リコンビナントタンパク質＿精製したリコンビナントタンパク質 » リコンビナントサイトカイン-免疫活性タンパク質 » TPO, His, Human
TPO, His,  Human

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5-5.0 ng/ml.

TPO, His,  Human

Lane 1: 3 μg of TPO, His, Human, non-reducing (NR)
Lane 2: 3 μg of TPO, His, Human, reducing (R)
> 95% as analyzed by SDS-PAGE

TPO, His,  Human

Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Z03434
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Product Introduction
Species Human
Protein Construction
Poly-His TPO (Ser22-Gly353)
Accession # P40225		 Poly-His
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by LAL method
Biological Activity Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.The ED50 for this effect is typically 0.5-5.0 ng/ml.
Expression System Human Cells
Apparent Molecular Weight 75~90 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. Avoid repeated freeze-thaw cycles.

Examples
  • TPO, His,  Human
    • TPO, His,  Human

    Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5-5.0 ng/ml.

  • TPO, His,  Human
    • TPO, His,  Human

    Lane 1: 3 μg of TPO, His, Human, non-reducing (NR)
    Lane 2: 3 μg of TPO, His, Human, reducing (R)
    > 95% as analyzed by SDS-PAGE


Background
Target Background Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Synonyms Thrombopoietin; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growthand development factor; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF; MKCSF; ML; MPLLG; THCYT1; TPO

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.