LOC105218485 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105218485
Entrez Gene ID	105218485
Full Name	dual specificity mitogen-activated protein kinase kinase hemipterous-like
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011194101.1	XP_011192403.1	dual specificity mitogen-activated protein kinase kinase hemipterous-like
XM_011194101.2	XP_011192403.1	dual specificity mitogen-activated protein kinase kinase hemipterous-like

Pathways from BioSystems
Gene Ontology

The following LOC105218485 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105218485 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa205643	XM_011194101.2 Latest version!	Zeugodacus cucurbitae dual specificity mitogen-activated protein kinase kinase hemipterous-like (LOC105218485), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OBa205643	XM_011194101.1	Bactrocera cucurbitae dual specificity mitogen-activated protein kinase kinase hemipterous-like (LOC105218485), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105218485 ( XM_011194101.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011192403.1 Bactrocera cucurbitae dual specificity mitogen-activated protein kinase kinase hemipterous-like (LOC105218485), mRNA.

Price & Availability↑

CloneID	OBa205643
Clone ID Related Accession (Same CDS sequence)	XM_011194101.1 , XM_011194101.2
Accession Version	XM_011194101.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	dual specificity mitogen-activated protein kinase kinase hemipterous-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863865.1) and transcript sequence (GBXI01012708.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 235 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCACGACG CACCGGAGTA TAAACACAGC AGCAGTAGTA GCAGTCGTGA GCAACACCAA 
CAGCTAGGAA GAGTGCCAGT CCCCAACGCA GCAGGCACTG GAGCAAGGAT AACGCCAACC 
ACAGCTACGC ATATATCATC ATCATCAGCC GTTAGTAAAA CAAGCAATTC CTCAATGGAG 
TCCATTGGAC ATAGAATACA CAGACTAGAG AAACGTCTCG AGGAGGAGAA CAATTCACAC 
AATCACTTGA TGTTTGCTAC AGACCCTCCA AGTGGCTCTG CAACGCGTGT TGGCGCCAGA 
GATGTACACA TGTCGCTACC AATCGGCAGC AAGTCAAAAC GCCCCATCTT GGATTTGGTT 
ATGACAAATA CAAATCGTAA TGGTCCACAA ACAGAAATGC ACAAGAAACT TAAAAGAATA 
CGACAACAAT CCGGTATACT AACCATAAAT GGCCAAAAAT ATCCCAGTAG AATGTCTGAT 
TTGGAGCACT TGAGTGATTT GGGTAATGGC ACTAGTGGGA ATGTGGTAAA AATGCGACAC 
AAACCAACGG GCACGATTTT AGCCGTCAAA CAAATGCGTA GTACTGGCAA CGATGAAGAA 
AATAATCGTA TACTCATGGA TCTCGATGTT ATACTGAATT CACATGACTG CCCATATATA 
GTGCATTGTT TAGGTTATTT TGTAAATATA CCGGATGTGT GGATATGCAT GGAACTGATG 
TCAATGTGTT TCGATAAGCT GCTAAGGCGT TCAAAACAAC CGGTGCCAGA GAATATACTT 
GGGAAGGTTA CTGTAGCGAC GGTGCACGCT CTTTCATATT TAAAAAAGAA TCACAATGTC 
ATACATCGAG ATGTGAAACC TTCAAATATC CTCATCGACG AACAGGGCAA TATCAAATTG 
TGCGATTTCG GCATTAGCGG GCGACTTGTG GATTCAAAAG CGAATACACG TTCAGCCGGG 
TGCGCCGCTT ACATGGCGCC TGAACGCATC GATCCGAAAA AGCCGAAATA TGATATACGC 
GCTGATGTTT GGTCATTGGG CATAACACTA GTGGAACTTG CAACCGGCCG TTCACCGTAC 
GAAGGCTGCA ACATAGACTT TGAGGTGCTG ACAAAAATTT TGGATCACGA ACCACCCAGT 
TTGCCATATG GTGATGGTTT TGATTTCAGT CAAGAATTTC GTGATTTTGT CGATAAATGT 
CTGACGAAAA ATGTACATCA GCGCCCCAGA TACCAGGAGC TCTTGCAGCA ACCATTTTTT 
CGTTATTACG ACGCGGCTGA TGTGAATGTG ATTCAATGGT TTAAGACTGT TGTCGAATCT 
GCCGACATAG AGATGCATAG AACTCACATG CCAATTACTA CAACAACCAC AGCTACACTC 
AGAGGAGATG TAACAGCAGT AACAAATAAT AAACATAACA AAATTGCAAC TGCAACAATA 
ATGCCAACAG CAACAACAAT AATAGCAACA ACAACGACAA TAAATCCAAA AATGCCAGCA 
GCAAGCAGCA CAAATGCTAC CCACACACAC ATACCCAGCT ATCAGAATTC CTATCACAGT 
GCCACACACA CAACCAACGC GTCACAAACA CAATCCACAC AGCCACAAAT GTTTGCGTCA 
CGCTACAAAA AAGACGATTA TCTGCCACAA TATCAACCAC AATATGCACA CGCGCAAACG 
CAGTTGTCAC ATCATCATCA TCAACCATCA CAACTACCTC AGCCGATTGC AAGCTATTAC 
AACAACAGCA ACGCCAACAA TCATTTACAG GGCGAACGTG GTGGAGGCGG TAGCATCAGC 
GGCAGCACCG GTGTAGGTCT GAGTAGTGGT GTCGGTGGCG GTGTCGGTGT CAGTGGTGGC 
GGCAATGGTT ATGGCAGCGG TGGTTCGGGC AGCAATAGCG CGAATAGCAG CAGTCCACAA 
TCGCCGCCCA GCAGCAGCTA CAAGGATGCA ACGCACAAGG ACGACACCAC GCATATTGTC 
AGCGAGATGA GCAAATTGTA TCGCAAATCA CCGTTTCTGC AGCGCAAATA TAGCAATGGC 
TCGGGTTTGA AGTACACCAG CGCGGTGGGC GGTGCCGACA GTCCCAAAAA GGAATCCATG 
TTCAGCAGTA TAGGTCAATC GATCATACGC AATCTCACGA CCTCGCCCTT TAGCCAAAAG 
AAACATCATA CACCACAACC ACATCAGGTG GATCCACTTT GGCGCATGCC GAATGTGCAG 
GAGATGGGTG GCAATGCGTT CGATGTGTAT GATAGTCCCG CCATACCGAA GACGGTGGCG 
TTGGGTTCGC CAGCGATGAC GCGCAAGCGT TTTCAGGGTG GCGCCGCATC ACCAACACTG 
CCCATAGGCA CGGCGGTGGA GCCAGTTTTG AAGGAGCAGC AGCCTTCGCT TATACCAGTT 
AATCGCGGTG AGCTGAATAC GCAGCAGCGC GTGCCCGGCA ACCATAGTCC AATAGTGCTG 
CAACGTTTCT ACCATCAGCA GAATCAGCTG CGTGAGAAGG AGTTGGAAAA GCGTAAGGAG 
CATCTGCAAC AACAAAAGCA CTCCACAAAT CCCTTCCTCG CTGGCAATTA TCTCTCCACA 
ACGGCTGCCG TCACAGCGTC CACTACGCCA TTGCATTACA GCAACACCAA CAACAGCGCC 
GGCGGCGTTG GCAATCAACA GCACTATCAA CTGCCCCAGC CGCAGCAGCA ACGCTATCCG 
CAACAGCAGC CAACGCATCA CATGCCGCTA ACCAGCGCTG CCTCCTCATC ACATTCAACT 
TCTAGTCAAT CATCCACACA ATCCTCGTCC TCACAAATAG CCCTAAGCGA TTTGCACGAA 
CATCAACAAG CGCGTTCGCC ACCCACTTGC GCACCACCGC CACCGCCGCA AACATCTGCC 
GCGGCAATCA GCACTGCCTT TGGCACGAGT CCCAGTCAGC TGCAATACCA ACCGCTGCCG 
CAGCATTATT TACCGTTGAA TGAGCATGCA GTACGTAGTT CGCGTTCGCC GCCTACCTCG 
CCGGAGCAGC TGTCTTCCGA TGGCGGTGTG AGCGGTAGTG GCGGTGGTGG TGGCAGCAGC 
GGCATGGCGA GCAAGTTGAG CAAATTGTAT GCGCGTCGGC AGTGGCAAAC GACCACGGCG 
TCAACGATAC CGACGCATAC AGGCGTTGAG GGTGTGTGTG GCAGTGGCGG AGGGATCGTT 
GGAGGCGGTG TTGGGTTGGC GGGCAAGGAA TACAGGGACG AACATGGCTG GTTTAACACA 
CTTGCCGGCG CCATGAAGCG TCAATTTGCC TCATATGTTA AATTACAATT GCATTCTGCA 
GGCTCGCCTT CGACCAGTGG CGCAGCCAAT CAACATGACG GCGTCGGCTA TGAGAGTGCG 
CTGCCACCGA GCGCACAACC TGGCAAACCG ACACTCGCCT ATTATCGTAC GCTCTCCGCG 
GGTAGCAGCA GTAATACGAG CAACAGCACT TCGCCGACTG AGGCGCAGCC TAAACCTGGA 
CAAACGGCGG GTAGTGACTT CTACGATGGC ACCAGCGGCT TTCTGCGTCG CTATGCCGCG 
GCAGGCGCGA GCGGCATGGT CAGCGGTTCT TCGAATGCGC ATCTGCTGAC CGGTTTGGAT 
CGGCGACACC GCAGTCCAGA TCCACCGCCG CGCTACAATC GCGGCCAATC GCCACTGCTG 
TTGCGCAAGC AGTTGCTGGA GCTGAGCGGC CAGCCGCCAG GCGGTTCACC GCTGCTCAAT 
AGACGCTATG TGAACGCCTC ACCACCATTG CCACCACCGC GTCGCGGCTC GGAGAGTGTG 
CCCGGCTCAC CGCAACATTT TCGCACGCGC ATTCATTACA CACCCGAACC ACAGCGGCGC 
ATATATCGCA CGATCGACCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011192403.1
CDS	440..4303
Translation	MHDAPEYKHSSSSSSREQHQQLGRVPVPNAAGTGARITPTTATHISSSSAVSKTSNSSMESIGHRIHRLEKRLEEENNSHNHLMFATDPPSGSATRVGARDVHMSLPIGSKSKRPILDLVMTNTNRNGPQTEMHKKLKRIRQQSGILTINGQKYPSRMSDLEHLSDLGNGTSGNVVKMRHKPTGTILAVKQMRSTGNDEENNRILMDLDVILNSHDCPYIVHCLGYFVNIPDVWICMELMSMCFDKLLRRSKQPVPENILGKVTVATVHALSYLKKNHNVIHRDVKPSNILIDEQGNIKLCDFGISGRLVDSKANTRSAGCAAYMAPERIDPKKPKYDIRADVWSLGITLVELATGRSPYEGCNIDFEVLTKILDHEPPSLPYGDGFDFSQEFRDFVDKCLTKNVHQRPRYQELLQQPFFRYYDAADVNVIQWFKTVVESADIEMHRTHMPITTTTTATLRGDVTAVTNNKHNKIATATIMPTATTIIATTTTINPKMPAASSTNATHTHIPSYQNSYHSATHTTNASQTQSTQPQMFASRYKKDDYLPQYQPQYAHAQTQLSHHHHQPSQLPQPIASYYNNSNANNHLQGERGGGGSISGSTGVGLSSGVGGGVGVSGGGNGYGSGGSGSNSANSSSPQSPPSSSYKDATHKDDTTHIVSEMSKLYRKSPFLQRKYSNGSGLKYTSAVGGADSPKKESMFSSIGQSIIRNLTTSPFSQKKHHTPQPHQVDPLWRMPNVQEMGGNAFDVYDSPAIPKTVALGSPAMTRKRFQGGAASPTLPIGTAVEPVLKEQQPSLIPVNRGELNTQQRVPGNHSPIVLQRFYHQQNQLREKELEKRKEHLQQQKHSTNPFLAGNYLSTTAAVTASTTPLHYSNTNNSAGGVGNQQHYQLPQPQQQRYPQQQPTHHMPLTSAASSSHSTSSQSSTQSSSSQIALSDLHEHQQARSPPTCAPPPPPQTSAAAISTAFGTSPSQLQYQPLPQHYLPLNEHAVRSSRSPPTSPEQLSSDGGVSGSGGGGGSSGMASKLSKLYARRQWQTTTASTIPTHTGVEGVCGSGGGIVGGGVGLAGKEYRDEHGWFNTLAGAMKRQFASYVKLQLHSAGSPSTSGAANQHDGVGYESALPPSAQPGKPTLAYYRTLSAGSSSNTSNSTSPTEAQPKPGQTAGSDFYDGTSGFLRRYAAAGASGMVSGSSNAHLLTGLDRRHRSPDPPPRYNRGQSPLLLRKQLLELSGQPPGGSPLLNRRYVNASPPLPPPRRGSESVPGSPQHFRTRIHYTPEPQRRIYRTIDQ

Target ORF information:

RefSeq Version	XM_011194101.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae dual specificity mitogen-activated protein kinase kinase hemipterous-like (LOC105218485), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011194101.1

ORF Insert Sequence:

ATGCACGACG CACCGGAGTA TAAACACAGC AGCAGTAGTA GCAGTCGTGA GCAACACCAA 
CAGCTAGGAA GAGTGCCAGT CCCCAACGCA GCAGGCACTG GAGCAAGGAT AACGCCAACC 
ACAGCTACGC ATATATCATC ATCATCAGCC GTTAGTAAAA CAAGCAATTC CTCAATGGAG 
TCCATTGGAC ATAGAATACA CAGACTAGAG AAACGTCTCG AGGAGGAGAA CAATTCACAC 
AATCACTTGA TGTTTGCTAC AGACCCTCCA AGTGGCTCTG CAACGCGTGT TGGCGCCAGA 
GATGTACACA TGTCGCTACC AATCGGCAGC AAGTCAAAAC GCCCCATCTT GGATTTGGTT 
ATGACAAATA CAAATCGTAA TGGTCCACAA ACAGAAATGC ACAAGAAACT TAAAAGAATA 
CGACAACAAT CCGGTATACT AACCATAAAT GGCCAAAAAT ATCCCAGTAG AATGTCTGAT 
TTGGAGCACT TGAGTGATTT GGGTAATGGC ACTAGTGGGA ATGTGGTAAA AATGCGACAC 
AAACCAACGG GCACGATTTT AGCCGTCAAA CAAATGCGTA GTACTGGCAA CGATGAAGAA 
AATAATCGTA TACTCATGGA TCTCGATGTT ATACTGAATT CACATGACTG CCCATATATA 
GTGCATTGTT TAGGTTATTT TGTAAATATA CCGGATGTGT GGATATGCAT GGAACTGATG 
TCAATGTGTT TCGATAAGCT GCTAAGGCGT TCAAAACAAC CGGTGCCAGA GAATATACTT 
GGGAAGGTTA CTGTAGCGAC GGTGCACGCT CTTTCATATT TAAAAAAGAA TCACAATGTC 
ATACATCGAG ATGTGAAACC TTCAAATATC CTCATCGACG AACAGGGCAA TATCAAATTG 
TGCGATTTCG GCATTAGCGG GCGACTTGTG GATTCAAAAG CGAATACACG TTCAGCCGGG 
TGCGCCGCTT ACATGGCGCC TGAACGCATC GATCCGAAAA AGCCGAAATA TGATATACGC 
GCTGATGTTT GGTCATTGGG CATAACACTA GTGGAACTTG CAACCGGCCG TTCACCGTAC 
GAAGGCTGCA ACATAGACTT TGAGGTGCTG ACAAAAATTT TGGATCACGA ACCACCCAGT 
TTGCCATATG GTGATGGTTT TGATTTCAGT CAAGAATTTC GTGATTTTGT CGATAAATGT 
CTGACGAAAA ATGTACATCA GCGCCCCAGA TACCAGGAGC TCTTGCAGCA ACCATTTTTT 
CGTTATTACG ACGCGGCTGA TGTGAATGTG ATTCAATGGT TTAAGACTGT TGTCGAATCT 
GCCGACATAG AGATGCATAG AACTCACATG CCAATTACTA CAACAACCAC AGCTACACTC 
AGAGGAGATG TAACAGCAGT AACAAATAAT AAACATAACA AAATTGCAAC TGCAACAATA 
ATGCCAACAG CAACAACAAT AATAGCAACA ACAACGACAA TAAATCCAAA AATGCCAGCA 
GCAAGCAGCA CAAATGCTAC CCACACACAC ATACCCAGCT ATCAGAATTC CTATCACAGT 
GCCACACACA CAACCAACGC GTCACAAACA CAATCCACAC AGCCACAAAT GTTTGCGTCA 
CGCTACAAAA AAGACGATTA TCTGCCACAA TATCAACCAC AATATGCACA CGCGCAAACG 
CAGTTGTCAC ATCATCATCA TCAACCATCA CAACTACCTC AGCCGATTGC AAGCTATTAC 
AACAACAGCA ACGCCAACAA TCATTTACAG GGCGAACGTG GTGGAGGCGG TAGCATCAGC 
GGCAGCACCG GTGTAGGTCT GAGTAGTGGT GTCGGTGGCG GTGTCGGTGT CAGTGGTGGC 
GGCAATGGTT ATGGCAGCGG TGGTTCGGGC AGCAATAGCG CGAATAGCAG CAGTCCACAA 
TCGCCGCCCA GCAGCAGCTA CAAGGATGCA ACGCACAAGG ACGACACCAC GCATATTGTC 
AGCGAGATGA GCAAATTGTA TCGCAAATCA CCGTTTCTGC AGCGCAAATA TAGCAATGGC 
TCGGGTTTGA AGTACACCAG CGCGGTGGGC GGTGCCGACA GTCCCAAAAA GGAATCCATG 
TTCAGCAGTA TAGGTCAATC GATCATACGC AATCTCACGA CCTCGCCCTT TAGCCAAAAG 
AAACATCATA CACCACAACC ACATCAGGTG GATCCACTTT GGCGCATGCC GAATGTGCAG 
GAGATGGGTG GCAATGCGTT CGATGTGTAT GATAGTCCCG CCATACCGAA GACGGTGGCG 
TTGGGTTCGC CAGCGATGAC GCGCAAGCGT TTTCAGGGTG GCGCCGCATC ACCAACACTG 
CCCATAGGCA CGGCGGTGGA GCCAGTTTTG AAGGAGCAGC AGCCTTCGCT TATACCAGTT 
AATCGCGGTG AGCTGAATAC GCAGCAGCGC GTGCCCGGCA ACCATAGTCC AATAGTGCTG 
CAACGTTTCT ACCATCAGCA GAATCAGCTG CGTGAGAAGG AGTTGGAAAA GCGTAAGGAG 
CATCTGCAAC AACAAAAGCA CTCCACAAAT CCCTTCCTCG CTGGCAATTA TCTCTCCACA 
ACGGCTGCCG TCACAGCGTC CACTACGCCA TTGCATTACA GCAACACCAA CAACAGCGCC 
GGCGGCGTTG GCAATCAACA GCACTATCAA CTGCCCCAGC CGCAGCAGCA ACGCTATCCG 
CAACAGCAGC CAACGCATCA CATGCCGCTA ACCAGCGCTG CCTCCTCATC ACATTCAACT 
TCTAGTCAAT CATCCACACA ATCCTCGTCC TCACAAATAG CCCTAAGCGA TTTGCACGAA 
CATCAACAAG CGCGTTCGCC ACCCACTTGC GCACCACCGC CACCGCCGCA AACATCTGCC 
GCGGCAATCA GCACTGCCTT TGGCACGAGT CCCAGTCAGC TGCAATACCA ACCGCTGCCG 
CAGCATTATT TACCGTTGAA TGAGCATGCA GTACGTAGTT CGCGTTCGCC GCCTACCTCG 
CCGGAGCAGC TGTCTTCCGA TGGCGGTGTG AGCGGTAGTG GCGGTGGTGG TGGCAGCAGC 
GGCATGGCGA GCAAGTTGAG CAAATTGTAT GCGCGTCGGC AGTGGCAAAC GACCACGGCG 
TCAACGATAC CGACGCATAC AGGCGTTGAG GGTGTGTGTG GCAGTGGCGG AGGGATCGTT 
GGAGGCGGTG TTGGGTTGGC GGGCAAGGAA TACAGGGACG AACATGGCTG GTTTAACACA 
CTTGCCGGCG CCATGAAGCG TCAATTTGCC TCATATGTTA AATTACAATT GCATTCTGCA 
GGCTCGCCTT CGACCAGTGG CGCAGCCAAT CAACATGACG GCGTCGGCTA TGAGAGTGCG 
CTGCCACCGA GCGCACAACC TGGCAAACCG ACACTCGCCT ATTATCGTAC GCTCTCCGCG 
GGTAGCAGCA GTAATACGAG CAACAGCACT TCGCCGACTG AGGCGCAGCC TAAACCTGGA 
CAAACGGCGG GTAGTGACTT CTACGATGGC ACCAGCGGCT TTCTGCGTCG CTATGCCGCG 
GCAGGCGCGA GCGGCATGGT CAGCGGTTCT TCGAATGCGC ATCTGCTGAC CGGTTTGGAT 
CGGCGACACC GCAGTCCAGA TCCACCGCCG CGCTACAATC GCGGCCAATC GCCACTGCTG 
TTGCGCAAGC AGTTGCTGGA GCTGAGCGGC CAGCCGCCAG GCGGTTCACC GCTGCTCAAT 
AGACGCTATG TGAACGCCTC ACCACCATTG CCACCACCGC GTCGCGGCTC GGAGAGTGTG 
CCCGGCTCAC CGCAACATTT TCGCACGCGC ATTCATTACA CACCCGAACC ACAGCGGCGC 
ATATATCGCA CGATCGACCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105218485 ( XM_011194101.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011192403.1 Zeugodacus cucurbitae dual specificity mitogen-activated protein kinase kinase hemipterous-like (LOC105218485), mRNA.

Price & Availability↑

CloneID	OBa205643
Clone ID Related Accession (Same CDS sequence)	XM_011194101.1 , XM_011194101.2
Accession Version	XM_011194101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	dual specificity mitogen-activated protein kinase kinase hemipterous-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863865.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011194101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACG CACCGGAGTA TAAACACAGC AGCAGTAGTA GCAGTCGTGA GCAACACCAA 
CAGCTAGGAA GAGTGCCAGT CCCCAACGCA GCAGGCACTG GAGCAAGGAT AACGCCAACC 
ACAGCTACGC ATATATCATC ATCATCAGCC GTTAGTAAAA CAAGCAATTC CTCAATGGAG 
TCCATTGGAC ATAGAATACA CAGACTAGAG AAACGTCTCG AGGAGGAGAA CAATTCACAC 
AATCACTTGA TGTTTGCTAC AGACCCTCCA AGTGGCTCTG CAACGCGTGT TGGCGCCAGA 
GATGTACACA TGTCGCTACC AATCGGCAGC AAGTCAAAAC GCCCCATCTT GGATTTGGTT 
ATGACAAATA CAAATCGTAA TGGTCCACAA ACAGAAATGC ACAAGAAACT TAAAAGAATA 
CGACAACAAT CCGGTATACT AACCATAAAT GGCCAAAAAT ATCCCAGTAG AATGTCTGAT 
TTGGAGCACT TGAGTGATTT GGGTAATGGC ACTAGTGGGA ATGTGGTAAA AATGCGACAC 
AAACCAACGG GCACGATTTT AGCCGTCAAA CAAATGCGTA GTACTGGCAA CGATGAAGAA 
AATAATCGTA TACTCATGGA TCTCGATGTT ATACTGAATT CACATGACTG CCCATATATA 
GTGCATTGTT TAGGTTATTT TGTAAATATA CCGGATGTGT GGATATGCAT GGAACTGATG 
TCAATGTGTT TCGATAAGCT GCTAAGGCGT TCAAAACAAC CGGTGCCAGA GAATATACTT 
GGGAAGGTTA CTGTAGCGAC GGTGCACGCT CTTTCATATT TAAAAAAGAA TCACAATGTC 
ATACATCGAG ATGTGAAACC TTCAAATATC CTCATCGACG AACAGGGCAA TATCAAATTG 
TGCGATTTCG GCATTAGCGG GCGACTTGTG GATTCAAAAG CGAATACACG TTCAGCCGGG 
TGCGCCGCTT ACATGGCGCC TGAACGCATC GATCCGAAAA AGCCGAAATA TGATATACGC 
GCTGATGTTT GGTCATTGGG CATAACACTA GTGGAACTTG CAACCGGCCG TTCACCGTAC 
GAAGGCTGCA ACATAGACTT TGAGGTGCTG ACAAAAATTT TGGATCACGA ACCACCCAGT 
TTGCCATATG GTGATGGTTT TGATTTCAGT CAAGAATTTC GTGATTTTGT CGATAAATGT 
CTGACGAAAA ATGTACATCA GCGCCCCAGA TACCAGGAGC TCTTGCAGCA ACCATTTTTT 
CGTTATTACG ACGCGGCTGA TGTGAATGTG ATTCAATGGT TTAAGACTGT TGTCGAATCT 
GCCGACATAG AGATGCATAG AACTCACATG CCAATTACTA CAACAACCAC AGCTACACTC 
AGAGGAGATG TAACAGCAGT AACAAATAAT AAACATAACA AAATTGCAAC TGCAACAATA 
ATGCCAACAG CAACAACAAT AATAGCAACA ACAACGACAA TAAATCCAAA AATGCCAGCA 
GCAAGCAGCA CAAATGCTAC CCACACACAC ATACCCAGCT ATCAGAATTC CTATCACAGT 
GCCACACACA CAACCAACGC GTCACAAACA CAATCCACAC AGCCACAAAT GTTTGCGTCA 
CGCTACAAAA AAGACGATTA TCTGCCACAA TATCAACCAC AATATGCACA CGCGCAAACG 
CAGTTGTCAC ATCATCATCA TCAACCATCA CAACTACCTC AGCCGATTGC AAGCTATTAC 
AACAACAGCA ACGCCAACAA TCATTTACAG GGCGAACGTG GTGGAGGCGG TAGCATCAGC 
GGCAGCACCG GTGTAGGTCT GAGTAGTGGT GTCGGTGGCG GTGTCGGTGT CAGTGGTGGC 
GGCAATGGTT ATGGCAGCGG TGGTTCGGGC AGCAATAGCG CGAATAGCAG CAGTCCACAA 
TCGCCGCCCA GCAGCAGCTA CAAGGATGCA ACGCACAAGG ACGACACCAC GCATATTGTC 
AGCGAGATGA GCAAATTGTA TCGCAAATCA CCGTTTCTGC AGCGCAAATA TAGCAATGGC 
TCGGGTTTGA AGTACACCAG CGCGGTGGGC GGTGCCGACA GTCCCAAAAA GGAATCCATG 
TTCAGCAGTA TAGGTCAATC GATCATACGC AATCTCACGA CCTCGCCCTT TAGCCAAAAG 
AAACATCATA CACCACAACC ACATCAGGTG GATCCACTTT GGCGCATGCC GAATGTGCAG 
GAGATGGGTG GCAATGCGTT CGATGTGTAT GATAGTCCCG CCATACCGAA GACGGTGGCG 
TTGGGTTCGC CAGCGATGAC GCGCAAGCGT TTTCAGGGTG GCGCCGCATC ACCAACACTG 
CCCATAGGCA CGGCGGTGGA GCCAGTTTTG AAGGAGCAGC AGCCTTCGCT TATACCAGTT 
AATCGCGGTG AGCTGAATAC GCAGCAGCGC GTGCCCGGCA ACCATAGTCC AATAGTGCTG 
CAACGTTTCT ACCATCAGCA GAATCAGCTG CGTGAGAAGG AGTTGGAAAA GCGTAAGGAG 
CATCTGCAAC AACAAAAGCA CTCCACAAAT CCCTTCCTCG CTGGCAATTA TCTCTCCACA 
ACGGCTGCCG TCACAGCGTC CACTACGCCA TTGCATTACA GCAACACCAA CAACAGCGCC 
GGCGGCGTTG GCAATCAACA GCACTATCAA CTGCCCCAGC CGCAGCAGCA ACGCTATCCG 
CAACAGCAGC CAACGCATCA CATGCCGCTA ACCAGCGCTG CCTCCTCATC ACATTCAACT 
TCTAGTCAAT CATCCACACA ATCCTCGTCC TCACAAATAG CCCTAAGCGA TTTGCACGAA 
CATCAACAAG CGCGTTCGCC ACCCACTTGC GCACCACCGC CACCGCCGCA AACATCTGCC 
GCGGCAATCA GCACTGCCTT TGGCACGAGT CCCAGTCAGC TGCAATACCA ACCGCTGCCG 
CAGCATTATT TACCGTTGAA TGAGCATGCA GTACGTAGTT CGCGTTCGCC GCCTACCTCG 
CCGGAGCAGC TGTCTTCCGA TGGCGGTGTG AGCGGTAGTG GCGGTGGTGG TGGCAGCAGC 
GGCATGGCGA GCAAGTTGAG CAAATTGTAT GCGCGTCGGC AGTGGCAAAC GACCACGGCG 
TCAACGATAC CGACGCATAC AGGCGTTGAG GGTGTGTGTG GCAGTGGCGG AGGGATCGTT 
GGAGGCGGTG TTGGGTTGGC GGGCAAGGAA TACAGGGACG AACATGGCTG GTTTAACACA 
CTTGCCGGCG CCATGAAGCG TCAATTTGCC TCATATGTTA AATTACAATT GCATTCTGCA 
GGCTCGCCTT CGACCAGTGG CGCAGCCAAT CAACATGACG GCGTCGGCTA TGAGAGTGCG 
CTGCCACCGA GCGCACAACC TGGCAAACCG ACACTCGCCT ATTATCGTAC GCTCTCCGCG 
GGTAGCAGCA GTAATACGAG CAACAGCACT TCGCCGACTG AGGCGCAGCC TAAACCTGGA 
CAAACGGCGG GTAGTGACTT CTACGATGGC ACCAGCGGCT TTCTGCGTCG CTATGCCGCG 
GCAGGCGCGA GCGGCATGGT CAGCGGTTCT TCGAATGCGC ATCTGCTGAC CGGTTTGGAT 
CGGCGACACC GCAGTCCAGA TCCACCGCCG CGCTACAATC GCGGCCAATC GCCACTGCTG 
TTGCGCAAGC AGTTGCTGGA GCTGAGCGGC CAGCCGCCAG GCGGTTCACC GCTGCTCAAT 
AGACGCTATG TGAACGCCTC ACCACCATTG CCACCACCGC GTCGCGGCTC GGAGAGTGTG 
CCCGGCTCAC CGCAACATTT TCGCACGCGC ATTCATTACA CACCCGAACC ACAGCGGCGC 
ATATATCGCA CGATCGACCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011192403.1
CDS	1..3864
Translation	MHDAPEYKHSSSSSSREQHQQLGRVPVPNAAGTGARITPTTATHISSSSAVSKTSNSSMESIGHRIHRLEKRLEEENNSHNHLMFATDPPSGSATRVGARDVHMSLPIGSKSKRPILDLVMTNTNRNGPQTEMHKKLKRIRQQSGILTINGQKYPSRMSDLEHLSDLGNGTSGNVVKMRHKPTGTILAVKQMRSTGNDEENNRILMDLDVILNSHDCPYIVHCLGYFVNIPDVWICMELMSMCFDKLLRRSKQPVPENILGKVTVATVHALSYLKKNHNVIHRDVKPSNILIDEQGNIKLCDFGISGRLVDSKANTRSAGCAAYMAPERIDPKKPKYDIRADVWSLGITLVELATGRSPYEGCNIDFEVLTKILDHEPPSLPYGDGFDFSQEFRDFVDKCLTKNVHQRPRYQELLQQPFFRYYDAADVNVIQWFKTVVESADIEMHRTHMPITTTTTATLRGDVTAVTNNKHNKIATATIMPTATTIIATTTTINPKMPAASSTNATHTHIPSYQNSYHSATHTTNASQTQSTQPQMFASRYKKDDYLPQYQPQYAHAQTQLSHHHHQPSQLPQPIASYYNNSNANNHLQGERGGGGSISGSTGVGLSSGVGGGVGVSGGGNGYGSGGSGSNSANSSSPQSPPSSSYKDATHKDDTTHIVSEMSKLYRKSPFLQRKYSNGSGLKYTSAVGGADSPKKESMFSSIGQSIIRNLTTSPFSQKKHHTPQPHQVDPLWRMPNVQEMGGNAFDVYDSPAIPKTVALGSPAMTRKRFQGGAASPTLPIGTAVEPVLKEQQPSLIPVNRGELNTQQRVPGNHSPIVLQRFYHQQNQLREKELEKRKEHLQQQKHSTNPFLAGNYLSTTAAVTASTTPLHYSNTNNSAGGVGNQQHYQLPQPQQQRYPQQQPTHHMPLTSAASSSHSTSSQSSTQSSSSQIALSDLHEHQQARSPPTCAPPPPPQTSAAAISTAFGTSPSQLQYQPLPQHYLPLNEHAVRSSRSPPTSPEQLSSDGGVSGSGGGGGSSGMASKLSKLYARRQWQTTTASTIPTHTGVEGVCGSGGGIVGGGVGLAGKEYRDEHGWFNTLAGAMKRQFASYVKLQLHSAGSPSTSGAANQHDGVGYESALPPSAQPGKPTLAYYRTLSAGSSSNTSNSTSPTEAQPKPGQTAGSDFYDGTSGFLRRYAAAGASGMVSGSSNAHLLTGLDRRHRSPDPPPRYNRGQSPLLLRKQLLELSGQPPGGSPLLNRRYVNASPPLPPPRRGSESVPGSPQHFRTRIHYTPEPQRRIYRTIDQ

Target ORF information:

RefSeq Version	XM_011194101.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae dual specificity mitogen-activated protein kinase kinase hemipterous-like (LOC105218485), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011194101.2

ORF Insert Sequence:

ATGCACGACG CACCGGAGTA TAAACACAGC AGCAGTAGTA GCAGTCGTGA GCAACACCAA 
CAGCTAGGAA GAGTGCCAGT CCCCAACGCA GCAGGCACTG GAGCAAGGAT AACGCCAACC 
ACAGCTACGC ATATATCATC ATCATCAGCC GTTAGTAAAA CAAGCAATTC CTCAATGGAG 
TCCATTGGAC ATAGAATACA CAGACTAGAG AAACGTCTCG AGGAGGAGAA CAATTCACAC 
AATCACTTGA TGTTTGCTAC AGACCCTCCA AGTGGCTCTG CAACGCGTGT TGGCGCCAGA 
GATGTACACA TGTCGCTACC AATCGGCAGC AAGTCAAAAC GCCCCATCTT GGATTTGGTT 
ATGACAAATA CAAATCGTAA TGGTCCACAA ACAGAAATGC ACAAGAAACT TAAAAGAATA 
CGACAACAAT CCGGTATACT AACCATAAAT GGCCAAAAAT ATCCCAGTAG AATGTCTGAT 
TTGGAGCACT TGAGTGATTT GGGTAATGGC ACTAGTGGGA ATGTGGTAAA AATGCGACAC 
AAACCAACGG GCACGATTTT AGCCGTCAAA CAAATGCGTA GTACTGGCAA CGATGAAGAA 
AATAATCGTA TACTCATGGA TCTCGATGTT ATACTGAATT CACATGACTG CCCATATATA 
GTGCATTGTT TAGGTTATTT TGTAAATATA CCGGATGTGT GGATATGCAT GGAACTGATG 
TCAATGTGTT TCGATAAGCT GCTAAGGCGT TCAAAACAAC CGGTGCCAGA GAATATACTT 
GGGAAGGTTA CTGTAGCGAC GGTGCACGCT CTTTCATATT TAAAAAAGAA TCACAATGTC 
ATACATCGAG ATGTGAAACC TTCAAATATC CTCATCGACG AACAGGGCAA TATCAAATTG 
TGCGATTTCG GCATTAGCGG GCGACTTGTG GATTCAAAAG CGAATACACG TTCAGCCGGG 
TGCGCCGCTT ACATGGCGCC TGAACGCATC GATCCGAAAA AGCCGAAATA TGATATACGC 
GCTGATGTTT GGTCATTGGG CATAACACTA GTGGAACTTG CAACCGGCCG TTCACCGTAC 
GAAGGCTGCA ACATAGACTT TGAGGTGCTG ACAAAAATTT TGGATCACGA ACCACCCAGT 
TTGCCATATG GTGATGGTTT TGATTTCAGT CAAGAATTTC GTGATTTTGT CGATAAATGT 
CTGACGAAAA ATGTACATCA GCGCCCCAGA TACCAGGAGC TCTTGCAGCA ACCATTTTTT 
CGTTATTACG ACGCGGCTGA TGTGAATGTG ATTCAATGGT TTAAGACTGT TGTCGAATCT 
GCCGACATAG AGATGCATAG AACTCACATG CCAATTACTA CAACAACCAC AGCTACACTC 
AGAGGAGATG TAACAGCAGT AACAAATAAT AAACATAACA AAATTGCAAC TGCAACAATA 
ATGCCAACAG CAACAACAAT AATAGCAACA ACAACGACAA TAAATCCAAA AATGCCAGCA 
GCAAGCAGCA CAAATGCTAC CCACACACAC ATACCCAGCT ATCAGAATTC CTATCACAGT 
GCCACACACA CAACCAACGC GTCACAAACA CAATCCACAC AGCCACAAAT GTTTGCGTCA 
CGCTACAAAA AAGACGATTA TCTGCCACAA TATCAACCAC AATATGCACA CGCGCAAACG 
CAGTTGTCAC ATCATCATCA TCAACCATCA CAACTACCTC AGCCGATTGC AAGCTATTAC 
AACAACAGCA ACGCCAACAA TCATTTACAG GGCGAACGTG GTGGAGGCGG TAGCATCAGC 
GGCAGCACCG GTGTAGGTCT GAGTAGTGGT GTCGGTGGCG GTGTCGGTGT CAGTGGTGGC 
GGCAATGGTT ATGGCAGCGG TGGTTCGGGC AGCAATAGCG CGAATAGCAG CAGTCCACAA 
TCGCCGCCCA GCAGCAGCTA CAAGGATGCA ACGCACAAGG ACGACACCAC GCATATTGTC 
AGCGAGATGA GCAAATTGTA TCGCAAATCA CCGTTTCTGC AGCGCAAATA TAGCAATGGC 
TCGGGTTTGA AGTACACCAG CGCGGTGGGC GGTGCCGACA GTCCCAAAAA GGAATCCATG 
TTCAGCAGTA TAGGTCAATC GATCATACGC AATCTCACGA CCTCGCCCTT TAGCCAAAAG 
AAACATCATA CACCACAACC ACATCAGGTG GATCCACTTT GGCGCATGCC GAATGTGCAG 
GAGATGGGTG GCAATGCGTT CGATGTGTAT GATAGTCCCG CCATACCGAA GACGGTGGCG 
TTGGGTTCGC CAGCGATGAC GCGCAAGCGT TTTCAGGGTG GCGCCGCATC ACCAACACTG 
CCCATAGGCA CGGCGGTGGA GCCAGTTTTG AAGGAGCAGC AGCCTTCGCT TATACCAGTT 
AATCGCGGTG AGCTGAATAC GCAGCAGCGC GTGCCCGGCA ACCATAGTCC AATAGTGCTG 
CAACGTTTCT ACCATCAGCA GAATCAGCTG CGTGAGAAGG AGTTGGAAAA GCGTAAGGAG 
CATCTGCAAC AACAAAAGCA CTCCACAAAT CCCTTCCTCG CTGGCAATTA TCTCTCCACA 
ACGGCTGCCG TCACAGCGTC CACTACGCCA TTGCATTACA GCAACACCAA CAACAGCGCC 
GGCGGCGTTG GCAATCAACA GCACTATCAA CTGCCCCAGC CGCAGCAGCA ACGCTATCCG 
CAACAGCAGC CAACGCATCA CATGCCGCTA ACCAGCGCTG CCTCCTCATC ACATTCAACT 
TCTAGTCAAT CATCCACACA ATCCTCGTCC TCACAAATAG CCCTAAGCGA TTTGCACGAA 
CATCAACAAG CGCGTTCGCC ACCCACTTGC GCACCACCGC CACCGCCGCA AACATCTGCC 
GCGGCAATCA GCACTGCCTT TGGCACGAGT CCCAGTCAGC TGCAATACCA ACCGCTGCCG 
CAGCATTATT TACCGTTGAA TGAGCATGCA GTACGTAGTT CGCGTTCGCC GCCTACCTCG 
CCGGAGCAGC TGTCTTCCGA TGGCGGTGTG AGCGGTAGTG GCGGTGGTGG TGGCAGCAGC 
GGCATGGCGA GCAAGTTGAG CAAATTGTAT GCGCGTCGGC AGTGGCAAAC GACCACGGCG 
TCAACGATAC CGACGCATAC AGGCGTTGAG GGTGTGTGTG GCAGTGGCGG AGGGATCGTT 
GGAGGCGGTG TTGGGTTGGC GGGCAAGGAA TACAGGGACG AACATGGCTG GTTTAACACA 
CTTGCCGGCG CCATGAAGCG TCAATTTGCC TCATATGTTA AATTACAATT GCATTCTGCA 
GGCTCGCCTT CGACCAGTGG CGCAGCCAAT CAACATGACG GCGTCGGCTA TGAGAGTGCG 
CTGCCACCGA GCGCACAACC TGGCAAACCG ACACTCGCCT ATTATCGTAC GCTCTCCGCG 
GGTAGCAGCA GTAATACGAG CAACAGCACT TCGCCGACTG AGGCGCAGCC TAAACCTGGA 
CAAACGGCGG GTAGTGACTT CTACGATGGC ACCAGCGGCT TTCTGCGTCG CTATGCCGCG 
GCAGGCGCGA GCGGCATGGT CAGCGGTTCT TCGAATGCGC ATCTGCTGAC CGGTTTGGAT 
CGGCGACACC GCAGTCCAGA TCCACCGCCG CGCTACAATC GCGGCCAATC GCCACTGCTG 
TTGCGCAAGC AGTTGCTGGA GCTGAGCGGC CAGCCGCCAG GCGGTTCACC GCTGCTCAAT 
AGACGCTATG TGAACGCCTC ACCACCATTG CCACCACCGC GTCGCGGCTC GGAGAGTGTG 
CCCGGCTCAC CGCAACATTT TCGCACGCGC ATTCATTACA CACCCGAACC ACAGCGGCGC 
ATATATCGCA CGATCGACCA ATGA