SARS-CoV-2

Human Cells

Expressed with the mutations of G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H.

Spike RBD (Arg319-Phe541) Accession # P0DTC2	Poly-His
N-term	C-term

HRP

SARS-CoV-2

293 Cells

Spike RBD (Arg319-Ser591) Accession # P0DTC2	Avi	Poly-His
N-term		C-term

SARS-CoV-2 SP RBD; 2019-nCoV SP RBD

His Tag Antibody Plate is a microtiter plate coated with THETM Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

SARS-CoV-2

CHO

Spike RBD [Arg319-Ser591 (L452R, T478K)] Accession # P0DTC2	Avi	Poly-His
N-term		C-term

Lineage B.1.617.2; VUI-21APR-02; variant Delta

FCM; ICC/IF;

Mouse IgG1,κ

PE

A synthetic peptide HHHHHH coupled to KLH

ELISA; WB; WB;

Rabbit IgG

Unconjugated

His epitope tag peptide HHHHHH conjugated to KLH

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins.

His Tag Antibody Plate is a microtiter plate coated with THETM Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

ICC/IF; FCM;

Mouse IgG1,κ

Fluorescein (FITC)

A synthetic peptide HHHHHH coupled to KLH

WB; FCM; ELISA;

Rabbit IgG, κ

Unconjugated

A synthetic peptide HHHHHH coupled to KLH