NCU05250 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU05250
Entrez Gene ID	3877935
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_956694.2	XP_961787.1	hypothetical protein

Pathways from BioSystems

Homologies

Neurospora crassa	NCU05250	XP_961787.1
Magnaporthe oryzae (rice blast fungus)	MGG_01726	XP_003714723.1
Schizosaccharomyces pombe (fission yeast)	mis4	NP_592917.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU05250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU05250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf03465	XM_956694.2 Latest version!	Neurospora crassa OR74A hypothetical protein (NCU05250), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU05250 ( XM_956694.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_961787.1 Neurospora crassa OR74A hypothetical protein (NCU05250), mRNA.

Price & Availability↑

CloneID	ONf03465
Clone ID Related Accession (Same CDS sequence)	XM_956694.2
Accession Version	XM_956694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_956694.1.

ATGGCATACC AGCATCCATA CCACCACAGC ATGGATCCCA ACGCCCAAGA CTACCATGGC 
TCCCAAGGCG CGCCGGCGTC CTCATCGACG CAACAAGCTG TGGCATTTAC GAGACCTTTT 
GCCCTCTACG AAGCTCTGCC CTATACGCCT TTCACCTCGA TCGTGCCTTT CGACCCAAGT 
ATCTTGCCGA CACCCTCGAT CGGAGCTGCG TCGCCCGCCC CCAGCTTGAT CGACCTCCTC 
AACACTCAGG ACTTCGATGG CCTCAATCAA GAACTTCACA ACACAGGTCC AAGCAAACGC 
GTTCAGCACA CACTCAGTCA AGTCCAGCAC TTGATAGAGA GGGCGAATAT CACAGAGTTC 
AAATTCAAGA TCGGGCCGAA AGCACCAACC AGCGGTAGTT CCTCTACTGC TGCCCCAATA 
CCCTCGTTGG CTTCGACACT ATCTCCATTC TCAAAGATGG TTTACGAGCA AACGTCAATA 
CCGTTCAGGT ATCCAACGCC AGATACCCCC GGCATGACCT CTACTACAAA TGGCCAGCAA 
CAAAAGATCG AGCGGACTAC GCCCGTCCTC GTCAAACAAG AGTCACCGGT TCCTTCACCG 
AAAGTCGAGC ATAGAGCTGA GAGACATACT CCAACCAACA CCCCAAAGAA CCGCCAAGCG 
TACTCGCAGA ACCGGGCCCG GTTTGAAATA GTGTTACCTA CCAGGGCCGA ACTGGAGAGG 
GCCCAGGCGG CTCTTCAAGC CACTTCGAGC CAGGGAATCG CGTCAAGTGA ACCACCAGTC 
TCAACTCGGC CATCCATTTC TCAGCCTCCG CCTCAGTTTG CACCCATACA GCCGGCACCC 
CTTCAACCAG CACCACAGCA GCCTACGCTC TCGGCGCCTC CCGAGTTAGC TGCACCCGTG 
CCTCCGAAAC GGCCTAGTGA AGAGGTGGCT GCTCCTGCTG CGCCGACTGC TATTGTTAGT 
CCGACAAAAT CGACCTCTCA CCCCCCCTCC AACTCCCAAC GACCAGCTGT TGCTATTGCT 
ATTGAGCTGC CTCATGCGCC TGCTTTTAAC AAGAGTGAGT ATATGGCTGT TCCCGATGAG 
CCTGACGAGC CGGTCAATCT TTCGGAGCGG AAACGGAAAC GAGAAGACCT AGACGAGGAG 
CTATATGGGG AGAGCCTGGG TTTCCAGCAG CGAAGCAACG CTGCCCTTCA TGAGCTGAGA 
ATTTTCCTGC AACAAGTTTT CGAGGCGGAG AATTACGCTG TTCATAATCG TACATCAAAC 
GAATGGGTGC TGCTAACTAT TGATGGAGAA AGCACGCTAT CTCCAGTGTG CCAGACCAAG 
GCCCAAACGC TATTGGCAAA GGTCATTTCG CTGAACTGTT TTCACAAAGT TCCGCTCCAA 
GATCTTCTTC GCTTACTACG ATTGTGCGAA AACGCCTTGA AGCAAGCCGA CTCTCTAGAG 
ATCAAGGTTG AGGAGTCATG GGCTCCCGCG GATGTTGATT CGTGGATCCA GCAGCTTCCT 
GCGTTAGAAA CAGCTCTCAA AGCTGCCAGG ACAGCACTAC GCATGATGTG TGGAGGGCGT 
GAGGATAAAC AGCTATACTC GGAGGATACA ATCGATCAGT CACTTAATCT GTTCAAGAGG 
GTAATGGACG GTATTGTGAT TCCGGTTGCC GAGTTGAGGA GTTCCGATAA CACAGCAGAG 
CTCTTCAAGT TACTTTCTTC CCACAAAAAG AGGATCGCAT CACTCTTCAA CGACAGCCAA 
AAACTGTTTT CGCAGATGTC AGTACTTTTG GCCAAGATTG ACACATCAGA TACGGTCACC 
AACACCCTGG AGTTTACGGC TTCACAATTG ATTTTCCTCG AGACCGCTCA CGCAGAAAGG 
GAGTCGGTTC TTGACACTCA AAAGTTTGAT GGCTTCCGTC TTGTGGCTAT GGATATGTTA 
TCGCAGATCT TCCATTTGAA TCCAGCCCAA CGGCAAGGCA TCTTTGACGA GATTCTGACA 
TCGCTAGAAA AACTGCCACT GGGAAAGCGT GCTCGGACGT TCAAGCTGGT TGATGGAAGT 
AGCATACAGC CGGTTTCTGC ATTGATCATG CGGTTGGTCC AAACCAGCGC AGGACAAGTT 
GATGAGACCA AGACACACGG AGGCAAAGCT CTTCGCTCAG ATGGTGATGA TGAAGAGGAC 
GAACTGGCAG GCGCAGTGAC CAGTGGGAAG CAGCTGTCCT TTGTCATTCG TGATGAGGAC 
CACGGCGCAA TCCAGCATTC CACTGCAATC CAGGAGCTTA CCGCTCTTGC AACGCCCTTG 
ATGGACACGG CGAAGCGGAA TGCTTCTTAT GTTGTCAACT TCATTGTGAA CCGTGCGCTG 
ACGTCCACCA AATCTGGCGA TACTCCATAT CGTAACCTTC TCGATCTTTT CGTGGAAGAC 
TTCACAACCT GCTTGGACAA CCCAGATTGG CCGGCAGCCG AGCTTCTGCT TCGTCTGCTG 
AACTTCATGA TGGTTAAGCT TGTGGAGGGT GATAAGACAA GCGTTACCGC CAAGAACATG 
GCCCTTGAAC TGATGGGCAC CATGGGCGCT GCGATCTCCA AGCTTCGCAG TCATGTCCGG 
AAGACGGCGA ATGCGTTGGA TGCTAGGGAT GCGGATGAAC TGGGTCTTTT CCTGTCAGAT 
CTTGCTGCCT CAGCACTGGA ACTGCGATCA CGTCCGGAAA CAATGGTTGC TTGGGTTGGT 
CCATACCGGG CGACCTTGGA ATGGCTCGAA AGCCGCTTCA CCGAAGATCC GCATCTGGCA 
AGCGCTATCA GCTTCATCAT CTCGGATTGG GGTACAAAGG TTTGTGTCGG GTATGATTCT 
TGCGAAGATG ATGACCGCGA GCGCGATCAA GAGTTTGGCA AATTGGCCTA CAGATTGCGA 
CAGATGATCC AGGACCGCCG ATGGCTCTCG AACGAATACT CTTTTAAGAA AGTATCACCT 
AACCAGGCCA AATTGTCGTA TTCCGTCACG TTGTTGCGGT CGCAATTATG CGAATCCTTC 
GGCGCGATTC TCAACATCTT GCTCGGTTCG ATGGCTAGCG ACCAGCCGAC AGTCAGGAGC 
AAGAGTTTAA AGAGCATCAA TCAGGTCCTG GAGACGGACC CGTCTATTCT CGATGGTGAT 
TCTGTGGTGG TCCAGCTTAT TCTGCGCTGC TCAAACGATT CGTCAACCCA AGTTCGAGAC 
TCGGCGCTCG GCCTTATCGG CAAATGCATC AGTATGAGGC CAGAGTTGGA GACCAAGATG 
ATCGGAACTG TGGTGGATCG CTTCATCGAC GCTGGACCAG GAGTCAGGAA GCGGGCTATG 
AAACTCGCCA AGGACATCTA CCTTCGAAAC AAGAGCAAGG CGATCCGTAG CGCTATTGCA 
AACGGTCTGC TTCACCGTGT GCAGGACCCA GAGGAGAGCG TGAGGGACCT GGCTCGCCAG 
GTTATCGAAG AGATATGGTT CATCCCCTTT CAGGAGGGCG AGAACTCTGC ATCATCTCAG 
ATCTCCTTCT CGGATCATGT TGCCTTGATG GTTCAGACGG TCAAGCAAGG AAACGTGACC 
AATGTCTTGG ACAAAGTCTT GCAGACTCTC CTGGCCCCGG ATTCGAAATG TGCGGCGGCG 
TCGTTGGAAG TCTGCAGAAA GCTGGTTGCA AGCATGTTTG AGCTGGTGGA CAATCCTGAG 
TCGGGAGATT CGTCTGTTCC TTCGGGTCGC GATGCTCTAA CCGTCCTTCA GATTTTTGCA 
AAAGCTGATC CGAGCCTTTT CACGTTTGAG CAACTTCGAC TTCTCAAGCC TCATATCGAC 
AGCATGAACA GCAGCGAAGA TTTGGTTGTG TCGCGGGCAG TCGTTGTCAT CTACAAACTA 
GTTCTCCCAC AAGTCTCAAG CGTGCACAAC GAATTCCTTA CGGATGCTCG CAACAAACTC 
TTGCCTGCTG TTCCAAAGGT TACAAGGGTC TTGCTCGATG ATATCGTGGC ATGTCTCTGG 
ATCATTAGCG AACGGTTAAA CAACTCGGAC AATCTCGCAA GACTCGTGTG CTCCAGTTTG 
GTGGCCATAA ACCAGATGCG CGTCCAGAAT CCGAACAAGC CCCTGGATGG AAAGCCGAGA 
CTGCAATTTG AACGCTACTC GCTGATCGTG GGTATGATCG GCAAGCATTG CAATCTTGAT 
AGCCATCTTG AGCTTTTCAA GCAGAAGCTT GGTCTTAAGA TGACAGGGTC TTCGATATCA 
AAGCTTATGG TCGATATCGT GGTACCATTT GCGTCCCATA TTCAACCTTT GGATGTGAGG 
AAAGCGGCAC TCGACTCGGT TGGTCTGGTG TGCCAGTCCT CGCCTAGGAA TTATGTGGCT 
GCCAATGTCT ACACAACATT CCAGCAAGTC TTTGACGACC AAAATCCAGC GCTGGAATCG 
ATGGTCTTGC GTTCTTTCCG GGAGTTCCTC TTTACGGAGG AGAAGAGATC TGAACAGGCT 
CCGGAAACTG CGGCTGGTGA TGGCAAAGGC AAGAAGAAAC GCGAGCTGAC TGTGATTGGA 
GGCACAAACT ACGATGATGT GGCCAGTGCG ACCACTCATC GGTTCCTCAA GGAGATCATT 
CGTATTGCCA CTGCGACTCA GGACGAGCAC GCTTTCTTGG CAGTTGAGGT CCTTGCCAGC 
ATTAACCGTC AAGGTCTGGT TCATCCCAAG GAAACGGGCG TGACCTTCAT TACCCTAGAG 
ACTTCGTCTA ACCCCCGTAT CTCTGAGCTC GCTTTTCTGG AGCACAAGTC ACTTCATGGA 
AAGCATGAAA CCGTTGTTGA ACGCGAATAC GTCAAGGCCA TACAGTCGGC CTTCGCGTAC 
CAGCGCGATA TTGTCAAGGA TCCGCGTGGC GCGACAGTCA ACCCATTCAC TCCCAAGCTG 
CATCTTATGA TGGAGGTGCT CAAGATTAGC AAGTCGAAGA ATCGTCAAAA GTTCCTGGAG 
AAGCTCTGCA CTCAGGTGGA CTTTGATGTG TCGAAACTCG AACTAACCGG CGAGGAACTC 
CCTCCTCATG TGCAATATGC CCGCTTCATC ATCGAGAACA TGGCCTTCTT CGAGTACATG 
ACGGTAGGCG AGCTCCAATC CACCGTTTAC ACGATGGAGA AGCTAGTCAC ATCAACCGGG 
TCAACTATTG CGCAGGTCAT TGAGTCGGAG GTTTTCCAAG TCCGCATGGA CGCTATTGAC 
GGTCCCGCGC CTGAACCTGA GCCCACACCA GCACCTGCGC CCACATTTGG AGAGCAGCCT 
AACGAACAAC CCATGACGTC TGTTCAGCCT GTCCAACCAA CACTCCCCGA TATCGACATT 
CAGCGCCTCC GCCAGTTGAC AGCAGGGTCC ATGATCCTCC TCGCCCTGTG GGAAGTCCGC 
ACTTACCTCC GCCGGCTATA TAACATGGGC ACCAATCGCC GCGAGCAGAA GGCCAAGATT 
CAGGGCAAAG ACCTCTCTCG GCCACCAGTC AAGGTCCAGG GCGTCACCGG CGACAAGGTC 
TGGGAGGAGA TTGGTACTGT CATGACGGGT CTCATTTCGC GCGAGCGCAT GATGCAAACG 
TGCAAAGCGT TCGTGGAGCT TATGAATGTC GACAAGGAGT TCCTCGTTCA GGATGAAGAT 
GAGGACGAAG ATATGATGGA CCCCTCGACG CCGCCGAGCG GGGGTGAAGA GGATGACTAC 
GATGAGGCAG GCCCAGCCGC CGGTCCCGGG AGGGGGAGAA AGAGGAAATC AAGTGCCACG 
GGTCCCAATG GATCACCCGC TCCGAAGAGG AGAGGAAGGC CACCAAGAAG CAATTCCAAG 
GGGCCACAGG GGCCGCAAGG GCCGCCGAAG AAACGCGGCA GACCTAGAAA GAATCCGTTG 
CCCGAGGCGA GCCAACAACA TGACGAAATG GAGGCAGACT GGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_961787.1
CDS	556..6423
Translation	MAYQHPYHHSMDPNAQDYHGSQGAPASSSTQQAVAFTRPFALYEALPYTPFTSIVPFDPSILPTPSIGAASPAPSLIDLLNTQDFDGLNQELHNTGPSKRVQHTLSQVQHLIERANITEFKFKIGPKAPTSGSSSTAAPIPSLASTLSPFSKMVYEQTSIPFRYPTPDTPGMTSTTNGQQQKIERTTPVLVKQESPVPSPKVEHRAERHTPTNTPKNRQAYSQNRARFEIVLPTRAELERAQAALQATSSQGIASSEPPVSTRPSISQPPPQFAPIQPAPLQPAPQQPTLSAPPELAAPVPPKRPSEEVAAPAAPTAIVSPTKSTSHPPSNSQRPAVAIAIELPHAPAFNKSEYMAVPDEPDEPVNLSERKRKREDLDEELYGESLGFQQRSNAALHELRIFLQQVFEAENYAVHNRTSNEWVLLTIDGESTLSPVCQTKAQTLLAKVISLNCFHKVPLQDLLRLLRLCENALKQADSLEIKVEESWAPADVDSWIQQLPALETALKAARTALRMMCGGREDKQLYSEDTIDQSLNLFKRVMDGIVIPVAELRSSDNTAELFKLLSSHKKRIASLFNDSQKLFSQMSVLLAKIDTSDTVTNTLEFTASQLIFLETAHAERESVLDTQKFDGFRLVAMDMLSQIFHLNPAQRQGIFDEILTSLEKLPLGKRARTFKLVDGSSIQPVSALIMRLVQTSAGQVDETKTHGGKALRSDGDDEEDELAGAVTSGKQLSFVIRDEDHGAIQHSTAIQELTALATPLMDTAKRNASYVVNFIVNRALTSTKSGDTPYRNLLDLFVEDFTTCLDNPDWPAAELLLRLLNFMMVKLVEGDKTSVTAKNMALELMGTMGAAISKLRSHVRKTANALDARDADELGLFLSDLAASALELRSRPETMVAWVGPYRATLEWLESRFTEDPHLASAISFIISDWGTKVCVGYDSCEDDDRERDQEFGKLAYRLRQMIQDRRWLSNEYSFKKVSPNQAKLSYSVTLLRSQLCESFGAILNILLGSMASDQPTVRSKSLKSINQVLETDPSILDGDSVVVQLILRCSNDSSTQVRDSALGLIGKCISMRPELETKMIGTVVDRFIDAGPGVRKRAMKLAKDIYLRNKSKAIRSAIANGLLHRVQDPEESVRDLARQVIEEIWFIPFQEGENSASSQISFSDHVALMVQTVKQGNVTNVLDKVLQTLLAPDSKCAAASLEVCRKLVASMFELVDNPESGDSSVPSGRDALTVLQIFAKADPSLFTFEQLRLLKPHIDSMNSSEDLVVSRAVVVIYKLVLPQVSSVHNEFLTDARNKLLPAVPKVTRVLLDDIVACLWIISERLNNSDNLARLVCSSLVAINQMRVQNPNKPLDGKPRLQFERYSLIVGMIGKHCNLDSHLELFKQKLGLKMTGSSISKLMVDIVVPFASHIQPLDVRKAALDSVGLVCQSSPRNYVAANVYTTFQQVFDDQNPALESMVLRSFREFLFTEEKRSEQAPETAAGDGKGKKKRELTVIGGTNYDDVASATTHRFLKEIIRIATATQDEHAFLAVEVLASINRQGLVHPKETGVTFITLETSSNPRISELAFLEHKSLHGKHETVVEREYVKAIQSAFAYQRDIVKDPRGATVNPFTPKLHLMMEVLKISKSKNRQKFLEKLCTQVDFDVSKLELTGEELPPHVQYARFIIENMAFFEYMTVGELQSTVYTMEKLVTSTGSTIAQVIESEVFQVRMDAIDGPAPEPEPTPAPAPTFGEQPNEQPMTSVQPVQPTLPDIDIQRLRQLTAGSMILLALWEVRTYLRRLYNMGTNRREQKAKIQGKDLSRPPVKVQGVTGDKVWEEIGTVMTGLISRERMMQTCKAFVELMNVDKEFLVQDEDEDEDMMDPSTPPSGGEEDDYDEAGPAAGPGRGRKRKSSATGPNGSPAPKRRGRPPRSNSKGPQGPQGPPKKRGRPRKNPLPEASQQHDEMEADWL

Target ORF information:

RefSeq Version	XM_956694.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A hypothetical protein (NCU05250), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_956694.2

ORF Insert Sequence:

ATGGCATACC AGCATCCATA CCACCACAGC ATGGATCCCA ACGCCCAAGA CTACCATGGC 
TCCCAAGGCG CGCCGGCGTC CTCATCGACG CAACAAGCTG TGGCATTTAC GAGACCTTTT 
GCCCTCTACG AAGCTCTGCC CTATACGCCT TTCACCTCGA TCGTGCCTTT CGACCCAAGT 
ATCTTGCCGA CACCCTCGAT CGGAGCTGCG TCGCCCGCCC CCAGCTTGAT CGACCTCCTC 
AACACTCAGG ACTTCGATGG CCTCAATCAA GAACTTCACA ACACAGGTCC AAGCAAACGC 
GTTCAGCACA CACTCAGTCA AGTCCAGCAC TTGATAGAGA GGGCGAATAT CACAGAGTTC 
AAATTCAAGA TCGGGCCGAA AGCACCAACC AGCGGTAGTT CCTCTACTGC TGCCCCAATA 
CCCTCGTTGG CTTCGACACT ATCTCCATTC TCAAAGATGG TTTACGAGCA AACGTCAATA 
CCGTTCAGGT ATCCAACGCC AGATACCCCC GGCATGACCT CTACTACAAA TGGCCAGCAA 
CAAAAGATCG AGCGGACTAC GCCCGTCCTC GTCAAACAAG AGTCACCGGT TCCTTCACCG 
AAAGTCGAGC ATAGAGCTGA GAGACATACT CCAACCAACA CCCCAAAGAA CCGCCAAGCG 
TACTCGCAGA ACCGGGCCCG GTTTGAAATA GTGTTACCTA CCAGGGCCGA ACTGGAGAGG 
GCCCAGGCGG CTCTTCAAGC CACTTCGAGC CAGGGAATCG CGTCAAGTGA ACCACCAGTC 
TCAACTCGGC CATCCATTTC TCAGCCTCCG CCTCAGTTTG CACCCATACA GCCGGCACCC 
CTTCAACCAG CACCACAGCA GCCTACGCTC TCGGCGCCTC CCGAGTTAGC TGCACCCGTG 
CCTCCGAAAC GGCCTAGTGA AGAGGTGGCT GCTCCTGCTG CGCCGACTGC TATTGTTAGT 
CCGACAAAAT CGACCTCTCA CCCCCCCTCC AACTCCCAAC GACCAGCTGT TGCTATTGCT 
ATTGAGCTGC CTCATGCGCC TGCTTTTAAC AAGAGTGAGT ATATGGCTGT TCCCGATGAG 
CCTGACGAGC CGGTCAATCT TTCGGAGCGG AAACGGAAAC GAGAAGACCT AGACGAGGAG 
CTATATGGGG AGAGCCTGGG TTTCCAGCAG CGAAGCAACG CTGCCCTTCA TGAGCTGAGA 
ATTTTCCTGC AACAAGTTTT CGAGGCGGAG AATTACGCTG TTCATAATCG TACATCAAAC 
GAATGGGTGC TGCTAACTAT TGATGGAGAA AGCACGCTAT CTCCAGTGTG CCAGACCAAG 
GCCCAAACGC TATTGGCAAA GGTCATTTCG CTGAACTGTT TTCACAAAGT TCCGCTCCAA 
GATCTTCTTC GCTTACTACG ATTGTGCGAA AACGCCTTGA AGCAAGCCGA CTCTCTAGAG 
ATCAAGGTTG AGGAGTCATG GGCTCCCGCG GATGTTGATT CGTGGATCCA GCAGCTTCCT 
GCGTTAGAAA CAGCTCTCAA AGCTGCCAGG ACAGCACTAC GCATGATGTG TGGAGGGCGT 
GAGGATAAAC AGCTATACTC GGAGGATACA ATCGATCAGT CACTTAATCT GTTCAAGAGG 
GTAATGGACG GTATTGTGAT TCCGGTTGCC GAGTTGAGGA GTTCCGATAA CACAGCAGAG 
CTCTTCAAGT TACTTTCTTC CCACAAAAAG AGGATCGCAT CACTCTTCAA CGACAGCCAA 
AAACTGTTTT CGCAGATGTC AGTACTTTTG GCCAAGATTG ACACATCAGA TACGGTCACC 
AACACCCTGG AGTTTACGGC TTCACAATTG ATTTTCCTCG AGACCGCTCA CGCAGAAAGG 
GAGTCGGTTC TTGACACTCA AAAGTTTGAT GGCTTCCGTC TTGTGGCTAT GGATATGTTA 
TCGCAGATCT TCCATTTGAA TCCAGCCCAA CGGCAAGGCA TCTTTGACGA GATTCTGACA 
TCGCTAGAAA AACTGCCACT GGGAAAGCGT GCTCGGACGT TCAAGCTGGT TGATGGAAGT 
AGCATACAGC CGGTTTCTGC ATTGATCATG CGGTTGGTCC AAACCAGCGC AGGACAAGTT 
GATGAGACCA AGACACACGG AGGCAAAGCT CTTCGCTCAG ATGGTGATGA TGAAGAGGAC 
GAACTGGCAG GCGCAGTGAC CAGTGGGAAG CAGCTGTCCT TTGTCATTCG TGATGAGGAC 
CACGGCGCAA TCCAGCATTC CACTGCAATC CAGGAGCTTA CCGCTCTTGC AACGCCCTTG 
ATGGACACGG CGAAGCGGAA TGCTTCTTAT GTTGTCAACT TCATTGTGAA CCGTGCGCTG 
ACGTCCACCA AATCTGGCGA TACTCCATAT CGTAACCTTC TCGATCTTTT CGTGGAAGAC 
TTCACAACCT GCTTGGACAA CCCAGATTGG CCGGCAGCCG AGCTTCTGCT TCGTCTGCTG 
AACTTCATGA TGGTTAAGCT TGTGGAGGGT GATAAGACAA GCGTTACCGC CAAGAACATG 
GCCCTTGAAC TGATGGGCAC CATGGGCGCT GCGATCTCCA AGCTTCGCAG TCATGTCCGG 
AAGACGGCGA ATGCGTTGGA TGCTAGGGAT GCGGATGAAC TGGGTCTTTT CCTGTCAGAT 
CTTGCTGCCT CAGCACTGGA ACTGCGATCA CGTCCGGAAA CAATGGTTGC TTGGGTTGGT 
CCATACCGGG CGACCTTGGA ATGGCTCGAA AGCCGCTTCA CCGAAGATCC GCATCTGGCA 
AGCGCTATCA GCTTCATCAT CTCGGATTGG GGTACAAAGG TTTGTGTCGG GTATGATTCT 
TGCGAAGATG ATGACCGCGA GCGCGATCAA GAGTTTGGCA AATTGGCCTA CAGATTGCGA 
CAGATGATCC AGGACCGCCG ATGGCTCTCG AACGAATACT CTTTTAAGAA AGTATCACCT 
AACCAGGCCA AATTGTCGTA TTCCGTCACG TTGTTGCGGT CGCAATTATG CGAATCCTTC 
GGCGCGATTC TCAACATCTT GCTCGGTTCG ATGGCTAGCG ACCAGCCGAC AGTCAGGAGC 
AAGAGTTTAA AGAGCATCAA TCAGGTCCTG GAGACGGACC CGTCTATTCT CGATGGTGAT 
TCTGTGGTGG TCCAGCTTAT TCTGCGCTGC TCAAACGATT CGTCAACCCA AGTTCGAGAC 
TCGGCGCTCG GCCTTATCGG CAAATGCATC AGTATGAGGC CAGAGTTGGA GACCAAGATG 
ATCGGAACTG TGGTGGATCG CTTCATCGAC GCTGGACCAG GAGTCAGGAA GCGGGCTATG 
AAACTCGCCA AGGACATCTA CCTTCGAAAC AAGAGCAAGG CGATCCGTAG CGCTATTGCA 
AACGGTCTGC TTCACCGTGT GCAGGACCCA GAGGAGAGCG TGAGGGACCT GGCTCGCCAG 
GTTATCGAAG AGATATGGTT CATCCCCTTT CAGGAGGGCG AGAACTCTGC ATCATCTCAG 
ATCTCCTTCT CGGATCATGT TGCCTTGATG GTTCAGACGG TCAAGCAAGG AAACGTGACC 
AATGTCTTGG ACAAAGTCTT GCAGACTCTC CTGGCCCCGG ATTCGAAATG TGCGGCGGCG 
TCGTTGGAAG TCTGCAGAAA GCTGGTTGCA AGCATGTTTG AGCTGGTGGA CAATCCTGAG 
TCGGGAGATT CGTCTGTTCC TTCGGGTCGC GATGCTCTAA CCGTCCTTCA GATTTTTGCA 
AAAGCTGATC CGAGCCTTTT CACGTTTGAG CAACTTCGAC TTCTCAAGCC TCATATCGAC 
AGCATGAACA GCAGCGAAGA TTTGGTTGTG TCGCGGGCAG TCGTTGTCAT CTACAAACTA 
GTTCTCCCAC AAGTCTCAAG CGTGCACAAC GAATTCCTTA CGGATGCTCG CAACAAACTC 
TTGCCTGCTG TTCCAAAGGT TACAAGGGTC TTGCTCGATG ATATCGTGGC ATGTCTCTGG 
ATCATTAGCG AACGGTTAAA CAACTCGGAC AATCTCGCAA GACTCGTGTG CTCCAGTTTG 
GTGGCCATAA ACCAGATGCG CGTCCAGAAT CCGAACAAGC CCCTGGATGG AAAGCCGAGA 
CTGCAATTTG AACGCTACTC GCTGATCGTG GGTATGATCG GCAAGCATTG CAATCTTGAT 
AGCCATCTTG AGCTTTTCAA GCAGAAGCTT GGTCTTAAGA TGACAGGGTC TTCGATATCA 
AAGCTTATGG TCGATATCGT GGTACCATTT GCGTCCCATA TTCAACCTTT GGATGTGAGG 
AAAGCGGCAC TCGACTCGGT TGGTCTGGTG TGCCAGTCCT CGCCTAGGAA TTATGTGGCT 
GCCAATGTCT ACACAACATT CCAGCAAGTC TTTGACGACC AAAATCCAGC GCTGGAATCG 
ATGGTCTTGC GTTCTTTCCG GGAGTTCCTC TTTACGGAGG AGAAGAGATC TGAACAGGCT 
CCGGAAACTG CGGCTGGTGA TGGCAAAGGC AAGAAGAAAC GCGAGCTGAC TGTGATTGGA 
GGCACAAACT ACGATGATGT GGCCAGTGCG ACCACTCATC GGTTCCTCAA GGAGATCATT 
CGTATTGCCA CTGCGACTCA GGACGAGCAC GCTTTCTTGG CAGTTGAGGT CCTTGCCAGC 
ATTAACCGTC AAGGTCTGGT TCATCCCAAG GAAACGGGCG TGACCTTCAT TACCCTAGAG 
ACTTCGTCTA ACCCCCGTAT CTCTGAGCTC GCTTTTCTGG AGCACAAGTC ACTTCATGGA 
AAGCATGAAA CCGTTGTTGA ACGCGAATAC GTCAAGGCCA TACAGTCGGC CTTCGCGTAC 
CAGCGCGATA TTGTCAAGGA TCCGCGTGGC GCGACAGTCA ACCCATTCAC TCCCAAGCTG 
CATCTTATGA TGGAGGTGCT CAAGATTAGC AAGTCGAAGA ATCGTCAAAA GTTCCTGGAG 
AAGCTCTGCA CTCAGGTGGA CTTTGATGTG TCGAAACTCG AACTAACCGG CGAGGAACTC 
CCTCCTCATG TGCAATATGC CCGCTTCATC ATCGAGAACA TGGCCTTCTT CGAGTACATG 
ACGGTAGGCG AGCTCCAATC CACCGTTTAC ACGATGGAGA AGCTAGTCAC ATCAACCGGG 
TCAACTATTG CGCAGGTCAT TGAGTCGGAG GTTTTCCAAG TCCGCATGGA CGCTATTGAC 
GGTCCCGCGC CTGAACCTGA GCCCACACCA GCACCTGCGC CCACATTTGG AGAGCAGCCT 
AACGAACAAC CCATGACGTC TGTTCAGCCT GTCCAACCAA CACTCCCCGA TATCGACATT 
CAGCGCCTCC GCCAGTTGAC AGCAGGGTCC ATGATCCTCC TCGCCCTGTG GGAAGTCCGC 
ACTTACCTCC GCCGGCTATA TAACATGGGC ACCAATCGCC GCGAGCAGAA GGCCAAGATT 
CAGGGCAAAG ACCTCTCTCG GCCACCAGTC AAGGTCCAGG GCGTCACCGG CGACAAGGTC 
TGGGAGGAGA TTGGTACTGT CATGACGGGT CTCATTTCGC GCGAGCGCAT GATGCAAACG 
TGCAAAGCGT TCGTGGAGCT TATGAATGTC GACAAGGAGT TCCTCGTTCA GGATGAAGAT 
GAGGACGAAG ATATGATGGA CCCCTCGACG CCGCCGAGCG GGGGTGAAGA GGATGACTAC 
GATGAGGCAG GCCCAGCCGC CGGTCCCGGG AGGGGGAGAA AGAGGAAATC AAGTGCCACG 
GGTCCCAATG GATCACCCGC TCCGAAGAGG AGAGGAAGGC CACCAAGAAG CAATTCCAAG 
GGGCCACAGG GGCCGCAAGG GCCGCCGAAG AAACGCGGCA GACCTAGAAA GAATCCGTTG 
CCCGAGGCGA GCCAACAACA TGACGAAATG GAGGCAGACT GGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU05250 cDNA ORF clone, Neurospora crassa OR74A

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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