LOC105215648 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105215648
Entrez Gene ID	105215648
Full Name	flocculation protein FLO11
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011189669.1	XP_011187971.1	flocculation protein FLO11
XM_029042520.1	XP_028898353.1	probable serine/threonine-protein kinase DDB_G0267686

Pathways from BioSystems
Gene Ontology

The following LOC105215648 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105215648 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa202443	XM_011189669.1 Latest version!	Bactrocera cucurbitae flocculation protein FLO11 (LOC105215648), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa256865	XM_029042520.1 Latest version!	Zeugodacus cucurbitae probable serine/threonine-protein kinase DDB_G0267686 (LOC105215648), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105215648 ( XM_011189669.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011187971.1 Bactrocera cucurbitae flocculation protein FLO11 (LOC105215648), mRNA.

Price & Availability↑

CloneID	OBa202443
Clone ID Related Accession (Same CDS sequence)	XM_011189669.1
Accession Version	XM_011189669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	flocculation protein FLO11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863756.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTGGCGTC AACTGATCGT GAGTGATCGA AAATTTAATA TTTATTTCAT TGCAGAAAAT 
ATAACGATAG TCGGCCTCCC GGACTATGTG AACAACAATC TCAAAATAGC ACTGTACGCA 
AAAGAAACAC AACGGTATCT GTGTTTCAAC GATCAGTGGA AACTAGTTGG AATGAAAGAA 
CTGCGCGACA CCTGCTACTT CAACGAAACC ATCGTACACG GTTATTTCGT GTTTCGTTCC 
GTAGTCGATC TGCAGCGACG TGTCGGCTTC ACGCATCGCG GTAAGGCGGT CGGGCCGAAG 
AAGAATGTCA ACGATGCCTG CTATCTGTTC ACGAAGATAC AGGCCGATCA GTTTTTCCAT 
CAGCACACCG ATTATTTTGC ACCGAAATCG AAGAGCACCA AAATCATCAG CGGCATTGGT 
CACTCACCGC CGCAAACAAC AACAACAGCC ACGCATACAA CATCAGGCGC CTCAGCGCGA 
CTGGCGTCAC AGCGCGCTGC ACTCTCGGCA GCTGCTACAA CGGCGACTGT CACAGCAACA 
GCGGCGCAAC GCAACAACAA CAGGCTATTG CGTAACAACA ACAACAAGAA TAAGCGAGTG 
AATGGCATGC GCAGCGGCAA CAATAATAGC CGGAGCAGTA ACAATAACCA AAATGCAGAG 
TACACTAATA ATGGCCATAA TAGCAATTTA AGCAGCAGCA GCAACAATAA TAATAACAAA 
AACAACACCA ACAATCAGAG CGTTAATAAT AACCATAATA ACAACAGTAA TTTAGTTAGC 
AATAGTCAGC AGCAGCAGCA GCAAGTAGAT TTAAGTCAAC AGAAGCAACA CAACAACAGC 
AATGGTCGCA CGCGCGAACA TGTGCGTCAG CAACAACAAC ATCAGCATCA AGTGCGGCAT 
CATCACAACG ATCCGAACTT GATAGCACGT CGCCAACAAC ACGAGCACAA ACAGAAGCGG 
CGACAGCATC AGCGCCAGCA GCAGCAGCGA CTGCGTAGCG GCAGAATAAC TAGTCCAGCA 
ACAACAACAG TAGCGCTAAA TGACGCGCAA GAACGCGCCA CAACGACGAA AGCGCTTTAC 
AGCACGGCGG CAACAGCTGC TGTGGCGCGC AGCAATCCGC AGCAGGAGAA AGGCACGCGT 
CATGAACCGC AAGCGGCGAC TGTTGCAAAT GTGGCGGCTA AGGCGGCAAC GGCAACTTCA 
ATAATTATGA AGTCAACAAC AACCATTGCC AATAAGCGTA AGGGACGGCG AAGAAAGGGG 
CGAAAGCAAC GAAAACAGCA GCAGCAACAA CAACCACAGC GCGCACAACA ACAAATGTCG 
CCGCTCGTCG CGCATGAACC CGCGACACCA ACGCAAACCA CTCGGCTGTG GAGCGATTCA 
CCAACGCCAC CACTTCCGTC GCTGCCAGCG CAGACGCAGG CCGATGTGCC GAGCAGCATA 
CCAACCGCAA CAACATACGG CGATTACAGC GGCGAATGGC AGTCCTCAGT GCCGACGTAC 
GCCGACGCGT CGACCGTGGA CACCGCGGCG TCTTCATCCT TAACACCAAC ACTGTCTTCG 
GACATACCTA CACAAACATA CACGGCCTCA ACAACCGATC TGATCGATGG TGCCTCGTCG 
CTCACGCCAA CAAGCGACTA CAACAACAAC ACTGAATTCA CAAGCGAAAC GCTGGCGTTT 
GCCACCGAAA TCGATGACAG TCGTATGACG CCGGAGTCTA CAATGTCGGC CGACGAACAA 
ATAAACAGTA TTACTTCCCA ACAACAACAC TTTACAACAC AAAATATGGC CACGCCAGTG 
TCGACTGCGC AATCCGTGGA GCCTACGTCA ACCACGTGGT CCGAGAGCAG TGAACACAGC 
TGGTCACCTA CCACTACAAC CAATGAAACC GATGATGGCG AGACACTGTT CGCCTCTCCG 
ACTGCAATGT TCGACGCGGA AAGCGTTGCA GCCACAGCAA TCTTCGCAGA GGACAACAAT 
GCTGCAGAGG AAACGCAAAC CGTTGCTGTG GACACCGATT CATTTGCAAC AATCAACACT 
ACCTCCAGTG AAATTGAAGC GGAAAGCAGC AGCAGCAATA GCAACACCAA CAACGACGGC 
TTCACTGAAG CGCTAGCGGA TGATGTTGCT GGCACGCAGA GCACAACAAC AGCACACCAC 
AGAGTCTTTA ATTTACCAGC TGAACGGGCG CATATCGGTC ACATGTGGAA GCAGCAGTCG 
GGCATTGCGG CGAGCATAGC CTCGCTGTTG CACAAACAAC TGCTGACGAC GAGCGCCAGC 
AGCGGCAACA AATACGCTTG GCGGCCGCAG CGCGCTGAAG AATACTCAGA TGAAATAATC 
GTGGCTACAG GACGGCCAGT CGCGACTGAA ACGGAAACGA CAACCGATAT TGCAGCGGGC 
GATCGTGCAA CATGGTGGCA ACACCATATG AATCACAGCG GCACGAACTT TTGGGACAAA 
GGCATTGCGG ATGAGGTGCT AAGCAGCGGC GACGATGCCG ATTTGGATTG GCTGAATCAC 
CACAACAAGC ACCGCAATGC AAGCAAGCGC CGGCATAACA ACCGCAAGGC CAACAACAGC 
CGAGATGTTG AAGCCGTGTC ACAGCAACCG TTGCAAGCGT TGTTGACGAG CGATTCGAGC 
CTTAGTTTTA GCAAAAAATA TTTAGCCGAA GGCAAATTAC AACGACAAAG TAGCAAAGCA 
GTAAGCGGCA ACAACAAATT GCTGGCATCA ACAACAACAG CGCATGAGTA CAGCAGCACA 
ATGGCGACAG CGCGTGCTAG TGGTGCAACA ACACACACGG AAGGTGTTAT TAATACCCCA 
ACAACAACAA CAGTGAGTGA TAAAACATTG ACAACAACGA CAACGACAGC ACAACGCCAT 
TATCCCCTGC AGCAATTGCT GGGCAATAAA ACACTCTTGC CGCTGCACAA TCAACCGCCG 
TTGTTGCATT TCGATGGCAA CAGCAATCAC TTGGCACACA CACATCCACC CACAGCAACC 
ACGCTCCTCA CATCAACTTC CGCTGCCGCC GCTGCGCCAA CTTCAACAAC ACTTGGCGGC 
GACGCGACAA CTGCCGCGGC AGCGTTGACC ACCAAAAAGT CGATACGCAT CTCGACGCAG 
AAATTGTTGG CGACACCCTT CCATCAGCTC ACCTATGTGC GCAACAACGC CGGCGACATC 
GATATCGACA ATATGGACAA CATCAGCGTC TATCCGGATC TGCTGGAGGA GTCGGGCGAA 
TTGGCGGATC AACATTCGAC GGAGCGCGCC GAACCCGATG CGACACGCAA TAGCCGACTG 
ACAATCGTTA GCGGTTATCT GCCCACTTCG GCCACGGCGA CGCTACCCGA ATCGATACGT 
CTGGCTAAGA TTAAGATTAA CAAGGAGAGA AAGCGCATGA TGAGCATGAG AAAAAGACAC 
ACCAGGCGTT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011187971.1
CDS	1..3438
Translation	MWRQLIVSDRKFNIYFIAENITIVGLPDYVNNNLKIALYAKETQRYLCFNDQWKLVGMKELRDTCYFNETIVHGYFVFRSVVDLQRRVGFTHRGKAVGPKKNVNDACYLFTKIQADQFFHQHTDYFAPKSKSTKIISGIGHSPPQTTTTATHTTSGASARLASQRAALSAAATTATVTATAAQRNNNRLLRNNNNKNKRVNGMRSGNNNSRSSNNNQNAEYTNNGHNSNLSSSSNNNNNKNNTNNQSVNNNHNNNSNLVSNSQQQQQQVDLSQQKQHNNSNGRTREHVRQQQQHQHQVRHHHNDPNLIARRQQHEHKQKRRQHQRQQQQRLRSGRITSPATTTVALNDAQERATTTKALYSTAATAAVARSNPQQEKGTRHEPQAATVANVAAKAATATSIIMKSTTTIANKRKGRRRKGRKQRKQQQQQQPQRAQQQMSPLVAHEPATPTQTTRLWSDSPTPPLPSLPAQTQADVPSSIPTATTYGDYSGEWQSSVPTYADASTVDTAASSSLTPTLSSDIPTQTYTASTTDLIDGASSLTPTSDYNNNTEFTSETLAFATEIDDSRMTPESTMSADEQINSITSQQQHFTTQNMATPVSTAQSVEPTSTTWSESSEHSWSPTTTTNETDDGETLFASPTAMFDAESVAATAIFAEDNNAAEETQTVAVDTDSFATINTTSSEIEAESSSSNSNTNNDGFTEALADDVAGTQSTTTAHHRVFNLPAERAHIGHMWKQQSGIAASIASLLHKQLLTTSASSGNKYAWRPQRAEEYSDEIIVATGRPVATETETTTDIAAGDRATWWQHHMNHSGTNFWDKGIADEVLSSGDDADLDWLNHHNKHRNASKRRHNNRKANNSRDVEAVSQQPLQALLTSDSSLSFSKKYLAEGKLQRQSSKAVSGNNKLLASTTTAHEYSSTMATARASGATTHTEGVINTPTTTTVSDKTLTTTTTTAQRHYPLQQLLGNKTLLPLHNQPPLLHFDGNSNHLAHTHPPTATTLLTSTSAAAAAPTSTTLGGDATTAAAALTTKKSIRISTQKLLATPFHQLTYVRNNAGDIDIDNMDNISVYPDLLEESGELADQHSTERAEPDATRNSRLTIVSGYLPTSATATLPESIRLAKIKINKERKRMMSMRKRHTRRYA

Target ORF information:

RefSeq Version	XM_011189669.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae flocculation protein FLO11 (LOC105215648), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011189669.1

ORF Insert Sequence:

ATGTGGCGTC AACTGATCGT GAGTGATCGA AAATTTAATA TTTATTTCAT TGCAGAAAAT 
ATAACGATAG TCGGCCTCCC GGACTATGTG AACAACAATC TCAAAATAGC ACTGTACGCA 
AAAGAAACAC AACGGTATCT GTGTTTCAAC GATCAGTGGA AACTAGTTGG AATGAAAGAA 
CTGCGCGACA CCTGCTACTT CAACGAAACC ATCGTACACG GTTATTTCGT GTTTCGTTCC 
GTAGTCGATC TGCAGCGACG TGTCGGCTTC ACGCATCGCG GTAAGGCGGT CGGGCCGAAG 
AAGAATGTCA ACGATGCCTG CTATCTGTTC ACGAAGATAC AGGCCGATCA GTTTTTCCAT 
CAGCACACCG ATTATTTTGC ACCGAAATCG AAGAGCACCA AAATCATCAG CGGCATTGGT 
CACTCACCGC CGCAAACAAC AACAACAGCC ACGCATACAA CATCAGGCGC CTCAGCGCGA 
CTGGCGTCAC AGCGCGCTGC ACTCTCGGCA GCTGCTACAA CGGCGACTGT CACAGCAACA 
GCGGCGCAAC GCAACAACAA CAGGCTATTG CGTAACAACA ACAACAAGAA TAAGCGAGTG 
AATGGCATGC GCAGCGGCAA CAATAATAGC CGGAGCAGTA ACAATAACCA AAATGCAGAG 
TACACTAATA ATGGCCATAA TAGCAATTTA AGCAGCAGCA GCAACAATAA TAATAACAAA 
AACAACACCA ACAATCAGAG CGTTAATAAT AACCATAATA ACAACAGTAA TTTAGTTAGC 
AATAGTCAGC AGCAGCAGCA GCAAGTAGAT TTAAGTCAAC AGAAGCAACA CAACAACAGC 
AATGGTCGCA CGCGCGAACA TGTGCGTCAG CAACAACAAC ATCAGCATCA AGTGCGGCAT 
CATCACAACG ATCCGAACTT GATAGCACGT CGCCAACAAC ACGAGCACAA ACAGAAGCGG 
CGACAGCATC AGCGCCAGCA GCAGCAGCGA CTGCGTAGCG GCAGAATAAC TAGTCCAGCA 
ACAACAACAG TAGCGCTAAA TGACGCGCAA GAACGCGCCA CAACGACGAA AGCGCTTTAC 
AGCACGGCGG CAACAGCTGC TGTGGCGCGC AGCAATCCGC AGCAGGAGAA AGGCACGCGT 
CATGAACCGC AAGCGGCGAC TGTTGCAAAT GTGGCGGCTA AGGCGGCAAC GGCAACTTCA 
ATAATTATGA AGTCAACAAC AACCATTGCC AATAAGCGTA AGGGACGGCG AAGAAAGGGG 
CGAAAGCAAC GAAAACAGCA GCAGCAACAA CAACCACAGC GCGCACAACA ACAAATGTCG 
CCGCTCGTCG CGCATGAACC CGCGACACCA ACGCAAACCA CTCGGCTGTG GAGCGATTCA 
CCAACGCCAC CACTTCCGTC GCTGCCAGCG CAGACGCAGG CCGATGTGCC GAGCAGCATA 
CCAACCGCAA CAACATACGG CGATTACAGC GGCGAATGGC AGTCCTCAGT GCCGACGTAC 
GCCGACGCGT CGACCGTGGA CACCGCGGCG TCTTCATCCT TAACACCAAC ACTGTCTTCG 
GACATACCTA CACAAACATA CACGGCCTCA ACAACCGATC TGATCGATGG TGCCTCGTCG 
CTCACGCCAA CAAGCGACTA CAACAACAAC ACTGAATTCA CAAGCGAAAC GCTGGCGTTT 
GCCACCGAAA TCGATGACAG TCGTATGACG CCGGAGTCTA CAATGTCGGC CGACGAACAA 
ATAAACAGTA TTACTTCCCA ACAACAACAC TTTACAACAC AAAATATGGC CACGCCAGTG 
TCGACTGCGC AATCCGTGGA GCCTACGTCA ACCACGTGGT CCGAGAGCAG TGAACACAGC 
TGGTCACCTA CCACTACAAC CAATGAAACC GATGATGGCG AGACACTGTT CGCCTCTCCG 
ACTGCAATGT TCGACGCGGA AAGCGTTGCA GCCACAGCAA TCTTCGCAGA GGACAACAAT 
GCTGCAGAGG AAACGCAAAC CGTTGCTGTG GACACCGATT CATTTGCAAC AATCAACACT 
ACCTCCAGTG AAATTGAAGC GGAAAGCAGC AGCAGCAATA GCAACACCAA CAACGACGGC 
TTCACTGAAG CGCTAGCGGA TGATGTTGCT GGCACGCAGA GCACAACAAC AGCACACCAC 
AGAGTCTTTA ATTTACCAGC TGAACGGGCG CATATCGGTC ACATGTGGAA GCAGCAGTCG 
GGCATTGCGG CGAGCATAGC CTCGCTGTTG CACAAACAAC TGCTGACGAC GAGCGCCAGC 
AGCGGCAACA AATACGCTTG GCGGCCGCAG CGCGCTGAAG AATACTCAGA TGAAATAATC 
GTGGCTACAG GACGGCCAGT CGCGACTGAA ACGGAAACGA CAACCGATAT TGCAGCGGGC 
GATCGTGCAA CATGGTGGCA ACACCATATG AATCACAGCG GCACGAACTT TTGGGACAAA 
GGCATTGCGG ATGAGGTGCT AAGCAGCGGC GACGATGCCG ATTTGGATTG GCTGAATCAC 
CACAACAAGC ACCGCAATGC AAGCAAGCGC CGGCATAACA ACCGCAAGGC CAACAACAGC 
CGAGATGTTG AAGCCGTGTC ACAGCAACCG TTGCAAGCGT TGTTGACGAG CGATTCGAGC 
CTTAGTTTTA GCAAAAAATA TTTAGCCGAA GGCAAATTAC AACGACAAAG TAGCAAAGCA 
GTAAGCGGCA ACAACAAATT GCTGGCATCA ACAACAACAG CGCATGAGTA CAGCAGCACA 
ATGGCGACAG CGCGTGCTAG TGGTGCAACA ACACACACGG AAGGTGTTAT TAATACCCCA 
ACAACAACAA CAGTGAGTGA TAAAACATTG ACAACAACGA CAACGACAGC ACAACGCCAT 
TATCCCCTGC AGCAATTGCT GGGCAATAAA ACACTCTTGC CGCTGCACAA TCAACCGCCG 
TTGTTGCATT TCGATGGCAA CAGCAATCAC TTGGCACACA CACATCCACC CACAGCAACC 
ACGCTCCTCA CATCAACTTC CGCTGCCGCC GCTGCGCCAA CTTCAACAAC ACTTGGCGGC 
GACGCGACAA CTGCCGCGGC AGCGTTGACC ACCAAAAAGT CGATACGCAT CTCGACGCAG 
AAATTGTTGG CGACACCCTT CCATCAGCTC ACCTATGTGC GCAACAACGC CGGCGACATC 
GATATCGACA ATATGGACAA CATCAGCGTC TATCCGGATC TGCTGGAGGA GTCGGGCGAA 
TTGGCGGATC AACATTCGAC GGAGCGCGCC GAACCCGATG CGACACGCAA TAGCCGACTG 
ACAATCGTTA GCGGTTATCT GCCCACTTCG GCCACGGCGA CGCTACCCGA ATCGATACGT 
CTGGCTAAGA TTAAGATTAA CAAGGAGAGA AAGCGCATGA TGAGCATGAG AAAAAGACAC 
ACCAGGCGTT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105215648 ( XM_029042520.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028898353.1 Zeugodacus cucurbitae probable serine/threonine-protein kinase DDB_G0267686 (LOC105215648), mRNA.

Price & Availability↑

CloneID	OBa256865
Clone ID Related Accession (Same CDS sequence)	XM_029042520.1
Accession Version	XM_029042520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0267686
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863756.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC TGCGCGACAC CTGCTACTTC AACGAAACCA TCGTACACGG TTATTTCGTG 
TTTCGTTCCG TAGTCGATCT GCAGCGACGT GTCGGCTTCA CGCATCGCGG TAAGGCGGTC 
GGGCCGAAGA AGAATGTCAA CGATGCCTGC TATCTGTTCA CGAAGATACA GGCCGATCAG 
TTTTTCCATC AGCACACCGA TTATTTTGCA CCGAAATCGA AGAGCACCAA AATCATCAGC 
GGCATTGGTC ACTCACCGCC GCAAACAACA ACAACAGCCA CGCATACAAC ATCAGGCGCC 
TCAGCGCGAC TGGCGTCACA GCGCGCTGCA CTCTCGGCAG CTGCTACAAC GGCGACTGTC 
ACAGCAACAG CGGCGCAACG CAACAACAAC AGGCTATTGC GTAACAACAA CAACAAGAAT 
AAGCGAGTGA ATGGCATGCG CAGCGGCAAC AATAATAGCC GGAGCAGTAA CAATAACCAA 
AATGCAGAGT ACACTAATAA TGGCCATAAT AGCAATTTAA GCAGCAGCAG CAACAATAAT 
AATAACAAAA ACAACACCAA CAATCAGAGC GTTAATAATA ACCATAATAA CAACAGTAAT 
TTAGTTAGCA ATAGTCAGCA GCAGCAGCAG CAAGTAGATT TAAGTCAACA GAAGCAACAC 
AACAACAGCA ATGGTCGCAC GCGCGAACAT GTGCGTCAGC AACAACAACA TCAGCATCAA 
GTGCGGCATC ATCACAACGA TCCGAACTTG ATAGCACGTC GCCAACAACA CGAGCACAAA 
CAGAAGCGGC GACAGCATCA GCGCCAGCAG CAGCAGCGAC TGCGTAGCGG CAGAATAACT 
AGTCCAGCAA CAACAACAGT AGCGCTAAAT GACGCGCAAG AACGCGCCAC AACGACGAAA 
GCGCTTTACA GCACGGCGGC AACAGCTGCT GTGGCGCGCA GCAATCCGCA GCAGGAGAAA 
GGCACGCGTC ATGAACCGCA AGCGGCGACT GTTGCAAATG TGGCGGCTAA GGCGGCAACG 
GCAACTTCAA TAATTATGAA GTCAACAACA ACCATTGCCA ATAAGCGTAA GGGACGGCGA 
AGAAAGGGGC GAAAGCAACG AAAACAGCAG CAGCAACAAC AACCACAGCG CGCACAACAA 
CAAATGTCGC CGCTCGTCGC GCATGAACCC GCGACACCAA CGCAAACCAC TCGGCTGTGG 
AGCGATTCAC CAACGCCACC ACTTCCGTCG CTGCCAGCGC AGACGCAGGC CGATGTGCCG 
AGCAGCATAC CAACCGCAAC AACATACGGC GATTACAGCG GCGAATGGCA GTCCTCAGTG 
CCGACGTACG CCGACGCGTC GACCGTGGAC ACCGCGGCGT CTTCATCCTT AACACCAACA 
CTGTCTTCGG ACATACCTAC ACAAACATAC ACGGCCTCAA CAACCGATCT GATCGATGGT 
GCCTCGTCGC TCACGCCAAC AAGCGACTAC AACAACAACA CTGAATTCAC AAGCGAAACG 
CTGGCGTTTG CCACCGAAAT CGATGACAGT CGTATGACGC CGGAGTCTAC AATGTCGGCC 
GACGAACAAA TAAACAGTAT TACTTCCCAA CAACAACACT TTACAACACA AAATATGGCC 
ACGCCAGTGT CGACTGCGCA ATCCGTGGAG CCTACGTCAA CCACGTGGTC CGAGAGCAGT 
GAACACAGCT GGTCACCTAC CACTACAACC AATGAAACCG ATGATGGCGA GACACTGTTC 
GCCTCTCCGA CTGCAATGTT CGACGCGGAA AGCGTTGCAG CCACAGCAAT CTTCGCAGAG 
GACAACAATG CTGCAGAGGA AACGCAAACC GTTGCTGTGG ACACCGATTC ATTTGCAACA 
ATCAACACTA CCTCCAGTGA AATTGAAGCG GAAAGCAGCA GCAGCAATAG CAACACCAAC 
AACGACGGCT TCACTGAAGC GCTAGCGGAT GATGTTGCTG GCACGCAGAG CACAACAACA 
GCACACCACA GAGTCTTTAA TTTACCAGCT GAACGGGCGC ATATCGGTCA CATGTGGAAG 
CAGCAGTCGG GCATTGCGGC GAGCATAGCC TCGCTGTTGC ACAAACAACT GCTGACGACG 
AGCGCCAGCA GCGGCAACAA ATACGCTTGG CGGCCGCAGC GCGCTGAAGA ATACTCAGAT 
GAAATAATCG TGGCTACAGG ACGGCCAGTC GCGACTGAAA CGGAAACGAC AACCGATATT 
GCAGCGGGCG ATCGTGCAAC ATGGTGGCAA CACCATATGA ATCACAGCGG CACGAACTTT 
TGGGACAAAG GCATTGCGGA TGAGGTGCTA AGCAGCGGCG ACGATGCCGA TTTGGATTGG 
CTGAATCACC ACAACAAGCA CCGCAATGCA AGCAAGCGCC GGCATAACAA CCGCAAGGCC 
AACAACAGCC GAGATGTTGA AGCCGTGTCA CAGCAACCGT TGCAAGCGTT GTTGACGAGC 
GATTCGAGCC TTAGTTTTAG CAAAAAATAT TTAGCCGAAG GCAAATTACA ACGACAAAGT 
AGCAAAGCAG TAAGCGGCAA CAACAAATTG CTGGCATCAA CAACAACAGC GCATGAGTAC 
AGCAGCACAA TGGCGACAGC GCGTGCTAGT GGTGCAACAA CACACACGGA AGGTGTTATT 
AATACCCCAA CAACAACAAC AGTGAGTGAT AAAACATTGA CAACAACGAC AACGACAGCA 
CAACGCCATT ATCCCCTGCA GCAATTGCTG GGCAATAAAA CACTCTTGCC GCTGCACAAT 
CAACCGCCGT TGTTGCATTT CGATGGCAAC AGCAATCACT TGGCACACAC ACATCCACCC 
ACAGCAACCA CGCTCCTCAC ATCAACTTCC GCTGCCGCCG CTGCGCCAAC TTCAACAACA 
CTTGGCGGCG ACGCGACAAC TGCCGCGGCA GCGTTGACCA CCAAAAAGTC GATACGCATC 
TCGACGCAGA AATTGTTGGC GACACCCTTC CATCAGCTCA CCTATGTGCG CAACAACGCC 
GGCGACATCG ATATCGACAA TATGGACAAC ATCAGCGTCT ATCCGGATCT GCTGGAGGAG 
TCGGGCGAAT TGGCGGATCA ACATTCGACG GAGCGCGCCG AACCCGATGC GACACGCAAT 
AGCCGACTGA CAATCGTTAG CGGTTATCTG CCCACTTCGG CCACGGCGAC GCTACCCGAA 
TCGATACGTC TGGCTAAGAT TAAGATTAAC AAGGAGAGAA AGCGCATGAT GAGCATGAGA 
AAAAGACACA CCAGGCGTTA TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028898353.1
CDS	123..3389
Translation	MKELRDTCYFNETIVHGYFVFRSVVDLQRRVGFTHRGKAVGPKKNVNDACYLFTKIQADQFFHQHTDYFAPKSKSTKIISGIGHSPPQTTTTATHTTSGASARLASQRAALSAAATTATVTATAAQRNNNRLLRNNNNKNKRVNGMRSGNNNSRSSNNNQNAEYTNNGHNSNLSSSSNNNNNKNNTNNQSVNNNHNNNSNLVSNSQQQQQQVDLSQQKQHNNSNGRTREHVRQQQQHQHQVRHHHNDPNLIARRQQHEHKQKRRQHQRQQQQRLRSGRITSPATTTVALNDAQERATTTKALYSTAATAAVARSNPQQEKGTRHEPQAATVANVAAKAATATSIIMKSTTTIANKRKGRRRKGRKQRKQQQQQQPQRAQQQMSPLVAHEPATPTQTTRLWSDSPTPPLPSLPAQTQADVPSSIPTATTYGDYSGEWQSSVPTYADASTVDTAASSSLTPTLSSDIPTQTYTASTTDLIDGASSLTPTSDYNNNTEFTSETLAFATEIDDSRMTPESTMSADEQINSITSQQQHFTTQNMATPVSTAQSVEPTSTTWSESSEHSWSPTTTTNETDDGETLFASPTAMFDAESVAATAIFAEDNNAAEETQTVAVDTDSFATINTTSSEIEAESSSSNSNTNNDGFTEALADDVAGTQSTTTAHHRVFNLPAERAHIGHMWKQQSGIAASIASLLHKQLLTTSASSGNKYAWRPQRAEEYSDEIIVATGRPVATETETTTDIAAGDRATWWQHHMNHSGTNFWDKGIADEVLSSGDDADLDWLNHHNKHRNASKRRHNNRKANNSRDVEAVSQQPLQALLTSDSSLSFSKKYLAEGKLQRQSSKAVSGNNKLLASTTTAHEYSSTMATARASGATTHTEGVINTPTTTTVSDKTLTTTTTTAQRHYPLQQLLGNKTLLPLHNQPPLLHFDGNSNHLAHTHPPTATTLLTSTSAAAAAPTSTTLGGDATTAAAALTTKKSIRISTQKLLATPFHQLTYVRNNAGDIDIDNMDNISVYPDLLEESGELADQHSTERAEPDATRNSRLTIVSGYLPTSATATLPESIRLAKIKINKERKRMMSMRKRHTRRYA

Target ORF information:

RefSeq Version	XM_029042520.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae probable serine/threonine-protein kinase DDB_G0267686 (LOC105215648), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029042520.1

ORF Insert Sequence:

ATGAAAGAAC TGCGCGACAC CTGCTACTTC AACGAAACCA TCGTACACGG TTATTTCGTG 
TTTCGTTCCG TAGTCGATCT GCAGCGACGT GTCGGCTTCA CGCATCGCGG TAAGGCGGTC 
GGGCCGAAGA AGAATGTCAA CGATGCCTGC TATCTGTTCA CGAAGATACA GGCCGATCAG 
TTTTTCCATC AGCACACCGA TTATTTTGCA CCGAAATCGA AGAGCACCAA AATCATCAGC 
GGCATTGGTC ACTCACCGCC GCAAACAACA ACAACAGCCA CGCATACAAC ATCAGGCGCC 
TCAGCGCGAC TGGCGTCACA GCGCGCTGCA CTCTCGGCAG CTGCTACAAC GGCGACTGTC 
ACAGCAACAG CGGCGCAACG CAACAACAAC AGGCTATTGC GTAACAACAA CAACAAGAAT 
AAGCGAGTGA ATGGCATGCG CAGCGGCAAC AATAATAGCC GGAGCAGTAA CAATAACCAA 
AATGCAGAGT ACACTAATAA TGGCCATAAT AGCAATTTAA GCAGCAGCAG CAACAATAAT 
AATAACAAAA ACAACACCAA CAATCAGAGC GTTAATAATA ACCATAATAA CAACAGTAAT 
TTAGTTAGCA ATAGTCAGCA GCAGCAGCAG CAAGTAGATT TAAGTCAACA GAAGCAACAC 
AACAACAGCA ATGGTCGCAC GCGCGAACAT GTGCGTCAGC AACAACAACA TCAGCATCAA 
GTGCGGCATC ATCACAACGA TCCGAACTTG ATAGCACGTC GCCAACAACA CGAGCACAAA 
CAGAAGCGGC GACAGCATCA GCGCCAGCAG CAGCAGCGAC TGCGTAGCGG CAGAATAACT 
AGTCCAGCAA CAACAACAGT AGCGCTAAAT GACGCGCAAG AACGCGCCAC AACGACGAAA 
GCGCTTTACA GCACGGCGGC AACAGCTGCT GTGGCGCGCA GCAATCCGCA GCAGGAGAAA 
GGCACGCGTC ATGAACCGCA AGCGGCGACT GTTGCAAATG TGGCGGCTAA GGCGGCAACG 
GCAACTTCAA TAATTATGAA GTCAACAACA ACCATTGCCA ATAAGCGTAA GGGACGGCGA 
AGAAAGGGGC GAAAGCAACG AAAACAGCAG CAGCAACAAC AACCACAGCG CGCACAACAA 
CAAATGTCGC CGCTCGTCGC GCATGAACCC GCGACACCAA CGCAAACCAC TCGGCTGTGG 
AGCGATTCAC CAACGCCACC ACTTCCGTCG CTGCCAGCGC AGACGCAGGC CGATGTGCCG 
AGCAGCATAC CAACCGCAAC AACATACGGC GATTACAGCG GCGAATGGCA GTCCTCAGTG 
CCGACGTACG CCGACGCGTC GACCGTGGAC ACCGCGGCGT CTTCATCCTT AACACCAACA 
CTGTCTTCGG ACATACCTAC ACAAACATAC ACGGCCTCAA CAACCGATCT GATCGATGGT 
GCCTCGTCGC TCACGCCAAC AAGCGACTAC AACAACAACA CTGAATTCAC AAGCGAAACG 
CTGGCGTTTG CCACCGAAAT CGATGACAGT CGTATGACGC CGGAGTCTAC AATGTCGGCC 
GACGAACAAA TAAACAGTAT TACTTCCCAA CAACAACACT TTACAACACA AAATATGGCC 
ACGCCAGTGT CGACTGCGCA ATCCGTGGAG CCTACGTCAA CCACGTGGTC CGAGAGCAGT 
GAACACAGCT GGTCACCTAC CACTACAACC AATGAAACCG ATGATGGCGA GACACTGTTC 
GCCTCTCCGA CTGCAATGTT CGACGCGGAA AGCGTTGCAG CCACAGCAAT CTTCGCAGAG 
GACAACAATG CTGCAGAGGA AACGCAAACC GTTGCTGTGG ACACCGATTC ATTTGCAACA 
ATCAACACTA CCTCCAGTGA AATTGAAGCG GAAAGCAGCA GCAGCAATAG CAACACCAAC 
AACGACGGCT TCACTGAAGC GCTAGCGGAT GATGTTGCTG GCACGCAGAG CACAACAACA 
GCACACCACA GAGTCTTTAA TTTACCAGCT GAACGGGCGC ATATCGGTCA CATGTGGAAG 
CAGCAGTCGG GCATTGCGGC GAGCATAGCC TCGCTGTTGC ACAAACAACT GCTGACGACG 
AGCGCCAGCA GCGGCAACAA ATACGCTTGG CGGCCGCAGC GCGCTGAAGA ATACTCAGAT 
GAAATAATCG TGGCTACAGG ACGGCCAGTC GCGACTGAAA CGGAAACGAC AACCGATATT 
GCAGCGGGCG ATCGTGCAAC ATGGTGGCAA CACCATATGA ATCACAGCGG CACGAACTTT 
TGGGACAAAG GCATTGCGGA TGAGGTGCTA AGCAGCGGCG ACGATGCCGA TTTGGATTGG 
CTGAATCACC ACAACAAGCA CCGCAATGCA AGCAAGCGCC GGCATAACAA CCGCAAGGCC 
AACAACAGCC GAGATGTTGA AGCCGTGTCA CAGCAACCGT TGCAAGCGTT GTTGACGAGC 
GATTCGAGCC TTAGTTTTAG CAAAAAATAT TTAGCCGAAG GCAAATTACA ACGACAAAGT 
AGCAAAGCAG TAAGCGGCAA CAACAAATTG CTGGCATCAA CAACAACAGC GCATGAGTAC 
AGCAGCACAA TGGCGACAGC GCGTGCTAGT GGTGCAACAA CACACACGGA AGGTGTTATT 
AATACCCCAA CAACAACAAC AGTGAGTGAT AAAACATTGA CAACAACGAC AACGACAGCA 
CAACGCCATT ATCCCCTGCA GCAATTGCTG GGCAATAAAA CACTCTTGCC GCTGCACAAT 
CAACCGCCGT TGTTGCATTT CGATGGCAAC AGCAATCACT TGGCACACAC ACATCCACCC 
ACAGCAACCA CGCTCCTCAC ATCAACTTCC GCTGCCGCCG CTGCGCCAAC TTCAACAACA 
CTTGGCGGCG ACGCGACAAC TGCCGCGGCA GCGTTGACCA CCAAAAAGTC GATACGCATC 
TCGACGCAGA AATTGTTGGC GACACCCTTC CATCAGCTCA CCTATGTGCG CAACAACGCC 
GGCGACATCG ATATCGACAA TATGGACAAC ATCAGCGTCT ATCCGGATCT GCTGGAGGAG 
TCGGGCGAAT TGGCGGATCA ACATTCGACG GAGCGCGCCG AACCCGATGC GACACGCAAT 
AGCCGACTGA CAATCGTTAG CGGTTATCTG CCCACTTCGG CCACGGCGAC GCTACCCGAA 
TCGATACGTC TGGCTAAGAT TAAGATTAAC AAGGAGAGAA AGCGCATGAT GAGCATGAGA 
AAAAGACACA CCAGGCGTTA TGCGTAG