NCU00480 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU00480
Entrez Gene ID	3872312
Full Name	ubiquitin C-terminal hydrolase
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_951072.3	XP_956165.3	ubiquitin C-terminal hydrolase

Pathways from BioSystems

Homologies

Neurospora crassa	NCU00480	XP_956165.2
Magnaporthe oryzae (rice blast fungus)	MGG_05666	XP_003710563.1
Schizosaccharomyces pombe (fission yeast)	ubp12	NP_588530.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU00480 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00480 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf09943	XM_951072.3 Latest version!	Neurospora crassa OR74A ubiquitin C-terminal hydrolase (NCU00480), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU00480 ( XM_951072.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956165.3 Neurospora crassa OR74A ubiquitin C-terminal hydrolase (NCU00480), mRNA.

Price & Availability↑

CloneID	ONf09943
Clone ID Related Accession (Same CDS sequence)	XM_951072.3
Accession Version	XM_951072.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	ubiquitin C-terminal hydrolase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_951072.2.

ATGGGTGGCG CTTCAGAGCA GACACATCGA TCTGGTTCTC CCCTGAAACG AAGAGCATCC 
AGTATGGATC CAGAGTTTGA GAACGCGGAC TCGCGGGAGG ACGTGGAAAT GCTCACGGTT 
CCTGATACCC AGCCGCAAGA CCAACAGCCT GGGCAAGATG TGGAAGAGCC TCAGCCCGTG 
GAAGCAGACG CCGTCGGAGG TACCAAGGTG CCCATTGCTG ATATGGAACT GCCCTTGAAG 
ACAGAAATCC CACCGATCGA GCAACAGATC AAGACGATCG AAACACTCCT CAAGGCCTTC 
AATGATAGGG GCGCAAAAGA AGGAGATGTG GCATACTTAG TATCACGGCA GTGGCTGAAC 
AAGGCCCAGG CGTTCGGTGC TAATGGGAAG CCTACAAGCA AGGAGCCTTC AGACGAGACT 
ATTGGGCCAA TTGATAACTC GGATATCATT CAAGCTATCT TCACCGACTC CGCCGGCCAG 
CAGTGCGTGA AGCTAAAGCC GGGCGTGGGA CCAGAGTCTG CTGAGCTGTT TCCCAAGGAC 
GCATGGGACC TGGTGCTGTC ATGGTATGGT CTGGCGTCTG GACAGGCCCC TATTGTGCGG 
ATAGCGCACA ATACCGCCCT TGATGCTGCC AGCGAGCCCA ACGTTCAGTT TGAGTTCCAC 
CCCCCCGTTT TTACCGTTCA CAGGCTATGG TCGGCAGCGA GTCCTCTGCC TATCGAACAG 
GAGATTAAGC TGAAGAAGCC GCCACCGCCC GTCATTGTCC AAAGCGCATC TGCCTCATTT 
CACCAATTTC TTAAGCAAAT CAAGAAGCTG ACTGGTGTTC CTATGGATCG TAAAGTCCGG 
GTCTGGCAGC GGTTGCAAAC AATTCCTGCC ACAGAGGAGG CCTCGAAGCC CTCTGGTATG 
GAGACCCCTC CAGATTCCCC AGGGCGTTCC TCTAGCATAC CCAACTTCCT ACCTAGGGCC 
CCTGGCTCCT GGCCGGAAAT GCTCGTGGAT GTTGAAACTT TCAGCCAACT AGAGCGTGGT 
GTGGAGCGCG ATCACCTCGC AGCAGAGGAT ACCACAACCA ATCCCAACTA CAACGGGAGG 
AAGAGTCTGT CATTGGTTGG CCTTACTGTG GACCAAACCT TGATCATCGA CGAACAGGTT 
GACCGAGATG ATTACGTCTC TACATTCTTC ACTAAAATGA CCAATGGCAA CAAGACCGTC 
GGCAACGGCA TTGTCGCTCA GGCCCGTGGT ACTGTCAGCG GACGTAACAG CCCGGCTCCC 
CCGAGTGTCC TCAATAGGGG CCGGGTGCAA CCCAGATCCG GTCGTACGCC AGGGTGCGTT 
GGCTTGCAGA ACCTGGGCAA TACATGCTAC ATGAATTCGG CTCTGCAATG TCTACGGAGC 
GTTGAAGAGT TGACCAAGTA TTTCCTCACT CACGAGGCCA AGAAAGAGAT CAACCCTGAC 
AATCCGCTTT CCCATAACGG AGACGTTGCT ATGGCATACA TGCGGCTGTT GGATGAAATA 
TACAAGAATC CTGCGCCCAA CTCGGTCGCG CCCCGTCACT TCAAAGGCAT CGTGGGCCGA 
TATGCCCCCG CCTTCTCTGG CTATGGGCAG CAGGACTCCC AAGAATTCTT GGGTTTCTTG 
CTCGATGGCC TACAAGAGGA CCTGAACCGC ATCAAGAAGA AGCCGTACAT TGAGAAGCCA 
GACTCTACAG ATGACATGAT CGGTAACCCT GCTGCTATCA AAGAAATGGC CGAGAAGGTT 
TGGGATATCA CAAAGAAGCG TGACGACTCG ATCATTGCCG ACCTGTTTAC CGGATTATAC 
AAGTCAACCC TTCATTGCCC TGTGTGCGAC AAGATCAGCA TCACATTCGA TCCCTTCAAC 
AACCTCACTT TGCCCTTGCC AATGAGCAAC GTCTGGTCCC GGACTGTCAA GTATTATCCC 
TTAAACGAAC CCCCAGTGGA AATCGTTGTA GAGGTCGATA AGACGAGCGC TTTCAAGGCC 
ATAAAGGAGT TCATTTCGAC GAGAGTCGGT GTCCCCCCGG AGCGTCTTGT TGGAGGTGAA 
GAGTTCAAGG GTAAATTCTT CAAGCAGTAC GACGACATGA TGGCGATCAA CGAAGAGATT 
CAGCCGGGTG ACGTTGCAGT GATACATGAA GTGGAAGCGC CGCCTACCAA TGTCAACGCA 
AAGAAGGCCA AGAAGCAGGT ATACAGGTCG CTGCTGGACG ATGCCGAGGA AGAGCCCGCT 
CAACCCACGT CGAATCCCCT GGCGGAACGT CTCCTTGTTC CAGTTCTGCA TCGTACTATG 
GACCCTAATG GGGCCGGTGT CAGATATCAT AGGAAAGTAG AGGACATCCC ACCGCCACAT 
TACATTGTGT TGACGCCTCA GGAGGCTTGT GATGAGAATA TCATCCGCAG AAAAATCCTG 
CAAAAGGTGG CCACCTTGTC TACGTGGTCG GAATTCACTG CCGACGAAGA GGCATCAGAA 
GCTACGGATC CCGAGATGGT CAATGGCGCA TCGGATATTG ACTCTGCAGA CTCTAAGGTA 
GTTGCCAAGT CCGTCGAGGG CGAGGAAGAT ATTGTCGACG TGACGATGGG CAACACTGGT 
TCTGCCAAAC CAACAGCGTC GCCAGCTCCT CAAACTCTTC CGGCGCTCCT CAAGCGTTTC 
AAGTCGCAGC AGCCTAAGTG GTTAGACCCT TCAGTTTTTC TGAATCCAGC CCTGCAGAAC 
CTCTTTGCGA TGTCTTACTT TAGGGAGTTG CATGGCGGTG TTCCTACAGG GTGGCAAAGC 
GTCAACGACA CCAGCAAACA CGAAAGACTG AGCTCGAGGG CCCCTAAGAC GGATTCGTCG 
GATGTGGAGA TGCGGAGCCC GAATGCATTG GATGGCTCGG ATGACAGCGG TAGCGAAGAA 
GCTTCTTCGG AACAGGTAGA TTCCGTGACA CGGATGGCCG ATGAGTCGAG CGAGGGCTCG 
GATGCCCCGA AAGGAAGTGA TCGATTCATC GCTAGTCCTC CTAGGGGAGC AAATGCCGTT 
CCACGAGGCA AAAACAAGAA GATGAAAGCT GGCCGCACTT ATGGCAAGAA GGCTAAGAAG 
CGTTTTGAGA AAGAACAGCT GAGAAAACGG GCTGCGGCCG CCCAGCAACC CATCGAGGTC 
CCTGAGCAAG ATTACCGTGT CGACTCCGAG GAAGGGCCCC TTGTGCGTCT TGGCGAAGGC 
ATCGTTGTGG ACTGGTACGA GGACGCTTTT GATACTGTCT TCTCCCCTGC GAACAGGACC 
TACAACAAGA TTGAGACTCT AAATGACCCG TCGTTGGGTG CCAAGAAGAG GCAACGGGAG 
CTGCGTAAGA AGCACGGTAT CAGTCTAGAT GATTGTTTGG CCGAGTTCGA GAAGGAAGAA 
ATCTTGTCCG AGCAAGATAC ATGGTATTGT CCCCGTTGCA AGGAGCACCG TCGCGCAAGC 
AAGAAATTCG ACCTTTGGAA GACGCCAGAT ATTCTAGTCG TCCATCTGAA ACGCTTTAGC 
TCCGTTGGCT GGCGTCGGGA TAAGCTGGAT GTCCTTGTCG ACTTCCCCAT CGAGGGCCTC 
GACCTCACCG AGCGGGTCAT TGACAAGGAA GACGGTAAAC AGGAGATCTA TGACCTGATT 
GCTGTGGATG ACCATTGGGG CGGCTTGGGC GGTGGCCATT ACACTGCCTT TGCCAAGAAT 
TTTGTTGATG ATCAGTGGTA TGAGTTCAAT GATTCATCCG TTTCGAAGGT GACCGATACC 
TCCAAGGTTG TCTCACCCGC TGCCTACTTG CTGTTCTACC GTCGCCGCTC AGACAAACCT 
CTAGGCGGGC CCAAATGCGA GGAAGTCGCC CAGCGCTATT CCCTTGACGA CGATGAGGAA 
ATGCTGGACT CGGGGGAAGG CCAGCGTCTC GGTCATGGCT CCTCCCTTCG TGGGTCGCCG 
AGCGCTTCGA ACGGAGCAGG TCAAACTCTC CTGCGAGGAG AAGTTGGTTC GGTTAGTGGC 
CGCGGTCCAA GTGGCGACTC CGAACTGCCC TCCTATCAGG AAACCAATGG CACGATTACT 
GGTCCTGAGG TGCGGAAGAG CATCGAGTTT GAGGACGAGG GCATTGATCT ACCCAACTAC 
GAAGCCGCGG GCAACATGTC CGGCGTGGCC TCTGCTCTCC CCACAACCTG GAGCTTTGAC 
AACATTCCCA AGACCGGCTC CGAAGCGAGT GGCTTGGACG ACGAGATTGC GAGTGATGTG 
GCACAAGGCG ACAACTCCGG GGACGATGCC TCGGTCTTTG CCGGTATCAA TGAATATGGC 
TCCGCTGTAG ATTTCATCGG ACCTGCTGCA CAGGACGACG ATTACTCGGC ATTCACTGAC 
AATGTCCCGC CGCCGCCGTC AGTGCAGGAT CAGGACACCA TGGACCAAAT CGCGCAGATG 
ACATGGGAAC ACAAGGAGCA AGTTCATGAG GTGTTCGTCA ATGGCGGCGC TGACATTGAC 
GACGATAAGG TTGCGGAGAT TCACGTCGAC GATCACGAGC AGCAAGGACA ATCACCAGCA 
GTTCGGGAAC CGCCAAAGGA GACGGAAGGA GGCGATAAGT CGTCAGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956165.3
CDS	1212..5762
Translation	MGGASEQTHRSGSPLKRRASSMDPEFENADSREDVEMLTVPDTQPQDQQPGQDVEEPQPVEADAVGGTKVPIADMELPLKTEIPPIEQQIKTIETLLKAFNDRGAKEGDVAYLVSRQWLNKAQAFGANGKPTSKEPSDETIGPIDNSDIIQAIFTDSAGQQCVKLKPGVGPESAELFPKDAWDLVLSWYGLASGQAPIVRIAHNTALDAASEPNVQFEFHPPVFTVHRLWSAASPLPIEQEIKLKKPPPPVIVQSASASFHQFLKQIKKLTGVPMDRKVRVWQRLQTIPATEEASKPSGMETPPDSPGRSSSIPNFLPRAPGSWPEMLVDVETFSQLERGVERDHLAAEDTTTNPNYNGRKSLSLVGLTVDQTLIIDEQVDRDDYVSTFFTKMTNGNKTVGNGIVAQARGTVSGRNSPAPPSVLNRGRVQPRSGRTPGCVGLQNLGNTCYMNSALQCLRSVEELTKYFLTHEAKKEINPDNPLSHNGDVAMAYMRLLDEIYKNPAPNSVAPRHFKGIVGRYAPAFSGYGQQDSQEFLGFLLDGLQEDLNRIKKKPYIEKPDSTDDMIGNPAAIKEMAEKVWDITKKRDDSIIADLFTGLYKSTLHCPVCDKISITFDPFNNLTLPLPMSNVWSRTVKYYPLNEPPVEIVVEVDKTSAFKAIKEFISTRVGVPPERLVGGEEFKGKFFKQYDDMMAINEEIQPGDVAVIHEVEAPPTNVNAKKAKKQVYRSLLDDAEEEPAQPTSNPLAERLLVPVLHRTMDPNGAGVRYHRKVEDIPPPHYIVLTPQEACDENIIRRKILQKVATLSTWSEFTADEEASEATDPEMVNGASDIDSADSKVVAKSVEGEEDIVDVTMGNTGSAKPTASPAPQTLPALLKRFKSQQPKWLDPSVFLNPALQNLFAMSYFRELHGGVPTGWQSVNDTSKHERLSSRAPKTDSSDVEMRSPNALDGSDDSGSEEASSEQVDSVTRMADESSEGSDAPKGSDRFIASPPRGANAVPRGKNKKMKAGRTYGKKAKKRFEKEQLRKRAAAAQQPIEVPEQDYRVDSEEGPLVRLGEGIVVDWYEDAFDTVFSPANRTYNKIETLNDPSLGAKKRQRELRKKHGISLDDCLAEFEKEEILSEQDTWYCPRCKEHRRASKKFDLWKTPDILVVHLKRFSSVGWRRDKLDVLVDFPIEGLDLTERVIDKEDGKQEIYDLIAVDDHWGGLGGGHYTAFAKNFVDDQWYEFNDSSVSKVTDTSKVVSPAAYLLFYRRRSDKPLGGPKCEEVAQRYSLDDDEEMLDSGEGQRLGHGSSLRGSPSASNGAGQTLLRGEVGSVSGRGPSGDSELPSYQETNGTITGPEVRKSIEFEDEGIDLPNYEAAGNMSGVASALPTTWSFDNIPKTGSEASGLDDEIASDVAQGDNSGDDASVFAGINEYGSAVDFIGPAAQDDDYSAFTDNVPPPPSVQDQDTMDQIAQMTWEHKEQVHEVFVNGGADIDDDKVAEIHVDDHEQQGQSPAVREPPKETEGGDKSSD

Target ORF information:

RefSeq Version	XM_951072.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A ubiquitin C-terminal hydrolase (NCU00480), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_951072.3

ORF Insert Sequence:

ATGGGTGGCG CTTCAGAGCA GACACATCGA TCTGGTTCTC CCCTGAAACG AAGAGCATCC 
AGTATGGATC CAGAGTTTGA GAACGCGGAC TCGCGGGAGG ACGTGGAAAT GCTCACGGTT 
CCTGATACCC AGCCGCAAGA CCAACAGCCT GGGCAAGATG TGGAAGAGCC TCAGCCCGTG 
GAAGCAGACG CCGTCGGAGG TACCAAGGTG CCCATTGCTG ATATGGAACT GCCCTTGAAG 
ACAGAAATCC CACCGATCGA GCAACAGATC AAGACGATCG AAACACTCCT CAAGGCCTTC 
AATGATAGGG GCGCAAAAGA AGGAGATGTG GCATACTTAG TATCACGGCA GTGGCTGAAC 
AAGGCCCAGG CGTTCGGTGC TAATGGGAAG CCTACAAGCA AGGAGCCTTC AGACGAGACT 
ATTGGGCCAA TTGATAACTC GGATATCATT CAAGCTATCT TCACCGACTC CGCCGGCCAG 
CAGTGCGTGA AGCTAAAGCC GGGCGTGGGA CCAGAGTCTG CTGAGCTGTT TCCCAAGGAC 
GCATGGGACC TGGTGCTGTC ATGGTATGGT CTGGCGTCTG GACAGGCCCC TATTGTGCGG 
ATAGCGCACA ATACCGCCCT TGATGCTGCC AGCGAGCCCA ACGTTCAGTT TGAGTTCCAC 
CCCCCCGTTT TTACCGTTCA CAGGCTATGG TCGGCAGCGA GTCCTCTGCC TATCGAACAG 
GAGATTAAGC TGAAGAAGCC GCCACCGCCC GTCATTGTCC AAAGCGCATC TGCCTCATTT 
CACCAATTTC TTAAGCAAAT CAAGAAGCTG ACTGGTGTTC CTATGGATCG TAAAGTCCGG 
GTCTGGCAGC GGTTGCAAAC AATTCCTGCC ACAGAGGAGG CCTCGAAGCC CTCTGGTATG 
GAGACCCCTC CAGATTCCCC AGGGCGTTCC TCTAGCATAC CCAACTTCCT ACCTAGGGCC 
CCTGGCTCCT GGCCGGAAAT GCTCGTGGAT GTTGAAACTT TCAGCCAACT AGAGCGTGGT 
GTGGAGCGCG ATCACCTCGC AGCAGAGGAT ACCACAACCA ATCCCAACTA CAACGGGAGG 
AAGAGTCTGT CATTGGTTGG CCTTACTGTG GACCAAACCT TGATCATCGA CGAACAGGTT 
GACCGAGATG ATTACGTCTC TACATTCTTC ACTAAAATGA CCAATGGCAA CAAGACCGTC 
GGCAACGGCA TTGTCGCTCA GGCCCGTGGT ACTGTCAGCG GACGTAACAG CCCGGCTCCC 
CCGAGTGTCC TCAATAGGGG CCGGGTGCAA CCCAGATCCG GTCGTACGCC AGGGTGCGTT 
GGCTTGCAGA ACCTGGGCAA TACATGCTAC ATGAATTCGG CTCTGCAATG TCTACGGAGC 
GTTGAAGAGT TGACCAAGTA TTTCCTCACT CACGAGGCCA AGAAAGAGAT CAACCCTGAC 
AATCCGCTTT CCCATAACGG AGACGTTGCT ATGGCATACA TGCGGCTGTT GGATGAAATA 
TACAAGAATC CTGCGCCCAA CTCGGTCGCG CCCCGTCACT TCAAAGGCAT CGTGGGCCGA 
TATGCCCCCG CCTTCTCTGG CTATGGGCAG CAGGACTCCC AAGAATTCTT GGGTTTCTTG 
CTCGATGGCC TACAAGAGGA CCTGAACCGC ATCAAGAAGA AGCCGTACAT TGAGAAGCCA 
GACTCTACAG ATGACATGAT CGGTAACCCT GCTGCTATCA AAGAAATGGC CGAGAAGGTT 
TGGGATATCA CAAAGAAGCG TGACGACTCG ATCATTGCCG ACCTGTTTAC CGGATTATAC 
AAGTCAACCC TTCATTGCCC TGTGTGCGAC AAGATCAGCA TCACATTCGA TCCCTTCAAC 
AACCTCACTT TGCCCTTGCC AATGAGCAAC GTCTGGTCCC GGACTGTCAA GTATTATCCC 
TTAAACGAAC CCCCAGTGGA AATCGTTGTA GAGGTCGATA AGACGAGCGC TTTCAAGGCC 
ATAAAGGAGT TCATTTCGAC GAGAGTCGGT GTCCCCCCGG AGCGTCTTGT TGGAGGTGAA 
GAGTTCAAGG GTAAATTCTT CAAGCAGTAC GACGACATGA TGGCGATCAA CGAAGAGATT 
CAGCCGGGTG ACGTTGCAGT GATACATGAA GTGGAAGCGC CGCCTACCAA TGTCAACGCA 
AAGAAGGCCA AGAAGCAGGT ATACAGGTCG CTGCTGGACG ATGCCGAGGA AGAGCCCGCT 
CAACCCACGT CGAATCCCCT GGCGGAACGT CTCCTTGTTC CAGTTCTGCA TCGTACTATG 
GACCCTAATG GGGCCGGTGT CAGATATCAT AGGAAAGTAG AGGACATCCC ACCGCCACAT 
TACATTGTGT TGACGCCTCA GGAGGCTTGT GATGAGAATA TCATCCGCAG AAAAATCCTG 
CAAAAGGTGG CCACCTTGTC TACGTGGTCG GAATTCACTG CCGACGAAGA GGCATCAGAA 
GCTACGGATC CCGAGATGGT CAATGGCGCA TCGGATATTG ACTCTGCAGA CTCTAAGGTA 
GTTGCCAAGT CCGTCGAGGG CGAGGAAGAT ATTGTCGACG TGACGATGGG CAACACTGGT 
TCTGCCAAAC CAACAGCGTC GCCAGCTCCT CAAACTCTTC CGGCGCTCCT CAAGCGTTTC 
AAGTCGCAGC AGCCTAAGTG GTTAGACCCT TCAGTTTTTC TGAATCCAGC CCTGCAGAAC 
CTCTTTGCGA TGTCTTACTT TAGGGAGTTG CATGGCGGTG TTCCTACAGG GTGGCAAAGC 
GTCAACGACA CCAGCAAACA CGAAAGACTG AGCTCGAGGG CCCCTAAGAC GGATTCGTCG 
GATGTGGAGA TGCGGAGCCC GAATGCATTG GATGGCTCGG ATGACAGCGG TAGCGAAGAA 
GCTTCTTCGG AACAGGTAGA TTCCGTGACA CGGATGGCCG ATGAGTCGAG CGAGGGCTCG 
GATGCCCCGA AAGGAAGTGA TCGATTCATC GCTAGTCCTC CTAGGGGAGC AAATGCCGTT 
CCACGAGGCA AAAACAAGAA GATGAAAGCT GGCCGCACTT ATGGCAAGAA GGCTAAGAAG 
CGTTTTGAGA AAGAACAGCT GAGAAAACGG GCTGCGGCCG CCCAGCAACC CATCGAGGTC 
CCTGAGCAAG ATTACCGTGT CGACTCCGAG GAAGGGCCCC TTGTGCGTCT TGGCGAAGGC 
ATCGTTGTGG ACTGGTACGA GGACGCTTTT GATACTGTCT TCTCCCCTGC GAACAGGACC 
TACAACAAGA TTGAGACTCT AAATGACCCG TCGTTGGGTG CCAAGAAGAG GCAACGGGAG 
CTGCGTAAGA AGCACGGTAT CAGTCTAGAT GATTGTTTGG CCGAGTTCGA GAAGGAAGAA 
ATCTTGTCCG AGCAAGATAC ATGGTATTGT CCCCGTTGCA AGGAGCACCG TCGCGCAAGC 
AAGAAATTCG ACCTTTGGAA GACGCCAGAT ATTCTAGTCG TCCATCTGAA ACGCTTTAGC 
TCCGTTGGCT GGCGTCGGGA TAAGCTGGAT GTCCTTGTCG ACTTCCCCAT CGAGGGCCTC 
GACCTCACCG AGCGGGTCAT TGACAAGGAA GACGGTAAAC AGGAGATCTA TGACCTGATT 
GCTGTGGATG ACCATTGGGG CGGCTTGGGC GGTGGCCATT ACACTGCCTT TGCCAAGAAT 
TTTGTTGATG ATCAGTGGTA TGAGTTCAAT GATTCATCCG TTTCGAAGGT GACCGATACC 
TCCAAGGTTG TCTCACCCGC TGCCTACTTG CTGTTCTACC GTCGCCGCTC AGACAAACCT 
CTAGGCGGGC CCAAATGCGA GGAAGTCGCC CAGCGCTATT CCCTTGACGA CGATGAGGAA 
ATGCTGGACT CGGGGGAAGG CCAGCGTCTC GGTCATGGCT CCTCCCTTCG TGGGTCGCCG 
AGCGCTTCGA ACGGAGCAGG TCAAACTCTC CTGCGAGGAG AAGTTGGTTC GGTTAGTGGC 
CGCGGTCCAA GTGGCGACTC CGAACTGCCC TCCTATCAGG AAACCAATGG CACGATTACT 
GGTCCTGAGG TGCGGAAGAG CATCGAGTTT GAGGACGAGG GCATTGATCT ACCCAACTAC 
GAAGCCGCGG GCAACATGTC CGGCGTGGCC TCTGCTCTCC CCACAACCTG GAGCTTTGAC 
AACATTCCCA AGACCGGCTC CGAAGCGAGT GGCTTGGACG ACGAGATTGC GAGTGATGTG 
GCACAAGGCG ACAACTCCGG GGACGATGCC TCGGTCTTTG CCGGTATCAA TGAATATGGC 
TCCGCTGTAG ATTTCATCGG ACCTGCTGCA CAGGACGACG ATTACTCGGC ATTCACTGAC 
AATGTCCCGC CGCCGCCGTC AGTGCAGGAT CAGGACACCA TGGACCAAAT CGCGCAGATG 
ACATGGGAAC ACAAGGAGCA AGTTCATGAG GTGTTCGTCA ATGGCGGCGC TGACATTGAC 
GACGATAAGG TTGCGGAGAT TCACGTCGAC GATCACGAGC AGCAAGGACA ATCACCAGCA 
GTTCGGGAAC CGCCAAAGGA GACGGAAGGA GGCGATAAGT CGTCAGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU00480 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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