ube3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ube3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa41882 XM_018094690.1
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa00604 XM_012966190.3
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa00604 XM_031902395.1
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa00604 XM_031902387.1
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa00604 NM_001079407.1
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OXa00604 XM_012966190.2 Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa41882
    Clone ID Related Accession (Same CDS sequence) XM_018094690.1
    Accession Version XM_018094690.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGTCA CCTTTACAGA TACCCTAACC TGGAAAATAC TCAAGGGGAA AGGCGAAAGC 
    CTGAGACCAG CTATGAACCA CATTTGTGCA AATATTATGG GGTATCTTAA CCAGAAAGGA
    TTTTATTCTG TTCTACAGAT ACTGTTAACC AATGGCTTGG CCAGATCTCG CCCCTCCTTG
    TCCAAAGGCA CGCTTACAGC CCTGTTTTCA TTGGCGTTGC GTCCAGTAGT AGCAGCACAA
    TATTCTGACA ACCTTCTTCG ATCCTTCTTG ATCCACATAA TGTCTGTACC AGCTTTGGTT
    TCCCACTTGT CTACACTTAC TCCAGACAGG CTTTCCGTAA TAGAATCGAA TGGTCTCTTC
    CATAAATTCA TCTTGTTTCT GAGCCGGGAG GATCAGTGCA GTGACGTGTG TGAGTGCCTG
    GAGGGCAGCC ATACTCTTTG TTTGCTAGGT AACCTCATTC ACCTGGGCCA CCTGACAGAG
    AAGGTGTTGG AGGAGGAGAT GTTTCACTTT GTCAGTGTCC TTACCCAAAT GTTATCTTAT
    TGCCAGAAAT ATGTGTCTCA GAAGAAGTCC AATCTTACAC ACTGGCACCC TGTTCTGGGC
    TGGTTCTCCC AGACAGTGGA CTATGGTCTC AATGAGTCCA TGCCCTTACT CACCAAGCAA
    CTTCAACATC TGTGGGGTGT CCATATGATA CGTAATTTGT TTCGTGAGGT CCTAAGCAAG
    AAGTTGGCAG AAAACCAAGA CGTGTCTGTT CCTGTGTCAC AAAGCTCTTC TTCCTCCCCA
    CAGAATAATC TACCCATGAA GAATCTCTTT AAACGAGCGT TCCAGAAATC TGCTTCTGTT
    CGACACATCC TTAAACCTAT TGGGGGGAAA AAGGTAGATT CTCCAGAAGT GCAAAAAGTT
    TGCAACATCT GTGTTCTGTA CCAGATGGCA CTGACAACTC TGACACAGAT ACGGCTACAG
    ATCCTCACAG GGCTTACCTA TCTGGATGAC CTTTTGCCCA AGTTGTGGGC ATTCATATGT
    GAACTAGGAC CTCAGGGAGG TCTGAAACTC TTCTTGGAAT GTCTGAGTAG TGACACAGAG
    GAGTCCCGTA GACTTCTGGC TATGCTGGTT TTGTTTTGCG ACTGCTCTAG GCATTTAATT
    ACGATCTTGG ATGATATTGA GGTATATGAA GAACAGATAT CATTTAAACT AGAGGAGCTT
    GTGACTATTT CCTCCTTTCT GAACTCCTTT GTGTTCAAGA TGATCTGGGA TGGACTTCTT
    GAGAATGCCA AAGGAGAGAC TCTGGAGCTA TTCCACTCTG TGCATGGATG GCTCATGGTC
    CTGTATGAAA GAGACTGCCG TAGGCGTTTT GCCCCTGAGG ATCATTGGCT GCGCAAGGAT
    CTCAAACCAA CCCTGCTATT CCAAGAACTG GATAAAGAGA GAAAGAGAGC CCAGCTCTTA
    CTTCAGTATA TCCCTCATGT CATCCCACAC AAAAACAGGG TCCTCTTGTT CCGAAATATT
    GTCACCAAGG AAAAGGAGAA GTTGGGTTTA GTGGAAACTA ACACCGCATC CCCACATGTC
    ACCCACATTA CCATCCGAAG GTCACGTATG TTAGAGGATG GATACGACCA ACTAAGGCAG
    CTTTCCCAGA ATGCCATGAA AGGTGTTATA CGTGTAAAGT TTGTGAACGA TCTTGGTGTA
    GATGAAGCTG GTATAGACCA GGATGGTGTC TTTAAGGAGT TCTTAGAGGA AATTATCAAG
    AGAGTATTTG ACCCTGCACT GAATCTTTTT AAGACCACTA GTTCTGGAGA TAGGTTGTAC
    CCATCTCCTA CATCTTATAT ACATGAAAAC TACCTCCAGC TGTTTGAATT TGTAGGGAAG
    ATGCTGGGCA AAGCAGTGTA TGAGGGTATA GTGGTGGATG TCCCATTTGC ATCATTCTTT
    CTCAGCCAGC TTCTTGGGCA CCATCATAGC ATCTTCTACA GCTCTGTGGA TGAGCTGCCT
    TCCTTGGACT CTGAATTCTA CAAGAATCTC ACTTCCATCA AGCGCTACGA TGGGGATATT
    AGTGATTTGG GACTTACCCT TTCATACGAT GAGGATGTTA TGGGTCAGCT GGTCTGTCAT
    GAACTGGTCC CAGGTGGGAA GTCTATTCCA GTGACTAATG AAAATAAAAT CAGTTATATC
    CACCTCATGG CTCATTTCCG CATGCACACG CAGATAAAGA ACCAAACTGT TGCCTTCATT
    AGTGGATTCC GCTCCATCAT AAGGCCTGAA TGGATCCGCA TGTTCTCAGC ACCTGAACTG
    CAACGTCTCA TATCAGGGGA CAATGCAGAG ATTGACCTGG ATGATCTAAA GAAACACACA
    GTCTACTATG GAGGGTTTCA TGGAAGTCAT CGTGTCATTA TCTGGCTGTG GGATATTTTG
    GCCAATGATT TCTCTTCTGA AGAAAAAGCC ATGTTTCTTA AGTTTGTCAC CAGTTGCTCT
    CGGCCTCCTC TACTTGGATT TGCCTACCTC AAACCTCCCT TTTCCATTAG ATGTGTAGAG
    GTGTCAGATG ATCAGGATAC AGGCGACACC CTGGGCAGTG TCCTACGTGG GTTCTTCACC
    ATCCGAAAGA AAGAGCCTGG TGGACGCCTT CCCACTTCCT CCACCTGCTT CAATCTCCTG
    AAGCTTCCCA ACTACAGTAA AAAAGGAATT CTGAGGGAGA AACTACGCTA TGCTATCAGC
    ATGAACACTG GTTTTGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950179.1
    CDS129..2855
    Translation

    Target ORF information:

    RefSeq Version XM_018094690.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGTCA CCTTTACAGA TACCCTAACC TGGAAAATAC TCAAGGGGAA AGGCGAAAGC 
    CTGAGACCAG CTATGAACCA CATTTGTGCA AATATTATGG GGTATCTTAA CCAGAAAGGA
    TTTTATTCTG TTCTACAGAT ACTGTTAACC AATGGCTTGG CCAGATCTCG CCCCTCCTTG
    TCCAAAGGCA CGCTTACAGC CCTGTTTTCA TTGGCGTTGC GTCCAGTAGT AGCAGCACAA
    TATTCTGACA ACCTTCTTCG ATCCTTCTTG ATCCACATAA TGTCTGTACC AGCTTTGGTT
    TCCCACTTGT CTACACTTAC TCCAGACAGG CTTTCCGTAA TAGAATCGAA TGGTCTCTTC
    CATAAATTCA TCTTGTTTCT GAGCCGGGAG GATCAGTGCA GTGACGTGTG TGAGTGCCTG
    GAGGGCAGCC ATACTCTTTG TTTGCTAGGT AACCTCATTC ACCTGGGCCA CCTGACAGAG
    AAGGTGTTGG AGGAGGAGAT GTTTCACTTT GTCAGTGTCC TTACCCAAAT GTTATCTTAT
    TGCCAGAAAT ATGTGTCTCA GAAGAAGTCC AATCTTACAC ACTGGCACCC TGTTCTGGGC
    TGGTTCTCCC AGACAGTGGA CTATGGTCTC AATGAGTCCA TGCCCTTACT CACCAAGCAA
    CTTCAACATC TGTGGGGTGT CCATATGATA CGTAATTTGT TTCGTGAGGT CCTAAGCAAG
    AAGTTGGCAG AAAACCAAGA CGTGTCTGTT CCTGTGTCAC AAAGCTCTTC TTCCTCCCCA
    CAGAATAATC TACCCATGAA GAATCTCTTT AAACGAGCGT TCCAGAAATC TGCTTCTGTT
    CGACACATCC TTAAACCTAT TGGGGGGAAA AAGGTAGATT CTCCAGAAGT GCAAAAAGTT
    TGCAACATCT GTGTTCTGTA CCAGATGGCA CTGACAACTC TGACACAGAT ACGGCTACAG
    ATCCTCACAG GGCTTACCTA TCTGGATGAC CTTTTGCCCA AGTTGTGGGC ATTCATATGT
    GAACTAGGAC CTCAGGGAGG TCTGAAACTC TTCTTGGAAT GTCTGAGTAG TGACACAGAG
    GAGTCCCGTA GACTTCTGGC TATGCTGGTT TTGTTTTGCG ACTGCTCTAG GCATTTAATT
    ACGATCTTGG ATGATATTGA GGTATATGAA GAACAGATAT CATTTAAACT AGAGGAGCTT
    GTGACTATTT CCTCCTTTCT GAACTCCTTT GTGTTCAAGA TGATCTGGGA TGGACTTCTT
    GAGAATGCCA AAGGAGAGAC TCTGGAGCTA TTCCACTCTG TGCATGGATG GCTCATGGTC
    CTGTATGAAA GAGACTGCCG TAGGCGTTTT GCCCCTGAGG ATCATTGGCT GCGCAAGGAT
    CTCAAACCAA CCCTGCTATT CCAAGAACTG GATAAAGAGA GAAAGAGAGC CCAGCTCTTA
    CTTCAGTATA TCCCTCATGT CATCCCACAC AAAAACAGGG TCCTCTTGTT CCGAAATATT
    GTCACCAAGG AAAAGGAGAA GTTGGGTTTA GTGGAAACTA ACACCGCATC CCCACATGTC
    ACCCACATTA CCATCCGAAG GTCACGTATG TTAGAGGATG GATACGACCA ACTAAGGCAG
    CTTTCCCAGA ATGCCATGAA AGGTGTTATA CGTGTAAAGT TTGTGAACGA TCTTGGTGTA
    GATGAAGCTG GTATAGACCA GGATGGTGTC TTTAAGGAGT TCTTAGAGGA AATTATCAAG
    AGAGTATTTG ACCCTGCACT GAATCTTTTT AAGACCACTA GTTCTGGAGA TAGGTTGTAC
    CCATCTCCTA CATCTTATAT ACATGAAAAC TACCTCCAGC TGTTTGAATT TGTAGGGAAG
    ATGCTGGGCA AAGCAGTGTA TGAGGGTATA GTGGTGGATG TCCCATTTGC ATCATTCTTT
    CTCAGCCAGC TTCTTGGGCA CCATCATAGC ATCTTCTACA GCTCTGTGGA TGAGCTGCCT
    TCCTTGGACT CTGAATTCTA CAAGAATCTC ACTTCCATCA AGCGCTACGA TGGGGATATT
    AGTGATTTGG GACTTACCCT TTCATACGAT GAGGATGTTA TGGGTCAGCT GGTCTGTCAT
    GAACTGGTCC CAGGTGGGAA GTCTATTCCA GTGACTAATG AAAATAAAAT CAGTTATATC
    CACCTCATGG CTCATTTCCG CATGCACACG CAGATAAAGA ACCAAACTGT TGCCTTCATT
    AGTGGATTCC GCTCCATCAT AAGGCCTGAA TGGATCCGCA TGTTCTCAGC ACCTGAACTG
    CAACGTCTCA TATCAGGGGA CAATGCAGAG ATTGACCTGG ATGATCTAAA GAAACACACA
    GTCTACTATG GAGGGTTTCA TGGAAGTCAT CGTGTCATTA TCTGGCTGTG GGATATTTTG
    GCCAATGATT TCTCTTCTGA AGAAAAAGCC ATGTTTCTTA AGTTTGTCAC CAGTTGCTCT
    CGGCCTCCTC TACTTGGATT TGCCTACCTC AAACCTCCCT TTTCCATTAG ATGTGTAGAG
    GTGTCAGATG ATCAGGATAC AGGCGACACC CTGGGCAGTG TCCTACGTGG GTTCTTCACC
    ATCCGAAAGA AAGAGCCTGG TGGACGCCTT CCCACTTCCT CCACCTGCTT CAATCTCCTG
    AAGCTTCCCA ACTACAGTAA AAAAGGAATT CTGAGGGAGA AACTACGCTA TGCTATCAGC
    ATGAACACTG GTTTTGAGCT CTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00604
    Clone ID Related Accession (Same CDS sequence) XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
    Accession Version XM_012966190.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821644.1
    CDS330..3548
    Translation

    Target ORF information:

    RefSeq Version XM_012966190.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966190.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00604
    Clone ID Related Accession (Same CDS sequence) XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
    Accession Version XM_031902387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758247.1
    CDS476..3694
    Translation

    Target ORF information:

    RefSeq Version XM_031902387.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00604
    Clone ID Related Accession (Same CDS sequence) XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
    Accession Version XM_031902395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758255.1
    CDS253..3471
    Translation

    Target ORF information:

    RefSeq Version XM_031902395.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00604
    Clone ID Related Accession (Same CDS sequence) XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
    Accession Version NM_001079407.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124027.1. ##Evidence-Data-START## Transcript exon combination :: BC124027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072875.1
    CDS100..3318
    Translation

    Target ORF information:

    RefSeq Version NM_001079407.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079407.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00604
    Clone ID Related Accession (Same CDS sequence) XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
    Accession Version XM_012966190.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821644.1
    CDS330..3548
    Translation

    Target ORF information:

    RefSeq Version XM_012966190.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
    GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC
    AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT
    CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA
    CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC
    CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC
    GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC
    TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA
    GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG
    AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT
    AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT
    CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA
    GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA
    CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG
    AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG
    TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC
    CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA
    ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC
    CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA
    CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG
    GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT
    TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA
    TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA
    GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG
    ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG
    GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT
    AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT
    AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA
    CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG
    GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA
    TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG
    CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA
    GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG
    TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA
    TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC
    CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC
    GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG
    GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA
    GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA
    TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT
    GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG
    GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC
    GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG
    CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA
    ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT
    GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA
    GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA
    AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG
    TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC
    ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT
    AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT
    GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC
    TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC
    TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.