GenCRISPR™ SaCas9 Antibody (11C12), MAb, Mouse

ELISA binding of GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) with recombinant Staphylococcus aureus CRISPR/Cas9 protein.
Coating antigen: SaCas9, 1 µg/ml.
SaCas9 antibody dilution start from 1000 ng/ml,EC₅₀= 3.75 ng/ml.

Standard curve of SaCas9 Sandwich ELISA. The SaCas9 Sandwich ELISA assay is developed by using GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) and GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) as capture and detection antibody, respectively. These two antibodies recognize different epitopes.
In this ELISA assay, GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) was labeled with Biotin. GenScript can provide customized conjugation service for this product per customer's request.
The sensitivity is ＜1 ng/ml and the detection range is 0-30 ng/ml.

Western Blot analysis of Hela transfected with various plasmids with GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40). The different Hela transfected with various plasmids indicate the minimum cross reaction of the antibody.
Predicted band size: 124 kDa
Loading:
Lane 1: 50μg Hela cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles)
Lane 2: 50μg Hela cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1)
Lane 3: 50μg Hela cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus)
Lane 4: 50μg Hela cell lysate transfected with AsCpf1(BB)-2A-GFP (U2UMQ6, Acidaminococcus sp. (strain BV3L6))
Lane 5: 50μg Hela cell lysate transfected with FnCpf1(BB)-2A-GFP (A0Q7Q2, Francisella tularensis subsp. novicida (strain U112))
Lane 6: 50μg Hela cell lysate transfected with LbCpf1(BB)-2A-GFP (A0A182DWE3, Lachnospiraceae bacterium ND2006)
Lane 7: 50μg Hela cell lysate (Non-transfected)
Primary Antibody:
GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
Secondary antibody：
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰], 0.125 μg/ml

GenCRISPR™ SaCas9 Antibody (11C12), mAb, Mouse

The product is specific for Staphylococcus aureus CRISPR/Cas9. This antibody binds with recombinant Staphylococcus aureus CRISPR/Cas9 protein in ELISA and Endogenous overexpressed Staphylococcus aureus CRISPR/Cas9 in immunofluorescence, western blot, immunoprecipitation.

Cat. No.	A01951
Size	40 μg : Quote Buy in bulk
Quantity
Price	¥17,089.00


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Overview

Specificity	The product is specific for Staphylococcus aureus CRISPR/Cas9. This antibody binds with recombinant Staphylococcus aureus CRISPR/Cas9 protein in ELISA and Endogenous overexpressed Staphylococcus aureus CRISPR/Cas9 in immunofluorescence, western blot, immunoprecipitation.
Host Species	Mouse
Immunogen	Recombinant Staphylococcus aureus CRISPR/Cas9

Properties

Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, contains 0.02% sodium azide. (Might be eligible for customized bulk orders.)
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Purification	Protein A affinity column
Clone ID	11C12

Examples

ELISA binding of GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) with recombinant Staphylococcus aureus CRISPR/Cas9 protein.
Coating antigen: SaCas9, 1 µg/ml.
SaCas9 antibody dilution start from 1000 ng/ml,EC₅₀= 3.75 ng/ml.

Standard curve of SaCas9 Sandwich ELISA. The SaCas9 Sandwich ELISA assay is developed by using GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) and GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) as capture and detection antibody, respectively. These two antibodies recognize different epitopes.
In this ELISA assay, GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) was labeled with Biotin. GenScript can provide customized conjugation service for this product per customer's request.
The sensitivity is ＜1 ng/ml and the detection range is 0-30 ng/ml.

Western Blot analysis of Hela transfected with various plasmids with GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40). The different Hela transfected with various plasmids indicate the minimum cross reaction of the antibody.
Predicted band size: 124 kDa
Loading:
Lane 1: 50μg Hela cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles)
Lane 2: 50μg Hela cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1)
Lane 3: 50μg Hela cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus)
Lane 4: 50μg Hela cell lysate transfected with AsCpf1(BB)-2A-GFP (U2UMQ6, Acidaminococcus sp. (strain BV3L6))
Lane 5: 50μg Hela cell lysate transfected with FnCpf1(BB)-2A-GFP (A0Q7Q2, Francisella tularensis subsp. novicida (strain U112))
Lane 6: 50μg Hela cell lysate transfected with LbCpf1(BB)-2A-GFP (A0A182DWE3, Lachnospiraceae bacterium ND2006)
Lane 7: 50μg Hela cell lysate (Non-transfected)
Primary Antibody:
GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
Secondary antibody：
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰], 0.125 μg/ml

Background

Target Background

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling researchers to precisely manipulate specific genomic elements, and facilitating the elucidation of target gene function in biology and diseases. CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The best characterized CRISPR-associated nucleases are the Cas9 proteins from Streptococcus pyogenes and Staphylococcus aureus. It is important that the precision of transfection and the level of Cas9 expression should be controlled strictly during the editing processes by using specific anti-CRISPR/Cas9 antibodies.

Synonyms

Mouse monoclonal to Staphylococcus aureus Cas9/SaCas9

GenCRISPR™ SaCas9 Antibody (11C12), mAb, Mouse

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