NCU03578 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03578
Entrez Gene ID	3872167
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_950924.2	XP_956017.2	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03578 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03578 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf09890	XM_950924.2 Latest version!	Neurospora crassa OR74A hypothetical protein (NCU03578), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03578 ( XM_950924.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956017.2 Neurospora crassa OR74A hypothetical protein (NCU03578), mRNA.

Price & Availability↑

CloneID	ONf09890
Clone ID Related Accession (Same CDS sequence)	XM_950924.2
Accession Version	XM_950924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). On Feb 23, 2015 this sequence version replaced XM_950924.1.

ATGCCGCCCG CTGGCTCAGA GAGGGACCAC CATGGGCACC ACCAGCTCCC CCGAGATTTA 
AGACACTCGC ATTCCGCCTC GTTTGATAAT CATGGCCGTG CCATGATCCC AATGTGGGAT 
AGCTCAGACC CCGAGAGGGC ACCACCGCCG CTTCCACTCA ACCCTCAGTC TCCCGGTGTC 
TCTAGAGCTG GCACCTCGAG TGCGATCCAG TCTGCCCACG CTGCCTTGCA GGAAAAGGCC 
CGTGAGAGTG CTAACCTGAT ACCACACATC CCCAAGCGCG CCGCTGGTGA CCCTTCTCCA 
GAGAGGACAG AGAGGCCCCC TTTGCGCAAC ACGGCTACGG CTGCGTCACA TCGCCGTCTA 
AACTCCCTTC AGAACAGCAC TGTCAAGGAT ATTAGTTTGA TGCTTGAGAG TGGGTACGTC 
TCCAATACAT CAGCCTCACG ATCACCAGAG AAGCACGAGT TTAAGCACGA TTTGAAGCAC 
GACTTCAAAT TCGATAGCAA ACTTGACACC AAGTTTGACA AGTACGAAAA GCACGAAAAC 
AACAAACACG ATAAGTACGA GAGCAAGTAC GAGAGCATAT ACGAGAGGCC ATCCACCCCT 
ACGAGGAGTC GTGAGAACCA CGTCGAATTC AAGGACGAAA AGGAAGACAC CCTAAAACCC 
GCCCCCACCA TGGGTTCACC ACGAACGGGT CCTAGTTTGA CCCCCGGCAG CATCCTCCGC 
CAAGCCAACA GCACCAGCAA CATGCGGCGA CCACATCAGA GCATCCTCGG CGAGAACACT 
CCTCCTCAGT CGGCCACCAT GCTCGCCCTT CAGAACATGA GTAGTGCCAG CAACCTCGGA 
ACGTCGTCGT CGGTCAGGGA CTCGGATCCC CTCGCTAACA TCACTAACGG CTCCAGCGGA 
GTGACCAAGA CGACTGTAAC ACCCCTAGGC CTCGAGGCCC TGTCGAACCA GCTCATCACC 
CTTACCAACA TTGCTTCGGC TCTTCAACGA GAAATGTCGG CGCTTAGCAG GCGCAGCAGA 
GATAATGCTA CAGACTTGCT CAGCTTGAAA GAGGCCACCA ATCAGCGCGA TGAGGACATT 
CGCAGGAGTT TGCGAGAGTT GATCACCGAG GCCAAGTCGA GGGCCGCGCA GAGGGAAGCG 
TACGGTGGGG GTCCGCCATC GCCTACGTCG CTAAAGTCGC GACCGTTCTC CCTGCCGCGA 
ATACCTTCGC CACATAGCTT TGGACCGTCG CTTGATCGAG AGTCGCTATT AAGCACACCC 
TCACTTGTGG CCGACACATC GTCAATTGCT CTTCTAGAAA AGATCCTTCG CGACATGGGA 
ACGAAAGAGG GCCAAGAATC GCTTCTTTCG AGACTTAACG AACTCGCCCG TCAGCTTTCC 
GGCTTTTCCA CGTCTGAAAA GCTAGAGGAG ATCATCCGTT TGGTCAAAGC CAATCAGCAA 
CATGCTCTAG TGCCAGCTAC TGGCGGAGCA GGGAGAGGTG GTGGTGGCGG CGGCGGCGGC 
GGCGGTCGCA ACAAGGCCTG GAGCTTTAGC GAAGACGAAG ACGGCCGAAA CTCGCGCGTA 
GGCCGATTCC TCGAAGAATC TGGAAATTCA CCAACTCAAA CCCAAAGCTC CCGTGCCACC 
GATTCCATGA ATGATGAAAT CCTCTCCGCC CTGCGCATAG TCAAGGATCA CCTGGCCCAA 
GGCGGCAGCC TTACCAGCGA CGTCAAGTCT CTCGTCAAGG AACTGCGCGG CGAGGTCCTC 
GACATGGGCC AAGAAATCGG TCGCCGACTT AACGAAGTCT CCTCCAAGTC CCCTCAAACC 
CTCCCTGTAG ACAATTCTAC AAAGGAAGCC ATGAACAAAA TTGTGTCAGA TGGCCTGGAG 
GAAATGCGCC AGAACATGGT CAACATCGTG CACGAACACC GCAAGAAGGC GCAAGAGACA 
GTCGCGGCAG CGCCTCGAAT CGACTCCATG ATCGATTACA GGGAGATCTA CAATAGCATG 
CGTGCTGCCC TCAAGGATTC ACAAGCCAAT AAGCCGCGCG TGCAGGAGCT TCGTAGGGAG 
GACGTTGTAC AAGCAGTGCA AGATGCTTTC GAAAAGTACA AGCCTGAAAT TGAGATCCAG 
CAGATCGGAT TGGAAAGGCA CGAGGTGCTG GACTGTTTGA AGGAGGGGCT AGGAATGTAT 
CTGCCAAAGG AGACGGCTTC CAAGCAAGAC GTGTTTGCTG CCATCAACGA AGGGTTCAGC 
AAGTTTGAGA CGGTCTCCCG GGACGATGTC CTCGAATCTG TCAGGGAATG TCTGGACGAG 
TTCGACTTCC CTGTGACTCC CCAGCTTTCG AAAGACGACA TTATGAGTGC GGTCAAGGAT 
GGTTTGAGCG ACCTCCCCAA GCCCCTGAAG CGTCAAGAGG TGGTGAATGC CATGGATGAG 
AGCCTCAACA AGTTCGACTT CACTGTTGAC ATGGCCGAAG CCGTGCAAGA TGGTGTACGG 
TCTCTGGATC TGCCAAAGGA CATGCAAACC GCCGTCACCA AAGCGCTGGA GACATTCGAC 
TTTTCCTCTG CCGGCCCATC AAGCGAAGAC ATGATCGATG CTGTCCAGGA AGCTATCGAA 
ACCATGGATC TTCCCTGTGA TGTCACACGC GCCGTCGTCA AAGCATTGCA AACCCCCGAG 
TTCGCACAGC ATATCGCTTC TTACCTCCCC CGCCCCGATC TGTCGCGTGT CGACCTTGCT 
GATGCCGTCC GCGAAGGATT TGATGCGCTT GATATTTCAG GAGATGTCGC CCGCGCTGTC 
AAAGAAAGTC TAAAAGAACT AGACCTCTCC GATCTCTCCG GCTCACATTC CAAAGCTCTC 
GTCCGCCACG GCACCACGCC TCCTTCCATC CTGAAGCCCG AAACTGAAGA AATCATTGAG 
CGACTGTACG AAATCCGTGA TTCGCTAAGA GATGAGTTCC GCGCCTTCTC AGAAAAAAGC 
AAACCCACCT CCCGTGGTGG TGACGCTGAG CGGACTACAC CTGGTATTAC CATGGACGAT 
TTCGAGAAAC TGAGGCATGA CATCGAAGCG TACGTCGACC GCGCCCGCAC CGACGGCAAC 
TCCGACGATT TGATCCCGCA CATGACTGAA ACATTGGAGA GATTTAGGGA GGAAGTGGCG 
GAGTTGGTCA AGAAGGCTTC TCGGAGCACC AACGATCTGC TAAGGGAGGA ACTTGAATCA 
CTCCGCGACG TCGTCAACGG CACCTTGGTC CCCGTAACGC CACTCGCGCA GCCCGGCAGC 
AATGCCGAGA TTCTCGAGGC CATTGGTGCT ATTCGTAAGG AGATGAATAC CACCTCTCAG 
CACACCGGTG AGGCGGCGGA AACGAAAATC CTCCAAGCTC TGCAAGAGGG ACTCGGCGAT 
TTGAAAAAGA GTATTGACAA GCTCTCTAAC AAAGCCGGTG ACCTCTCGGC TAATGACGAG 
ATTCTGGATG CGCTCAAGGA GGGCCTGAAG GGCGTCAGAG CTGATCTCGA AGAGCTTAGA 
GCAGGAGGCG GGTTTGATGA CGAATTCGCG CTGACGCCCT TTAGCAAGGA TGGTGATCAA 
CCTTCGAAGC AGCTTGGCCA TGGAAGCGTC GAGGAGATTA GAAGTCTTCA TGCTATGGTT 
GATAAGCTAG GGAAGAAGGT CGAGGCTATG GAAAGGGGTG CTGGCCCGTC AAAGGCTGCG 
GCGGGTGCCA GAACTGCTAC ATCCAAGTCT GAGCTAGGCG ACGTCGAGGC CTTGCTAAGG 
AAGCTGATCG ATTCCGTCGC CTCTCTTGCT GCCCGCGAAC AAAAGCAGCA GAAGATCAAC 
CTGTCACCCC TCGAAGAAAC CATTCGCTCC CTCCAGACTT CCGTTGACAC CGCGATTGGG 
GGTCTGAGAA AGTCCATTGA TGAGTGGACC AAGGCAAGAT CGTCATCAGC CGCGCCTAAG 
CAGCCCGAGC CTGAGCTGCT TCCCACCAGC GACCCTACCG ATCCGGCTAC GAAAGAAGAT 
GTCCAGGCAA TTGAGACCAT TCTAAGGAAT ACCAAGGACA GCTTGGACGA TTTCGTGAGC 
GCCCAGGGAC TGCAGAGGGA GCTAGTCAGG AAAGAGCATG TGGATGCACT TGAAGTCTTG 
TTGGTAGAGA CAAAGTCGAA CTTGGGAGAT TTGTCGAAGC AAGTGGAAGG TGTGTCTAGG 
AAGGAGGATG TTTCCGCTCT CGAGTCGCTC ATCGCCAAGA TGACGATTGC CTTCGACGAA 
ATGAGGACCC GCGACGAGAA AGAAGACTCC AACAAGCTCA CCAAAGCTGA TCTCGAGGCT 
GTTGAGACTG TTTGTCTCGA AGTCAAGTCC CTCGTTTCAT CGAGACCCGC CACCACTGAT 
GCCACCACCG ACTCTCCGAC AGCCACAGCC AGCCGCCCCG ACATGGACAG CAACTTCAAG 
ACCCTGACTG AGAAGCTTGA AGCTCAAGCC GAGGCTACTA CAAAGGCAAA CGGCGACATC 
ACCGACCGCG TGTCCGAAGT CAAATCCCTC CTCGAAGAGT TTCATGAAGT CATCAAAGCT 
AAACTCACTG ATGGAGCCAA GGGGGTGGAT TCTATTCACA AGGTTCTGGC TCCAATGAGC 
GAAATTGTCA AGAAGAACGC GACGCTGCAG GATGAGCTCA TGGAATTGTG GGGTGGCATG 
AAGGAGGGTT TCGAGGAAGG TCGCACGACT ATCAATAAGG CGAAGACTGA CATTGAGACC 
AAGATCGAGG AGCTTCAGAA GGCGCAGATC TACGAGATCA AGGAGACCGT TGCGACAAAG 
GGCCAAATGG AGGAGCTCAA GACTGCGCTG GAGCCTCTCA GCGGTACGGT CACGGAGGCT 
GTGGAAAAGA TGGAAGTCAG CTCAAAGACT GTTTTCGAAA GGGTGGATGA GCTGGTGTGC 
AAGGCCGATG AGAATCATCT CGATATCAAG GCTGAGCATG CCCTTACCCG AGAGCAGGTG 
CAGGAGATGA CTGGCAAGTT GGACGGCTTG CAGAGTCTTG TTGCCGAGCA TCAGCCTAAG 
GTCGCCGAGA ATCTCCAGGA GGTTCTTGCC CTTGTTGGTC AGCATTTTGA GCATTCCAAG 
ACGACAACGG AGGGTCTGCA GACCAAGGTC ATCAACGCCA TGCCCACTGT CAACGAGAAG 
CTCGAAAAGC TCGTTGGTCA TACCGGCGAA GCGGAGAAGG CTTTCGCTCA TCTCGAAGAT 
GTCCACAAAC AAGTCAAGAA TACCTCCGCC GAGCTCGCTG AGTTCATCGC CGCCCAAACT 
CAACGCGCCG TCGAAGAGGA TGAGAACAAG AAGAAGATAC TGCAAGAGAC CACAATTGCT 
CTTGAGCGCC AGCTGGCTGA AAAGGAAGCA ATCGAAGCTC GTTTGGCTGA GCTCAAAGAG 
GAGGAAGCCC GCGTTAAGGA GAGCATTAAT GTTACTTTGC GTGAGGAGCA GGATCAGCTC 
AAGTCCCAGT TCTTGGAAAA CCTCCAACAT GAACAAGCAC GTCTGAAGCA GATGAATGAG 
TCTCTAAAGC AGGAACAGGA AGAGCTCAAG TCTACGTTCT TGGCCAACTT GGCTGAGGAG 
GAGCATCGCC TCAAGACGCT CAATGAGTCC CTAAGAAAAG AGCACGAGGA AGCCAAGGCG 
GTGTTCATGG CCGGTCTCAA GGAGGAGGAG ACGAGGCTGA AGGAACTCAA TGCTTCTCTC 
AAGGAAGAGC ACGAGACCTT GAAGAAGACG TTCACAATTA ATCTTGAAGA GGAACGCGAC 
CGCTTGATGG AAATCAACGT CGCCCTCAAA GAAGAACAAG ACACCATGAA GAAGACCTTT 
GCTGCTAACC TCCGCGATGA GCGCGACCGT CTGATCGAGC TCAATTCCGC CCTCAAAGCC 
GAGCACGAGT CGCTCAAGCG GAGCTTCCTT GCCGTTTTGA AGGACGAAGA AGCCCGTCTG 
AAGGAGTCGA ACGATGCTTT GCGAGAAGAG CAGCAAGCTC TCAAGGATAA GTTCCTCAAG 
AATTTGCGCG AGGAGGAGTC TTTGCTCAAG GAAGTCAACG AAGGTCTGAG GCAAGAGCAG 
AAAGAACTTA GGAGCTCTTT CCTTGCCGCT TTCAAGGAGG AAGAGAGGCG TCTCAAGGAG 
ATCAACGCCA GCCTGCGGGA AGAGCAAGAG GAAATGAAGG CGATCTTCCG TGAAGAGCAG 
GAGAAGCTGA AGGTGGATCT GCTCGCTAAC CTGATGGAGG AGGAGTGTCG CCTTAAAGAA 
CGCAACGAGG CACTGCGCAA GGAGCATGAC AAACTTAAGG AGCAGTTTTT GGCTGATCTG 
AGGGCTGAGC AGCAGGGTAT CAAGCACAAC CTTGTTGAGC TTCGCGGAGA GCAAGAGGAG 
CTGGTGCGCA AGAAATCCAG ACTCACGGCG GACTTGTCCT CGCTCGACAC TGCCCTCCGC 
CTCCGAAGAG AAGAGCTCCA CGAGATGGAA ACCCGCGCCG AGACACTCGA GCGCCGTATC 
CTCGAGGGAG TCATGGATCA TTCACGCTTA CTTCTCATGG CCAAAGGCAA GCGCTTCACC 
ACGGGCGTCG GTCGGGAGTC AATGAATCGC AAGCGCGTTC CCTCGCAAAT GTCCACCCGC 
GCTCCCAGCG TCGTTTCACG CATGGAGTCA AGACAGCCCA CGTCGGCACT CAACATGGCT 
GCTATCAACG CCAAGACCAG AACCCCTAAG ATGGGCACGG GTGCGGGATC GTCGGCGTCT 
AACAGACGGA TTCTGTCTCT GAGTCAAATG GTCAGCGGAG CTCTGCCGGG CGGCAGCATC 
AAGCGCAGCA ATAGCGTTAG GAACGGGGCT GGTGGTGGTG GTGTCGCGGC GGTCAAGGCG 
GCGGTAGCTG CTCATCATGG TGATGACGGT ACTTCCGGGA CTTCGACACC TAAGGCTAAA 
GAACGGAAGG GAAGCTGGGC GACGACGAGT TCCAAGTCCA CGGCGAAGGG GTATGGTGGT 
CTATCGAAAC AGCATAGAGA TTTCTCCCTG AAGGACACGG ATGATGAGGA CGAGGAGGGG 
TATGACACCG CTACTCCACC AGTTGATGTG CGCTCCGGGT CAGATGAGGA CATGGTTCAC 
GTCGACGACG ACGCGCGCAC TGAGTCCTCT TCCCGCTACG GCGGCGACGA AGACCACAAC 
GAAACCGAAA CCGAAACTGA GGCGGACGAA ACAGCCACTC TACGTCGCTC CTCCCATGGT 
ACGACGGTCA TCACTTCCTC TTCCCGCTCC GGCCCTCGTT CGGCTCGATC CGGCCGCTCA 
AATGGCAGTC GGGATGACGA CGGAGAAAGC CAGTACAGCC AGGACCACGA TCAAGATGAA 
GGAGACGACA ACTACTCGGA CTACGAAACA GGCGACGATA GTGAAGACGA CTGGACCGAG 
AGGGGTAGTG CCATTTCGGG CATGACTGGT ACCACGGGCA CGAGCAGGAC GGGGCGCAGT 
AGTGTTAGCG CGCCGTCGAT TGCGCCGAGT GCGTCGGTCA TGAGCGTGGC GAGTTCAAAG 
ATGTCAAAGG CTAGTAAGAT GAGTAAGGAT TCGGCTATTT CGGGGATTCC TAGGGCGAGT 
CCGAAGAAGG GGAAAAAGGC TGGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956017.2
CDS	1036..8565
Translation	MPPAGSERDHHGHHQLPRDLRHSHSASFDNHGRAMIPMWDSSDPERAPPPLPLNPQSPGVSRAGTSSAIQSAHAALQEKARESANLIPHIPKRAAGDPSPERTERPPLRNTATAASHRRLNSLQNSTVKDISLMLESGYVSNTSASRSPEKHEFKHDLKHDFKFDSKLDTKFDKYEKHENNKHDKYESKYESIYERPSTPTRSRENHVEFKDEKEDTLKPAPTMGSPRTGPSLTPGSILRQANSTSNMRRPHQSILGENTPPQSATMLALQNMSSASNLGTSSSVRDSDPLANITNGSSGVTKTTVTPLGLEALSNQLITLTNIASALQREMSALSRRSRDNATDLLSLKEATNQRDEDIRRSLRELITEAKSRAAQREAYGGGPPSPTSLKSRPFSLPRIPSPHSFGPSLDRESLLSTPSLVADTSSIALLEKILRDMGTKEGQESLLSRLNELARQLSGFSTSEKLEEIIRLVKANQQHALVPATGGAGRGGGGGGGGGGRNKAWSFSEDEDGRNSRVGRFLEESGNSPTQTQSSRATDSMNDEILSALRIVKDHLAQGGSLTSDVKSLVKELRGEVLDMGQEIGRRLNEVSSKSPQTLPVDNSTKEAMNKIVSDGLEEMRQNMVNIVHEHRKKAQETVAAAPRIDSMIDYREIYNSMRAALKDSQANKPRVQELRREDVVQAVQDAFEKYKPEIEIQQIGLERHEVLDCLKEGLGMYLPKETASKQDVFAAINEGFSKFETVSRDDVLESVRECLDEFDFPVTPQLSKDDIMSAVKDGLSDLPKPLKRQEVVNAMDESLNKFDFTVDMAEAVQDGVRSLDLPKDMQTAVTKALETFDFSSAGPSSEDMIDAVQEAIETMDLPCDVTRAVVKALQTPEFAQHIASYLPRPDLSRVDLADAVREGFDALDISGDVARAVKESLKELDLSDLSGSHSKALVRHGTTPPSILKPETEEIIERLYEIRDSLRDEFRAFSEKSKPTSRGGDAERTTPGITMDDFEKLRHDIEAYVDRARTDGNSDDLIPHMTETLERFREEVAELVKKASRSTNDLLREELESLRDVVNGTLVPVTPLAQPGSNAEILEAIGAIRKEMNTTSQHTGEAAETKILQALQEGLGDLKKSIDKLSNKAGDLSANDEILDALKEGLKGVRADLEELRAGGGFDDEFALTPFSKDGDQPSKQLGHGSVEEIRSLHAMVDKLGKKVEAMERGAGPSKAAAGARTATSKSELGDVEALLRKLIDSVASLAAREQKQQKINLSPLEETIRSLQTSVDTAIGGLRKSIDEWTKARSSSAAPKQPEPELLPTSDPTDPATKEDVQAIETILRNTKDSLDDFVSAQGLQRELVRKEHVDALEVLLVETKSNLGDLSKQVEGVSRKEDVSALESLIAKMTIAFDEMRTRDEKEDSNKLTKADLEAVETVCLEVKSLVSSRPATTDATTDSPTATASRPDMDSNFKTLTEKLEAQAEATTKANGDITDRVSEVKSLLEEFHEVIKAKLTDGAKGVDSIHKVLAPMSEIVKKNATLQDELMELWGGMKEGFEEGRTTINKAKTDIETKIEELQKAQIYEIKETVATKGQMEELKTALEPLSGTVTEAVEKMEVSSKTVFERVDELVCKADENHLDIKAEHALTREQVQEMTGKLDGLQSLVAEHQPKVAENLQEVLALVGQHFEHSKTTTEGLQTKVINAMPTVNEKLEKLVGHTGEAEKAFAHLEDVHKQVKNTSAELAEFIAAQTQRAVEEDENKKKILQETTIALERQLAEKEAIEARLAELKEEEARVKESINVTLREEQDQLKSQFLENLQHEQARLKQMNESLKQEQEELKSTFLANLAEEEHRLKTLNESLRKEHEEAKAVFMAGLKEEETRLKELNASLKEEHETLKKTFTINLEEERDRLMEINVALKEEQDTMKKTFAANLRDERDRLIELNSALKAEHESLKRSFLAVLKDEEARLKESNDALREEQQALKDKFLKNLREEESLLKEVNEGLRQEQKELRSSFLAAFKEEERRLKEINASLREEQEEMKAIFREEQEKLKVDLLANLMEEECRLKERNEALRKEHDKLKEQFLADLRAEQQGIKHNLVELRGEQEELVRKKSRLTADLSSLDTALRLRREELHEMETRAETLERRILEGVMDHSRLLLMAKGKRFTTGVGRESMNRKRVPSQMSTRAPSVVSRMESRQPTSALNMAAINAKTRTPKMGTGAGSSASNRRILSLSQMVSGALPGGSIKRSNSVRNGAGGGGVAAVKAAVAAHHGDDGTSGTSTPKAKERKGSWATTSSKSTAKGYGGLSKQHRDFSLKDTDDEDEEGYDTATPPVDVRSGSDEDMVHVDDDARTESSSRYGGDEDHNETETETEADETATLRRSSHGTTVITSSSRSGPRSARSGRSNGSRDDDGESQYSQDHDQDEGDDNYSDYETGDDSEDDWTERGSAISGMTGTTGTSRTGRSSVSAPSIAPSASVMSVASSKMSKASKMSKDSAISGIPRASPKKGKKAGK

Target ORF information:

RefSeq Version	XM_950924.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A hypothetical protein (NCU03578), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_950924.2

ORF Insert Sequence:

ATGCCGCCCG CTGGCTCAGA GAGGGACCAC CATGGGCACC ACCAGCTCCC CCGAGATTTA 
AGACACTCGC ATTCCGCCTC GTTTGATAAT CATGGCCGTG CCATGATCCC AATGTGGGAT 
AGCTCAGACC CCGAGAGGGC ACCACCGCCG CTTCCACTCA ACCCTCAGTC TCCCGGTGTC 
TCTAGAGCTG GCACCTCGAG TGCGATCCAG TCTGCCCACG CTGCCTTGCA GGAAAAGGCC 
CGTGAGAGTG CTAACCTGAT ACCACACATC CCCAAGCGCG CCGCTGGTGA CCCTTCTCCA 
GAGAGGACAG AGAGGCCCCC TTTGCGCAAC ACGGCTACGG CTGCGTCACA TCGCCGTCTA 
AACTCCCTTC AGAACAGCAC TGTCAAGGAT ATTAGTTTGA TGCTTGAGAG TGGGTACGTC 
TCCAATACAT CAGCCTCACG ATCACCAGAG AAGCACGAGT TTAAGCACGA TTTGAAGCAC 
GACTTCAAAT TCGATAGCAA ACTTGACACC AAGTTTGACA AGTACGAAAA GCACGAAAAC 
AACAAACACG ATAAGTACGA GAGCAAGTAC GAGAGCATAT ACGAGAGGCC ATCCACCCCT 
ACGAGGAGTC GTGAGAACCA CGTCGAATTC AAGGACGAAA AGGAAGACAC CCTAAAACCC 
GCCCCCACCA TGGGTTCACC ACGAACGGGT CCTAGTTTGA CCCCCGGCAG CATCCTCCGC 
CAAGCCAACA GCACCAGCAA CATGCGGCGA CCACATCAGA GCATCCTCGG CGAGAACACT 
CCTCCTCAGT CGGCCACCAT GCTCGCCCTT CAGAACATGA GTAGTGCCAG CAACCTCGGA 
ACGTCGTCGT CGGTCAGGGA CTCGGATCCC CTCGCTAACA TCACTAACGG CTCCAGCGGA 
GTGACCAAGA CGACTGTAAC ACCCCTAGGC CTCGAGGCCC TGTCGAACCA GCTCATCACC 
CTTACCAACA TTGCTTCGGC TCTTCAACGA GAAATGTCGG CGCTTAGCAG GCGCAGCAGA 
GATAATGCTA CAGACTTGCT CAGCTTGAAA GAGGCCACCA ATCAGCGCGA TGAGGACATT 
CGCAGGAGTT TGCGAGAGTT GATCACCGAG GCCAAGTCGA GGGCCGCGCA GAGGGAAGCG 
TACGGTGGGG GTCCGCCATC GCCTACGTCG CTAAAGTCGC GACCGTTCTC CCTGCCGCGA 
ATACCTTCGC CACATAGCTT TGGACCGTCG CTTGATCGAG AGTCGCTATT AAGCACACCC 
TCACTTGTGG CCGACACATC GTCAATTGCT CTTCTAGAAA AGATCCTTCG CGACATGGGA 
ACGAAAGAGG GCCAAGAATC GCTTCTTTCG AGACTTAACG AACTCGCCCG TCAGCTTTCC 
GGCTTTTCCA CGTCTGAAAA GCTAGAGGAG ATCATCCGTT TGGTCAAAGC CAATCAGCAA 
CATGCTCTAG TGCCAGCTAC TGGCGGAGCA GGGAGAGGTG GTGGTGGCGG CGGCGGCGGC 
GGCGGTCGCA ACAAGGCCTG GAGCTTTAGC GAAGACGAAG ACGGCCGAAA CTCGCGCGTA 
GGCCGATTCC TCGAAGAATC TGGAAATTCA CCAACTCAAA CCCAAAGCTC CCGTGCCACC 
GATTCCATGA ATGATGAAAT CCTCTCCGCC CTGCGCATAG TCAAGGATCA CCTGGCCCAA 
GGCGGCAGCC TTACCAGCGA CGTCAAGTCT CTCGTCAAGG AACTGCGCGG CGAGGTCCTC 
GACATGGGCC AAGAAATCGG TCGCCGACTT AACGAAGTCT CCTCCAAGTC CCCTCAAACC 
CTCCCTGTAG ACAATTCTAC AAAGGAAGCC ATGAACAAAA TTGTGTCAGA TGGCCTGGAG 
GAAATGCGCC AGAACATGGT CAACATCGTG CACGAACACC GCAAGAAGGC GCAAGAGACA 
GTCGCGGCAG CGCCTCGAAT CGACTCCATG ATCGATTACA GGGAGATCTA CAATAGCATG 
CGTGCTGCCC TCAAGGATTC ACAAGCCAAT AAGCCGCGCG TGCAGGAGCT TCGTAGGGAG 
GACGTTGTAC AAGCAGTGCA AGATGCTTTC GAAAAGTACA AGCCTGAAAT TGAGATCCAG 
CAGATCGGAT TGGAAAGGCA CGAGGTGCTG GACTGTTTGA AGGAGGGGCT AGGAATGTAT 
CTGCCAAAGG AGACGGCTTC CAAGCAAGAC GTGTTTGCTG CCATCAACGA AGGGTTCAGC 
AAGTTTGAGA CGGTCTCCCG GGACGATGTC CTCGAATCTG TCAGGGAATG TCTGGACGAG 
TTCGACTTCC CTGTGACTCC CCAGCTTTCG AAAGACGACA TTATGAGTGC GGTCAAGGAT 
GGTTTGAGCG ACCTCCCCAA GCCCCTGAAG CGTCAAGAGG TGGTGAATGC CATGGATGAG 
AGCCTCAACA AGTTCGACTT CACTGTTGAC ATGGCCGAAG CCGTGCAAGA TGGTGTACGG 
TCTCTGGATC TGCCAAAGGA CATGCAAACC GCCGTCACCA AAGCGCTGGA GACATTCGAC 
TTTTCCTCTG CCGGCCCATC AAGCGAAGAC ATGATCGATG CTGTCCAGGA AGCTATCGAA 
ACCATGGATC TTCCCTGTGA TGTCACACGC GCCGTCGTCA AAGCATTGCA AACCCCCGAG 
TTCGCACAGC ATATCGCTTC TTACCTCCCC CGCCCCGATC TGTCGCGTGT CGACCTTGCT 
GATGCCGTCC GCGAAGGATT TGATGCGCTT GATATTTCAG GAGATGTCGC CCGCGCTGTC 
AAAGAAAGTC TAAAAGAACT AGACCTCTCC GATCTCTCCG GCTCACATTC CAAAGCTCTC 
GTCCGCCACG GCACCACGCC TCCTTCCATC CTGAAGCCCG AAACTGAAGA AATCATTGAG 
CGACTGTACG AAATCCGTGA TTCGCTAAGA GATGAGTTCC GCGCCTTCTC AGAAAAAAGC 
AAACCCACCT CCCGTGGTGG TGACGCTGAG CGGACTACAC CTGGTATTAC CATGGACGAT 
TTCGAGAAAC TGAGGCATGA CATCGAAGCG TACGTCGACC GCGCCCGCAC CGACGGCAAC 
TCCGACGATT TGATCCCGCA CATGACTGAA ACATTGGAGA GATTTAGGGA GGAAGTGGCG 
GAGTTGGTCA AGAAGGCTTC TCGGAGCACC AACGATCTGC TAAGGGAGGA ACTTGAATCA 
CTCCGCGACG TCGTCAACGG CACCTTGGTC CCCGTAACGC CACTCGCGCA GCCCGGCAGC 
AATGCCGAGA TTCTCGAGGC CATTGGTGCT ATTCGTAAGG AGATGAATAC CACCTCTCAG 
CACACCGGTG AGGCGGCGGA AACGAAAATC CTCCAAGCTC TGCAAGAGGG ACTCGGCGAT 
TTGAAAAAGA GTATTGACAA GCTCTCTAAC AAAGCCGGTG ACCTCTCGGC TAATGACGAG 
ATTCTGGATG CGCTCAAGGA GGGCCTGAAG GGCGTCAGAG CTGATCTCGA AGAGCTTAGA 
GCAGGAGGCG GGTTTGATGA CGAATTCGCG CTGACGCCCT TTAGCAAGGA TGGTGATCAA 
CCTTCGAAGC AGCTTGGCCA TGGAAGCGTC GAGGAGATTA GAAGTCTTCA TGCTATGGTT 
GATAAGCTAG GGAAGAAGGT CGAGGCTATG GAAAGGGGTG CTGGCCCGTC AAAGGCTGCG 
GCGGGTGCCA GAACTGCTAC ATCCAAGTCT GAGCTAGGCG ACGTCGAGGC CTTGCTAAGG 
AAGCTGATCG ATTCCGTCGC CTCTCTTGCT GCCCGCGAAC AAAAGCAGCA GAAGATCAAC 
CTGTCACCCC TCGAAGAAAC CATTCGCTCC CTCCAGACTT CCGTTGACAC CGCGATTGGG 
GGTCTGAGAA AGTCCATTGA TGAGTGGACC AAGGCAAGAT CGTCATCAGC CGCGCCTAAG 
CAGCCCGAGC CTGAGCTGCT TCCCACCAGC GACCCTACCG ATCCGGCTAC GAAAGAAGAT 
GTCCAGGCAA TTGAGACCAT TCTAAGGAAT ACCAAGGACA GCTTGGACGA TTTCGTGAGC 
GCCCAGGGAC TGCAGAGGGA GCTAGTCAGG AAAGAGCATG TGGATGCACT TGAAGTCTTG 
TTGGTAGAGA CAAAGTCGAA CTTGGGAGAT TTGTCGAAGC AAGTGGAAGG TGTGTCTAGG 
AAGGAGGATG TTTCCGCTCT CGAGTCGCTC ATCGCCAAGA TGACGATTGC CTTCGACGAA 
ATGAGGACCC GCGACGAGAA AGAAGACTCC AACAAGCTCA CCAAAGCTGA TCTCGAGGCT 
GTTGAGACTG TTTGTCTCGA AGTCAAGTCC CTCGTTTCAT CGAGACCCGC CACCACTGAT 
GCCACCACCG ACTCTCCGAC AGCCACAGCC AGCCGCCCCG ACATGGACAG CAACTTCAAG 
ACCCTGACTG AGAAGCTTGA AGCTCAAGCC GAGGCTACTA CAAAGGCAAA CGGCGACATC 
ACCGACCGCG TGTCCGAAGT CAAATCCCTC CTCGAAGAGT TTCATGAAGT CATCAAAGCT 
AAACTCACTG ATGGAGCCAA GGGGGTGGAT TCTATTCACA AGGTTCTGGC TCCAATGAGC 
GAAATTGTCA AGAAGAACGC GACGCTGCAG GATGAGCTCA TGGAATTGTG GGGTGGCATG 
AAGGAGGGTT TCGAGGAAGG TCGCACGACT ATCAATAAGG CGAAGACTGA CATTGAGACC 
AAGATCGAGG AGCTTCAGAA GGCGCAGATC TACGAGATCA AGGAGACCGT TGCGACAAAG 
GGCCAAATGG AGGAGCTCAA GACTGCGCTG GAGCCTCTCA GCGGTACGGT CACGGAGGCT 
GTGGAAAAGA TGGAAGTCAG CTCAAAGACT GTTTTCGAAA GGGTGGATGA GCTGGTGTGC 
AAGGCCGATG AGAATCATCT CGATATCAAG GCTGAGCATG CCCTTACCCG AGAGCAGGTG 
CAGGAGATGA CTGGCAAGTT GGACGGCTTG CAGAGTCTTG TTGCCGAGCA TCAGCCTAAG 
GTCGCCGAGA ATCTCCAGGA GGTTCTTGCC CTTGTTGGTC AGCATTTTGA GCATTCCAAG 
ACGACAACGG AGGGTCTGCA GACCAAGGTC ATCAACGCCA TGCCCACTGT CAACGAGAAG 
CTCGAAAAGC TCGTTGGTCA TACCGGCGAA GCGGAGAAGG CTTTCGCTCA TCTCGAAGAT 
GTCCACAAAC AAGTCAAGAA TACCTCCGCC GAGCTCGCTG AGTTCATCGC CGCCCAAACT 
CAACGCGCCG TCGAAGAGGA TGAGAACAAG AAGAAGATAC TGCAAGAGAC CACAATTGCT 
CTTGAGCGCC AGCTGGCTGA AAAGGAAGCA ATCGAAGCTC GTTTGGCTGA GCTCAAAGAG 
GAGGAAGCCC GCGTTAAGGA GAGCATTAAT GTTACTTTGC GTGAGGAGCA GGATCAGCTC 
AAGTCCCAGT TCTTGGAAAA CCTCCAACAT GAACAAGCAC GTCTGAAGCA GATGAATGAG 
TCTCTAAAGC AGGAACAGGA AGAGCTCAAG TCTACGTTCT TGGCCAACTT GGCTGAGGAG 
GAGCATCGCC TCAAGACGCT CAATGAGTCC CTAAGAAAAG AGCACGAGGA AGCCAAGGCG 
GTGTTCATGG CCGGTCTCAA GGAGGAGGAG ACGAGGCTGA AGGAACTCAA TGCTTCTCTC 
AAGGAAGAGC ACGAGACCTT GAAGAAGACG TTCACAATTA ATCTTGAAGA GGAACGCGAC 
CGCTTGATGG AAATCAACGT CGCCCTCAAA GAAGAACAAG ACACCATGAA GAAGACCTTT 
GCTGCTAACC TCCGCGATGA GCGCGACCGT CTGATCGAGC TCAATTCCGC CCTCAAAGCC 
GAGCACGAGT CGCTCAAGCG GAGCTTCCTT GCCGTTTTGA AGGACGAAGA AGCCCGTCTG 
AAGGAGTCGA ACGATGCTTT GCGAGAAGAG CAGCAAGCTC TCAAGGATAA GTTCCTCAAG 
AATTTGCGCG AGGAGGAGTC TTTGCTCAAG GAAGTCAACG AAGGTCTGAG GCAAGAGCAG 
AAAGAACTTA GGAGCTCTTT CCTTGCCGCT TTCAAGGAGG AAGAGAGGCG TCTCAAGGAG 
ATCAACGCCA GCCTGCGGGA AGAGCAAGAG GAAATGAAGG CGATCTTCCG TGAAGAGCAG 
GAGAAGCTGA AGGTGGATCT GCTCGCTAAC CTGATGGAGG AGGAGTGTCG CCTTAAAGAA 
CGCAACGAGG CACTGCGCAA GGAGCATGAC AAACTTAAGG AGCAGTTTTT GGCTGATCTG 
AGGGCTGAGC AGCAGGGTAT CAAGCACAAC CTTGTTGAGC TTCGCGGAGA GCAAGAGGAG 
CTGGTGCGCA AGAAATCCAG ACTCACGGCG GACTTGTCCT CGCTCGACAC TGCCCTCCGC 
CTCCGAAGAG AAGAGCTCCA CGAGATGGAA ACCCGCGCCG AGACACTCGA GCGCCGTATC 
CTCGAGGGAG TCATGGATCA TTCACGCTTA CTTCTCATGG CCAAAGGCAA GCGCTTCACC 
ACGGGCGTCG GTCGGGAGTC AATGAATCGC AAGCGCGTTC CCTCGCAAAT GTCCACCCGC 
GCTCCCAGCG TCGTTTCACG CATGGAGTCA AGACAGCCCA CGTCGGCACT CAACATGGCT 
GCTATCAACG CCAAGACCAG AACCCCTAAG ATGGGCACGG GTGCGGGATC GTCGGCGTCT 
AACAGACGGA TTCTGTCTCT GAGTCAAATG GTCAGCGGAG CTCTGCCGGG CGGCAGCATC 
AAGCGCAGCA ATAGCGTTAG GAACGGGGCT GGTGGTGGTG GTGTCGCGGC GGTCAAGGCG 
GCGGTAGCTG CTCATCATGG TGATGACGGT ACTTCCGGGA CTTCGACACC TAAGGCTAAA 
GAACGGAAGG GAAGCTGGGC GACGACGAGT TCCAAGTCCA CGGCGAAGGG GTATGGTGGT 
CTATCGAAAC AGCATAGAGA TTTCTCCCTG AAGGACACGG ATGATGAGGA CGAGGAGGGG 
TATGACACCG CTACTCCACC AGTTGATGTG CGCTCCGGGT CAGATGAGGA CATGGTTCAC 
GTCGACGACG ACGCGCGCAC TGAGTCCTCT TCCCGCTACG GCGGCGACGA AGACCACAAC 
GAAACCGAAA CCGAAACTGA GGCGGACGAA ACAGCCACTC TACGTCGCTC CTCCCATGGT 
ACGACGGTCA TCACTTCCTC TTCCCGCTCC GGCCCTCGTT CGGCTCGATC CGGCCGCTCA 
AATGGCAGTC GGGATGACGA CGGAGAAAGC CAGTACAGCC AGGACCACGA TCAAGATGAA 
GGAGACGACA ACTACTCGGA CTACGAAACA GGCGACGATA GTGAAGACGA CTGGACCGAG 
AGGGGTAGTG CCATTTCGGG CATGACTGGT ACCACGGGCA CGAGCAGGAC GGGGCGCAGT 
AGTGTTAGCG CGCCGTCGAT TGCGCCGAGT GCGTCGGTCA TGAGCGTGGC GAGTTCAAAG 
ATGTCAAAGG CTAGTAAGAT GAGTAAGGAT TCGGCTATTT CGGGGATTCC TAGGGCGAGT 
CCGAAGAAGG GGAAAAAGGC TGGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03578 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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