A 20 μl reaction in 1 × Cas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and eSpCas9-N-NLS for 2 hour at 37 °C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
GenCrispr eSpCas9-N-NLS Nuclease
Z03470 | |
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¥43,500.00 | |
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