Protein Isolation and Analysis » His Tag ELISA Detection Kit

His Tag ELISA Detection Kit

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins. There are several potential usages:  Quickly identify the presence of His-tagged proteins in samples.  Optimize protein expression by monitoring the His-tagged proteins level.  High throughput screening of stable cell lines expressing His-tagged proteins.
L00436
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Description

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged_x000D_ proteins. There are several potential usages:

  1. Quickly identify the presence of His-tagged proteins in samples.
  2. Optimize protein expression by monitoring the His-tagged proteins level.
  3. High throughput screening of stable cell lines expressing His-tagged proteins.
This kit is based on competitive ELISA method. His Tag Plate in the kit is a 96-well microtiter_x000D_ plate coated with a His-tagged protein with the molecular weight of 12.7 kDa. The_x000D_ plate, which comprises of 8 wells x 12 strips, is demountable. When Anti-His Monoclonal_x000D_ Antibody and His-tagged proteins are added to the well , the coated His-tagged_x000D_ proteins compete with His Tag Standard (His-tagged protein with the molecular weight of 11.3_x000D_ kDa) in solution or His-tagged_x000D_ protein in sample to interact with Anti-His Monoclonal Antibody. The higher the_x000D_ concentration of the His-tagged protein in solution is, the less the antibody_x000D_ bound to the plate will be. The Anti-His Monoclonal Antibody used in the kit is_x000D_ a mouse anti His tag monoclonal antibody (GenScript A00186). Antibody Tracer, which is a horseradish peroxidase (HRP) conjugated Goat anti mouse IgG, is used for enzyme reaction. His-tagged protein, Anti-His Monoclonal Antibody and antibody tracer form a complex. Other unbound molecules can be removed by washing_x000D_ solution. The antibody tracer reacts with TMB substrate to develop blue_x000D_ product that turns yellow immediately when the Stop Solution is added, which_x000D_ can be measured by microplate reader at 450 nM.
In optimized_x000D_ test condition, each absorbance value is indicated to the individual His-tagged_x000D_ proteins amount in solution.  His-tagged_x000D_ protein standards of known concentration and the corresponding absorbance_x000D_ values are used to construct a standard curve. With the standard curve, His-tagged_x000D_ protein amount present in the unknown sample can be calculated by transforming_x000D_ its absorbance value. 
Note

For Research Use Only. Not for Use in Diagnostic Procedures. The operator should read technical manual carefully before using this product.

Overview
Key Features
FeaturesSpecifications
Sensitivity1 ng/ml His-tagged proteins
Detection Range1 ng/ml~729 ng/ml
Test SamplesN-terminal/C-terminal/internal His-tagged proteins.
4 x His/5 x His/6 x His-tagged proteins.
Mammalian, yeast and bacteria cell lysates or cell supernatant.
ConveniencyProvide all reagents required for test.
Complete test within 1.5 hours.
Reagent CompatibilityTolerable with various reagents at certain concentration (see Table 3)

Product Introduction
Product Information
ComponentQuantityPart. No
His Tag Plate1 plate (8 wells x 12 strips)436-80
Anti-His Monoclonal Antibody6 ml436-20
Antibody Tracer12 ml436-30
His Tag Standards
(0,1,3,9,27,81,243,729 ng/ml)		1 ml per each standard436-11,12,13,14,15,16,17,18
Assay Diluent60 ml436-60
20 × Wash Solution40 ml436-70
TMB Substrate12 ml436-40
Stop Solution6 ml436-50
His Tag Standard Stock (10 μg/ml)500 μl436-10
Plate Sealer2N/A
User Manual1N/A
Storage The unopened kit is stable for at least 12 months if stored at 2-8 °C and the opened kit may be stable for up to 1 month at 2-8 °C. Do not freeze the kit.