RAPGEF2 cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF2
Entrez Gene ID	110072791
Full Name	Rap guanine nucleotide exchange factor 2
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020781419.1	XP_020637078.1	rap guanine nucleotide exchange factor 2 isoform X2
XM_020781420.1	XP_020637079.1	rap guanine nucleotide exchange factor 2 isoform X3
XM_020781422.1	XP_020637081.1	rap guanine nucleotide exchange factor 2 isoform X5
XM_020781424.1	XP_020637083.1	rap guanine nucleotide exchange factor 2 isoform X6
XM_020781418.1	XP_020637077.1	rap guanine nucleotide exchange factor 2 isoform X1
XM_020781421.1	XP_020637080.1	rap guanine nucleotide exchange factor 2 isoform X4

Pathways from BioSystems
Gene Ontology

The following RAPGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPo234410	XM_020781424.1 Latest version!	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPo234409	XM_020781422.1 Latest version!	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo234405	XM_020781418.1 Latest version!	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPo234406	XM_020781419.1 Latest version!	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPo234407	XM_020781420.1 Latest version!	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPo234408	XM_020781421.1 Latest version!	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF2 ( XM_020781424.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020637083.1 Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPo234410
Clone ID Related Accession (Same CDS sequence)	XM_020781424.1
Accession Version	XM_020781424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	rap guanine nucleotide exchange factor 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150727.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGGAATG CACTAAACAG 
CAGCTTCCTG CAGATTTCAC AAAACTGCAC CTCACAGACA GTCTCCATCC ACAGGTGACC 
CACGTTTCAT CTAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG TAGCAGCCTA 
TCTGATATAT ACCAGGCAAC AGAAAGTGAA GCCGGTGACA TGGATCTAAG TGGTTTACCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ATATTGAGAG GGCATCTGAC 
CCTCTAATGA GCAGAGATAT TGTTAGAGAC TGCCTAGAAA AAGATCCAAT AGACAGGACA 
GATGATGATA TTGAACAACT CTTGGAATTC ATGCATCAGT TACCAGCTTT TGCTAATATG 
ACAATGTCTG TAAGGAGAGA ACTCTGTGCT GTAATGGTGT TTGCAGTTGT AGAAAGAGCA 
GGAACCATAG TTCTAAATGA TGGGGAAGAG CTGGACTCTT GGTCTGTTAT TTTGAATGGT 
TCTGTGGAAG TAACATATCC TGATGGAAGA ACAGAAATAT TATGCATGGG GAACAGTTTT 
GGTGTTTCTC CTACCATGGA CAAAGAGTAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GATTGCCAGT TTGTGTGCAT AGCTCAACAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAAAACATGC AGAAAGTAGA AGAGGAAGGA GAGATTGTTA TGGTAAAAGA GCATAGGGAA 
TTGGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTTATCA AGGGAACACC GGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCCGTA GTGGATCCAA CATTTATAGA GGATTTCCTT 
TTGACCTACA GGACCTTCCT TTCCAGTCCA ATGGAAGTGG GAAAGAAGTT ATTGGAGTGG 
TTCAATGATC CTAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAGGG AGATCCTGCA ATGACTCGTT TTTTAGAGGA ATTTGAGAAC 
AATTTGGAGA GAGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAACATAGC ATGTGCTGCT 
AAAGCTAAAC GGAGAATGAT AACCCTGACA AAGCCATCTC GGGAAGCCCC TTTGCCTTTC 
TTATTGTTGG GAGGATCTGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCCA CTGAGGCAGG CTTGAAACGT GGTGATCAGA TACTGGAAGT GAATGGCCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTCGAAATAA TACACATTTA 
TCTATCACTG TGAAAACCAA CCTGTTCGTT TTTAAAGAAC TTCTAGCAAG ATTATCCGAG 
GAGAAAAGAA ATGGTGCCCC TCATCTACCG AAAATTGGTG ATATTAAAAA AGCAAGCCGT 
TACTCCATTC CAGATCTTGC AGTTGATGTG GAACAGGTGA TAGGATTGGA AAAAGTAACC 
AAGAAAAGCA AAGCCAACAC TGTTGGTGGG CGAAACAAGT TAAAGAAGAT ACTAGACAAA 
ACCCGCATTA GTATCCTGCC TCAGAAACCA TACAATGATA TTGGAATTGG CCAGTCTCAG 
GATGACAGTA TTGTAGGACT GAGGCAGACC AAGCACATTC CTTCTGCTCT GCCAGTTAGT 
GGAACACTGT CCTCAAGCAA TCCTGACTTA TTGCAGTCAC ATCACCGCAT CTTAGATTTC 
AACACAACCC CAGATTTACC AGATCAGGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGC 
CGTTACATTA TGATCAGCAA GGACACAACT GCTAAGGAAG TGGTGATTCA GGCAATACGA 
GAATTTGCTC TAACTACCAC CCCAGATGCA TATTCTCTGT GTGAAGTCTC AGTCACTCCA 
GAGGGTGTCA TTAAACAACG GAGGCTCCCA GATCAACTCT CCAAGCTTGC TGATAGAATA 
CAACTTAGTG GAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020637083.1
CDS	567..3104
Translation	MASYVDNNFRQAVMKNPADRSPQDLEIVYSYLHGMEALSNLREHQLRMMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDAYPVGKPPLPRGYHTECTKQQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTPERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRMITLTKPSREAPLPFLLLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLARLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVTKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPSALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGR

Target ORF information:

RefSeq Version	XM_020781424.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020781424.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGGAATG CACTAAACAG 
CAGCTTCCTG CAGATTTCAC AAAACTGCAC CTCACAGACA GTCTCCATCC ACAGGTGACC 
CACGTTTCAT CTAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG TAGCAGCCTA 
TCTGATATAT ACCAGGCAAC AGAAAGTGAA GCCGGTGACA TGGATCTAAG TGGTTTACCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ATATTGAGAG GGCATCTGAC 
CCTCTAATGA GCAGAGATAT TGTTAGAGAC TGCCTAGAAA AAGATCCAAT AGACAGGACA 
GATGATGATA TTGAACAACT CTTGGAATTC ATGCATCAGT TACCAGCTTT TGCTAATATG 
ACAATGTCTG TAAGGAGAGA ACTCTGTGCT GTAATGGTGT TTGCAGTTGT AGAAAGAGCA 
GGAACCATAG TTCTAAATGA TGGGGAAGAG CTGGACTCTT GGTCTGTTAT TTTGAATGGT 
TCTGTGGAAG TAACATATCC TGATGGAAGA ACAGAAATAT TATGCATGGG GAACAGTTTT 
GGTGTTTCTC CTACCATGGA CAAAGAGTAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GATTGCCAGT TTGTGTGCAT AGCTCAACAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAAAACATGC AGAAAGTAGA AGAGGAAGGA GAGATTGTTA TGGTAAAAGA GCATAGGGAA 
TTGGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTTATCA AGGGAACACC GGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCCGTA GTGGATCCAA CATTTATAGA GGATTTCCTT 
TTGACCTACA GGACCTTCCT TTCCAGTCCA ATGGAAGTGG GAAAGAAGTT ATTGGAGTGG 
TTCAATGATC CTAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAGGG AGATCCTGCA ATGACTCGTT TTTTAGAGGA ATTTGAGAAC 
AATTTGGAGA GAGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAACATAGC ATGTGCTGCT 
AAAGCTAAAC GGAGAATGAT AACCCTGACA AAGCCATCTC GGGAAGCCCC TTTGCCTTTC 
TTATTGTTGG GAGGATCTGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCCA CTGAGGCAGG CTTGAAACGT GGTGATCAGA TACTGGAAGT GAATGGCCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTCGAAATAA TACACATTTA 
TCTATCACTG TGAAAACCAA CCTGTTCGTT TTTAAAGAAC TTCTAGCAAG ATTATCCGAG 
GAGAAAAGAA ATGGTGCCCC TCATCTACCG AAAATTGGTG ATATTAAAAA AGCAAGCCGT 
TACTCCATTC CAGATCTTGC AGTTGATGTG GAACAGGTGA TAGGATTGGA AAAAGTAACC 
AAGAAAAGCA AAGCCAACAC TGTTGGTGGG CGAAACAAGT TAAAGAAGAT ACTAGACAAA 
ACCCGCATTA GTATCCTGCC TCAGAAACCA TACAATGATA TTGGAATTGG CCAGTCTCAG 
GATGACAGTA TTGTAGGACT GAGGCAGACC AAGCACATTC CTTCTGCTCT GCCAGTTAGT 
GGAACACTGT CCTCAAGCAA TCCTGACTTA TTGCAGTCAC ATCACCGCAT CTTAGATTTC 
AACACAACCC CAGATTTACC AGATCAGGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGC 
CGTTACATTA TGATCAGCAA GGACACAACT GCTAAGGAAG TGGTGATTCA GGCAATACGA 
GAATTTGCTC TAACTACCAC CCCAGATGCA TATTCTCTGT GTGAAGTCTC AGTCACTCCA 
GAGGGTGTCA TTAAACAACG GAGGCTCCCA GATCAACTCT CCAAGCTTGC TGATAGAATA 
CAACTTAGTG GAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_020781422.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020637081.1 Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPo234409
Clone ID Related Accession (Same CDS sequence)	XM_020781422.1
Accession Version	XM_020781422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	rap guanine nucleotide exchange factor 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150727.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCACCTC ACAGACAGTC TCCATCCACA GGTGACCCAC 
GTTTCATCTA GTCATTCAGG ATGTAGCATC ACCAGTGACT CTGGAAGTAG CAGCCTATCT 
GATATATACC AGGCAACAGA AAGTGAAGCC GGTGACATGG ATCTAAGTGG TTTACCAGAG 
ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGATA TTGAGAGGGC ATCTGACCCT 
CTAATGAGCA GAGATATTGT TAGAGACTGC CTAGAAAAAG ATCCAATAGA CAGGACAGAT 
GATGATATTG AACAACTCTT GGAATTCATG CATCAGTTAC CAGCTTTTGC TAATATGACA 
ATGTCTGTAA GGAGAGAACT CTGTGCTGTA ATGGTGTTTG CAGTTGTAGA AAGAGCAGGA 
ACCATAGTTC TAAATGATGG GGAAGAGCTG GACTCTTGGT CTGTTATTTT GAATGGTTCT 
GTGGAAGTAA CATATCCTGA TGGAAGAACA GAAATATTAT GCATGGGGAA CAGTTTTGGT 
GTTTCTCCTA CCATGGACAA AGAGTATATG AAAGGAGTGA TGAGAACCAA AGTGGATGAT 
TGCCAGTTTG TGTGCATAGC TCAACAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA 
AACATGCAGA AAGTAGAAGA GGAAGGAGAG ATTGTTATGG TAAAAGAGCA TAGGGAATTG 
GATCGCACTG GAACAAGAAA AGGTCACATT GTTATCAAGG GAACACCGGA AAGGTTAACA 
ATGCATTTGG TGGAAGAACA TTCCGTAGTG GATCCAACAT TTATAGAGGA TTTCCTTTTG 
ACCTACAGGA CCTTCCTTTC CAGTCCAATG GAAGTGGGAA AGAAGTTATT GGAGTGGTTC 
AATGATCCTA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC 
TTCAATGATT TTGAGGGAGA TCCTGCAATG ACTCGTTTTT TAGAGGAATT TGAGAACAAT 
TTGGAGAGAG AGAAAATGGG TGGACATTTG AGGCTGTTAA ACATAGCATG TGCTGCTAAA 
GCTAAACGGA GAATGATAAC CCTGACAAAG CCATCTCGGG AAGCCCCTTT GCCTTTCTTA 
TTGTTGGGAG GATCTGAAAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCCACTG AGGCAGGCTT GAAACGTGGT GATCAGATAC TGGAAGTGAA TGGCCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTC GAAATAATAC ACATTTATCT 
ATCACTGTGA AAACCAACCT GTTCGTTTTT AAAGAACTTC TAGCAAGATT ATCCGAGGAG 
AAAAGAAATG GTGCCCCTCA TCTACCGAAA ATTGGTGATA TTAAAAAAGC AAGCCGTTAC 
TCCATTCCAG ATCTTGCAGT TGATGTGGAA CAGGTGATAG GATTGGAAAA AGTAACCAAG 
AAAAGCAAAG CCAACACTGT TGGTGGGCGA AACAAGTTAA AGAAGATACT AGACAAAACC 
CGCATTAGTA TCCTGCCTCA GAAACCATAC AATGATATTG GAATTGGCCA GTCTCAGGAT 
GACAGTATTG TAGGACTGAG GCAGACCAAG CACATTCCTT CTGCTCTGCC AGTTAGTGGA 
ACACTGTCCT CAAGCAATCC TGACTTATTG CAGTCACATC ACCGCATCTT AGATTTCAAC 
ACAACCCCAG ATTTACCAGA TCAGGTTCTG AGGGTTTTCA AGGCAGACCA GCAAAGCCGT 
TACATTATGA TCAGCAAGGA CACAACTGCT AAGGAAGTGG TGATTCAGGC AATACGAGAA 
TTTGCTCTAA CTACCACCCC AGATGCATAT TCTCTGTGTG AAGTCTCAGT CACTCCAGAG 
GGTGTCATTA AACAACGGAG GCTCCCAGAT CAACTCTCCA AGCTTGCTGA TAGAATACAA 
CTTAGTGGAA GGTATTATCT GAAGAATAAT ATGGAAACAG AAACATTGTG TTCGGATGAA 
GATGCTCAGG AATTGCTGAG AGAGAGCCAA ATTTCTCTAC TGCAACTCAG TACTGTTGAG 
GTGGCTACTC AGCTCTCAAT GCGTAACTTT GAATTGTTCC GGAACATTGA ACCTACAGAG 
TATATAGATG ACTTGTTTAA ACTGAAATCT AAATCTAGTT GTGCTAACTT GAAAAAGTTT 
GAAGAAGTAA TAAACCAGGA AACCTTTTGG GTAGCATCTG AGATTCTGCG AGAAACCAAC 
CAGCTGAAAA GGATGAAGAT AATTAAGCAC TTCATTAAGA TAGCATTGCA CTGCAGGGAA 
TGCAAGAACT TCAACTCCAT GTTTGCAATA ATAAGTGGCC TGAATTTAGC TCCAGTTGCA 
AGACTACGTA CCACATGGGA AAAACTTCCA AGCAAATATG AAAAATTGTT TCAAGATCTT 
CAGGATTTGT TTGATCCATC TAGAAACATG GCAAAATACC GCAATGTCTT GAACAGCCAA 
AATCTACAGC CCCCAATTAT CCCACTATTC CCTGTAATCA AAAAGGACCT TACATTTCTT 
CATGAAGGAA ATGACTCCAA AGTAGAAGGT TTAGTCAACT TCGAGAAACT ACGAATGATT 
GCAAAAGAGA TTCGCCATGT GGGTCGAATG GCTTCTGTTA ACATGGATCC TGCTTTAATG 
TTCAGGACTA GGAAGAAGAA GTGGAGGAGT CTGGGATCCC TCAGTCAAGG CAGTACAAAT 
GCAGCAGTAC TAGATGTTGC ACAAACAGGT GGTCATAAAA AGCGGGTCCG TCGCAGTTCC 
TTCCTCAATG CCAAAAAGCT GTATGAAGAT GCCCAGATGG CTCGTAAAGT CAAGCAGTAT 
CTCTCTAATT TGGACCTAGA GATGGATGAA GAAAGCCTCC AGGCACTCTC CCTGCAGTGT 
GAACCAGCTA GCAATACATT ACCTAAGACC CCAGGGGAAA AGCGGTCTGG GAAACCTGAA 
ACATCACCAG TTGCACACAG GTCAGGGATC CAGCAAAAGG TGCAGCAGCA ACAGCAGCAC 
AAAGCAAACC AAGCATTGCA AGTCCCTGCT GTGTCACTTT ACCCCTCTCG TAAGAAAGTG 
CCGGTCAAGG ACCTCCCGCC TTTTGGCATA AACTCTCCAC AAGCTTTAAA GAAAATACTT 
TCGTTGTCTG AAGAGGGAAG TATAGACCGT CATAAAAAGC AAGCTGAGGA CACTATTTCA 
AATGCCTCAT CACAGCTATC TTCTCCCCCC ACCTCCCCTC AGAGCTCTCC CCGGAAAGGT 
TTTACCTTAG CTGCCAGTGG AACAGTGGAT AACTTTTCAG ACTCTGGTCA CAGTGAAATT 
TCCTCCAGAT CTAGCATTGT CAGCAATTCT TCATTTGACT CTATGCCAGT ATCGCTACAT 
GATGAAAGGA GACAGAGGCA CTCTGTCAGC ATTGTGGAGA CTAATCTTGG AGTGGGCAGA 
ATTGATAGAA GAGCAATTAT TGAACCAGAT CAGTACAGCC TAAGCTCCTA CGCACCACTG 
TCAGAGAACA GAGGTTTGTA TGCTGGAGCA ACCATGCTTT CTTCTCCCAG TACGGAAGAG 
TTATCCCAGG ATCAGGGAGA TCGAGCATCA CTTGATGCAG CAGATAGTGG TCGTGGCAGT 
TGGACTTCTT GTTCAAGTGG TTCCCATGAC AACATACAGA CAATACAGCA CCAGCGAAGT 
TGGGAGACTC TTCCTTTTGG ACATGCTCAT TTCGATAGTT CAGGCGATGG GGCAGGACTG 
TGGGCCTCAG GCAGCCACAT GGACCCGATG ATGTTCTCCG AACATGGCAC AAAGTATGGC 
AGGCAGAGTC AAGGTAGGGA GGGCCTTGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC 
TCCACAGGCT ACTGGGGAGA GGACTCTGAA GGTGATACAG GTACCATAAA ACGGAGGGGT 
GGGAAGGATG TGTCAATTGA AGCTGAAACC AGTAGCATAA CATCAATAAC AGCAGAGGAT 
TCAAAGCCAG TTCCCATGCC AGCTCATATA GCTGTATCAA CAAGTAATGC AAAGGGTCTT 
ATTGCTCGAA AAGAGGGCCG CTATCGTGAA CCACCACCAA CCCCACCTGG TTACGTGGGA 
ATACCTATTG ACTTTGCAGA AGGAGTTTCC CACCCAGTCC GAAAACCTCC AGATTACAAC 
GTAGCACTTC AGAGGTCTAG GATGGTGGCG CGGTCAAATG AAGCCCCTGG GACTTCAGCT 
TCACATCAGC AAACACAGGG TCATTCATCT GGCAGGCCTG TTAATAAACC TCAGTGGCAC 
AAACCAAACG AGTCAGACCC ACATCTTGCT CACTATCAGT CTCAAGTGTT TTCCACAGAG 
GAGGATGAAG ATGAACAAGT TTCTGCCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020637081.1
CDS	444..4916
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTPERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRMITLTKPSREAPLPFLLLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLARLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVTKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPSALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKSSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQALSLQCEPASNTLPKTPGEKRSGKPETSPVAHRSGIQQKVQQQQQHKANQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSIDRHKKQAEDTISNASSQLSSPPTSPQSSPRKGFTLAASGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRAIIEPDQYSLSSYAPLSENRGLYAGATMLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDPMMFSEHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSITAEDSKPVPMPAHIAVSTSNAKGLIARKEGRYREPPPTPPGYVGIPIDFAEGVSHPVRKPPDYNVALQRSRMVARSNEAPGTSASHQQTQGHSSGRPVNKPQWHKPNESDPHLAHYQSQVFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_020781422.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020781422.1

ORF Insert Sequence:

ATGAAACCTC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCACCTC ACAGACAGTC TCCATCCACA GGTGACCCAC 
GTTTCATCTA GTCATTCAGG ATGTAGCATC ACCAGTGACT CTGGAAGTAG CAGCCTATCT 
GATATATACC AGGCAACAGA AAGTGAAGCC GGTGACATGG ATCTAAGTGG TTTACCAGAG 
ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGATA TTGAGAGGGC ATCTGACCCT 
CTAATGAGCA GAGATATTGT TAGAGACTGC CTAGAAAAAG ATCCAATAGA CAGGACAGAT 
GATGATATTG AACAACTCTT GGAATTCATG CATCAGTTAC CAGCTTTTGC TAATATGACA 
ATGTCTGTAA GGAGAGAACT CTGTGCTGTA ATGGTGTTTG CAGTTGTAGA AAGAGCAGGA 
ACCATAGTTC TAAATGATGG GGAAGAGCTG GACTCTTGGT CTGTTATTTT GAATGGTTCT 
GTGGAAGTAA CATATCCTGA TGGAAGAACA GAAATATTAT GCATGGGGAA CAGTTTTGGT 
GTTTCTCCTA CCATGGACAA AGAGTATATG AAAGGAGTGA TGAGAACCAA AGTGGATGAT 
TGCCAGTTTG TGTGCATAGC TCAACAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAA 
AACATGCAGA AAGTAGAAGA GGAAGGAGAG ATTGTTATGG TAAAAGAGCA TAGGGAATTG 
GATCGCACTG GAACAAGAAA AGGTCACATT GTTATCAAGG GAACACCGGA AAGGTTAACA 
ATGCATTTGG TGGAAGAACA TTCCGTAGTG GATCCAACAT TTATAGAGGA TTTCCTTTTG 
ACCTACAGGA CCTTCCTTTC CAGTCCAATG GAAGTGGGAA AGAAGTTATT GGAGTGGTTC 
AATGATCCTA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC 
TTCAATGATT TTGAGGGAGA TCCTGCAATG ACTCGTTTTT TAGAGGAATT TGAGAACAAT 
TTGGAGAGAG AGAAAATGGG TGGACATTTG AGGCTGTTAA ACATAGCATG TGCTGCTAAA 
GCTAAACGGA GAATGATAAC CCTGACAAAG CCATCTCGGG AAGCCCCTTT GCCTTTCTTA 
TTGTTGGGAG GATCTGAAAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCCACTG AGGCAGGCTT GAAACGTGGT GATCAGATAC TGGAAGTGAA TGGCCAAAAC 
TTTGAAAATA TTCAGCTGTC AAAAGCCATG GAAATTCTTC GAAATAATAC ACATTTATCT 
ATCACTGTGA AAACCAACCT GTTCGTTTTT AAAGAACTTC TAGCAAGATT ATCCGAGGAG 
AAAAGAAATG GTGCCCCTCA TCTACCGAAA ATTGGTGATA TTAAAAAAGC AAGCCGTTAC 
TCCATTCCAG ATCTTGCAGT TGATGTGGAA CAGGTGATAG GATTGGAAAA AGTAACCAAG 
AAAAGCAAAG CCAACACTGT TGGTGGGCGA AACAAGTTAA AGAAGATACT AGACAAAACC 
CGCATTAGTA TCCTGCCTCA GAAACCATAC AATGATATTG GAATTGGCCA GTCTCAGGAT 
GACAGTATTG TAGGACTGAG GCAGACCAAG CACATTCCTT CTGCTCTGCC AGTTAGTGGA 
ACACTGTCCT CAAGCAATCC TGACTTATTG CAGTCACATC ACCGCATCTT AGATTTCAAC 
ACAACCCCAG ATTTACCAGA TCAGGTTCTG AGGGTTTTCA AGGCAGACCA GCAAAGCCGT 
TACATTATGA TCAGCAAGGA CACAACTGCT AAGGAAGTGG TGATTCAGGC AATACGAGAA 
TTTGCTCTAA CTACCACCCC AGATGCATAT TCTCTGTGTG AAGTCTCAGT CACTCCAGAG 
GGTGTCATTA AACAACGGAG GCTCCCAGAT CAACTCTCCA AGCTTGCTGA TAGAATACAA 
CTTAGTGGAA GGTATTATCT GAAGAATAAT ATGGAAACAG AAACATTGTG TTCGGATGAA 
GATGCTCAGG AATTGCTGAG AGAGAGCCAA ATTTCTCTAC TGCAACTCAG TACTGTTGAG 
GTGGCTACTC AGCTCTCAAT GCGTAACTTT GAATTGTTCC GGAACATTGA ACCTACAGAG 
TATATAGATG ACTTGTTTAA ACTGAAATCT AAATCTAGTT GTGCTAACTT GAAAAAGTTT 
GAAGAAGTAA TAAACCAGGA AACCTTTTGG GTAGCATCTG AGATTCTGCG AGAAACCAAC 
CAGCTGAAAA GGATGAAGAT AATTAAGCAC TTCATTAAGA TAGCATTGCA CTGCAGGGAA 
TGCAAGAACT TCAACTCCAT GTTTGCAATA ATAAGTGGCC TGAATTTAGC TCCAGTTGCA 
AGACTACGTA CCACATGGGA AAAACTTCCA AGCAAATATG AAAAATTGTT TCAAGATCTT 
CAGGATTTGT TTGATCCATC TAGAAACATG GCAAAATACC GCAATGTCTT GAACAGCCAA 
AATCTACAGC CCCCAATTAT CCCACTATTC CCTGTAATCA AAAAGGACCT TACATTTCTT 
CATGAAGGAA ATGACTCCAA AGTAGAAGGT TTAGTCAACT TCGAGAAACT ACGAATGATT 
GCAAAAGAGA TTCGCCATGT GGGTCGAATG GCTTCTGTTA ACATGGATCC TGCTTTAATG 
TTCAGGACTA GGAAGAAGAA GTGGAGGAGT CTGGGATCCC TCAGTCAAGG CAGTACAAAT 
GCAGCAGTAC TAGATGTTGC ACAAACAGGT GGTCATAAAA AGCGGGTCCG TCGCAGTTCC 
TTCCTCAATG CCAAAAAGCT GTATGAAGAT GCCCAGATGG CTCGTAAAGT CAAGCAGTAT 
CTCTCTAATT TGGACCTAGA GATGGATGAA GAAAGCCTCC AGGCACTCTC CCTGCAGTGT 
GAACCAGCTA GCAATACATT ACCTAAGACC CCAGGGGAAA AGCGGTCTGG GAAACCTGAA 
ACATCACCAG TTGCACACAG GTCAGGGATC CAGCAAAAGG TGCAGCAGCA ACAGCAGCAC 
AAAGCAAACC AAGCATTGCA AGTCCCTGCT GTGTCACTTT ACCCCTCTCG TAAGAAAGTG 
CCGGTCAAGG ACCTCCCGCC TTTTGGCATA AACTCTCCAC AAGCTTTAAA GAAAATACTT 
TCGTTGTCTG AAGAGGGAAG TATAGACCGT CATAAAAAGC AAGCTGAGGA CACTATTTCA 
AATGCCTCAT CACAGCTATC TTCTCCCCCC ACCTCCCCTC AGAGCTCTCC CCGGAAAGGT 
TTTACCTTAG CTGCCAGTGG AACAGTGGAT AACTTTTCAG ACTCTGGTCA CAGTGAAATT 
TCCTCCAGAT CTAGCATTGT CAGCAATTCT TCATTTGACT CTATGCCAGT ATCGCTACAT 
GATGAAAGGA GACAGAGGCA CTCTGTCAGC ATTGTGGAGA CTAATCTTGG AGTGGGCAGA 
ATTGATAGAA GAGCAATTAT TGAACCAGAT CAGTACAGCC TAAGCTCCTA CGCACCACTG 
TCAGAGAACA GAGGTTTGTA TGCTGGAGCA ACCATGCTTT CTTCTCCCAG TACGGAAGAG 
TTATCCCAGG ATCAGGGAGA TCGAGCATCA CTTGATGCAG CAGATAGTGG TCGTGGCAGT 
TGGACTTCTT GTTCAAGTGG TTCCCATGAC AACATACAGA CAATACAGCA CCAGCGAAGT 
TGGGAGACTC TTCCTTTTGG ACATGCTCAT TTCGATAGTT CAGGCGATGG GGCAGGACTG 
TGGGCCTCAG GCAGCCACAT GGACCCGATG ATGTTCTCCG AACATGGCAC AAAGTATGGC 
AGGCAGAGTC AAGGTAGGGA GGGCCTTGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC 
TCCACAGGCT ACTGGGGAGA GGACTCTGAA GGTGATACAG GTACCATAAA ACGGAGGGGT 
GGGAAGGATG TGTCAATTGA AGCTGAAACC AGTAGCATAA CATCAATAAC AGCAGAGGAT 
TCAAAGCCAG TTCCCATGCC AGCTCATATA GCTGTATCAA CAAGTAATGC AAAGGGTCTT 
ATTGCTCGAA AAGAGGGCCG CTATCGTGAA CCACCACCAA CCCCACCTGG TTACGTGGGA 
ATACCTATTG ACTTTGCAGA AGGAGTTTCC CACCCAGTCC GAAAACCTCC AGATTACAAC 
GTAGCACTTC AGAGGTCTAG GATGGTGGCG CGGTCAAATG AAGCCCCTGG GACTTCAGCT 
TCACATCAGC AAACACAGGG TCATTCATCT GGCAGGCCTG TTAATAAACC TCAGTGGCAC 
AAACCAAACG AGTCAGACCC ACATCTTGCT CACTATCAGT CTCAAGTGTT TTCCACAGAG 
GAGGATGAAG ATGAACAAGT TTCTGCCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_020781418.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020637077.1 Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

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CloneID	OPo234405
Clone ID Related Accession (Same CDS sequence)	XM_020781418.1
Accession Version	XM_020781418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	rap guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150727.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGGAATG CACTAAACAG 
CAGCTTCCTG CAGATTTCAC AAAACTGCAC CTCACAGACA GTCTCCATCC ACAGGTGACC 
CACGTTTCAT CTAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG TAGCAGCCTA 
TCTGATATAT ACCAGGCAAC AGAAAGTGAA GCCGGTGACA TGGATCTAAG TGGTTTACCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ATATTGAGAG GGCATCTGAC 
CCTCTAATGA GCAGAGATAT TGTTAGAGAC TGCCTAGAAA AAGATCCAAT AGACAGGACA 
GATGATGATA TTGAACAACT CTTGGAATTC ATGCATCAGT TACCAGCTTT TGCTAATATG 
ACAATGTCTG TAAGGAGAGA ACTCTGTGCT GTAATGGTGT TTGCAGTTGT AGAAAGAGCA 
GGAACCATAG TTCTAAATGA TGGGGAAGAG CTGGACTCTT GGTCTGTTAT TTTGAATGGT 
TCTGTGGAAG TAACATATCC TGATGGAAGA ACAGAAATAT TATGCATGGG GAACAGTTTT 
GGTGTTTCTC CTACCATGGA CAAAGAGTAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GATTGCCAGT TTGTGTGCAT AGCTCAACAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAAAACATGC AGAAAGTAGA AGAGGAAGGA GAGATTGTTA TGGTAAAAGA GCATAGGGAA 
TTGGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTTATCA AGGGAACACC GGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCCGTA GTGGATCCAA CATTTATAGA GGATTTCCTT 
TTGACCTACA GGACCTTCCT TTCCAGTCCA ATGGAAGTGG GAAAGAAGTT ATTGGAGTGG 
TTCAATGATC CTAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAGGG AGATCCTGCA ATGACTCGTT TTTTAGAGGA ATTTGAGAAC 
AATTTGGAGA GAGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAACATAGC ATGTGCTGCT 
AAAGCTAAAC GGAGAATGAT AACCCTGACA AAGCCATCTC GGGAAGCCCC TTTGCCTTTC 
TTATTGTTGG GAGGATCTGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCCA CTGAGGCAGG CTTGAAACGT GGTGATCAGA TACTGGAAGT GAATGGCCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTCGAAATAA TACACATTTA 
TCTATCACTG TGAAAACCAA CCTGTTCGTT TTTAAAGAAC TTCTAGCAAG ATTATCCGAG 
GAGAAAAGAA ATGGTGCCCC TCATCTACCG AAAATTGGTG ATATTAAAAA AGCAAGCCGT 
TACTCCATTC CAGATCTTGC AGTTGATGTG GAACAGGTGA TAGGATTGGA AAAAGTAACC 
AAGAAAAGCA AAGCCAACAC TGTTGGTGGG CGAAACAAGT TAAAGAAGAT ACTAGACAAA 
ACCCGCATTA GTATCCTGCC TCAGAAACCA TACAATGATA TTGGAATTGG CCAGTCTCAG 
GATGACAGTA TTGTAGGACT GAGGCAGACC AAGCACATTC CTTCTGCTCT GCCAGTTAGT 
GGAACACTGT CCTCAAGCAA TCCTGACTTA TTGCAGTCAC ATCACCGCAT CTTAGATTTC 
AACACAACCC CAGATTTACC AGATCAGGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGC 
CGTTACATTA TGATCAGCAA GGACACAACT GCTAAGGAAG TGGTGATTCA GGCAATACGA 
GAATTTGCTC TAACTACCAC CCCAGATGCA TATTCTCTGT GTGAAGTCTC AGTCACTCCA 
GAGGGTGTCA TTAAACAACG GAGGCTCCCA GATCAACTCT CCAAGCTTGC TGATAGAATA 
CAACTTAGTG GAAGGTATTA TCTGAAGAAT AATATGGAAA CAGAAACATT GTGTTCGGAT 
GAAGATGCTC AGGAATTGCT GAGAGAGAGC CAAATTTCTC TACTGCAACT CAGTACTGTT 
GAGGTGGCTA CTCAGCTCTC AATGCGTAAC TTTGAATTGT TCCGGAACAT TGAACCTACA 
GAGTATATAG ATGACTTGTT TAAACTGAAA TCTAAATCTA GTTGTGCTAA CTTGAAAAAG 
TTTGAAGAAG TAATAAACCA GGAAACCTTT TGGGTAGCAT CTGAGATTCT GCGAGAAACC 
AACCAGCTGA AAAGGATGAA GATAATTAAG CACTTCATTA AGATAGCATT GCACTGCAGG 
GAATGCAAGA ACTTCAACTC CATGTTTGCA ATAATAAGTG GCCTGAATTT AGCTCCAGTT 
GCAAGACTAC GTACCACATG GGAAAAACTT CCAAGCAAAT ATGAAAAATT GTTTCAAGAT 
CTTCAGGATT TGTTTGATCC ATCTAGAAAC ATGGCAAAAT ACCGCAATGT CTTGAACAGC 
CAAAATCTAC AGCCCCCAAT TATCCCACTA TTCCCTGTAA TCAAAAAGGA CCTTACATTT 
CTTCATGAAG GAAATGACTC CAAAGTAGAA GGTTTAGTCA ACTTCGAGAA ACTACGAATG 
ATTGCAAAAG AGATTCGCCA TGTGGGTCGA ATGGCTTCTG TTAACATGGA TCCTGCTTTA 
ATGTTCAGGA CTAGGAAGAA GAAGTGGAGG AGTCTGGGAT CCCTCAGTCA AGGCAGTACA 
AATGCAGCAG TACTAGATGT TGCACAAACA GGTGGTCATA AAAAGCGGGT CCGTCGCAGT 
TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGTAA AGTCAAGCAG 
TATCTCTCTA ATTTGGACCT AGAGATGGAT GAAGAAAGCC TCCAGGCACT CTCCCTGCAG 
TGTGAACCAG CTAGCAATAC ATTACCTAAG ACCCCAGGGG AAAAGCGGTC TGGGAAACCT 
GAAACATCAC CAGTTGCACA CAGGTCAGGG ATCCAGCAAA AGGTGCAGCA GCAACAGCAG 
CACAAAGCAA ACCAAGCATT GCAAGTCCCT GCTGTGTCAC TTTACCCCTC TCGTAAGAAA 
GTGCCGGTCA AGGACCTCCC GCCTTTTGGC ATAAACTCTC CACAAGCTTT AAAGAAAATA 
CTTTCGTTGT CTGAAGAGGG AAGTATAGAC CGTCATAAAA AGCAAGCTGA GGACACTATT 
TCAAATGCCT CATCACAGCT ATCTTCTCCC CCCACCTCCC CTCAGAGCTC TCCCCGGAAA 
GGTTTTACCT TAGCTGCCAG TGGAACAGTG GATAACTTTT CAGACTCTGG TCACAGTGAA 
ATTTCCTCCA GATCTAGCAT TGTCAGCAAT TCTTCATTTG ACTCTATGCC AGTATCGCTA 
CATGATGAAA GGAGACAGAG GCACTCTGTC AGCATTGTGG AGACTAATCT TGGAGTGGGC 
AGAATTGATA GAAGAGCAAT TATTGAACCA GATCAGTACA GCCTAAGCTC CTACGCACCA 
CTGTCAGAGA ACAGAGGTTT GTATGCTGGA GCAACCATGC TTTCTTCTCC CAGTACGGAA 
GAGTTATCCC AGGATCAGGG AGATCGAGCA TCACTTGATG CAGCAGATAG TGGTCGTGGC 
AGTTGGACTT CTTGTTCAAG TGGTTCCCAT GACAACATAC AGACAATACA GCACCAGCGA 
AGTTGGGAGA CTCTTCCTTT TGGACATGCT CATTTCGATA GTTCAGGCGA TGGGGCAGGA 
CTGTGGGCCT CAGGCAGCCA CATGGACCCG ATGATGTTCT CCGAACATGG CACAAAGTAT 
GGCAGGCAGA GTCAAGGTAG GGAGGGCCTT GATCAAGCAC AGTCCAGAGC AAGCTGGGCA 
TCCTCCACAG GCTACTGGGG AGAGGACTCT GAAGGTGATA CAGGTACCAT AAAACGGAGG 
GGTGGGAAGG ATGTGTCAAT TGAAGCTGAA ACCAGTAGCA TAACATCAAT AACAGCAGAG 
GATTCAAAGC CAGTTCCCAT GCCAGCTCAT ATAGCTGTAT CAACAAGTAA TGCAAAGGGT 
CTTATTGCTC GAAAAGAGGG CCGCTATCGT GAACCACCAC CAACCCCACC TGGTTACGTG 
GGAATACCTA TTGACTTTGC AGAAGGAGTT TCCCACCCAG TCCGAAAACC TCCAGATTAC 
AACGTAGCAC TTCAGAGGTC TAGGATGGTG GCGCGGTCAA ATGAAGCCCC TGGGACTTCA 
GCTTCACATC AGCAAACACA GGGTCATTCA TCTGGCAGGC CTGTTAATAA ACCTCAGTGG 
CACAAACCAA ACGAGTCAGA CCCACATCTT GCTCACTATC AGTCTCAAGT GTTTTCCACA 
GAGGAGGATG AAGATGAACA AGTTTCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020637077.1
CDS	567..5522
Translation	MASYVDNNFRQAVMKNPADRSPQDLEIVYSYLHGMEALSNLREHQLRMMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDAYPVGKPPLPRGYHTECTKQQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTPERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRMITLTKPSREAPLPFLLLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLARLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVTKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPSALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKSSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQALSLQCEPASNTLPKTPGEKRSGKPETSPVAHRSGIQQKVQQQQQHKANQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSIDRHKKQAEDTISNASSQLSSPPTSPQSSPRKGFTLAASGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRAIIEPDQYSLSSYAPLSENRGLYAGATMLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDPMMFSEHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSITAEDSKPVPMPAHIAVSTSNAKGLIARKEGRYREPPPTPPGYVGIPIDFAEGVSHPVRKPPDYNVALQRSRMVARSNEAPGTSASHQQTQGHSSGRPVNKPQWHKPNESDPHLAHYQSQVFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_020781418.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020781418.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGGAATG CACTAAACAG 
CAGCTTCCTG CAGATTTCAC AAAACTGCAC CTCACAGACA GTCTCCATCC ACAGGTGACC 
CACGTTTCAT CTAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG TAGCAGCCTA 
TCTGATATAT ACCAGGCAAC AGAAAGTGAA GCCGGTGACA TGGATCTAAG TGGTTTACCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ATATTGAGAG GGCATCTGAC 
CCTCTAATGA GCAGAGATAT TGTTAGAGAC TGCCTAGAAA AAGATCCAAT AGACAGGACA 
GATGATGATA TTGAACAACT CTTGGAATTC ATGCATCAGT TACCAGCTTT TGCTAATATG 
ACAATGTCTG TAAGGAGAGA ACTCTGTGCT GTAATGGTGT TTGCAGTTGT AGAAAGAGCA 
GGAACCATAG TTCTAAATGA TGGGGAAGAG CTGGACTCTT GGTCTGTTAT TTTGAATGGT 
TCTGTGGAAG TAACATATCC TGATGGAAGA ACAGAAATAT TATGCATGGG GAACAGTTTT 
GGTGTTTCTC CTACCATGGA CAAAGAGTAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GATTGCCAGT TTGTGTGCAT AGCTCAACAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAAAACATGC AGAAAGTAGA AGAGGAAGGA GAGATTGTTA TGGTAAAAGA GCATAGGGAA 
TTGGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTTATCA AGGGAACACC GGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCCGTA GTGGATCCAA CATTTATAGA GGATTTCCTT 
TTGACCTACA GGACCTTCCT TTCCAGTCCA ATGGAAGTGG GAAAGAAGTT ATTGGAGTGG 
TTCAATGATC CTAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAGGG AGATCCTGCA ATGACTCGTT TTTTAGAGGA ATTTGAGAAC 
AATTTGGAGA GAGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAACATAGC ATGTGCTGCT 
AAAGCTAAAC GGAGAATGAT AACCCTGACA AAGCCATCTC GGGAAGCCCC TTTGCCTTTC 
TTATTGTTGG GAGGATCTGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCCA CTGAGGCAGG CTTGAAACGT GGTGATCAGA TACTGGAAGT GAATGGCCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTCGAAATAA TACACATTTA 
TCTATCACTG TGAAAACCAA CCTGTTCGTT TTTAAAGAAC TTCTAGCAAG ATTATCCGAG 
GAGAAAAGAA ATGGTGCCCC TCATCTACCG AAAATTGGTG ATATTAAAAA AGCAAGCCGT 
TACTCCATTC CAGATCTTGC AGTTGATGTG GAACAGGTGA TAGGATTGGA AAAAGTAACC 
AAGAAAAGCA AAGCCAACAC TGTTGGTGGG CGAAACAAGT TAAAGAAGAT ACTAGACAAA 
ACCCGCATTA GTATCCTGCC TCAGAAACCA TACAATGATA TTGGAATTGG CCAGTCTCAG 
GATGACAGTA TTGTAGGACT GAGGCAGACC AAGCACATTC CTTCTGCTCT GCCAGTTAGT 
GGAACACTGT CCTCAAGCAA TCCTGACTTA TTGCAGTCAC ATCACCGCAT CTTAGATTTC 
AACACAACCC CAGATTTACC AGATCAGGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGC 
CGTTACATTA TGATCAGCAA GGACACAACT GCTAAGGAAG TGGTGATTCA GGCAATACGA 
GAATTTGCTC TAACTACCAC CCCAGATGCA TATTCTCTGT GTGAAGTCTC AGTCACTCCA 
GAGGGTGTCA TTAAACAACG GAGGCTCCCA GATCAACTCT CCAAGCTTGC TGATAGAATA 
CAACTTAGTG GAAGGTATTA TCTGAAGAAT AATATGGAAA CAGAAACATT GTGTTCGGAT 
GAAGATGCTC AGGAATTGCT GAGAGAGAGC CAAATTTCTC TACTGCAACT CAGTACTGTT 
GAGGTGGCTA CTCAGCTCTC AATGCGTAAC TTTGAATTGT TCCGGAACAT TGAACCTACA 
GAGTATATAG ATGACTTGTT TAAACTGAAA TCTAAATCTA GTTGTGCTAA CTTGAAAAAG 
TTTGAAGAAG TAATAAACCA GGAAACCTTT TGGGTAGCAT CTGAGATTCT GCGAGAAACC 
AACCAGCTGA AAAGGATGAA GATAATTAAG CACTTCATTA AGATAGCATT GCACTGCAGG 
GAATGCAAGA ACTTCAACTC CATGTTTGCA ATAATAAGTG GCCTGAATTT AGCTCCAGTT 
GCAAGACTAC GTACCACATG GGAAAAACTT CCAAGCAAAT ATGAAAAATT GTTTCAAGAT 
CTTCAGGATT TGTTTGATCC ATCTAGAAAC ATGGCAAAAT ACCGCAATGT CTTGAACAGC 
CAAAATCTAC AGCCCCCAAT TATCCCACTA TTCCCTGTAA TCAAAAAGGA CCTTACATTT 
CTTCATGAAG GAAATGACTC CAAAGTAGAA GGTTTAGTCA ACTTCGAGAA ACTACGAATG 
ATTGCAAAAG AGATTCGCCA TGTGGGTCGA ATGGCTTCTG TTAACATGGA TCCTGCTTTA 
ATGTTCAGGA CTAGGAAGAA GAAGTGGAGG AGTCTGGGAT CCCTCAGTCA AGGCAGTACA 
AATGCAGCAG TACTAGATGT TGCACAAACA GGTGGTCATA AAAAGCGGGT CCGTCGCAGT 
TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGTAA AGTCAAGCAG 
TATCTCTCTA ATTTGGACCT AGAGATGGAT GAAGAAAGCC TCCAGGCACT CTCCCTGCAG 
TGTGAACCAG CTAGCAATAC ATTACCTAAG ACCCCAGGGG AAAAGCGGTC TGGGAAACCT 
GAAACATCAC CAGTTGCACA CAGGTCAGGG ATCCAGCAAA AGGTGCAGCA GCAACAGCAG 
CACAAAGCAA ACCAAGCATT GCAAGTCCCT GCTGTGTCAC TTTACCCCTC TCGTAAGAAA 
GTGCCGGTCA AGGACCTCCC GCCTTTTGGC ATAAACTCTC CACAAGCTTT AAAGAAAATA 
CTTTCGTTGT CTGAAGAGGG AAGTATAGAC CGTCATAAAA AGCAAGCTGA GGACACTATT 
TCAAATGCCT CATCACAGCT ATCTTCTCCC CCCACCTCCC CTCAGAGCTC TCCCCGGAAA 
GGTTTTACCT TAGCTGCCAG TGGAACAGTG GATAACTTTT CAGACTCTGG TCACAGTGAA 
ATTTCCTCCA GATCTAGCAT TGTCAGCAAT TCTTCATTTG ACTCTATGCC AGTATCGCTA 
CATGATGAAA GGAGACAGAG GCACTCTGTC AGCATTGTGG AGACTAATCT TGGAGTGGGC 
AGAATTGATA GAAGAGCAAT TATTGAACCA GATCAGTACA GCCTAAGCTC CTACGCACCA 
CTGTCAGAGA ACAGAGGTTT GTATGCTGGA GCAACCATGC TTTCTTCTCC CAGTACGGAA 
GAGTTATCCC AGGATCAGGG AGATCGAGCA TCACTTGATG CAGCAGATAG TGGTCGTGGC 
AGTTGGACTT CTTGTTCAAG TGGTTCCCAT GACAACATAC AGACAATACA GCACCAGCGA 
AGTTGGGAGA CTCTTCCTTT TGGACATGCT CATTTCGATA GTTCAGGCGA TGGGGCAGGA 
CTGTGGGCCT CAGGCAGCCA CATGGACCCG ATGATGTTCT CCGAACATGG CACAAAGTAT 
GGCAGGCAGA GTCAAGGTAG GGAGGGCCTT GATCAAGCAC AGTCCAGAGC AAGCTGGGCA 
TCCTCCACAG GCTACTGGGG AGAGGACTCT GAAGGTGATA CAGGTACCAT AAAACGGAGG 
GGTGGGAAGG ATGTGTCAAT TGAAGCTGAA ACCAGTAGCA TAACATCAAT AACAGCAGAG 
GATTCAAAGC CAGTTCCCAT GCCAGCTCAT ATAGCTGTAT CAACAAGTAA TGCAAAGGGT 
CTTATTGCTC GAAAAGAGGG CCGCTATCGT GAACCACCAC CAACCCCACC TGGTTACGTG 
GGAATACCTA TTGACTTTGC AGAAGGAGTT TCCCACCCAG TCCGAAAACC TCCAGATTAC 
AACGTAGCAC TTCAGAGGTC TAGGATGGTG GCGCGGTCAA ATGAAGCCCC TGGGACTTCA 
GCTTCACATC AGCAAACACA GGGTCATTCA TCTGGCAGGC CTGTTAATAA ACCTCAGTGG 
CACAAACCAA ACGAGTCAGA CCCACATCTT GCTCACTATC AGTCTCAAGT GTTTTCCACA 
GAGGAGGATG AAGATGAACA AGTTTCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_020781419.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020637078.1 Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

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CloneID	OPo234406
Clone ID Related Accession (Same CDS sequence)	XM_020781419.1
Accession Version	XM_020781419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	rap guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150727.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGCTTCC TGCAGATTTC 
ACAAAACTGC ACCTCACAGA CAGTCTCCAT CCACAGGTGA CCCACGTTTC ATCTAGTCAT 
TCAGGATGTA GCATCACCAG TGACTCTGGA AGTAGCAGCC TATCTGATAT ATACCAGGCA 
ACAGAAAGTG AAGCCGGTGA CATGGATCTA AGTGGTTTAC CAGAGACAGC AGTGGATTCT 
GAGGATGATG ATGATGAAGA AGATATTGAG AGGGCATCTG ACCCTCTAAT GAGCAGAGAT 
ATTGTTAGAG ACTGCCTAGA AAAAGATCCA ATAGACAGGA CAGATGATGA TATTGAACAA 
CTCTTGGAAT TCATGCATCA GTTACCAGCT TTTGCTAATA TGACAATGTC TGTAAGGAGA 
GAACTCTGTG CTGTAATGGT GTTTGCAGTT GTAGAAAGAG CAGGAACCAT AGTTCTAAAT 
GATGGGGAAG AGCTGGACTC TTGGTCTGTT ATTTTGAATG GTTCTGTGGA AGTAACATAT 
CCTGATGGAA GAACAGAAAT ATTATGCATG GGGAACAGTT TTGGTGTTTC TCCTACCATG 
GACAAAGAGT ATATGAAAGG AGTGATGAGA ACCAAAGTGG ATGATTGCCA GTTTGTGTGC 
ATAGCTCAAC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAAGTA 
GAAGAGGAAG GAGAGATTGT TATGGTAAAA GAGCATAGGG AATTGGATCG CACTGGAACA 
AGAAAAGGTC ACATTGTTAT CAAGGGAACA CCGGAAAGGT TAACAATGCA TTTGGTGGAA 
GAACATTCCG TAGTGGATCC AACATTTATA GAGGATTTCC TTTTGACCTA CAGGACCTTC 
CTTTCCAGTC CAATGGAAGT GGGAAAGAAG TTATTGGAGT GGTTCAATGA TCCTAGCCTC 
AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAG 
GGAGATCCTG CAATGACTCG TTTTTTAGAG GAATTTGAGA ACAATTTGGA GAGAGAGAAA 
ATGGGTGGAC ATTTGAGGCT GTTAAACATA GCATGTGCTG CTAAAGCTAA ACGGAGAATG 
ATAACCCTGA CAAAGCCATC TCGGGAAGCC CCTTTGCCTT TCTTATTGTT GGGAGGATCT 
GAAAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC CACTGAGGCA 
GGCTTGAAAC GTGGTGATCA GATACTGGAA GTGAATGGCC AAAACTTTGA AAATATTCAG 
CTGTCAAAAG CCATGGAAAT TCTTCGAAAT AATACACATT TATCTATCAC TGTGAAAACC 
AACCTGTTCG TTTTTAAAGA ACTTCTAGCA AGATTATCCG AGGAGAAAAG AAATGGTGCC 
CCTCATCTAC CGAAAATTGG TGATATTAAA AAAGCAAGCC GTTACTCCAT TCCAGATCTT 
GCAGTTGATG TGGAACAGGT GATAGGATTG GAAAAAGTAA CCAAGAAAAG CAAAGCCAAC 
ACTGTTGGTG GGCGAAACAA GTTAAAGAAG ATACTAGACA AAACCCGCAT TAGTATCCTG 
CCTCAGAAAC CATACAATGA TATTGGAATT GGCCAGTCTC AGGATGACAG TATTGTAGGA 
CTGAGGCAGA CCAAGCACAT TCCTTCTGCT CTGCCAGTTA GTGGAACACT GTCCTCAAGC 
AATCCTGACT TATTGCAGTC ACATCACCGC ATCTTAGATT TCAACACAAC CCCAGATTTA 
CCAGATCAGG TTCTGAGGGT TTTCAAGGCA GACCAGCAAA GCCGTTACAT TATGATCAGC 
AAGGACACAA CTGCTAAGGA AGTGGTGATT CAGGCAATAC GAGAATTTGC TCTAACTACC 
ACCCCAGATG CATATTCTCT GTGTGAAGTC TCAGTCACTC CAGAGGGTGT CATTAAACAA 
CGGAGGCTCC CAGATCAACT CTCCAAGCTT GCTGATAGAA TACAACTTAG TGGAAGGTAT 
TATCTGAAGA ATAATATGGA AACAGAAACA TTGTGTTCGG ATGAAGATGC TCAGGAATTG 
CTGAGAGAGA GCCAAATTTC TCTACTGCAA CTCAGTACTG TTGAGGTGGC TACTCAGCTC 
TCAATGCGTA ACTTTGAATT GTTCCGGAAC ATTGAACCTA CAGAGTATAT AGATGACTTG 
TTTAAACTGA AATCTAAATC TAGTTGTGCT AACTTGAAAA AGTTTGAAGA AGTAATAAAC 
CAGGAAACCT TTTGGGTAGC ATCTGAGATT CTGCGAGAAA CCAACCAGCT GAAAAGGATG 
AAGATAATTA AGCACTTCAT TAAGATAGCA TTGCACTGCA GGGAATGCAA GAACTTCAAC 
TCCATGTTTG CAATAATAAG TGGCCTGAAT TTAGCTCCAG TTGCAAGACT ACGTACCACA 
TGGGAAAAAC TTCCAAGCAA ATATGAAAAA TTGTTTCAAG ATCTTCAGGA TTTGTTTGAT 
CCATCTAGAA ACATGGCAAA ATACCGCAAT GTCTTGAACA GCCAAAATCT ACAGCCCCCA 
ATTATCCCAC TATTCCCTGT AATCAAAAAG GACCTTACAT TTCTTCATGA AGGAAATGAC 
TCCAAAGTAG AAGGTTTAGT CAACTTCGAG AAACTACGAA TGATTGCAAA AGAGATTCGC 
CATGTGGGTC GAATGGCTTC TGTTAACATG GATCCTGCTT TAATGTTCAG GACTAGGAAG 
AAGAAGTGGA GGAGTCTGGG ATCCCTCAGT CAAGGCAGTA CAAATGCAGC AGTACTAGAT 
GTTGCACAAA CAGGTGGTCA TAAAAAGCGG GTCCGTCGCA GTTCCTTCCT CAATGCCAAA 
AAGCTGTATG AAGATGCCCA GATGGCTCGT AAAGTCAAGC AGTATCTCTC TAATTTGGAC 
CTAGAGATGG ATGAAGAAAG CCTCCAGGCA CTCTCCCTGC AGTGTGAACC AGCTAGCAAT 
ACATTACCTA AGACCCCAGG GGAAAAGCGG TCTGGGAAAC CTGAAACATC ACCAGTTGCA 
CACAGGTCAG GGATCCAGCA AAAGGTGCAG CAGCAACAGC AGCACAAAGC AAACCAAGCA 
TTGCAAGTCC CTGCTGTGTC ACTTTACCCC TCTCGTAAGA AAGTGCCGGT CAAGGACCTC 
CCGCCTTTTG GCATAAACTC TCCACAAGCT TTAAAGAAAA TACTTTCGTT GTCTGAAGAG 
GGAAGTATAG ACCGTCATAA AAAGCAAGCT GAGGACACTA TTTCAAATGC CTCATCACAG 
CTATCTTCTC CCCCCACCTC CCCTCAGAGC TCTCCCCGGA AAGGTTTTAC CTTAGCTGCC 
AGTGGAACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCCTC CAGATCTAGC 
ATTGTCAGCA ATTCTTCATT TGACTCTATG CCAGTATCGC TACATGATGA AAGGAGACAG 
AGGCACTCTG TCAGCATTGT GGAGACTAAT CTTGGAGTGG GCAGAATTGA TAGAAGAGCA 
ATTATTGAAC CAGATCAGTA CAGCCTAAGC TCCTACGCAC CACTGTCAGA GAACAGAGGT 
TTGTATGCTG GAGCAACCAT GCTTTCTTCT CCCAGTACGG AAGAGTTATC CCAGGATCAG 
GGAGATCGAG CATCACTTGA TGCAGCAGAT AGTGGTCGTG GCAGTTGGAC TTCTTGTTCA 
AGTGGTTCCC ATGACAACAT ACAGACAATA CAGCACCAGC GAAGTTGGGA GACTCTTCCT 
TTTGGACATG CTCATTTCGA TAGTTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGC 
CACATGGACC CGATGATGTT CTCCGAACAT GGCACAAAGT ATGGCAGGCA GAGTCAAGGT 
AGGGAGGGCC TTGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCCAC AGGCTACTGG 
GGAGAGGACT CTGAAGGTGA TACAGGTACC ATAAAACGGA GGGGTGGGAA GGATGTGTCA 
ATTGAAGCTG AAACCAGTAG CATAACATCA ATAACAGCAG AGGATTCAAA GCCAGTTCCC 
ATGCCAGCTC ATATAGCTGT ATCAACAAGT AATGCAAAGG GTCTTATTGC TCGAAAAGAG 
GGCCGCTATC GTGAACCACC ACCAACCCCA CCTGGTTACG TGGGAATACC TATTGACTTT 
GCAGAAGGAG TTTCCCACCC AGTCCGAAAA CCTCCAGATT ACAACGTAGC ACTTCAGAGG 
TCTAGGATGG TGGCGCGGTC AAATGAAGCC CCTGGGACTT CAGCTTCACA TCAGCAAACA 
CAGGGTCATT CATCTGGCAG GCCTGTTAAT AAACCTCAGT GGCACAAACC AAACGAGTCA 
GACCCACATC TTGCTCACTA TCAGTCTCAA GTGTTTTCCA CAGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020637078.1
CDS	567..5504
Translation	MASYVDNNFRQAVMKNPADRSPQDLEIVYSYLHGMEALSNLREHQLRMMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDAYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTPERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRMITLTKPSREAPLPFLLLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLARLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVTKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPSALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKSSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQALSLQCEPASNTLPKTPGEKRSGKPETSPVAHRSGIQQKVQQQQQHKANQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSIDRHKKQAEDTISNASSQLSSPPTSPQSSPRKGFTLAASGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRAIIEPDQYSLSSYAPLSENRGLYAGATMLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDPMMFSEHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSITAEDSKPVPMPAHIAVSTSNAKGLIARKEGRYREPPPTPPGYVGIPIDFAEGVSHPVRKPPDYNVALQRSRMVARSNEAPGTSASHQQTQGHSSGRPVNKPQWHKPNESDPHLAHYQSQVFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_020781419.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020781419.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGCTTCC TGCAGATTTC 
ACAAAACTGC ACCTCACAGA CAGTCTCCAT CCACAGGTGA CCCACGTTTC ATCTAGTCAT 
TCAGGATGTA GCATCACCAG TGACTCTGGA AGTAGCAGCC TATCTGATAT ATACCAGGCA 
ACAGAAAGTG AAGCCGGTGA CATGGATCTA AGTGGTTTAC CAGAGACAGC AGTGGATTCT 
GAGGATGATG ATGATGAAGA AGATATTGAG AGGGCATCTG ACCCTCTAAT GAGCAGAGAT 
ATTGTTAGAG ACTGCCTAGA AAAAGATCCA ATAGACAGGA CAGATGATGA TATTGAACAA 
CTCTTGGAAT TCATGCATCA GTTACCAGCT TTTGCTAATA TGACAATGTC TGTAAGGAGA 
GAACTCTGTG CTGTAATGGT GTTTGCAGTT GTAGAAAGAG CAGGAACCAT AGTTCTAAAT 
GATGGGGAAG AGCTGGACTC TTGGTCTGTT ATTTTGAATG GTTCTGTGGA AGTAACATAT 
CCTGATGGAA GAACAGAAAT ATTATGCATG GGGAACAGTT TTGGTGTTTC TCCTACCATG 
GACAAAGAGT ATATGAAAGG AGTGATGAGA ACCAAAGTGG ATGATTGCCA GTTTGTGTGC 
ATAGCTCAAC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAAAACAT GCAGAAAGTA 
GAAGAGGAAG GAGAGATTGT TATGGTAAAA GAGCATAGGG AATTGGATCG CACTGGAACA 
AGAAAAGGTC ACATTGTTAT CAAGGGAACA CCGGAAAGGT TAACAATGCA TTTGGTGGAA 
GAACATTCCG TAGTGGATCC AACATTTATA GAGGATTTCC TTTTGACCTA CAGGACCTTC 
CTTTCCAGTC CAATGGAAGT GGGAAAGAAG TTATTGGAGT GGTTCAATGA TCCTAGCCTC 
AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAG 
GGAGATCCTG CAATGACTCG TTTTTTAGAG GAATTTGAGA ACAATTTGGA GAGAGAGAAA 
ATGGGTGGAC ATTTGAGGCT GTTAAACATA GCATGTGCTG CTAAAGCTAA ACGGAGAATG 
ATAACCCTGA CAAAGCCATC TCGGGAAGCC CCTTTGCCTT TCTTATTGTT GGGAGGATCT 
GAAAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC CACTGAGGCA 
GGCTTGAAAC GTGGTGATCA GATACTGGAA GTGAATGGCC AAAACTTTGA AAATATTCAG 
CTGTCAAAAG CCATGGAAAT TCTTCGAAAT AATACACATT TATCTATCAC TGTGAAAACC 
AACCTGTTCG TTTTTAAAGA ACTTCTAGCA AGATTATCCG AGGAGAAAAG AAATGGTGCC 
CCTCATCTAC CGAAAATTGG TGATATTAAA AAAGCAAGCC GTTACTCCAT TCCAGATCTT 
GCAGTTGATG TGGAACAGGT GATAGGATTG GAAAAAGTAA CCAAGAAAAG CAAAGCCAAC 
ACTGTTGGTG GGCGAAACAA GTTAAAGAAG ATACTAGACA AAACCCGCAT TAGTATCCTG 
CCTCAGAAAC CATACAATGA TATTGGAATT GGCCAGTCTC AGGATGACAG TATTGTAGGA 
CTGAGGCAGA CCAAGCACAT TCCTTCTGCT CTGCCAGTTA GTGGAACACT GTCCTCAAGC 
AATCCTGACT TATTGCAGTC ACATCACCGC ATCTTAGATT TCAACACAAC CCCAGATTTA 
CCAGATCAGG TTCTGAGGGT TTTCAAGGCA GACCAGCAAA GCCGTTACAT TATGATCAGC 
AAGGACACAA CTGCTAAGGA AGTGGTGATT CAGGCAATAC GAGAATTTGC TCTAACTACC 
ACCCCAGATG CATATTCTCT GTGTGAAGTC TCAGTCACTC CAGAGGGTGT CATTAAACAA 
CGGAGGCTCC CAGATCAACT CTCCAAGCTT GCTGATAGAA TACAACTTAG TGGAAGGTAT 
TATCTGAAGA ATAATATGGA AACAGAAACA TTGTGTTCGG ATGAAGATGC TCAGGAATTG 
CTGAGAGAGA GCCAAATTTC TCTACTGCAA CTCAGTACTG TTGAGGTGGC TACTCAGCTC 
TCAATGCGTA ACTTTGAATT GTTCCGGAAC ATTGAACCTA CAGAGTATAT AGATGACTTG 
TTTAAACTGA AATCTAAATC TAGTTGTGCT AACTTGAAAA AGTTTGAAGA AGTAATAAAC 
CAGGAAACCT TTTGGGTAGC ATCTGAGATT CTGCGAGAAA CCAACCAGCT GAAAAGGATG 
AAGATAATTA AGCACTTCAT TAAGATAGCA TTGCACTGCA GGGAATGCAA GAACTTCAAC 
TCCATGTTTG CAATAATAAG TGGCCTGAAT TTAGCTCCAG TTGCAAGACT ACGTACCACA 
TGGGAAAAAC TTCCAAGCAA ATATGAAAAA TTGTTTCAAG ATCTTCAGGA TTTGTTTGAT 
CCATCTAGAA ACATGGCAAA ATACCGCAAT GTCTTGAACA GCCAAAATCT ACAGCCCCCA 
ATTATCCCAC TATTCCCTGT AATCAAAAAG GACCTTACAT TTCTTCATGA AGGAAATGAC 
TCCAAAGTAG AAGGTTTAGT CAACTTCGAG AAACTACGAA TGATTGCAAA AGAGATTCGC 
CATGTGGGTC GAATGGCTTC TGTTAACATG GATCCTGCTT TAATGTTCAG GACTAGGAAG 
AAGAAGTGGA GGAGTCTGGG ATCCCTCAGT CAAGGCAGTA CAAATGCAGC AGTACTAGAT 
GTTGCACAAA CAGGTGGTCA TAAAAAGCGG GTCCGTCGCA GTTCCTTCCT CAATGCCAAA 
AAGCTGTATG AAGATGCCCA GATGGCTCGT AAAGTCAAGC AGTATCTCTC TAATTTGGAC 
CTAGAGATGG ATGAAGAAAG CCTCCAGGCA CTCTCCCTGC AGTGTGAACC AGCTAGCAAT 
ACATTACCTA AGACCCCAGG GGAAAAGCGG TCTGGGAAAC CTGAAACATC ACCAGTTGCA 
CACAGGTCAG GGATCCAGCA AAAGGTGCAG CAGCAACAGC AGCACAAAGC AAACCAAGCA 
TTGCAAGTCC CTGCTGTGTC ACTTTACCCC TCTCGTAAGA AAGTGCCGGT CAAGGACCTC 
CCGCCTTTTG GCATAAACTC TCCACAAGCT TTAAAGAAAA TACTTTCGTT GTCTGAAGAG 
GGAAGTATAG ACCGTCATAA AAAGCAAGCT GAGGACACTA TTTCAAATGC CTCATCACAG 
CTATCTTCTC CCCCCACCTC CCCTCAGAGC TCTCCCCGGA AAGGTTTTAC CTTAGCTGCC 
AGTGGAACAG TGGATAACTT TTCAGACTCT GGTCACAGTG AAATTTCCTC CAGATCTAGC 
ATTGTCAGCA ATTCTTCATT TGACTCTATG CCAGTATCGC TACATGATGA AAGGAGACAG 
AGGCACTCTG TCAGCATTGT GGAGACTAAT CTTGGAGTGG GCAGAATTGA TAGAAGAGCA 
ATTATTGAAC CAGATCAGTA CAGCCTAAGC TCCTACGCAC CACTGTCAGA GAACAGAGGT 
TTGTATGCTG GAGCAACCAT GCTTTCTTCT CCCAGTACGG AAGAGTTATC CCAGGATCAG 
GGAGATCGAG CATCACTTGA TGCAGCAGAT AGTGGTCGTG GCAGTTGGAC TTCTTGTTCA 
AGTGGTTCCC ATGACAACAT ACAGACAATA CAGCACCAGC GAAGTTGGGA GACTCTTCCT 
TTTGGACATG CTCATTTCGA TAGTTCAGGC GATGGGGCAG GACTGTGGGC CTCAGGCAGC 
CACATGGACC CGATGATGTT CTCCGAACAT GGCACAAAGT ATGGCAGGCA GAGTCAAGGT 
AGGGAGGGCC TTGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCCAC AGGCTACTGG 
GGAGAGGACT CTGAAGGTGA TACAGGTACC ATAAAACGGA GGGGTGGGAA GGATGTGTCA 
ATTGAAGCTG AAACCAGTAG CATAACATCA ATAACAGCAG AGGATTCAAA GCCAGTTCCC 
ATGCCAGCTC ATATAGCTGT ATCAACAAGT AATGCAAAGG GTCTTATTGC TCGAAAAGAG 
GGCCGCTATC GTGAACCACC ACCAACCCCA CCTGGTTACG TGGGAATACC TATTGACTTT 
GCAGAAGGAG TTTCCCACCC AGTCCGAAAA CCTCCAGATT ACAACGTAGC ACTTCAGAGG 
TCTAGGATGG TGGCGCGGTC AAATGAAGCC CCTGGGACTT CAGCTTCACA TCAGCAAACA 
CAGGGTCATT CATCTGGCAG GCCTGTTAAT AAACCTCAGT GGCACAAACC AAACGAGTCA 
GACCCACATC TTGCTCACTA TCAGTCTCAA GTGTTTTCCA CAGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_020781420.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020637079.1 Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPo234407
Clone ID Related Accession (Same CDS sequence)	XM_020781420.1
Accession Version	XM_020781420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150727.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGGAATG CACTAAACAG 
CAGCTTCCTG CAGATTTCAC AAAACTGCAC CTCACAGACA GTCTCCATCC ACAGGTGACC 
CACGTTTCAT CTAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG TAGCAGCCTA 
TCTGATATAT ACCAGGCAAC AGAAAGTGAA GCCGGTGACA TGGATCTAAG TGGTTTACCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ATATTGAGAG GGCATCTGAC 
CCTCTAATGA GCAGAGATAT TGTTAGAGAC TGCCTAGAAA AAGATCCAAT AGACAGGACA 
GATGATGATA TTGAACAACT CTTGGAATTC ATGCATCAGT TACCAGCTTT TGCTAATATG 
ACAATGTCTG TAAGGAGAGA ACTCTGTGCT GTAATGGTGT TTGCAGTTGT AGAAAGAGCA 
GGAACCATAG TTCTAAATGA TGGGGAAGAG CTGGACTCTT GGTCTGTTAT TTTGAATGGT 
TCTGTGGAAG TAACATATCC TGATGGAAGA ACAGAAATAT TATGCATGGG GAACAGTTTT 
GGTGTTTCTC CTACCATGGA CAAAGAGTAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GATTGCCAGT TTGTGTGCAT AGCTCAACAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAAAACATGC AGAAAGTAGA AGAGGAAGGA GAGATTGTTA TGGTAAAAGA GCATAGGGAA 
TTGGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTTATCA AGGGAACACC GGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCCGTA GTGGATCCAA CATTTATAGA GGATTTCCTT 
TTGACCTACA GGACCTTCCT TTCCAGTCCA ATGGAAGTGG GAAAGAAGTT ATTGGAGTGG 
TTCAATGATC CTAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAGGG AGATCCTGCA ATGACTCGTT TTTTAGAGGA ATTTGAGAAC 
AATTTGGAGA GAGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAACATAGC ATGTGCTGCT 
AAAGCTAAAC GGAGAATGAT AACCCTGACA AAGCCATCTC GGGAAGCCCC TTTGCCTTTC 
TTATTGTTGG GAGGATCTGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCCA CTGAGGCAGG CTTGAAACGT GGTGATCAGA TACTGGAAGT GAATGGCCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTCGAAATAA TACACATTTA 
TCTATCACTG TGAAAACCAA CCTGTTCGTT TTTAAAGAAC TTCTAGCAAG ATTATCCGAG 
GAGAAAAGAA ATGGTGCCCC TCATCTACCG AAAATTGGTG ATATTAAAAA AGCAAGCCGT 
TACTCCATTC CAGATCTTGC AGTTGATGTG GAACAGGTGA TAGGATTGGA AAAAGTAACC 
AAGAAAAGCA AAGCCAACAC TGTTGGTGGG CGAAACAAGT TAAAGAAGAT ACTAGACAAA 
ACCCGCATTA GTATCCTGCC TCAGAAACCA TACAATGATA TTGGAATTGG CCAGTCTCAG 
GATGACAGTA TTGTAGGACT GAGGCAGACC AAGCACATTC CTTCTGCTCT GCCAGTTAGT 
GGAACACTGT CCTCAAGCAA TCCTGACTTA TTGCAGTCAC ATCACCGCAT CTTAGATTTC 
AACACAACCC CAGATTTACC AGATCAGGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGC 
CGTTACATTA TGATCAGCAA GGACACAACT GCTAAGGAAG TGGTGATTCA GGCAATACGA 
GAATTTGCTC TAACTACCAC CCCAGATGCA TATTCTCTGT GTGAAGTCTC AGTCACTCCA 
GAGGGTGTCA TTAAACAACG GAGGCTCCCA GATCAACTCT CCAAGCTTGC TGATAGAATA 
CAACTTAGTG GAAGGTATTA TCTGAAGAAT AATATGGAAA CAGAAACATT GTGTTCGGAT 
GAAGATGCTC AGGAATTGCT GAGAGAGAGC CAAATTTCTC TACTGCAACT CAGTACTGTT 
GAGGTGGCTA CTCAGCTCTC AATGCGTAAC TTTGAATTGT TCCGGAACAT TGAACCTACA 
GAGTATATAG ATGACTTGTT TAAACTGAAA TCTAAATCTA GTTGTGCTAA CTTGAAAAAG 
TTTGAAGAAG TAATAAACCA GGAAACCTTT TGGGTAGCAT CTGAGATTCT GCGAGAAACC 
AACCAGCTGA AAAGGATGAA GATAATTAAG CACTTCATTA AGATAGCATT GCACTGCAGG 
GAATGCAAGA ACTTCAACTC CATGTTTGCA ATAATAAGTG GCCTGAATTT AGCTCCAGTT 
GCAAGACTAC GTACCACATG GGAAAAACTT CCAAGCAAAT ATGAAAAATT GTTTCAAGAT 
CTTCAGGATT TGTTTGATCC ATCTAGAAAC ATGGCAAAAT ACCGCAATGT CTTGAACAGC 
CAAAATCTAC AGCCCCCAAT TATCCCACTA TTCCCTGTAA TCAAAAAGGA CCTTACATTT 
CTTCATGAAG GAAATGACTC CAAAGTAGAA GGTTTAGTCA ACTTCGAGAA ACTACGAATG 
ATTGCAAAAG AGATTCGCCA TGTGGGTCGA ATGGCTTCTG TTAACATGGA TCCTGCTTTA 
ATGTTCAGGA CTAGATCCCT CAGTCAAGGC AGTACAAATG CAGCAGTACT AGATGTTGCA 
CAAACAGGTG GTCATAAAAA GCGGGTCCGT CGCAGTTCCT TCCTCAATGC CAAAAAGCTG 
TATGAAGATG CCCAGATGGC TCGTAAAGTC AAGCAGTATC TCTCTAATTT GGACCTAGAG 
ATGGATGAAG AAAGCCTCCA GGCACTCTCC CTGCAGTGTG AACCAGCTAG CAATACATTA 
CCTAAGACCC CAGGGGAAAA GCGGTCTGGG AAACCTGAAA CATCACCAGT TGCACACAGG 
TCAGGGATCC AGCAAAAGGT GCAGCAGCAA CAGCAGCACA AAGCAAACCA AGCATTGCAA 
GTCCCTGCTG TGTCACTTTA CCCCTCTCGT AAGAAAGTGC CGGTCAAGGA CCTCCCGCCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAG AAAATACTTT CGTTGTCTGA AGAGGGAAGT 
ATAGACCGTC ATAAAAAGCA AGCTGAGGAC ACTATTTCAA ATGCCTCATC ACAGCTATCT 
TCTCCCCCCA CCTCCCCTCA GAGCTCTCCC CGGAAAGGTT TTACCTTAGC TGCCAGTGGA 
ACAGTGGATA ACTTTTCAGA CTCTGGTCAC AGTGAAATTT CCTCCAGATC TAGCATTGTC 
AGCAATTCTT CATTTGACTC TATGCCAGTA TCGCTACATG ATGAAAGGAG ACAGAGGCAC 
TCTGTCAGCA TTGTGGAGAC TAATCTTGGA GTGGGCAGAA TTGATAGAAG AGCAATTATT 
GAACCAGATC AGTACAGCCT AAGCTCCTAC GCACCACTGT CAGAGAACAG AGGTTTGTAT 
GCTGGAGCAA CCATGCTTTC TTCTCCCAGT ACGGAAGAGT TATCCCAGGA TCAGGGAGAT 
CGAGCATCAC TTGATGCAGC AGATAGTGGT CGTGGCAGTT GGACTTCTTG TTCAAGTGGT 
TCCCATGACA ACATACAGAC AATACAGCAC CAGCGAAGTT GGGAGACTCT TCCTTTTGGA 
CATGCTCATT TCGATAGTTC AGGCGATGGG GCAGGACTGT GGGCCTCAGG CAGCCACATG 
GACCCGATGA TGTTCTCCGA ACATGGCACA AAGTATGGCA GGCAGAGTCA AGGTAGGGAG 
GGCCTTGATC AAGCACAGTC CAGAGCAAGC TGGGCATCCT CCACAGGCTA CTGGGGAGAG 
GACTCTGAAG GTGATACAGG TACCATAAAA CGGAGGGGTG GGAAGGATGT GTCAATTGAA 
GCTGAAACCA GTAGCATAAC ATCAATAACA GCAGAGGATT CAAAGCCAGT TCCCATGCCA 
GCTCATATAG CTGTATCAAC AAGTAATGCA AAGGGTCTTA TTGCTCGAAA AGAGGGCCGC 
TATCGTGAAC CACCACCAAC CCCACCTGGT TACGTGGGAA TACCTATTGA CTTTGCAGAA 
GGAGTTTCCC ACCCAGTCCG AAAACCTCCA GATTACAACG TAGCACTTCA GAGGTCTAGG 
ATGGTGGCGC GGTCAAATGA AGCCCCTGGG ACTTCAGCTT CACATCAGCA AACACAGGGT 
CATTCATCTG GCAGGCCTGT TAATAAACCT CAGTGGCACA AACCAAACGA GTCAGACCCA 
CATCTTGCTC ACTATCAGTC TCAAGTGTTT TCCACAGAGG AGGATGAAGA TGAACAAGTT 
TCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020637079.1
CDS	567..5498
Translation	MASYVDNNFRQAVMKNPADRSPQDLEIVYSYLHGMEALSNLREHQLRMMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDAYPVGKPPLPRGYHTECTKQQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTPERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRMITLTKPSREAPLPFLLLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLARLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVTKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPSALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKSSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQALSLQCEPASNTLPKTPGEKRSGKPETSPVAHRSGIQQKVQQQQQHKANQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSIDRHKKQAEDTISNASSQLSSPPTSPQSSPRKGFTLAASGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRAIIEPDQYSLSSYAPLSENRGLYAGATMLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDPMMFSEHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSITAEDSKPVPMPAHIAVSTSNAKGLIARKEGRYREPPPTPPGYVGIPIDFAEGVSHPVRKPPDYNVALQRSRMVARSNEAPGTSASHQQTQGHSSGRPVNKPQWHKPNESDPHLAHYQSQVFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_020781420.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020781420.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGGAATG CACTAAACAG 
CAGCTTCCTG CAGATTTCAC AAAACTGCAC CTCACAGACA GTCTCCATCC ACAGGTGACC 
CACGTTTCAT CTAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG TAGCAGCCTA 
TCTGATATAT ACCAGGCAAC AGAAAGTGAA GCCGGTGACA TGGATCTAAG TGGTTTACCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ATATTGAGAG GGCATCTGAC 
CCTCTAATGA GCAGAGATAT TGTTAGAGAC TGCCTAGAAA AAGATCCAAT AGACAGGACA 
GATGATGATA TTGAACAACT CTTGGAATTC ATGCATCAGT TACCAGCTTT TGCTAATATG 
ACAATGTCTG TAAGGAGAGA ACTCTGTGCT GTAATGGTGT TTGCAGTTGT AGAAAGAGCA 
GGAACCATAG TTCTAAATGA TGGGGAAGAG CTGGACTCTT GGTCTGTTAT TTTGAATGGT 
TCTGTGGAAG TAACATATCC TGATGGAAGA ACAGAAATAT TATGCATGGG GAACAGTTTT 
GGTGTTTCTC CTACCATGGA CAAAGAGTAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GATTGCCAGT TTGTGTGCAT AGCTCAACAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAAAACATGC AGAAAGTAGA AGAGGAAGGA GAGATTGTTA TGGTAAAAGA GCATAGGGAA 
TTGGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTTATCA AGGGAACACC GGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCCGTA GTGGATCCAA CATTTATAGA GGATTTCCTT 
TTGACCTACA GGACCTTCCT TTCCAGTCCA ATGGAAGTGG GAAAGAAGTT ATTGGAGTGG 
TTCAATGATC CTAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAGGG AGATCCTGCA ATGACTCGTT TTTTAGAGGA ATTTGAGAAC 
AATTTGGAGA GAGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAACATAGC ATGTGCTGCT 
AAAGCTAAAC GGAGAATGAT AACCCTGACA AAGCCATCTC GGGAAGCCCC TTTGCCTTTC 
TTATTGTTGG GAGGATCTGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCCA CTGAGGCAGG CTTGAAACGT GGTGATCAGA TACTGGAAGT GAATGGCCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTCGAAATAA TACACATTTA 
TCTATCACTG TGAAAACCAA CCTGTTCGTT TTTAAAGAAC TTCTAGCAAG ATTATCCGAG 
GAGAAAAGAA ATGGTGCCCC TCATCTACCG AAAATTGGTG ATATTAAAAA AGCAAGCCGT 
TACTCCATTC CAGATCTTGC AGTTGATGTG GAACAGGTGA TAGGATTGGA AAAAGTAACC 
AAGAAAAGCA AAGCCAACAC TGTTGGTGGG CGAAACAAGT TAAAGAAGAT ACTAGACAAA 
ACCCGCATTA GTATCCTGCC TCAGAAACCA TACAATGATA TTGGAATTGG CCAGTCTCAG 
GATGACAGTA TTGTAGGACT GAGGCAGACC AAGCACATTC CTTCTGCTCT GCCAGTTAGT 
GGAACACTGT CCTCAAGCAA TCCTGACTTA TTGCAGTCAC ATCACCGCAT CTTAGATTTC 
AACACAACCC CAGATTTACC AGATCAGGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGC 
CGTTACATTA TGATCAGCAA GGACACAACT GCTAAGGAAG TGGTGATTCA GGCAATACGA 
GAATTTGCTC TAACTACCAC CCCAGATGCA TATTCTCTGT GTGAAGTCTC AGTCACTCCA 
GAGGGTGTCA TTAAACAACG GAGGCTCCCA GATCAACTCT CCAAGCTTGC TGATAGAATA 
CAACTTAGTG GAAGGTATTA TCTGAAGAAT AATATGGAAA CAGAAACATT GTGTTCGGAT 
GAAGATGCTC AGGAATTGCT GAGAGAGAGC CAAATTTCTC TACTGCAACT CAGTACTGTT 
GAGGTGGCTA CTCAGCTCTC AATGCGTAAC TTTGAATTGT TCCGGAACAT TGAACCTACA 
GAGTATATAG ATGACTTGTT TAAACTGAAA TCTAAATCTA GTTGTGCTAA CTTGAAAAAG 
TTTGAAGAAG TAATAAACCA GGAAACCTTT TGGGTAGCAT CTGAGATTCT GCGAGAAACC 
AACCAGCTGA AAAGGATGAA GATAATTAAG CACTTCATTA AGATAGCATT GCACTGCAGG 
GAATGCAAGA ACTTCAACTC CATGTTTGCA ATAATAAGTG GCCTGAATTT AGCTCCAGTT 
GCAAGACTAC GTACCACATG GGAAAAACTT CCAAGCAAAT ATGAAAAATT GTTTCAAGAT 
CTTCAGGATT TGTTTGATCC ATCTAGAAAC ATGGCAAAAT ACCGCAATGT CTTGAACAGC 
CAAAATCTAC AGCCCCCAAT TATCCCACTA TTCCCTGTAA TCAAAAAGGA CCTTACATTT 
CTTCATGAAG GAAATGACTC CAAAGTAGAA GGTTTAGTCA ACTTCGAGAA ACTACGAATG 
ATTGCAAAAG AGATTCGCCA TGTGGGTCGA ATGGCTTCTG TTAACATGGA TCCTGCTTTA 
ATGTTCAGGA CTAGATCCCT CAGTCAAGGC AGTACAAATG CAGCAGTACT AGATGTTGCA 
CAAACAGGTG GTCATAAAAA GCGGGTCCGT CGCAGTTCCT TCCTCAATGC CAAAAAGCTG 
TATGAAGATG CCCAGATGGC TCGTAAAGTC AAGCAGTATC TCTCTAATTT GGACCTAGAG 
ATGGATGAAG AAAGCCTCCA GGCACTCTCC CTGCAGTGTG AACCAGCTAG CAATACATTA 
CCTAAGACCC CAGGGGAAAA GCGGTCTGGG AAACCTGAAA CATCACCAGT TGCACACAGG 
TCAGGGATCC AGCAAAAGGT GCAGCAGCAA CAGCAGCACA AAGCAAACCA AGCATTGCAA 
GTCCCTGCTG TGTCACTTTA CCCCTCTCGT AAGAAAGTGC CGGTCAAGGA CCTCCCGCCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAG AAAATACTTT CGTTGTCTGA AGAGGGAAGT 
ATAGACCGTC ATAAAAAGCA AGCTGAGGAC ACTATTTCAA ATGCCTCATC ACAGCTATCT 
TCTCCCCCCA CCTCCCCTCA GAGCTCTCCC CGGAAAGGTT TTACCTTAGC TGCCAGTGGA 
ACAGTGGATA ACTTTTCAGA CTCTGGTCAC AGTGAAATTT CCTCCAGATC TAGCATTGTC 
AGCAATTCTT CATTTGACTC TATGCCAGTA TCGCTACATG ATGAAAGGAG ACAGAGGCAC 
TCTGTCAGCA TTGTGGAGAC TAATCTTGGA GTGGGCAGAA TTGATAGAAG AGCAATTATT 
GAACCAGATC AGTACAGCCT AAGCTCCTAC GCACCACTGT CAGAGAACAG AGGTTTGTAT 
GCTGGAGCAA CCATGCTTTC TTCTCCCAGT ACGGAAGAGT TATCCCAGGA TCAGGGAGAT 
CGAGCATCAC TTGATGCAGC AGATAGTGGT CGTGGCAGTT GGACTTCTTG TTCAAGTGGT 
TCCCATGACA ACATACAGAC AATACAGCAC CAGCGAAGTT GGGAGACTCT TCCTTTTGGA 
CATGCTCATT TCGATAGTTC AGGCGATGGG GCAGGACTGT GGGCCTCAGG CAGCCACATG 
GACCCGATGA TGTTCTCCGA ACATGGCACA AAGTATGGCA GGCAGAGTCA AGGTAGGGAG 
GGCCTTGATC AAGCACAGTC CAGAGCAAGC TGGGCATCCT CCACAGGCTA CTGGGGAGAG 
GACTCTGAAG GTGATACAGG TACCATAAAA CGGAGGGGTG GGAAGGATGT GTCAATTGAA 
GCTGAAACCA GTAGCATAAC ATCAATAACA GCAGAGGATT CAAAGCCAGT TCCCATGCCA 
GCTCATATAG CTGTATCAAC AAGTAATGCA AAGGGTCTTA TTGCTCGAAA AGAGGGCCGC 
TATCGTGAAC CACCACCAAC CCCACCTGGT TACGTGGGAA TACCTATTGA CTTTGCAGAA 
GGAGTTTCCC ACCCAGTCCG AAAACCTCCA GATTACAACG TAGCACTTCA GAGGTCTAGG 
ATGGTGGCGC GGTCAAATGA AGCCCCTGGG ACTTCAGCTT CACATCAGCA AACACAGGGT 
CATTCATCTG GCAGGCCTGT TAATAAACCT CAGTGGCACA AACCAAACGA GTCAGACCCA 
CATCTTGCTC ACTATCAGTC TCAAGTGTTT TCCACAGAGG AGGATGAAGA TGAACAAGTT 
TCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_020781421.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020637080.1 Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPo234408
Clone ID Related Accession (Same CDS sequence)	XM_020781421.1
Accession Version	XM_020781421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150727.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGGAATG CACTAAACAG 
CAGCTTCCTG CAGATTTCAC AAAACTGCAC CTCACAGACA GTCTCCATCC ACAGGTGACC 
CACGTTTCAT CTAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG TAGCAGCCTA 
TCTGATATAT ACCAGGCAAC AGAAAGTGAA GCCGGTGACA TGGATCTAAG TGGTTTACCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ATATTGAGAG GGCATCTGAC 
CCTCTAATGA GCAGAGATAT TGTTAGAGAC TGCCTAGAAA AAGATCCAAT AGACAGGACA 
GATGATGATA TTGAACAACT CTTGGAATTC ATGCATCAGT TACCAGCTTT TGCTAATATG 
ACAATGTCTG TAAGGAGAGA ACTCTGTGCT GTAATGGTGT TTGCAGTTGT AGAAAGAGCA 
GGAACCATAG TTCTAAATGA TGGGGAAGAG CTGGACTCTT GGTCTGTTAT TTTGAATGGT 
TCTGTGGAAG TAACATATCC TGATGGAAGA ACAGAAATAT TATGCATGGG GAACAGTTTT 
GGTGTTTCTC CTACCATGGA CAAAGAGTAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GATTGCCAGT TTGTGTGCAT AGCTCAACAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAAAACATGC AGAAAGTAGA AGAGGAAGGA GAGATTGTTA TGGTAAAAGA GCATAGGGAA 
TTGGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTTATCA AGGGAACACC GGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCCGTA GTGGATCCAA CATTTATAGA GGATTTCCTT 
TTGACCTACA GGACCTTCCT TTCCAGTCCA ATGGAAGTGG GAAAGAAGTT ATTGGAGTGG 
TTCAATGATC CTAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAGGG AGATCCTGCA ATGACTCGTT TTTTAGAGGA ATTTGAGAAC 
AATTTGGAGA GAGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAACATAGC ATGTGCTGCT 
AAAGCTAAAC GGAGAATGAT AACCCTGACA AAGCCATCTC GGGAAGCCCC TTTGCCTTTC 
TTATTGTTGG GAGGATCTGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCCA CTGAGGCAGG CTTGAAACGT GGTGATCAGA TACTGGAAGT GAATGGCCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTCGAAATAA TACACATTTA 
TCTATCACTG TGAAAACCAA CCTGTTCGTT TTTAAAGAAC TTCTAGCAAG ATTATCCGAG 
GAGAAAAGAA ATGGTGCCCC TCATCTACCG AAAATTGGTG ATATTAAAAA AGCAAGCCGT 
TACTCCATTC CAGATCTTGC AGTTGATGTG GAACAGGTGA TAGGATTGGA AAAAGTAACC 
AAGAAAAGCA AAGCCAACAC TGTTGGTGGG CGAAACAAGT TAAAGAAGAT ACTAGACAAA 
ACCCGCATTA GTATCCTGCC TCAGAAACCA TACAATGATA TTGGAATTGG CCAGTCTCAG 
GATGACAGTA TTGTAGGACT GAGGCAGACC AAGCACATTC CTTCTGCTCT GCCAGTTAGT 
GGAACACTGT CCTCAAGCAA TCCTGACTTA TTGCAGTCAC ATCACCGCAT CTTAGATTTC 
AACACAACCC CAGATTTACC AGATCAGGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGC 
CGTTACATTA TGATCAGCAA GGACACAACT GCTAAGGAAG TGGTGATTCA GGCAATACGA 
GAATTTGCTC TAACTACCAC CCCAGATGCA TATTCTCTGT GTGAAGTCTC AGTCACTCCA 
GAGGGTGTCA TTAAACAACG GAGGCTCCCA GATCAACTCT CCAAGCTTGC TGATAGAATA 
CAACTTAGTG GAAGGTATTA TCTGAAGAAT AATATGGAAA CAGAAACATT GTGTTCGGAT 
GAAGATGCTC AGGAATTGCT GAGAGAGAGC CAAATTTCTC TACTGCAACT CAGTACTGTT 
GAGGTGGCTA CTCAGCTCTC AATGCGTAAC TTTGAATTGT TCCGGAACAT TGAACCTACA 
GAGTATATAG ATGACTTGTT TAAACTGAAA TCTAAATCTA GTTGTGCTAA CTTGAAAAAG 
TTTGAAGAAG TAATAAACCA GGAAACCTTT TGGGTAGCAT CTGAGATTCT GCGAGAAACC 
AACCAGCTGA AAAGGATGAA GATAATTAAG CACTTCATTA AGATAGCATT GCACTGCAGG 
GAATGCAAGA ACTTCAACTC CATGTTTGCA ATAATAAGTG GCCTGAATTT AGCTCCAGTT 
GCAAGACTAC GTACCACATG GGAAAAACTT CCAAGCAAAT ATGAAAAATT GTTTCAAGAT 
CTTCAGGATT TGTTTGATCC ATCTAGAAAC ATGGCAAAAT ACCGCAATGT CTTGAACAGC 
CAAAATCTAC AGCCCCCAAT TATCCCACTA TTCCCTGTAA TCAAAAAGGA CCTTACATTT 
CTTCATGAAG GAAATGACTC CAAAGTAGAA GGTTTAGTCA ACTTCGAGAA ACTACGAATG 
ATTGCAAAAG AGATTCGCCA TGTGGGTCGA ATGGCTTCTG TTAACATGGA TCCTGCTTTA 
ATGTTCAGGA CTAGGAAGAA GAAGTGGAGG AGTCTGGGAT CCCTCAGTCA AGGCAGTACA 
AATGCAGCAG TACTAGATGT TGCACAAACA GGTGGTCATA AAAAGCGGGT CCGTCGCAGT 
TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGTAA AGTCAAGCAG 
TATCTCTCTA ATTTGGACCT AGAGATGGAT GAAGAAAGCC TCCAGGCACT CTCCCTGCAG 
TGTGAACCAG CTAGCAATAC ATTACCTAAG ACCCCAGGGG AAAAGCGGTC TGGGAAACCT 
GAAACATCAC CAGTTGCACA CAGGTCAGGG ATCCAGCAAA AGGTGCAGCA GCAACAGCAG 
CACAAAGCAA ACCAAGCATT GCAAGTCCCT GCTGTGTCAC TTTACCCCTC TCGTAAGAAA 
GTGCCGGTCA AGGACCTCCC GCCTTTTGGC ATAAACTCTC CACAAGCTTT AAAGAAAATA 
CTTTCGTTGT CTGAAGAGGG AAGTATAGAC CGTCATAAAA AGCAAGCTGA GGACACTATT 
TCAAATGCCT CATCACAGCT ATCTTCTCCC CCCACCTCCC CTCAGAGCTC TCCCCGGAAA 
GGTTTTACCT TAGCTGCCAG TGGAACAGTG GATAACTTTT CAGACTCTGG TCACAGTGAA 
ATTTCCTCCA GATCTAGCAT TGTCAGCAAT TCTTCATTTG ACTCTATGCC AGTATCGCTA 
CATGATGAAA GGAGACAGAG GCACTCTGTC AGCATTGTGG AGACTAATCT TGGAGTGGGC 
AGAATTGATA GAAGAGCAAT TATTGAACCA GATCAGTACA GCCTAAGCTC CTACGCACCA 
CTGTCAGAGA ACAGAGGTTT GTATGCTGGA GCAACCATGC TTTCTTCTCC CAGTACGGAA 
GAGTTATCCC AGGATCAGGG AGATCGAGCA TCACTTGATG CAGCAGATAG TGGTCGTGGC 
AGTTGGACTT CTTGTTCAAG TGGTTCCCAT GACAACATAC AGACAATACA GCACCAGCGA 
AGTTGGGAGA CTCTTCCTTT TGGACATGCT CATTTCGATA GTTCAGGCGA TGGGGCAGGA 
CTGTGGGCCT CAGGCAGCCA CATGGACCCG ATGATGTTCT CCGAACATGG CACAAAGTAT 
GGCAGGCAGA GTCAAGCTCG AAAAGAGGGC CGCTATCGTG AACCACCACC AACCCCACCT 
GGTTACGTGG GAATACCTAT TGACTTTGCA GAAGGAGTTT CCCACCCAGT CCGAAAACCT 
CCAGATTACA ACGTAGCACT TCAGAGGTCT AGGATGGTGG CGCGGTCAAA TGAAGCCCCT 
GGGACTTCAG CTTCACATCA GCAAACACAG GGTCATTCAT CTGGCAGGCC TGTTAATAAA 
CCTCAGTGGC ACAAACCAAA CGAGTCAGAC CCACATCTTG CTCACTATCA GTCTCAAGTG 
TTTTCCACAG AGGAGGATGA AGATGAACAA GTTTCTGCCG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020637080.1
CDS	567..5291
Translation	MASYVDNNFRQAVMKNPADRSPQDLEIVYSYLHGMEALSNLREHQLRMMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDAYPVGKPPLPRGYHTECTKQQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTPERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRMITLTKPSREAPLPFLLLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLARLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVTKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPSALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKSSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQALSLQCEPASNTLPKTPGEKRSGKPETSPVAHRSGIQQKVQQQQQHKANQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSIDRHKKQAEDTISNASSQLSSPPTSPQSSPRKGFTLAASGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRAIIEPDQYSLSSYAPLSENRGLYAGATMLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDPMMFSEHGTKYGRQSQARKEGRYREPPPTPPGYVGIPIDFAEGVSHPVRKPPDYNVALQRSRMVARSNEAPGTSASHQQTQGHSSGRPVNKPQWHKPNESDPHLAHYQSQVFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_020781421.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020781421.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAACTTCCGC CAGGCGGTCA TGAAGAACCC GGCCGACAGA 
AGCCCCCAGG ATCTGGAGAT AGTATATTCT TATTTACATG GAATGGAAGC TTTGTCTAAT 
TTGAGGGAAC ACCAGCTGAG AATGATGTGC GAAACAGTGA GATATGAGAG ACATGAAGCA 
AATGAAGTTC TGTACTACCC TGATGATATT GGGACCTGTT GGTATATTCT TTTGTCTGGT 
TCAGTTTTCA TCAAAGAATC AATGTTTCTT CCAAGAAGCA GTTTTGGCAA ACGTTCTGCA 
GGAAGCTTCA GACGTGGCTG TGAATGCATT GTGCTGGAGC CATCTGAGAT GATTGTGGTG 
GATTATATGG ATGAAAATGA AGAATATTTT CAGCGACAAG CATCACATAG GCAATCTCGA 
AGGAGATTTA GGAAAATCAA CCAAAAAGGA GAAAGACAAA CAATTATTGA TACTGTTGAT 
GCTTATCCTG TAGGAAAACC ACCTTTGCCT AGAGGTTATC ATACGGAATG CACTAAACAG 
CAGCTTCCTG CAGATTTCAC AAAACTGCAC CTCACAGACA GTCTCCATCC ACAGGTGACC 
CACGTTTCAT CTAGTCATTC AGGATGTAGC ATCACCAGTG ACTCTGGAAG TAGCAGCCTA 
TCTGATATAT ACCAGGCAAC AGAAAGTGAA GCCGGTGACA TGGATCTAAG TGGTTTACCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ATATTGAGAG GGCATCTGAC 
CCTCTAATGA GCAGAGATAT TGTTAGAGAC TGCCTAGAAA AAGATCCAAT AGACAGGACA 
GATGATGATA TTGAACAACT CTTGGAATTC ATGCATCAGT TACCAGCTTT TGCTAATATG 
ACAATGTCTG TAAGGAGAGA ACTCTGTGCT GTAATGGTGT TTGCAGTTGT AGAAAGAGCA 
GGAACCATAG TTCTAAATGA TGGGGAAGAG CTGGACTCTT GGTCTGTTAT TTTGAATGGT 
TCTGTGGAAG TAACATATCC TGATGGAAGA ACAGAAATAT TATGCATGGG GAACAGTTTT 
GGTGTTTCTC CTACCATGGA CAAAGAGTAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GATTGCCAGT TTGTGTGCAT AGCTCAACAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAAAACATGC AGAAAGTAGA AGAGGAAGGA GAGATTGTTA TGGTAAAAGA GCATAGGGAA 
TTGGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTTATCA AGGGAACACC GGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCCGTA GTGGATCCAA CATTTATAGA GGATTTCCTT 
TTGACCTACA GGACCTTCCT TTCCAGTCCA ATGGAAGTGG GAAAGAAGTT ATTGGAGTGG 
TTCAATGATC CTAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAGGG AGATCCTGCA ATGACTCGTT TTTTAGAGGA ATTTGAGAAC 
AATTTGGAGA GAGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAACATAGC ATGTGCTGCT 
AAAGCTAAAC GGAGAATGAT AACCCTGACA AAGCCATCTC GGGAAGCCCC TTTGCCTTTC 
TTATTGTTGG GAGGATCTGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCCA CTGAGGCAGG CTTGAAACGT GGTGATCAGA TACTGGAAGT GAATGGCCAA 
AACTTTGAAA ATATTCAGCT GTCAAAAGCC ATGGAAATTC TTCGAAATAA TACACATTTA 
TCTATCACTG TGAAAACCAA CCTGTTCGTT TTTAAAGAAC TTCTAGCAAG ATTATCCGAG 
GAGAAAAGAA ATGGTGCCCC TCATCTACCG AAAATTGGTG ATATTAAAAA AGCAAGCCGT 
TACTCCATTC CAGATCTTGC AGTTGATGTG GAACAGGTGA TAGGATTGGA AAAAGTAACC 
AAGAAAAGCA AAGCCAACAC TGTTGGTGGG CGAAACAAGT TAAAGAAGAT ACTAGACAAA 
ACCCGCATTA GTATCCTGCC TCAGAAACCA TACAATGATA TTGGAATTGG CCAGTCTCAG 
GATGACAGTA TTGTAGGACT GAGGCAGACC AAGCACATTC CTTCTGCTCT GCCAGTTAGT 
GGAACACTGT CCTCAAGCAA TCCTGACTTA TTGCAGTCAC ATCACCGCAT CTTAGATTTC 
AACACAACCC CAGATTTACC AGATCAGGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGC 
CGTTACATTA TGATCAGCAA GGACACAACT GCTAAGGAAG TGGTGATTCA GGCAATACGA 
GAATTTGCTC TAACTACCAC CCCAGATGCA TATTCTCTGT GTGAAGTCTC AGTCACTCCA 
GAGGGTGTCA TTAAACAACG GAGGCTCCCA GATCAACTCT CCAAGCTTGC TGATAGAATA 
CAACTTAGTG GAAGGTATTA TCTGAAGAAT AATATGGAAA CAGAAACATT GTGTTCGGAT 
GAAGATGCTC AGGAATTGCT GAGAGAGAGC CAAATTTCTC TACTGCAACT CAGTACTGTT 
GAGGTGGCTA CTCAGCTCTC AATGCGTAAC TTTGAATTGT TCCGGAACAT TGAACCTACA 
GAGTATATAG ATGACTTGTT TAAACTGAAA TCTAAATCTA GTTGTGCTAA CTTGAAAAAG 
TTTGAAGAAG TAATAAACCA GGAAACCTTT TGGGTAGCAT CTGAGATTCT GCGAGAAACC 
AACCAGCTGA AAAGGATGAA GATAATTAAG CACTTCATTA AGATAGCATT GCACTGCAGG 
GAATGCAAGA ACTTCAACTC CATGTTTGCA ATAATAAGTG GCCTGAATTT AGCTCCAGTT 
GCAAGACTAC GTACCACATG GGAAAAACTT CCAAGCAAAT ATGAAAAATT GTTTCAAGAT 
CTTCAGGATT TGTTTGATCC ATCTAGAAAC ATGGCAAAAT ACCGCAATGT CTTGAACAGC 
CAAAATCTAC AGCCCCCAAT TATCCCACTA TTCCCTGTAA TCAAAAAGGA CCTTACATTT 
CTTCATGAAG GAAATGACTC CAAAGTAGAA GGTTTAGTCA ACTTCGAGAA ACTACGAATG 
ATTGCAAAAG AGATTCGCCA TGTGGGTCGA ATGGCTTCTG TTAACATGGA TCCTGCTTTA 
ATGTTCAGGA CTAGGAAGAA GAAGTGGAGG AGTCTGGGAT CCCTCAGTCA AGGCAGTACA 
AATGCAGCAG TACTAGATGT TGCACAAACA GGTGGTCATA AAAAGCGGGT CCGTCGCAGT 
TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGTAA AGTCAAGCAG 
TATCTCTCTA ATTTGGACCT AGAGATGGAT GAAGAAAGCC TCCAGGCACT CTCCCTGCAG 
TGTGAACCAG CTAGCAATAC ATTACCTAAG ACCCCAGGGG AAAAGCGGTC TGGGAAACCT 
GAAACATCAC CAGTTGCACA CAGGTCAGGG ATCCAGCAAA AGGTGCAGCA GCAACAGCAG 
CACAAAGCAA ACCAAGCATT GCAAGTCCCT GCTGTGTCAC TTTACCCCTC TCGTAAGAAA 
GTGCCGGTCA AGGACCTCCC GCCTTTTGGC ATAAACTCTC CACAAGCTTT AAAGAAAATA 
CTTTCGTTGT CTGAAGAGGG AAGTATAGAC CGTCATAAAA AGCAAGCTGA GGACACTATT 
TCAAATGCCT CATCACAGCT ATCTTCTCCC CCCACCTCCC CTCAGAGCTC TCCCCGGAAA 
GGTTTTACCT TAGCTGCCAG TGGAACAGTG GATAACTTTT CAGACTCTGG TCACAGTGAA 
ATTTCCTCCA GATCTAGCAT TGTCAGCAAT TCTTCATTTG ACTCTATGCC AGTATCGCTA 
CATGATGAAA GGAGACAGAG GCACTCTGTC AGCATTGTGG AGACTAATCT TGGAGTGGGC 
AGAATTGATA GAAGAGCAAT TATTGAACCA GATCAGTACA GCCTAAGCTC CTACGCACCA 
CTGTCAGAGA ACAGAGGTTT GTATGCTGGA GCAACCATGC TTTCTTCTCC CAGTACGGAA 
GAGTTATCCC AGGATCAGGG AGATCGAGCA TCACTTGATG CAGCAGATAG TGGTCGTGGC 
AGTTGGACTT CTTGTTCAAG TGGTTCCCAT GACAACATAC AGACAATACA GCACCAGCGA 
AGTTGGGAGA CTCTTCCTTT TGGACATGCT CATTTCGATA GTTCAGGCGA TGGGGCAGGA 
CTGTGGGCCT CAGGCAGCCA CATGGACCCG ATGATGTTCT CCGAACATGG CACAAAGTAT 
GGCAGGCAGA GTCAAGCTCG AAAAGAGGGC CGCTATCGTG AACCACCACC AACCCCACCT 
GGTTACGTGG GAATACCTAT TGACTTTGCA GAAGGAGTTT CCCACCCAGT CCGAAAACCT 
CCAGATTACA ACGTAGCACT TCAGAGGTCT AGGATGGTGG CGCGGTCAAA TGAAGCCCCT 
GGGACTTCAG CTTCACATCA GCAAACACAG GGTCATTCAT CTGGCAGGCC TGTTAATAAA 
CCTCAGTGGC ACAAACCAAA CGAGTCAGAC CCACATCTTG CTCACTATCA GTCTCAAGTG 
TTTTCCACAG AGGAGGATGA AGATGAACAA GTTTCTGCCG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

RAPGEF2 cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム