LOC105221298 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105221298
Entrez Gene ID	105221298
Full Name	phosphatidylinositol glycan anchor biosynthesis, class O
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011198142.1	XP_011196444.1	GPI ethanolamine phosphate transferase 3
XM_011198142.2	XP_011196444.1	GPI ethanolamine phosphate transferase 3

Pathways from BioSystems
Gene Ontology

The following LOC105221298 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105221298 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa193521	XM_011198142.2 Latest version!	Zeugodacus cucurbitae GPI ethanolamine phosphate transferase 3 (LOC105221298), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OBa193521	XM_011198142.1	Bactrocera cucurbitae GPI ethanolamine phosphate transferase 3 (LOC105221298), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105221298 ( XM_011198142.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196444.1 Bactrocera cucurbitae GPI ethanolamine phosphate transferase 3 (LOC105221298), mRNA.

Price & Availability↑

CloneID	OBa193521
Clone ID Related Accession (Same CDS sequence)	XM_011198142.1 , XM_011198142.2
Accession Version	XM_011198142.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	GPI ethanolamine phosphate transferase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTATG CGTGGAGTTA TTTCTTCGTT CTAATATGGT TGGCTTATTT AGTTTCATCG 
GGGGTCCTGC TTTTCTCCCG AGGTTTTCTG CTTTCACGTG TATCCAAAAC GGATGTCAGC 
TCCTGTCAAC GCCTATCATT ATATGAAAGT GATGAATATT TTTTAAGTGA GGAAATTGTA 
CAAGAAATTT TCAAGGATGT AAACGCTACT TCCAATTTGT GTCTGCCTCA AAAGTCTAAA 
GTGATTATTT TGCTCATTGA CGCGCTAAAA TATGAATTCG GCCTTTATAA GGAAAATATT 
ACTAATCCAC TTCCTTACCA AAATAAGCTA ACAATAATGT ATGATTTAAT ACAAAGCTCT 
CCTGAAAATG CACACCTTAT GCAATTTAGA GCCGATCCAC CAACAACCAC TTTACAACGT 
CTTAAAGGAC TTACAACCGG TAGTTTACCC ACATTTGTTG ACATGGGTTC GAACTTCGCT 
TCTCCCGAAA TTAACGAGGA CAATATTATT GATCAGCTAG TCGGACATAA CCTTCCCATT 
GTGTTTTTGG GCGACAGCAC CTGGACAGAT CTGTATCCCA GACGATTTAA GCGCGCTTAT 
TCCTATCCTA GCTTCGACAT ATTCGATTTA GATACCGTCG ACAACAAAAT TGAACAATTT 
TTGCCAAAGG AACTGGGAAA GGATGACTGG CAGGTTCTTA TTGCACACTT TTTGGGAGTT 
GATCATTGTG GCCACAAACA CGGACCGTTG CATGAAGAGA TGGGCCGTAA ACTCAATGAA 
ATGAATGAGG TCATAAAATC AGTTATTGCA AAAATGGACA GTGATACAAC TTTGCTTGTG 
ATGGGTGATC ATGGCATGAC GGCATCTGGG GATCATGGCG GTGAAACTGA TGATGAAACT 
AATGCGTTGT TATTTGCCTA TACTAAAGGC AATAAATTCA TCGGAAGCGA ATTCGGTTCA 
GACAACACAA CTATGCAACA GATCGACCTC GTGCCAACCT TAGCCACTAT ATTAGGCGTC 
CCCATACCCT ACTCAAATTT GGGTTTAATC AACTTCAATA TCGTACCTGA TATTAAGGTA 
CCGTATATGA ACAAGTTCCA GACCCTCTTA CTTCATGTCT GGCAAAATGC GCAGCAGATG 
TACGAGTACT TCTTCAACTA CGCTTTGGAG AACAAGCGTA CCTTCAGCGT TGATGAATTG 
GAGAACATGC ATAACGAATA CATATTATTC ACACATCGCG TCAAAACGGT GTACAATGAA 
GCGGGCTTCA AATCATTTGT AGGCGATCTA AATGCGAATA TGCGACTAAT ACTGAACACA 
TGCCGTGAAA TTTGGGTGAA ATTCGACGCT GTGCAAATGT CCCAGGGGCT CTTGTTTACG 
GTGCTGCCAA TATTCTTCGC ATTTGTGGTA ATCAACAATT TAAACGCCCT AGAGCTCTAC 
CGCATATTCA CACCGAAGGC CGTGACATAT ACCTATCTCG TGAATATTGC GGCAGGTTTC 
TTTGGCTATC GCTATTTCAA AAACTTCTCC TTCAAATCGG AGGATCATGG CGTGATATTC 
TTTACCAACA TCATCAGCAC ATTTATCCTC ATTTTTCACG CGTTGCAAAA TTGGCGTACA 
ATCGCCAACA ATTTGTCGTC GGTGAAGCGT TTCGGCAATA TGCCAACTCG GATTATTTTC 
CTCATCGCCG TCAGTGTATT CTTCTCCAAC AGCTTTATCA TACAGGAACC GAAGATCCTA 
TCATACCTCT TGGTAGGATC CATTCTACTG CTCGCATATG ATATTCTTCA GAAATGTGTA 
CGACTTGAGC TGAATAAGCG CTTCAAATGG TCACAACTTT TACGCTCCAC CACCACGCAG 
TTGCTGCTGT ACAGCGTATT GGCCATCATT ATCGTTCGTT ATGCATATGC GCTTTTTCGT 
TGCAGAGAGG AACAGGGCAA TTGCGTCGAT TTCTCCAGTA ATGCTGATGG TTTCTCGATA 
AAACGTCCCG CAACCGCCAA AACCTACATT TTAGCAGTTG TCATCATAGT ACTGTACACA 
ACGCTCTCAC GTCTCTATCT CAGGCACAGC GGTAGCTTAA CCGGTGGCTC GCCCAACGTT 
TGGCTGGCAC GTTATGGGCC CACAATCGCA TCGATTTGCG CTGGCGGACA TTTGCTGCTC 
GCTCATAGTG CTGTTAAGAA TGTGAAACGC ACACACATCG ATGCCATGGC GCTGGTGATT 
TACGGCCTCA TGCTGGTGCA GATCGTTGTG CTATGCGTCT CGCCATTGTT GACCTTCTAC 
GTGTTGCCCA ACTTCAGTAC GCCGATTCAA ATTAGGCCGT GGGACAATGT CATACCACAG 
ATATTCAAGA AGATCAAGCG CATGTATGAT GACGCGGAGG CGCGTGTGAA TCAAATGGAT 
GCAATGGTCT ATGGGCTGAC CACTGTATAC TCCGCAGCCA CGATTGTGTT TGGACTGTTT 
TTGAGTATGG TGCTGATTGT CTTACTCGAA CCGCGTGCCA CGATCGGTAT GATCTCGTGC 
ATAGCCATCG CAGTGGTCAT CATCTTGCTT CATTCTACAT TACGTTTTCG AACGGCTGCG 
AATCTAGCAA ATTGCATACA GCCGACATTC ACCGGCATAG TCAGTTGGTT TCTACTGGCA 
CACTTTTGCT TCTTCGCCAC GTCCCATCAA ACTACGCTCT CGCAGATAGA TTGGCGAGCT 
GCTTTCGTGG GACGCACAAG TGGCCTGGGG AAATCGCATT TGATTTCCGG CGTCTTGGTT 
ATATTGAATA CATTCTGCGG ACAAGTCTTC TTTTTCTGTA TGTACAATCT GCTCAGTTTG 
TTAACATTCT CACTTTGCGC GCTTCATCCA AAACTGATAA AAAGCACAAC TGCTGCAAAC 
ACGAATGGCG GTCCAGCCGC ATCACCGCTC GAAGGAATTG GATTGGACAT GATGCGCGGC 
GAATTCTTGC TGTATGAAAA TGAAGGCACC TTCCTTGCCA GCTCTTTTAA GCTCGCAGTG 
CAGTTTTTAA TACTCCAAGG GATTAGGGTG TTCTGTGCGA TGCTAGCGTG CACCATTCAC 
TGTCGTCATC TGATGGTGTG GAAGATTTTC GCGCCGCGCT TCATTTACGA GGGCATCGGA 
ACGTTTGTCA GCCTGGCGGC TCTGATTGTT GGTTACTTCG TTATTCTGCG AATTAATCGC 
GCTTTAGAAC GTTTAATTTT CAAGGCGAAC AACTTGAAAT TAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196444.1
CDS	289..3516
Translation	MNYAWSYFFVLIWLAYLVSSGVLLFSRGFLLSRVSKTDVSSCQRLSLYESDEYFLSEEIVQEIFKDVNATSNLCLPQKSKVIILLIDALKYEFGLYKENITNPLPYQNKLTIMYDLIQSSPENAHLMQFRADPPTTTLQRLKGLTTGSLPTFVDMGSNFASPEINEDNIIDQLVGHNLPIVFLGDSTWTDLYPRRFKRAYSYPSFDIFDLDTVDNKIEQFLPKELGKDDWQVLIAHFLGVDHCGHKHGPLHEEMGRKLNEMNEVIKSVIAKMDSDTTLLVMGDHGMTASGDHGGETDDETNALLFAYTKGNKFIGSEFGSDNTTMQQIDLVPTLATILGVPIPYSNLGLINFNIVPDIKVPYMNKFQTLLLHVWQNAQQMYEYFFNYALENKRTFSVDELENMHNEYILFTHRVKTVYNEAGFKSFVGDLNANMRLILNTCREIWVKFDAVQMSQGLLFTVLPIFFAFVVINNLNALELYRIFTPKAVTYTYLVNIAAGFFGYRYFKNFSFKSEDHGVIFFTNIISTFILIFHALQNWRTIANNLSSVKRFGNMPTRIIFLIAVSVFFSNSFIIQEPKILSYLLVGSILLLAYDILQKCVRLELNKRFKWSQLLRSTTTQLLLYSVLAIIIVRYAYALFRCREEQGNCVDFSSNADGFSIKRPATAKTYILAVVIIVLYTTLSRLYLRHSGSLTGGSPNVWLARYGPTIASICAGGHLLLAHSAVKNVKRTHIDAMALVIYGLMLVQIVVLCVSPLLTFYVLPNFSTPIQIRPWDNVIPQIFKKIKRMYDDAEARVNQMDAMVYGLTTVYSAATIVFGLFLSMVLIVLLEPRATIGMISCIAIAVVIILLHSTLRFRTAANLANCIQPTFTGIVSWFLLAHFCFFATSHQTTLSQIDWRAAFVGRTSGLGKSHLISGVLVILNTFCGQVFFFCMYNLLSLLTFSLCALHPKLIKSTTAANTNGGPAASPLEGIGLDMMRGEFLLYENEGTFLASSFKLAVQFLILQGIRVFCAMLACTIHCRHLMVWKIFAPRFIYEGIGTFVSLAALIVGYFVILRINRALERLIFKANNLKLD

Target ORF information:

RefSeq Version	XM_011198142.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae GPI ethanolamine phosphate transferase 3 (LOC105221298), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011198142.1

ORF Insert Sequence:

ATGAATTATG CGTGGAGTTA TTTCTTCGTT CTAATATGGT TGGCTTATTT AGTTTCATCG 
GGGGTCCTGC TTTTCTCCCG AGGTTTTCTG CTTTCACGTG TATCCAAAAC GGATGTCAGC 
TCCTGTCAAC GCCTATCATT ATATGAAAGT GATGAATATT TTTTAAGTGA GGAAATTGTA 
CAAGAAATTT TCAAGGATGT AAACGCTACT TCCAATTTGT GTCTGCCTCA AAAGTCTAAA 
GTGATTATTT TGCTCATTGA CGCGCTAAAA TATGAATTCG GCCTTTATAA GGAAAATATT 
ACTAATCCAC TTCCTTACCA AAATAAGCTA ACAATAATGT ATGATTTAAT ACAAAGCTCT 
CCTGAAAATG CACACCTTAT GCAATTTAGA GCCGATCCAC CAACAACCAC TTTACAACGT 
CTTAAAGGAC TTACAACCGG TAGTTTACCC ACATTTGTTG ACATGGGTTC GAACTTCGCT 
TCTCCCGAAA TTAACGAGGA CAATATTATT GATCAGCTAG TCGGACATAA CCTTCCCATT 
GTGTTTTTGG GCGACAGCAC CTGGACAGAT CTGTATCCCA GACGATTTAA GCGCGCTTAT 
TCCTATCCTA GCTTCGACAT ATTCGATTTA GATACCGTCG ACAACAAAAT TGAACAATTT 
TTGCCAAAGG AACTGGGAAA GGATGACTGG CAGGTTCTTA TTGCACACTT TTTGGGAGTT 
GATCATTGTG GCCACAAACA CGGACCGTTG CATGAAGAGA TGGGCCGTAA ACTCAATGAA 
ATGAATGAGG TCATAAAATC AGTTATTGCA AAAATGGACA GTGATACAAC TTTGCTTGTG 
ATGGGTGATC ATGGCATGAC GGCATCTGGG GATCATGGCG GTGAAACTGA TGATGAAACT 
AATGCGTTGT TATTTGCCTA TACTAAAGGC AATAAATTCA TCGGAAGCGA ATTCGGTTCA 
GACAACACAA CTATGCAACA GATCGACCTC GTGCCAACCT TAGCCACTAT ATTAGGCGTC 
CCCATACCCT ACTCAAATTT GGGTTTAATC AACTTCAATA TCGTACCTGA TATTAAGGTA 
CCGTATATGA ACAAGTTCCA GACCCTCTTA CTTCATGTCT GGCAAAATGC GCAGCAGATG 
TACGAGTACT TCTTCAACTA CGCTTTGGAG AACAAGCGTA CCTTCAGCGT TGATGAATTG 
GAGAACATGC ATAACGAATA CATATTATTC ACACATCGCG TCAAAACGGT GTACAATGAA 
GCGGGCTTCA AATCATTTGT AGGCGATCTA AATGCGAATA TGCGACTAAT ACTGAACACA 
TGCCGTGAAA TTTGGGTGAA ATTCGACGCT GTGCAAATGT CCCAGGGGCT CTTGTTTACG 
GTGCTGCCAA TATTCTTCGC ATTTGTGGTA ATCAACAATT TAAACGCCCT AGAGCTCTAC 
CGCATATTCA CACCGAAGGC CGTGACATAT ACCTATCTCG TGAATATTGC GGCAGGTTTC 
TTTGGCTATC GCTATTTCAA AAACTTCTCC TTCAAATCGG AGGATCATGG CGTGATATTC 
TTTACCAACA TCATCAGCAC ATTTATCCTC ATTTTTCACG CGTTGCAAAA TTGGCGTACA 
ATCGCCAACA ATTTGTCGTC GGTGAAGCGT TTCGGCAATA TGCCAACTCG GATTATTTTC 
CTCATCGCCG TCAGTGTATT CTTCTCCAAC AGCTTTATCA TACAGGAACC GAAGATCCTA 
TCATACCTCT TGGTAGGATC CATTCTACTG CTCGCATATG ATATTCTTCA GAAATGTGTA 
CGACTTGAGC TGAATAAGCG CTTCAAATGG TCACAACTTT TACGCTCCAC CACCACGCAG 
TTGCTGCTGT ACAGCGTATT GGCCATCATT ATCGTTCGTT ATGCATATGC GCTTTTTCGT 
TGCAGAGAGG AACAGGGCAA TTGCGTCGAT TTCTCCAGTA ATGCTGATGG TTTCTCGATA 
AAACGTCCCG CAACCGCCAA AACCTACATT TTAGCAGTTG TCATCATAGT ACTGTACACA 
ACGCTCTCAC GTCTCTATCT CAGGCACAGC GGTAGCTTAA CCGGTGGCTC GCCCAACGTT 
TGGCTGGCAC GTTATGGGCC CACAATCGCA TCGATTTGCG CTGGCGGACA TTTGCTGCTC 
GCTCATAGTG CTGTTAAGAA TGTGAAACGC ACACACATCG ATGCCATGGC GCTGGTGATT 
TACGGCCTCA TGCTGGTGCA GATCGTTGTG CTATGCGTCT CGCCATTGTT GACCTTCTAC 
GTGTTGCCCA ACTTCAGTAC GCCGATTCAA ATTAGGCCGT GGGACAATGT CATACCACAG 
ATATTCAAGA AGATCAAGCG CATGTATGAT GACGCGGAGG CGCGTGTGAA TCAAATGGAT 
GCAATGGTCT ATGGGCTGAC CACTGTATAC TCCGCAGCCA CGATTGTGTT TGGACTGTTT 
TTGAGTATGG TGCTGATTGT CTTACTCGAA CCGCGTGCCA CGATCGGTAT GATCTCGTGC 
ATAGCCATCG CAGTGGTCAT CATCTTGCTT CATTCTACAT TACGTTTTCG AACGGCTGCG 
AATCTAGCAA ATTGCATACA GCCGACATTC ACCGGCATAG TCAGTTGGTT TCTACTGGCA 
CACTTTTGCT TCTTCGCCAC GTCCCATCAA ACTACGCTCT CGCAGATAGA TTGGCGAGCT 
GCTTTCGTGG GACGCACAAG TGGCCTGGGG AAATCGCATT TGATTTCCGG CGTCTTGGTT 
ATATTGAATA CATTCTGCGG ACAAGTCTTC TTTTTCTGTA TGTACAATCT GCTCAGTTTG 
TTAACATTCT CACTTTGCGC GCTTCATCCA AAACTGATAA AAAGCACAAC TGCTGCAAAC 
ACGAATGGCG GTCCAGCCGC ATCACCGCTC GAAGGAATTG GATTGGACAT GATGCGCGGC 
GAATTCTTGC TGTATGAAAA TGAAGGCACC TTCCTTGCCA GCTCTTTTAA GCTCGCAGTG 
CAGTTTTTAA TACTCCAAGG GATTAGGGTG TTCTGTGCGA TGCTAGCGTG CACCATTCAC 
TGTCGTCATC TGATGGTGTG GAAGATTTTC GCGCCGCGCT TCATTTACGA GGGCATCGGA 
ACGTTTGTCA GCCTGGCGGC TCTGATTGTT GGTTACTTCG TTATTCTGCG AATTAATCGC 
GCTTTAGAAC GTTTAATTTT CAAGGCGAAC AACTTGAAAT TAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221298 ( XM_011198142.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196444.1 Zeugodacus cucurbitae GPI ethanolamine phosphate transferase 3 (LOC105221298), mRNA.

Price & Availability↑

CloneID	OBa193521
Clone ID Related Accession (Same CDS sequence)	XM_011198142.1 , XM_011198142.2
Accession Version	XM_011198142.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	GPI ethanolamine phosphate transferase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011198142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTATG CGTGGAGTTA TTTCTTCGTT CTAATATGGT TGGCTTATTT AGTTTCATCG 
GGGGTCCTGC TTTTCTCCCG AGGTTTTCTG CTTTCACGTG TATCCAAAAC GGATGTCAGC 
TCCTGTCAAC GCCTATCATT ATATGAAAGT GATGAATATT TTTTAAGTGA GGAAATTGTA 
CAAGAAATTT TCAAGGATGT AAACGCTACT TCCAATTTGT GTCTGCCTCA AAAGTCTAAA 
GTGATTATTT TGCTCATTGA CGCGCTAAAA TATGAATTCG GCCTTTATAA GGAAAATATT 
ACTAATCCAC TTCCTTACCA AAATAAGCTA ACAATAATGT ATGATTTAAT ACAAAGCTCT 
CCTGAAAATG CACACCTTAT GCAATTTAGA GCCGATCCAC CAACAACCAC TTTACAACGT 
CTTAAAGGAC TTACAACCGG TAGTTTACCC ACATTTGTTG ACATGGGTTC GAACTTCGCT 
TCTCCCGAAA TTAACGAGGA CAATATTATT GATCAGCTAG TCGGACATAA CCTTCCCATT 
GTGTTTTTGG GCGACAGCAC CTGGACAGAT CTGTATCCCA GACGATTTAA GCGCGCTTAT 
TCCTATCCTA GCTTCGACAT ATTCGATTTA GATACCGTCG ACAACAAAAT TGAACAATTT 
TTGCCAAAGG AACTGGGAAA GGATGACTGG CAGGTTCTTA TTGCACACTT TTTGGGAGTT 
GATCATTGTG GCCACAAACA CGGACCGTTG CATGAAGAGA TGGGCCGTAA ACTCAATGAA 
ATGAATGAGG TCATAAAATC AGTTATTGCA AAAATGGACA GTGATACAAC TTTGCTTGTG 
ATGGGTGATC ATGGCATGAC GGCATCTGGG GATCATGGCG GTGAAACTGA TGATGAAACT 
AATGCGTTGT TATTTGCCTA TACTAAAGGC AATAAATTCA TCGGAAGCGA ATTCGGTTCA 
GACAACACAA CTATGCAACA GATCGACCTC GTGCCAACCT TAGCCACTAT ATTAGGCGTC 
CCCATACCCT ACTCAAATTT GGGTTTAATC AACTTCAATA TCGTACCTGA TATTAAGGTA 
CCGTATATGA ACAAGTTCCA GACCCTCTTA CTTCATGTCT GGCAAAATGC GCAGCAGATG 
TACGAGTACT TCTTCAACTA CGCTTTGGAG AACAAGCGTA CCTTCAGCGT TGATGAATTG 
GAGAACATGC ATAACGAATA CATATTATTC ACACATCGCG TCAAAACGGT GTACAATGAA 
GCGGGCTTCA AATCATTTGT AGGCGATCTA AATGCGAATA TGCGACTAAT ACTGAACACA 
TGCCGTGAAA TTTGGGTGAA ATTCGACGCT GTGCAAATGT CCCAGGGGCT CTTGTTTACG 
GTGCTGCCAA TATTCTTCGC ATTTGTGGTA ATCAACAATT TAAACGCCCT AGAGCTCTAC 
CGCATATTCA CACCGAAGGC CGTGACATAT ACCTATCTCG TGAATATTGC GGCAGGTTTC 
TTTGGCTATC GCTATTTCAA AAACTTCTCC TTCAAATCGG AGGATCATGG CGTGATATTC 
TTTACCAACA TCATCAGCAC ATTTATCCTC ATTTTTCACG CGTTGCAAAA TTGGCGTACA 
ATCGCCAACA ATTTGTCGTC GGTGAAGCGT TTCGGCAATA TGCCAACTCG GATTATTTTC 
CTCATCGCCG TCAGTGTATT CTTCTCCAAC AGCTTTATCA TACAGGAACC GAAGATCCTA 
TCATACCTCT TGGTAGGATC CATTCTACTG CTCGCATATG ATATTCTTCA GAAATGTGTA 
CGACTTGAGC TGAATAAGCG CTTCAAATGG TCACAACTTT TACGCTCCAC CACCACGCAG 
TTGCTGCTGT ACAGCGTATT GGCCATCATT ATCGTTCGTT ATGCATATGC GCTTTTTCGT 
TGCAGAGAGG AACAGGGCAA TTGCGTCGAT TTCTCCAGTA ATGCTGATGG TTTCTCGATA 
AAACGTCCCG CAACCGCCAA AACCTACATT TTAGCAGTTG TCATCATAGT ACTGTACACA 
ACGCTCTCAC GTCTCTATCT CAGGCACAGC GGTAGCTTAA CCGGTGGCTC GCCCAACGTT 
TGGCTGGCAC GTTATGGGCC CACAATCGCA TCGATTTGCG CTGGCGGACA TTTGCTGCTC 
GCTCATAGTG CTGTTAAGAA TGTGAAACGC ACACACATCG ATGCCATGGC GCTGGTGATT 
TACGGCCTCA TGCTGGTGCA GATCGTTGTG CTATGCGTCT CGCCATTGTT GACCTTCTAC 
GTGTTGCCCA ACTTCAGTAC GCCGATTCAA ATTAGGCCGT GGGACAATGT CATACCACAG 
ATATTCAAGA AGATCAAGCG CATGTATGAT GACGCGGAGG CGCGTGTGAA TCAAATGGAT 
GCAATGGTCT ATGGGCTGAC CACTGTATAC TCCGCAGCCA CGATTGTGTT TGGACTGTTT 
TTGAGTATGG TGCTGATTGT CTTACTCGAA CCGCGTGCCA CGATCGGTAT GATCTCGTGC 
ATAGCCATCG CAGTGGTCAT CATCTTGCTT CATTCTACAT TACGTTTTCG AACGGCTGCG 
AATCTAGCAA ATTGCATACA GCCGACATTC ACCGGCATAG TCAGTTGGTT TCTACTGGCA 
CACTTTTGCT TCTTCGCCAC GTCCCATCAA ACTACGCTCT CGCAGATAGA TTGGCGAGCT 
GCTTTCGTGG GACGCACAAG TGGCCTGGGG AAATCGCATT TGATTTCCGG CGTCTTGGTT 
ATATTGAATA CATTCTGCGG ACAAGTCTTC TTTTTCTGTA TGTACAATCT GCTCAGTTTG 
TTAACATTCT CACTTTGCGC GCTTCATCCA AAACTGATAA AAAGCACAAC TGCTGCAAAC 
ACGAATGGCG GTCCAGCCGC ATCACCGCTC GAAGGAATTG GATTGGACAT GATGCGCGGC 
GAATTCTTGC TGTATGAAAA TGAAGGCACC TTCCTTGCCA GCTCTTTTAA GCTCGCAGTG 
CAGTTTTTAA TACTCCAAGG GATTAGGGTG TTCTGTGCGA TGCTAGCGTG CACCATTCAC 
TGTCGTCATC TGATGGTGTG GAAGATTTTC GCGCCGCGCT TCATTTACGA GGGCATCGGA 
ACGTTTGTCA GCCTGGCGGC TCTGATTGTT GGTTACTTCG TTATTCTGCG AATTAATCGC 
GCTTTAGAAC GTTTAATTTT CAAGGCGAAC AACTTGAAAT TAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196444.1
CDS	288..3515
Translation	MNYAWSYFFVLIWLAYLVSSGVLLFSRGFLLSRVSKTDVSSCQRLSLYESDEYFLSEEIVQEIFKDVNATSNLCLPQKSKVIILLIDALKYEFGLYKENITNPLPYQNKLTIMYDLIQSSPENAHLMQFRADPPTTTLQRLKGLTTGSLPTFVDMGSNFASPEINEDNIIDQLVGHNLPIVFLGDSTWTDLYPRRFKRAYSYPSFDIFDLDTVDNKIEQFLPKELGKDDWQVLIAHFLGVDHCGHKHGPLHEEMGRKLNEMNEVIKSVIAKMDSDTTLLVMGDHGMTASGDHGGETDDETNALLFAYTKGNKFIGSEFGSDNTTMQQIDLVPTLATILGVPIPYSNLGLINFNIVPDIKVPYMNKFQTLLLHVWQNAQQMYEYFFNYALENKRTFSVDELENMHNEYILFTHRVKTVYNEAGFKSFVGDLNANMRLILNTCREIWVKFDAVQMSQGLLFTVLPIFFAFVVINNLNALELYRIFTPKAVTYTYLVNIAAGFFGYRYFKNFSFKSEDHGVIFFTNIISTFILIFHALQNWRTIANNLSSVKRFGNMPTRIIFLIAVSVFFSNSFIIQEPKILSYLLVGSILLLAYDILQKCVRLELNKRFKWSQLLRSTTTQLLLYSVLAIIIVRYAYALFRCREEQGNCVDFSSNADGFSIKRPATAKTYILAVVIIVLYTTLSRLYLRHSGSLTGGSPNVWLARYGPTIASICAGGHLLLAHSAVKNVKRTHIDAMALVIYGLMLVQIVVLCVSPLLTFYVLPNFSTPIQIRPWDNVIPQIFKKIKRMYDDAEARVNQMDAMVYGLTTVYSAATIVFGLFLSMVLIVLLEPRATIGMISCIAIAVVIILLHSTLRFRTAANLANCIQPTFTGIVSWFLLAHFCFFATSHQTTLSQIDWRAAFVGRTSGLGKSHLISGVLVILNTFCGQVFFFCMYNLLSLLTFSLCALHPKLIKSTTAANTNGGPAASPLEGIGLDMMRGEFLLYENEGTFLASSFKLAVQFLILQGIRVFCAMLACTIHCRHLMVWKIFAPRFIYEGIGTFVSLAALIVGYFVILRINRALERLIFKANNLKLD

Target ORF information:

RefSeq Version	XM_011198142.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae GPI ethanolamine phosphate transferase 3 (LOC105221298), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011198142.2

ORF Insert Sequence:

ATGAATTATG CGTGGAGTTA TTTCTTCGTT CTAATATGGT TGGCTTATTT AGTTTCATCG 
GGGGTCCTGC TTTTCTCCCG AGGTTTTCTG CTTTCACGTG TATCCAAAAC GGATGTCAGC 
TCCTGTCAAC GCCTATCATT ATATGAAAGT GATGAATATT TTTTAAGTGA GGAAATTGTA 
CAAGAAATTT TCAAGGATGT AAACGCTACT TCCAATTTGT GTCTGCCTCA AAAGTCTAAA 
GTGATTATTT TGCTCATTGA CGCGCTAAAA TATGAATTCG GCCTTTATAA GGAAAATATT 
ACTAATCCAC TTCCTTACCA AAATAAGCTA ACAATAATGT ATGATTTAAT ACAAAGCTCT 
CCTGAAAATG CACACCTTAT GCAATTTAGA GCCGATCCAC CAACAACCAC TTTACAACGT 
CTTAAAGGAC TTACAACCGG TAGTTTACCC ACATTTGTTG ACATGGGTTC GAACTTCGCT 
TCTCCCGAAA TTAACGAGGA CAATATTATT GATCAGCTAG TCGGACATAA CCTTCCCATT 
GTGTTTTTGG GCGACAGCAC CTGGACAGAT CTGTATCCCA GACGATTTAA GCGCGCTTAT 
TCCTATCCTA GCTTCGACAT ATTCGATTTA GATACCGTCG ACAACAAAAT TGAACAATTT 
TTGCCAAAGG AACTGGGAAA GGATGACTGG CAGGTTCTTA TTGCACACTT TTTGGGAGTT 
GATCATTGTG GCCACAAACA CGGACCGTTG CATGAAGAGA TGGGCCGTAA ACTCAATGAA 
ATGAATGAGG TCATAAAATC AGTTATTGCA AAAATGGACA GTGATACAAC TTTGCTTGTG 
ATGGGTGATC ATGGCATGAC GGCATCTGGG GATCATGGCG GTGAAACTGA TGATGAAACT 
AATGCGTTGT TATTTGCCTA TACTAAAGGC AATAAATTCA TCGGAAGCGA ATTCGGTTCA 
GACAACACAA CTATGCAACA GATCGACCTC GTGCCAACCT TAGCCACTAT ATTAGGCGTC 
CCCATACCCT ACTCAAATTT GGGTTTAATC AACTTCAATA TCGTACCTGA TATTAAGGTA 
CCGTATATGA ACAAGTTCCA GACCCTCTTA CTTCATGTCT GGCAAAATGC GCAGCAGATG 
TACGAGTACT TCTTCAACTA CGCTTTGGAG AACAAGCGTA CCTTCAGCGT TGATGAATTG 
GAGAACATGC ATAACGAATA CATATTATTC ACACATCGCG TCAAAACGGT GTACAATGAA 
GCGGGCTTCA AATCATTTGT AGGCGATCTA AATGCGAATA TGCGACTAAT ACTGAACACA 
TGCCGTGAAA TTTGGGTGAA ATTCGACGCT GTGCAAATGT CCCAGGGGCT CTTGTTTACG 
GTGCTGCCAA TATTCTTCGC ATTTGTGGTA ATCAACAATT TAAACGCCCT AGAGCTCTAC 
CGCATATTCA CACCGAAGGC CGTGACATAT ACCTATCTCG TGAATATTGC GGCAGGTTTC 
TTTGGCTATC GCTATTTCAA AAACTTCTCC TTCAAATCGG AGGATCATGG CGTGATATTC 
TTTACCAACA TCATCAGCAC ATTTATCCTC ATTTTTCACG CGTTGCAAAA TTGGCGTACA 
ATCGCCAACA ATTTGTCGTC GGTGAAGCGT TTCGGCAATA TGCCAACTCG GATTATTTTC 
CTCATCGCCG TCAGTGTATT CTTCTCCAAC AGCTTTATCA TACAGGAACC GAAGATCCTA 
TCATACCTCT TGGTAGGATC CATTCTACTG CTCGCATATG ATATTCTTCA GAAATGTGTA 
CGACTTGAGC TGAATAAGCG CTTCAAATGG TCACAACTTT TACGCTCCAC CACCACGCAG 
TTGCTGCTGT ACAGCGTATT GGCCATCATT ATCGTTCGTT ATGCATATGC GCTTTTTCGT 
TGCAGAGAGG AACAGGGCAA TTGCGTCGAT TTCTCCAGTA ATGCTGATGG TTTCTCGATA 
AAACGTCCCG CAACCGCCAA AACCTACATT TTAGCAGTTG TCATCATAGT ACTGTACACA 
ACGCTCTCAC GTCTCTATCT CAGGCACAGC GGTAGCTTAA CCGGTGGCTC GCCCAACGTT 
TGGCTGGCAC GTTATGGGCC CACAATCGCA TCGATTTGCG CTGGCGGACA TTTGCTGCTC 
GCTCATAGTG CTGTTAAGAA TGTGAAACGC ACACACATCG ATGCCATGGC GCTGGTGATT 
TACGGCCTCA TGCTGGTGCA GATCGTTGTG CTATGCGTCT CGCCATTGTT GACCTTCTAC 
GTGTTGCCCA ACTTCAGTAC GCCGATTCAA ATTAGGCCGT GGGACAATGT CATACCACAG 
ATATTCAAGA AGATCAAGCG CATGTATGAT GACGCGGAGG CGCGTGTGAA TCAAATGGAT 
GCAATGGTCT ATGGGCTGAC CACTGTATAC TCCGCAGCCA CGATTGTGTT TGGACTGTTT 
TTGAGTATGG TGCTGATTGT CTTACTCGAA CCGCGTGCCA CGATCGGTAT GATCTCGTGC 
ATAGCCATCG CAGTGGTCAT CATCTTGCTT CATTCTACAT TACGTTTTCG AACGGCTGCG 
AATCTAGCAA ATTGCATACA GCCGACATTC ACCGGCATAG TCAGTTGGTT TCTACTGGCA 
CACTTTTGCT TCTTCGCCAC GTCCCATCAA ACTACGCTCT CGCAGATAGA TTGGCGAGCT 
GCTTTCGTGG GACGCACAAG TGGCCTGGGG AAATCGCATT TGATTTCCGG CGTCTTGGTT 
ATATTGAATA CATTCTGCGG ACAAGTCTTC TTTTTCTGTA TGTACAATCT GCTCAGTTTG 
TTAACATTCT CACTTTGCGC GCTTCATCCA AAACTGATAA AAAGCACAAC TGCTGCAAAC 
ACGAATGGCG GTCCAGCCGC ATCACCGCTC GAAGGAATTG GATTGGACAT GATGCGCGGC 
GAATTCTTGC TGTATGAAAA TGAAGGCACC TTCCTTGCCA GCTCTTTTAA GCTCGCAGTG 
CAGTTTTTAA TACTCCAAGG GATTAGGGTG TTCTGTGCGA TGCTAGCGTG CACCATTCAC 
TGTCGTCATC TGATGGTGTG GAAGATTTTC GCGCCGCGCT TCATTTACGA GGGCATCGGA 
ACGTTTGTCA GCCTGGCGGC TCTGATTGTT GGTTACTTCG TTATTCTGCG AATTAATCGC 
GCTTTAGAAC GTTTAATTTT CAAGGCGAAC AACTTGAAAT TAGATTAA