Orc1_0 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Orc1_0
Entrez Gene ID	105217365
Full Name	GPI ethanolamine phosphate transferase 2
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011192323.1	XP_011190625.1	GPI ethanolamine phosphate transferase 2
XM_011192323.2	XP_011190625.1	GPI ethanolamine phosphate transferase 2

Pathways from BioSystems
Gene Ontology

The following Orc1_0 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Orc1_0 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa204391	XM_011192323.2 Latest version!	Zeugodacus cucurbitae GPI ethanolamine phosphate transferase 2 (Orc1_0), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa204391	XM_011192323.1	Bactrocera cucurbitae GPI ethanolamine phosphate transferase 2 (LOC105217365), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Orc1_0 ( XM_011192323.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011190625.1 Bactrocera cucurbitae GPI ethanolamine phosphate transferase 2 (LOC105217365), mRNA.

Price & Availability↑

CloneID	OBa204391
Clone ID Related Accession (Same CDS sequence)	XM_011192323.1 , XM_011192323.2
Accession Version	XM_011192323.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	GPI ethanolamine phosphate transferase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863816.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAC AGATGGAAAA ATCAAGACCT ATTAGAATTT TTCTAATATG CATGATAGTT 
TCGTTTATTT TTGGCGCTTC AATTTTTCTA ACTGGTTTCT TTCCCATAAA CAATTATGCT 
TTGGATCGTG TTGCCGCTGA AAGCAAACCA AAAGATTTGG ATGGTTTGAG TTTGACACCG 
CCAACTCAAA TTTACGCACA AACCATACTT ATGCTGGTGG ATGCGCTACG TTTCGATTTT 
GCCAGTCAAA ATGGAATTCC TTTCTCCTAC AACAACTCAT GTAATCGTCT CAAGTTGCGT 
GTTAACATAC CCACTGTTAC CATGCCTCGT CTTAAAAGCT TAACCACCGG CACGGTTTCA 
AATTTTATTG ATATTATCCT AAATATTGGT CATGTTGAAC AACTTACAGA CTCTTTGTTA 
CACCGCTTAC AAGAACGTAA CGAAACTACG GTTTTTGCTG GTGACCGTAC TTGGATAAGT 
CTATTTCCAA AACAATTTAC ACGCTTTACC GCTAACTCAG ATTCGTTTTT TGTGAACGAT 
TTTTATGAGG GTGACAAAAA TGTGACGGAA GTATTATCTA AAGAATTGCT GAATAATGAC 
TGGATGTTGT TGGTGCTACA TTATTTGGGT TTAGATCATA TTGGACATGT TGAAGGTAGT 
GAAAGTGCCA AAATCCCGCT TAAATTAAAA GAAATGGATG AAGTTGTGCT GAAGCTATAC 
AATGCAAAGC ATTTTCACAG ACAGCTGCTT TTATTAACTG GTGACCATGG TATGCGTGAT 
GGTGGCGGTC ATGGTGGGAG TACAACGGGT GAGATGTATG TGCCATTATT TGTTTTCAAA 
GAAAACTGCA CCAAAAGCAG TGCTAGTAAA TCTAACGAGT ATAATCAAAT TGATATGGCG 
CCCACATTAG CCATACTCTG GTCCATGGAA ATTCCGCCGA TGTCAATTGG CTGTTTAATA 
CCCGAGCTCT TGCATGAGTT TAACATGGAG GACCAACTAT ACGCTTATTA TTATAATGCG 
CTACATTTAG TGCAAAAAGC TAACAGTAAA TTTGATGAAG ATTTTGTTAA TAACAATGCA 
TTCAACAAAT GGTTTACCAC AGCCAAATCG GCGCATAAGC AGTTTCTTTT AGAAAAACGC 
AATAAAAACT TTGAAAATAC TTTTATTTTC GAGCACGCTA AAGTGCATTA CTTACGTTTG 
GCACGCGAAA TTTCAAACCA ACTATCCGAC TCATTAGTGC AATTCGATTA CGGGCTGATC 
ACACTGGGGC TTATGCTAAC CACATTTGTC ACACTCAACG CACTTTTGGC TTTTTTCACA 
ACCACTGCAT GTGTTATTAT CAAAGGCAAG GCAGCATGGC TGTGTCTATT AGCTTTCGCC 
GCATGCATAA ATAAATGGTG CCACTACCAG CGTTACTTTT TGTCTACTGG CATAATGTTT 
ACCATTACGA TATTGTTGGC CATAATGGCT AACACTTATT TGCTTATAAA TATAGTGTAT 
ATATTATTTC GCCGATTACA TATGAGCTGT ACACTAGCAC AATCCTCACT GCCCAAACCA 
AAACAAATTT GGTTGTTGTT AAGTTGTTTA GTATTTCATA CGCTTACGCT GGCCTCCTCA 
TCGTACATTG AATACGAGAA TATAACTTGG CATTATCTTG GAGCCAGCGC TATATTTTTG 
CTTTTTGTGC AAAATTTTCA AACACAGTTA TTTGCCTGCA GGCTGGACAC ATGCGTCAAT 
GATATGGCGC TGTATAAAGT TGTTTTGCGA AATTTGCTAA AATCACACAC GTCCCTATTG 
GTGATTGGTG GTTTGGGTAT TTTGTACCGT TTAGACGGTG CGTCGTCTTA TCTAACGCAG 
GAGCGAAATA AAGCATGGCT AAGTTTCATA TTTGGTGCAG GCCTGATTTT ATATACGCTA 
TGTTTACGTC AGCATAGTCT TGTGCAAACG CGTAAGGAGC TTGTGTTGTA CTTCTTCGCT 
GCGATGCTGA TCTATTGCTA TCGTGCCTTG AAGGGAGTGG TTTACTTCGT GTTCAACACA 
AACTGCTGTG ATAATATTGT TTTGAACATA TTTTGGCTGT TGGTCGTTAT TGCTGCATGC 
ATATCAACTG TGATATTACA TTCCAAACGC CGTATTAGTT ATCAAATTCA GCACACTTCG 
TTAGCGGGTT GCTTTAAAAT TCTACTAACT TTACTGCTGA CCGTTTCCTT GTTGCTGCAT 
AAGCCACACA ATGTTCTTCT AGTCGCTTAT TTAATATATA CGCTCTCTTT GTGCTATGAG 
TTGTGCGATA AATGCGAAAA GAACGCGCTA CGCAATTACA AATACAAGTA CTTATCGAAG 
CTGCTGTGCA CTATACTTGT GGGCAATATG TTTTACTTCT ATCAGGGTAA TTCGAATAGT 
TTTGCCACGA TCGATCTAAA TCCCGGCTAC ATTGGTCAAA CTACTTATAA TCCAGTAGTT 
GTCGGTATAC TTGTCACCTT GAATATGTAT TCAGCGCAAT TTATTGCATT TTTATACTTA 
ATTACGCATA TTTTGCAAGA AAGTGTTAAC AAAACAAACG GTTTGTCTGC AAATGAGCAT 
ATAAATTTCA TTATTTATTT CTACACCTTA AGCATATTTG TACCGGTTTT AATTTATTTC 
TGGTTACTGT TACTCTTTCG TTACCATCTG TTTATCTATT CAGTATTTGC GCCGAAAGCA 
TTTTATGAAT ACTACCGTGT ATTTGTACTT TTCTTTACAT ATTTAATGAC AACAATTAAC 
TTGTACATAT TCGATCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011190625.1
CDS	104..2884
Translation	MALQMEKSRPIRIFLICMIVSFIFGASIFLTGFFPINNYALDRVAAESKPKDLDGLSLTPPTQIYAQTILMLVDALRFDFASQNGIPFSYNNSCNRLKLRVNIPTVTMPRLKSLTTGTVSNFIDIILNIGHVEQLTDSLLHRLQERNETTVFAGDRTWISLFPKQFTRFTANSDSFFVNDFYEGDKNVTEVLSKELLNNDWMLLVLHYLGLDHIGHVEGSESAKIPLKLKEMDEVVLKLYNAKHFHRQLLLLTGDHGMRDGGGHGGSTTGEMYVPLFVFKENCTKSSASKSNEYNQIDMAPTLAILWSMEIPPMSIGCLIPELLHEFNMEDQLYAYYYNALHLVQKANSKFDEDFVNNNAFNKWFTTAKSAHKQFLLEKRNKNFENTFIFEHAKVHYLRLAREISNQLSDSLVQFDYGLITLGLMLTTFVTLNALLAFFTTTACVIIKGKAAWLCLLAFAACINKWCHYQRYFLSTGIMFTITILLAIMANTYLLINIVYILFRRLHMSCTLAQSSLPKPKQIWLLLSCLVFHTLTLASSSYIEYENITWHYLGASAIFLLFVQNFQTQLFACRLDTCVNDMALYKVVLRNLLKSHTSLLVIGGLGILYRLDGASSYLTQERNKAWLSFIFGAGLILYTLCLRQHSLVQTRKELVLYFFAAMLIYCYRALKGVVYFVFNTNCCDNIVLNIFWLLVVIAACISTVILHSKRRISYQIQHTSLAGCFKILLTLLLTVSLLLHKPHNVLLVAYLIYTLSLCYELCDKCEKNALRNYKYKYLSKLLCTILVGNMFYFYQGNSNSFATIDLNPGYIGQTTYNPVVVGILVTLNMYSAQFIAFLYLITHILQESVNKTNGLSANEHINFIIYFYTLSIFVPVLIYFWLLLLFRYHLFIYSVFAPKAFYEYYRVFVLFFTYLMTTINLYIFDQ

Target ORF information:

RefSeq Version	XM_011192323.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae GPI ethanolamine phosphate transferase 2 (LOC105217365), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192323.1

ORF Insert Sequence:

ATGGCATTAC AGATGGAAAA ATCAAGACCT ATTAGAATTT TTCTAATATG CATGATAGTT 
TCGTTTATTT TTGGCGCTTC AATTTTTCTA ACTGGTTTCT TTCCCATAAA CAATTATGCT 
TTGGATCGTG TTGCCGCTGA AAGCAAACCA AAAGATTTGG ATGGTTTGAG TTTGACACCG 
CCAACTCAAA TTTACGCACA AACCATACTT ATGCTGGTGG ATGCGCTACG TTTCGATTTT 
GCCAGTCAAA ATGGAATTCC TTTCTCCTAC AACAACTCAT GTAATCGTCT CAAGTTGCGT 
GTTAACATAC CCACTGTTAC CATGCCTCGT CTTAAAAGCT TAACCACCGG CACGGTTTCA 
AATTTTATTG ATATTATCCT AAATATTGGT CATGTTGAAC AACTTACAGA CTCTTTGTTA 
CACCGCTTAC AAGAACGTAA CGAAACTACG GTTTTTGCTG GTGACCGTAC TTGGATAAGT 
CTATTTCCAA AACAATTTAC ACGCTTTACC GCTAACTCAG ATTCGTTTTT TGTGAACGAT 
TTTTATGAGG GTGACAAAAA TGTGACGGAA GTATTATCTA AAGAATTGCT GAATAATGAC 
TGGATGTTGT TGGTGCTACA TTATTTGGGT TTAGATCATA TTGGACATGT TGAAGGTAGT 
GAAAGTGCCA AAATCCCGCT TAAATTAAAA GAAATGGATG AAGTTGTGCT GAAGCTATAC 
AATGCAAAGC ATTTTCACAG ACAGCTGCTT TTATTAACTG GTGACCATGG TATGCGTGAT 
GGTGGCGGTC ATGGTGGGAG TACAACGGGT GAGATGTATG TGCCATTATT TGTTTTCAAA 
GAAAACTGCA CCAAAAGCAG TGCTAGTAAA TCTAACGAGT ATAATCAAAT TGATATGGCG 
CCCACATTAG CCATACTCTG GTCCATGGAA ATTCCGCCGA TGTCAATTGG CTGTTTAATA 
CCCGAGCTCT TGCATGAGTT TAACATGGAG GACCAACTAT ACGCTTATTA TTATAATGCG 
CTACATTTAG TGCAAAAAGC TAACAGTAAA TTTGATGAAG ATTTTGTTAA TAACAATGCA 
TTCAACAAAT GGTTTACCAC AGCCAAATCG GCGCATAAGC AGTTTCTTTT AGAAAAACGC 
AATAAAAACT TTGAAAATAC TTTTATTTTC GAGCACGCTA AAGTGCATTA CTTACGTTTG 
GCACGCGAAA TTTCAAACCA ACTATCCGAC TCATTAGTGC AATTCGATTA CGGGCTGATC 
ACACTGGGGC TTATGCTAAC CACATTTGTC ACACTCAACG CACTTTTGGC TTTTTTCACA 
ACCACTGCAT GTGTTATTAT CAAAGGCAAG GCAGCATGGC TGTGTCTATT AGCTTTCGCC 
GCATGCATAA ATAAATGGTG CCACTACCAG CGTTACTTTT TGTCTACTGG CATAATGTTT 
ACCATTACGA TATTGTTGGC CATAATGGCT AACACTTATT TGCTTATAAA TATAGTGTAT 
ATATTATTTC GCCGATTACA TATGAGCTGT ACACTAGCAC AATCCTCACT GCCCAAACCA 
AAACAAATTT GGTTGTTGTT AAGTTGTTTA GTATTTCATA CGCTTACGCT GGCCTCCTCA 
TCGTACATTG AATACGAGAA TATAACTTGG CATTATCTTG GAGCCAGCGC TATATTTTTG 
CTTTTTGTGC AAAATTTTCA AACACAGTTA TTTGCCTGCA GGCTGGACAC ATGCGTCAAT 
GATATGGCGC TGTATAAAGT TGTTTTGCGA AATTTGCTAA AATCACACAC GTCCCTATTG 
GTGATTGGTG GTTTGGGTAT TTTGTACCGT TTAGACGGTG CGTCGTCTTA TCTAACGCAG 
GAGCGAAATA AAGCATGGCT AAGTTTCATA TTTGGTGCAG GCCTGATTTT ATATACGCTA 
TGTTTACGTC AGCATAGTCT TGTGCAAACG CGTAAGGAGC TTGTGTTGTA CTTCTTCGCT 
GCGATGCTGA TCTATTGCTA TCGTGCCTTG AAGGGAGTGG TTTACTTCGT GTTCAACACA 
AACTGCTGTG ATAATATTGT TTTGAACATA TTTTGGCTGT TGGTCGTTAT TGCTGCATGC 
ATATCAACTG TGATATTACA TTCCAAACGC CGTATTAGTT ATCAAATTCA GCACACTTCG 
TTAGCGGGTT GCTTTAAAAT TCTACTAACT TTACTGCTGA CCGTTTCCTT GTTGCTGCAT 
AAGCCACACA ATGTTCTTCT AGTCGCTTAT TTAATATATA CGCTCTCTTT GTGCTATGAG 
TTGTGCGATA AATGCGAAAA GAACGCGCTA CGCAATTACA AATACAAGTA CTTATCGAAG 
CTGCTGTGCA CTATACTTGT GGGCAATATG TTTTACTTCT ATCAGGGTAA TTCGAATAGT 
TTTGCCACGA TCGATCTAAA TCCCGGCTAC ATTGGTCAAA CTACTTATAA TCCAGTAGTT 
GTCGGTATAC TTGTCACCTT GAATATGTAT TCAGCGCAAT TTATTGCATT TTTATACTTA 
ATTACGCATA TTTTGCAAGA AAGTGTTAAC AAAACAAACG GTTTGTCTGC AAATGAGCAT 
ATAAATTTCA TTATTTATTT CTACACCTTA AGCATATTTG TACCGGTTTT AATTTATTTC 
TGGTTACTGT TACTCTTTCG TTACCATCTG TTTATCTATT CAGTATTTGC GCCGAAAGCA 
TTTTATGAAT ACTACCGTGT ATTTGTACTT TTCTTTACAT ATTTAATGAC AACAATTAAC 
TTGTACATAT TCGATCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Orc1_0 ( XM_011192323.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011190625.1 Zeugodacus cucurbitae GPI ethanolamine phosphate transferase 2 (Orc1_0), mRNA.

Price & Availability↑

CloneID	OBa204391
Clone ID Related Accession (Same CDS sequence)	XM_011192323.1 , XM_011192323.2
Accession Version	XM_011192323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	GPI ethanolamine phosphate transferase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863816.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011192323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAC AGATGGAAAA ATCAAGACCT ATTAGAATTT TTCTAATATG CATGATAGTT 
TCGTTTATTT TTGGCGCTTC AATTTTTCTA ACTGGTTTCT TTCCCATAAA CAATTATGCT 
TTGGATCGTG TTGCCGCTGA AAGCAAACCA AAAGATTTGG ATGGTTTGAG TTTGACACCG 
CCAACTCAAA TTTACGCACA AACCATACTT ATGCTGGTGG ATGCGCTACG TTTCGATTTT 
GCCAGTCAAA ATGGAATTCC TTTCTCCTAC AACAACTCAT GTAATCGTCT CAAGTTGCGT 
GTTAACATAC CCACTGTTAC CATGCCTCGT CTTAAAAGCT TAACCACCGG CACGGTTTCA 
AATTTTATTG ATATTATCCT AAATATTGGT CATGTTGAAC AACTTACAGA CTCTTTGTTA 
CACCGCTTAC AAGAACGTAA CGAAACTACG GTTTTTGCTG GTGACCGTAC TTGGATAAGT 
CTATTTCCAA AACAATTTAC ACGCTTTACC GCTAACTCAG ATTCGTTTTT TGTGAACGAT 
TTTTATGAGG GTGACAAAAA TGTGACGGAA GTATTATCTA AAGAATTGCT GAATAATGAC 
TGGATGTTGT TGGTGCTACA TTATTTGGGT TTAGATCATA TTGGACATGT TGAAGGTAGT 
GAAAGTGCCA AAATCCCGCT TAAATTAAAA GAAATGGATG AAGTTGTGCT GAAGCTATAC 
AATGCAAAGC ATTTTCACAG ACAGCTGCTT TTATTAACTG GTGACCATGG TATGCGTGAT 
GGTGGCGGTC ATGGTGGGAG TACAACGGGT GAGATGTATG TGCCATTATT TGTTTTCAAA 
GAAAACTGCA CCAAAAGCAG TGCTAGTAAA TCTAACGAGT ATAATCAAAT TGATATGGCG 
CCCACATTAG CCATACTCTG GTCCATGGAA ATTCCGCCGA TGTCAATTGG CTGTTTAATA 
CCCGAGCTCT TGCATGAGTT TAACATGGAG GACCAACTAT ACGCTTATTA TTATAATGCG 
CTACATTTAG TGCAAAAAGC TAACAGTAAA TTTGATGAAG ATTTTGTTAA TAACAATGCA 
TTCAACAAAT GGTTTACCAC AGCCAAATCG GCGCATAAGC AGTTTCTTTT AGAAAAACGC 
AATAAAAACT TTGAAAATAC TTTTATTTTC GAGCACGCTA AAGTGCATTA CTTACGTTTG 
GCACGCGAAA TTTCAAACCA ACTATCCGAC TCATTAGTGC AATTCGATTA CGGGCTGATC 
ACACTGGGGC TTATGCTAAC CACATTTGTC ACACTCAACG CACTTTTGGC TTTTTTCACA 
ACCACTGCAT GTGTTATTAT CAAAGGCAAG GCAGCATGGC TGTGTCTATT AGCTTTCGCC 
GCATGCATAA ATAAATGGTG CCACTACCAG CGTTACTTTT TGTCTACTGG CATAATGTTT 
ACCATTACGA TATTGTTGGC CATAATGGCT AACACTTATT TGCTTATAAA TATAGTGTAT 
ATATTATTTC GCCGATTACA TATGAGCTGT ACACTAGCAC AATCCTCACT GCCCAAACCA 
AAACAAATTT GGTTGTTGTT AAGTTGTTTA GTATTTCATA CGCTTACGCT GGCCTCCTCA 
TCGTACATTG AATACGAGAA TATAACTTGG CATTATCTTG GAGCCAGCGC TATATTTTTG 
CTTTTTGTGC AAAATTTTCA AACACAGTTA TTTGCCTGCA GGCTGGACAC ATGCGTCAAT 
GATATGGCGC TGTATAAAGT TGTTTTGCGA AATTTGCTAA AATCACACAC GTCCCTATTG 
GTGATTGGTG GTTTGGGTAT TTTGTACCGT TTAGACGGTG CGTCGTCTTA TCTAACGCAG 
GAGCGAAATA AAGCATGGCT AAGTTTCATA TTTGGTGCAG GCCTGATTTT ATATACGCTA 
TGTTTACGTC AGCATAGTCT TGTGCAAACG CGTAAGGAGC TTGTGTTGTA CTTCTTCGCT 
GCGATGCTGA TCTATTGCTA TCGTGCCTTG AAGGGAGTGG TTTACTTCGT GTTCAACACA 
AACTGCTGTG ATAATATTGT TTTGAACATA TTTTGGCTGT TGGTCGTTAT TGCTGCATGC 
ATATCAACTG TGATATTACA TTCCAAACGC CGTATTAGTT ATCAAATTCA GCACACTTCG 
TTAGCGGGTT GCTTTAAAAT TCTACTAACT TTACTGCTGA CCGTTTCCTT GTTGCTGCAT 
AAGCCACACA ATGTTCTTCT AGTCGCTTAT TTAATATATA CGCTCTCTTT GTGCTATGAG 
TTGTGCGATA AATGCGAAAA GAACGCGCTA CGCAATTACA AATACAAGTA CTTATCGAAG 
CTGCTGTGCA CTATACTTGT GGGCAATATG TTTTACTTCT ATCAGGGTAA TTCGAATAGT 
TTTGCCACGA TCGATCTAAA TCCCGGCTAC ATTGGTCAAA CTACTTATAA TCCAGTAGTT 
GTCGGTATAC TTGTCACCTT GAATATGTAT TCAGCGCAAT TTATTGCATT TTTATACTTA 
ATTACGCATA TTTTGCAAGA AAGTGTTAAC AAAACAAACG GTTTGTCTGC AAATGAGCAT 
ATAAATTTCA TTATTTATTT CTACACCTTA AGCATATTTG TACCGGTTTT AATTTATTTC 
TGGTTACTGT TACTCTTTCG TTACCATCTG TTTATCTATT CAGTATTTGC GCCGAAAGCA 
TTTTATGAAT ACTACCGTGT ATTTGTACTT TTCTTTACAT ATTTAATGAC AACAATTAAC 
TTGTACATAT TCGATCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011190625.1
CDS	104..2884
Translation	MALQMEKSRPIRIFLICMIVSFIFGASIFLTGFFPINNYALDRVAAESKPKDLDGLSLTPPTQIYAQTILMLVDALRFDFASQNGIPFSYNNSCNRLKLRVNIPTVTMPRLKSLTTGTVSNFIDIILNIGHVEQLTDSLLHRLQERNETTVFAGDRTWISLFPKQFTRFTANSDSFFVNDFYEGDKNVTEVLSKELLNNDWMLLVLHYLGLDHIGHVEGSESAKIPLKLKEMDEVVLKLYNAKHFHRQLLLLTGDHGMRDGGGHGGSTTGEMYVPLFVFKENCTKSSASKSNEYNQIDMAPTLAILWSMEIPPMSIGCLIPELLHEFNMEDQLYAYYYNALHLVQKANSKFDEDFVNNNAFNKWFTTAKSAHKQFLLEKRNKNFENTFIFEHAKVHYLRLAREISNQLSDSLVQFDYGLITLGLMLTTFVTLNALLAFFTTTACVIIKGKAAWLCLLAFAACINKWCHYQRYFLSTGIMFTITILLAIMANTYLLINIVYILFRRLHMSCTLAQSSLPKPKQIWLLLSCLVFHTLTLASSSYIEYENITWHYLGASAIFLLFVQNFQTQLFACRLDTCVNDMALYKVVLRNLLKSHTSLLVIGGLGILYRLDGASSYLTQERNKAWLSFIFGAGLILYTLCLRQHSLVQTRKELVLYFFAAMLIYCYRALKGVVYFVFNTNCCDNIVLNIFWLLVVIAACISTVILHSKRRISYQIQHTSLAGCFKILLTLLLTVSLLLHKPHNVLLVAYLIYTLSLCYELCDKCEKNALRNYKYKYLSKLLCTILVGNMFYFYQGNSNSFATIDLNPGYIGQTTYNPVVVGILVTLNMYSAQFIAFLYLITHILQESVNKTNGLSANEHINFIIYFYTLSIFVPVLIYFWLLLLFRYHLFIYSVFAPKAFYEYYRVFVLFFTYLMTTINLYIFDQ

Target ORF information:

RefSeq Version	XM_011192323.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae GPI ethanolamine phosphate transferase 2 (Orc1_0), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192323.2

ORF Insert Sequence:

ATGGCATTAC AGATGGAAAA ATCAAGACCT ATTAGAATTT TTCTAATATG CATGATAGTT 
TCGTTTATTT TTGGCGCTTC AATTTTTCTA ACTGGTTTCT TTCCCATAAA CAATTATGCT 
TTGGATCGTG TTGCCGCTGA AAGCAAACCA AAAGATTTGG ATGGTTTGAG TTTGACACCG 
CCAACTCAAA TTTACGCACA AACCATACTT ATGCTGGTGG ATGCGCTACG TTTCGATTTT 
GCCAGTCAAA ATGGAATTCC TTTCTCCTAC AACAACTCAT GTAATCGTCT CAAGTTGCGT 
GTTAACATAC CCACTGTTAC CATGCCTCGT CTTAAAAGCT TAACCACCGG CACGGTTTCA 
AATTTTATTG ATATTATCCT AAATATTGGT CATGTTGAAC AACTTACAGA CTCTTTGTTA 
CACCGCTTAC AAGAACGTAA CGAAACTACG GTTTTTGCTG GTGACCGTAC TTGGATAAGT 
CTATTTCCAA AACAATTTAC ACGCTTTACC GCTAACTCAG ATTCGTTTTT TGTGAACGAT 
TTTTATGAGG GTGACAAAAA TGTGACGGAA GTATTATCTA AAGAATTGCT GAATAATGAC 
TGGATGTTGT TGGTGCTACA TTATTTGGGT TTAGATCATA TTGGACATGT TGAAGGTAGT 
GAAAGTGCCA AAATCCCGCT TAAATTAAAA GAAATGGATG AAGTTGTGCT GAAGCTATAC 
AATGCAAAGC ATTTTCACAG ACAGCTGCTT TTATTAACTG GTGACCATGG TATGCGTGAT 
GGTGGCGGTC ATGGTGGGAG TACAACGGGT GAGATGTATG TGCCATTATT TGTTTTCAAA 
GAAAACTGCA CCAAAAGCAG TGCTAGTAAA TCTAACGAGT ATAATCAAAT TGATATGGCG 
CCCACATTAG CCATACTCTG GTCCATGGAA ATTCCGCCGA TGTCAATTGG CTGTTTAATA 
CCCGAGCTCT TGCATGAGTT TAACATGGAG GACCAACTAT ACGCTTATTA TTATAATGCG 
CTACATTTAG TGCAAAAAGC TAACAGTAAA TTTGATGAAG ATTTTGTTAA TAACAATGCA 
TTCAACAAAT GGTTTACCAC AGCCAAATCG GCGCATAAGC AGTTTCTTTT AGAAAAACGC 
AATAAAAACT TTGAAAATAC TTTTATTTTC GAGCACGCTA AAGTGCATTA CTTACGTTTG 
GCACGCGAAA TTTCAAACCA ACTATCCGAC TCATTAGTGC AATTCGATTA CGGGCTGATC 
ACACTGGGGC TTATGCTAAC CACATTTGTC ACACTCAACG CACTTTTGGC TTTTTTCACA 
ACCACTGCAT GTGTTATTAT CAAAGGCAAG GCAGCATGGC TGTGTCTATT AGCTTTCGCC 
GCATGCATAA ATAAATGGTG CCACTACCAG CGTTACTTTT TGTCTACTGG CATAATGTTT 
ACCATTACGA TATTGTTGGC CATAATGGCT AACACTTATT TGCTTATAAA TATAGTGTAT 
ATATTATTTC GCCGATTACA TATGAGCTGT ACACTAGCAC AATCCTCACT GCCCAAACCA 
AAACAAATTT GGTTGTTGTT AAGTTGTTTA GTATTTCATA CGCTTACGCT GGCCTCCTCA 
TCGTACATTG AATACGAGAA TATAACTTGG CATTATCTTG GAGCCAGCGC TATATTTTTG 
CTTTTTGTGC AAAATTTTCA AACACAGTTA TTTGCCTGCA GGCTGGACAC ATGCGTCAAT 
GATATGGCGC TGTATAAAGT TGTTTTGCGA AATTTGCTAA AATCACACAC GTCCCTATTG 
GTGATTGGTG GTTTGGGTAT TTTGTACCGT TTAGACGGTG CGTCGTCTTA TCTAACGCAG 
GAGCGAAATA AAGCATGGCT AAGTTTCATA TTTGGTGCAG GCCTGATTTT ATATACGCTA 
TGTTTACGTC AGCATAGTCT TGTGCAAACG CGTAAGGAGC TTGTGTTGTA CTTCTTCGCT 
GCGATGCTGA TCTATTGCTA TCGTGCCTTG AAGGGAGTGG TTTACTTCGT GTTCAACACA 
AACTGCTGTG ATAATATTGT TTTGAACATA TTTTGGCTGT TGGTCGTTAT TGCTGCATGC 
ATATCAACTG TGATATTACA TTCCAAACGC CGTATTAGTT ATCAAATTCA GCACACTTCG 
TTAGCGGGTT GCTTTAAAAT TCTACTAACT TTACTGCTGA CCGTTTCCTT GTTGCTGCAT 
AAGCCACACA ATGTTCTTCT AGTCGCTTAT TTAATATATA CGCTCTCTTT GTGCTATGAG 
TTGTGCGATA AATGCGAAAA GAACGCGCTA CGCAATTACA AATACAAGTA CTTATCGAAG 
CTGCTGTGCA CTATACTTGT GGGCAATATG TTTTACTTCT ATCAGGGTAA TTCGAATAGT 
TTTGCCACGA TCGATCTAAA TCCCGGCTAC ATTGGTCAAA CTACTTATAA TCCAGTAGTT 
GTCGGTATAC TTGTCACCTT GAATATGTAT TCAGCGCAAT TTATTGCATT TTTATACTTA 
ATTACGCATA TTTTGCAAGA AAGTGTTAAC AAAACAAACG GTTTGTCTGC AAATGAGCAT 
ATAAATTTCA TTATTTATTT CTACACCTTA AGCATATTTG TACCGGTTTT AATTTATTTC 
TGGTTACTGT TACTCTTTCG TTACCATCTG TTTATCTATT CAGTATTTGC GCCGAAAGCA 
TTTTATGAAT ACTACCGTGT ATTTGTACTT TTCTTTACAT ATTTAATGAC AACAATTAAC 
TTGTACATAT TCGATCAATA A