NCU02478 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU02478
Entrez Gene ID	3881212
Full Name	alpha-1,3-glucan synthase Ags2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959979.2	XP_965072.2	alpha-1,3-glucan synthase Ags2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mok11	NP_001018282.1
Schizosaccharomyces pombe (fission yeast)	ags1	NP_588165.3
Schizosaccharomyces pombe (fission yeast)	mok13	NP_596500.1
Neurospora crassa	NCU02478	XP_965072.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU02478 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02478 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf13060	XM_959979.2 Latest version!	Neurospora crassa OR74A alpha-1,3-glucan synthase Ags2 (NCU02478), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU02478 ( XM_959979.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_965072.2 Neurospora crassa OR74A alpha-1,3-glucan synthase Ags2 (NCU02478), mRNA.

Price & Availability↑

CloneID	ONf13060
Clone ID Related Accession (Same CDS sequence)	XM_959979.2
Accession Version	XM_959979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	alpha-1,3-glucan synthase Ags2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959979.1.

ATGGCGGCGG TTCTTCTCAC ACTCGTCCTG GCGACCTTGG TACAAGGGCT CCGGTACGAT 
CCAGACTATG TCGACTACAA CCTTAACCAG AATAAGGACG CCGTGAACCC CTTGGACTAT 
TCTGCCCCGA AACGAGACAA CTACACAGCA TCCCCAAAAA ATTGGAAATT CCCCTTTTAC 
ACGCTTTTCC TCGACAGGTA CGCCAACGGC GACCCAACCA ATGACAACAT CAACGGCACT 
GTCTATGAGC AAGATCCCAC GAGCAACCAG TTGCGGCATG GAGGCGATGT TCAGGGTGTC 
ATTGACAGCC TGGATTACAT CCAGGGCATG GGAATAAAGG CTATATACAT TGCTGGCTCA 
CCGTTTATCA ATCTCCCCTG GGGTGTTGAC TCGTACTCGC CCATCGATTT GACTCTCTTG 
GACATGCATT ACGGCACCAT CAAGGACTGG CAACGCATGG TGGATGAGAT TCACAAACGT 
GATATGTATG TCATGATTGA TCACACCTTC TCAACTATGA GCGATCTTCT GGCATTCAAA 
GGCTTCGAGA ACGAAACCGC ACCCTTTTCA CTCAAAGAAC ACAAGGTGCA CTACAAGGGT 
AATCGAGAGT ACCTCGATTT TGCACCATCC AATGAATACA AGGACGAATG CCAATATCCC 
AGATTTTGGG ATGAAACGGG AATGCCTGTT GGAGAGGACG TCACTAGCCA GCTGAAAGGC 
TGCTACGACA GTGATTTCGA CCAGTATGGT GACATGGAAG CCTTTGGTGT CCATCCGGAC 
TGGCAACGTT CCTTGGCCAA GTTTGCTAGT GTCCAGGATC GGTTGAGGGA CTGGGCACCC 
TCTGTTCGCA AACGCATCGA GTTATTCTCC TGTCTTTCGA TTAGGGTGCT TGATGTTGAC 
GGGTTCCGCT TCGATAAGGC CACGCAAGTC ACGGTCGATG CTATGGCCGA GCATAACGAA 
GCCATCCGCA AGTGTGCCAA AGAACACAAC AAAGACAACT TCTTCATGCC GGGCGAAATC 
ACCGGAGGCG ACGGCTACGG TGCGATATAT ATCGGCAGAG GACGGCAATC GAACCAGAGG 
CCAAGTTTTC CGCAGGTCAT GAATCTGACA ACCAAAGAGC TGAGCAAGAA CAACAGCCTG 
GTTATCCGTG ACGAGGGCAA AAATGGCCTT GACGCTGCTG CTTTTCATTA TTCGGCATAC 
CGGTTTCTGA CCAGATTTGC CGGTATGAGC GGAAACTTGG AAGCTGGTTA CGACCTGCCG 
CTCAATTGGG TTGAGATGTG GAACCAGATG ATCATCACCA ACGATTTCGT CAACCCTAAT 
ACTGGCCTGT TCGATCCACG GCATATGTAC GGAACTATGA ATCAGGACGT CTTCCGGTGG 
TCTGGCATCA AGCAGGGTAC ACAGAGGATG CTTCTGGGTC TATTCATCAT GACTCTGCAT 
ATGCCCGGTA TTCCGTTGGT TTTCTATGGC GAGGAACAAG AATTCTATGT TCTTGACAGC 
ACGGCGGACA ACTACCTTTT CGGAAGACAA GCTTTCTCGC CTACCCCCGG TTGGATGCTC 
CACGGCTGCC ATGTCGGCAA CAGTACCCAA TATGTCGGCT GGCCCATTGA AACATCAAGG 
ACCGGCTGCA CCGACCCCAA GGTTGCTCTG GATCATCGTG ACCCATCGGC TCCCGTCCGC 
AACATTATGA AGTCGATGTA CCACATGCGC GACACGTATG AGACTTTCCG TGAAGCATGG 
CTCCTGCAGA CCCTTAGCTC GAAGGTTAGG AATGTGACGC TCGAAGGTAG CACCACAAGC 
ACAGAGTTTG GGATTTGGTC CGTTGCACGC GCATTTATGC CGAGTGTTCA AAAAGAATCA 
TATCCAGCTG AACCCGTCTG GCTTGTTTAT CACAACGAGG ACAAGGAAAC CGAGTATACA 
TTCGACTGCT CCAAGAAGGG CCAGGACTTC GTCTCTCCAT TCGATGCTGG AGCAACCGTC 
AAGAACTTGT TTTACCCATA TGACGAGATC AAACTGGAGA AATCCACTCA GAGTTTAGGA 
TTTGCTAATT CCCATGCTGT GAGCGGCTGC CTCAGCAATA TCACCATGGC GCCCTTCGAA 
TTCAGAGCGT ATGTACTCAA GGAAGATTGG GAGTCACCCC CGCCTATGAT CACCAAGTTC 
ACTCCTGGCC ACGACGCCTC TGTCGAGTCT TACGGTAACG GCAAGGTCGA TATCCAACTA 
GAGTTTTCGG AGGAAATGGA TTGTGAGGAG GTCTTGAACT CGATCTCCTT CACATCGACA 
ACCGAAGAGA ACGCTAGTAT CAAGATCGAT GCACACCGCT GCGACAATAT CAAAGCCAGC 
TTTATTGCCG AATACGTTGG AACTATCCCT TCGACCTGGA GGTTCAATGC GACCCTGTCC 
AATGTAAAGG ACGGTATTCA CAGGATCACC GTCAAGGACC CCCAGTCCGG CCGTAATGCC 
AGCACGGGCG CCACAGATCA CTTCCTCCTT CGTGTCGGTT CATCCAACAA CCCGCTCGTT 
AACCCCATGA AGGCCAATTA CTCGAAGACG CTGGTGACGA AAGAGGGTAG TGATCTCTTC 
GTCAACCACG CCGCGGCCGG TGCCGATAAG TGGCGCTATT CAACCAACTG GGGCTCGACT 
TGGAGCGACT GGCTCGAGTA TAAGGGAGGC AAGGAGAAGA TCGATAAGCT AGCTTGGAAT 
GGGACCAAGA GACAGGAATG GGACGGTGAC CATGTCATGG TTCAGTACTG GTCCAAGCTA 
CTTGGTAGCG CCTCATTCGT CCAGTCTGGT GACAGCAAAG AGACCAAGAC GCGCCGCTTC 
CCCCACCTCT TCGCCCATGG TCTCTTTAAC AAGTTCGGCT TCGATGCTGG TGTCAAGAAT 
GAGCTGAAGC TGGTTGGTGA CGGCTTGTGG GAGGGCCACG TGATGGATGA GTGGCCCACG 
CACTTCCAGC TCAACGTATG GGGCATGAAT CCCGATCAGA GGCCTGACGC CGGGTGGGTA 
TTTGGCGATG TGGATGGAGA TGGGGTTCTG GATCGTATGC CGCCTTCATC TTTGGCACTA 
AACGTGATCA ATGCTACGGA ACCGCCACCG ATGCCTGCGC TGTCTTGGAA GCTTGTCTTC 
AACGATGCAT TGCTCACTTT CCACAAGGAA CCCCAGGGAA ACGTGGCGGT TCAGATCATT 
ATGTTTATCC TACTGGCTTC ACTCCCCATC GTCGGCGGTC TCACTGCCGT CTGGACTTTC 
ATGGGCTCCT TTTACAAGGT CAAGGTTAAC AAGGTTGGCT TCAAGCGCCG CGGTCGATCA 
CCACTCCGCG ATATTGGCAA GCGCATCAGC AGTACCGTTT CTTTCGAGAA CTTCCGTCAT 
AAGGACGTGG GCGATACAGA GCTCGCTGTC ATCCCAGGCA AGCGCAGAAA GGTCATCATA 
GCCACGATGG AATACAATAT CGACGACTGG AACATTAAGA TCAAGATTGG CGGTCTCGGC 
GTCATGGCCC AGCTCATGGG TAAGGCACTG GAGCACCAGG ATCTGATCTG GGTTGTGCCC 
TGCGTCGGTG GCATCGACTA CCCGATCGAC CAGCGCGCTG AGCCTATGTA CGTGCCCATC 
ATGGGCAAGG AGTACGAAAT CGAAGTGCAG TACCACCAGG TTCAAAATAT CACGTACGTA 
CTGCTCGACG CACCCATCTT CCGCACCCAA TCCAAGGCCG ACCCCTACCC GCCACGCATG 
GACGACATGG ACTCGGCCAT CTACTATGCT GCATGGAACT ACTGTATTGC TGAAGCCATT 
CGTCGGTTCA ACCCGGACCT GTACCACATC AACGACTATC ACGGTGCGGC AGCGCCATTG 
TATTTGCTTC CTGAACGCAC CATTCCATGC GCTCTTTCGC TTCACAATGC CGAATTTCAA 
GGCATGTGGC CTATGCGCAC GCCTGAGGAG TCCAAGGAGG TGTGCGAGGT GTTCAACTTG 
GATCCGGAAA TCGTGAAGGA GTATGTCCAA TTCGGATCCG TCTTCAACCT CCTTCATGCC 
GGAGCAAGCT ATTTGCGCGT TCACCAAAAG GGCTTTGGTG CCGTGGGCGT CTCGAAGAAG 
TATGGCGACC GCTCTTACGC CCGCTATCCC ATCTTCTGGG GTCTGTCCAA GATTGGCCAG 
CTGCCAAACC CTGACCCGAG TGACACGGCC GAGTGGAGCA GAGACGAGCA AATCCAGGCG 
AAGGACGTCG TCGTCGATCT TGAGGCTGAG GCAAAACGCG GTGATTTGAG GAGGCAAGCG 
CAGGAGTGGG CTGGGTTAGA AGTCAACCCG GATGCGGAGC TCTTCGTCTT CGTCGGCAGA 
TGGTCGCTCC AGAAAGGTGT CGATCTCATT GCTGATATCT TCCCGTCCAT CCTGGAAAAG 
TATCCCACCA CCCAGCTCAT TTGCGTCGGC CCGGTTATCG ATCTGTATGG AAAGTTCGCG 
GCACTCAAGT TGGCGAAGCT TATGGAGAAG TATCCCAAGC GCGTTTACAG CAAGCCCGAG 
TTTACCGCCC TGCCTCCTTG TATCTTCAGC GGCGCTGAGT TTGCCCTGAT TCCCTCCCGC 
GACGAACCGT TTGGTCTGGT TGCTGTTGAA TTCGGTCGGA AGGGCGCGCT CGGCGTGGGT 
GCCCGCGTCG GTGGCCTTGG ACAGATGCCT GGTTTCTGGT ACACGATCGA GTCGACCGCT 
CCTCAGCATC TTATTCAGCA ATTCCGTGGA GCCATTGTCA GTGCTTTGGA AAGCAAGAAA 
AAGGACCGTC AGATGATGAG AGCCTGGAGT GCCAAGCAAC GTTTCCCTGT TGCTCAATGG 
GTTGAGGACC TCGACACGCT TCAGACCGAG GCTATTCGCA TTCATCACAA GGAAGCCAAG 
AAGCGGAAGA GAGTTCCTAG TGGCTCTTTG CTCACAGTTC CTTCTACCGT TGATCTTCAC 
GGCAACAATA CCCAGCGTGA TTACTTTGAT GATGCGGTCT CGACCCCTGC GGGCGCACGA 
TCCCGAACGG CATCTACTGT TTCTACTCCG GGGCCTCAAG ACGGTGCAAC ACATCACAGA 
CGAACCCTCT CCAGCCCCTT TGATGATGAC ATTCCCAGCA TTGCGTCACC ACCCCCCGGG 
ACTCCCGGAT CTCCTCCAAC CGTTAACGTG GATCGTGTCG GCGACCATTC AGACCACAAT 
GGTGAAGATG GTGCTGATGA ACCCCTCATG CCACCGAACC CCTTGTTCCT TAACCCCTCT 
GCCAGTTCGT CGGTCACAGA TGTTGCCTCT ATTGCAGCTA ATGTCTACGG CCACGCGGGT 
GACCGCAGTA GTGTCGACAC CTTTGCGATG CGCATGATGT CTCCCGACAG TTCCGAAATT 
CGTCCTCAAG CTTTTGGTCT GCTCAATCCA CAAGCCCGTC CCGGTGCTGA AGCCCTCTAC 
CAAGCACGCA ACCGCAACAG CTCGCGTCTC TCCGTAATTG ATGTCGTTGG TGACAGGCAG 
GATTTCCGCC TGCAAAAGGT TGATCCTTTC TTTACTGATG CCAATGGTCA ATACTACCGC 
GAATTCGAGC GAAAACTCAA CGGGCTCACG GCTAAGAACT CGGAAACCGA ACTCTGCATC 
GAGGATTACC TCAAGGAAAG CGAGAAGGAG TGGTTCAAGG AATTCAGAAA CGCCAAACTG 
GGCCGCAGCC GCAGTCCGAG CCGCTCCCGG AGTCCCATGC CAGGCTTGAA GATCAAGAAG 
ACGAGACACG CGAGCATCGG CAGCGTCCAG AGTATTTCGC CTACCGAAGA CGACGATCGT 
GAGAACGAGG AACGCAACAG CAGCAACAAT GAGGGTGTCG GTCACCATCG CGACGACCAA 
TTTCTGCTTG GCGACGGGTA CAAGCCGCCC ACTGGCTTAA AGAAGCTGCT CTCCATCCGC 
CTTGGCGACT GGCCCATTTA CTCTTTCATC ATTGCACTCG GCCAGATTCT TGCCGCCAAC 
ACATATCAGA TCGTACTACT CGCTGGTGAA TCCGGACAAA CGCCTACACA ACTTTATATC 
GTTGCGGGCA CCTATGCCCT CGGCTCCCTT CTGTGGTGGT TCATGTTCCG CCGCCTGCCG 
GCCCTGTACT CGCTCTCCCT GCCCTGGCTC TTCTATGGCC TGGCATTCAT GCTGCTCGGC 
GTAACGCCGT TTATGCCTGA CCACGTCCGT TTCCCAGTCC AAAACACTGC GTCGGCATTG 
TACGCCGCTG GTGCATCAAG TGGATCGTTG TTCTTTGCAA TGAACTTTGG CGATGAAGGC 
GGTGTTCCCA TAATGACCTG GATTTTCCGC GCTGCCATCA TCCAAGGAAT CCAACAAGTC 
TACATTCTGG CCCTCTGGTA CTGGGGTAGC CTCCTCACCG CGCAAACCCC CACCGGCTCT 
CCGCACGTCG GCACCTGGAT CGCCAACAAG GTCCCCGTCG CCCTCGTTAT CACCGTTCCC 
ATCGCCCTGA TTTTCTGGTG CCTAGGCGTC ATCTCCTTCG TCGGTCTACC TGATTACTAC 
CGCCAACTCC CCGACAAGAT CCCCTCCTTC TACAAGTCAC TCTTGCGCCG CCACATCGTG 
CCCTGGTTTT TGTTGATGGT CGTCATCCAG AACTACTTCC TCTCGGCCCC CTACGGCCGC 
ACCTGGGAGT TCTTGTTTTA TTCCCGCGCC GTTCCCGGCT GGGCCATCTT GCTTCTGGCC 
ATCGGCTTCT TCGTCGGCGT TTGGTGTCTG CTGCTCTGGC TCTTTGCCTA CTTCACCAAA 
ACCCACCCCT GGATCGTCCC CCTCTTTGCC ATCGGCCTCG GCGCCCCGCG CTGGGCACAG 
ATGTTGTGGG CCACCAGTGG AATCGGGCTC TATCTCCCCT GGTGCGGCAC TGTGGTGCTT 
TCGGCGATCA TCTCCCGCTG CTTGTGGCTG TGGTTAGGGC TGCTGGATAC CGTGCAAGGA 
GTGGGGTTGG GTATGGTTTT GCTGCTGACG CTCACGAGGC AGCATGTGGC TGCCACGCTT 
ATTGGGGCGC AGTTTTTGGG GGCCGTGTTC ATGATGCTGG CGAGGGCTAC GGCGCCGGAT 
AAGGATGGAC CGGGAGATGT TTTCCCGGAT TTTAGTGCGG GCGTGATGCC CGGGTTGGGA 
AGGCCGTGGT TTTGGGTTGT CTTGGGGTTG CAGCTTGTGC TGCCGATTGG GTTTTTCAAG 
TTCTTCAGGA AGGAGCAGGT TGCCAAGCCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_965072.2
CDS	352..7524
Translation	MAAVLLTLVLATLVQGLRYDPDYVDYNLNQNKDAVNPLDYSAPKRDNYTASPKNWKFPFYTLFLDRYANGDPTNDNINGTVYEQDPTSNQLRHGGDVQGVIDSLDYIQGMGIKAIYIAGSPFINLPWGVDSYSPIDLTLLDMHYGTIKDWQRMVDEIHKRDMYVMIDHTFSTMSDLLAFKGFENETAPFSLKEHKVHYKGNREYLDFAPSNEYKDECQYPRFWDETGMPVGEDVTSQLKGCYDSDFDQYGDMEAFGVHPDWQRSLAKFASVQDRLRDWAPSVRKRIELFSCLSIRVLDVDGFRFDKATQVTVDAMAEHNEAIRKCAKEHNKDNFFMPGEITGGDGYGAIYIGRGRQSNQRPSFPQVMNLTTKELSKNNSLVIRDEGKNGLDAAAFHYSAYRFLTRFAGMSGNLEAGYDLPLNWVEMWNQMIITNDFVNPNTGLFDPRHMYGTMNQDVFRWSGIKQGTQRMLLGLFIMTLHMPGIPLVFYGEEQEFYVLDSTADNYLFGRQAFSPTPGWMLHGCHVGNSTQYVGWPIETSRTGCTDPKVALDHRDPSAPVRNIMKSMYHMRDTYETFREAWLLQTLSSKVRNVTLEGSTTSTEFGIWSVARAFMPSVQKESYPAEPVWLVYHNEDKETEYTFDCSKKGQDFVSPFDAGATVKNLFYPYDEIKLEKSTQSLGFANSHAVSGCLSNITMAPFEFRAYVLKEDWESPPPMITKFTPGHDASVESYGNGKVDIQLEFSEEMDCEEVLNSISFTSTTEENASIKIDAHRCDNIKASFIAEYVGTIPSTWRFNATLSNVKDGIHRITVKDPQSGRNASTGATDHFLLRVGSSNNPLVNPMKANYSKTLVTKEGSDLFVNHAAAGADKWRYSTNWGSTWSDWLEYKGGKEKIDKLAWNGTKRQEWDGDHVMVQYWSKLLGSASFVQSGDSKETKTRRFPHLFAHGLFNKFGFDAGVKNELKLVGDGLWEGHVMDEWPTHFQLNVWGMNPDQRPDAGWVFGDVDGDGVLDRMPPSSLALNVINATEPPPMPALSWKLVFNDALLTFHKEPQGNVAVQIIMFILLASLPIVGGLTAVWTFMGSFYKVKVNKVGFKRRGRSPLRDIGKRISSTVSFENFRHKDVGDTELAVIPGKRRKVIIATMEYNIDDWNIKIKIGGLGVMAQLMGKALEHQDLIWVVPCVGGIDYPIDQRAEPMYVPIMGKEYEIEVQYHQVQNITYVLLDAPIFRTQSKADPYPPRMDDMDSAIYYAAWNYCIAEAIRRFNPDLYHINDYHGAAAPLYLLPERTIPCALSLHNAEFQGMWPMRTPEESKEVCEVFNLDPEIVKEYVQFGSVFNLLHAGASYLRVHQKGFGAVGVSKKYGDRSYARYPIFWGLSKIGQLPNPDPSDTAEWSRDEQIQAKDVVVDLEAEAKRGDLRRQAQEWAGLEVNPDAELFVFVGRWSLQKGVDLIADIFPSILEKYPTTQLICVGPVIDLYGKFAALKLAKLMEKYPKRVYSKPEFTALPPCIFSGAEFALIPSRDEPFGLVAVEFGRKGALGVGARVGGLGQMPGFWYTIESTAPQHLIQQFRGAIVSALESKKKDRQMMRAWSAKQRFPVAQWVEDLDTLQTEAIRIHHKEAKKRKRVPSGSLLTVPSTVDLHGNNTQRDYFDDAVSTPAGARSRTASTVSTPGPQDGATHHRRTLSSPFDDDIPSIASPPPGTPGSPPTVNVDRVGDHSDHNGEDGADEPLMPPNPLFLNPSASSSVTDVASIAANVYGHAGDRSSVDTFAMRMMSPDSSEIRPQAFGLLNPQARPGAEALYQARNRNSSRLSVIDVVGDRQDFRLQKVDPFFTDANGQYYREFERKLNGLTAKNSETELCIEDYLKESEKEWFKEFRNAKLGRSRSPSRSRSPMPGLKIKKTRHASIGSVQSISPTEDDDRENEERNSSNNEGVGHHRDDQFLLGDGYKPPTGLKKLLSIRLGDWPIYSFIIALGQILAANTYQIVLLAGESGQTPTQLYIVAGTYALGSLLWWFMFRRLPALYSLSLPWLFYGLAFMLLGVTPFMPDHVRFPVQNTASALYAAGASSGSLFFAMNFGDEGGVPIMTWIFRAAIIQGIQQVYILALWYWGSLLTAQTPTGSPHVGTWIANKVPVALVITVPIALIFWCLGVISFVGLPDYYRQLPDKIPSFYKSLLRRHIVPWFLLMVVIQNYFLSAPYGRTWEFLFYSRAVPGWAILLLAIGFFVGVWCLLLWLFAYFTKTHPWIVPLFAIGLGAPRWAQMLWATSGIGLYLPWCGTVVLSAIISRCLWLWLGLLDTVQGVGLGMVLLLTLTRQHVAATLIGAQFLGAVFMMLARATAPDKDGPGDVFPDFSAGVMPGLGRPWFWVVLGLQLVLPIGFFKFFRKEQVAKP

Target ORF information:

RefSeq Version	XM_959979.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A alpha-1,3-glucan synthase Ags2 (NCU02478), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959979.2

ORF Insert Sequence:

ATGGCGGCGG TTCTTCTCAC ACTCGTCCTG GCGACCTTGG TACAAGGGCT CCGGTACGAT 
CCAGACTATG TCGACTACAA CCTTAACCAG AATAAGGACG CCGTGAACCC CTTGGACTAT 
TCTGCCCCGA AACGAGACAA CTACACAGCA TCCCCAAAAA ATTGGAAATT CCCCTTTTAC 
ACGCTTTTCC TCGACAGGTA CGCCAACGGC GACCCAACCA ATGACAACAT CAACGGCACT 
GTCTATGAGC AAGATCCCAC GAGCAACCAG TTGCGGCATG GAGGCGATGT TCAGGGTGTC 
ATTGACAGCC TGGATTACAT CCAGGGCATG GGAATAAAGG CTATATACAT TGCTGGCTCA 
CCGTTTATCA ATCTCCCCTG GGGTGTTGAC TCGTACTCGC CCATCGATTT GACTCTCTTG 
GACATGCATT ACGGCACCAT CAAGGACTGG CAACGCATGG TGGATGAGAT TCACAAACGT 
GATATGTATG TCATGATTGA TCACACCTTC TCAACTATGA GCGATCTTCT GGCATTCAAA 
GGCTTCGAGA ACGAAACCGC ACCCTTTTCA CTCAAAGAAC ACAAGGTGCA CTACAAGGGT 
AATCGAGAGT ACCTCGATTT TGCACCATCC AATGAATACA AGGACGAATG CCAATATCCC 
AGATTTTGGG ATGAAACGGG AATGCCTGTT GGAGAGGACG TCACTAGCCA GCTGAAAGGC 
TGCTACGACA GTGATTTCGA CCAGTATGGT GACATGGAAG CCTTTGGTGT CCATCCGGAC 
TGGCAACGTT CCTTGGCCAA GTTTGCTAGT GTCCAGGATC GGTTGAGGGA CTGGGCACCC 
TCTGTTCGCA AACGCATCGA GTTATTCTCC TGTCTTTCGA TTAGGGTGCT TGATGTTGAC 
GGGTTCCGCT TCGATAAGGC CACGCAAGTC ACGGTCGATG CTATGGCCGA GCATAACGAA 
GCCATCCGCA AGTGTGCCAA AGAACACAAC AAAGACAACT TCTTCATGCC GGGCGAAATC 
ACCGGAGGCG ACGGCTACGG TGCGATATAT ATCGGCAGAG GACGGCAATC GAACCAGAGG 
CCAAGTTTTC CGCAGGTCAT GAATCTGACA ACCAAAGAGC TGAGCAAGAA CAACAGCCTG 
GTTATCCGTG ACGAGGGCAA AAATGGCCTT GACGCTGCTG CTTTTCATTA TTCGGCATAC 
CGGTTTCTGA CCAGATTTGC CGGTATGAGC GGAAACTTGG AAGCTGGTTA CGACCTGCCG 
CTCAATTGGG TTGAGATGTG GAACCAGATG ATCATCACCA ACGATTTCGT CAACCCTAAT 
ACTGGCCTGT TCGATCCACG GCATATGTAC GGAACTATGA ATCAGGACGT CTTCCGGTGG 
TCTGGCATCA AGCAGGGTAC ACAGAGGATG CTTCTGGGTC TATTCATCAT GACTCTGCAT 
ATGCCCGGTA TTCCGTTGGT TTTCTATGGC GAGGAACAAG AATTCTATGT TCTTGACAGC 
ACGGCGGACA ACTACCTTTT CGGAAGACAA GCTTTCTCGC CTACCCCCGG TTGGATGCTC 
CACGGCTGCC ATGTCGGCAA CAGTACCCAA TATGTCGGCT GGCCCATTGA AACATCAAGG 
ACCGGCTGCA CCGACCCCAA GGTTGCTCTG GATCATCGTG ACCCATCGGC TCCCGTCCGC 
AACATTATGA AGTCGATGTA CCACATGCGC GACACGTATG AGACTTTCCG TGAAGCATGG 
CTCCTGCAGA CCCTTAGCTC GAAGGTTAGG AATGTGACGC TCGAAGGTAG CACCACAAGC 
ACAGAGTTTG GGATTTGGTC CGTTGCACGC GCATTTATGC CGAGTGTTCA AAAAGAATCA 
TATCCAGCTG AACCCGTCTG GCTTGTTTAT CACAACGAGG ACAAGGAAAC CGAGTATACA 
TTCGACTGCT CCAAGAAGGG CCAGGACTTC GTCTCTCCAT TCGATGCTGG AGCAACCGTC 
AAGAACTTGT TTTACCCATA TGACGAGATC AAACTGGAGA AATCCACTCA GAGTTTAGGA 
TTTGCTAATT CCCATGCTGT GAGCGGCTGC CTCAGCAATA TCACCATGGC GCCCTTCGAA 
TTCAGAGCGT ATGTACTCAA GGAAGATTGG GAGTCACCCC CGCCTATGAT CACCAAGTTC 
ACTCCTGGCC ACGACGCCTC TGTCGAGTCT TACGGTAACG GCAAGGTCGA TATCCAACTA 
GAGTTTTCGG AGGAAATGGA TTGTGAGGAG GTCTTGAACT CGATCTCCTT CACATCGACA 
ACCGAAGAGA ACGCTAGTAT CAAGATCGAT GCACACCGCT GCGACAATAT CAAAGCCAGC 
TTTATTGCCG AATACGTTGG AACTATCCCT TCGACCTGGA GGTTCAATGC GACCCTGTCC 
AATGTAAAGG ACGGTATTCA CAGGATCACC GTCAAGGACC CCCAGTCCGG CCGTAATGCC 
AGCACGGGCG CCACAGATCA CTTCCTCCTT CGTGTCGGTT CATCCAACAA CCCGCTCGTT 
AACCCCATGA AGGCCAATTA CTCGAAGACG CTGGTGACGA AAGAGGGTAG TGATCTCTTC 
GTCAACCACG CCGCGGCCGG TGCCGATAAG TGGCGCTATT CAACCAACTG GGGCTCGACT 
TGGAGCGACT GGCTCGAGTA TAAGGGAGGC AAGGAGAAGA TCGATAAGCT AGCTTGGAAT 
GGGACCAAGA GACAGGAATG GGACGGTGAC CATGTCATGG TTCAGTACTG GTCCAAGCTA 
CTTGGTAGCG CCTCATTCGT CCAGTCTGGT GACAGCAAAG AGACCAAGAC GCGCCGCTTC 
CCCCACCTCT TCGCCCATGG TCTCTTTAAC AAGTTCGGCT TCGATGCTGG TGTCAAGAAT 
GAGCTGAAGC TGGTTGGTGA CGGCTTGTGG GAGGGCCACG TGATGGATGA GTGGCCCACG 
CACTTCCAGC TCAACGTATG GGGCATGAAT CCCGATCAGA GGCCTGACGC CGGGTGGGTA 
TTTGGCGATG TGGATGGAGA TGGGGTTCTG GATCGTATGC CGCCTTCATC TTTGGCACTA 
AACGTGATCA ATGCTACGGA ACCGCCACCG ATGCCTGCGC TGTCTTGGAA GCTTGTCTTC 
AACGATGCAT TGCTCACTTT CCACAAGGAA CCCCAGGGAA ACGTGGCGGT TCAGATCATT 
ATGTTTATCC TACTGGCTTC ACTCCCCATC GTCGGCGGTC TCACTGCCGT CTGGACTTTC 
ATGGGCTCCT TTTACAAGGT CAAGGTTAAC AAGGTTGGCT TCAAGCGCCG CGGTCGATCA 
CCACTCCGCG ATATTGGCAA GCGCATCAGC AGTACCGTTT CTTTCGAGAA CTTCCGTCAT 
AAGGACGTGG GCGATACAGA GCTCGCTGTC ATCCCAGGCA AGCGCAGAAA GGTCATCATA 
GCCACGATGG AATACAATAT CGACGACTGG AACATTAAGA TCAAGATTGG CGGTCTCGGC 
GTCATGGCCC AGCTCATGGG TAAGGCACTG GAGCACCAGG ATCTGATCTG GGTTGTGCCC 
TGCGTCGGTG GCATCGACTA CCCGATCGAC CAGCGCGCTG AGCCTATGTA CGTGCCCATC 
ATGGGCAAGG AGTACGAAAT CGAAGTGCAG TACCACCAGG TTCAAAATAT CACGTACGTA 
CTGCTCGACG CACCCATCTT CCGCACCCAA TCCAAGGCCG ACCCCTACCC GCCACGCATG 
GACGACATGG ACTCGGCCAT CTACTATGCT GCATGGAACT ACTGTATTGC TGAAGCCATT 
CGTCGGTTCA ACCCGGACCT GTACCACATC AACGACTATC ACGGTGCGGC AGCGCCATTG 
TATTTGCTTC CTGAACGCAC CATTCCATGC GCTCTTTCGC TTCACAATGC CGAATTTCAA 
GGCATGTGGC CTATGCGCAC GCCTGAGGAG TCCAAGGAGG TGTGCGAGGT GTTCAACTTG 
GATCCGGAAA TCGTGAAGGA GTATGTCCAA TTCGGATCCG TCTTCAACCT CCTTCATGCC 
GGAGCAAGCT ATTTGCGCGT TCACCAAAAG GGCTTTGGTG CCGTGGGCGT CTCGAAGAAG 
TATGGCGACC GCTCTTACGC CCGCTATCCC ATCTTCTGGG GTCTGTCCAA GATTGGCCAG 
CTGCCAAACC CTGACCCGAG TGACACGGCC GAGTGGAGCA GAGACGAGCA AATCCAGGCG 
AAGGACGTCG TCGTCGATCT TGAGGCTGAG GCAAAACGCG GTGATTTGAG GAGGCAAGCG 
CAGGAGTGGG CTGGGTTAGA AGTCAACCCG GATGCGGAGC TCTTCGTCTT CGTCGGCAGA 
TGGTCGCTCC AGAAAGGTGT CGATCTCATT GCTGATATCT TCCCGTCCAT CCTGGAAAAG 
TATCCCACCA CCCAGCTCAT TTGCGTCGGC CCGGTTATCG ATCTGTATGG AAAGTTCGCG 
GCACTCAAGT TGGCGAAGCT TATGGAGAAG TATCCCAAGC GCGTTTACAG CAAGCCCGAG 
TTTACCGCCC TGCCTCCTTG TATCTTCAGC GGCGCTGAGT TTGCCCTGAT TCCCTCCCGC 
GACGAACCGT TTGGTCTGGT TGCTGTTGAA TTCGGTCGGA AGGGCGCGCT CGGCGTGGGT 
GCCCGCGTCG GTGGCCTTGG ACAGATGCCT GGTTTCTGGT ACACGATCGA GTCGACCGCT 
CCTCAGCATC TTATTCAGCA ATTCCGTGGA GCCATTGTCA GTGCTTTGGA AAGCAAGAAA 
AAGGACCGTC AGATGATGAG AGCCTGGAGT GCCAAGCAAC GTTTCCCTGT TGCTCAATGG 
GTTGAGGACC TCGACACGCT TCAGACCGAG GCTATTCGCA TTCATCACAA GGAAGCCAAG 
AAGCGGAAGA GAGTTCCTAG TGGCTCTTTG CTCACAGTTC CTTCTACCGT TGATCTTCAC 
GGCAACAATA CCCAGCGTGA TTACTTTGAT GATGCGGTCT CGACCCCTGC GGGCGCACGA 
TCCCGAACGG CATCTACTGT TTCTACTCCG GGGCCTCAAG ACGGTGCAAC ACATCACAGA 
CGAACCCTCT CCAGCCCCTT TGATGATGAC ATTCCCAGCA TTGCGTCACC ACCCCCCGGG 
ACTCCCGGAT CTCCTCCAAC CGTTAACGTG GATCGTGTCG GCGACCATTC AGACCACAAT 
GGTGAAGATG GTGCTGATGA ACCCCTCATG CCACCGAACC CCTTGTTCCT TAACCCCTCT 
GCCAGTTCGT CGGTCACAGA TGTTGCCTCT ATTGCAGCTA ATGTCTACGG CCACGCGGGT 
GACCGCAGTA GTGTCGACAC CTTTGCGATG CGCATGATGT CTCCCGACAG TTCCGAAATT 
CGTCCTCAAG CTTTTGGTCT GCTCAATCCA CAAGCCCGTC CCGGTGCTGA AGCCCTCTAC 
CAAGCACGCA ACCGCAACAG CTCGCGTCTC TCCGTAATTG ATGTCGTTGG TGACAGGCAG 
GATTTCCGCC TGCAAAAGGT TGATCCTTTC TTTACTGATG CCAATGGTCA ATACTACCGC 
GAATTCGAGC GAAAACTCAA CGGGCTCACG GCTAAGAACT CGGAAACCGA ACTCTGCATC 
GAGGATTACC TCAAGGAAAG CGAGAAGGAG TGGTTCAAGG AATTCAGAAA CGCCAAACTG 
GGCCGCAGCC GCAGTCCGAG CCGCTCCCGG AGTCCCATGC CAGGCTTGAA GATCAAGAAG 
ACGAGACACG CGAGCATCGG CAGCGTCCAG AGTATTTCGC CTACCGAAGA CGACGATCGT 
GAGAACGAGG AACGCAACAG CAGCAACAAT GAGGGTGTCG GTCACCATCG CGACGACCAA 
TTTCTGCTTG GCGACGGGTA CAAGCCGCCC ACTGGCTTAA AGAAGCTGCT CTCCATCCGC 
CTTGGCGACT GGCCCATTTA CTCTTTCATC ATTGCACTCG GCCAGATTCT TGCCGCCAAC 
ACATATCAGA TCGTACTACT CGCTGGTGAA TCCGGACAAA CGCCTACACA ACTTTATATC 
GTTGCGGGCA CCTATGCCCT CGGCTCCCTT CTGTGGTGGT TCATGTTCCG CCGCCTGCCG 
GCCCTGTACT CGCTCTCCCT GCCCTGGCTC TTCTATGGCC TGGCATTCAT GCTGCTCGGC 
GTAACGCCGT TTATGCCTGA CCACGTCCGT TTCCCAGTCC AAAACACTGC GTCGGCATTG 
TACGCCGCTG GTGCATCAAG TGGATCGTTG TTCTTTGCAA TGAACTTTGG CGATGAAGGC 
GGTGTTCCCA TAATGACCTG GATTTTCCGC GCTGCCATCA TCCAAGGAAT CCAACAAGTC 
TACATTCTGG CCCTCTGGTA CTGGGGTAGC CTCCTCACCG CGCAAACCCC CACCGGCTCT 
CCGCACGTCG GCACCTGGAT CGCCAACAAG GTCCCCGTCG CCCTCGTTAT CACCGTTCCC 
ATCGCCCTGA TTTTCTGGTG CCTAGGCGTC ATCTCCTTCG TCGGTCTACC TGATTACTAC 
CGCCAACTCC CCGACAAGAT CCCCTCCTTC TACAAGTCAC TCTTGCGCCG CCACATCGTG 
CCCTGGTTTT TGTTGATGGT CGTCATCCAG AACTACTTCC TCTCGGCCCC CTACGGCCGC 
ACCTGGGAGT TCTTGTTTTA TTCCCGCGCC GTTCCCGGCT GGGCCATCTT GCTTCTGGCC 
ATCGGCTTCT TCGTCGGCGT TTGGTGTCTG CTGCTCTGGC TCTTTGCCTA CTTCACCAAA 
ACCCACCCCT GGATCGTCCC CCTCTTTGCC ATCGGCCTCG GCGCCCCGCG CTGGGCACAG 
ATGTTGTGGG CCACCAGTGG AATCGGGCTC TATCTCCCCT GGTGCGGCAC TGTGGTGCTT 
TCGGCGATCA TCTCCCGCTG CTTGTGGCTG TGGTTAGGGC TGCTGGATAC CGTGCAAGGA 
GTGGGGTTGG GTATGGTTTT GCTGCTGACG CTCACGAGGC AGCATGTGGC TGCCACGCTT 
ATTGGGGCGC AGTTTTTGGG GGCCGTGTTC ATGATGCTGG CGAGGGCTAC GGCGCCGGAT 
AAGGATGGAC CGGGAGATGT TTTCCCGGAT TTTAGTGCGG GCGTGATGCC CGGGTTGGGA 
AGGCCGTGGT TTTGGGTTGT CTTGGGGTTG CAGCTTGTGC TGCCGATTGG GTTTTTCAAG 
TTCTTCAGGA AGGAGCAGGT TGCCAAGCCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU02478 cDNA ORF clone, Neurospora crassa OR74A

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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