LOC105215878 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105215878
Entrez Gene ID	105215878
Full Name	zinc finger protein 45
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011190057.1	XP_011188359.1	uncharacterized protein LOC105215878
XM_011190057.2	XP_011188359.1	uncharacterized protein LOC105215878

Pathways from BioSystems
Gene Ontology

The following LOC105215878 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105215878 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa202722	XM_011190057.2 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105215878 (LOC105215878), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OBa202722	XM_011190057.1	Bactrocera cucurbitae uncharacterized LOC105215878 (LOC105215878), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105215878 ( XM_011190057.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011188359.1 Bactrocera cucurbitae uncharacterized LOC105215878 (LOC105215878), mRNA.

Price & Availability↑

CloneID	OBa202722
Clone ID Related Accession (Same CDS sequence)	XM_011190057.1 , XM_011190057.2
Accession Version	XM_011190057.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105215878
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863761.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATCA ACACACTGCT AGAGCATGTA CCCATAAAAC TGGAAGCGCC GGATAATGGC 
GACGAGGGAG ACGCAGAAAA CAATAGCAAA CAGAAAAACA AAATTGTGAA AATGAATGGT 
AAGAATGTAG TCGTGGATGA AGATGTTAGC GGTGAAGATG ATGAAGAGGA CGACGAAGAC 
ACTGACGAGG ATGATTCTGA AACGGATCTA CTGGAGGAGG ATGATGATGC GACATTAGTA 
CCGATTAGCA GCGATGAAGA AGCAGAGCTG GAAGCCCAAC GTATACGATT CCATGAAATA 
ATAGGGAAAA ACTTGCATTT ACCAACTGTT TCCAAAAACG ATGCACTTAA AGGTGGTAAA 
AAGACAAAAT CTGCGAAGAA ACTACGTGGT CGCAGTGTTT CGAGTTGTGC TAGTGTCAGC 
AGTAAAACTT CCGTAGCTTC TTGCTCATCT GCCTCATCAG CTGCAAACAG CAAACGTTCG 
AAGCAGAGTG ATGTACCAAA GCAAAAACAA AAGTCAGATT CTAAGGTTAA AAAGACAGTT 
GCACGGAGAA ATGTGGAAAA AGGTACCAGT ACTGAATCAG AAGCCGAGCA TGATGATATT 
GCTGCAACAT ATAAACGTCC ACTTCGTAGT CGTGTTCCCT CCACTTGCTC TGTAGCATCA 
TCGCTAGTCA CGCCTTCATG TCAAACGGAA TCTTGTGGAA ATATTTATGT GTACGAGGAG 
CAGCAAAAAC TTATATTTAA TTGTAGCTTT TGCGACTTAC GTTATGGCGA TATGTCGTCG 
TTCGCAAAAC ATTTACATGA TGCACATAAA CTGTTTCAGG AGGATGAAGA TGTGACCACC 
ACAAAAGTAG CGCGTAAAAG TCCACGCACA CTAACGAAGC AACAGGCTGA AAATAAAGCG 
CAGGCGCAGG ATAACAAACT GAAGGTGGTA ATTAAGACGG AGATAGACCC ATACCCTGAA 
GAAATTGGTG ATGATAGCTC AAGTGCACAC TCAATGTCAC CAATTCCTGG TGATGCAACA 
CTGGAGTCAT GTGGAAATGT ATTTATTTTA AATGGCAAAA AGCTTTTCTT AATTTGTGGA 
CACTGTGAGT GCAAATATGC CACATTGGAT TTATTCCATA AACATTTGAG GCAACAACAT 
CAATTATTCT CCGGACCGGT AAAGGAAATA GATGTATTAC CCAAGTGTGA AATTAAGCTT 
GAGCCGTGCG ATGAAGTAGG GCAGCTAACG AAAATGAATG CAGCAGCTAT GGCAATTGAT 
AAACAGCGAA AAGCTAGCAT CGAAGCAATG TCAGCTTCGC CTGTGATGGT AGTGTTGCCA 
GTGTCACAAA ATGTGGACAA CAATGACATC TTGCCAGCAA CTGTTGCAGT GCAAGCGCCA 
GAAACGCCAC CCCCTGAAGA AGACAAGACA GTGGAAGCGA CAACAATAAA TACGTCCAAC 
AAACACGAAG CATTGTCTGA GAAAACAGTT ATGAGTGAAG CAGTCTGCGA AGTAGCAAAT 
GAAGAGGTGG ATGATAATGA AATACCGCCA AGGGTTGAGG AGCATGCGCA GTTGGAAACG 
ACGCAACAGG TGCCTGCTAC AGTAAAGTTT GTAAGTGAAA AAGCACACAA TTCTCTTGGA 
GAAACTATTG AAGATGTCGA ACACACAAAC AATGCGGAAG AACAGAAACC TGCAGCAAAA 
CGCAAAAGAA AGCGCCGATC GGTTTTTTCG AAACGTTCGC CGCCAAAGAA ACGAGCTGCA 
ACAACAAAAG CTGCAAATGC ACAACAAACT GAAAATAATG ACGAAAGCAA CACTGATGGC 
GCAATTGCAT TCCCTCCAGC CACTATATCT GTTGCAGAAC CCGCTAAAAT GGTTAATGAC 
GTCCACTTGG CCCCCATTGC CGAAGAAACA CCGATGGAAA CAGATTTCAC CGAAACGGAT 
GTCAAAACTT TGACTAATTT AGTGCAAGAT GTTGTTTCAG ATATAGCTGC CCCTATAAAC 
GCTAATGAAA TTAAAAAACA AAAAGCTAAA GAAAAGAAAG AAAGCGCAGA AAAAATAAAA 
ATCCACGCAA AGCCGACTGC CAAGCGAACT CGCAAAAATA AAAATCTTAC CGTTGAAAAC 
GAAGAATCAG TTTTACCGTC TAAAAGTGCT ACCGCCTCCA AATCTTCGTC GCCTGAATTC 
TCAGGCAAAT CTTCGCCTGA ACTCGATTTG GCATTGGATG CAACATTGGC GGCCAGTAAA 
CCAGTCGCAC ATCCCATGCG CACAACATGC TACTCCGAAA CTCATAAGCT ACGCTACGCT 
TGTAATCAGT GTCCAAAATC GTTCAGTAAG TCACCACGTC TGGCCGAACA CAAGCGTTTA 
CACACAGGCG AGAAGCCGTT CAGTTGCGAA GAATGTGGCA AAACTTTCCG CATTAAAAGA 
CGTCTAAGTG AGCATAAAAT GCGTCATCTC AAAGTGAAGG CATATAAGTG TGAAACCTGT 
GGCATGCCGG TGGCTACCAA ACAAGACCTC AGACTACATC AACGCCATCA CACCAATGAT 
AGGCGTTATA ATTGCCCTGA CTGTTCGAAA GCCTTTGTGC GCAGCTCCGA TTTGAAAATA 
CATAGACGTG TGCACACGGG TGAGAAGCCG TTCGTTTGTG AAATTTGCCA GAAAACATTT 
CGGGCCAACC AGAATCTCCT TGTGCATCGG CGTTCACACA TGGGCGAGAA GAATTATAAA 
TGCGATTATT GCGACAAACG CTTTATGCGT AATATCGACC GCAAGGTGCA CCATCGCACG 
CATACAGGTG AAAGACCATT TAAATGCGAA ATTTGTGGAC GGTGTTACTC TTCGCGTGCT 
CATGTGCGCG CACACATACA ACGTGAACAT GTCGAAAATG GTGGTGACAC AAAACCCGAA 
CGCAAGAAAG CCGAACGTAA GACAAAAGTA ACTGCAACGG ACGAAATTGT GAAGGAACAA 
CAGTTAATTG ATGAAATACA AGAACAAATA TTGCAGTGTT TAAAAACCGA CGATTTAGAT 
GATGAGGAGT TGCCAGCCAC AGCAACAGCA ACAACGACTA TTGTGAAAGC TGCTGAAATA 
AAAAAGCAGA AACGGCGAAA ATCAACACCC CGAACATCGC CGGCACTAAA GAACGGCGTT 
GACAACGATA TGCCCGCGCA TACACCCCAT CAGGCACAAA CGATGGGCGT GCAACAGGTA 
TCCGTAATGG TGTCAATGCA AGTGCAGGAC AATGAACCGA TGCCTGAGAA AGAGAACGCC 
TCGACGCTGG TGGGTGGGGT CTCAGCACCA CTAAGCAAGA GCACAAAGAG TGAGCGTAAA 
ATATCGAGTT ATTTCACTGT GTTGGGACAA AAAACAGAAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011188359.1
CDS	195..3539
Translation	MMINTLLEHVPIKLEAPDNGDEGDAENNSKQKNKIVKMNGKNVVVDEDVSGEDDEEDDEDTDEDDSETDLLEEDDDATLVPISSDEEAELEAQRIRFHEIIGKNLHLPTVSKNDALKGGKKTKSAKKLRGRSVSSCASVSSKTSVASCSSASSAANSKRSKQSDVPKQKQKSDSKVKKTVARRNVEKGTSTESEAEHDDIAATYKRPLRSRVPSTCSVASSLVTPSCQTESCGNIYVYEEQQKLIFNCSFCDLRYGDMSSFAKHLHDAHKLFQEDEDVTTTKVARKSPRTLTKQQAENKAQAQDNKLKVVIKTEIDPYPEEIGDDSSSAHSMSPIPGDATLESCGNVFILNGKKLFLICGHCECKYATLDLFHKHLRQQHQLFSGPVKEIDVLPKCEIKLEPCDEVGQLTKMNAAAMAIDKQRKASIEAMSASPVMVVLPVSQNVDNNDILPATVAVQAPETPPPEEDKTVEATTINTSNKHEALSEKTVMSEAVCEVANEEVDDNEIPPRVEEHAQLETTQQVPATVKFVSEKAHNSLGETIEDVEHTNNAEEQKPAAKRKRKRRSVFSKRSPPKKRAATTKAANAQQTENNDESNTDGAIAFPPATISVAEPAKMVNDVHLAPIAEETPMETDFTETDVKTLTNLVQDVVSDIAAPINANEIKKQKAKEKKESAEKIKIHAKPTAKRTRKNKNLTVENEESVLPSKSATASKSSSPEFSGKSSPELDLALDATLAASKPVAHPMRTTCYSETHKLRYACNQCPKSFSKSPRLAEHKRLHTGEKPFSCEECGKTFRIKRRLSEHKMRHLKVKAYKCETCGMPVATKQDLRLHQRHHTNDRRYNCPDCSKAFVRSSDLKIHRRVHTGEKPFVCEICQKTFRANQNLLVHRRSHMGEKNYKCDYCDKRFMRNIDRKVHHRTHTGERPFKCEICGRCYSSRAHVRAHIQREHVENGGDTKPERKKAERKTKVTATDEIVKEQQLIDEIQEQILQCLKTDDLDDEELPATATATTTIVKAAEIKKQKRRKSTPRTSPALKNGVDNDMPAHTPHQAQTMGVQQVSVMVSMQVQDNEPMPEKENASTLVGGVSAPLSKSTKSERKISSYFTVLGQKTEI

Target ORF information:

RefSeq Version	XM_011190057.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105215878 (LOC105215878), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011190057.1

ORF Insert Sequence:

ATGATGATCA ACACACTGCT AGAGCATGTA CCCATAAAAC TGGAAGCGCC GGATAATGGC 
GACGAGGGAG ACGCAGAAAA CAATAGCAAA CAGAAAAACA AAATTGTGAA AATGAATGGT 
AAGAATGTAG TCGTGGATGA AGATGTTAGC GGTGAAGATG ATGAAGAGGA CGACGAAGAC 
ACTGACGAGG ATGATTCTGA AACGGATCTA CTGGAGGAGG ATGATGATGC GACATTAGTA 
CCGATTAGCA GCGATGAAGA AGCAGAGCTG GAAGCCCAAC GTATACGATT CCATGAAATA 
ATAGGGAAAA ACTTGCATTT ACCAACTGTT TCCAAAAACG ATGCACTTAA AGGTGGTAAA 
AAGACAAAAT CTGCGAAGAA ACTACGTGGT CGCAGTGTTT CGAGTTGTGC TAGTGTCAGC 
AGTAAAACTT CCGTAGCTTC TTGCTCATCT GCCTCATCAG CTGCAAACAG CAAACGTTCG 
AAGCAGAGTG ATGTACCAAA GCAAAAACAA AAGTCAGATT CTAAGGTTAA AAAGACAGTT 
GCACGGAGAA ATGTGGAAAA AGGTACCAGT ACTGAATCAG AAGCCGAGCA TGATGATATT 
GCTGCAACAT ATAAACGTCC ACTTCGTAGT CGTGTTCCCT CCACTTGCTC TGTAGCATCA 
TCGCTAGTCA CGCCTTCATG TCAAACGGAA TCTTGTGGAA ATATTTATGT GTACGAGGAG 
CAGCAAAAAC TTATATTTAA TTGTAGCTTT TGCGACTTAC GTTATGGCGA TATGTCGTCG 
TTCGCAAAAC ATTTACATGA TGCACATAAA CTGTTTCAGG AGGATGAAGA TGTGACCACC 
ACAAAAGTAG CGCGTAAAAG TCCACGCACA CTAACGAAGC AACAGGCTGA AAATAAAGCG 
CAGGCGCAGG ATAACAAACT GAAGGTGGTA ATTAAGACGG AGATAGACCC ATACCCTGAA 
GAAATTGGTG ATGATAGCTC AAGTGCACAC TCAATGTCAC CAATTCCTGG TGATGCAACA 
CTGGAGTCAT GTGGAAATGT ATTTATTTTA AATGGCAAAA AGCTTTTCTT AATTTGTGGA 
CACTGTGAGT GCAAATATGC CACATTGGAT TTATTCCATA AACATTTGAG GCAACAACAT 
CAATTATTCT CCGGACCGGT AAAGGAAATA GATGTATTAC CCAAGTGTGA AATTAAGCTT 
GAGCCGTGCG ATGAAGTAGG GCAGCTAACG AAAATGAATG CAGCAGCTAT GGCAATTGAT 
AAACAGCGAA AAGCTAGCAT CGAAGCAATG TCAGCTTCGC CTGTGATGGT AGTGTTGCCA 
GTGTCACAAA ATGTGGACAA CAATGACATC TTGCCAGCAA CTGTTGCAGT GCAAGCGCCA 
GAAACGCCAC CCCCTGAAGA AGACAAGACA GTGGAAGCGA CAACAATAAA TACGTCCAAC 
AAACACGAAG CATTGTCTGA GAAAACAGTT ATGAGTGAAG CAGTCTGCGA AGTAGCAAAT 
GAAGAGGTGG ATGATAATGA AATACCGCCA AGGGTTGAGG AGCATGCGCA GTTGGAAACG 
ACGCAACAGG TGCCTGCTAC AGTAAAGTTT GTAAGTGAAA AAGCACACAA TTCTCTTGGA 
GAAACTATTG AAGATGTCGA ACACACAAAC AATGCGGAAG AACAGAAACC TGCAGCAAAA 
CGCAAAAGAA AGCGCCGATC GGTTTTTTCG AAACGTTCGC CGCCAAAGAA ACGAGCTGCA 
ACAACAAAAG CTGCAAATGC ACAACAAACT GAAAATAATG ACGAAAGCAA CACTGATGGC 
GCAATTGCAT TCCCTCCAGC CACTATATCT GTTGCAGAAC CCGCTAAAAT GGTTAATGAC 
GTCCACTTGG CCCCCATTGC CGAAGAAACA CCGATGGAAA CAGATTTCAC CGAAACGGAT 
GTCAAAACTT TGACTAATTT AGTGCAAGAT GTTGTTTCAG ATATAGCTGC CCCTATAAAC 
GCTAATGAAA TTAAAAAACA AAAAGCTAAA GAAAAGAAAG AAAGCGCAGA AAAAATAAAA 
ATCCACGCAA AGCCGACTGC CAAGCGAACT CGCAAAAATA AAAATCTTAC CGTTGAAAAC 
GAAGAATCAG TTTTACCGTC TAAAAGTGCT ACCGCCTCCA AATCTTCGTC GCCTGAATTC 
TCAGGCAAAT CTTCGCCTGA ACTCGATTTG GCATTGGATG CAACATTGGC GGCCAGTAAA 
CCAGTCGCAC ATCCCATGCG CACAACATGC TACTCCGAAA CTCATAAGCT ACGCTACGCT 
TGTAATCAGT GTCCAAAATC GTTCAGTAAG TCACCACGTC TGGCCGAACA CAAGCGTTTA 
CACACAGGCG AGAAGCCGTT CAGTTGCGAA GAATGTGGCA AAACTTTCCG CATTAAAAGA 
CGTCTAAGTG AGCATAAAAT GCGTCATCTC AAAGTGAAGG CATATAAGTG TGAAACCTGT 
GGCATGCCGG TGGCTACCAA ACAAGACCTC AGACTACATC AACGCCATCA CACCAATGAT 
AGGCGTTATA ATTGCCCTGA CTGTTCGAAA GCCTTTGTGC GCAGCTCCGA TTTGAAAATA 
CATAGACGTG TGCACACGGG TGAGAAGCCG TTCGTTTGTG AAATTTGCCA GAAAACATTT 
CGGGCCAACC AGAATCTCCT TGTGCATCGG CGTTCACACA TGGGCGAGAA GAATTATAAA 
TGCGATTATT GCGACAAACG CTTTATGCGT AATATCGACC GCAAGGTGCA CCATCGCACG 
CATACAGGTG AAAGACCATT TAAATGCGAA ATTTGTGGAC GGTGTTACTC TTCGCGTGCT 
CATGTGCGCG CACACATACA ACGTGAACAT GTCGAAAATG GTGGTGACAC AAAACCCGAA 
CGCAAGAAAG CCGAACGTAA GACAAAAGTA ACTGCAACGG ACGAAATTGT GAAGGAACAA 
CAGTTAATTG ATGAAATACA AGAACAAATA TTGCAGTGTT TAAAAACCGA CGATTTAGAT 
GATGAGGAGT TGCCAGCCAC AGCAACAGCA ACAACGACTA TTGTGAAAGC TGCTGAAATA 
AAAAAGCAGA AACGGCGAAA ATCAACACCC CGAACATCGC CGGCACTAAA GAACGGCGTT 
GACAACGATA TGCCCGCGCA TACACCCCAT CAGGCACAAA CGATGGGCGT GCAACAGGTA 
TCCGTAATGG TGTCAATGCA AGTGCAGGAC AATGAACCGA TGCCTGAGAA AGAGAACGCC 
TCGACGCTGG TGGGTGGGGT CTCAGCACCA CTAAGCAAGA GCACAAAGAG TGAGCGTAAA 
ATATCGAGTT ATTTCACTGT GTTGGGACAA AAAACAGAAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105215878 ( XM_011190057.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011188359.1 Zeugodacus cucurbitae uncharacterized LOC105215878 (LOC105215878), mRNA.

Price & Availability↑

CloneID	OBa202722
Clone ID Related Accession (Same CDS sequence)	XM_011190057.1 , XM_011190057.2
Accession Version	XM_011190057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105215878
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863761.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011190057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATCA ACACACTGCT AGAGCATGTA CCCATAAAAC TGGAAGCGCC GGATAATGGC 
GACGAGGGAG ACGCAGAAAA CAATAGCAAA CAGAAAAACA AAATTGTGAA AATGAATGGT 
AAGAATGTAG TCGTGGATGA AGATGTTAGC GGTGAAGATG ATGAAGAGGA CGACGAAGAC 
ACTGACGAGG ATGATTCTGA AACGGATCTA CTGGAGGAGG ATGATGATGC GACATTAGTA 
CCGATTAGCA GCGATGAAGA AGCAGAGCTG GAAGCCCAAC GTATACGATT CCATGAAATA 
ATAGGGAAAA ACTTGCATTT ACCAACTGTT TCCAAAAACG ATGCACTTAA AGGTGGTAAA 
AAGACAAAAT CTGCGAAGAA ACTACGTGGT CGCAGTGTTT CGAGTTGTGC TAGTGTCAGC 
AGTAAAACTT CCGTAGCTTC TTGCTCATCT GCCTCATCAG CTGCAAACAG CAAACGTTCG 
AAGCAGAGTG ATGTACCAAA GCAAAAACAA AAGTCAGATT CTAAGGTTAA AAAGACAGTT 
GCACGGAGAA ATGTGGAAAA AGGTACCAGT ACTGAATCAG AAGCCGAGCA TGATGATATT 
GCTGCAACAT ATAAACGTCC ACTTCGTAGT CGTGTTCCCT CCACTTGCTC TGTAGCATCA 
TCGCTAGTCA CGCCTTCATG TCAAACGGAA TCTTGTGGAA ATATTTATGT GTACGAGGAG 
CAGCAAAAAC TTATATTTAA TTGTAGCTTT TGCGACTTAC GTTATGGCGA TATGTCGTCG 
TTCGCAAAAC ATTTACATGA TGCACATAAA CTGTTTCAGG AGGATGAAGA TGTGACCACC 
ACAAAAGTAG CGCGTAAAAG TCCACGCACA CTAACGAAGC AACAGGCTGA AAATAAAGCG 
CAGGCGCAGG ATAACAAACT GAAGGTGGTA ATTAAGACGG AGATAGACCC ATACCCTGAA 
GAAATTGGTG ATGATAGCTC AAGTGCACAC TCAATGTCAC CAATTCCTGG TGATGCAACA 
CTGGAGTCAT GTGGAAATGT ATTTATTTTA AATGGCAAAA AGCTTTTCTT AATTTGTGGA 
CACTGTGAGT GCAAATATGC CACATTGGAT TTATTCCATA AACATTTGAG GCAACAACAT 
CAATTATTCT CCGGACCGGT AAAGGAAATA GATGTATTAC CCAAGTGTGA AATTAAGCTT 
GAGCCGTGCG ATGAAGTAGG GCAGCTAACG AAAATGAATG CAGCAGCTAT GGCAATTGAT 
AAACAGCGAA AAGCTAGCAT CGAAGCAATG TCAGCTTCGC CTGTGATGGT AGTGTTGCCA 
GTGTCACAAA ATGTGGACAA CAATGACATC TTGCCAGCAA CTGTTGCAGT GCAAGCGCCA 
GAAACGCCAC CCCCTGAAGA AGACAAGACA GTGGAAGCGA CAACAATAAA TACGTCCAAC 
AAACACGAAG CATTGTCTGA GAAAACAGTT ATGAGTGAAG CAGTCTGCGA AGTAGCAAAT 
GAAGAGGTGG ATGATAATGA AATACCGCCA AGGGTTGAGG AGCATGCGCA GTTGGAAACG 
ACGCAACAGG TGCCTGCTAC AGTAAAGTTT GTAAGTGAAA AAGCACACAA TTCTCTTGGA 
GAAACTATTG AAGATGTCGA ACACACAAAC AATGCGGAAG AACAGAAACC TGCAGCAAAA 
CGCAAAAGAA AGCGCCGATC GGTTTTTTCG AAACGTTCGC CGCCAAAGAA ACGAGCTGCA 
ACAACAAAAG CTGCAAATGC ACAACAAACT GAAAATAATG ACGAAAGCAA CACTGATGGC 
GCAATTGCAT TCCCTCCAGC CACTATATCT GTTGCAGAAC CCGCTAAAAT GGTTAATGAC 
GTCCACTTGG CCCCCATTGC CGAAGAAACA CCGATGGAAA CAGATTTCAC CGAAACGGAT 
GTCAAAACTT TGACTAATTT AGTGCAAGAT GTTGTTTCAG ATATAGCTGC CCCTATAAAC 
GCTAATGAAA TTAAAAAACA AAAAGCTAAA GAAAAGAAAG AAAGCGCAGA AAAAATAAAA 
ATCCACGCAA AGCCGACTGC CAAGCGAACT CGCAAAAATA AAAATCTTAC CGTTGAAAAC 
GAAGAATCAG TTTTACCGTC TAAAAGTGCT ACCGCCTCCA AATCTTCGTC GCCTGAATTC 
TCAGGCAAAT CTTCGCCTGA ACTCGATTTG GCATTGGATG CAACATTGGC GGCCAGTAAA 
CCAGTCGCAC ATCCCATGCG CACAACATGC TACTCCGAAA CTCATAAGCT ACGCTACGCT 
TGTAATCAGT GTCCAAAATC GTTCAGTAAG TCACCACGTC TGGCCGAACA CAAGCGTTTA 
CACACAGGCG AGAAGCCGTT CAGTTGCGAA GAATGTGGCA AAACTTTCCG CATTAAAAGA 
CGTCTAAGTG AGCATAAAAT GCGTCATCTC AAAGTGAAGG CATATAAGTG TGAAACCTGT 
GGCATGCCGG TGGCTACCAA ACAAGACCTC AGACTACATC AACGCCATCA CACCAATGAT 
AGGCGTTATA ATTGCCCTGA CTGTTCGAAA GCCTTTGTGC GCAGCTCCGA TTTGAAAATA 
CATAGACGTG TGCACACGGG TGAGAAGCCG TTCGTTTGTG AAATTTGCCA GAAAACATTT 
CGGGCCAACC AGAATCTCCT TGTGCATCGG CGTTCACACA TGGGCGAGAA GAATTATAAA 
TGCGATTATT GCGACAAACG CTTTATGCGT AATATCGACC GCAAGGTGCA CCATCGCACG 
CATACAGGTG AAAGACCATT TAAATGCGAA ATTTGTGGAC GGTGTTACTC TTCGCGTGCT 
CATGTGCGCG CACACATACA ACGTGAACAT GTCGAAAATG GTGGTGACAC AAAACCCGAA 
CGCAAGAAAG CCGAACGTAA GACAAAAGTA ACTGCAACGG ACGAAATTGT GAAGGAACAA 
CAGTTAATTG ATGAAATACA AGAACAAATA TTGCAGTGTT TAAAAACCGA CGATTTAGAT 
GATGAGGAGT TGCCAGCCAC AGCAACAGCA ACAACGACTA TTGTGAAAGC TGCTGAAATA 
AAAAAGCAGA AACGGCGAAA ATCAACACCC CGAACATCGC CGGCACTAAA GAACGGCGTT 
GACAACGATA TGCCCGCGCA TACACCCCAT CAGGCACAAA CGATGGGCGT GCAACAGGTA 
TCCGTAATGG TGTCAATGCA AGTGCAGGAC AATGAACCGA TGCCTGAGAA AGAGAACGCC 
TCGACGCTGG TGGGTGGGGT CTCAGCACCA CTAAGCAAGA GCACAAAGAG TGAGCGTAAA 
ATATCGAGTT ATTTCACTGT GTTGGGACAA AAAACAGAAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011188359.1
CDS	481..3825
Translation	MMINTLLEHVPIKLEAPDNGDEGDAENNSKQKNKIVKMNGKNVVVDEDVSGEDDEEDDEDTDEDDSETDLLEEDDDATLVPISSDEEAELEAQRIRFHEIIGKNLHLPTVSKNDALKGGKKTKSAKKLRGRSVSSCASVSSKTSVASCSSASSAANSKRSKQSDVPKQKQKSDSKVKKTVARRNVEKGTSTESEAEHDDIAATYKRPLRSRVPSTCSVASSLVTPSCQTESCGNIYVYEEQQKLIFNCSFCDLRYGDMSSFAKHLHDAHKLFQEDEDVTTTKVARKSPRTLTKQQAENKAQAQDNKLKVVIKTEIDPYPEEIGDDSSSAHSMSPIPGDATLESCGNVFILNGKKLFLICGHCECKYATLDLFHKHLRQQHQLFSGPVKEIDVLPKCEIKLEPCDEVGQLTKMNAAAMAIDKQRKASIEAMSASPVMVVLPVSQNVDNNDILPATVAVQAPETPPPEEDKTVEATTINTSNKHEALSEKTVMSEAVCEVANEEVDDNEIPPRVEEHAQLETTQQVPATVKFVSEKAHNSLGETIEDVEHTNNAEEQKPAAKRKRKRRSVFSKRSPPKKRAATTKAANAQQTENNDESNTDGAIAFPPATISVAEPAKMVNDVHLAPIAEETPMETDFTETDVKTLTNLVQDVVSDIAAPINANEIKKQKAKEKKESAEKIKIHAKPTAKRTRKNKNLTVENEESVLPSKSATASKSSSPEFSGKSSPELDLALDATLAASKPVAHPMRTTCYSETHKLRYACNQCPKSFSKSPRLAEHKRLHTGEKPFSCEECGKTFRIKRRLSEHKMRHLKVKAYKCETCGMPVATKQDLRLHQRHHTNDRRYNCPDCSKAFVRSSDLKIHRRVHTGEKPFVCEICQKTFRANQNLLVHRRSHMGEKNYKCDYCDKRFMRNIDRKVHHRTHTGERPFKCEICGRCYSSRAHVRAHIQREHVENGGDTKPERKKAERKTKVTATDEIVKEQQLIDEIQEQILQCLKTDDLDDEELPATATATTTIVKAAEIKKQKRRKSTPRTSPALKNGVDNDMPAHTPHQAQTMGVQQVSVMVSMQVQDNEPMPEKENASTLVGGVSAPLSKSTKSERKISSYFTVLGQKTEI

Target ORF information:

RefSeq Version	XM_011190057.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105215878 (LOC105215878), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011190057.2

ORF Insert Sequence:

ATGATGATCA ACACACTGCT AGAGCATGTA CCCATAAAAC TGGAAGCGCC GGATAATGGC 
GACGAGGGAG ACGCAGAAAA CAATAGCAAA CAGAAAAACA AAATTGTGAA AATGAATGGT 
AAGAATGTAG TCGTGGATGA AGATGTTAGC GGTGAAGATG ATGAAGAGGA CGACGAAGAC 
ACTGACGAGG ATGATTCTGA AACGGATCTA CTGGAGGAGG ATGATGATGC GACATTAGTA 
CCGATTAGCA GCGATGAAGA AGCAGAGCTG GAAGCCCAAC GTATACGATT CCATGAAATA 
ATAGGGAAAA ACTTGCATTT ACCAACTGTT TCCAAAAACG ATGCACTTAA AGGTGGTAAA 
AAGACAAAAT CTGCGAAGAA ACTACGTGGT CGCAGTGTTT CGAGTTGTGC TAGTGTCAGC 
AGTAAAACTT CCGTAGCTTC TTGCTCATCT GCCTCATCAG CTGCAAACAG CAAACGTTCG 
AAGCAGAGTG ATGTACCAAA GCAAAAACAA AAGTCAGATT CTAAGGTTAA AAAGACAGTT 
GCACGGAGAA ATGTGGAAAA AGGTACCAGT ACTGAATCAG AAGCCGAGCA TGATGATATT 
GCTGCAACAT ATAAACGTCC ACTTCGTAGT CGTGTTCCCT CCACTTGCTC TGTAGCATCA 
TCGCTAGTCA CGCCTTCATG TCAAACGGAA TCTTGTGGAA ATATTTATGT GTACGAGGAG 
CAGCAAAAAC TTATATTTAA TTGTAGCTTT TGCGACTTAC GTTATGGCGA TATGTCGTCG 
TTCGCAAAAC ATTTACATGA TGCACATAAA CTGTTTCAGG AGGATGAAGA TGTGACCACC 
ACAAAAGTAG CGCGTAAAAG TCCACGCACA CTAACGAAGC AACAGGCTGA AAATAAAGCG 
CAGGCGCAGG ATAACAAACT GAAGGTGGTA ATTAAGACGG AGATAGACCC ATACCCTGAA 
GAAATTGGTG ATGATAGCTC AAGTGCACAC TCAATGTCAC CAATTCCTGG TGATGCAACA 
CTGGAGTCAT GTGGAAATGT ATTTATTTTA AATGGCAAAA AGCTTTTCTT AATTTGTGGA 
CACTGTGAGT GCAAATATGC CACATTGGAT TTATTCCATA AACATTTGAG GCAACAACAT 
CAATTATTCT CCGGACCGGT AAAGGAAATA GATGTATTAC CCAAGTGTGA AATTAAGCTT 
GAGCCGTGCG ATGAAGTAGG GCAGCTAACG AAAATGAATG CAGCAGCTAT GGCAATTGAT 
AAACAGCGAA AAGCTAGCAT CGAAGCAATG TCAGCTTCGC CTGTGATGGT AGTGTTGCCA 
GTGTCACAAA ATGTGGACAA CAATGACATC TTGCCAGCAA CTGTTGCAGT GCAAGCGCCA 
GAAACGCCAC CCCCTGAAGA AGACAAGACA GTGGAAGCGA CAACAATAAA TACGTCCAAC 
AAACACGAAG CATTGTCTGA GAAAACAGTT ATGAGTGAAG CAGTCTGCGA AGTAGCAAAT 
GAAGAGGTGG ATGATAATGA AATACCGCCA AGGGTTGAGG AGCATGCGCA GTTGGAAACG 
ACGCAACAGG TGCCTGCTAC AGTAAAGTTT GTAAGTGAAA AAGCACACAA TTCTCTTGGA 
GAAACTATTG AAGATGTCGA ACACACAAAC AATGCGGAAG AACAGAAACC TGCAGCAAAA 
CGCAAAAGAA AGCGCCGATC GGTTTTTTCG AAACGTTCGC CGCCAAAGAA ACGAGCTGCA 
ACAACAAAAG CTGCAAATGC ACAACAAACT GAAAATAATG ACGAAAGCAA CACTGATGGC 
GCAATTGCAT TCCCTCCAGC CACTATATCT GTTGCAGAAC CCGCTAAAAT GGTTAATGAC 
GTCCACTTGG CCCCCATTGC CGAAGAAACA CCGATGGAAA CAGATTTCAC CGAAACGGAT 
GTCAAAACTT TGACTAATTT AGTGCAAGAT GTTGTTTCAG ATATAGCTGC CCCTATAAAC 
GCTAATGAAA TTAAAAAACA AAAAGCTAAA GAAAAGAAAG AAAGCGCAGA AAAAATAAAA 
ATCCACGCAA AGCCGACTGC CAAGCGAACT CGCAAAAATA AAAATCTTAC CGTTGAAAAC 
GAAGAATCAG TTTTACCGTC TAAAAGTGCT ACCGCCTCCA AATCTTCGTC GCCTGAATTC 
TCAGGCAAAT CTTCGCCTGA ACTCGATTTG GCATTGGATG CAACATTGGC GGCCAGTAAA 
CCAGTCGCAC ATCCCATGCG CACAACATGC TACTCCGAAA CTCATAAGCT ACGCTACGCT 
TGTAATCAGT GTCCAAAATC GTTCAGTAAG TCACCACGTC TGGCCGAACA CAAGCGTTTA 
CACACAGGCG AGAAGCCGTT CAGTTGCGAA GAATGTGGCA AAACTTTCCG CATTAAAAGA 
CGTCTAAGTG AGCATAAAAT GCGTCATCTC AAAGTGAAGG CATATAAGTG TGAAACCTGT 
GGCATGCCGG TGGCTACCAA ACAAGACCTC AGACTACATC AACGCCATCA CACCAATGAT 
AGGCGTTATA ATTGCCCTGA CTGTTCGAAA GCCTTTGTGC GCAGCTCCGA TTTGAAAATA 
CATAGACGTG TGCACACGGG TGAGAAGCCG TTCGTTTGTG AAATTTGCCA GAAAACATTT 
CGGGCCAACC AGAATCTCCT TGTGCATCGG CGTTCACACA TGGGCGAGAA GAATTATAAA 
TGCGATTATT GCGACAAACG CTTTATGCGT AATATCGACC GCAAGGTGCA CCATCGCACG 
CATACAGGTG AAAGACCATT TAAATGCGAA ATTTGTGGAC GGTGTTACTC TTCGCGTGCT 
CATGTGCGCG CACACATACA ACGTGAACAT GTCGAAAATG GTGGTGACAC AAAACCCGAA 
CGCAAGAAAG CCGAACGTAA GACAAAAGTA ACTGCAACGG ACGAAATTGT GAAGGAACAA 
CAGTTAATTG ATGAAATACA AGAACAAATA TTGCAGTGTT TAAAAACCGA CGATTTAGAT 
GATGAGGAGT TGCCAGCCAC AGCAACAGCA ACAACGACTA TTGTGAAAGC TGCTGAAATA 
AAAAAGCAGA AACGGCGAAA ATCAACACCC CGAACATCGC CGGCACTAAA GAACGGCGTT 
GACAACGATA TGCCCGCGCA TACACCCCAT CAGGCACAAA CGATGGGCGT GCAACAGGTA 
TCCGTAATGG TGTCAATGCA AGTGCAGGAC AATGAACCGA TGCCTGAGAA AGAGAACGCC 
TCGACGCTGG TGGGTGGGGT CTCAGCACCA CTAAGCAAGA GCACAAAGAG TGAGCGTAAA 
ATATCGAGTT ATTTCACTGT GTTGGGACAA AAAACAGAAA TCTAA