madd cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following madd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the madd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa56194 XM_012961370.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56194 XM_012961368.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56195 XM_012961371.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56196 XM_012961372.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56197 XM_031900232.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56198 XM_012961373.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56199 XM_012961375.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56200 XM_031900233.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56201 XM_012961376.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56202 XM_031900234.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56203 XM_012961377.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56204 XM_031900235.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56205 XM_012961378.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56206 XM_031900236.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56207 XM_031900237.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56208 XM_031900238.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56209 XM_031900239.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56210 XM_012961380.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56211 XM_012961381.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56212 XM_031900240.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56213 XM_031900241.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56214 XM_012961383.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56215 XM_031900242.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56216 XM_012961384.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56217 XM_012961386.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56218 XM_012961387.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56219 XM_012961388.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56220 XM_031900243.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56221 XM_012961390.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56222 XM_012961391.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56223 XM_031900244.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56224 XM_012961393.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56225 XM_031900245.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56226 XM_031900246.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56227 XM_012961394.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56228 XM_031900247.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X36, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56229 XM_031900248.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X37, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56230 XM_031900249.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56231 XM_031900250.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X39, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56232 XM_012961397.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56233 XM_031900251.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X41, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56234 XM_031900252.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56235 XM_031900253.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X43, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56236 XM_012961398.3
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X44, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56237 XM_031900254.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X45, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56238 XM_031900255.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X46, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56239 XM_031900256.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X47, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56240 XM_031900257.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X48, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa56241 XM_018093105.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X49, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa22139 NM_001079249.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29527 XM_012961370.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29527 XM_012961368.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29528 XM_012961371.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29529 XM_012961372.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29530 XM_012961373.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29531 XM_012961375.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29532 XM_012961376.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29533 XM_012961377.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29534 XM_012961378.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29535 XM_012961379.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29536 XM_012961380.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29537 XM_012961381.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29538 XM_012961382.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29539 XM_012961383.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29540 XM_012961384.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29541 XM_012961386.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29542 XM_012961387.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29543 XM_012961388.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29544 XM_012961389.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29545 XM_012961390.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29546 XM_012961391.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29547 XM_012961392.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29548 XM_012961393.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29549 XM_012961394.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29550 XM_012961395.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29551 XM_012961397.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29552 XM_012961398.2
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29553 XM_018093105.1
Latest version!
Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa56194
    Clone ID Related Accession (Same CDS sequence) XM_012961368.3 , XM_012961370.3
    Accession Version XM_012961368.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961368.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816822.1
    CDS343..5331
    Translation

    Target ORF information:

    RefSeq Version XM_012961368.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961368.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56194
    Clone ID Related Accession (Same CDS sequence) XM_012961368.3 , XM_012961370.3
    Accession Version XM_012961370.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961370.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816824.1
    CDS570..5558
    Translation

    Target ORF information:

    RefSeq Version XM_012961370.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961370.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56195
    Clone ID Related Accession (Same CDS sequence) XM_012961371.3
    Accession Version XM_012961371.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961371.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816825.1
    CDS343..5328
    Translation

    Target ORF information:

    RefSeq Version XM_012961371.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961371.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56196
    Clone ID Related Accession (Same CDS sequence) XM_012961372.3
    Accession Version XM_012961372.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961372.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816826.1
    CDS343..5322
    Translation

    Target ORF information:

    RefSeq Version XM_012961372.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961372.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56197
    Clone ID Related Accession (Same CDS sequence) XM_031900232.1
    Accession Version XM_031900232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGTTTTCAT ACAGCTGAAT CATATTAAAA AGTGTAACAC TGTACGGGGC
    GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAATACAAG
    ACACCCATGG CCCATGAGAT CTGCTACTCC GTCCTGTGTC TTTTCTCCTA CGTGGCCGCC
    ATCAGAGGAA AGGAGGCAGA GCTGAAGAGT AGACCCCCTC GGCCTGTGTC AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756092.1
    CDS343..5319
    Translation

    Target ORF information:

    RefSeq Version XM_031900232.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGTTTTCAT ACAGCTGAAT CATATTAAAA AGTGTAACAC TGTACGGGGC
    GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAATACAAG
    ACACCCATGG CCCATGAGAT CTGCTACTCC GTCCTGTGTC TTTTCTCCTA CGTGGCCGCC
    ATCAGAGGAA AGGAGGCAGA GCTGAAGAGT AGACCCCCTC GGCCTGTGTC AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56198
    Clone ID Related Accession (Same CDS sequence) XM_012961373.3
    Accession Version XM_012961373.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961373.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816827.1
    CDS343..5277
    Translation

    Target ORF information:

    RefSeq Version XM_012961373.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961373.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56199
    Clone ID Related Accession (Same CDS sequence) XM_012961375.3
    Accession Version XM_012961375.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961375.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816829.1
    CDS343..5274
    Translation

    Target ORF information:

    RefSeq Version XM_012961375.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961375.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56200
    Clone ID Related Accession (Same CDS sequence) XM_031900233.1
    Accession Version XM_031900233.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756093.1
    CDS343..5274
    Translation

    Target ORF information:

    RefSeq Version XM_031900233.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900233.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56201
    Clone ID Related Accession (Same CDS sequence) XM_012961376.3
    Accession Version XM_012961376.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961376.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816830.1
    CDS343..5271
    Translation

    Target ORF information:

    RefSeq Version XM_012961376.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961376.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56202
    Clone ID Related Accession (Same CDS sequence) XM_031900234.1
    Accession Version XM_031900234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756094.1
    CDS343..5271
    Translation

    Target ORF information:

    RefSeq Version XM_031900234.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56203
    Clone ID Related Accession (Same CDS sequence) XM_012961377.3
    Accession Version XM_012961377.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816831.1
    CDS343..5268
    Translation

    Target ORF information:

    RefSeq Version XM_012961377.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961377.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56204
    Clone ID Related Accession (Same CDS sequence) XM_031900235.1
    Accession Version XM_031900235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756095.1
    CDS343..5268
    Translation

    Target ORF information:

    RefSeq Version XM_031900235.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56205
    Clone ID Related Accession (Same CDS sequence) XM_012961378.3
    Accession Version XM_012961378.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816832.1
    CDS343..5265
    Translation

    Target ORF information:

    RefSeq Version XM_012961378.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961378.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56206
    Clone ID Related Accession (Same CDS sequence) XM_031900236.1
    Accession Version XM_031900236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756096.1
    CDS343..5265
    Translation

    Target ORF information:

    RefSeq Version XM_031900236.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56207
    Clone ID Related Accession (Same CDS sequence) XM_031900237.1
    Accession Version XM_031900237.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756097.1
    CDS343..5262
    Translation

    Target ORF information:

    RefSeq Version XM_031900237.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56208
    Clone ID Related Accession (Same CDS sequence) XM_031900238.1
    Accession Version XM_031900238.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGTTTTCAT ACAGCTGAAT CATATTAAAA AGTGTAACAC TGTACGGGGC
    GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAATACAAG
    ACACCCATGG CCCATGAGAT CTGCTACTCC GTCCTGTGTC TTTTCTCCTA CGTGGCCGCC
    ATCAGAGGAA AGGAGGCAGA GCTGAAGAGT AGACCCCCTC GGCCTGTGTC AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756098.1
    CDS343..5259
    Translation

    Target ORF information:

    RefSeq Version XM_031900238.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGTTTTCAT ACAGCTGAAT CATATTAAAA AGTGTAACAC TGTACGGGGC
    GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAATACAAG
    ACACCCATGG CCCATGAGAT CTGCTACTCC GTCCTGTGTC TTTTCTCCTA CGTGGCCGCC
    ATCAGAGGAA AGGAGGCAGA GCTGAAGAGT AGACCCCCTC GGCCTGTGTC AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56209
    Clone ID Related Accession (Same CDS sequence) XM_031900239.1
    Accession Version XM_031900239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA
    ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT
    GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG
    GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT
    GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG
    GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC
    TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC
    GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT
    GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC
    CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG
    ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG
    CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG
    GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT
    CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT
    AACCTAAAAT TCATGCACAG TCAGGAGCGG AAGGTTTTCA TACAGCTGAA TCATATTAAA
    AAGTGTAACA CTGTACGGGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AACTAAAGAA
    GTGGTGAGCC ACAAATACAA GACACCCATG GCCCATGAGA TCTGCTACTC CGTCCTGTGT
    CTTTTCTCCT ACGTGGCCGC CATCAGAGGA AAGGAGGCAG AGCTGAAGAG TAGACCCCCT
    CGGCCTGTGT CAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756099.1
    CDS343..5220
    Translation

    Target ORF information:

    RefSeq Version XM_031900239.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA
    ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT
    GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG
    GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT
    GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG
    GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC
    TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC
    GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT
    GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC
    CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG
    ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG
    CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG
    GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT
    CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT
    AACCTAAAAT TCATGCACAG TCAGGAGCGG AAGGTTTTCA TACAGCTGAA TCATATTAAA
    AAGTGTAACA CTGTACGGGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AACTAAAGAA
    GTGGTGAGCC ACAAATACAA GACACCCATG GCCCATGAGA TCTGCTACTC CGTCCTGTGT
    CTTTTCTCCT ACGTGGCCGC CATCAGAGGA AAGGAGGCAG AGCTGAAGAG TAGACCCCCT
    CGGCCTGTGT CAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56210
    Clone ID Related Accession (Same CDS sequence) XM_012961380.3
    Accession Version XM_012961380.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816834.1
    CDS343..5214
    Translation

    Target ORF information:

    RefSeq Version XM_012961380.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961380.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56211
    Clone ID Related Accession (Same CDS sequence) XM_012961381.3
    Accession Version XM_012961381.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961381.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816835.1
    CDS343..5205
    Translation

    Target ORF information:

    RefSeq Version XM_012961381.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961381.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56212
    Clone ID Related Accession (Same CDS sequence) XM_031900240.1
    Accession Version XM_031900240.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756100.1
    CDS343..5202
    Translation

    Target ORF information:

    RefSeq Version XM_031900240.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900240.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56213
    Clone ID Related Accession (Same CDS sequence) XM_031900241.1
    Accession Version XM_031900241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA
    ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT
    GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG
    GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT
    GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG
    GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC
    TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC
    GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT
    GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC
    CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG
    ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG
    CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG
    GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT
    CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT
    AACCTAAAAT TCATGCACAG TCAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756101.1
    CDS343..5151
    Translation

    Target ORF information:

    RefSeq Version XM_031900241.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA
    ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT
    GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG
    GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT
    GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG
    GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC
    TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC
    GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT
    GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC
    CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG
    ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG
    CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG
    GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT
    CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT
    AACCTAAAAT TCATGCACAG TCAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56214
    Clone ID Related Accession (Same CDS sequence) XM_012961383.3
    Accession Version XM_012961383.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961383.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816837.1
    CDS343..5148
    Translation

    Target ORF information:

    RefSeq Version XM_012961383.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961383.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56215
    Clone ID Related Accession (Same CDS sequence) XM_031900242.1
    Accession Version XM_031900242.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756102.1
    CDS343..5148
    Translation

    Target ORF information:

    RefSeq Version XM_031900242.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900242.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56216
    Clone ID Related Accession (Same CDS sequence) XM_012961384.3
    Accession Version XM_012961384.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816838.1
    CDS343..5145
    Translation

    Target ORF information:

    RefSeq Version XM_012961384.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961384.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56217
    Clone ID Related Accession (Same CDS sequence) XM_012961386.3
    Accession Version XM_012961386.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816840.1
    CDS343..5142
    Translation

    Target ORF information:

    RefSeq Version XM_012961386.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961386.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56218
    Clone ID Related Accession (Same CDS sequence) XM_012961387.3
    Accession Version XM_012961387.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961387.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AGTTGTGGAA CACGCATCGG GAAGTGAAAA AGCAAAAAGC TATGGATAAA
    CAGAGTACCA AGCCAGCACC GCCTTTGGTT GATAGTGGGG AAACAGAGGA AAAGAAGTCC
    CAGATTAGCG CCGATAGTGG CCTGAGCGTC ACATCTGGTT CTCAGCGAAG CGATCTAGAC
    ACGGGCAGCA TAGGACCCTC CATCCTTATC CGAAGTAGCA GCCAGGATTC GGAAATCAGC
    ACAGTGGTGA GTAACAGCTC AGGGGAAACA CTGGGAGCCG ACAGCGACTT AAGTAGCAAC
    GCAGGGGATG CCTTGGGCGG CCCAGGAGGA AGCCACCTCT CGGGATCCAG AGGGGCTGTG
    TCCGACAGCG AGATTCAGAT GAACTCATTC ACCGGAGCCA TCTTTGGAAA GCCTCAGATG
    CTCCGCCCTG GTGAGAAAGA AAGGAATGTG AGTCCTGGGC GCCCCCAGCA AGACTTGAGC
    CAACGCATAT ACCTGTATGA GGGGCTTTTA GGAAGAGATA AGGGCTCAGT CTGGGACCAG
    CTGGAAGATG CAGCCATGGA AACCTTCTCA TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC
    CAGATGCAGT TTTGGGAGGA TGCTTTCCTA GACGCTGTTA TGCTGGAGAG AGAAGGAATG
    GGTATGGACC AAGGCCCACA GGAGATGATA GACAGGTATT TCTCTCTTGG CGACCATGAC
    CGGAAGCGCT TAGAGGATGA TGAGGATCGC CTGCTGGCCA CTCTTTTGCA CAATATGATT
    GCCTATATGA TCATGATGAA GGTGAATAAG ACAGATCTAC GCAGGAAGGT CAGGCGTATC
    CTTGGAAAGT GCCACATAGG CTTAGTGCAC AGCCAACAAA TTAATGAACT GCTGGACAGG
    GTGACTGACA TGAATGGCCG CGATCTCAGT ATTCGTCCCA GTGGGAGCCG TCACATCAAG
    AAGCAAACCT TTGTAGTGCA TGCTGGGACA GACACAAGTG GGGACCTTTT CTTTATGGAG
    GTTTGTGACG ACTGCATCGT CCTTCGCAGC AACATCGGTG CCGTGTATGA ACGTTGGTGG
    TACGAAAAAC TTATTAACAT GACTTATTGT CCCAAGACTA AAGTGCTCTG TCTGTGGAGG
    AGAAATGGCC AAGAAACTCA GCTCAACAAA TTCTTCACCA AGAAGTGCCG GGAGCTGTAC
    TACTGTGTGA AGGAGAGCAT GGAACGGGCG GCAGCACGAC AGCAGAGCAT AAAGCCAGGG
    CCGGAACTTG GCGGAGAGTT TCCTGTCCAG GACATGAAGA CAGGAGAAGG GGGTCTTCTT
    CAAGTTACCT TGGAGGGAAT TAACCTAAAA TTCATGCACA GTCAGGAGCG GAAGGTTTTC
    ATACAGCTGA ATCATATTAA AAAGTGTAAC ACTGTACGGG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAACTAAAGA AGTGGTGAGC CACAAATACA AGACACCCAT GGCCCATGAG
    ATCTGCTACT CCGTCCTGTG TCTTTTCTCC TACGTGGCCG CCATCAGAGG AAAGGAGGCA
    GAGCTGAAGA GTAGACCCCC TCGGCCTGTG TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816841.1
    CDS343..5121
    Translation

    Target ORF information:

    RefSeq Version XM_012961387.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961387.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AGTTGTGGAA CACGCATCGG GAAGTGAAAA AGCAAAAAGC TATGGATAAA
    CAGAGTACCA AGCCAGCACC GCCTTTGGTT GATAGTGGGG AAACAGAGGA AAAGAAGTCC
    CAGATTAGCG CCGATAGTGG CCTGAGCGTC ACATCTGGTT CTCAGCGAAG CGATCTAGAC
    ACGGGCAGCA TAGGACCCTC CATCCTTATC CGAAGTAGCA GCCAGGATTC GGAAATCAGC
    ACAGTGGTGA GTAACAGCTC AGGGGAAACA CTGGGAGCCG ACAGCGACTT AAGTAGCAAC
    GCAGGGGATG CCTTGGGCGG CCCAGGAGGA AGCCACCTCT CGGGATCCAG AGGGGCTGTG
    TCCGACAGCG AGATTCAGAT GAACTCATTC ACCGGAGCCA TCTTTGGAAA GCCTCAGATG
    CTCCGCCCTG GTGAGAAAGA AAGGAATGTG AGTCCTGGGC GCCCCCAGCA AGACTTGAGC
    CAACGCATAT ACCTGTATGA GGGGCTTTTA GGAAGAGATA AGGGCTCAGT CTGGGACCAG
    CTGGAAGATG CAGCCATGGA AACCTTCTCA TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC
    CAGATGCAGT TTTGGGAGGA TGCTTTCCTA GACGCTGTTA TGCTGGAGAG AGAAGGAATG
    GGTATGGACC AAGGCCCACA GGAGATGATA GACAGGTATT TCTCTCTTGG CGACCATGAC
    CGGAAGCGCT TAGAGGATGA TGAGGATCGC CTGCTGGCCA CTCTTTTGCA CAATATGATT
    GCCTATATGA TCATGATGAA GGTGAATAAG ACAGATCTAC GCAGGAAGGT CAGGCGTATC
    CTTGGAAAGT GCCACATAGG CTTAGTGCAC AGCCAACAAA TTAATGAACT GCTGGACAGG
    GTGACTGACA TGAATGGCCG CGATCTCAGT ATTCGTCCCA GTGGGAGCCG TCACATCAAG
    AAGCAAACCT TTGTAGTGCA TGCTGGGACA GACACAAGTG GGGACCTTTT CTTTATGGAG
    GTTTGTGACG ACTGCATCGT CCTTCGCAGC AACATCGGTG CCGTGTATGA ACGTTGGTGG
    TACGAAAAAC TTATTAACAT GACTTATTGT CCCAAGACTA AAGTGCTCTG TCTGTGGAGG
    AGAAATGGCC AAGAAACTCA GCTCAACAAA TTCTTCACCA AGAAGTGCCG GGAGCTGTAC
    TACTGTGTGA AGGAGAGCAT GGAACGGGCG GCAGCACGAC AGCAGAGCAT AAAGCCAGGG
    CCGGAACTTG GCGGAGAGTT TCCTGTCCAG GACATGAAGA CAGGAGAAGG GGGTCTTCTT
    CAAGTTACCT TGGAGGGAAT TAACCTAAAA TTCATGCACA GTCAGGAGCG GAAGGTTTTC
    ATACAGCTGA ATCATATTAA AAAGTGTAAC ACTGTACGGG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAACTAAAGA AGTGGTGAGC CACAAATACA AGACACCCAT GGCCCATGAG
    ATCTGCTACT CCGTCCTGTG TCTTTTCTCC TACGTGGCCG CCATCAGAGG AAAGGAGGCA
    GAGCTGAAGA GTAGACCCCC TCGGCCTGTG TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56219
    Clone ID Related Accession (Same CDS sequence) XM_012961388.3
    Accession Version XM_012961388.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961388.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816842.1
    CDS343..5121
    Translation

    Target ORF information:

    RefSeq Version XM_012961388.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961388.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56220
    Clone ID Related Accession (Same CDS sequence) XM_031900243.1
    Accession Version XM_031900243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGTTCCTGAA ACTAAAGAAG TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756103.1
    CDS343..5118
    Translation

    Target ORF information:

    RefSeq Version XM_031900243.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGTTCCTGAA ACTAAAGAAG TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56221
    Clone ID Related Accession (Same CDS sequence) XM_012961390.3
    Accession Version XM_012961390.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816844.1
    CDS343..5088
    Translation

    Target ORF information:

    RefSeq Version XM_012961390.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961390.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56222
    Clone ID Related Accession (Same CDS sequence) XM_012961391.3
    Accession Version XM_012961391.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961391.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816845.1
    CDS343..5085
    Translation

    Target ORF information:

    RefSeq Version XM_012961391.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961391.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56223
    Clone ID Related Accession (Same CDS sequence) XM_031900244.1
    Accession Version XM_031900244.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756104.1
    CDS343..5067
    Translation

    Target ORF information:

    RefSeq Version XM_031900244.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56224
    Clone ID Related Accession (Same CDS sequence) XM_012961393.3
    Accession Version XM_012961393.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961393.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816847.1
    CDS343..5064
    Translation

    Target ORF information:

    RefSeq Version XM_012961393.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961393.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56225
    Clone ID Related Accession (Same CDS sequence) XM_031900245.1
    Accession Version XM_031900245.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756105.1
    CDS343..5064
    Translation

    Target ORF information:

    RefSeq Version XM_031900245.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56226
    Clone ID Related Accession (Same CDS sequence) XM_031900246.1
    Accession Version XM_031900246.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X33
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756106.1
    CDS343..5061
    Translation

    Target ORF information:

    RefSeq Version XM_031900246.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56227
    Clone ID Related Accession (Same CDS sequence) XM_012961394.3
    Accession Version XM_012961394.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816848.1
    CDS343..5061
    Translation

    Target ORF information:

    RefSeq Version XM_012961394.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961394.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56228
    Clone ID Related Accession (Same CDS sequence) XM_031900247.1
    Accession Version XM_031900247.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGTTCCTGAA ACTAAAGAAG TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756107.1
    CDS343..5058
    Translation

    Target ORF information:

    RefSeq Version XM_031900247.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X36, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGTTCCTGAA ACTAAAGAAG TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56229
    Clone ID Related Accession (Same CDS sequence) XM_031900248.1
    Accession Version XM_031900248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA
    ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT
    GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG
    GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT
    GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG
    GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC
    TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC
    GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT
    GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC
    CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG
    ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG
    CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG
    GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT
    CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT
    AACCTAAAAT TCATGCACAG TCAGTTCCTG AAACTAAAGA AGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756108.1
    CDS343..5010
    Translation

    Target ORF information:

    RefSeq Version XM_031900248.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X37, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA
    ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT
    GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG
    GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT
    GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG
    GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC
    TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC
    GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT
    GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC
    CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG
    ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG
    CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG
    GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT
    CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT
    AACCTAAAAT TCATGCACAG TCAGTTCCTG AAACTAAAGA AGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56230
    Clone ID Related Accession (Same CDS sequence) XM_031900249.1
    Accession Version XM_031900249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756109.1
    CDS343..5007
    Translation

    Target ORF information:

    RefSeq Version XM_031900249.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56231
    Clone ID Related Accession (Same CDS sequence) XM_031900250.1
    Accession Version XM_031900250.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756110.1
    CDS343..5007
    Translation

    Target ORF information:

    RefSeq Version XM_031900250.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X39, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900250.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56232
    Clone ID Related Accession (Same CDS sequence) XM_012961397.3
    Accession Version XM_012961397.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X39
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816851.1
    CDS343..5004
    Translation

    Target ORF information:

    RefSeq Version XM_012961397.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961397.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56233
    Clone ID Related Accession (Same CDS sequence) XM_031900251.1
    Accession Version XM_031900251.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X40
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756111.1
    CDS343..5004
    Translation

    Target ORF information:

    RefSeq Version XM_031900251.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X41, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900251.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56234
    Clone ID Related Accession (Same CDS sequence) XM_031900252.1
    Accession Version XM_031900252.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4659bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X41
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756112.1
    CDS343..5001
    Translation

    Target ORF information:

    RefSeq Version XM_031900252.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900252.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56235
    Clone ID Related Accession (Same CDS sequence) XM_031900253.1
    Accession Version XM_031900253.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X42
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756113.1
    CDS343..4947
    Translation

    Target ORF information:

    RefSeq Version XM_031900253.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X43, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900253.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56236
    Clone ID Related Accession (Same CDS sequence) XM_012961398.3
    Accession Version XM_012961398.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X43
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816852.1
    CDS343..4944
    Translation

    Target ORF information:

    RefSeq Version XM_012961398.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X44, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961398.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56237
    Clone ID Related Accession (Same CDS sequence) XM_031900254.1
    Accession Version XM_031900254.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X44
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756114.1
    CDS343..5202
    Translation

    Target ORF information:

    RefSeq Version XM_031900254.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X45, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900254.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56238
    Clone ID Related Accession (Same CDS sequence) XM_031900255.1
    Accession Version XM_031900255.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X45
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756115.1
    CDS343..5145
    Translation

    Target ORF information:

    RefSeq Version XM_031900255.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X46, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900255.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56239
    Clone ID Related Accession (Same CDS sequence) XM_031900256.1
    Accession Version XM_031900256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X46
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756116.1
    CDS343..5208
    Translation

    Target ORF information:

    RefSeq Version XM_031900256.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X47, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56240
    Clone ID Related Accession (Same CDS sequence) XM_031900257.1
    Accession Version XM_031900257.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X47
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA
    ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT
    GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG
    GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT
    GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG
    GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC
    TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC
    GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT
    GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC
    CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG
    ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG
    CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG
    GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT
    CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT
    AACCTAAAAT TCATGCACAG TCAGTTCCTG AAACTAAAGA AGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756117.1
    CDS343..4950
    Translation

    Target ORF information:

    RefSeq Version XM_031900257.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X48, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA
    ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT
    GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG
    GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT
    GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG
    GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC
    TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC
    GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT
    GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC
    CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG
    ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG
    CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG
    GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT
    CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT
    AACCTAAAAT TCATGCACAG TCAGTTCCTG AAACTAAAGA AGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56241
    Clone ID Related Accession (Same CDS sequence) XM_018093105.2
    Accession Version XM_018093105.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X48
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948594.1
    CDS343..4887
    Translation

    Target ORF information:

    RefSeq Version XM_018093105.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X49, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093105.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22139
    Clone ID Related Accession (Same CDS sequence) NM_001079249.1
    Accession Version NM_001079249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125676.1. ##Evidence-Data-START## Transcript exon combination :: BC125676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028292 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG
    AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT
    CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA
    ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT
    AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG
    GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT
    CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC
    TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGTA AGGAGCGTTC CACTCTGTGG
    GACCAGATGC AGTTTTGGGA GGATGCTTTC CTAGACGCTG TTATGCTGGA GAGAGAAGGA
    ATGGGTATGG ACCAAGGCCC ACAGGAGATG ATAGACAGGT ATTTCTCTCT TGGCGACCAT
    GACCGGAAGC GCTTAGAGGA TGATGAGGAT CGCCTGCTGG CCACTCTTTT GCACAATATG
    ATTGCCTATA TGATCATGAT GAAGGTGAAT AAGACAGATC TACGCAGGAA GGTCAGGCGT
    ATCCTTGGAA AGTGCCACAT AGGCTTAGTG CACAGCCAAC AAATTAATGA ACTGCTGGAC
    AGGGTGACTG ACATGAATGG CCGCGATCTC AGTATTCGTC CCAGTGGGAG CCGTCACATC
    AAGAAGCAAA CCTTTGTAGT GCATGCTGGG ACAGACACAA GTGGGGACCT TTTCTTTATG
    GAGGTTTGTG ACGACTGCAT CGTCCTTCGC AGCAACATCG GTGCCGTGTA TGAACGTTGG
    TGGTACGAAA AACTTATTAA CATGACTTAT TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG
    AGGAGAAATG GCCAAGAAAC TCAGCTCAAC AAATTCTTCA CCAAGAAGTG CCGGGAGCTG
    TACTACTGTG TGAAGGAGAG CATGGAACGG GCGGCAGCAC GACAGCAGAG CATAAAGCCA
    GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC CAGGACATGA AGACAGGAGA AGGGGGTCTT
    CTTCAAGTTA CCTTGGAGGG AATTAACCTA AAATTCATGC ACAGTCAGGT TTTCATACAG
    CTGAATCATA TTAAAAAGTG TAACACTGTA CGGGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAACTA AAGAAGTGGT GAGCCACAAA TACAAGACAC CCATGGCCCA TGAGATCTGC
    TACTCCGTCC TGTGTCTTTT CTCCTACGTG GCCGCCATCA GAGGAAAGGA GGCAGAGCTG
    AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072717.1
    CDS95..4687
    Translation

    Target ORF information:

    RefSeq Version NM_001079249.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG
    AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT
    CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA
    ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT
    AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG
    GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT
    CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC
    TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGTA AGGAGCGTTC CACTCTGTGG
    GACCAGATGC AGTTTTGGGA GGATGCTTTC CTAGACGCTG TTATGCTGGA GAGAGAAGGA
    ATGGGTATGG ACCAAGGCCC ACAGGAGATG ATAGACAGGT ATTTCTCTCT TGGCGACCAT
    GACCGGAAGC GCTTAGAGGA TGATGAGGAT CGCCTGCTGG CCACTCTTTT GCACAATATG
    ATTGCCTATA TGATCATGAT GAAGGTGAAT AAGACAGATC TACGCAGGAA GGTCAGGCGT
    ATCCTTGGAA AGTGCCACAT AGGCTTAGTG CACAGCCAAC AAATTAATGA ACTGCTGGAC
    AGGGTGACTG ACATGAATGG CCGCGATCTC AGTATTCGTC CCAGTGGGAG CCGTCACATC
    AAGAAGCAAA CCTTTGTAGT GCATGCTGGG ACAGACACAA GTGGGGACCT TTTCTTTATG
    GAGGTTTGTG ACGACTGCAT CGTCCTTCGC AGCAACATCG GTGCCGTGTA TGAACGTTGG
    TGGTACGAAA AACTTATTAA CATGACTTAT TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG
    AGGAGAAATG GCCAAGAAAC TCAGCTCAAC AAATTCTTCA CCAAGAAGTG CCGGGAGCTG
    TACTACTGTG TGAAGGAGAG CATGGAACGG GCGGCAGCAC GACAGCAGAG CATAAAGCCA
    GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC CAGGACATGA AGACAGGAGA AGGGGGTCTT
    CTTCAAGTTA CCTTGGAGGG AATTAACCTA AAATTCATGC ACAGTCAGGT TTTCATACAG
    CTGAATCATA TTAAAAAGTG TAACACTGTA CGGGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAACTA AAGAAGTGGT GAGCCACAAA TACAAGACAC CCATGGCCCA TGAGATCTGC
    TACTCCGTCC TGTGTCTTTT CTCCTACGTG GCCGCCATCA GAGGAAAGGA GGCAGAGCTG
    AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29527
    Clone ID Related Accession (Same CDS sequence) XM_012961370.2 , XM_012961368.2
    Accession Version XM_012961370.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961370.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816824.1
    CDS816..5804
    Translation

    Target ORF information:

    RefSeq Version XM_012961370.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961370.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29527
    Clone ID Related Accession (Same CDS sequence) XM_012961370.2 , XM_012961368.2
    Accession Version XM_012961368.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816822.1
    CDS175..5163
    Translation

    Target ORF information:

    RefSeq Version XM_012961368.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961368.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29528
    Clone ID Related Accession (Same CDS sequence) XM_012961371.2
    Accession Version XM_012961371.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGCATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816825.1
    CDS176..5161
    Translation

    Target ORF information:

    RefSeq Version XM_012961371.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961371.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG
    GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC
    CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC
    GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT
    CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA
    AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG
    AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGCATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29529
    Clone ID Related Accession (Same CDS sequence) XM_012961372.2
    Accession Version XM_012961372.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816826.1
    CDS175..5154
    Translation

    Target ORF information:

    RefSeq Version XM_012961372.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961372.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29530
    Clone ID Related Accession (Same CDS sequence) XM_012961373.2
    Accession Version XM_012961373.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816827.1
    CDS175..5109
    Translation

    Target ORF information:

    RefSeq Version XM_012961373.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961373.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29531
    Clone ID Related Accession (Same CDS sequence) XM_012961375.2
    Accession Version XM_012961375.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816829.1
    CDS175..5106
    Translation

    Target ORF information:

    RefSeq Version XM_012961375.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961375.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29532
    Clone ID Related Accession (Same CDS sequence) XM_012961376.2
    Accession Version XM_012961376.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816830.1
    CDS175..5103
    Translation

    Target ORF information:

    RefSeq Version XM_012961376.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961376.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC
    ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC
    CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC
    TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG
    TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29533
    Clone ID Related Accession (Same CDS sequence) XM_012961377.2
    Accession Version XM_012961377.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGCATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816831.1
    CDS175..5100
    Translation

    Target ORF information:

    RefSeq Version XM_012961377.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961377.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC
    GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC
    AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG
    CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA
    GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC
    CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGCATT
    CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC
    ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC
    ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC
    AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC
    TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA
    GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC
    ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC
    ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29534
    Clone ID Related Accession (Same CDS sequence) XM_012961378.2
    Accession Version XM_012961378.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816832.1
    CDS175..5097
    Translation

    Target ORF information:

    RefSeq Version XM_012961378.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961378.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29535
    Clone ID Related Accession (Same CDS sequence) XM_012961379.2
    Accession Version XM_012961379.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816833.1
    CDS175..5049
    Translation

    Target ORF information:

    RefSeq Version XM_012961379.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961379.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC
    TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29536
    Clone ID Related Accession (Same CDS sequence) XM_012961380.2
    Accession Version XM_012961380.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816834.1
    CDS175..5046
    Translation

    Target ORF information:

    RefSeq Version XM_012961380.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961380.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT
    AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG
    AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC
    TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT
    GTGTCAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29537
    Clone ID Related Accession (Same CDS sequence) XM_012961381.2
    Accession Version XM_012961381.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816835.1
    CDS175..5037
    Translation

    Target ORF information:

    RefSeq Version XM_012961381.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961381.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29538
    Clone ID Related Accession (Same CDS sequence) XM_012961382.2
    Accession Version XM_012961382.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816836.1
    CDS175..4989
    Translation

    Target ORF information:

    RefSeq Version XM_012961382.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961382.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29539
    Clone ID Related Accession (Same CDS sequence) XM_012961383.2
    Accession Version XM_012961383.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816837.1
    CDS174..4979
    Translation

    Target ORF information:

    RefSeq Version XM_012961383.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961383.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29540
    Clone ID Related Accession (Same CDS sequence) XM_012961384.2
    Accession Version XM_012961384.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816838.1
    CDS174..4976
    Translation

    Target ORF information:

    RefSeq Version XM_012961384.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961384.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29541
    Clone ID Related Accession (Same CDS sequence) XM_012961386.2
    Accession Version XM_012961386.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816840.1
    CDS174..4973
    Translation

    Target ORF information:

    RefSeq Version XM_012961386.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961386.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG
    GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC
    AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC
    GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29542
    Clone ID Related Accession (Same CDS sequence) XM_012961387.2
    Accession Version XM_012961387.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AGTTGTGGAA CACGCATCGG GAAGTGAAAA AGCAAAAAGC TATGGATAAA
    CAGAGTACCA AGCCAGCACC GCCTTTGGTT GATAGTGGGG AAACAGAGGA AAAGAAGTCC
    CAGATTAGCG CCGATAGTGG CCTGAGCGTC ACATCTGGTT CTCAGCGAAG CGATCTAGAC
    ACAGGCAGCA TAGGACCCTC CATCCTTATC CGAAGTAGCA GCCAGGATTC GGAAATCAGC
    ACAGTGGTGA GTAACAGCTC AGGGGAAACA CTGGGAGCCG ACAGCGACTT AAGTAGCAAC
    GCAGGGGATG CCTTGGGCGG CCCAGGAGGA AGCCACCTCT CGGGATCCAG AGGGGCTGTG
    TCCGACAGCG AGATTCAGAT GAACTCATTC ACCGGAGCCA TCTTTGGAAA GCCTCAGATG
    CTCCGCCCTG GTGAGAAAGA AAGGAATGTG AGTCCTGGGC GCCCCCAGCA AGACTTGAGC
    CAACGCATAT ACCTGTATGA GGGGCTTTTA GGAAGAGATA AGGGCTCAGT CTGGGACCAG
    CTGGAAGATG CAGCCATGGA AACCTTCTCA TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC
    CAGATGCAGT TTTGGGAGGA TGCTTTCCTA GACGCTGTTA TGCTGGAGAG AGAAGGAATG
    GGTATGGACC AAGGCCCACA GGAGATGATA GACAGGTATT TCTCTCTTGG CGACCATGAC
    CGGAAGCGCT TAGAGGATGA TGAGGATCGC CTGCTGGCCA CTCTTTTGCA CAATATGATT
    GCCTATATGA TCATGATGAA GGTGAATAAG ACAGATCTAC GCAGGAAGGT CAGGCGTATC
    CTTGGAAAGT GCCACATAGG CTTAGTGCAC AGCCAACAAA TTAATGAACT GCTGGACAGG
    GTGACTGACA TGAATGGCCG CGATCTCAGC ATTCGTCCCA GTGGGAGCCG TCACATCAAG
    AAGCAAACCT TTGTAGTGCA TGCTGGGACA GACACAAGTG GGGACCTTTT CTTTATGGAG
    GTTTGTGACG ACTGCATCGT CCTTCGCAGC AACATCGGTG CCGTGTATGA ACGTTGGTGG
    TACGAAAAAC TTATTAACAT GACTTATTGT CCCAAGACTA AAGTGCTCTG TCTGTGGAGG
    AGAAATGGCC AAGAAACTCA GCTCAACAAA TTCTTCACCA AGAAGTGCCG GGAGCTGTAC
    TACTGTGTGA AGGAGAGCAT GGAACGGGCG GCAGCACGAC AGCAGAGCAT AAAGCCAGGG
    CCGGAACTTG GCGGAGAGTT TCCTGTCCAG GACATGAAGA CAGGAGAAGG GGGTCTTCTT
    CAAGTTACCT TGGAGGGAAT TAACCTAAAA TTCATGCACA GTCAGGAGCG GAAGGTTTTC
    ATACAGCTGA ATCATATTAA AAAGTGTAAC ACTGTACGGG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAACTAAAGA AGTGGTGAGC CACAAATACA AGACACCCAT GGCCCATGAG
    ATCTGCTACT CCGTCCTGTG TCTTTTCTCC TACGTGGCCG CCATCAGAGG AAAGGAGGCA
    GAGCTGAAGA GTAGACCCCC TCGGCCTGTG TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816841.1
    CDS175..4953
    Translation

    Target ORF information:

    RefSeq Version XM_012961387.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961387.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AGTTGTGGAA CACGCATCGG GAAGTGAAAA AGCAAAAAGC TATGGATAAA
    CAGAGTACCA AGCCAGCACC GCCTTTGGTT GATAGTGGGG AAACAGAGGA AAAGAAGTCC
    CAGATTAGCG CCGATAGTGG CCTGAGCGTC ACATCTGGTT CTCAGCGAAG CGATCTAGAC
    ACAGGCAGCA TAGGACCCTC CATCCTTATC CGAAGTAGCA GCCAGGATTC GGAAATCAGC
    ACAGTGGTGA GTAACAGCTC AGGGGAAACA CTGGGAGCCG ACAGCGACTT AAGTAGCAAC
    GCAGGGGATG CCTTGGGCGG CCCAGGAGGA AGCCACCTCT CGGGATCCAG AGGGGCTGTG
    TCCGACAGCG AGATTCAGAT GAACTCATTC ACCGGAGCCA TCTTTGGAAA GCCTCAGATG
    CTCCGCCCTG GTGAGAAAGA AAGGAATGTG AGTCCTGGGC GCCCCCAGCA AGACTTGAGC
    CAACGCATAT ACCTGTATGA GGGGCTTTTA GGAAGAGATA AGGGCTCAGT CTGGGACCAG
    CTGGAAGATG CAGCCATGGA AACCTTCTCA TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC
    CAGATGCAGT TTTGGGAGGA TGCTTTCCTA GACGCTGTTA TGCTGGAGAG AGAAGGAATG
    GGTATGGACC AAGGCCCACA GGAGATGATA GACAGGTATT TCTCTCTTGG CGACCATGAC
    CGGAAGCGCT TAGAGGATGA TGAGGATCGC CTGCTGGCCA CTCTTTTGCA CAATATGATT
    GCCTATATGA TCATGATGAA GGTGAATAAG ACAGATCTAC GCAGGAAGGT CAGGCGTATC
    CTTGGAAAGT GCCACATAGG CTTAGTGCAC AGCCAACAAA TTAATGAACT GCTGGACAGG
    GTGACTGACA TGAATGGCCG CGATCTCAGC ATTCGTCCCA GTGGGAGCCG TCACATCAAG
    AAGCAAACCT TTGTAGTGCA TGCTGGGACA GACACAAGTG GGGACCTTTT CTTTATGGAG
    GTTTGTGACG ACTGCATCGT CCTTCGCAGC AACATCGGTG CCGTGTATGA ACGTTGGTGG
    TACGAAAAAC TTATTAACAT GACTTATTGT CCCAAGACTA AAGTGCTCTG TCTGTGGAGG
    AGAAATGGCC AAGAAACTCA GCTCAACAAA TTCTTCACCA AGAAGTGCCG GGAGCTGTAC
    TACTGTGTGA AGGAGAGCAT GGAACGGGCG GCAGCACGAC AGCAGAGCAT AAAGCCAGGG
    CCGGAACTTG GCGGAGAGTT TCCTGTCCAG GACATGAAGA CAGGAGAAGG GGGTCTTCTT
    CAAGTTACCT TGGAGGGAAT TAACCTAAAA TTCATGCACA GTCAGGAGCG GAAGGTTTTC
    ATACAGCTGA ATCATATTAA AAAGTGTAAC ACTGTACGGG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAACTAAAGA AGTGGTGAGC CACAAATACA AGACACCCAT GGCCCATGAG
    ATCTGCTACT CCGTCCTGTG TCTTTTCTCC TACGTGGCCG CCATCAGAGG AAAGGAGGCA
    GAGCTGAAGA GTAGACCCCC TCGGCCTGTG TCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29543
    Clone ID Related Accession (Same CDS sequence) XM_012961388.2
    Accession Version XM_012961388.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816842.1
    CDS176..4954
    Translation

    Target ORF information:

    RefSeq Version XM_012961388.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961388.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG
    GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT
    GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC
    ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC
    CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29544
    Clone ID Related Accession (Same CDS sequence) XM_012961389.2
    Accession Version XM_012961389.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816843.1
    CDS174..4928
    Translation

    Target ORF information:

    RefSeq Version XM_012961389.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961389.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG
    TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG
    GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT
    TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG
    CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29545
    Clone ID Related Accession (Same CDS sequence) XM_012961390.2
    Accession Version XM_012961390.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816844.1
    CDS174..4919
    Translation

    Target ORF information:

    RefSeq Version XM_012961390.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961390.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT
    GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC
    AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC
    GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29546
    Clone ID Related Accession (Same CDS sequence) XM_012961391.2
    Accession Version XM_012961391.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816845.1
    CDS174..4916
    Translation

    Target ORF information:

    RefSeq Version XM_012961391.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961391.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA
    CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA
    TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG
    GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29547
    Clone ID Related Accession (Same CDS sequence) XM_012961392.2
    Accession Version XM_012961392.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG
    AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT
    CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA
    ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT
    AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG
    GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT
    CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC
    TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGAA GAGATAAGGG CTCAGTCTGG
    GACCAGCTGG AAGATGCAGC CATGGAAACC TTCTCATTGA GTAAGGAGCG TTCCACTCTG
    TGGGACCAGA TGCAGTTTTG GGAGGATGCT TTCCTAGACG CTGTTATGCT GGAGAGAGAA
    GGAATGGGTA TGGACCAAGG CCCACAGGAG ATGATAGACA GGTATTTCTC TCTTGGCGAC
    CATGACCGGA AGCGCTTAGA GGATGATGAG GATCGCCTGC TGGCCACTCT TTTGCACAAT
    ATGATTGCCT ATATGATCAT GATGAAGGTG AATAAGACAG ATCTACGCAG GAAGGTCAGG
    CGTATCCTTG GAAAGTGCCA CATAGGCTTA GTGCACAGCC AACAAATTAA TGAACTGCTG
    GACAGGGTGA CTGACATGAA TGGCCGCGAT CTCAGCATTC GTCCCAGTGG GAGCCGTCAC
    ATCAAGAAGC AAACCTTTGT AGTGCATGCT GGGACAGACA CAAGTGGGGA CCTTTTCTTT
    ATGGAGGTTT GTGACGACTG CATCGTCCTT CGCAGCAACA TCGGTGCCGT GTATGAACGT
    TGGTGGTACG AAAAACTTAT TAACATGACT TATTGTCCCA AGACTAAAGT GCTCTGTCTG
    TGGAGGAGAA ATGGCCAAGA AACTCAGCTC AACAAATTCT TCACCAAGAA GTGCCGGGAG
    CTGTACTACT GTGTGAAGGA GAGCATGGAA CGGGCGGCAG CACGACAGCA GAGCATAAAG
    CCAGGGCCGG AACTTGGCGG AGAGTTTCCT GTCCAGGACA TGAAGACAGG AGAAGGGGGT
    CTTCTTCAAG TTACCTTGGA GGGAATTAAC CTAAAATTCA TGCACAGTCA GGAGCGGAAG
    GTTTTCATAC AGCTGAATCA TATTAAAAAG TGTAACACTG TACGGGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAC TAAAGAAGTG GTGAGCCACA AATACAAGAC ACCCATGGCC
    CATGAGATCT GCTACTCCGT CCTGTGTCTT TTCTCCTACG TGGCCGCCAT CAGAGGAAAG
    GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816846.1
    CDS175..4899
    Translation

    Target ORF information:

    RefSeq Version XM_012961392.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961392.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT
    ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG
    AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT
    CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA
    ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT
    AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG
    GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT
    CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC
    TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGAA GAGATAAGGG CTCAGTCTGG
    GACCAGCTGG AAGATGCAGC CATGGAAACC TTCTCATTGA GTAAGGAGCG TTCCACTCTG
    TGGGACCAGA TGCAGTTTTG GGAGGATGCT TTCCTAGACG CTGTTATGCT GGAGAGAGAA
    GGAATGGGTA TGGACCAAGG CCCACAGGAG ATGATAGACA GGTATTTCTC TCTTGGCGAC
    CATGACCGGA AGCGCTTAGA GGATGATGAG GATCGCCTGC TGGCCACTCT TTTGCACAAT
    ATGATTGCCT ATATGATCAT GATGAAGGTG AATAAGACAG ATCTACGCAG GAAGGTCAGG
    CGTATCCTTG GAAAGTGCCA CATAGGCTTA GTGCACAGCC AACAAATTAA TGAACTGCTG
    GACAGGGTGA CTGACATGAA TGGCCGCGAT CTCAGCATTC GTCCCAGTGG GAGCCGTCAC
    ATCAAGAAGC AAACCTTTGT AGTGCATGCT GGGACAGACA CAAGTGGGGA CCTTTTCTTT
    ATGGAGGTTT GTGACGACTG CATCGTCCTT CGCAGCAACA TCGGTGCCGT GTATGAACGT
    TGGTGGTACG AAAAACTTAT TAACATGACT TATTGTCCCA AGACTAAAGT GCTCTGTCTG
    TGGAGGAGAA ATGGCCAAGA AACTCAGCTC AACAAATTCT TCACCAAGAA GTGCCGGGAG
    CTGTACTACT GTGTGAAGGA GAGCATGGAA CGGGCGGCAG CACGACAGCA GAGCATAAAG
    CCAGGGCCGG AACTTGGCGG AGAGTTTCCT GTCCAGGACA TGAAGACAGG AGAAGGGGGT
    CTTCTTCAAG TTACCTTGGA GGGAATTAAC CTAAAATTCA TGCACAGTCA GGAGCGGAAG
    GTTTTCATAC AGCTGAATCA TATTAAAAAG TGTAACACTG TACGGGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAC TAAAGAAGTG GTGAGCCACA AATACAAGAC ACCCATGGCC
    CATGAGATCT GCTACTCCGT CCTGTGTCTT TTCTCCTACG TGGCCGCCAT CAGAGGAAAG
    GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29548
    Clone ID Related Accession (Same CDS sequence) XM_012961393.2
    Accession Version XM_012961393.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816847.1
    CDS175..4896
    Translation

    Target ORF information:

    RefSeq Version XM_012961393.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961393.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29549
    Clone ID Related Accession (Same CDS sequence) XM_012961394.2
    Accession Version XM_012961394.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816848.1
    CDS176..4894
    Translation

    Target ORF information:

    RefSeq Version XM_012961394.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961394.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT
    AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA
    CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA
    AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC
    ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG
    AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA
    GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA
    TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA
    GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA
    GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC
    CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG
    ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC
    AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC
    ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA
    GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC
    AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT
    CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA
    TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG
    GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG
    GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA
    TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29550
    Clone ID Related Accession (Same CDS sequence) XM_012961395.2
    Accession Version XM_012961395.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG
    AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT
    CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA
    ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT
    AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG
    GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT
    CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC
    TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGAA GAGATAAGGG CTCAGTCTGG
    GACCAGCTGG AAGATGCAGC CATGGAAACC TTCTCATTGA GTAAGGAGCG TTCCACTCTG
    TGGGACCAGA TGCAGTTTTG GGAGGATGCT TTCCTAGACG CTGTTATGCT GGAGAGAGAA
    GGAATGGGTA TGGACCAAGG CCCACAGGAG ATGATAGACA GGTATTTCTC TCTTGGCGAC
    CATGACCGGA AGCGCTTAGA GGATGATGAG GATCGCCTGC TGGCCACTCT TTTGCACAAT
    ATGATTGCCT ATATGATCAT GATGAAGGTG AATAAGACAG ATCTACGCAG GAAGGTCAGG
    CGTATCCTTG GAAAGTGCCA CATAGGCTTA GTGCACAGCC AACAAATTAA TGAACTGCTG
    GACAGGGTGA CTGACATGAA TGGCCGCGAT CTCAGCATTC GTCCCAGTGG GAGCCGTCAC
    ATCAAGAAGC AAACCTTTGT AGTGCATGCT GGGACAGACA CAAGTGGGGA CCTTTTCTTT
    ATGGAGGTTT GTGACGACTG CATCGTCCTT CGCAGCAACA TCGGTGCCGT GTATGAACGT
    TGGTGGTACG AAAAACTTAT TAACATGACT TATTGTCCCA AGACTAAAGT GCTCTGTCTG
    TGGAGGAGAA ATGGCCAAGA AACTCAGCTC AACAAATTCT TCACCAAGAA GTGCCGGGAG
    CTGTACTACT GTGTGAAGGA GAGCATGGAA CGGGCGGCAG CACGACAGCA GAGCATAAAG
    CCAGGGCCGG AACTTGGCGG AGAGTTTCCT GTCCAGGACA TGAAGACAGG AGAAGGGGGT
    CTTCTTCAAG TTACCTTGGA GGGAATTAAC CTAAAATTCA TGCACAGTCA GGAGCGGAAG
    GTTTTCATAC AGCTGAATCA TATTAAAAAG TGTAACACTG TACGGGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAC TAAAGAAGTG GTGAGCCACA AATACAAGAC ACCCATGGCC
    CATGAGATCT GCTACTCCGT CCTGTGTCTT TTCTCCTACG TGGCCGCCAT CAGAGGAAAG
    GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816849.1
    CDS174..4838
    Translation

    Target ORF information:

    RefSeq Version XM_012961395.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961395.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG
    AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT
    CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA
    ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT
    AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG
    GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT
    CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC
    TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGAA GAGATAAGGG CTCAGTCTGG
    GACCAGCTGG AAGATGCAGC CATGGAAACC TTCTCATTGA GTAAGGAGCG TTCCACTCTG
    TGGGACCAGA TGCAGTTTTG GGAGGATGCT TTCCTAGACG CTGTTATGCT GGAGAGAGAA
    GGAATGGGTA TGGACCAAGG CCCACAGGAG ATGATAGACA GGTATTTCTC TCTTGGCGAC
    CATGACCGGA AGCGCTTAGA GGATGATGAG GATCGCCTGC TGGCCACTCT TTTGCACAAT
    ATGATTGCCT ATATGATCAT GATGAAGGTG AATAAGACAG ATCTACGCAG GAAGGTCAGG
    CGTATCCTTG GAAAGTGCCA CATAGGCTTA GTGCACAGCC AACAAATTAA TGAACTGCTG
    GACAGGGTGA CTGACATGAA TGGCCGCGAT CTCAGCATTC GTCCCAGTGG GAGCCGTCAC
    ATCAAGAAGC AAACCTTTGT AGTGCATGCT GGGACAGACA CAAGTGGGGA CCTTTTCTTT
    ATGGAGGTTT GTGACGACTG CATCGTCCTT CGCAGCAACA TCGGTGCCGT GTATGAACGT
    TGGTGGTACG AAAAACTTAT TAACATGACT TATTGTCCCA AGACTAAAGT GCTCTGTCTG
    TGGAGGAGAA ATGGCCAAGA AACTCAGCTC AACAAATTCT TCACCAAGAA GTGCCGGGAG
    CTGTACTACT GTGTGAAGGA GAGCATGGAA CGGGCGGCAG CACGACAGCA GAGCATAAAG
    CCAGGGCCGG AACTTGGCGG AGAGTTTCCT GTCCAGGACA TGAAGACAGG AGAAGGGGGT
    CTTCTTCAAG TTACCTTGGA GGGAATTAAC CTAAAATTCA TGCACAGTCA GGAGCGGAAG
    GTTTTCATAC AGCTGAATCA TATTAAAAAG TGTAACACTG TACGGGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAC TAAAGAAGTG GTGAGCCACA AATACAAGAC ACCCATGGCC
    CATGAGATCT GCTACTCCGT CCTGTGTCTT TTCTCCTACG TGGCCGCCAT CAGAGGAAAG
    GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG CCTGTGTCAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29551
    Clone ID Related Accession (Same CDS sequence) XM_012961397.2
    Accession Version XM_012961397.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816851.1
    CDS175..4836
    Translation

    Target ORF information:

    RefSeq Version XM_012961397.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961397.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT
    CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG
    GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC
    GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT
    ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA
    ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA
    GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA
    TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT
    GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT
    TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC
    TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29552
    Clone ID Related Accession (Same CDS sequence) XM_012961398.2
    Accession Version XM_012961398.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816852.1
    CDS174..4775
    Translation

    Target ORF information:

    RefSeq Version XM_012961398.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961398.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT
    CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG
    GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA
    GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT
    CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA
    CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC
    CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG
    CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG
    TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT
    CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA
    TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC
    TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC
    TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT
    CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT
    GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA
    GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT
    ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT
    GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG
    CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC
    TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC
    TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG
    CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT
    CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC
    TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG
    CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT
    GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT
    CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG
    GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA
    TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG
    ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT
    AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT
    GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT
    TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT
    GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT
    GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC
    ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT
    TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG
    AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC
    CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG
    GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA
    GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC
    TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG
    AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG
    CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC
    CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG
    ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT
    CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT
    AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG
    CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC
    AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG
    ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC
    AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT
    TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC
    AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG
    GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC
    CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA
    AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29553
    Clone ID Related Accession (Same CDS sequence) XM_018093105.1
    Accession Version XM_018093105.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product MAP kinase-activating death domain protein isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948594.1
    CDS174..4718
    Translation

    Target ORF information:

    RefSeq Version XM_018093105.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093105.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
    AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG
    GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA
    TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT
    CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT
    TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT
    GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT
    GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC
    AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA
    TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG
    GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG
    CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC
    TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC
    CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA
    CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG
    GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG
    CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT
    GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC
    CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA
    GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC
    CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA
    TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC
    CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT
    AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG
    GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG
    CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC
    AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG
    TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT
    GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT
    GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT
    GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT
    TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC
    ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT
    GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA
    CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC
    GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG
    GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG
    AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC
    CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG
    AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT
    CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT
    TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG
    TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG
    CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA
    CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA
    TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG
    GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG
    CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC
    CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG
    TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC
    AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC
    TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC
    CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG
    CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT
    GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG
    CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC
    CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC
    ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA
    GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC
    CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG
    AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA
    AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG
    GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT
    TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG
    ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG
    GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG
    AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA
    GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT
    CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT
    GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT
    CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT
    TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC
    AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA
    CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT
    GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC
    CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.