nsf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nsf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nsf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa23525 NM_001079320.1
Latest version!
Xenopus tropicalis N-ethylmaleimide sensitive factor (nsf), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa29626 XM_012952583.3
Latest version!
Xenopus tropicalis N-ethylmaleimide sensitive factor (nsf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
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OXa29626 XM_012952583.2 Xenopus tropicalis N-ethylmaleimide sensitive factor (nsf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa23525
    Clone ID Related Accession (Same CDS sequence) NM_001079320.1
    Accession Version NM_001079320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vesicle-fusing ATPase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121254.1. ##Evidence-Data-START## Transcript exon combination :: BC121254.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGTGC GGTCCATGCA AGCAGCGAGG TGCCCCACAG ATGAGCTTTC CATTACCAAC 
    TGTGCCGTGG TCAATGAGAA AGATTTCCAG TCTGGCCAGC ATGTGAATGT GAGGACTTCC
    CCAAACCATC GCTATATATT CACTGTCAAG ACCCATCACA CAATATTGCC AGGAAGCATT
    GCTTTCAGTC TCCCACAGAG GAAATGGGCT GGACTCTCCA TTGGACAAGA TGTGGAAGTC
    GGGGTATATA CTTTCGACAA GACCAAGCAG TGCATTGGCA CCATGACCAT AGAGATCGAC
    TTCCTGCAGA AGAAGAGCAT TGATTCCAAC CCTTATGACA CGGACAGAAT GGCAGCAGAA
    TTCATTCAGC AGTTCAACAA CCAGGCCTTC TCCGTTGGCC AGCAGCTGGT GTTTAGTTTC
    AATGACAAGC TTTTTGGGTT AGTGGTGAAA GACATAGAGG CCATGGATCC AAGCATTTTA
    AAGGGAGAAC AGTCAACAGG AAAGAGGCAG AAGATAGAAG TTGGACTGGT GGTTGGGAAC
    AGCCAGGTGG TGTTTGAGAA GGCTGACAGC TCTTCTCTGA ATCTCACAGG CAAATCAAAG
    ACAAGGCAGG ACCGTCAATC TATAATCAAC CCAGACTGGA ATTTTGAGAA GATGGGCATT
    GGAGGGCTGG ACAAGGAGTT CTCAGATATC TTCAGAAGAG CCTTTGCCTC CCGAGTCTTC
    CCACCAGAGA TTGTGGAGCA AATGGGCTGC AAGCATGTAA AAGGCATTCT TCTGTTCGGC
    CCACCTGGTT GTGGCAAGAC CCTGATGGCT CGACAGATCG GTAAAATGTT GAATGCCCGG
    GAACCCAAAG TGGTGAATGG CCCTGAAATT CTGAACAAGT ATGTGGGAGA GTCAGAGGCA
    AACATCCGCA AACTCTTTGC AGACGCAGAG GAGGAACAGA GAAGGCTGGG TGCCAATAGT
    GGGCTGCACA TAATCATCTT TGATGAAATA GATGCCATCT GCAAGCAAAG AGGTAGCATG
    GCTGGCAGCA CTGGGGTGCA TGATACTGTG GTCAACCAGC TGCTGTCCAA GATAGATGGT
    GTGGAGCAGC TCAACAACAT TCTAGTGATT GGTATGACTA ACAGGCCTGA TCTGATAGAT
    GAAGCTCTTC TGAGACCTGG AAGATTGGAA GTGAAGATGG AGATTGGGCT TCCTGACGAG
    AAGGGTCGCC TGCAGATATT GCACATCCAT ACAGCTCGGA TGAGGGAGCA CAGTCTTCTC
    TCATCAGATG TGGATGTTGG TGAACTGGCA CAAGAAACCA AAAATTTCTC TGGAGCAGAA
    CTGGAGGGGC TGGTGAGGGC AGCCCAGTCA ACAGCTATGA ATCGGCACAT CAAGGCAACC
    AACAAAGTGG AGGTTGATAT GGAAAAGGCA GAACGCCTGC AAGTGACAAA AGGCGATTTC
    TTCACTTCTT TAGGGAATGA CATAAAACCT GCTTTTGGAA CAAACCAAGA GGACTATGCC
    AGCTACATTA TGAATGGCAT TATTAAATGG GGCGACCCTG TTACTCGGGT TCTTGATGAT
    GGAGAGCTGT TAGTCCAGCA AACGAAAAAC AGTGATCGTA CTCCACTTGT TAGCGTTTTA
    CTTGAAGGAC CTCCACATAG TGGCAAAACA GCCCTGGCTG CCAAGATCTC AGAGGAATCT
    AACTTTCCTT TTATTAAGAT TTGCTCACCG GAGAAGATGA TTGGGTTCTC TGAGACAGCC
    AAGTGCCAGG CCATAAAGAA GATCTTTGAA GATGCGTACA AATCACAGCT GAGCTGTGTC
    GTGGTCGATG ACATTGAGAG GCTCCTAGAT TATGTCCCTA TTGGTCCTCG CTTCTCCAAC
    CTAGTGCTGC AGGCTCTACT TGTCCTTTTG AAGAAGGCCC CTCCTCAGGG TCGCAAGCTG
    CTAATTATTG GCACCACCAG CCGGAAGGAT GTGCTTCAGG AAATGGAAAT GCTTGATGCA
    TTTAGCACAA CCATTCGTGT CCCAAATATC GTCACAGGTG AACAACTCAT GGAAGCCTTA
    GAGCTCCTGG GAGGTTTCAA GGACAACGAA AGATCAGTAA TCGCTCAACA AGTTAAAGGC
    AAAAAAGTGT ATATTGGAAT CAAAAAACTG CTGATGCTTA TTGAGATGTC CATGCAGATG
    GATCCAGAAT ACCGGGTTAG CAAGTTCCTT GCTCTTCTCA AAGAAGAGGC AGCATGGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072788.1
    CDS17..2239
    Translation

    Target ORF information:

    RefSeq Version NM_001079320.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis N-ethylmaleimide sensitive factor (nsf), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGTGC GGTCCATGCA AGCAGCGAGG TGCCCCACAG ATGAGCTTTC CATTACCAAC 
    TGTGCCGTGG TCAATGAGAA AGATTTCCAG TCTGGCCAGC ATGTGAATGT GAGGACTTCC
    CCAAACCATC GCTATATATT CACTGTCAAG ACCCATCACA CAATATTGCC AGGAAGCATT
    GCTTTCAGTC TCCCACAGAG GAAATGGGCT GGACTCTCCA TTGGACAAGA TGTGGAAGTC
    GGGGTATATA CTTTCGACAA GACCAAGCAG TGCATTGGCA CCATGACCAT AGAGATCGAC
    TTCCTGCAGA AGAAGAGCAT TGATTCCAAC CCTTATGACA CGGACAGAAT GGCAGCAGAA
    TTCATTCAGC AGTTCAACAA CCAGGCCTTC TCCGTTGGCC AGCAGCTGGT GTTTAGTTTC
    AATGACAAGC TTTTTGGGTT AGTGGTGAAA GACATAGAGG CCATGGATCC AAGCATTTTA
    AAGGGAGAAC AGTCAACAGG AAAGAGGCAG AAGATAGAAG TTGGACTGGT GGTTGGGAAC
    AGCCAGGTGG TGTTTGAGAA GGCTGACAGC TCTTCTCTGA ATCTCACAGG CAAATCAAAG
    ACAAGGCAGG ACCGTCAATC TATAATCAAC CCAGACTGGA ATTTTGAGAA GATGGGCATT
    GGAGGGCTGG ACAAGGAGTT CTCAGATATC TTCAGAAGAG CCTTTGCCTC CCGAGTCTTC
    CCACCAGAGA TTGTGGAGCA AATGGGCTGC AAGCATGTAA AAGGCATTCT TCTGTTCGGC
    CCACCTGGTT GTGGCAAGAC CCTGATGGCT CGACAGATCG GTAAAATGTT GAATGCCCGG
    GAACCCAAAG TGGTGAATGG CCCTGAAATT CTGAACAAGT ATGTGGGAGA GTCAGAGGCA
    AACATCCGCA AACTCTTTGC AGACGCAGAG GAGGAACAGA GAAGGCTGGG TGCCAATAGT
    GGGCTGCACA TAATCATCTT TGATGAAATA GATGCCATCT GCAAGCAAAG AGGTAGCATG
    GCTGGCAGCA CTGGGGTGCA TGATACTGTG GTCAACCAGC TGCTGTCCAA GATAGATGGT
    GTGGAGCAGC TCAACAACAT TCTAGTGATT GGTATGACTA ACAGGCCTGA TCTGATAGAT
    GAAGCTCTTC TGAGACCTGG AAGATTGGAA GTGAAGATGG AGATTGGGCT TCCTGACGAG
    AAGGGTCGCC TGCAGATATT GCACATCCAT ACAGCTCGGA TGAGGGAGCA CAGTCTTCTC
    TCATCAGATG TGGATGTTGG TGAACTGGCA CAAGAAACCA AAAATTTCTC TGGAGCAGAA
    CTGGAGGGGC TGGTGAGGGC AGCCCAGTCA ACAGCTATGA ATCGGCACAT CAAGGCAACC
    AACAAAGTGG AGGTTGATAT GGAAAAGGCA GAACGCCTGC AAGTGACAAA AGGCGATTTC
    TTCACTTCTT TAGGGAATGA CATAAAACCT GCTTTTGGAA CAAACCAAGA GGACTATGCC
    AGCTACATTA TGAATGGCAT TATTAAATGG GGCGACCCTG TTACTCGGGT TCTTGATGAT
    GGAGAGCTGT TAGTCCAGCA AACGAAAAAC AGTGATCGTA CTCCACTTGT TAGCGTTTTA
    CTTGAAGGAC CTCCACATAG TGGCAAAACA GCCCTGGCTG CCAAGATCTC AGAGGAATCT
    AACTTTCCTT TTATTAAGAT TTGCTCACCG GAGAAGATGA TTGGGTTCTC TGAGACAGCC
    AAGTGCCAGG CCATAAAGAA GATCTTTGAA GATGCGTACA AATCACAGCT GAGCTGTGTC
    GTGGTCGATG ACATTGAGAG GCTCCTAGAT TATGTCCCTA TTGGTCCTCG CTTCTCCAAC
    CTAGTGCTGC AGGCTCTACT TGTCCTTTTG AAGAAGGCCC CTCCTCAGGG TCGCAAGCTG
    CTAATTATTG GCACCACCAG CCGGAAGGAT GTGCTTCAGG AAATGGAAAT GCTTGATGCA
    TTTAGCACAA CCATTCGTGT CCCAAATATC GTCACAGGTG AACAACTCAT GGAAGCCTTA
    GAGCTCCTGG GAGGTTTCAA GGACAACGAA AGATCAGTAA TCGCTCAACA AGTTAAAGGC
    AAAAAAGTGT ATATTGGAAT CAAAAAACTG CTGATGCTTA TTGAGATGTC CATGCAGATG
    GATCCAGAAT ACCGGGTTAG CAAGTTCCTT GCTCTTCTCA AAGAAGAGGC AGCATGGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29626
    Clone ID Related Accession (Same CDS sequence) XM_012952583.2 , XM_012952583.3
    Accession Version XM_012952583.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vesicle-fusing ATPase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGTGC GGTCCATGCA AGCAGCGAGG TGCCCCACAG ATGAGCTTTC CATTACCAAC 
    TGTGCCGTGG TCAATGAGAA AGATTTCCAG TCTGGCCAGC ATGTGAATGT GAGGACTTCC
    CCAAACCATC GCTATATATT CACTGTCAAG ACCCATCACA CAATATTGCC AGGAAGCATT
    GCTTTCAGTC TCCCACAGAG GAAATGGGCT GGACTCTCCA TTGGACAAGA TGTGGAAGTC
    GGGGTATATA CTTTCGACAA GACCAAGCAG TGCATTGGCA CCATGACCAT AGAGATCGAC
    TTCCTGCAGA AGAAGAGCAT TGATTCCAAC CCTTATGACA CGGACAGAAT GGCAGCAGAA
    TTCATTCAGC AGTTCAACAA CCAGGCCTTC TCCGTTGGCC AGCAGCTGGT GTTTAGTTTC
    AATGACAAGC TTTTTGGGTT AGTGGTGAAA GACATAGAGG CCATGGATCC AAGCATTTTA
    AAGGGAGAAC AGTCAACAGG AAAGAGGCAG AAGATAGAAG TTGGACTGGT GGTTGGGAAC
    AGCCAGGTGG TGTTTGAGAA GGCTGACAGC TCTTCTCTGA ATCTCACAGG CAAATCAAAG
    ACAAGGCAGG ACCGTCAATC TATAATCAAC CCAGACTGGA ATTTTGAGAA GATGGGCATT
    GGAGGGCTGG ACAAGGAGTT CTCAGATATC TTCAGAAGAG CCTTTGCCTC CCGAGTCTTC
    CCACCAGAGA TTGTGGAGCA AATGGGCTGC AAGCATGTAA AAGGCATTCT TCTGTTTGGC
    CCACCTGGTT GTGGCAAGAC CCTGATGGCT CGACAGATCG GTAAAATGTT GAATGCCCGG
    GAACCCAAAG TGGTGAATGG CCCTGAAATT CTGAACAAGT ATGTGGGAGA GTCAGAGGCA
    AACATCCGCA AACTCTTTGC AGACGCAGAG GAGGAACAGA GAAGGCTGGG TGCCAATAGT
    GGGCTGCACA TAATCATCTT TGATGAAATA GATGCCATCT GCAAGCAAAG AGGTAGCATG
    GCTGGCAGCA CTGGGGTGCA TGATACTGTG GTCAACCAGC TGCTGTCCAA GATAGATGGT
    GTGGAGCAGC TCAACAACAT TCTAGTGATT GGTATGACTA ACAGGCCTGA TCTGATAGAT
    GAAGCTCTTC TGAGACCTGG AAGATTGGAA GTGAAGATGG AGATTGGGCT TCCTGACGAG
    AAGGGTCGCC TGCAGATATT GCACATCCAT ACAGCTCGGA TGAGGGAGCA CAGTCTTCTC
    TCATCAGATG TGGATGTTGG TGAACTGGCA CAAGAAACCA AAAATTTCTC TGGAGCAGAA
    CTGGAGGGGC TGGTGAGGGC AGCCCAGTCA ACAGCTATGA ATCGGCACAT CAAGGCAACC
    AACAAAGTGG AGGTTGATAT GGAAAAGGCA GAACGCCTGC AAGTGACAAA AGGCGATTTC
    TTCACTTCTT TAGGGAATGA CATAAAACCT GCTTTTGGAA CAAACCAAGA GGACTATGCC
    AGCTACATTA TGAATGGCAT TATTAAATGG GGCGACCCTG TTACTCGGGT TCTTGATGAT
    GGAGAGCTGT TAGTCCAGCA AACGAAAAAC AGTGATCGTA CTCCACTTGT TAGCGTTTTA
    CTTGAAGGAC CTCCACATAG TGGCAAAACA GCCCTGGCTG CCAAGATCTC AGAGGAATCT
    AACTTTCCTT TTATTAAGAT TTGCTCACCG GAGAAGATGA TTGGGTTCTC TGAGACAGCC
    AAGTGCCAGG CCATAAAGAA GATCTTTGAA GATGCGTACA AATCACAGCT GAGCTGTGTC
    GTGGTCGATG ACATTGAGAG GCTCCTAGAT TATGTCCCTA TTGGTCCTCG CTTCTCCAAC
    CTAGTGCTGC AGGCTCTACT TGTCCTTTTG AAGAAGGCCC CTCCTCAGGG TCGCAAGCTG
    CTAATTATTG GCACCACCAG CCGGAAGGAT GTGCTTCAGG AAATGGAAAT GCTTGATGCA
    TTTAGCACAA CCATTCGTGT CCCAAATATC GTCACAGGTG AACAACTCAT GGAAGCCTTA
    GAGCTCCTGG GAGGTTTCAA GGACAACGAA AGATCAGTAA TCGCTCAACA AGTTAAAGGC
    AAAAAAGTGT ATATTGGAAT CAAAAAACTG CTGATGCTTA TTGAGATGTC CATGCAGATG
    GATCCAGAAT ACCGGGTTAG CAAGTTCCTT GCTCTTCTCA AAGAAGAGGC AGCAGAGCCC
    GTCCATCGTG GCTATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808037.1
    CDS101..2341
    Translation

    Target ORF information:

    RefSeq Version XM_012952583.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis N-ethylmaleimide sensitive factor (nsf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952583.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGTGC GGTCCATGCA AGCAGCGAGG TGCCCCACAG ATGAGCTTTC CATTACCAAC 
    TGTGCCGTGG TCAATGAGAA AGATTTCCAG TCTGGCCAGC ATGTGAATGT GAGGACTTCC
    CCAAACCATC GCTATATATT CACTGTCAAG ACCCATCACA CAATATTGCC AGGAAGCATT
    GCTTTCAGTC TCCCACAGAG GAAATGGGCT GGACTCTCCA TTGGACAAGA TGTGGAAGTC
    GGGGTATATA CTTTCGACAA GACCAAGCAG TGCATTGGCA CCATGACCAT AGAGATCGAC
    TTCCTGCAGA AGAAGAGCAT TGATTCCAAC CCTTATGACA CGGACAGAAT GGCAGCAGAA
    TTCATTCAGC AGTTCAACAA CCAGGCCTTC TCCGTTGGCC AGCAGCTGGT GTTTAGTTTC
    AATGACAAGC TTTTTGGGTT AGTGGTGAAA GACATAGAGG CCATGGATCC AAGCATTTTA
    AAGGGAGAAC AGTCAACAGG AAAGAGGCAG AAGATAGAAG TTGGACTGGT GGTTGGGAAC
    AGCCAGGTGG TGTTTGAGAA GGCTGACAGC TCTTCTCTGA ATCTCACAGG CAAATCAAAG
    ACAAGGCAGG ACCGTCAATC TATAATCAAC CCAGACTGGA ATTTTGAGAA GATGGGCATT
    GGAGGGCTGG ACAAGGAGTT CTCAGATATC TTCAGAAGAG CCTTTGCCTC CCGAGTCTTC
    CCACCAGAGA TTGTGGAGCA AATGGGCTGC AAGCATGTAA AAGGCATTCT TCTGTTTGGC
    CCACCTGGTT GTGGCAAGAC CCTGATGGCT CGACAGATCG GTAAAATGTT GAATGCCCGG
    GAACCCAAAG TGGTGAATGG CCCTGAAATT CTGAACAAGT ATGTGGGAGA GTCAGAGGCA
    AACATCCGCA AACTCTTTGC AGACGCAGAG GAGGAACAGA GAAGGCTGGG TGCCAATAGT
    GGGCTGCACA TAATCATCTT TGATGAAATA GATGCCATCT GCAAGCAAAG AGGTAGCATG
    GCTGGCAGCA CTGGGGTGCA TGATACTGTG GTCAACCAGC TGCTGTCCAA GATAGATGGT
    GTGGAGCAGC TCAACAACAT TCTAGTGATT GGTATGACTA ACAGGCCTGA TCTGATAGAT
    GAAGCTCTTC TGAGACCTGG AAGATTGGAA GTGAAGATGG AGATTGGGCT TCCTGACGAG
    AAGGGTCGCC TGCAGATATT GCACATCCAT ACAGCTCGGA TGAGGGAGCA CAGTCTTCTC
    TCATCAGATG TGGATGTTGG TGAACTGGCA CAAGAAACCA AAAATTTCTC TGGAGCAGAA
    CTGGAGGGGC TGGTGAGGGC AGCCCAGTCA ACAGCTATGA ATCGGCACAT CAAGGCAACC
    AACAAAGTGG AGGTTGATAT GGAAAAGGCA GAACGCCTGC AAGTGACAAA AGGCGATTTC
    TTCACTTCTT TAGGGAATGA CATAAAACCT GCTTTTGGAA CAAACCAAGA GGACTATGCC
    AGCTACATTA TGAATGGCAT TATTAAATGG GGCGACCCTG TTACTCGGGT TCTTGATGAT
    GGAGAGCTGT TAGTCCAGCA AACGAAAAAC AGTGATCGTA CTCCACTTGT TAGCGTTTTA
    CTTGAAGGAC CTCCACATAG TGGCAAAACA GCCCTGGCTG CCAAGATCTC AGAGGAATCT
    AACTTTCCTT TTATTAAGAT TTGCTCACCG GAGAAGATGA TTGGGTTCTC TGAGACAGCC
    AAGTGCCAGG CCATAAAGAA GATCTTTGAA GATGCGTACA AATCACAGCT GAGCTGTGTC
    GTGGTCGATG ACATTGAGAG GCTCCTAGAT TATGTCCCTA TTGGTCCTCG CTTCTCCAAC
    CTAGTGCTGC AGGCTCTACT TGTCCTTTTG AAGAAGGCCC CTCCTCAGGG TCGCAAGCTG
    CTAATTATTG GCACCACCAG CCGGAAGGAT GTGCTTCAGG AAATGGAAAT GCTTGATGCA
    TTTAGCACAA CCATTCGTGT CCCAAATATC GTCACAGGTG AACAACTCAT GGAAGCCTTA
    GAGCTCCTGG GAGGTTTCAA GGACAACGAA AGATCAGTAA TCGCTCAACA AGTTAAAGGC
    AAAAAAGTGT ATATTGGAAT CAAAAAACTG CTGATGCTTA TTGAGATGTC CATGCAGATG
    GATCCAGAAT ACCGGGTTAG CAAGTTCCTT GCTCTTCTCA AAGAAGAGGC AGCAGAGCCC
    GTCCATCGTG GCTATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29626
    Clone ID Related Accession (Same CDS sequence) XM_012952583.2 , XM_012952583.3
    Accession Version XM_012952583.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vesicle-fusing ATPase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGTGC GGTCCATGCA AGCAGCGAGG TGCCCCACAG ATGAGCTTTC CATTACCAAC 
    TGTGCCGTGG TCAATGAGAA AGATTTCCAG TCTGGCCAGC ATGTGAATGT GAGGACTTCC
    CCAAACCATC GCTATATATT CACTGTCAAG ACCCATCACA CAATATTGCC AGGAAGCATT
    GCTTTCAGTC TCCCACAGAG GAAATGGGCT GGACTCTCCA TTGGACAAGA TGTGGAAGTC
    GGGGTATATA CTTTCGACAA GACCAAGCAG TGCATTGGCA CCATGACCAT AGAGATCGAC
    TTCCTGCAGA AGAAGAGCAT TGATTCCAAC CCTTATGACA CGGACAGAAT GGCAGCAGAA
    TTCATTCAGC AGTTCAACAA CCAGGCCTTC TCCGTTGGCC AGCAGCTGGT GTTTAGTTTC
    AATGACAAGC TTTTTGGGTT AGTGGTGAAA GACATAGAGG CCATGGATCC AAGCATTTTA
    AAGGGAGAAC AGTCAACAGG AAAGAGGCAG AAGATAGAAG TTGGACTGGT GGTTGGGAAC
    AGCCAGGTGG TGTTTGAGAA GGCTGACAGC TCTTCTCTGA ATCTCACAGG CAAATCAAAG
    ACAAGGCAGG ACCGTCAATC TATAATCAAC CCAGACTGGA ATTTTGAGAA GATGGGCATT
    GGAGGGCTGG ACAAGGAGTT CTCAGATATC TTCAGAAGAG CCTTTGCCTC CCGAGTCTTC
    CCACCAGAGA TTGTGGAGCA AATGGGCTGC AAGCATGTAA AAGGCATTCT TCTGTTTGGC
    CCACCTGGTT GTGGCAAGAC CCTGATGGCT CGACAGATCG GTAAAATGTT GAATGCCCGG
    GAACCCAAAG TGGTGAATGG CCCTGAAATT CTGAACAAGT ATGTGGGAGA GTCAGAGGCA
    AACATCCGCA AACTCTTTGC AGACGCAGAG GAGGAACAGA GAAGGCTGGG TGCCAATAGT
    GGGCTGCACA TAATCATCTT TGATGAAATA GATGCCATCT GCAAGCAAAG AGGTAGCATG
    GCTGGCAGCA CTGGGGTGCA TGATACTGTG GTCAACCAGC TGCTGTCCAA GATAGATGGT
    GTGGAGCAGC TCAACAACAT TCTAGTGATT GGTATGACTA ACAGGCCTGA TCTGATAGAT
    GAAGCTCTTC TGAGACCTGG AAGATTGGAA GTGAAGATGG AGATTGGGCT TCCTGACGAG
    AAGGGTCGCC TGCAGATATT GCACATCCAT ACAGCTCGGA TGAGGGAGCA CAGTCTTCTC
    TCATCAGATG TGGATGTTGG TGAACTGGCA CAAGAAACCA AAAATTTCTC TGGAGCAGAA
    CTGGAGGGGC TGGTGAGGGC AGCCCAGTCA ACAGCTATGA ATCGGCACAT CAAGGCAACC
    AACAAAGTGG AGGTTGATAT GGAAAAGGCA GAACGCCTGC AAGTGACAAA AGGCGATTTC
    TTCACTTCTT TAGGGAATGA CATAAAACCT GCTTTTGGAA CAAACCAAGA GGACTATGCC
    AGCTACATTA TGAATGGCAT TATTAAATGG GGCGACCCTG TTACTCGGGT TCTTGATGAT
    GGAGAGCTGT TAGTCCAGCA AACGAAAAAC AGTGATCGTA CTCCACTTGT TAGCGTTTTA
    CTTGAAGGAC CTCCACATAG TGGCAAAACA GCCCTGGCTG CCAAGATCTC AGAGGAATCT
    AACTTTCCTT TTATTAAGAT TTGCTCACCG GAGAAGATGA TTGGGTTCTC TGAGACAGCC
    AAGTGCCAGG CCATAAAGAA GATCTTTGAA GATGCGTACA AATCACAGCT GAGCTGTGTC
    GTGGTCGATG ACATTGAGAG GCTCCTAGAT TATGTCCCTA TTGGTCCTCG CTTCTCCAAC
    CTAGTGCTGC AGGCTCTACT TGTCCTTTTG AAGAAGGCCC CTCCTCAGGG TCGCAAGCTG
    CTAATTATTG GCACCACCAG CCGGAAGGAT GTGCTTCAGG AAATGGAAAT GCTTGATGCA
    TTTAGCACAA CCATTCGTGT CCCAAATATC GTCACAGGTG AACAACTCAT GGAAGCCTTA
    GAGCTCCTGG GAGGTTTCAA GGACAACGAA AGATCAGTAA TCGCTCAACA AGTTAAAGGC
    AAAAAAGTGT ATATTGGAAT CAAAAAACTG CTGATGCTTA TTGAGATGTC CATGCAGATG
    GATCCAGAAT ACCGGGTTAG CAAGTTCCTT GCTCTTCTCA AAGAAGAGGC AGCAGAGCCC
    GTCCATCGTG GCTATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808037.1
    CDS97..2337
    Translation

    Target ORF information:

    RefSeq Version XM_012952583.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis N-ethylmaleimide sensitive factor (nsf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952583.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGTGC GGTCCATGCA AGCAGCGAGG TGCCCCACAG ATGAGCTTTC CATTACCAAC 
    TGTGCCGTGG TCAATGAGAA AGATTTCCAG TCTGGCCAGC ATGTGAATGT GAGGACTTCC
    CCAAACCATC GCTATATATT CACTGTCAAG ACCCATCACA CAATATTGCC AGGAAGCATT
    GCTTTCAGTC TCCCACAGAG GAAATGGGCT GGACTCTCCA TTGGACAAGA TGTGGAAGTC
    GGGGTATATA CTTTCGACAA GACCAAGCAG TGCATTGGCA CCATGACCAT AGAGATCGAC
    TTCCTGCAGA AGAAGAGCAT TGATTCCAAC CCTTATGACA CGGACAGAAT GGCAGCAGAA
    TTCATTCAGC AGTTCAACAA CCAGGCCTTC TCCGTTGGCC AGCAGCTGGT GTTTAGTTTC
    AATGACAAGC TTTTTGGGTT AGTGGTGAAA GACATAGAGG CCATGGATCC AAGCATTTTA
    AAGGGAGAAC AGTCAACAGG AAAGAGGCAG AAGATAGAAG TTGGACTGGT GGTTGGGAAC
    AGCCAGGTGG TGTTTGAGAA GGCTGACAGC TCTTCTCTGA ATCTCACAGG CAAATCAAAG
    ACAAGGCAGG ACCGTCAATC TATAATCAAC CCAGACTGGA ATTTTGAGAA GATGGGCATT
    GGAGGGCTGG ACAAGGAGTT CTCAGATATC TTCAGAAGAG CCTTTGCCTC CCGAGTCTTC
    CCACCAGAGA TTGTGGAGCA AATGGGCTGC AAGCATGTAA AAGGCATTCT TCTGTTTGGC
    CCACCTGGTT GTGGCAAGAC CCTGATGGCT CGACAGATCG GTAAAATGTT GAATGCCCGG
    GAACCCAAAG TGGTGAATGG CCCTGAAATT CTGAACAAGT ATGTGGGAGA GTCAGAGGCA
    AACATCCGCA AACTCTTTGC AGACGCAGAG GAGGAACAGA GAAGGCTGGG TGCCAATAGT
    GGGCTGCACA TAATCATCTT TGATGAAATA GATGCCATCT GCAAGCAAAG AGGTAGCATG
    GCTGGCAGCA CTGGGGTGCA TGATACTGTG GTCAACCAGC TGCTGTCCAA GATAGATGGT
    GTGGAGCAGC TCAACAACAT TCTAGTGATT GGTATGACTA ACAGGCCTGA TCTGATAGAT
    GAAGCTCTTC TGAGACCTGG AAGATTGGAA GTGAAGATGG AGATTGGGCT TCCTGACGAG
    AAGGGTCGCC TGCAGATATT GCACATCCAT ACAGCTCGGA TGAGGGAGCA CAGTCTTCTC
    TCATCAGATG TGGATGTTGG TGAACTGGCA CAAGAAACCA AAAATTTCTC TGGAGCAGAA
    CTGGAGGGGC TGGTGAGGGC AGCCCAGTCA ACAGCTATGA ATCGGCACAT CAAGGCAACC
    AACAAAGTGG AGGTTGATAT GGAAAAGGCA GAACGCCTGC AAGTGACAAA AGGCGATTTC
    TTCACTTCTT TAGGGAATGA CATAAAACCT GCTTTTGGAA CAAACCAAGA GGACTATGCC
    AGCTACATTA TGAATGGCAT TATTAAATGG GGCGACCCTG TTACTCGGGT TCTTGATGAT
    GGAGAGCTGT TAGTCCAGCA AACGAAAAAC AGTGATCGTA CTCCACTTGT TAGCGTTTTA
    CTTGAAGGAC CTCCACATAG TGGCAAAACA GCCCTGGCTG CCAAGATCTC AGAGGAATCT
    AACTTTCCTT TTATTAAGAT TTGCTCACCG GAGAAGATGA TTGGGTTCTC TGAGACAGCC
    AAGTGCCAGG CCATAAAGAA GATCTTTGAA GATGCGTACA AATCACAGCT GAGCTGTGTC
    GTGGTCGATG ACATTGAGAG GCTCCTAGAT TATGTCCCTA TTGGTCCTCG CTTCTCCAAC
    CTAGTGCTGC AGGCTCTACT TGTCCTTTTG AAGAAGGCCC CTCCTCAGGG TCGCAAGCTG
    CTAATTATTG GCACCACCAG CCGGAAGGAT GTGCTTCAGG AAATGGAAAT GCTTGATGCA
    TTTAGCACAA CCATTCGTGT CCCAAATATC GTCACAGGTG AACAACTCAT GGAAGCCTTA
    GAGCTCCTGG GAGGTTTCAA GGACAACGAA AGATCAGTAA TCGCTCAACA AGTTAAAGGC
    AAAAAAGTGT ATATTGGAAT CAAAAAACTG CTGATGCTTA TTGAGATGTC CATGCAGATG
    GATCCAGAAT ACCGGGTTAG CAAGTTCCTT GCTCTTCTCA AAGAAGAGGC AGCAGAGCCC
    GTCCATCGTG GCTATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.