CLF cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLF
Entrez Gene ID	816870
Full Name	SET domain-containing protein
Synonyms	CURLY LEAF,F26B6.3,F26B6_3,ICU1,INCURVATA 1,SDG1,SET1,SETDOMAIN 1,SETDOMAIN GROUP 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001335847.1	NP_001324816.1	SET domain-containing protein
NM_127902.6	NP_179919.1	SET domain-containing protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0275500	NP_001057379.1
Arabidopsis thaliana (thale cress)	CLF	NP_179919.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that CURLY LEAF (CLF), a major catalytic subunit of PRC2, coordinates with different members of PRC1 in suppression of distinct plant developmental programs.
Title: Arabidopsis Flower and Embryo Developmental Genes are Repressed in Seedlings by Different Combinations of Polycomb Group Proteins in Association with Distinct Sets of Cis-regulatory Elements.

CLF may also regulate homologous recombination via other mechanisms.
Title: Polycomb-group histone methyltransferase CLF is required for proper somatic recombination in Arabidopsis.

Antagonistic roles of SEPALLATA3, FT and FLC genes as targets of the polycomb group gene CURLY LEAF
Title: Antagonistic roles of SEPALLATA3, FT and FLC genes as targets of the polycomb group gene CURLY LEAF.

CLF is required for the temporally regulated termination of floral stem cell maintenance.
Title: AGAMOUS terminates floral stem cell maintenance in Arabidopsis by directly repressing WUSCHEL through recruitment of Polycomb Group proteins.

Loss of CLF function increases meristematic activity in Arabidopsis.
Title: The CHD3 chromatin remodeler PICKLE and polycomb group proteins antagonistically regulate meristem activity in the Arabidopsis root.

The following CLF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb07779	NM_127902.6 Latest version!	Arabidopsis thaliana SET domain-containing protein (CLF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb35925	NM_001335847.1 Latest version!	Arabidopsis thaliana SET domain-containing protein (CLF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLF ( NM_127902.6 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_179919.1 Arabidopsis thaliana SET domain-containing protein (CLF), mRNA.

Price & Availability↑

CloneID	OAb07779
Clone ID Related Accession (Same CDS sequence)	NM_127902.6
Accession Version	NM_127902.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	SET domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_127902.5.

ATGGCGTCAG AAGCTTCGCC TTCTTCTTCG GCCACCAGAT CGGAGCCACC CAAAGACTCT 
CCGGCGGAGG AGAGAGGTCC AGCTTCTAAG GAAGTATCAG AAGTAATAGA ATCGCTAAAG 
AAGAAGCTTG CAGCTGATAG GTGTATATCA ATAAAGAAAA GGATTGATGA AAACAAGAAG 
AATTTGTTTG CTATTACTCA AAGTTTTATG AGGTCTTCTA TGGAACGAGG AGGTAGCTGT 
AAAGATGGCA GTGATCTTTT AGTTAAGAGG CAAAGAGATT CGCCAGGTAT GAAAAGCGGA 
ATCGATGAAA GTAATAACAA CAGATATGTA GAAGATGGAC CTGCCAGTTC AGGAATGGTT 
CAAGGATCTA GTGTCCCTGT CAAAATTTCC TTACGTCCTA TCAAAATGCC TGATATCAAA 
CGTTTGTCAC CTTATACCAC ATGGGTTTTT CTGGACAGAA ATCAAAGAAT GACTGAAGAC 
CAGTCTGTAG TGGGTCGAAG GAGAATTTAT TATGATCAAA CTGGCGGGGA AGCGCTTATC 
TGCAGTGATA GTGAAGAGGA GGCCATTGAC GACGAAGAAG AAAAAAGAGA TTTTTTGGAG 
CCTGAAGATT ATATTATTCG CATGACCCTT GAGCAACTAG GTCTTTCAGA CTCAGTCCTG 
GCGGAACTAG CAAGTTTCTT GTCTAGAAGT ACTAGTGAAA TCAAGGCAAG ACATGGAGTG 
CTTATGAAGG AAAAAGAAGT ATCCGAGAGT GGCGATAATC AAGCAGAGAG CTCCCTTCTC 
AACAAAGATA TGGAAGGAGC ATTAGATTCT TTCGATAACC TGTTCTGCCG TAGATGCCTT 
GTATTTGATT GCCGGCTTCA TGGGTGTTCA CAGGATCTCA TTTTTCCGGC TGAGAAACCA 
GCTCCATGGT GTCCTCCTGT AGATGAAAAT TTAACCTGTG GTGCAAACTG CTATAAAACG 
CTTCTCAAGT CTGGAAGATT TCCGGGATAT GGCACCATTG AAGGTAAAAC TGGCACTTCA 
TCAGATGGTG CAGGTACTAA AACCACACCC ACGAAGTTCT CCAGCAAACT TAATGGGAGA 
AAACCAAAGA CCTTCCCAAG TGAAAGTGCA TCGTCTAATG AAAAGTGCGC ACTAGAAACA 
AGTGACTCAG AGAATGGACT ACAGCAGGAT ACCAATTCCG ATAAAGTTTC ATCATCGCCA 
AAGGTGAAAG GTAGTGGGAG ACGAGTAGGT CGTAAGAGGA ACAAAAACCG AGTTGCTGAG 
CGAGTTCCTC GTAAGACTCA GAAGAGGCAG AAGAAGACAG AAGCCTCGGA TAGTGATTCC 
ATCGCCAGTG GAAGTTGTTC ACCCAGCGAT GCAAAACATA AAGATAATGA AGATGCTACT 
TCCTCTTCTC AGAAGCATGT AAAATCTGGG AACTCCGGGA AGTCAAGGAA GAATGGCACT 
CCTGCCGAAG TCTCCAATAA TTCTGTGAAG GATGACGTTC CTGTTTGCCA GTCAAATGAG 
GTTGCGTCAG AGCTTGATGC GCCGGGTAGT GATGAAAGTC TAAGGAAAGA AGAGTTTATG 
GGTGAAACTG TATCTCGAGG AAGATTGGCT ACAAATAAGT TGTGGAGACC ACTTGAGAAA 
AGCCTTTTTG ATAAAGGTGT TGAGATTTTT GGAATGAATA GCTGCTTGAT TGCTAGAAAT 
CTTTTGAGTG GTTTCAAATC ATGTTGGGAG GTCTTCCAAT ACATGACGTG CTCGGAAAAT 
AAAGCTTCCT TCTTTGGAGG TGATGGATTG AATCCTGATG GCTCTTCCAA GTTCGATATC 
AATGGAAATA TGGTTAATAA CCAAGTGAGG AGAAGGTCAA GATTTCTACG TAGGAGAGGC 
AAAGTGCGGC GCTTGAAGTA TACCTGGAAG TCTGCTGCAT ATCATTCAAT TAGGAAAAGA 
ATTACTGAGA AGAAAGACCA GCCCTGCCGT CAGTTTAATC CATGTAACTG CAAAATTGCT 
TGTGGGAAGG AATGTCCTTG TTTGCTAAAC GGGACTTGCT GCGAGAAGTA CTGCGGTTGC 
CCAAAGAGCT GCAAGAATAG GTTTAGAGGT TGTCATTGTG CCAAAAGTCA GTGTCGAAGC 
CGCCAGTGTC CATGCTTTGC TGCAGATCGG GAATGTGACC CAGATGTTTG TAGAAACTGC 
TGGGTCATTG GTGGGGATGG TTCGCTTGGG GTCCCAAGCC AAAGAGGCGA TAATTATGAG 
TGCAGGAATA TGAAATTGCT CCTGAAACAA CAACAAAGGG TTTTACTTGG AATATCTGAT 
GTTTCTGGTT GGGGAGCTTT CTTAAAGAAC AGTGTAAGTA AGCATGAATA CCTTGGGGAA 
TACACAGGAG AGCTGATCTC ACATAAAGAG GCAGATAAAC GCGGGAAGAT ATACGATCGC 
GAGAACTGCT CTTTTCTCTT CAATCTAAAC GATCAGTTTG TGCTAGATGC TTACAGGAAA 
GGAGATAAAC TGAAATTCGC CAACCATTCT CCTGAACCTA ACTGTTACGC AAAGGTCATC 
ATGGTTGCTG GAGATCACAG GGTGGGGATC TTCGCAAAAG AGAGGATACT GGCTGGAGAA 
GAACTATTTT ACGATTACCG GTATGAGCCA GATCGAGCTC CAGCTTGGGC CAAAAAACCT 
GAAGCTCCTG GTTCTAAGAA AGACGAAAAT GTTACACCTT CTGTTGGTAG ACCCAAGAAG 
CTTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_179919.1
CDS	479..3187
Translation	MASEASPSSSATRSEPPKDSPAEERGPASKEVSEVIESLKKKLAADRCISIKKRIDENKKNLFAITQSFMRSSMERGGSCKDGSDLLVKRQRDSPGMKSGIDESNNNRYVEDGPASSGMVQGSSVPVKISLRPIKMPDIKRLSPYTTWVFLDRNQRMTEDQSVVGRRRIYYDQTGGEALICSDSEEEAIDDEEEKRDFLEPEDYIIRMTLEQLGLSDSVLAELASFLSRSTSEIKARHGVLMKEKEVSESGDNQAESSLLNKDMEGALDSFDNLFCRRCLVFDCRLHGCSQDLIFPAEKPAPWCPPVDENLTCGANCYKTLLKSGRFPGYGTIEGKTGTSSDGAGTKTTPTKFSSKLNGRKPKTFPSESASSNEKCALETSDSENGLQQDTNSDKVSSSPKVKGSGRRVGRKRNKNRVAERVPRKTQKRQKKTEASDSDSIASGSCSPSDAKHKDNEDATSSSQKHVKSGNSGKSRKNGTPAEVSNNSVKDDVPVCQSNEVASELDAPGSDESLRKEEFMGETVSRGRLATNKLWRPLEKSLFDKGVEIFGMNSCLIARNLLSGFKSCWEVFQYMTCSENKASFFGGDGLNPDGSSKFDINGNMVNNQVRRRSRFLRRRGKVRRLKYTWKSAAYHSIRKRITEKKDQPCRQFNPCNCKIACGKECPCLLNGTCCEKYCGCPKSCKNRFRGCHCAKSQCRSRQCPCFAADRECDPDVCRNCWVIGGDGSLGVPSQRGDNYECRNMKLLLKQQQRVLLGISDVSGWGAFLKNSVSKHEYLGEYTGELISHKEADKRGKIYDRENCSFLFNLNDQFVLDAYRKGDKLKFANHSPEPNCYAKVIMVAGDHRVGIFAKERILAGEELFYDYRYEPDRAPAWAKKPEAPGSKKDENVTPSVGRPKKLA

Target ORF information:

RefSeq Version	NM_127902.6
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana SET domain-containing protein (CLF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_127902.6

ORF Insert Sequence:

ATGGCGTCAG AAGCTTCGCC TTCTTCTTCG GCCACCAGAT CGGAGCCACC CAAAGACTCT 
CCGGCGGAGG AGAGAGGTCC AGCTTCTAAG GAAGTATCAG AAGTAATAGA ATCGCTAAAG 
AAGAAGCTTG CAGCTGATAG GTGTATATCA ATAAAGAAAA GGATTGATGA AAACAAGAAG 
AATTTGTTTG CTATTACTCA AAGTTTTATG AGGTCTTCTA TGGAACGAGG AGGTAGCTGT 
AAAGATGGCA GTGATCTTTT AGTTAAGAGG CAAAGAGATT CGCCAGGTAT GAAAAGCGGA 
ATCGATGAAA GTAATAACAA CAGATATGTA GAAGATGGAC CTGCCAGTTC AGGAATGGTT 
CAAGGATCTA GTGTCCCTGT CAAAATTTCC TTACGTCCTA TCAAAATGCC TGATATCAAA 
CGTTTGTCAC CTTATACCAC ATGGGTTTTT CTGGACAGAA ATCAAAGAAT GACTGAAGAC 
CAGTCTGTAG TGGGTCGAAG GAGAATTTAT TATGATCAAA CTGGCGGGGA AGCGCTTATC 
TGCAGTGATA GTGAAGAGGA GGCCATTGAC GACGAAGAAG AAAAAAGAGA TTTTTTGGAG 
CCTGAAGATT ATATTATTCG CATGACCCTT GAGCAACTAG GTCTTTCAGA CTCAGTCCTG 
GCGGAACTAG CAAGTTTCTT GTCTAGAAGT ACTAGTGAAA TCAAGGCAAG ACATGGAGTG 
CTTATGAAGG AAAAAGAAGT ATCCGAGAGT GGCGATAATC AAGCAGAGAG CTCCCTTCTC 
AACAAAGATA TGGAAGGAGC ATTAGATTCT TTCGATAACC TGTTCTGCCG TAGATGCCTT 
GTATTTGATT GCCGGCTTCA TGGGTGTTCA CAGGATCTCA TTTTTCCGGC TGAGAAACCA 
GCTCCATGGT GTCCTCCTGT AGATGAAAAT TTAACCTGTG GTGCAAACTG CTATAAAACG 
CTTCTCAAGT CTGGAAGATT TCCGGGATAT GGCACCATTG AAGGTAAAAC TGGCACTTCA 
TCAGATGGTG CAGGTACTAA AACCACACCC ACGAAGTTCT CCAGCAAACT TAATGGGAGA 
AAACCAAAGA CCTTCCCAAG TGAAAGTGCA TCGTCTAATG AAAAGTGCGC ACTAGAAACA 
AGTGACTCAG AGAATGGACT ACAGCAGGAT ACCAATTCCG ATAAAGTTTC ATCATCGCCA 
AAGGTGAAAG GTAGTGGGAG ACGAGTAGGT CGTAAGAGGA ACAAAAACCG AGTTGCTGAG 
CGAGTTCCTC GTAAGACTCA GAAGAGGCAG AAGAAGACAG AAGCCTCGGA TAGTGATTCC 
ATCGCCAGTG GAAGTTGTTC ACCCAGCGAT GCAAAACATA AAGATAATGA AGATGCTACT 
TCCTCTTCTC AGAAGCATGT AAAATCTGGG AACTCCGGGA AGTCAAGGAA GAATGGCACT 
CCTGCCGAAG TCTCCAATAA TTCTGTGAAG GATGACGTTC CTGTTTGCCA GTCAAATGAG 
GTTGCGTCAG AGCTTGATGC GCCGGGTAGT GATGAAAGTC TAAGGAAAGA AGAGTTTATG 
GGTGAAACTG TATCTCGAGG AAGATTGGCT ACAAATAAGT TGTGGAGACC ACTTGAGAAA 
AGCCTTTTTG ATAAAGGTGT TGAGATTTTT GGAATGAATA GCTGCTTGAT TGCTAGAAAT 
CTTTTGAGTG GTTTCAAATC ATGTTGGGAG GTCTTCCAAT ACATGACGTG CTCGGAAAAT 
AAAGCTTCCT TCTTTGGAGG TGATGGATTG AATCCTGATG GCTCTTCCAA GTTCGATATC 
AATGGAAATA TGGTTAATAA CCAAGTGAGG AGAAGGTCAA GATTTCTACG TAGGAGAGGC 
AAAGTGCGGC GCTTGAAGTA TACCTGGAAG TCTGCTGCAT ATCATTCAAT TAGGAAAAGA 
ATTACTGAGA AGAAAGACCA GCCCTGCCGT CAGTTTAATC CATGTAACTG CAAAATTGCT 
TGTGGGAAGG AATGTCCTTG TTTGCTAAAC GGGACTTGCT GCGAGAAGTA CTGCGGTTGC 
CCAAAGAGCT GCAAGAATAG GTTTAGAGGT TGTCATTGTG CCAAAAGTCA GTGTCGAAGC 
CGCCAGTGTC CATGCTTTGC TGCAGATCGG GAATGTGACC CAGATGTTTG TAGAAACTGC 
TGGGTCATTG GTGGGGATGG TTCGCTTGGG GTCCCAAGCC AAAGAGGCGA TAATTATGAG 
TGCAGGAATA TGAAATTGCT CCTGAAACAA CAACAAAGGG TTTTACTTGG AATATCTGAT 
GTTTCTGGTT GGGGAGCTTT CTTAAAGAAC AGTGTAAGTA AGCATGAATA CCTTGGGGAA 
TACACAGGAG AGCTGATCTC ACATAAAGAG GCAGATAAAC GCGGGAAGAT ATACGATCGC 
GAGAACTGCT CTTTTCTCTT CAATCTAAAC GATCAGTTTG TGCTAGATGC TTACAGGAAA 
GGAGATAAAC TGAAATTCGC CAACCATTCT CCTGAACCTA ACTGTTACGC AAAGGTCATC 
ATGGTTGCTG GAGATCACAG GGTGGGGATC TTCGCAAAAG AGAGGATACT GGCTGGAGAA 
GAACTATTTT ACGATTACCG GTATGAGCCA GATCGAGCTC CAGCTTGGGC CAAAAAACCT 
GAAGCTCCTG GTTCTAAGAA AGACGAAAAT GTTACACCTT CTGTTGGTAG ACCCAAGAAG 
CTTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLF ( NM_001335847.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324816.1 Arabidopsis thaliana SET domain-containing protein (CLF), mRNA.

Price & Availability↑

CloneID	OAb35925
Clone ID Related Accession (Same CDS sequence)	NM_001335847.1
Accession Version	NM_001335847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	SET domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGAACACCG ACCATCTTAT ACAGGCAAGA CATGGAGTGC TTATGAAGGA AAAAGAAGTA 
TCCGAGAGTG GCGATAATCA AGCAGAGAGC TCCCTTCTCA ACAAAGATAT GGAAGGAGCA 
TTAGATTCTT TCGATAACCT GTTCTGCCGT AGATGCCTTG TATTTGATTG CCGGCTTCAT 
GGGTGTTCAC AGGATCTCAT TTTTCCGGCT GAGAAACCAG CTCCATGGTG TCCTCCTGTA 
GATGAAAATT TAACCTGTGG TGCAAACTGC TATAAAACGC TTCTCAAGTC TGGAAGATTT 
CCGGGATATG GCACCATTGA AGGTAAAACT GGCACTTCAT CAGATGGTGC AGGTACTAAA 
ACCACACCCA CGAAGTTCTC CAGCAAACTT AATGGGAGAA AACCAAAGAC CTTCCCAAGT 
GAAAGTGCAT CGTCTAATGA AAAGTGCGCA CTAGAAACAA GTGACTCAGA GAATGGACTA 
CAGCAGGATA CCAATTCCGA TAAAGTTTCA TCATCGCCAA AGGTGAAAGG TAGTGGGAGA 
CGAGTAGGTC GTAAGAGGAA CAAAAACCGA GTTGCTGAGC GAGTTCCTCG TAAGACTCAG 
AAGAGGCAGA AGAAGACAGA AGCCTCGGAT AGTGATTCCA TCGCCAGTGG AAGTTGTTCA 
CCCAGCGATG CAAAACATAA AGATAATGAA GATGCTACTT CCTCTTCTCA GAAGCATGTA 
AAATCTGGGA ACTCCGGGAA GTCAAGGAAG AATGGCACTC CTGCCGAAGT CTCCAATAAT 
TCTGTGAAGG ATGACGTTCC TGTTTGCCAG TCAAATGAGG TTGCGTCAGA GCTTGATGCG 
CCGGGTAGTG ATGAAAGTCT AAGGAAAGAA GAGTTTATGG GTGAAACTGT ATCTCGAGGA 
AGATTGGCTA CAAATAAGTT GTGGAGACCA CTTGAGAAAA GCCTTTTTGA TAAAGGTGTT 
GAGATTTTTG GAATGAATAG CTGCTTGATT GCTAGAAATC TTTTGAGTGG TTTCAAATCA 
TGTTGGGAGG TCTTCCAATA CATGACGTGC TCGGAAAATA AAGCTTCCTT CTTTGGAGGT 
GATGGATTGA ATCCTGATGG CTCTTCCAAG TTCGATATCA ATGGAAATAT GGTTAATAAC 
CAAGTGAGGA GAAGGTCAAG ATTTCTACGT AGGAGAGGCA AAGTGCGGCG CTTGAAGTAT 
ACCTGGAAGT CTGCTGCATA TCATTCAATT AGGAAAAGAA TTACTGAGAA GAAAGACCAG 
CCCTGCCGTC AGTTTAATCC ATGTAACTGC AAAATTGCTT GTGGGAAGGA ATGTCCTTGT 
TTGCTAAACG GGACTTGCTG CGAGAAGTAC TGCGGTTGCC CAAAGAGCTG CAAGAATAGG 
TTTAGAGGTT GTCATTGTGC CAAAAGTCAG TGTCGAAGCC GCCAGTGTCC ATGCTTTGCT 
GCAGATCGGG AATGTGACCC AGATGTTTGT AGAAACTGCT GGGTCATTGG TGGGGATGGT 
TCGCTTGGGG TCCCAAGCCA AAGAGGCGAT AATTATGAGT GCAGGAATAT GAAATTGCTC 
CTGAAACAAC AACAAAGGGT TTTACTTGGA ATATCTGATG TTTCTGGTTG GGGAGCTTTC 
TTAAAGAACA GTGTAAGTAA GCATGAATAC CTTGGGGAAT ACACAGGAGA GCTGATCTCA 
CATAAAGAGG CAGATAAACG CGGGAAGATA TACGATCGCG AGAACTGCTC TTTTCTCTTC 
AATCTAAACG ATCAGTTTGT GCTAGATGCT TACAGGAAAG GAGATAAACT GAAATTCGCC 
AACCATTCTC CTGAACCTAA CTGTTACGCA AAGGTCATCA TGGTTGCTGG AGATCACAGG 
GTGGGGATCT TCGCAAAAGA GAGGATACTG GCTGGAGAAG AACTATTTTA CGATTACCGG 
TATGAGCCAG ATCGAGCTCC AGCTTGGGCC AAAAAACCTG AAGCTCCTGG TTCTAAGAAA 
GACGAAAATG TTACACCTTC TGTTGGTAGA CCCAAGAAGC TTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324816.1
CDS	35..2062
Translation	MNTDHLIQARHGVLMKEKEVSESGDNQAESSLLNKDMEGALDSFDNLFCRRCLVFDCRLHGCSQDLIFPAEKPAPWCPPVDENLTCGANCYKTLLKSGRFPGYGTIEGKTGTSSDGAGTKTTPTKFSSKLNGRKPKTFPSESASSNEKCALETSDSENGLQQDTNSDKVSSSPKVKGSGRRVGRKRNKNRVAERVPRKTQKRQKKTEASDSDSIASGSCSPSDAKHKDNEDATSSSQKHVKSGNSGKSRKNGTPAEVSNNSVKDDVPVCQSNEVASELDAPGSDESLRKEEFMGETVSRGRLATNKLWRPLEKSLFDKGVEIFGMNSCLIARNLLSGFKSCWEVFQYMTCSENKASFFGGDGLNPDGSSKFDINGNMVNNQVRRRSRFLRRRGKVRRLKYTWKSAAYHSIRKRITEKKDQPCRQFNPCNCKIACGKECPCLLNGTCCEKYCGCPKSCKNRFRGCHCAKSQCRSRQCPCFAADRECDPDVCRNCWVIGGDGSLGVPSQRGDNYECRNMKLLLKQQQRVLLGISDVSGWGAFLKNSVSKHEYLGEYTGELISHKEADKRGKIYDRENCSFLFNLNDQFVLDAYRKGDKLKFANHSPEPNCYAKVIMVAGDHRVGIFAKERILAGEELFYDYRYEPDRAPAWAKKPEAPGSKKDENVTPSVGRPKKLA

Target ORF information:

RefSeq Version	NM_001335847.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana SET domain-containing protein (CLF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001335847.1

ORF Insert Sequence:

ATGAACACCG ACCATCTTAT ACAGGCAAGA CATGGAGTGC TTATGAAGGA AAAAGAAGTA 
TCCGAGAGTG GCGATAATCA AGCAGAGAGC TCCCTTCTCA ACAAAGATAT GGAAGGAGCA 
TTAGATTCTT TCGATAACCT GTTCTGCCGT AGATGCCTTG TATTTGATTG CCGGCTTCAT 
GGGTGTTCAC AGGATCTCAT TTTTCCGGCT GAGAAACCAG CTCCATGGTG TCCTCCTGTA 
GATGAAAATT TAACCTGTGG TGCAAACTGC TATAAAACGC TTCTCAAGTC TGGAAGATTT 
CCGGGATATG GCACCATTGA AGGTAAAACT GGCACTTCAT CAGATGGTGC AGGTACTAAA 
ACCACACCCA CGAAGTTCTC CAGCAAACTT AATGGGAGAA AACCAAAGAC CTTCCCAAGT 
GAAAGTGCAT CGTCTAATGA AAAGTGCGCA CTAGAAACAA GTGACTCAGA GAATGGACTA 
CAGCAGGATA CCAATTCCGA TAAAGTTTCA TCATCGCCAA AGGTGAAAGG TAGTGGGAGA 
CGAGTAGGTC GTAAGAGGAA CAAAAACCGA GTTGCTGAGC GAGTTCCTCG TAAGACTCAG 
AAGAGGCAGA AGAAGACAGA AGCCTCGGAT AGTGATTCCA TCGCCAGTGG AAGTTGTTCA 
CCCAGCGATG CAAAACATAA AGATAATGAA GATGCTACTT CCTCTTCTCA GAAGCATGTA 
AAATCTGGGA ACTCCGGGAA GTCAAGGAAG AATGGCACTC CTGCCGAAGT CTCCAATAAT 
TCTGTGAAGG ATGACGTTCC TGTTTGCCAG TCAAATGAGG TTGCGTCAGA GCTTGATGCG 
CCGGGTAGTG ATGAAAGTCT AAGGAAAGAA GAGTTTATGG GTGAAACTGT ATCTCGAGGA 
AGATTGGCTA CAAATAAGTT GTGGAGACCA CTTGAGAAAA GCCTTTTTGA TAAAGGTGTT 
GAGATTTTTG GAATGAATAG CTGCTTGATT GCTAGAAATC TTTTGAGTGG TTTCAAATCA 
TGTTGGGAGG TCTTCCAATA CATGACGTGC TCGGAAAATA AAGCTTCCTT CTTTGGAGGT 
GATGGATTGA ATCCTGATGG CTCTTCCAAG TTCGATATCA ATGGAAATAT GGTTAATAAC 
CAAGTGAGGA GAAGGTCAAG ATTTCTACGT AGGAGAGGCA AAGTGCGGCG CTTGAAGTAT 
ACCTGGAAGT CTGCTGCATA TCATTCAATT AGGAAAAGAA TTACTGAGAA GAAAGACCAG 
CCCTGCCGTC AGTTTAATCC ATGTAACTGC AAAATTGCTT GTGGGAAGGA ATGTCCTTGT 
TTGCTAAACG GGACTTGCTG CGAGAAGTAC TGCGGTTGCC CAAAGAGCTG CAAGAATAGG 
TTTAGAGGTT GTCATTGTGC CAAAAGTCAG TGTCGAAGCC GCCAGTGTCC ATGCTTTGCT 
GCAGATCGGG AATGTGACCC AGATGTTTGT AGAAACTGCT GGGTCATTGG TGGGGATGGT 
TCGCTTGGGG TCCCAAGCCA AAGAGGCGAT AATTATGAGT GCAGGAATAT GAAATTGCTC 
CTGAAACAAC AACAAAGGGT TTTACTTGGA ATATCTGATG TTTCTGGTTG GGGAGCTTTC 
TTAAAGAACA GTGTAAGTAA GCATGAATAC CTTGGGGAAT ACACAGGAGA GCTGATCTCA 
CATAAAGAGG CAGATAAACG CGGGAAGATA TACGATCGCG AGAACTGCTC TTTTCTCTTC 
AATCTAAACG ATCAGTTTGT GCTAGATGCT TACAGGAAAG GAGATAAACT GAAATTCGCC 
AACCATTCTC CTGAACCTAA CTGTTACGCA AAGGTCATCA TGGTTGCTGG AGATCACAGG 
GTGGGGATCT TCGCAAAAGA GAGGATACTG GCTGGAGAAG AACTATTTTA CGATTACCGG 
TATGAGCCAG ATCGAGCTCC AGCTTGGGCC AAAAAACCTG AAGCTCCTGG TTCTAAGAAA 
GACGAAAATG TTACACCTTC TGTTGGTAGA CCCAAGAAGC TTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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CLF cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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