT TGAGCTCCAA ACAGTTGTCA 
GAGGGTGCGT CTGTTGAAAC AATTGGCCGA GCATATCATG CAACGGAGGC ATTTGTGCAG 
GGTTTGTCGT ATGCAAAATC ATTGCTGAGA AAGCTTCTAG GTACCACTGA ATCGACCAAT 
AACAATGGTG AAAGAAGTAG AGATGTTGAT GATATATCTT CTGATGCTGG CAGCTCCAGT 
GTTGGCAGTC AGTCAACGGA TGAACCATCG GATGTTCTCT CACTTACAGA AATCTGGTTG 
GGGCGCGCAG AGTTGCTGCA GAGCCTCTTA GGGTCTGGAA TTTCTACTTC TCTTGATGAC 
ATTGCTGATC AATTGTCATC CGAATGTCTA CGAGACAGAT TAATATCTGA TGAACGATAT 
AGTATGGCGG TGTATATGTG CAAGAAATGC AAGATTGATG TTTTCCCCGT GTGGAAAGCA 
TGGGGTCTTG CTTTGTTACG GATGGAGCGC TATGCTCAAG CTCGAGTCAA ATTCAAGCAA 
GCCTTTCAAT TAAAGGGGGA AGACATTCCT GATGTCATTC AGGAGATAAT AAATACAATA 
GAAGGAGGTC CGCCTGTGGA TGTGTCAATT GTACGTTCCA TGTATGACCA TTTGGCGAAA 
AGCGCACCTA CAATTTTGGA CGATTCTTTA TCAGCAGACT CATACCTAAA TGTTCTGCAT 
ATGCCATCCA CTTTCCCTCG TTCAGAGAGG TCGCGGAGAT CTCTGGAATC GGAAAAGAAT 
AGTTCTGTAC CTGGTTCAGA CTTTGAAGAT GGGCCCCGAA GCAACTTGGA TACTACACGC 
TATTCTGAGT GCACCAACTA CTTGCAGGAA CATGCTCGCC AAAACCTGCT TGGGTTTATG 
TTCCGCCATG GTCACTTTAA AGATGCATGC ATGTTATTCT TTCCGCAAAG TGGTCTTCCC 
CCTCCTTTGC AAACTTCATC TGTGGGCGCA GTAAGCACAT CTTCATCACC TCAACGAACT 
GATCCCTTGG CAACTGAATA TGGGACCATT GAAAGTTTGT GCGAGTTCTG TGTTGGTTAT 
GGAGCTATCT CATCCCTTGA GGAAGTAATT ACAGAAAGAC TTGAATCCGC AAAGAATCAA 
GATCAAGCCA TAAATCAGTA CATAGCTGGA GCTCTTACTC GTATCTGTGC TTTCTTTGAG 
ATCAACCGGC ATTTCAATTA CCTATACAAG TTTCTGGTAC TCAAGAAGGA TTATGTCACC 
TCTGGGTATT GTTGTATTCA GCTTTTTATG AATTCTACAA CTCAGGAGGA TGCTGTAAGG 
CATCTTGAGC ATGCAAAGGC ACACTTTGAA GAAGCATTGA CAGCGCGTCA TAGAGGTTCA 
GACTCAAAAA AACTTGTTAC AAAGGGTGTT AGAGGAAAAA GTGCCGCAGA GAAGCTGAGT 
GAAGAAACTC TTGTTAAGTT GTCCTCACGG GTGAAAATGC AGATTGATGT GGTGAAGTCC 
TTCAGTGACT CTGAAGGAGC ACCATGGAAG CATTCCTTGT TTGGAAATCC AAATGATTCA 
GAGACATCCA GGAGAAGATG TGAAATAGTG GAGACTCTTG TTGAGAAAAA TTTCGACTTA 
GCTTATTCTG TTATATATGA ATTCAAGCTC TCAGCTGTTG ATATATATGC TGGTGTTGCT 
ACGTCACTAG CTGATAGGAA GAAAGGCAGT CAGTTGACAG AACTTTTCAA AAACATTAAG 
GGAACAATCC AGGATGATGA CTGGGATCAG GTCCTGGGTG CTGCCATCAA TATATATGCC 
AACAAGCACA AAGAGCGCCC TGACCGTCTC ATCGACATGT TAACAAGCAG CCATCGAAAG 
GTGCTGGCTT GTGTGGTATG TGGCCGCCTG AAAAGCGCAT TCCAGATTGC ATCTAAAAGC 
GGAAGCGTGG CTGATGTTCA ATATGTAGCT CATCAAGCCT TACATGCCAA TTCGCACACA 
GTACTCGATA TGTGCAAGCA ATGGCTAGCT AAATACATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324902.1
CDS	1..7422
Translation	MDKETEILSRLAANHLHLAQFEPLKATLLALRVRNPDLALTILQTIVSNAGRFDNVLWSRSCPSPSLLSFLSTIELLRFENPTSPWGFDSETLSLRADFLLMVQVLIDRVTERIKEDEESEDENSGLGNCLRVLQGVLELGVERLKFVVDTSSSEGSNKIEEDAVVSLRSIVLDYSDVFDALCCNIQRQLAGCESYGTCLVEEVQGEEQRKEMNEATCIGSPELDNINVFALIQRNVQLAQLDAMKTKLDEGDERGAADRIRYLHLDYGVEKENYHAVLKALLSRVMEKKDEYGDSWHMVRQNLLFMYKEALSSNCGDLVQMIQGIQDDMLLPHSQLHLSLDNEQIPLPLECFRRYLVDLKTERNIEDKSSPMSRAINSCLRDMYHYARISGSHVLECVMCAALSSVKKEKLQEANDVLTLFPRLRPLVASMGWDLLPGKTATRRKLMRLLWTSDSQALRLEESSLYGNQTDELELASFAACVNSGKSWTPKASFLMHGNVSSAHDDAEVDPFVENLVLERLSAQSPLRVLFDVVPGIKFQDAISLISMQPIASTAEAWKRIEDIELMHMRYALEAIVLALGAMEEAMKDETDASHRVVFYHLKDLTNHLEAIKNVPRKIMMVNIVISLLHIDDIRLSSTQSASSACFSEKSNTPGLDPGDLGTEGEKEIVISFTKQLLDVLRRNLPSHPIEQECQLDGNYSTDGRQALEWRVSMAKRFIEDCEWRLSVMQHLLPLSERQWGLKEVLSILRAAPEKLLNLCMQRAKYDIGEEAVNRFALSAEDKATLELAEWVDNAFKGTLVEDVMSRTAEGAAAVQDLDFHSLGSQLSPLAMILLCIDAAASSAKSPTISKQLLDKSQVMLSEIYPGGAPKVGFTYWDQVHEVAIISVLRRILKRLQEFLEQDDPQILQASFSGDTIISSCTESHRQGQKDRALAMLHQMIEDAHRGKRQFLSGKLHNLARALADEKPEVDVLKGDGSDMAVEKDGVLGLGLKYTKQSPGSANRAVDGNPVSHETEDKGKKSFGPLSNKTSTYLSQFILYTAAIGDIVDGTDTTHDFNFFSLVYEWPKDLLTRLVFDRSSTDAAAKVAEVMSADFVHEVISACVPPVYPPRSGHGWACIPVIPTTPCSHSEGKVLSPSIEAKPNCYVRSSATPGVPLYPLQLDVIRHLVKISPVRAVLACVFGGSILYNGSDSIISSSLNDEFPSSPDADRLFYEFSLDQSERYPTLNRWIQMQTNLHRVSEFVVTPKQKPDDTRIKPDERTGIKRLLEHDSDSESDTEETFSKNNIQPALTDGSARDGGSFENGVCRTDPTVFLSFDWENEVPYEKAVNRLIDEGKLMDALALSDRFLRNGASDWLLQLLIKSREENPSTSGRSQGYGGQSNSWQYCLRLKDKQLAATLALKYMHTWELEAALNVLTMCSCHLLESDPISLEVLHRRQALQKYSHILSADDRHNSWQEVEAECKEDPEGLALRLAGKGAVSAALEVAESAGLSIDLRRELQGRQLVKLLTTDPLNGGGPAEASRFLSSLQDSADALPVVMGAMQLLPDLRSKQLLVHFFLKRRDSNLSDLEVARLNSWALGLKVLAALPLPWQQRCSSLHEHPNLIFEALLMRKQLQYASLILKEFPALRDNNVIMAYAAKAISVTIIPPPREPRITVSASRLRQKSRAGPAVKASFTSSLSNFQREARRAFSWAPRNAENRTTSKDVYRKRKNSGLGASERAAWEAMTGIQEDQGSSYSADGQDRLPSVSIAEEWMLTGDKTKDEGVRASHKYESTPDIILFKALLSLCSDELVSARSAMDLCISQMKNVLSSKQLSEGASVETIGRAYHATEAFVQGLSYAKSLLRKLLGTTESTNNNGERSRDVDDISSDAGSSSVGSQSTDEPSDVLSLTEIWLGRAELLQSLLGSGISTSLDDIADQLSSECLRDRLISDERYSMAVYMCKKCKIDVFPVWKAWGLALLRMERYAQARVKFKQAFQLKGEDIPDVIQEIINTIEGGPPVDVSIVRSMYDHLAKSAPTILDDSLSADSYLNVLHMPSTFPRSERSRRSLESEKNSSVPGSDFEDGPRSNLDTTRYSECTNYLQEHARQNLLGFMFRHGHFKDACMLFFPQSGLPPPLQTSSVGAVSTSSSPQRTDPLATEYGTIESLCEFCVGYGAISSLEEVITERLESAKNQDQAINQYIAGALTRICAFFEINRHFNYLYKFLVLKKDYVTSGYCCIQLFMNSTTQEDAVRHLEHAKAHFEEALTARHRGSDSKKLVTKGVRGKSAAEKLSEETLVKLSSRVKMQIDVVKSFSDSEGAPWKHSLFGNPNDSETSRRRCEIVETLVEKNFDLAYSVIYEFKLSAVDIYAGVATSLADRKKGSQLTELFKNIKGTIQDDDWDQVLGAAINIYANKHKERPDRLIDMLTSSHRKVLACVVCGRLKSAFQIASKSGSVADVQYVAHQALHANSHTVLDMCKQWLAKYM

Target ORF information:

RefSeq Version	NM_001336018.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana zinc finger FYVE domain protein (AT2G25730), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336018.1

ORF Insert Sequence:

ATGGATAAAG AGACGGAGAT TCTCTCCCGT CTCGCGGCGA ACCACCTTCA TCTGGCTCAA 
TTCGAGCCAT TGAAGGCTAC GTTACTCGCT CTCAGGGTTC GTAACCCTGA CCTCGCACTC 
ACCATTCTCC AAACCATCGT CTCCAACGCT GGAAGATTCG ATAATGTCCT CTGGTCACGC 
TCTTGTCCTT CCCCGTCTCT TCTCTCGTTC CTCTCCACGA TTGAGCTTCT GAGATTCGAA 
AATCCTACTT CTCCTTGGGG ATTTGATTCA GAAACTCTAA GTTTGCGTGC CGATTTCTTG 
TTGATGGTTC AGGTTTTGAT CGATAGAGTT ACAGAGAGGA TTAAGGAAGA TGAGGAGAGT 
GAGGATGAAA ATTCTGGATT AGGGAATTGT TTAAGGGTGT TGCAAGGTGT TTTGGAGTTA 
GGTGTTGAGA GGTTGAAGTT TGTTGTTGAT ACTAGTAGTA GTGAAGGAAG TAATAAGATT 
GAGGAAGATG CAGTTGTGTC TTTGAGGAGT ATAGTATTGG ATTACTCTGA TGTTTTCGAT 
GCTTTATGTT GTAATATTCA GAGGCAACTT GCGGGTTGCG AGAGTTACGG TACATGTTTG 
GTTGAGGAAG TTCAGGGAGA AGAACAGAGA AAGGAGATGA ATGAGGCCAC ATGTATTGGT 
TCTCCGGAGC TGGATAACAT CAATGTGTTT GCTTTGATAC AGAGGAATGT TCAGTTAGCA 
CAGTTGGATG CTATGAAAAC AAAGTTGGAT GAAGGTGATG AGCGCGGGGC AGCTGATCGC 
ATTCGTTATC TTCACCTTGA TTATGGAGTA GAGAAAGAGA ACTATCATGC TGTTCTAAAA 
GCTCTCCTTT CAAGAGTTAT GGAGAAAAAG GATGAATATG GTGATTCCTG GCACATGGTG 
CGCCAGAACT TGCTGTTTAT GTATAAAGAA GCTCTCTCAT CGAATTGTGG AGATCTTGTT 
CAGATGATCC AGGGTATTCA AGATGATATG CTCCTCCCAC ATAGCCAACT ACATTTATCT 
CTCGACAATG AACAAATTCC ACTCCCTCTT GAATGTTTCC GGCGATATCT TGTAGACTTG 
AAAACTGAGA GAAATATAGA GGACAAAAGT TCTCCTATGA GCAGGGCAAT TAATTCTTGT 
CTCAGAGATA TGTATCATTA TGCTCGTATT TCTGGATCAC ATGTTCTTGA GTGTGTGATG 
TGTGCTGCTT TGTCTTCTGT AAAGAAAGAG AAGCTTCAGG AGGCTAATGA TGTTCTTACT 
TTGTTTCCCC GACTTCGCCC TTTAGTAGCC TCCATGGGTT GGGATCTATT GCCGGGCAAA 
ACTGCAACCC GTAGAAAATT GATGCGGCTA CTTTGGACTA GTGACTCGCA AGCACTTCGG 
CTAGAAGAAT CTTCTCTTTA TGGAAACCAG ACAGATGAAC TGGAACTTGC ATCTTTCGCT 
GCTTGTGTCA ATTCTGGTAA ATCATGGACT CCAAAGGCAT CTTTCTTGAT GCATGGTAAT 
GTGTCATCCG CGCATGATGA TGCGGAGGTG GATCCTTTTG TTGAAAATCT TGTATTGGAA 
AGGCTTTCAG CGCAAAGTCC ACTTCGGGTA TTGTTTGACG TTGTTCCGGG CATAAAATTC 
CAAGATGCTA TTTCACTGAT TAGTATGCAA CCTATTGCTT CAACTGCAGA AGCCTGGAAG 
AGGATAGAAG ATATTGAACT GATGCATATG CGTTATGCTC TGGAGGCAAT CGTTTTAGCA 
CTAGGTGCAA TGGAAGAGGC TATGAAGGAT GAGACAGATG CTAGTCATCG AGTAGTATTT 
TACCATTTAA AAGACCTCAC TAACCATTTG GAGGCCATTA AAAATGTTCC ACGCAAGATA 
ATGATGGTGA ACATAGTTAT TTCACTCTTA CATATTGATG ATATCCGTCT CAGTTCTACG 
CAAAGTGCCT CCTCGGCATG TTTTTCTGAA AAAAGTAACA CACCTGGTTT GGATCCTGGC 
GATCTTGGTA CAGAAGGGGA AAAGGAAATT GTTATTTCTT TCACAAAACA GCTACTCGAT 
GTTTTACGCC GCAATCTTCC ATCACATCCA ATTGAACAAG AGTGTCAGCT GGATGGTAAT 
TACAGTACTG ATGGAAGACA GGCTTTAGAA TGGAGAGTAT CCATGGCTAA GCGTTTCATT 
GAAGATTGTG AATGGCGATT ATCTGTTATG CAGCATCTTC TGCCACTTTC TGAACGCCAG 
TGGGGTTTAA AGGAGGTTTT GAGTATTCTA AGGGCAGCCC CTGAAAAACT GCTTAATCTC 
TGTATGCAAA GAGCTAAGTA TGACATTGGA GAAGAGGCAG TTAATCGGTT TGCGTTATCA 
GCAGAGGACA AAGCTACTCT TGAATTAGCT GAATGGGTTG ATAATGCGTT CAAAGGAACA 
CTGGTAGAAG ATGTAATGTC TCGTACTGCT GAAGGAGCAG CTGCCGTGCA AGATTTAGAT 
TTTCATTCTT TAGGTTCTCA ATTGAGTCCA TTGGCTATGA TTCTACTGTG CATAGATGCG 
GCTGCTTCTT CTGCCAAGTC TCCAACAATT TCCAAACAAC TTTTGGATAA GTCTCAAGTT 
ATGTTATCGG AAATTTACCC TGGAGGAGCT CCGAAGGTGG GGTTTACTTA CTGGGATCAG 
GTCCACGAAG TTGCAATAAT TTCTGTATTG CGAAGGATCT TAAAGCGTCT GCAGGAATTC 
CTTGAACAGG ATGACCCTCA AATTCTTCAA GCCAGTTTTA GTGGAGATAC CATAATTTCA 
TCTTGCACGG AATCTCATAG ACAGGGACAA AAAGATCGTG CTCTTGCAAT GCTACATCAA 
ATGATTGAGG ATGCTCATAG GGGCAAGCGT CAGTTCCTGA GTGGTAAGCT TCATAACTTA 
GCGAGAGCAC TCGCTGATGA AAAACCAGAA GTTGACGTAC TCAAAGGGGA CGGATCAGAC 
ATGGCCGTTG AGAAGGATGG AGTTCTTGGT CTTGGGCTAA AATATACAAA GCAAAGTCCT 
GGTTCAGCAA ATAGAGCCGT GGATGGAAAT CCTGTTTCAC ATGAAACAGA AGACAAGGGA 
AAGAAGTCAT TTGGCCCATT AAGCAACAAA ACCTCTACTT ATCTATCTCA GTTTATCCTC 
TATACTGCTG CTATTGGTGA TATAGTAGAT GGAACTGACA CAACCCATGA TTTCAACTTT 
TTCTCTCTTG TTTATGAATG GCCTAAAGAC CTATTGACGC GTCTGGTTTT TGATCGAAGT 
AGCACAGATG CAGCTGCAAA AGTTGCTGAG GTTATGTCTG CTGATTTTGT TCATGAAGTG 
ATATCAGCAT GTGTTCCCCC AGTTTATCCC CCACGTTCTG GTCATGGGTG GGCTTGTATT 
CCCGTCATTC CAACCACTCC ATGTTCCCAC TCAGAGGGTA AAGTGCTCTC TCCTTCAATA 
GAGGCTAAAC CCAACTGTTA TGTCCGTTCC TCAGCAACAC CTGGTGTCCC TCTGTATCCT 
CTTCAGTTGG ATGTTATCAG GCATTTGGTA AAAATTTCAC CAGTACGAGC AGTTTTAGCT 
TGCGTCTTTG GTGGGAGCAT ATTGTACAAT GGCAGTGATT CTATCATATC TAGCTCCTTG 
AACGATGAGT TTCCAAGTTC TCCTGATGCA GACAGATTGT TTTATGAATT TTCTCTTGAT 
CAGTCTGAGA GGTATCCCAC TTTAAACCGA TGGATACAGA TGCAGACTAA TCTGCATCGA 
GTTTCGGAAT TTGTTGTGAC ACCGAAGCAA AAACCTGATG ACACACGGAT TAAGCCTGAT 
GAAAGAACTG GGATCAAGAG ACTTCTTGAA CATGATAGTG ACTCAGAGTC AGATACAGAA 
GAAACATTTT CTAAAAATAA CATTCAACCA GCATTGACAG ACGGCAGTGC TCGTGATGGT 
GGATCCTTTG AAAATGGAGT TTGTAGAACT GATCCTACCG TTTTCCTTTC TTTTGATTGG 
GAGAATGAAG TACCGTATGA GAAAGCTGTA AATAGACTAA TTGATGAAGG AAAACTAATG 
GATGCTTTAG CACTTTCGGA CCGCTTCTTG CGGAATGGGG CTTCTGATTG GTTACTTCAG 
CTCCTAATCA AAAGTAGAGA AGAAAATCCT TCAACATCAG GACGATCTCA GGGATATGGA 
GGCCAGAGCA ACAGTTGGCA GTATTGCCTG CGGCTAAAGG ACAAACAGCT GGCAGCAACA 
CTGGCTCTTA AGTATATGCA TACGTGGGAG CTTGAGGCAG CTTTAAATGT TCTTACAATG 
TGTAGTTGTC ATCTCTTAGA AAGCGATCCA ATTAGTCTTG AGGTGTTGCA TAGGAGACAA 
GCTCTGCAGA AGTACAGCCA CATACTTTCG GCAGATGATC GCCATAATAG CTGGCAAGAG 
GTAGAAGCCG AATGTAAAGA AGACCCTGAA GGCTTGGCTC TAAGATTGGC TGGGAAAGGA 
GCTGTTTCCG CTGCGCTAGA AGTGGCTGAA AGTGCAGGAT TATCAATAGA TCTTAGAAGA 
GAATTACAAG GACGGCAGCT TGTAAAGCTT CTAACCACTG ATCCACTCAA TGGTGGTGGT 
CCGGCGGAAG CATCTCGGTT TTTATCTTCA CTTCAAGACT CGGCTGATGC TCTACCAGTG 
GTTATGGGTG CAATGCAATT ATTACCTGAC CTTCGGTCGA AACAGCTCCT GGTCCATTTC 
TTTCTCAAGC GAAGAGATAG CAATCTGTCG GATTTGGAAG TCGCCCGGCT TAATTCTTGG 
GCTTTAGGTC TGAAAGTGTT AGCTGCATTA CCACTTCCTT GGCAACAGAG ATGCTCTTCG 
CTTCATGAGC ATCCCAATTT AATATTTGAA GCTCTGCTAA TGAGAAAACA ATTACAATAC 
GCCTCACTGA TACTCAAAGA GTTCCCAGCA TTAAGAGATA ACAATGTTAT CATGGCCTAT 
GCAGCAAAAG CTATTTCTGT GACAATTATC CCACCACCAA GAGAACCTCG AATAACTGTG 
TCTGCGTCAA GGTTAAGACA GAAATCAAGA GCAGGGCCAG CAGTAAAAGC ATCCTTCACT 
AGTAGCTTAA GCAATTTTCA GAGAGAGGCT CGAAGGGCAT TCTCATGGGC TCCACGTAAT 
GCTGAAAACC GGACGACGTC AAAGGATGTT TATCGCAAAA GAAAGAATTC CGGACTGGGG 
GCTTCTGAGA GAGCTGCATG GGAGGCGATG ACAGGTATTC AAGAGGACCA GGGATCATCT 
TATTCGGCAG ATGGGCAAGA TAGGCTACCT TCTGTTTCTA TTGCTGAAGA ATGGATGCTA 
ACTGGCGACA AAACCAAAGA TGAAGGTGTT CGTGCATCTC ACAAGTATGA AAGCACCCCC 
GATATTATTC TCTTTAAGGC TCTACTATCA CTTTGTTCAG ATGAGCTAGT ATCAGCAAGA 
AGTGCCATGG ACCTATGCAT CAGTCAAATG AAAAACGTAT TGAGCTCCAA ACAGTTGTCA 
GAGGGTGCGT CTGTTGAAAC AATTGGCCGA GCATATCATG CAACGGAGGC ATTTGTGCAG 
GGTTTGTCGT ATGCAAAATC ATTGCTGAGA AAGCTTCTAG GTACCACTGA ATCGACCAAT 
AACAATGGTG AAAGAAGTAG AGATGTTGAT GATATATCTT CTGATGCTGG CAGCTCCAGT 
GTTGGCAGTC AGTCAACGGA TGAACCATCG GATGTTCTCT CACTTACAGA AATCTGGTTG 
GGGCGCGCAG AGTTGCTGCA GAGCCTCTTA GGGTCTGGAA TTTCTACTTC TCTTGATGAC 
ATTGCTGATC AATTGTCATC CGAATGTCTA CGAGACAGAT TAATATCTGA TGAACGATAT 
AGTATGGCGG TGTATATGTG CAAGAAATGC AAGATTGATG TTTTCCCCGT GTGGAAAGCA 
TGGGGTCTTG CTTTGTTACG GATGGAGCGC TATGCTCAAG CTCGAGTCAA ATTCAAGCAA 
GCCTTTCAAT TAAAGGGGGA AGACATTCCT GATGTCATTC AGGAGATAAT AAATACAATA 
GAAGGAGGTC CGCCTGTGGA TGTGTCAATT GTACGTTCCA TGTATGACCA TTTGGCGAAA 
AGCGCACCTA CAATTTTGGA CGATTCTTTA TCAGCAGACT CATACCTAAA TGTTCTGCAT 
ATGCCATCCA CTTTCCCTCG TTCAGAGAGG TCGCGGAGAT CTCTGGAATC GGAAAAGAAT 
AGTTCTGTAC CTGGTTCAGA CTTTGAAGAT GGGCCCCGAA GCAACTTGGA TACTACACGC 
TATTCTGAGT GCACCAACTA CTTGCAGGAA CATGCTCGCC AAAACCTGCT TGGGTTTATG 
TTCCGCCATG GTCACTTTAA AGATGCATGC ATGTTATTCT TTCCGCAAAG TGGTCTTCCC 
CCTCCTTTGC AAACTTCATC TGTGGGCGCA GTAAGCACAT CTTCATCACC TCAACGAACT 
GATCCCTTGG CAACTGAATA TGGGACCATT GAAAGTTTGT GCGAGTTCTG TGTTGGTTAT 
GGAGCTATCT CATCCCTTGA GGAAGTAATT ACAGAAAGAC TTGAATCCGC AAAGAATCAA 
GATCAAGCCA TAAATCAGTA CATAGCTGGA GCTCTTACTC GTATCTGTGC TTTCTTTGAG 
ATCAACCGGC ATTTCAATTA CCTATACAAG TTTCTGGTAC TCAAGAAGGA TTATGTCACC 
TCTGGGTATT GTTGTATTCA GCTTTTTATG AATTCTACAA CTCAGGAGGA TGCTGTAAGG 
CATCTTGAGC ATGCAAAGGC ACACTTTGAA GAAGCATTGA CAGCGCGTCA TAGAGGTTCA 
GACTCAAAAA AACTTGTTAC AAAGGGTGTT AGAGGAAAAA GTGCCGCAGA GAAGCTGAGT 
GAAGAAACTC TTGTTAAGTT GTCCTCACGG GTGAAAATGC AGATTGATGT GGTGAAGTCC 
TTCAGTGACT CTGAAGGAGC ACCATGGAAG CATTCCTTGT TTGGAAATCC AAATGATTCA 
GAGACATCCA GGAGAAGATG TGAAATAGTG GAGACTCTTG TTGAGAAAAA TTTCGACTTA 
GCTTATTCTG TTATATATGA ATTCAAGCTC TCAGCTGTTG ATATATATGC TGGTGTTGCT 
ACGTCACTAG CTGATAGGAA GAAAGGCAGT CAGTTGACAG AACTTTTCAA AAACATTAAG 
GGAACAATCC AGGATGATGA CTGGGATCAG GTCCTGGGTG CTGCCATCAA TATATATGCC 
AACAAGCACA AAGAGCGCCC TGACCGTCTC ATCGACATGT TAACAAGCAG CCATCGAAAG 
GTGCTGGCTT GTGTGGTATG TGGCCGCCTG AAAAGCGCAT TCCAGATTGC ATCTAAAAGC 
GGAAGCGTGG CTGATGTTCA ATATGTAGCT CATCAAGCCT TACATGCCAA TTCGCACACA 
GTACTCGATA TGTGCAAGCA ATGGCTAGCT AAATACATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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AT2G25730 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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