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TNF-α (80-235aa), Mouse

ED50 < 0.05 ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells, corresponding to a specific activity of > 2.0 × 107 units/mg.

TNF-α (80-235aa), Mouse

Lane 1: 2 μg of TNF-α (80-235aa), Mouse, non-reducing (NR)
Lane 2: 2 μg of TNF-α (80-235aa), Mouse, reducing (R)
> 98% as analyzed by SDS-PAGE

TNF-α (80-235aa), Mouse

Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseasesuch as Crohn's disease, rheumatoid arthritis and graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Z03333
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Product Introduction
Species Mouse
Protein Construction
TNF-α (Leu80-Leu235)
Accession # P06804
Purity > 98% as analyzed by SDS-PAGE
> 98% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.05 ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells, corresponding to a specific activity of > 2.0 × 107 units/mg.
Expression System E. coli
Apparent Molecular Weight ~17.3 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • TNF-α (80-235aa), Mouse
    • TNF-α (80-235aa), Mouse

    ED50 < 0.05 ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells, corresponding to a specific activity of > 2.0 × 107 units/mg.

  • TNF-α (80-235aa), Mouse
    • TNF-α (80-235aa), Mouse

    Lane 1: 2 μg of TNF-α (80-235aa), Mouse, non-reducing (NR)
    Lane 2: 2 μg of TNF-α (80-235aa), Mouse, reducing (R)
    > 98% as analyzed by SDS-PAGE


Background
Target Background Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseasesuch as Crohn's disease, rheumatoid arthritis and graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Synonyms TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.