LOC105218422 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105218422
Entrez Gene ID	105218422
Full Name	endochitinase
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011193984.1	XP_011192286.1	endochitinase

Pathways from BioSystems
Gene Ontology

The following LOC105218422 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105218422 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa205566	XM_011193984.1 Latest version!	Zeugodacus cucurbitae endochitinase (LOC105218422), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105218422 ( XM_011193984.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011192286.1 Zeugodacus cucurbitae endochitinase (LOC105218422), mRNA.

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CloneID	OBa205566
Clone ID Related Accession (Same CDS sequence)	XM_011193984.1
Accession Version	XM_011193984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	endochitinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863859.1) and transcript sequence (GBXI01003504.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 297 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGATATAC CTTTAAAGCT ATTACTACTT AGCTTAACGC TAACGGCGGC ACTTACGGGT 
ACAGCTGCCA ACGAGCAGCC AGCACGCATT GTTTGCTACT TCAGCAATTG GGCCGTCTAC 
CGGCCCGGTG TGGGACGTTA TGGACTCGAA GATGTGCCCG CTGACTTGTG CACACATTTG 
GTCTACTCAT TCATTGGTTT AAACGACAAG AGTTGGGACG TCTTGGTAAT CGATCCCGAA 
TTGGATGTGG AACAAGAGGG CTTCCGCAAG TTCACGCAAT TGCGACAGAA ACATCCAAAC 
TTAAAGCTGC AGGTGGCCGT CGGCGGCTGG GCAGAGGGTG GCTCGAAGTA TTCACAAATG 
GTGGCGGTGC GTGAGCGACG ACTCAGTTTC ATACGCAGCG TGGTGCAGTT CATGAAGGAA 
TATGATTTCG ATGGTTTCGA TTTGGATTGG GAATACCCTG GCGCTACTGA TCGCGGTGGC 
ACTTTTGGCG ATAAAGACAA ATTCTTATAC TTCGTGCAAG AGTTGCGACG CGCATTCGAT 
CGTGAGGGAC GCGGTTGGGA AATAACAATG GCGGTGCCGG TGGCTAAATT CCGTTTGCAG 
GAAGGTTATC ATGTGCCTGA GCTGTGCGAA TTGCTTGATG CCATACACGC CATGACTTAT 
GATCTGCGCG GCAACTGGGC CGGTTTCGCC GATGTACACA GTCCGCTCTA CAAGCGCAAG 
CACGATCAGT GGGCTTATGA GAAACTGAAT GTGAATGATG GTCTAGGTCT CTGGGAGGAG 
ATGGGTTGCC CTGCCAATAA GCTGGTGGTC GGCGTGCCCT TCTATGGACG CACTTACACG 
CTGAGCAACT CCAACAAGAA CTACAACATG GGCACTTACA TCAATAAGGA GGCTGGCGGT 
GGCGCACCAG GCCCTTACAC CAATGCTAGC GGTTTCCTTG CTTACTATGA GATATGTACC 
GAGGTACAGG ACGCAGACAA GGGTTGGACC GTGCAGTGGG ATGAACAAGG CATGGTGCCC 
TACACCTACA AAGACACACA ATGGGTGGGC TATGAGGATG AGAAATCGGT GCAAATCAAA 
ATGGACTATA TCAAGCAGAA GGGCTACGCG GGCGCTATGA CTTGGGCCAT TGATATGGAC 
GATTTCCATG GTTTGTGTGG GCGCGAGAAT GCGCTGATGC AAATTCTCTA TGACAACATG 
CACGATTACA CGGTGCCGGA GCCTACGCGC GAGACGACGC CGCGGCCCGA GTGGGCCAAA 
CCACCAGCGA CGCCGCCGAA TCCCGACGAG GCCAGTTTCG TCTCACAAGA TGTCACCACA 
AAACGGCCGA AGCCAAAGCC AACAGCAGCG ACCAGCACAA CAAAACGACC GCTCGCCGAA 
CAAACAACGA CAACAACAAC CAAGAAACCG TCCAAAGGCT CCAAGAAACC CAAACCGAGA 
CCAAAGCCCA GACCCACATC TTCCACGACA ACAACAACAA CAGAAACACC AACATCATCA 
AGCAGCGAAG AAACTGGTGG CTTGGAAGTA TTAGAACCAA AACCAGAGGT TGTAGAACCA 
ACCGGTGGAC ACGTGATGCC CGACATTGAT TGTACAAACA AGGACTTTAT ACCGCATGCA 
GATTGTCAAA AGTACTACCG CTGCGTTCAC GGCGAACCAG TCGAATTCGA GTGCAAGGAA 
GGCACCGCTT TCCATACAAT TCTGAATGTT TGCGATTGGA TTGAGAATAG CGACCGTTAT 
TACTGCACCA AAACGAAGAA GAAGGCGCTG AGCAATGAGA CGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011192286.1
CDS	217..2004
Translation	MDIPLKLLLLSLTLTAALTGTAANEQPARIVCYFSNWAVYRPGVGRYGLEDVPADLCTHLVYSFIGLNDKSWDVLVIDPELDVEQEGFRKFTQLRQKHPNLKLQVAVGGWAEGGSKYSQMVAVRERRLSFIRSVVQFMKEYDFDGFDLDWEYPGATDRGGTFGDKDKFLYFVQELRRAFDREGRGWEITMAVPVAKFRLQEGYHVPELCELLDAIHAMTYDLRGNWAGFADVHSPLYKRKHDQWAYEKLNVNDGLGLWEEMGCPANKLVVGVPFYGRTYTLSNSNKNYNMGTYINKEAGGGAPGPYTNASGFLAYYEICTEVQDADKGWTVQWDEQGMVPYTYKDTQWVGYEDEKSVQIKMDYIKQKGYAGAMTWAIDMDDFHGLCGRENALMQILYDNMHDYTVPEPTRETTPRPEWAKPPATPPNPDEASFVSQDVTTKRPKPKPTAATSTTKRPLAEQTTTTTTKKPSKGSKKPKPRPKPRPTSSTTTTTTETPTSSSSEETGGLEVLEPKPEVVEPTGGHVMPDIDCTNKDFIPHADCQKYYRCVHGEPVEFECKEGTAFHTILNVCDWIENSDRYYCTKTKKKALSNETK

Target ORF information:

RefSeq Version	XM_011193984.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae endochitinase (LOC105218422), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193984.1

ORF Insert Sequence:

ATGGATATAC CTTTAAAGCT ATTACTACTT AGCTTAACGC TAACGGCGGC ACTTACGGGT 
ACAGCTGCCA ACGAGCAGCC AGCACGCATT GTTTGCTACT TCAGCAATTG GGCCGTCTAC 
CGGCCCGGTG TGGGACGTTA TGGACTCGAA GATGTGCCCG CTGACTTGTG CACACATTTG 
GTCTACTCAT TCATTGGTTT AAACGACAAG AGTTGGGACG TCTTGGTAAT CGATCCCGAA 
TTGGATGTGG AACAAGAGGG CTTCCGCAAG TTCACGCAAT TGCGACAGAA ACATCCAAAC 
TTAAAGCTGC AGGTGGCCGT CGGCGGCTGG GCAGAGGGTG GCTCGAAGTA TTCACAAATG 
GTGGCGGTGC GTGAGCGACG ACTCAGTTTC ATACGCAGCG TGGTGCAGTT CATGAAGGAA 
TATGATTTCG ATGGTTTCGA TTTGGATTGG GAATACCCTG GCGCTACTGA TCGCGGTGGC 
ACTTTTGGCG ATAAAGACAA ATTCTTATAC TTCGTGCAAG AGTTGCGACG CGCATTCGAT 
CGTGAGGGAC GCGGTTGGGA AATAACAATG GCGGTGCCGG TGGCTAAATT CCGTTTGCAG 
GAAGGTTATC ATGTGCCTGA GCTGTGCGAA TTGCTTGATG CCATACACGC CATGACTTAT 
GATCTGCGCG GCAACTGGGC CGGTTTCGCC GATGTACACA GTCCGCTCTA CAAGCGCAAG 
CACGATCAGT GGGCTTATGA GAAACTGAAT GTGAATGATG GTCTAGGTCT CTGGGAGGAG 
ATGGGTTGCC CTGCCAATAA GCTGGTGGTC GGCGTGCCCT TCTATGGACG CACTTACACG 
CTGAGCAACT CCAACAAGAA CTACAACATG GGCACTTACA TCAATAAGGA GGCTGGCGGT 
GGCGCACCAG GCCCTTACAC CAATGCTAGC GGTTTCCTTG CTTACTATGA GATATGTACC 
GAGGTACAGG ACGCAGACAA GGGTTGGACC GTGCAGTGGG ATGAACAAGG CATGGTGCCC 
TACACCTACA AAGACACACA ATGGGTGGGC TATGAGGATG AGAAATCGGT GCAAATCAAA 
ATGGACTATA TCAAGCAGAA GGGCTACGCG GGCGCTATGA CTTGGGCCAT TGATATGGAC 
GATTTCCATG GTTTGTGTGG GCGCGAGAAT GCGCTGATGC AAATTCTCTA TGACAACATG 
CACGATTACA CGGTGCCGGA GCCTACGCGC GAGACGACGC CGCGGCCCGA GTGGGCCAAA 
CCACCAGCGA CGCCGCCGAA TCCCGACGAG GCCAGTTTCG TCTCACAAGA TGTCACCACA 
AAACGGCCGA AGCCAAAGCC AACAGCAGCG ACCAGCACAA CAAAACGACC GCTCGCCGAA 
CAAACAACGA CAACAACAAC CAAGAAACCG TCCAAAGGCT CCAAGAAACC CAAACCGAGA 
CCAAAGCCCA GACCCACATC TTCCACGACA ACAACAACAA CAGAAACACC AACATCATCA 
AGCAGCGAAG AAACTGGTGG CTTGGAAGTA TTAGAACCAA AACCAGAGGT TGTAGAACCA 
ACCGGTGGAC ACGTGATGCC CGACATTGAT TGTACAAACA AGGACTTTAT ACCGCATGCA 
GATTGTCAAA AGTACTACCG CTGCGTTCAC GGCGAACCAG TCGAATTCGA GTGCAAGGAA 
GGCACCGCTT TCCATACAAT TCTGAATGTT TGCGATTGGA TTGAGAATAG CGACCGTTAT 
TACTGCACCA AAACGAAGAA GAAGGCGCTG AGCAATGAGA CGAAGTAA