LOC105221468 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105221468
Entrez Gene ID	105221468
Full Name	probable 28S rRNA (cytosine-C(5))-methyltransferase
Synonyms	NOP2_1
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011198496.2	XP_011196798.1	probable 28S rRNA (cytosine-C(5))-methyltransferase
XM_011198496.1	XP_011196798.1	probable 28S rRNA (cytosine-C(5))-methyltransferase

Pathways from BioSystems
Gene Ontology

The following LOC105221468 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105221468 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa193765	XM_011198496.2 Latest version!	Zeugodacus cucurbitae probable 28S rRNA (cytosine-C(5))-methyltransferase (LOC105221468), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBa193765	XM_011198496.1	Bactrocera cucurbitae probable 28S rRNA (cytosine-C(5))-methyltransferase (LOC105221468), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105221468 ( XM_011198496.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196798.1 Zeugodacus cucurbitae probable 28S rRNA (cytosine-C(5))-methyltransferase (LOC105221468), mRNA.

Price & Availability↑

CloneID	OBa193765
Clone ID Related Accession (Same CDS sequence)	XM_011198496.2 , XM_011198496.1
Accession Version	XM_011198496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable 28S rRNA (cytosine-C(5))-methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011198496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGGA AAGCAGAATT TAGTGAGAAG CCAAAAAAAG GTCCTGGTCG GAAGGCTCGC 
AAGCAAAAGC CACCTACATT CCCGAAAAAA TCGTTCGCAC CTTCAGATGA TAAGGATGAT 
AAAAAACTAT CCCATCGCCA AAAGCAACGC TTGGTTAAGC GTGATCAGAA AAAAGATATC 
CAAAAAATAA AAAAACAAAA ATTCAATATG AAGTTAAGCC AAGAAAATAA TGGTAAATCT 
AAAGATAGGA GGAAGCAATA TAGTGACGGT GACGATATAA TCGACGATGT TAAAATTCAA 
AATAAATGTG ACACAGAAGA ACTAGATGGA AAAGTGGGCA TCCGGCAGGA TTTAAAATCT 
CGGAAAAAAA AGTTGTTGGG TTTTACTGAT GATAATCAAG ATTGGCTGAA ACCAAAGCAA 
TCGAAAAAAT TAAAGAGATT AAAAAGTATA GACGATCAAG AAATGAACAA TGGTGAAGAT 
GAAAAAGATT TATGGGAAGG ACAAACCGAA AGCGGGAGGG AACAGAATGA CGATGAGGAT 
GCTAGTAAAG AAGCTCTTAA AGTTGAAAAT CTAGATGATA TATCAGATTA TGATGATGAA 
AATGATAATT CAGATGACGA CTTTGAATTA TCTGATGAAG AGGGTAATGA TACTGATTCT 
ACCAATAGCG AATCCGATGA TAATGAAGCT GATGATACTC TATTACCGAT CGAACGGGAA 
AATAAGAAGT TAAGGAAACG TGAAGCCAAA GAAGCTAAAA TGGGTACTCA GGAAATGCAA 
ATGTCTATTG ATCGGCAGGA GGTTTTCAAA TTCCCAGAGA GAGATGAAGA AAACCAAAAG 
GAAATAACAC TTCAGGAAAT ACAACAACGT ATTAAGGATA TTACTCTTGT ATTATCTGAT 
TTCAAAAAGT ACCGTCAAGA AGATCATTCT CGACAAGAAT ATTTAGATCT ATTGCGACAT 
GATCTTTGCA TCTACTATAG CTATAATGAT TTTCTTATGG GAAAATTAAT GGATATTTTC 
CCATTGACAG AATTAATGGA GTATTTAGAA ACATCAGAAG TTCCTCGTCC TCTTACAATT 
CGTACGAATT CATTAAAAAC ACGTAGAAGA GATTTGGCGG CAGCATTAAT TAACCGAGGC 
GTTAACTTAG ATCCTTTGGG TAAATGGACT CAAGTCGGTT TAGTCGTTTA TAATTCGCAA 
GTTCCACTTG GGGCTACTCC CGAATACTTA GCTGGTCACT ATATGATTCA AGGTGCATCC 
TCAATGCTAC CAGTTATGGC TTTAGCACCG CAGGAGAGTG AACGTATTTT AGATATGTGT 
TCTGCACCAG GCGGCAAGGG ATCACATATT GCTGCGATTA TGAAGAATAC AGGCGTTCTT 
TTTGCTAATG ATGCCAATAG AGAGCGGATA AAAGCAGTAG TGGCCAATTT TCATCGCCTT 
GGCGTTATTA ATGCTATTAT TAGCTGTGAA GATGGCTGCA AATTTCGTAA TGTAATGTCA 
GGATTTGATC GTATTCTCTT AGATGCACCG TGCACAGGCA CAGGTGTTGT GGCGAAAGAC 
CCAAGCGTCA AATCAACAAA GAATGAGGTC GATGTACAGC GTTGTTACAA TCTTCAACGC 
AAGTTGTTGT TAACAGCCAT TGATTGTGTA GATGCTAAAT CTAAAACCGG TGGATACATT 
GTGTATTCAA CATGTTCGGT ATTACCTGAG GAGAATGAAT GGGTTATAGA CTATGCCCTC 
AAAAAACGAA ATGTTAAGCT TGTTCCAACT GGTTTAGATT TTGGTGTTGA AGGTTTCACA 
AAATATCGTC AACATCGCTT CCATCCCAGT TTAAATTTGA CTCGCCGTTA TTATCCCCAT 
GTACATAATA TGGATGGATT TTATGTTGCA AAATTGAAAA AGTTCTCAAA CACTATACCG 
ATGACTAAGG AACAGCAAGA AGCAGATGAA AAAGATTTGG ATGACGCGAT AGCGAATGCT 
AAATCAATAG AGAATGAGAA TTCCAACATT AGCGAACAGT TGGAATGTGA AGAGACGCGG 
CCCCGGAAAC CACTCGGAAA ACACGCTGGA AAGCCAGACT TGTCGGAAGT AGAATTGGAC 
TTAAAGAAGA GAAAATTGCA ACAGAATAAG AATAAATATA TTGCTAATGT TTTTGAAAAA 
CCATTAAAAG TAAATAAGAA TGAAATGGAA GTGAAACCAG AAAATCAAAC TACAACAGTT 
GAGAAGCAAT TAAATGTGGT TGAATTAAAA AATAGTAAAA ATCATGGAGC ACAAATTGGT 
TCTGAATGCA AAAAGGTACA ACGGAAGTTG ATTACAAAAA AACAGAAAGA ACTCCCTCAA 
TTGTTAGAGG GAAAGTCATT TGCAGGACAA AATAAATTGA AGCAGAAATC GAAACTGATT 
GGTCAATTGA AAAAAGACAA ATCCATGAAA AAGCACAAAA ATACAATTTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196798.1
CDS	173..2626
Translation	MGRKAEFSEKPKKGPGRKARKQKPPTFPKKSFAPSDDKDDKKLSHRQKQRLVKRDQKKDIQKIKKQKFNMKLSQENNGKSKDRRKQYSDGDDIIDDVKIQNKCDTEELDGKVGIRQDLKSRKKKLLGFTDDNQDWLKPKQSKKLKRLKSIDDQEMNNGEDEKDLWEGQTESGREQNDDEDASKEALKVENLDDISDYDDENDNSDDDFELSDEEGNDTDSTNSESDDNEADDTLLPIERENKKLRKREAKEAKMGTQEMQMSIDRQEVFKFPERDEENQKEITLQEIQQRIKDITLVLSDFKKYRQEDHSRQEYLDLLRHDLCIYYSYNDFLMGKLMDIFPLTELMEYLETSEVPRPLTIRTNSLKTRRRDLAAALINRGVNLDPLGKWTQVGLVVYNSQVPLGATPEYLAGHYMIQGASSMLPVMALAPQESERILDMCSAPGGKGSHIAAIMKNTGVLFANDANRERIKAVVANFHRLGVINAIISCEDGCKFRNVMSGFDRILLDAPCTGTGVVAKDPSVKSTKNEVDVQRCYNLQRKLLLTAIDCVDAKSKTGGYIVYSTCSVLPEENEWVIDYALKKRNVKLVPTGLDFGVEGFTKYRQHRFHPSLNLTRRYYPHVHNMDGFYVAKLKKFSNTIPMTKEQQEADEKDLDDAIANAKSIENENSNISEQLECEETRPRKPLGKHAGKPDLSEVELDLKKRKLQQNKNKYIANVFEKPLKVNKNEMEVKPENQTTTVEKQLNVVELKNSKNHGAQIGSECKKVQRKLITKKQKELPQLLEGKSFAGQNKLKQKSKLIGQLKKDKSMKKHKNTIC

Target ORF information:

RefSeq Version	XM_011198496.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae probable 28S rRNA (cytosine-C(5))-methyltransferase (LOC105221468), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011198496.2

ORF Insert Sequence:

ATGGGACGGA AAGCAGAATT TAGTGAGAAG CCAAAAAAAG GTCCTGGTCG GAAGGCTCGC 
AAGCAAAAGC CACCTACATT CCCGAAAAAA TCGTTCGCAC CTTCAGATGA TAAGGATGAT 
AAAAAACTAT CCCATCGCCA AAAGCAACGC TTGGTTAAGC GTGATCAGAA AAAAGATATC 
CAAAAAATAA AAAAACAAAA ATTCAATATG AAGTTAAGCC AAGAAAATAA TGGTAAATCT 
AAAGATAGGA GGAAGCAATA TAGTGACGGT GACGATATAA TCGACGATGT TAAAATTCAA 
AATAAATGTG ACACAGAAGA ACTAGATGGA AAAGTGGGCA TCCGGCAGGA TTTAAAATCT 
CGGAAAAAAA AGTTGTTGGG TTTTACTGAT GATAATCAAG ATTGGCTGAA ACCAAAGCAA 
TCGAAAAAAT TAAAGAGATT AAAAAGTATA GACGATCAAG AAATGAACAA TGGTGAAGAT 
GAAAAAGATT TATGGGAAGG ACAAACCGAA AGCGGGAGGG AACAGAATGA CGATGAGGAT 
GCTAGTAAAG AAGCTCTTAA AGTTGAAAAT CTAGATGATA TATCAGATTA TGATGATGAA 
AATGATAATT CAGATGACGA CTTTGAATTA TCTGATGAAG AGGGTAATGA TACTGATTCT 
ACCAATAGCG AATCCGATGA TAATGAAGCT GATGATACTC TATTACCGAT CGAACGGGAA 
AATAAGAAGT TAAGGAAACG TGAAGCCAAA GAAGCTAAAA TGGGTACTCA GGAAATGCAA 
ATGTCTATTG ATCGGCAGGA GGTTTTCAAA TTCCCAGAGA GAGATGAAGA AAACCAAAAG 
GAAATAACAC TTCAGGAAAT ACAACAACGT ATTAAGGATA TTACTCTTGT ATTATCTGAT 
TTCAAAAAGT ACCGTCAAGA AGATCATTCT CGACAAGAAT ATTTAGATCT ATTGCGACAT 
GATCTTTGCA TCTACTATAG CTATAATGAT TTTCTTATGG GAAAATTAAT GGATATTTTC 
CCATTGACAG AATTAATGGA GTATTTAGAA ACATCAGAAG TTCCTCGTCC TCTTACAATT 
CGTACGAATT CATTAAAAAC ACGTAGAAGA GATTTGGCGG CAGCATTAAT TAACCGAGGC 
GTTAACTTAG ATCCTTTGGG TAAATGGACT CAAGTCGGTT TAGTCGTTTA TAATTCGCAA 
GTTCCACTTG GGGCTACTCC CGAATACTTA GCTGGTCACT ATATGATTCA AGGTGCATCC 
TCAATGCTAC CAGTTATGGC TTTAGCACCG CAGGAGAGTG AACGTATTTT AGATATGTGT 
TCTGCACCAG GCGGCAAGGG ATCACATATT GCTGCGATTA TGAAGAATAC AGGCGTTCTT 
TTTGCTAATG ATGCCAATAG AGAGCGGATA AAAGCAGTAG TGGCCAATTT TCATCGCCTT 
GGCGTTATTA ATGCTATTAT TAGCTGTGAA GATGGCTGCA AATTTCGTAA TGTAATGTCA 
GGATTTGATC GTATTCTCTT AGATGCACCG TGCACAGGCA CAGGTGTTGT GGCGAAAGAC 
CCAAGCGTCA AATCAACAAA GAATGAGGTC GATGTACAGC GTTGTTACAA TCTTCAACGC 
AAGTTGTTGT TAACAGCCAT TGATTGTGTA GATGCTAAAT CTAAAACCGG TGGATACATT 
GTGTATTCAA CATGTTCGGT ATTACCTGAG GAGAATGAAT GGGTTATAGA CTATGCCCTC 
AAAAAACGAA ATGTTAAGCT TGTTCCAACT GGTTTAGATT TTGGTGTTGA AGGTTTCACA 
AAATATCGTC AACATCGCTT CCATCCCAGT TTAAATTTGA CTCGCCGTTA TTATCCCCAT 
GTACATAATA TGGATGGATT TTATGTTGCA AAATTGAAAA AGTTCTCAAA CACTATACCG 
ATGACTAAGG AACAGCAAGA AGCAGATGAA AAAGATTTGG ATGACGCGAT AGCGAATGCT 
AAATCAATAG AGAATGAGAA TTCCAACATT AGCGAACAGT TGGAATGTGA AGAGACGCGG 
CCCCGGAAAC CACTCGGAAA ACACGCTGGA AAGCCAGACT TGTCGGAAGT AGAATTGGAC 
TTAAAGAAGA GAAAATTGCA ACAGAATAAG AATAAATATA TTGCTAATGT TTTTGAAAAA 
CCATTAAAAG TAAATAAGAA TGAAATGGAA GTGAAACCAG AAAATCAAAC TACAACAGTT 
GAGAAGCAAT TAAATGTGGT TGAATTAAAA AATAGTAAAA ATCATGGAGC ACAAATTGGT 
TCTGAATGCA AAAAGGTACA ACGGAAGTTG ATTACAAAAA AACAGAAAGA ACTCCCTCAA 
TTGTTAGAGG GAAAGTCATT TGCAGGACAA AATAAATTGA AGCAGAAATC GAAACTGATT 
GGTCAATTGA AAAAAGACAA ATCCATGAAA AAGCACAAAA ATACAATTTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221468 ( XM_011198496.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196798.1 Bactrocera cucurbitae probable 28S rRNA (cytosine-C(5))-methyltransferase (LOC105221468), mRNA.

Price & Availability↑

CloneID	OBa193765
Clone ID Related Accession (Same CDS sequence)	XM_011198496.2 , XM_011198496.1
Accession Version	XM_011198496.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable 28S rRNA (cytosine-C(5))-methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGGA AAGCAGAATT TAGTGAGAAG CCAAAAAAAG GTCCTGGTCG GAAGGCTCGC 
AAGCAAAAGC CACCTACATT CCCGAAAAAA TCGTTCGCAC CTTCAGATGA TAAGGATGAT 
AAAAAACTAT CCCATCGCCA AAAGCAACGC TTGGTTAAGC GTGATCAGAA AAAAGATATC 
CAAAAAATAA AAAAACAAAA ATTCAATATG AAGTTAAGCC AAGAAAATAA TGGTAAATCT 
AAAGATAGGA GGAAGCAATA TAGTGACGGT GACGATATAA TCGACGATGT TAAAATTCAA 
AATAAATGTG ACACAGAAGA ACTAGATGGA AAAGTGGGCA TCCGGCAGGA TTTAAAATCT 
CGGAAAAAAA AGTTGTTGGG TTTTACTGAT GATAATCAAG ATTGGCTGAA ACCAAAGCAA 
TCGAAAAAAT TAAAGAGATT AAAAAGTATA GACGATCAAG AAATGAACAA TGGTGAAGAT 
GAAAAAGATT TATGGGAAGG ACAAACCGAA AGCGGGAGGG AACAGAATGA CGATGAGGAT 
GCTAGTAAAG AAGCTCTTAA AGTTGAAAAT CTAGATGATA TATCAGATTA TGATGATGAA 
AATGATAATT CAGATGACGA CTTTGAATTA TCTGATGAAG AGGGTAATGA TACTGATTCT 
ACCAATAGCG AATCCGATGA TAATGAAGCT GATGATACTC TATTACCGAT CGAACGGGAA 
AATAAGAAGT TAAGGAAACG TGAAGCCAAA GAAGCTAAAA TGGGTACTCA GGAAATGCAA 
ATGTCTATTG ATCGGCAGGA GGTTTTCAAA TTCCCAGAGA GAGATGAAGA AAACCAAAAG 
GAAATAACAC TTCAGGAAAT ACAACAACGT ATTAAGGATA TTACTCTTGT ATTATCTGAT 
TTCAAAAAGT ACCGTCAAGA AGATCATTCT CGACAAGAAT ATTTAGATCT ATTGCGACAT 
GATCTTTGCA TCTACTATAG CTATAATGAT TTTCTTATGG GAAAATTAAT GGATATTTTC 
CCATTGACAG AATTAATGGA GTATTTAGAA ACATCAGAAG TTCCTCGTCC TCTTACAATT 
CGTACGAATT CATTAAAAAC ACGTAGAAGA GATTTGGCGG CAGCATTAAT TAACCGAGGC 
GTTAACTTAG ATCCTTTGGG TAAATGGACT CAAGTCGGTT TAGTCGTTTA TAATTCGCAA 
GTTCCACTTG GGGCTACTCC CGAATACTTA GCTGGTCACT ATATGATTCA AGGTGCATCC 
TCAATGCTAC CAGTTATGGC TTTAGCACCG CAGGAGAGTG AACGTATTTT AGATATGTGT 
TCTGCACCAG GCGGCAAGGG ATCACATATT GCTGCGATTA TGAAGAATAC AGGCGTTCTT 
TTTGCTAATG ATGCCAATAG AGAGCGGATA AAAGCAGTAG TGGCCAATTT TCATCGCCTT 
GGCGTTATTA ATGCTATTAT TAGCTGTGAA GATGGCTGCA AATTTCGTAA TGTAATGTCA 
GGATTTGATC GTATTCTCTT AGATGCACCG TGCACAGGCA CAGGTGTTGT GGCGAAAGAC 
CCAAGCGTCA AATCAACAAA GAATGAGGTC GATGTACAGC GTTGTTACAA TCTTCAACGC 
AAGTTGTTGT TAACAGCCAT TGATTGTGTA GATGCTAAAT CTAAAACCGG TGGATACATT 
GTGTATTCAA CATGTTCGGT ATTACCTGAG GAGAATGAAT GGGTTATAGA CTATGCCCTC 
AAAAAACGAA ATGTTAAGCT TGTTCCAACT GGTTTAGATT TTGGTGTTGA AGGTTTCACA 
AAATATCGTC AACATCGCTT CCATCCCAGT TTAAATTTGA CTCGCCGTTA TTATCCCCAT 
GTACATAATA TGGATGGATT TTATGTTGCA AAATTGAAAA AGTTCTCAAA CACTATACCG 
ATGACTAAGG AACAGCAAGA AGCAGATGAA AAAGATTTGG ATGACGCGAT AGCGAATGCT 
AAATCAATAG AGAATGAGAA TTCCAACATT AGCGAACAGT TGGAATGTGA AGAGACGCGG 
CCCCGGAAAC CACTCGGAAA ACACGCTGGA AAGCCAGACT TGTCGGAAGT AGAATTGGAC 
TTAAAGAAGA GAAAATTGCA ACAGAATAAG AATAAATATA TTGCTAATGT TTTTGAAAAA 
CCATTAAAAG TAAATAAGAA TGAAATGGAA GTGAAACCAG AAAATCAAAC TACAACAGTT 
GAGAAGCAAT TAAATGTGGT TGAATTAAAA AATAGTAAAA ATCATGGAGC ACAAATTGGT 
TCTGAATGCA AAAAGGTACA ACGGAAGTTG ATTACAAAAA AACAGAAAGA ACTCCCTCAA 
TTGTTAGAGG GAAAGTCATT TGCAGGACAA AATAAATTGA AGCAGAAATC GAAACTGATT 
GGTCAATTGA AAAAAGACAA ATCCATGAAA AAGCACAAAA ATACAATTTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196798.1
CDS	101..2554
Translation	MGRKAEFSEKPKKGPGRKARKQKPPTFPKKSFAPSDDKDDKKLSHRQKQRLVKRDQKKDIQKIKKQKFNMKLSQENNGKSKDRRKQYSDGDDIIDDVKIQNKCDTEELDGKVGIRQDLKSRKKKLLGFTDDNQDWLKPKQSKKLKRLKSIDDQEMNNGEDEKDLWEGQTESGREQNDDEDASKEALKVENLDDISDYDDENDNSDDDFELSDEEGNDTDSTNSESDDNEADDTLLPIERENKKLRKREAKEAKMGTQEMQMSIDRQEVFKFPERDEENQKEITLQEIQQRIKDITLVLSDFKKYRQEDHSRQEYLDLLRHDLCIYYSYNDFLMGKLMDIFPLTELMEYLETSEVPRPLTIRTNSLKTRRRDLAAALINRGVNLDPLGKWTQVGLVVYNSQVPLGATPEYLAGHYMIQGASSMLPVMALAPQESERILDMCSAPGGKGSHIAAIMKNTGVLFANDANRERIKAVVANFHRLGVINAIISCEDGCKFRNVMSGFDRILLDAPCTGTGVVAKDPSVKSTKNEVDVQRCYNLQRKLLLTAIDCVDAKSKTGGYIVYSTCSVLPEENEWVIDYALKKRNVKLVPTGLDFGVEGFTKYRQHRFHPSLNLTRRYYPHVHNMDGFYVAKLKKFSNTIPMTKEQQEADEKDLDDAIANAKSIENENSNISEQLECEETRPRKPLGKHAGKPDLSEVELDLKKRKLQQNKNKYIANVFEKPLKVNKNEMEVKPENQTTTVEKQLNVVELKNSKNHGAQIGSECKKVQRKLITKKQKELPQLLEGKSFAGQNKLKQKSKLIGQLKKDKSMKKHKNTIC

Target ORF information:

RefSeq Version	XM_011198496.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable 28S rRNA (cytosine-C(5))-methyltransferase (LOC105221468), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011198496.1

ORF Insert Sequence:

ATGGGACGGA AAGCAGAATT TAGTGAGAAG CCAAAAAAAG GTCCTGGTCG GAAGGCTCGC 
AAGCAAAAGC CACCTACATT CCCGAAAAAA TCGTTCGCAC CTTCAGATGA TAAGGATGAT 
AAAAAACTAT CCCATCGCCA AAAGCAACGC TTGGTTAAGC GTGATCAGAA AAAAGATATC 
CAAAAAATAA AAAAACAAAA ATTCAATATG AAGTTAAGCC AAGAAAATAA TGGTAAATCT 
AAAGATAGGA GGAAGCAATA TAGTGACGGT GACGATATAA TCGACGATGT TAAAATTCAA 
AATAAATGTG ACACAGAAGA ACTAGATGGA AAAGTGGGCA TCCGGCAGGA TTTAAAATCT 
CGGAAAAAAA AGTTGTTGGG TTTTACTGAT GATAATCAAG ATTGGCTGAA ACCAAAGCAA 
TCGAAAAAAT TAAAGAGATT AAAAAGTATA GACGATCAAG AAATGAACAA TGGTGAAGAT 
GAAAAAGATT TATGGGAAGG ACAAACCGAA AGCGGGAGGG AACAGAATGA CGATGAGGAT 
GCTAGTAAAG AAGCTCTTAA AGTTGAAAAT CTAGATGATA TATCAGATTA TGATGATGAA 
AATGATAATT CAGATGACGA CTTTGAATTA TCTGATGAAG AGGGTAATGA TACTGATTCT 
ACCAATAGCG AATCCGATGA TAATGAAGCT GATGATACTC TATTACCGAT CGAACGGGAA 
AATAAGAAGT TAAGGAAACG TGAAGCCAAA GAAGCTAAAA TGGGTACTCA GGAAATGCAA 
ATGTCTATTG ATCGGCAGGA GGTTTTCAAA TTCCCAGAGA GAGATGAAGA AAACCAAAAG 
GAAATAACAC TTCAGGAAAT ACAACAACGT ATTAAGGATA TTACTCTTGT ATTATCTGAT 
TTCAAAAAGT ACCGTCAAGA AGATCATTCT CGACAAGAAT ATTTAGATCT ATTGCGACAT 
GATCTTTGCA TCTACTATAG CTATAATGAT TTTCTTATGG GAAAATTAAT GGATATTTTC 
CCATTGACAG AATTAATGGA GTATTTAGAA ACATCAGAAG TTCCTCGTCC TCTTACAATT 
CGTACGAATT CATTAAAAAC ACGTAGAAGA GATTTGGCGG CAGCATTAAT TAACCGAGGC 
GTTAACTTAG ATCCTTTGGG TAAATGGACT CAAGTCGGTT TAGTCGTTTA TAATTCGCAA 
GTTCCACTTG GGGCTACTCC CGAATACTTA GCTGGTCACT ATATGATTCA AGGTGCATCC 
TCAATGCTAC CAGTTATGGC TTTAGCACCG CAGGAGAGTG AACGTATTTT AGATATGTGT 
TCTGCACCAG GCGGCAAGGG ATCACATATT GCTGCGATTA TGAAGAATAC AGGCGTTCTT 
TTTGCTAATG ATGCCAATAG AGAGCGGATA AAAGCAGTAG TGGCCAATTT TCATCGCCTT 
GGCGTTATTA ATGCTATTAT TAGCTGTGAA GATGGCTGCA AATTTCGTAA TGTAATGTCA 
GGATTTGATC GTATTCTCTT AGATGCACCG TGCACAGGCA CAGGTGTTGT GGCGAAAGAC 
CCAAGCGTCA AATCAACAAA GAATGAGGTC GATGTACAGC GTTGTTACAA TCTTCAACGC 
AAGTTGTTGT TAACAGCCAT TGATTGTGTA GATGCTAAAT CTAAAACCGG TGGATACATT 
GTGTATTCAA CATGTTCGGT ATTACCTGAG GAGAATGAAT GGGTTATAGA CTATGCCCTC 
AAAAAACGAA ATGTTAAGCT TGTTCCAACT GGTTTAGATT TTGGTGTTGA AGGTTTCACA 
AAATATCGTC AACATCGCTT CCATCCCAGT TTAAATTTGA CTCGCCGTTA TTATCCCCAT 
GTACATAATA TGGATGGATT TTATGTTGCA AAATTGAAAA AGTTCTCAAA CACTATACCG 
ATGACTAAGG AACAGCAAGA AGCAGATGAA AAAGATTTGG ATGACGCGAT AGCGAATGCT 
AAATCAATAG AGAATGAGAA TTCCAACATT AGCGAACAGT TGGAATGTGA AGAGACGCGG 
CCCCGGAAAC CACTCGGAAA ACACGCTGGA AAGCCAGACT TGTCGGAAGT AGAATTGGAC 
TTAAAGAAGA GAAAATTGCA ACAGAATAAG AATAAATATA TTGCTAATGT TTTTGAAAAA 
CCATTAAAAG TAAATAAGAA TGAAATGGAA GTGAAACCAG AAAATCAAAC TACAACAGTT 
GAGAAGCAAT TAAATGTGGT TGAATTAAAA AATAGTAAAA ATCATGGAGC ACAAATTGGT 
TCTGAATGCA AAAAGGTACA ACGGAAGTTG ATTACAAAAA AACAGAAAGA ACTCCCTCAA 
TTGTTAGAGG GAAAGTCATT TGCAGGACAA AATAAATTGA AGCAGAAATC GAAACTGATT 
GGTCAATTGA AAAAAGACAA ATCCATGAAA AAGCACAAAA ATACAATTTG CTAA