Gene Symbol | npd-1 |
Entrez Gene ID | 3873678 |
Full Name | nitropropane dioxygenase-1 |
Synonyms | ncd-2 |
Gene Type | protein-coding |
Organism | Neurospora crassa OR74A |
ORF » Species Summary » Neurospora crassa OR74A » npd-1 cDNA ORF clone
Gene Symbol | npd-1 |
Entrez Gene ID | 3873678 |
Full Name | nitropropane dioxygenase-1 |
Synonyms | ncd-2 |
Gene Type | protein-coding |
Organism | Neurospora crassa OR74A |
mRNA | Protein | Name |
---|---|---|
XM_952495.3 | XP_957588.1 | FMN-dependent 2-nitropropane dioxygenase |
The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The pH dependence of the kinetic isotope affects the nitroethane as substrate for the 2-NPD.
Title: Inflated kinetic isotope effects in the branched mechanism of Neurospora crassa 2-nitropropane dioxygenase.
Mutagenesis studies suggest that this group is histidine 196 as evident from the inability of a H196N variant form of the enzyme to catalyze the formation of ethylnitronate from nitroethane
Title: The nonoxidative conversion of nitroethane to ethylnitronate in Neurospora crassa 2-nitropropane dioxygenase is catalyzed by histidine 196.
The enzyme was more specific for nitronates than nitroalkanes, as suggested by the second order rate constant k(cat)/K(m) determined with 2-nitropropane and primary nitroalkanes with alkyl chain lengths between 2 and 6 carbons
Title: Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzed by 2-nitropropane dioxygenase.
The following npd-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npd-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | ONf04535 | |
Clone ID Related Accession (Same CDS sequence) | XM_952495.3 | |
Accession Version | XM_952495.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-10-10 | |
Organism | Neurospora crassa OR74A | |
Product | FMN-dependent 2-nitropropane dioxygenase | |
Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_952495.2. |
1 | ATGCACTTCC CAGGCCACAG CAGCAAGAAG GAGGAATCTG CCCAAGCGGC CCTCACGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_957588.1 |
CDS | 361..1497 |
Translation |
Target ORF information:
Target ORF information:
|
XM_952495.3 |
1 | ATGCACTTCC CAGGCCACAG CAGCAAGAAG GAGGAATCTG CCCAAGCGGC CCTCACGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The genome sequence of the filamentous fungus Neurospora crassa. |