madd cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	madd
Entrez Gene ID	780174
Full Name	MAP-kinase activating death domain
General protein information	Preferred Names MAP-kinase activating death domain Names MAP kinase-activating death domain protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012961377.2	XP_012816831.1	MAP kinase-activating death domain protein isoform X7
XM_012961389.2	XP_012816843.1	MAP kinase-activating death domain protein isoform X18
XM_012961390.2	XP_012816844.1	MAP kinase-activating death domain protein isoform X19
XM_012961392.2	XP_012816846.1	MAP kinase-activating death domain protein isoform X21
XM_012961394.2	XP_012816848.1	MAP kinase-activating death domain protein isoform X23
XM_012961371.3	XP_012816825.1	MAP kinase-activating death domain protein isoform X2
XM_031900237.1	XP_031756097.1	MAP kinase-activating death domain protein isoform X14
XM_031900238.1	XP_031756098.1	MAP kinase-activating death domain protein isoform X15
XM_031900242.1	XP_031756102.1	MAP kinase-activating death domain protein isoform X22
XM_012961388.3	XP_012816842.1	MAP kinase-activating death domain protein isoform X26
XM_012961393.3	XP_012816847.1	MAP kinase-activating death domain protein isoform X31
XM_031900249.1	XP_031756109.1	MAP kinase-activating death domain protein isoform X37
XM_012961397.3	XP_012816851.1	MAP kinase-activating death domain protein isoform X39
XM_012961398.3	XP_012816852.1	MAP kinase-activating death domain protein isoform X43
XM_031900257.1	XP_031756117.1	MAP kinase-activating death domain protein isoform X47
NM_001079249.1	NP_001072717.1	MAP kinase-activating death domain protein
XM_012961370.2	XP_012816824.1	MAP kinase-activating death domain protein isoform X1
XM_012961378.2	XP_012816832.1	MAP kinase-activating death domain protein isoform X8
XM_012961384.2	XP_012816838.1	MAP kinase-activating death domain protein isoform X14
XM_012961388.2	XP_012816842.1	MAP kinase-activating death domain protein isoform X17
XM_012961398.2	XP_012816852.1	MAP kinase-activating death domain protein isoform X26
XM_012961377.3	XP_012816831.1	MAP kinase-activating death domain protein isoform X10
XM_031900236.1	XP_031756096.1	MAP kinase-activating death domain protein isoform X13
XM_012961386.3	XP_012816840.1	MAP kinase-activating death domain protein isoform X24
XM_012961387.3	XP_012816841.1	MAP kinase-activating death domain protein isoform X25
XM_031900243.1	XP_031756103.1	MAP kinase-activating death domain protein isoform X27
XM_012961390.3	XP_012816844.1	MAP kinase-activating death domain protein isoform X28
XM_012961394.3	XP_012816848.1	MAP kinase-activating death domain protein isoform X34
XM_012961368.2	XP_012816822.1	MAP kinase-activating death domain protein isoform X1
XM_012961372.2	XP_012816826.1	MAP kinase-activating death domain protein isoform X3
XM_012961373.2	XP_012816827.1	MAP kinase-activating death domain protein isoform X4
XM_012961376.2	XP_012816830.1	MAP kinase-activating death domain protein isoform X6
XM_012961387.2	XP_012816841.1	MAP kinase-activating death domain protein isoform X16
XM_012961393.2	XP_012816847.1	MAP kinase-activating death domain protein isoform X22
XM_012961368.3	XP_012816822.1	MAP kinase-activating death domain protein isoform X1
XM_031900232.1	XP_031756092.1	MAP kinase-activating death domain protein isoform X4
XM_012961373.3	XP_012816827.1	MAP kinase-activating death domain protein isoform X5
XM_012961376.3	XP_012816830.1	MAP kinase-activating death domain protein isoform X8
XM_031900240.1	XP_031756100.1	MAP kinase-activating death domain protein isoform X19
XM_012961383.3	XP_012816837.1	MAP kinase-activating death domain protein isoform X21
XM_012961391.3	XP_012816845.1	MAP kinase-activating death domain protein isoform X29
XM_031900244.1	XP_031756104.1	MAP kinase-activating death domain protein isoform X30
XM_031900247.1	XP_031756107.1	MAP kinase-activating death domain protein isoform X35
XM_031900248.1	XP_031756108.1	MAP kinase-activating death domain protein isoform X36
XM_031900252.1	XP_031756112.1	MAP kinase-activating death domain protein isoform X41
XM_031900254.1	XP_031756114.1	MAP kinase-activating death domain protein isoform X44
XM_031900256.1	XP_031756116.1	MAP kinase-activating death domain protein isoform X46
XM_012961381.2	XP_012816835.1	MAP kinase-activating death domain protein isoform X11
XM_012961383.2	XP_012816837.1	MAP kinase-activating death domain protein isoform X13
XM_012961386.2	XP_012816840.1	MAP kinase-activating death domain protein isoform X15
XM_012961395.2	XP_012816849.1	MAP kinase-activating death domain protein isoform X24
XM_018093105.1	XP_017948594.1	MAP kinase-activating death domain protein isoform X27
XM_012961370.3	XP_012816824.1	MAP kinase-activating death domain protein isoform X1
XM_012961375.3	XP_012816829.1	MAP kinase-activating death domain protein isoform X6
XM_031900233.1	XP_031756093.1	MAP kinase-activating death domain protein isoform X7
XM_012961380.3	XP_012816834.1	MAP kinase-activating death domain protein isoform X17
XM_012961381.3	XP_012816835.1	MAP kinase-activating death domain protein isoform X18
XM_012961384.3	XP_012816838.1	MAP kinase-activating death domain protein isoform X23
XM_031900246.1	XP_031756106.1	MAP kinase-activating death domain protein isoform X33
XM_031900250.1	XP_031756110.1	MAP kinase-activating death domain protein isoform X38
XM_031900251.1	XP_031756111.1	MAP kinase-activating death domain protein isoform X40
XM_031900255.1	XP_031756115.1	MAP kinase-activating death domain protein isoform X45
XM_018093105.2	XP_017948594.1	MAP kinase-activating death domain protein isoform X48
XM_012961371.2	XP_012816825.1	MAP kinase-activating death domain protein isoform X2
XM_012961375.2	XP_012816829.1	MAP kinase-activating death domain protein isoform X5
XM_012961379.2	XP_012816833.1	MAP kinase-activating death domain protein isoform X9
XM_012961380.2	XP_012816834.1	MAP kinase-activating death domain protein isoform X10
XM_012961382.2	XP_012816836.1	MAP kinase-activating death domain protein isoform X12
XM_012961391.2	XP_012816845.1	MAP kinase-activating death domain protein isoform X20
XM_012961397.2	XP_012816851.1	MAP kinase-activating death domain protein isoform X25
XM_012961372.3	XP_012816826.1	MAP kinase-activating death domain protein isoform X3
XM_031900234.1	XP_031756094.1	MAP kinase-activating death domain protein isoform X9
XM_031900235.1	XP_031756095.1	MAP kinase-activating death domain protein isoform X11
XM_012961378.3	XP_012816832.1	MAP kinase-activating death domain protein isoform X12
XM_031900239.1	XP_031756099.1	MAP kinase-activating death domain protein isoform X16
XM_031900241.1	XP_031756101.1	MAP kinase-activating death domain protein isoform X20
XM_031900245.1	XP_031756105.1	MAP kinase-activating death domain protein isoform X32
XM_031900253.1	XP_031756113.1	MAP kinase-activating death domain protein isoform X42

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MADD	NP_001180007.1
Rattus norvegicus (Norway rat)	Madd	NP_446037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000630	XP_311160.5
Caenorhabditis elegans (roundworm)	aex-3	NP_741710.1
Xenopus tropicalis (tropical clawed frog)	madd	NP_001072717.1
Mus musculus (house mouse)	Madd	NP_001171191.1
Danio rerio (zebrafish)	madd	XP_005169012.1
	MADD	XP_001107689.1
Homo sapiens (human)	MADD	NP_003673.3
Pan troglodytes (chimpanzee)	MADD	XP_003951918.1
Canis lupus familiaris (dog)	MADD	XP_005631300.1
Gallus gallus (chicken)	MADD	XP_004941623.1
Drosophila melanogaster (fruit fly)	rab3-GEF	NP_001096983.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following madd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the madd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa56194	XM_012961370.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56194	XM_012961368.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56195	XM_012961371.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56196	XM_012961372.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56197	XM_031900232.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56198	XM_012961373.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56199	XM_012961375.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56200	XM_031900233.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56201	XM_012961376.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56202	XM_031900234.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56203	XM_012961377.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56204	XM_031900235.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56205	XM_012961378.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56206	XM_031900236.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56207	XM_031900237.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56208	XM_031900238.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56209	XM_031900239.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56210	XM_012961380.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56211	XM_012961381.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56212	XM_031900240.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56213	XM_031900241.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56214	XM_012961383.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56215	XM_031900242.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56216	XM_012961384.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56217	XM_012961386.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56218	XM_012961387.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56219	XM_012961388.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56220	XM_031900243.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56221	XM_012961390.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56222	XM_012961391.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56223	XM_031900244.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56224	XM_012961393.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56225	XM_031900245.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56226	XM_031900246.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56227	XM_012961394.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56228	XM_031900247.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56229	XM_031900248.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56230	XM_031900249.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56231	XM_031900250.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56232	XM_012961397.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56233	XM_031900251.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56234	XM_031900252.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56235	XM_031900253.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56236	XM_012961398.3 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56237	XM_031900254.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56238	XM_031900255.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56239	XM_031900256.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56240	XM_031900257.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X48, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa56241	XM_018093105.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X49, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa22139	NM_001079249.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29527	XM_012961370.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29527	XM_012961368.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29528	XM_012961371.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29529	XM_012961372.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29530	XM_012961373.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29531	XM_012961375.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29532	XM_012961376.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29533	XM_012961377.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29534	XM_012961378.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29535	XM_012961379.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29536	XM_012961380.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29537	XM_012961381.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29538	XM_012961382.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29539	XM_012961383.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29540	XM_012961384.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29541	XM_012961386.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29542	XM_012961387.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29543	XM_012961388.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29544	XM_012961389.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29545	XM_012961390.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29546	XM_012961391.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29547	XM_012961392.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29548	XM_012961393.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29549	XM_012961394.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29550	XM_012961395.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29551	XM_012961397.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29552	XM_012961398.2 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29553	XM_018093105.1 Latest version!	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

madd ( XM_012961368.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816822.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

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CloneID	OXa56194
Clone ID Related Accession (Same CDS sequence)	XM_012961368.3 , XM_012961370.3
Accession Version	XM_012961368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961368.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816822.1
CDS	343..5331
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961368.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961368.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961370.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816824.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa56194
Clone ID Related Accession (Same CDS sequence)	XM_012961368.3 , XM_012961370.3
Accession Version	XM_012961370.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961370.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816824.1
CDS	570..5558
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961370.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961370.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961371.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816825.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA.

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CloneID	OXa56195
Clone ID Related Accession (Same CDS sequence)	XM_012961371.3
Accession Version	XM_012961371.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961371.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816825.1
CDS	343..5328
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961371.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961371.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961372.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816826.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

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CloneID	OXa56196
Clone ID Related Accession (Same CDS sequence)	XM_012961372.3
Accession Version	XM_012961372.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961372.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816826.1
CDS	343..5322
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961372.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961372.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900232.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756092.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

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CloneID	OXa56197
Clone ID Related Accession (Same CDS sequence)	XM_031900232.1
Accession Version	XM_031900232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGTTTTCAT ACAGCTGAAT CATATTAAAA AGTGTAACAC TGTACGGGGC 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAATACAAG 
ACACCCATGG CCCATGAGAT CTGCTACTCC GTCCTGTGTC TTTTCTCCTA CGTGGCCGCC 
ATCAGAGGAA AGGAGGCAGA GCTGAAGAGT AGACCCCCTC GGCCTGTGTC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756092.1
CDS	343..5319
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900232.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900232.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGTTTTCAT ACAGCTGAAT CATATTAAAA AGTGTAACAC TGTACGGGGC 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAATACAAG 
ACACCCATGG CCCATGAGAT CTGCTACTCC GTCCTGTGTC TTTTCTCCTA CGTGGCCGCC 
ATCAGAGGAA AGGAGGCAGA GCTGAAGAGT AGACCCCCTC GGCCTGTGTC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961373.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816827.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA.

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CloneID	OXa56198
Clone ID Related Accession (Same CDS sequence)	XM_012961373.3
Accession Version	XM_012961373.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961373.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816827.1
CDS	343..5277
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961373.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961373.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961375.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816829.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA.

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CloneID	OXa56199
Clone ID Related Accession (Same CDS sequence)	XM_012961375.3
Accession Version	XM_012961375.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961375.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816829.1
CDS	343..5274
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961375.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961375.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900233.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756093.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa56200
Clone ID Related Accession (Same CDS sequence)	XM_031900233.1
Accession Version	XM_031900233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756093.1
CDS	343..5274
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900233.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900233.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961376.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816830.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA.

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CloneID	OXa56201
Clone ID Related Accession (Same CDS sequence)	XM_012961376.3
Accession Version	XM_012961376.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961376.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816830.1
CDS	343..5271
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961376.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961376.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900234.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756094.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA.

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CloneID	OXa56202
Clone ID Related Accession (Same CDS sequence)	XM_031900234.1
Accession Version	XM_031900234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756094.1
CDS	343..5271
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900234.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900234.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961377.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816831.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA.

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CloneID	OXa56203
Clone ID Related Accession (Same CDS sequence)	XM_012961377.3
Accession Version	XM_012961377.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816831.1
CDS	343..5268
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961377.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961377.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900235.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756095.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA.

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CloneID	OXa56204
Clone ID Related Accession (Same CDS sequence)	XM_031900235.1
Accession Version	XM_031900235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756095.1
CDS	343..5268
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900235.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900235.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961378.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816832.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA.

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CloneID	OXa56205
Clone ID Related Accession (Same CDS sequence)	XM_012961378.3
Accession Version	XM_012961378.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816832.1
CDS	343..5265
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961378.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961378.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900236.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756096.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OXa56206
Clone ID Related Accession (Same CDS sequence)	XM_031900236.1
Accession Version	XM_031900236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756096.1
CDS	343..5265
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900236.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900236.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900237.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756097.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA.

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CloneID	OXa56207
Clone ID Related Accession (Same CDS sequence)	XM_031900237.1
Accession Version	XM_031900237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756097.1
CDS	343..5262
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900237.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900237.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900238.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756098.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA.

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CloneID	OXa56208
Clone ID Related Accession (Same CDS sequence)	XM_031900238.1
Accession Version	XM_031900238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGTTTTCAT ACAGCTGAAT CATATTAAAA AGTGTAACAC TGTACGGGGC 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAATACAAG 
ACACCCATGG CCCATGAGAT CTGCTACTCC GTCCTGTGTC TTTTCTCCTA CGTGGCCGCC 
ATCAGAGGAA AGGAGGCAGA GCTGAAGAGT AGACCCCCTC GGCCTGTGTC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756098.1
CDS	343..5259
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900238.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900238.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGTTTTCAT ACAGCTGAAT CATATTAAAA AGTGTAACAC TGTACGGGGC 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAATACAAG 
ACACCCATGG CCCATGAGAT CTGCTACTCC GTCCTGTGTC TTTTCTCCTA CGTGGCCGCC 
ATCAGAGGAA AGGAGGCAGA GCTGAAGAGT AGACCCCCTC GGCCTGTGTC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900239.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756099.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA.

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CloneID	OXa56209
Clone ID Related Accession (Same CDS sequence)	XM_031900239.1
Accession Version	XM_031900239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA 
ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT 
GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG 
GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT 
GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG 
GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC 
TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC 
GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT 
GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC 
CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG 
ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG 
CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG 
GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT 
CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT 
AACCTAAAAT TCATGCACAG TCAGGAGCGG AAGGTTTTCA TACAGCTGAA TCATATTAAA 
AAGTGTAACA CTGTACGGGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AACTAAAGAA 
GTGGTGAGCC ACAAATACAA GACACCCATG GCCCATGAGA TCTGCTACTC CGTCCTGTGT 
CTTTTCTCCT ACGTGGCCGC CATCAGAGGA AAGGAGGCAG AGCTGAAGAG TAGACCCCCT 
CGGCCTGTGT CAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756099.1
CDS	343..5220
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900239.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900239.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA 
ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT 
GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG 
GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT 
GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG 
GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC 
TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC 
GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT 
GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC 
CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG 
ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG 
CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG 
GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT 
CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT 
AACCTAAAAT TCATGCACAG TCAGGAGCGG AAGGTTTTCA TACAGCTGAA TCATATTAAA 
AAGTGTAACA CTGTACGGGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AACTAAAGAA 
GTGGTGAGCC ACAAATACAA GACACCCATG GCCCATGAGA TCTGCTACTC CGTCCTGTGT 
CTTTTCTCCT ACGTGGCCGC CATCAGAGGA AAGGAGGCAG AGCTGAAGAG TAGACCCCCT 
CGGCCTGTGT CAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961380.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816834.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA.

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CloneID	OXa56210
Clone ID Related Accession (Same CDS sequence)	XM_012961380.3
Accession Version	XM_012961380.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816834.1
CDS	343..5214
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961380.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961380.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961381.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816835.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA.

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CloneID	OXa56211
Clone ID Related Accession (Same CDS sequence)	XM_012961381.3
Accession Version	XM_012961381.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961381.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816835.1
CDS	343..5205
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961381.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961381.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900240.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756100.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OXa56212
Clone ID Related Accession (Same CDS sequence)	XM_031900240.1
Accession Version	XM_031900240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756100.1
CDS	343..5202
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900240.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900240.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900241.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756101.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA.

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CloneID	OXa56213
Clone ID Related Accession (Same CDS sequence)	XM_031900241.1
Accession Version	XM_031900241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA 
ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT 
GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG 
GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT 
GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG 
GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC 
TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC 
GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT 
GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC 
CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG 
ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG 
CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG 
GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT 
CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT 
AACCTAAAAT TCATGCACAG TCAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756101.1
CDS	343..5151
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900241.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900241.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA 
ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT 
GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG 
GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT 
GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG 
GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC 
TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC 
GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT 
GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC 
CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG 
ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG 
CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG 
GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT 
CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT 
AACCTAAAAT TCATGCACAG TCAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961383.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816837.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA.

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CloneID	OXa56214
Clone ID Related Accession (Same CDS sequence)	XM_012961383.3
Accession Version	XM_012961383.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961383.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816837.1
CDS	343..5148
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961383.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961383.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900242.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756102.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA.

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CloneID	OXa56215
Clone ID Related Accession (Same CDS sequence)	XM_031900242.1
Accession Version	XM_031900242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756102.1
CDS	343..5148
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900242.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900242.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961384.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816838.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OXa56216
Clone ID Related Accession (Same CDS sequence)	XM_012961384.3
Accession Version	XM_012961384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816838.1
CDS	343..5145
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961384.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961384.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961386.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816840.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OXa56217
Clone ID Related Accession (Same CDS sequence)	XM_012961386.3
Accession Version	XM_012961386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816840.1
CDS	343..5142
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961386.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961386.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961387.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816841.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA.

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CloneID	OXa56218
Clone ID Related Accession (Same CDS sequence)	XM_012961387.3
Accession Version	XM_012961387.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961387.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AGTTGTGGAA CACGCATCGG GAAGTGAAAA AGCAAAAAGC TATGGATAAA 
CAGAGTACCA AGCCAGCACC GCCTTTGGTT GATAGTGGGG AAACAGAGGA AAAGAAGTCC 
CAGATTAGCG CCGATAGTGG CCTGAGCGTC ACATCTGGTT CTCAGCGAAG CGATCTAGAC 
ACGGGCAGCA TAGGACCCTC CATCCTTATC CGAAGTAGCA GCCAGGATTC GGAAATCAGC 
ACAGTGGTGA GTAACAGCTC AGGGGAAACA CTGGGAGCCG ACAGCGACTT AAGTAGCAAC 
GCAGGGGATG CCTTGGGCGG CCCAGGAGGA AGCCACCTCT CGGGATCCAG AGGGGCTGTG 
TCCGACAGCG AGATTCAGAT GAACTCATTC ACCGGAGCCA TCTTTGGAAA GCCTCAGATG 
CTCCGCCCTG GTGAGAAAGA AAGGAATGTG AGTCCTGGGC GCCCCCAGCA AGACTTGAGC 
CAACGCATAT ACCTGTATGA GGGGCTTTTA GGAAGAGATA AGGGCTCAGT CTGGGACCAG 
CTGGAAGATG CAGCCATGGA AACCTTCTCA TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC 
CAGATGCAGT TTTGGGAGGA TGCTTTCCTA GACGCTGTTA TGCTGGAGAG AGAAGGAATG 
GGTATGGACC AAGGCCCACA GGAGATGATA GACAGGTATT TCTCTCTTGG CGACCATGAC 
CGGAAGCGCT TAGAGGATGA TGAGGATCGC CTGCTGGCCA CTCTTTTGCA CAATATGATT 
GCCTATATGA TCATGATGAA GGTGAATAAG ACAGATCTAC GCAGGAAGGT CAGGCGTATC 
CTTGGAAAGT GCCACATAGG CTTAGTGCAC AGCCAACAAA TTAATGAACT GCTGGACAGG 
GTGACTGACA TGAATGGCCG CGATCTCAGT ATTCGTCCCA GTGGGAGCCG TCACATCAAG 
AAGCAAACCT TTGTAGTGCA TGCTGGGACA GACACAAGTG GGGACCTTTT CTTTATGGAG 
GTTTGTGACG ACTGCATCGT CCTTCGCAGC AACATCGGTG CCGTGTATGA ACGTTGGTGG 
TACGAAAAAC TTATTAACAT GACTTATTGT CCCAAGACTA AAGTGCTCTG TCTGTGGAGG 
AGAAATGGCC AAGAAACTCA GCTCAACAAA TTCTTCACCA AGAAGTGCCG GGAGCTGTAC 
TACTGTGTGA AGGAGAGCAT GGAACGGGCG GCAGCACGAC AGCAGAGCAT AAAGCCAGGG 
CCGGAACTTG GCGGAGAGTT TCCTGTCCAG GACATGAAGA CAGGAGAAGG GGGTCTTCTT 
CAAGTTACCT TGGAGGGAAT TAACCTAAAA TTCATGCACA GTCAGGAGCG GAAGGTTTTC 
ATACAGCTGA ATCATATTAA AAAGTGTAAC ACTGTACGGG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAACTAAAGA AGTGGTGAGC CACAAATACA AGACACCCAT GGCCCATGAG 
ATCTGCTACT CCGTCCTGTG TCTTTTCTCC TACGTGGCCG CCATCAGAGG AAAGGAGGCA 
GAGCTGAAGA GTAGACCCCC TCGGCCTGTG TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816841.1
CDS	343..5121
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961387.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961387.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AGTTGTGGAA CACGCATCGG GAAGTGAAAA AGCAAAAAGC TATGGATAAA 
CAGAGTACCA AGCCAGCACC GCCTTTGGTT GATAGTGGGG AAACAGAGGA AAAGAAGTCC 
CAGATTAGCG CCGATAGTGG CCTGAGCGTC ACATCTGGTT CTCAGCGAAG CGATCTAGAC 
ACGGGCAGCA TAGGACCCTC CATCCTTATC CGAAGTAGCA GCCAGGATTC GGAAATCAGC 
ACAGTGGTGA GTAACAGCTC AGGGGAAACA CTGGGAGCCG ACAGCGACTT AAGTAGCAAC 
GCAGGGGATG CCTTGGGCGG CCCAGGAGGA AGCCACCTCT CGGGATCCAG AGGGGCTGTG 
TCCGACAGCG AGATTCAGAT GAACTCATTC ACCGGAGCCA TCTTTGGAAA GCCTCAGATG 
CTCCGCCCTG GTGAGAAAGA AAGGAATGTG AGTCCTGGGC GCCCCCAGCA AGACTTGAGC 
CAACGCATAT ACCTGTATGA GGGGCTTTTA GGAAGAGATA AGGGCTCAGT CTGGGACCAG 
CTGGAAGATG CAGCCATGGA AACCTTCTCA TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC 
CAGATGCAGT TTTGGGAGGA TGCTTTCCTA GACGCTGTTA TGCTGGAGAG AGAAGGAATG 
GGTATGGACC AAGGCCCACA GGAGATGATA GACAGGTATT TCTCTCTTGG CGACCATGAC 
CGGAAGCGCT TAGAGGATGA TGAGGATCGC CTGCTGGCCA CTCTTTTGCA CAATATGATT 
GCCTATATGA TCATGATGAA GGTGAATAAG ACAGATCTAC GCAGGAAGGT CAGGCGTATC 
CTTGGAAAGT GCCACATAGG CTTAGTGCAC AGCCAACAAA TTAATGAACT GCTGGACAGG 
GTGACTGACA TGAATGGCCG CGATCTCAGT ATTCGTCCCA GTGGGAGCCG TCACATCAAG 
AAGCAAACCT TTGTAGTGCA TGCTGGGACA GACACAAGTG GGGACCTTTT CTTTATGGAG 
GTTTGTGACG ACTGCATCGT CCTTCGCAGC AACATCGGTG CCGTGTATGA ACGTTGGTGG 
TACGAAAAAC TTATTAACAT GACTTATTGT CCCAAGACTA AAGTGCTCTG TCTGTGGAGG 
AGAAATGGCC AAGAAACTCA GCTCAACAAA TTCTTCACCA AGAAGTGCCG GGAGCTGTAC 
TACTGTGTGA AGGAGAGCAT GGAACGGGCG GCAGCACGAC AGCAGAGCAT AAAGCCAGGG 
CCGGAACTTG GCGGAGAGTT TCCTGTCCAG GACATGAAGA CAGGAGAAGG GGGTCTTCTT 
CAAGTTACCT TGGAGGGAAT TAACCTAAAA TTCATGCACA GTCAGGAGCG GAAGGTTTTC 
ATACAGCTGA ATCATATTAA AAAGTGTAAC ACTGTACGGG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAACTAAAGA AGTGGTGAGC CACAAATACA AGACACCCAT GGCCCATGAG 
ATCTGCTACT CCGTCCTGTG TCTTTTCTCC TACGTGGCCG CCATCAGAGG AAAGGAGGCA 
GAGCTGAAGA GTAGACCCCC TCGGCCTGTG TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961388.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816842.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA.

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CloneID	OXa56219
Clone ID Related Accession (Same CDS sequence)	XM_012961388.3
Accession Version	XM_012961388.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961388.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816842.1
CDS	343..5121
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961388.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961388.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900243.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756103.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA.

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CloneID	OXa56220
Clone ID Related Accession (Same CDS sequence)	XM_031900243.1
Accession Version	XM_031900243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGTTCCTGAA ACTAAAGAAG TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756103.1
CDS	343..5118
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900243.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900243.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGTTCCTGAA ACTAAAGAAG TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961390.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816844.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X29, mRNA.

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CloneID	OXa56221
Clone ID Related Accession (Same CDS sequence)	XM_012961390.3
Accession Version	XM_012961390.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816844.1
CDS	343..5088
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961390.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961390.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961391.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816845.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OXa56222
Clone ID Related Accession (Same CDS sequence)	XM_012961391.3
Accession Version	XM_012961391.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961391.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816845.1
CDS	343..5085
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961391.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961391.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900244.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756104.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OXa56223
Clone ID Related Accession (Same CDS sequence)	XM_031900244.1
Accession Version	XM_031900244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756104.1
CDS	343..5067
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900244.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900244.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961393.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816847.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X32, mRNA.

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CloneID	OXa56224
Clone ID Related Accession (Same CDS sequence)	XM_012961393.3
Accession Version	XM_012961393.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961393.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816847.1
CDS	343..5064
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961393.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961393.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900245.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756105.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X33, mRNA.

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CloneID	OXa56225
Clone ID Related Accession (Same CDS sequence)	XM_031900245.1
Accession Version	XM_031900245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756105.1
CDS	343..5064
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900245.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900245.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900246.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756106.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X34, mRNA.

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CloneID	OXa56226
Clone ID Related Accession (Same CDS sequence)	XM_031900246.1
Accession Version	XM_031900246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756106.1
CDS	343..5061
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900246.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900246.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961394.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816848.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OXa56227
Clone ID Related Accession (Same CDS sequence)	XM_012961394.3
Accession Version	XM_012961394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816848.1
CDS	343..5061
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961394.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961394.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900247.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756107.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X36, mRNA.

Price & Availability↑

CloneID	OXa56228
Clone ID Related Accession (Same CDS sequence)	XM_031900247.1
Accession Version	XM_031900247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGTTCCTGAA ACTAAAGAAG TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756107.1
CDS	343..5058
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900247.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900247.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACGGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGTATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGTTCCTGAA ACTAAAGAAG TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900248.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756108.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X37, mRNA.

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CloneID	OXa56229
Clone ID Related Accession (Same CDS sequence)	XM_031900248.1
Accession Version	XM_031900248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA 
ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT 
GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG 
GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT 
GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG 
GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC 
TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC 
GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT 
GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC 
CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG 
ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG 
CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG 
GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT 
CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT 
AACCTAAAAT TCATGCACAG TCAGTTCCTG AAACTAAAGA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756108.1
CDS	343..5010
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900248.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900248.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA 
ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT 
GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG 
GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT 
GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG 
GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC 
TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC 
GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT 
GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC 
CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG 
ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG 
CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG 
GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT 
CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT 
AACCTAAAAT TCATGCACAG TCAGTTCCTG AAACTAAAGA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900249.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756109.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X38, mRNA.

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CloneID	OXa56230
Clone ID Related Accession (Same CDS sequence)	XM_031900249.1
Accession Version	XM_031900249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756109.1
CDS	343..5007
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900249.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900249.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900250.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756110.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X39, mRNA.

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CloneID	OXa56231
Clone ID Related Accession (Same CDS sequence)	XM_031900250.1
Accession Version	XM_031900250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756110.1
CDS	343..5007
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900250.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900250.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961397.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816851.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X40, mRNA.

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CloneID	OXa56232
Clone ID Related Accession (Same CDS sequence)	XM_012961397.3
Accession Version	XM_012961397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816851.1
CDS	343..5004
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961397.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961397.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900251.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756111.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X41, mRNA.

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CloneID	OXa56233
Clone ID Related Accession (Same CDS sequence)	XM_031900251.1
Accession Version	XM_031900251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756111.1
CDS	343..5004
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900251.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900251.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900252.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756112.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X42, mRNA.

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CloneID	OXa56234
Clone ID Related Accession (Same CDS sequence)	XM_031900252.1
Accession Version	XM_031900252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X41
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756112.1
CDS	343..5001
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900252.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900252.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900253.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756113.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X43, mRNA.

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CloneID	OXa56235
Clone ID Related Accession (Same CDS sequence)	XM_031900253.1
Accession Version	XM_031900253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X42
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756113.1
CDS	343..4947
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900253.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900253.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961398.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816852.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X44, mRNA.

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CloneID	OXa56236
Clone ID Related Accession (Same CDS sequence)	XM_012961398.3
Accession Version	XM_012961398.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X43
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816852.1
CDS	343..4944
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961398.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961398.3

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTACTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTATGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACGG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900254.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756114.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X45, mRNA.

Price & Availability↑

CloneID	OXa56237
Clone ID Related Accession (Same CDS sequence)	XM_031900254.1
Accession Version	XM_031900254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X44
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756114.1
CDS	343..5202
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900254.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900254.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACGGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGT 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900255.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756115.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X46, mRNA.

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CloneID	OXa56238
Clone ID Related Accession (Same CDS sequence)	XM_031900255.1
Accession Version	XM_031900255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X45
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756115.1
CDS	343..5145
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900255.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900255.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGTATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900256.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756116.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X47, mRNA.

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CloneID	OXa56239
Clone ID Related Accession (Same CDS sequence)	XM_031900256.1
Accession Version	XM_031900256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X46
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756116.1
CDS	343..5208
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_031900256.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900256.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_031900257.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756117.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X48, mRNA.

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CloneID	OXa56240
Clone ID Related Accession (Same CDS sequence)	XM_031900257.1
Accession Version	XM_031900257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X47
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA 
ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT 
GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG 
GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT 
GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG 
GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC 
TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC 
GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT 
GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC 
CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG 
ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG 
CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG 
GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT 
CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT 
AACCTAAAAT TCATGCACAG TCAGTTCCTG AAACTAAAGA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756117.1
CDS	343..4950
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_031900257.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X48, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900257.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GAAGAGATAA GGGCTCAGTC TGGGACCAGC TGGAAGATGC AGCCATGGAA 
ACCTTCTCAT TGAGTAAGGA GCGTTCCACT CTGTGGGACC AGATGCAGTT TTGGGAGGAT 
GCTTTCCTAG ACGCTGTTAT GCTGGAGAGA GAAGGAATGG GTATGGACCA AGGCCCACAG 
GAGATGATAG ACAGGTATTT CTCTCTTGGC GACCATGACC GGAAGCGCTT AGAGGATGAT 
GAGGATCGCC TGCTGGCCAC TCTTTTGCAC AATATGATTG CCTATATGAT CATGATGAAG 
GTGAATAAGA CAGATCTACG CAGGAAGGTC AGGCGTATCC TTGGAAAGTG CCACATAGGC 
TTAGTGCACA GCCAACAAAT TAATGAACTG CTGGACAGGG TGACTGACAT GAATGGCCGC 
GATCTCAGTA TTCGTCCCAG TGGGAGCCGT CACATCAAGA AGCAAACCTT TGTAGTGCAT 
GCTGGGACAG ACACAAGTGG GGACCTTTTC TTTATGGAGG TTTGTGACGA CTGCATCGTC 
CTTCGCAGCA ACATCGGTGC CGTGTATGAA CGTTGGTGGT ACGAAAAACT TATTAACATG 
ACTTATTGTC CCAAGACTAA AGTGCTCTGT CTGTGGAGGA GAAATGGCCA AGAAACTCAG 
CTCAACAAAT TCTTCACCAA GAAGTGCCGG GAGCTGTACT ACTGTGTGAA GGAGAGCATG 
GAACGGGCGG CAGCACGACA GCAGAGCATA AAGCCAGGGC CGGAACTTGG CGGAGAGTTT 
CCTGTCCAGG ACATGAAGAC AGGAGAAGGG GGTCTTCTTC AAGTTACCTT GGAGGGAATT 
AACCTAAAAT TCATGCACAG TCAGTTCCTG AAACTAAAGA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_018093105.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948594.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X49, mRNA.

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CloneID	OXa56241
Clone ID Related Accession (Same CDS sequence)	XM_018093105.2
Accession Version	XM_018093105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X48
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948594.1
CDS	343..4887
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_018093105.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X49, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093105.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTTTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCTGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTACTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTATGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CGGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGTATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( NM_001079249.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072717.1 Xenopus tropicalis MAP-kinase activating death domain (madd), mRNA.

Price & Availability↑

CloneID	OXa22139
Clone ID Related Accession (Same CDS sequence)	NM_001079249.1
Accession Version	NM_001079249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125676.1. ##Evidence-Data-START## Transcript exon combination :: BC125676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028292 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG 
AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT 
CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA 
ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT 
AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG 
GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT 
CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC 
TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGTA AGGAGCGTTC CACTCTGTGG 
GACCAGATGC AGTTTTGGGA GGATGCTTTC CTAGACGCTG TTATGCTGGA GAGAGAAGGA 
ATGGGTATGG ACCAAGGCCC ACAGGAGATG ATAGACAGGT ATTTCTCTCT TGGCGACCAT 
GACCGGAAGC GCTTAGAGGA TGATGAGGAT CGCCTGCTGG CCACTCTTTT GCACAATATG 
ATTGCCTATA TGATCATGAT GAAGGTGAAT AAGACAGATC TACGCAGGAA GGTCAGGCGT 
ATCCTTGGAA AGTGCCACAT AGGCTTAGTG CACAGCCAAC AAATTAATGA ACTGCTGGAC 
AGGGTGACTG ACATGAATGG CCGCGATCTC AGTATTCGTC CCAGTGGGAG CCGTCACATC 
AAGAAGCAAA CCTTTGTAGT GCATGCTGGG ACAGACACAA GTGGGGACCT TTTCTTTATG 
GAGGTTTGTG ACGACTGCAT CGTCCTTCGC AGCAACATCG GTGCCGTGTA TGAACGTTGG 
TGGTACGAAA AACTTATTAA CATGACTTAT TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG 
AGGAGAAATG GCCAAGAAAC TCAGCTCAAC AAATTCTTCA CCAAGAAGTG CCGGGAGCTG 
TACTACTGTG TGAAGGAGAG CATGGAACGG GCGGCAGCAC GACAGCAGAG CATAAAGCCA 
GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC CAGGACATGA AGACAGGAGA AGGGGGTCTT 
CTTCAAGTTA CCTTGGAGGG AATTAACCTA AAATTCATGC ACAGTCAGGT TTTCATACAG 
CTGAATCATA TTAAAAAGTG TAACACTGTA CGGGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAACTA AAGAAGTGGT GAGCCACAAA TACAAGACAC CCATGGCCCA TGAGATCTGC 
TACTCCGTCC TGTGTCTTTT CTCCTACGTG GCCGCCATCA GAGGAAAGGA GGCAGAGCTG 
AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072717.1
CDS	95..4687
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	NM_001079249.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079249.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG 
AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT 
CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA 
ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT 
AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG 
GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT 
CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC 
TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGTA AGGAGCGTTC CACTCTGTGG 
GACCAGATGC AGTTTTGGGA GGATGCTTTC CTAGACGCTG TTATGCTGGA GAGAGAAGGA 
ATGGGTATGG ACCAAGGCCC ACAGGAGATG ATAGACAGGT ATTTCTCTCT TGGCGACCAT 
GACCGGAAGC GCTTAGAGGA TGATGAGGAT CGCCTGCTGG CCACTCTTTT GCACAATATG 
ATTGCCTATA TGATCATGAT GAAGGTGAAT AAGACAGATC TACGCAGGAA GGTCAGGCGT 
ATCCTTGGAA AGTGCCACAT AGGCTTAGTG CACAGCCAAC AAATTAATGA ACTGCTGGAC 
AGGGTGACTG ACATGAATGG CCGCGATCTC AGTATTCGTC CCAGTGGGAG CCGTCACATC 
AAGAAGCAAA CCTTTGTAGT GCATGCTGGG ACAGACACAA GTGGGGACCT TTTCTTTATG 
GAGGTTTGTG ACGACTGCAT CGTCCTTCGC AGCAACATCG GTGCCGTGTA TGAACGTTGG 
TGGTACGAAA AACTTATTAA CATGACTTAT TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG 
AGGAGAAATG GCCAAGAAAC TCAGCTCAAC AAATTCTTCA CCAAGAAGTG CCGGGAGCTG 
TACTACTGTG TGAAGGAGAG CATGGAACGG GCGGCAGCAC GACAGCAGAG CATAAAGCCA 
GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC CAGGACATGA AGACAGGAGA AGGGGGTCTT 
CTTCAAGTTA CCTTGGAGGG AATTAACCTA AAATTCATGC ACAGTCAGGT TTTCATACAG 
CTGAATCATA TTAAAAAGTG TAACACTGTA CGGGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAACTA AAGAAGTGGT GAGCCACAAA TACAAGACAC CCATGGCCCA TGAGATCTGC 
TACTCCGTCC TGTGTCTTTT CTCCTACGTG GCCGCCATCA GAGGAAAGGA GGCAGAGCTG 
AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961370.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816824.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA.

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CloneID	OXa29527
Clone ID Related Accession (Same CDS sequence)	XM_012961370.2 , XM_012961368.2
Accession Version	XM_012961370.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961370.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816824.1
CDS	816..5804
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961370.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961370.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961368.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816822.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

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CloneID	OXa29527
Clone ID Related Accession (Same CDS sequence)	XM_012961370.2 , XM_012961368.2
Accession Version	XM_012961368.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816822.1
CDS	175..5163
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961368.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961368.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961371.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816825.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA.

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CloneID	OXa29528
Clone ID Related Accession (Same CDS sequence)	XM_012961371.2
Accession Version	XM_012961371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGCATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816825.1
CDS	176..5161
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961371.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961371.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGAGTA ACAGCTCAGG GGAAACACTG 
GGAGCCGACA GCGACTTAAG TAGCAACGCA GGGGATGCCT TGGGCGGCCC AGGAGGAAGC 
CACCTCTCGG GATCCAGAGG GGCTGTGTCC GACAGCGAGA TTCAGATGAA CTCATTCACC 
GGAGCCATCT TTGGAAAGCC TCAGATGCTC CGCCCTGGTG AGAAAGAAAG GAATGTGAGT 
CCTGGGCGCC CCCAGCAAGA CTTGAGCCAA CGCATATACC TGTATGAGGG GCTTTTAGGA 
AGAGATAAGG GCTCAGTCTG GGACCAGCTG GAAGATGCAG CCATGGAAAC CTTCTCATTG 
AGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGCATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961372.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816826.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

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CloneID	OXa29529
Clone ID Related Accession (Same CDS sequence)	XM_012961372.2
Accession Version	XM_012961372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816826.1
CDS	175..5154
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961372.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961372.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961373.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816827.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

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CloneID	OXa29530
Clone ID Related Accession (Same CDS sequence)	XM_012961373.2
Accession Version	XM_012961373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816827.1
CDS	175..5109
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961373.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961373.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961375.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816829.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa29531
Clone ID Related Accession (Same CDS sequence)	XM_012961375.2
Accession Version	XM_012961375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816829.1
CDS	175..5106
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961375.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961375.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961376.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816830.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA.

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CloneID	OXa29532
Clone ID Related Accession (Same CDS sequence)	XM_012961376.2
Accession Version	XM_012961376.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816830.1
CDS	175..5103
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961376.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961376.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGAGCG GAAGGTTTTC ATACAGCTGA ATCATATTAA AAAGTGTAAC 
ACTGTACGGG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAACTAAAGA AGTGGTGAGC 
CACAAATACA AGACACCCAT GGCCCATGAG ATCTGCTACT CCGTCCTGTG TCTTTTCTCC 
TACGTGGCCG CCATCAGAGG AAAGGAGGCA GAGCTGAAGA GTAGACCCCC TCGGCCTGTG 
TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961377.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816831.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA.

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CloneID	OXa29533
Clone ID Related Accession (Same CDS sequence)	XM_012961377.2
Accession Version	XM_012961377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGCATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816831.1
CDS	175..5100
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961377.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961377.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGTAAGGAGC GTTCCACTCT GTGGGACCAG ATGCAGTTTT GGGAGGATGC TTTCCTAGAC 
GCTGTTATGC TGGAGAGAGA AGGAATGGGT ATGGACCAAG GCCCACAGGA GATGATAGAC 
AGGTATTTCT CTCTTGGCGA CCATGACCGG AAGCGCTTAG AGGATGATGA GGATCGCCTG 
CTGGCCACTC TTTTGCACAA TATGATTGCC TATATGATCA TGATGAAGGT GAATAAGACA 
GATCTACGCA GGAAGGTCAG GCGTATCCTT GGAAAGTGCC ACATAGGCTT AGTGCACAGC 
CAACAAATTA ATGAACTGCT GGACAGGGTG ACTGACATGA ATGGCCGCGA TCTCAGCATT 
CGTCCCAGTG GGAGCCGTCA CATCAAGAAG CAAACCTTTG TAGTGCATGC TGGGACAGAC 
ACAAGTGGGG ACCTTTTCTT TATGGAGGTT TGTGACGACT GCATCGTCCT TCGCAGCAAC 
ATCGGTGCCG TGTATGAACG TTGGTGGTAC GAAAAACTTA TTAACATGAC TTATTGTCCC 
AAGACTAAAG TGCTCTGTCT GTGGAGGAGA AATGGCCAAG AAACTCAGCT CAACAAATTC 
TTCACCAAGA AGTGCCGGGA GCTGTACTAC TGTGTGAAGG AGAGCATGGA ACGGGCGGCA 
GCACGACAGC AGAGCATAAA GCCAGGGCCG GAACTTGGCG GAGAGTTTCC TGTCCAGGAC 
ATGAAGACAG GAGAAGGGGG TCTTCTTCAA GTTACCTTGG AGGGAATTAA CCTAAAATTC 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961378.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816832.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA.

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CloneID	OXa29534
Clone ID Related Accession (Same CDS sequence)	XM_012961378.2
Accession Version	XM_012961378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816832.1
CDS	175..5097
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961378.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961378.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961379.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816833.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA.

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CloneID	OXa29535
Clone ID Related Accession (Same CDS sequence)	XM_012961379.2
Accession Version	XM_012961379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816833.1
CDS	175..5049
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961379.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961379.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGAA GAGATAAGGG CTCAGTCTGG GACCAGCTGG AAGATGCAGC CATGGAAACC 
TTCTCATTGA GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961380.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816834.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA.

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CloneID	OXa29536
Clone ID Related Accession (Same CDS sequence)	XM_012961380.2
Accession Version	XM_012961380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816834.1
CDS	175..5046
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961380.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961380.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGA GCGGAAGGTT TTCATACAGC TGAATCATAT TAAAAAGTGT 
AACACTGTAC GGGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAAT ACAAGACACC CATGGCCCAT GAGATCTGCT ACTCCGTCCT GTGTCTTTTC 
TCCTACGTGG CCGCCATCAG AGGAAAGGAG GCAGAGCTGA AGAGTAGACC CCCTCGGCCT 
GTGTCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961381.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816835.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa29537
Clone ID Related Accession (Same CDS sequence)	XM_012961381.2
Accession Version	XM_012961381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816835.1
CDS	175..5037
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961381.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961381.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961382.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816836.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA.

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CloneID	OXa29538
Clone ID Related Accession (Same CDS sequence)	XM_012961382.2
Accession Version	XM_012961382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816836.1
CDS	175..4989
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961382.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961382.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961383.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816837.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA.

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CloneID	OXa29539
Clone ID Related Accession (Same CDS sequence)	XM_012961383.2
Accession Version	XM_012961383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816837.1
CDS	174..4979
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961383.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961383.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961384.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816838.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA.

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CloneID	OXa29540
Clone ID Related Accession (Same CDS sequence)	XM_012961384.2
Accession Version	XM_012961384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816838.1
CDS	174..4976
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961384.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961384.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961386.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816840.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA.

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CloneID	OXa29541
Clone ID Related Accession (Same CDS sequence)	XM_012961386.2
Accession Version	XM_012961386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816840.1
CDS	174..4973
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961386.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961386.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGGTTTT CATACAGCTG AATCATATTA AAAAGTGTAA CACTGTACGG 
GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAATAC 
AAGACACCCA TGGCCCATGA GATCTGCTAC TCCGTCCTGT GTCTTTTCTC CTACGTGGCC 
GCCATCAGAG GAAAGGAGGC AGAGCTGAAG AGTAGACCCC CTCGGCCTGT GTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961387.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816841.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OXa29542
Clone ID Related Accession (Same CDS sequence)	XM_012961387.2
Accession Version	XM_012961387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AGTTGTGGAA CACGCATCGG GAAGTGAAAA AGCAAAAAGC TATGGATAAA 
CAGAGTACCA AGCCAGCACC GCCTTTGGTT GATAGTGGGG AAACAGAGGA AAAGAAGTCC 
CAGATTAGCG CCGATAGTGG CCTGAGCGTC ACATCTGGTT CTCAGCGAAG CGATCTAGAC 
ACAGGCAGCA TAGGACCCTC CATCCTTATC CGAAGTAGCA GCCAGGATTC GGAAATCAGC 
ACAGTGGTGA GTAACAGCTC AGGGGAAACA CTGGGAGCCG ACAGCGACTT AAGTAGCAAC 
GCAGGGGATG CCTTGGGCGG CCCAGGAGGA AGCCACCTCT CGGGATCCAG AGGGGCTGTG 
TCCGACAGCG AGATTCAGAT GAACTCATTC ACCGGAGCCA TCTTTGGAAA GCCTCAGATG 
CTCCGCCCTG GTGAGAAAGA AAGGAATGTG AGTCCTGGGC GCCCCCAGCA AGACTTGAGC 
CAACGCATAT ACCTGTATGA GGGGCTTTTA GGAAGAGATA AGGGCTCAGT CTGGGACCAG 
CTGGAAGATG CAGCCATGGA AACCTTCTCA TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC 
CAGATGCAGT TTTGGGAGGA TGCTTTCCTA GACGCTGTTA TGCTGGAGAG AGAAGGAATG 
GGTATGGACC AAGGCCCACA GGAGATGATA GACAGGTATT TCTCTCTTGG CGACCATGAC 
CGGAAGCGCT TAGAGGATGA TGAGGATCGC CTGCTGGCCA CTCTTTTGCA CAATATGATT 
GCCTATATGA TCATGATGAA GGTGAATAAG ACAGATCTAC GCAGGAAGGT CAGGCGTATC 
CTTGGAAAGT GCCACATAGG CTTAGTGCAC AGCCAACAAA TTAATGAACT GCTGGACAGG 
GTGACTGACA TGAATGGCCG CGATCTCAGC ATTCGTCCCA GTGGGAGCCG TCACATCAAG 
AAGCAAACCT TTGTAGTGCA TGCTGGGACA GACACAAGTG GGGACCTTTT CTTTATGGAG 
GTTTGTGACG ACTGCATCGT CCTTCGCAGC AACATCGGTG CCGTGTATGA ACGTTGGTGG 
TACGAAAAAC TTATTAACAT GACTTATTGT CCCAAGACTA AAGTGCTCTG TCTGTGGAGG 
AGAAATGGCC AAGAAACTCA GCTCAACAAA TTCTTCACCA AGAAGTGCCG GGAGCTGTAC 
TACTGTGTGA AGGAGAGCAT GGAACGGGCG GCAGCACGAC AGCAGAGCAT AAAGCCAGGG 
CCGGAACTTG GCGGAGAGTT TCCTGTCCAG GACATGAAGA CAGGAGAAGG GGGTCTTCTT 
CAAGTTACCT TGGAGGGAAT TAACCTAAAA TTCATGCACA GTCAGGAGCG GAAGGTTTTC 
ATACAGCTGA ATCATATTAA AAAGTGTAAC ACTGTACGGG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAACTAAAGA AGTGGTGAGC CACAAATACA AGACACCCAT GGCCCATGAG 
ATCTGCTACT CCGTCCTGTG TCTTTTCTCC TACGTGGCCG CCATCAGAGG AAAGGAGGCA 
GAGCTGAAGA GTAGACCCCC TCGGCCTGTG TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816841.1
CDS	175..4953
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961387.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961387.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AGTTGTGGAA CACGCATCGG GAAGTGAAAA AGCAAAAAGC TATGGATAAA 
CAGAGTACCA AGCCAGCACC GCCTTTGGTT GATAGTGGGG AAACAGAGGA AAAGAAGTCC 
CAGATTAGCG CCGATAGTGG CCTGAGCGTC ACATCTGGTT CTCAGCGAAG CGATCTAGAC 
ACAGGCAGCA TAGGACCCTC CATCCTTATC CGAAGTAGCA GCCAGGATTC GGAAATCAGC 
ACAGTGGTGA GTAACAGCTC AGGGGAAACA CTGGGAGCCG ACAGCGACTT AAGTAGCAAC 
GCAGGGGATG CCTTGGGCGG CCCAGGAGGA AGCCACCTCT CGGGATCCAG AGGGGCTGTG 
TCCGACAGCG AGATTCAGAT GAACTCATTC ACCGGAGCCA TCTTTGGAAA GCCTCAGATG 
CTCCGCCCTG GTGAGAAAGA AAGGAATGTG AGTCCTGGGC GCCCCCAGCA AGACTTGAGC 
CAACGCATAT ACCTGTATGA GGGGCTTTTA GGAAGAGATA AGGGCTCAGT CTGGGACCAG 
CTGGAAGATG CAGCCATGGA AACCTTCTCA TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC 
CAGATGCAGT TTTGGGAGGA TGCTTTCCTA GACGCTGTTA TGCTGGAGAG AGAAGGAATG 
GGTATGGACC AAGGCCCACA GGAGATGATA GACAGGTATT TCTCTCTTGG CGACCATGAC 
CGGAAGCGCT TAGAGGATGA TGAGGATCGC CTGCTGGCCA CTCTTTTGCA CAATATGATT 
GCCTATATGA TCATGATGAA GGTGAATAAG ACAGATCTAC GCAGGAAGGT CAGGCGTATC 
CTTGGAAAGT GCCACATAGG CTTAGTGCAC AGCCAACAAA TTAATGAACT GCTGGACAGG 
GTGACTGACA TGAATGGCCG CGATCTCAGC ATTCGTCCCA GTGGGAGCCG TCACATCAAG 
AAGCAAACCT TTGTAGTGCA TGCTGGGACA GACACAAGTG GGGACCTTTT CTTTATGGAG 
GTTTGTGACG ACTGCATCGT CCTTCGCAGC AACATCGGTG CCGTGTATGA ACGTTGGTGG 
TACGAAAAAC TTATTAACAT GACTTATTGT CCCAAGACTA AAGTGCTCTG TCTGTGGAGG 
AGAAATGGCC AAGAAACTCA GCTCAACAAA TTCTTCACCA AGAAGTGCCG GGAGCTGTAC 
TACTGTGTGA AGGAGAGCAT GGAACGGGCG GCAGCACGAC AGCAGAGCAT AAAGCCAGGG 
CCGGAACTTG GCGGAGAGTT TCCTGTCCAG GACATGAAGA CAGGAGAAGG GGGTCTTCTT 
CAAGTTACCT TGGAGGGAAT TAACCTAAAA TTCATGCACA GTCAGGAGCG GAAGGTTTTC 
ATACAGCTGA ATCATATTAA AAAGTGTAAC ACTGTACGGG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAACTAAAGA AGTGGTGAGC CACAAATACA AGACACCCAT GGCCCATGAG 
ATCTGCTACT CCGTCCTGTG TCTTTTCTCC TACGTGGCCG CCATCAGAGG AAAGGAGGCA 
GAGCTGAAGA GTAGACCCCC TCGGCCTGTG TCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961388.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816842.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OXa29543
Clone ID Related Accession (Same CDS sequence)	XM_012961388.2
Accession Version	XM_012961388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816842.1
CDS	176..4954
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961388.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961388.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG AGTTGTGGAA CACGCATCGG 
GAAGTGAAAA AGCAAAAAGC TATGGATAAA CAGAGTACCA AGCCAGCACC GCCTTTGGTT 
GATAGTGGGG AAACAGAGGA AAAGAAGTCC CAGATTAGCG CCGATAGTGG CCTGAGCGTC 
ACATCTGGTT CTCAGCGAAG CGATCTAGAC ACAGGCAGCA TAGGACCCTC CATCCTTATC 
CGAAGTAGCA GCCAGGATTC GGAAATCAGC ACAGTGGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961389.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816843.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA.

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CloneID	OXa29544
Clone ID Related Accession (Same CDS sequence)	XM_012961389.2
Accession Version	XM_012961389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816843.1
CDS	174..4928
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961389.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961389.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGAGCGGAAG GTTTTCATAC AGCTGAATCA TATTAAAAAG 
TGTAACACTG TACGGGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AATACAAGAC ACCCATGGCC CATGAGATCT GCTACTCCGT CCTGTGTCTT 
TTCTCCTACG TGGCCGCCAT CAGAGGAAAG GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG 
CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961390.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816844.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA.

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CloneID	OXa29545
Clone ID Related Accession (Same CDS sequence)	XM_012961390.2
Accession Version	XM_012961390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816844.1
CDS	174..4919
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961390.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961390.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GGTTTTCATA CAGCTGAATC ATATTAAAAA GTGTAACACT 
GTACGGGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAATACAAGA CACCCATGGC CCATGAGATC TGCTACTCCG TCCTGTGTCT TTTCTCCTAC 
GTGGCCGCCA TCAGAGGAAA GGAGGCAGAG CTGAAGAGTA GACCCCCTCG GCCTGTGTCA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961391.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816845.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA.

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CloneID	OXa29546
Clone ID Related Accession (Same CDS sequence)	XM_012961391.2
Accession Version	XM_012961391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816845.1
CDS	174..4916
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961391.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961391.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGGT TTTCATACAG CTGAATCATA TTAAAAAGTG TAACACTGTA 
CGGGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAA 
TACAAGACAC CCATGGCCCA TGAGATCTGC TACTCCGTCC TGTGTCTTTT CTCCTACGTG 
GCCGCCATCA GAGGAAAGGA GGCAGAGCTG AAGAGTAGAC CCCCTCGGCC TGTGTCAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961392.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816846.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OXa29547
Clone ID Related Accession (Same CDS sequence)	XM_012961392.2
Accession Version	XM_012961392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG 
AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT 
CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA 
ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT 
AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG 
GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT 
CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC 
TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGAA GAGATAAGGG CTCAGTCTGG 
GACCAGCTGG AAGATGCAGC CATGGAAACC TTCTCATTGA GTAAGGAGCG TTCCACTCTG 
TGGGACCAGA TGCAGTTTTG GGAGGATGCT TTCCTAGACG CTGTTATGCT GGAGAGAGAA 
GGAATGGGTA TGGACCAAGG CCCACAGGAG ATGATAGACA GGTATTTCTC TCTTGGCGAC 
CATGACCGGA AGCGCTTAGA GGATGATGAG GATCGCCTGC TGGCCACTCT TTTGCACAAT 
ATGATTGCCT ATATGATCAT GATGAAGGTG AATAAGACAG ATCTACGCAG GAAGGTCAGG 
CGTATCCTTG GAAAGTGCCA CATAGGCTTA GTGCACAGCC AACAAATTAA TGAACTGCTG 
GACAGGGTGA CTGACATGAA TGGCCGCGAT CTCAGCATTC GTCCCAGTGG GAGCCGTCAC 
ATCAAGAAGC AAACCTTTGT AGTGCATGCT GGGACAGACA CAAGTGGGGA CCTTTTCTTT 
ATGGAGGTTT GTGACGACTG CATCGTCCTT CGCAGCAACA TCGGTGCCGT GTATGAACGT 
TGGTGGTACG AAAAACTTAT TAACATGACT TATTGTCCCA AGACTAAAGT GCTCTGTCTG 
TGGAGGAGAA ATGGCCAAGA AACTCAGCTC AACAAATTCT TCACCAAGAA GTGCCGGGAG 
CTGTACTACT GTGTGAAGGA GAGCATGGAA CGGGCGGCAG CACGACAGCA GAGCATAAAG 
CCAGGGCCGG AACTTGGCGG AGAGTTTCCT GTCCAGGACA TGAAGACAGG AGAAGGGGGT 
CTTCTTCAAG TTACCTTGGA GGGAATTAAC CTAAAATTCA TGCACAGTCA GGAGCGGAAG 
GTTTTCATAC AGCTGAATCA TATTAAAAAG TGTAACACTG TACGGGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAC TAAAGAAGTG GTGAGCCACA AATACAAGAC ACCCATGGCC 
CATGAGATCT GCTACTCCGT CCTGTGTCTT TTCTCCTACG TGGCCGCCAT CAGAGGAAAG 
GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816846.1
CDS	175..4899
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961392.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961392.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGTATT TGGGCTTAAT 
ACTATAATGG AGATTATTGC AGACTCCGGT CCCGCCCCTG CAGAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG 
AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT 
CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA 
ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT 
AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG 
GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT 
CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC 
TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGAA GAGATAAGGG CTCAGTCTGG 
GACCAGCTGG AAGATGCAGC CATGGAAACC TTCTCATTGA GTAAGGAGCG TTCCACTCTG 
TGGGACCAGA TGCAGTTTTG GGAGGATGCT TTCCTAGACG CTGTTATGCT GGAGAGAGAA 
GGAATGGGTA TGGACCAAGG CCCACAGGAG ATGATAGACA GGTATTTCTC TCTTGGCGAC 
CATGACCGGA AGCGCTTAGA GGATGATGAG GATCGCCTGC TGGCCACTCT TTTGCACAAT 
ATGATTGCCT ATATGATCAT GATGAAGGTG AATAAGACAG ATCTACGCAG GAAGGTCAGG 
CGTATCCTTG GAAAGTGCCA CATAGGCTTA GTGCACAGCC AACAAATTAA TGAACTGCTG 
GACAGGGTGA CTGACATGAA TGGCCGCGAT CTCAGCATTC GTCCCAGTGG GAGCCGTCAC 
ATCAAGAAGC AAACCTTTGT AGTGCATGCT GGGACAGACA CAAGTGGGGA CCTTTTCTTT 
ATGGAGGTTT GTGACGACTG CATCGTCCTT CGCAGCAACA TCGGTGCCGT GTATGAACGT 
TGGTGGTACG AAAAACTTAT TAACATGACT TATTGTCCCA AGACTAAAGT GCTCTGTCTG 
TGGAGGAGAA ATGGCCAAGA AACTCAGCTC AACAAATTCT TCACCAAGAA GTGCCGGGAG 
CTGTACTACT GTGTGAAGGA GAGCATGGAA CGGGCGGCAG CACGACAGCA GAGCATAAAG 
CCAGGGCCGG AACTTGGCGG AGAGTTTCCT GTCCAGGACA TGAAGACAGG AGAAGGGGGT 
CTTCTTCAAG TTACCTTGGA GGGAATTAAC CTAAAATTCA TGCACAGTCA GGAGCGGAAG 
GTTTTCATAC AGCTGAATCA TATTAAAAAG TGTAACACTG TACGGGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAC TAAAGAAGTG GTGAGCCACA AATACAAGAC ACCCATGGCC 
CATGAGATCT GCTACTCCGT CCTGTGTCTT TTCTCCTACG TGGCCGCCAT CAGAGGAAAG 
GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961393.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816847.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OXa29548
Clone ID Related Accession (Same CDS sequence)	XM_012961393.2
Accession Version	XM_012961393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816847.1
CDS	175..4896
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961393.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961393.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961394.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816848.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA.

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CloneID	OXa29549
Clone ID Related Accession (Same CDS sequence)	XM_012961394.2
Accession Version	XM_012961394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816848.1
CDS	176..4894
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKVFGLNTIMEIIADSGPAPAEGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961394.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961394.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGT ATTTGGGCTT 
AATACTATAA TGGAGATTAT TGCAGACTCC GGTCCCGCCC CTGCAGAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGA GTAACAGCTC AGGGGAAACA 
CTGGGAGCCG ACAGCGACTT AAGTAGCAAC GCAGGGGATG CCTTGGGCGG CCCAGGAGGA 
AGCCACCTCT CGGGATCCAG AGGGGCTGTG TCCGACAGCG AGATTCAGAT GAACTCATTC 
ACCGGAGCCA TCTTTGGAAA GCCTCAGATG CTCCGCCCTG GTGAGAAAGA AAGGAATGTG 
AGTCCTGGGC GCCCCCAGCA AGACTTGAGC CAACGCATAT ACCTGTATGA GGGGCTTTTA 
GGAAGAGATA AGGGCTCAGT CTGGGACCAG CTGGAAGATG CAGCCATGGA AACCTTCTCA 
TTGAGTAAGG AGCGTTCCAC TCTGTGGGAC CAGATGCAGT TTTGGGAGGA TGCTTTCCTA 
GACGCTGTTA TGCTGGAGAG AGAAGGAATG GGTATGGACC AAGGCCCACA GGAGATGATA 
GACAGGTATT TCTCTCTTGG CGACCATGAC CGGAAGCGCT TAGAGGATGA TGAGGATCGC 
CTGCTGGCCA CTCTTTTGCA CAATATGATT GCCTATATGA TCATGATGAA GGTGAATAAG 
ACAGATCTAC GCAGGAAGGT CAGGCGTATC CTTGGAAAGT GCCACATAGG CTTAGTGCAC 
AGCCAACAAA TTAATGAACT GCTGGACAGG GTGACTGACA TGAATGGCCG CGATCTCAGC 
ATTCGTCCCA GTGGGAGCCG TCACATCAAG AAGCAAACCT TTGTAGTGCA TGCTGGGACA 
GACACAAGTG GGGACCTTTT CTTTATGGAG GTTTGTGACG ACTGCATCGT CCTTCGCAGC 
AACATCGGTG CCGTGTATGA ACGTTGGTGG TACGAAAAAC TTATTAACAT GACTTATTGT 
CCCAAGACTA AAGTGCTCTG TCTGTGGAGG AGAAATGGCC AAGAAACTCA GCTCAACAAA 
TTCTTCACCA AGAAGTGCCG GGAGCTGTAC TACTGTGTGA AGGAGAGCAT GGAACGGGCG 
GCAGCACGAC AGCAGAGCAT AAAGCCAGGG CCGGAACTTG GCGGAGAGTT TCCTGTCCAG 
GACATGAAGA CAGGAGAAGG GGGTCTTCTT CAAGTTACCT TGGAGGGAAT TAACCTAAAA 
TTCATGCACA GTCAGTTCCT GAAACTAAAG AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961395.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816849.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA.

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CloneID	OXa29550
Clone ID Related Accession (Same CDS sequence)	XM_012961395.2
Accession Version	XM_012961395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG 
AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT 
CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA 
ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT 
AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG 
GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT 
CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC 
TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGAA GAGATAAGGG CTCAGTCTGG 
GACCAGCTGG AAGATGCAGC CATGGAAACC TTCTCATTGA GTAAGGAGCG TTCCACTCTG 
TGGGACCAGA TGCAGTTTTG GGAGGATGCT TTCCTAGACG CTGTTATGCT GGAGAGAGAA 
GGAATGGGTA TGGACCAAGG CCCACAGGAG ATGATAGACA GGTATTTCTC TCTTGGCGAC 
CATGACCGGA AGCGCTTAGA GGATGATGAG GATCGCCTGC TGGCCACTCT TTTGCACAAT 
ATGATTGCCT ATATGATCAT GATGAAGGTG AATAAGACAG ATCTACGCAG GAAGGTCAGG 
CGTATCCTTG GAAAGTGCCA CATAGGCTTA GTGCACAGCC AACAAATTAA TGAACTGCTG 
GACAGGGTGA CTGACATGAA TGGCCGCGAT CTCAGCATTC GTCCCAGTGG GAGCCGTCAC 
ATCAAGAAGC AAACCTTTGT AGTGCATGCT GGGACAGACA CAAGTGGGGA CCTTTTCTTT 
ATGGAGGTTT GTGACGACTG CATCGTCCTT CGCAGCAACA TCGGTGCCGT GTATGAACGT 
TGGTGGTACG AAAAACTTAT TAACATGACT TATTGTCCCA AGACTAAAGT GCTCTGTCTG 
TGGAGGAGAA ATGGCCAAGA AACTCAGCTC AACAAATTCT TCACCAAGAA GTGCCGGGAG 
CTGTACTACT GTGTGAAGGA GAGCATGGAA CGGGCGGCAG CACGACAGCA GAGCATAAAG 
CCAGGGCCGG AACTTGGCGG AGAGTTTCCT GTCCAGGACA TGAAGACAGG AGAAGGGGGT 
CTTCTTCAAG TTACCTTGGA GGGAATTAAC CTAAAATTCA TGCACAGTCA GGAGCGGAAG 
GTTTTCATAC AGCTGAATCA TATTAAAAAG TGTAACACTG TACGGGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAC TAAAGAAGTG GTGAGCCACA AATACAAGAC ACCCATGGCC 
CATGAGATCT GCTACTCCGT CCTGTGTCTT TTCTCCTACG TGGCCGCCAT CAGAGGAAAG 
GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816849.1
CDS	174..4838
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAELKSRPPRPVSS

Target ORF information:

RefSeq Version	XM_012961395.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961395.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG GTACCAAGCC AGCACCGCCT TTGGTTGATA GTGGGGAAAC AGAGGAAAAG 
AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG AGCGTCACAT CTGGTTCTCA GCGAAGCGAT 
CTAGACACAG GCAGCATAGG ACCCTCCATC CTTATCCGAA GTAGCAGCCA GGATTCGGAA 
ATCAGCACAG TGGTGAGTAA CAGCTCAGGG GAAACACTGG GAGCCGACAG CGACTTAAGT 
AGCAACGCAG GGGATGCCTT GGGCGGCCCA GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG 
GCTGTGTCCG ACAGCGAGAT TCAGATGAAC TCATTCACCG GAGCCATCTT TGGAAAGCCT 
CAGATGCTCC GCCCTGGTGA GAAAGAAAGG AATGTGAGTC CTGGGCGCCC CCAGCAAGAC 
TTGAGCCAAC GCATATACCT GTATGAGGGG CTTTTAGGAA GAGATAAGGG CTCAGTCTGG 
GACCAGCTGG AAGATGCAGC CATGGAAACC TTCTCATTGA GTAAGGAGCG TTCCACTCTG 
TGGGACCAGA TGCAGTTTTG GGAGGATGCT TTCCTAGACG CTGTTATGCT GGAGAGAGAA 
GGAATGGGTA TGGACCAAGG CCCACAGGAG ATGATAGACA GGTATTTCTC TCTTGGCGAC 
CATGACCGGA AGCGCTTAGA GGATGATGAG GATCGCCTGC TGGCCACTCT TTTGCACAAT 
ATGATTGCCT ATATGATCAT GATGAAGGTG AATAAGACAG ATCTACGCAG GAAGGTCAGG 
CGTATCCTTG GAAAGTGCCA CATAGGCTTA GTGCACAGCC AACAAATTAA TGAACTGCTG 
GACAGGGTGA CTGACATGAA TGGCCGCGAT CTCAGCATTC GTCCCAGTGG GAGCCGTCAC 
ATCAAGAAGC AAACCTTTGT AGTGCATGCT GGGACAGACA CAAGTGGGGA CCTTTTCTTT 
ATGGAGGTTT GTGACGACTG CATCGTCCTT CGCAGCAACA TCGGTGCCGT GTATGAACGT 
TGGTGGTACG AAAAACTTAT TAACATGACT TATTGTCCCA AGACTAAAGT GCTCTGTCTG 
TGGAGGAGAA ATGGCCAAGA AACTCAGCTC AACAAATTCT TCACCAAGAA GTGCCGGGAG 
CTGTACTACT GTGTGAAGGA GAGCATGGAA CGGGCGGCAG CACGACAGCA GAGCATAAAG 
CCAGGGCCGG AACTTGGCGG AGAGTTTCCT GTCCAGGACA TGAAGACAGG AGAAGGGGGT 
CTTCTTCAAG TTACCTTGGA GGGAATTAAC CTAAAATTCA TGCACAGTCA GGAGCGGAAG 
GTTTTCATAC AGCTGAATCA TATTAAAAAG TGTAACACTG TACGGGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAC TAAAGAAGTG GTGAGCCACA AATACAAGAC ACCCATGGCC 
CATGAGATCT GCTACTCCGT CCTGTGTCTT TTCTCCTACG TGGCCGCCAT CAGAGGAAAG 
GAGGCAGAGC TGAAGAGTAG ACCCCCTCGG CCTGTGTCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961397.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816851.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA.

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CloneID	OXa29551
Clone ID Related Accession (Same CDS sequence)	XM_012961397.2
Accession Version	XM_012961397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816851.1
CDS	175..4836
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961397.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961397.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGAGTTGTG GAACACGCAT 
CGGGAAGTGA AAAAGCAAAA AGCTATGGAT AAACAGAGTA CCAAGCCAGC ACCGCCTTTG 
GTTGATAGTG GGGAAACAGA GGAAAAGAAG TCCCAGATTA GCGCCGATAG TGGCCTGAGC 
GTCACATCTG GTTCTCAGCG AAGCGATCTA GACACAGGCA GCATAGGACC CTCCATCCTT 
ATCCGAAGTA GCAGCCAGGA TTCGGAAATC AGCACAGTGG TGAGTAACAG CTCAGGGGAA 
ACACTGGGAG CCGACAGCGA CTTAAGTAGC AACGCAGGGG ATGCCTTGGG CGGCCCAGGA 
GGAAGCCACC TCTCGGGATC CAGAGGGGCT GTGTCCGACA GCGAGATTCA GATGAACTCA 
TTCACCGGAG CCATCTTTGG AAAGCCTCAG ATGCTCCGCC CTGGTGAGAA AGAAAGGAAT 
GTGAGTCCTG GGCGCCCCCA GCAAGACTTG AGCCAACGCA TATACCTGTA TGAGGGGCTT 
TTAGGAAGAG ATAAGGGCTC AGTCTGGGAC CAGCTGGAAG ATGCAGCCAT GGAAACCTTC 
TCATTGAGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_012961398.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816852.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA.

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CloneID	OXa29552
Clone ID Related Accession (Same CDS sequence)	XM_012961398.2
Accession Version	XM_012961398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816852.1
CDS	174..4775
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_012961398.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961398.2

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGTGT 
CTACACAGCA TGTCCGTGAT CACGCAGCAG CGTCTTCTCG CCTCAGATAA CAAGGCATTG 
GCTAGCATGA GTCTGAACAC TCAGCCTATC CTCAATTTGG ACCGATTTCA CGAAGGCCCA 
GACGCTGCAC TGTTGAAAGC CCCCAGTGAT GCACAGGGAA CTCCCTCCAC AGAATTTAAT 
CCTCTTATAT ATGGAAATGA TGTTGACTCT GTGGATGTGG CAACAAGGGT TGCCATGGTA 
CGTTTCTTTA ACTCCCCGAA TATGTTGCAC AGTTTCCAAA TGCACACACG CACCCTGCGC 
CTTTTCCCTC GTCCAGTCGT GGCTTTCCAA TCCTCTTCCT TCCTGGCAGC ACGGCCCAAG 
CCCTCCCTTT TTACTGAGAA GCTTTCCAAA ACGCAGGCAG TGGAATTCTT TGGGGAGTGG 
TCCCTGAACC CCACTAACTT TGCATTCCAG AGAATCCACA ACAACATGTT TGACCCTGCT 
CTGATTGGGG ATAAGCCCAA GTGGTATGCA CACCAGCTGC AGCCCATTCA CTACCGTGTA 
TATGATGCTA ACTCACAGCT TGCGGAAGCA TTTTGTGTGC CAGCAGAGAG GGACAGTGAC 
TCTGATCCTA CTGATGACAG TGGCAGCGAT AGTGTGGAAT ATGATGACTC CAGCTCTTCC 
TACTCTTCTC TTGGAGACTT TGTCAGCGAA ATGATGAAAT GTGATATTAA TGGGGACACT 
CCAAATGTAG ATCCGCTGAC GCATGCAGCG CTTGGAGATG CCAGTGAGGT TGAATTTGAT 
GACTTCCAAG AATACTCAGG AGAAATAGAG GAGAACAATG GCGATAGCGA GAACTCACAA 
GATAACAACC AGCCTCGCTC TGGATCTAGC ACCACAGCCA GCAGCAGCCC CAGTACCGTT 
ATACACGGGG CTAACACGGA ATCAGCCGAT TCTGCAGAGC CCGAAGAGAA AGTTATGGGT 
GTGTTGCAAA ATCACTTGCC AGTTTTGCCA CCCACATTCA CCCGGTTAGC CATGGAGAGG 
CGGGAGAGTG ATGTGTCACT GGGTGGCGGA GAGGCACGAA GACGAGAGTA TGACAACCCC 
TACTTTGAGC CACAGTATGG ATTTCCTCCA GAGGAGGAGA ATGATGAACG GGAGGAGAGC 
TATACACCGA TGTTTAACCA GAGTGTGAAT GGTACCCGAT CCCCGAAGCC TCTGCTTCGG 
CCCAATAGCT TGAAGCTTGC AAGTGATTCT GATGCCGAGA CAGATTCTCG GGGTAGTTCT 
CCCACGTCAA CTGTGTCCAA CAACAGCAGC AATAACGAAG GCTTTGGAGG CATCATGTCC 
TTCGCCAGCA ACCTGTATAA AACTCACAGC AGTAGCTTTA GCTTATCGAG CCTGACGCTG 
CCGAGCAAAA GCCGAGACAA GGCCACTCCC TTCCCCAGCC TGAAAGGCAG TAACAGACGT 
GCTCTTGTTG ACCAGAAGTC TTCAGGTATA AAGCACAGCC CTACAGTCAA GCGAGAATCT 
CCCTCTCCTC AGGGAAAATC AAACAACTCC AGTGAGAACC AGCAGTTCCT AAAGGAGGTG 
GCGCTGGGTG TTCTGGAGGG CCAAGGGGTA GGATGGCTGA ACATGAAAAA GGTGCGGCGA 
TTGCTGGAGA GCGAGCAGCT GCGCGTATTT GTGCTAAGCA AATTGAACAG GTCCATCCAG 
ACAGAAGAAG ATGCTCGCAA TGATGTCATC CAGGAAGTGG AAATAACCCG AAAAGTGTAT 
AGGGGCATGC TGGACCTGCT GAAGTGCACC GTATCGAGCC TCGAGCAGAC TTACGCCAAT 
GCTGGCCTGG GAGGAATGGC CAGTGTCTTC AGCTTACTCG AGATTGCTCA TACTCACTAT 
TACAACAAAG AACCGGAAAA AAGGAAGCAC AGTCCGTCTG CCGATGGAGC TGTCACCCCT 
GTGTCTAGGG ATCCTGTCTC AGCTCCGAGG GTAGAGTCTC GACCCGCCCC TCAGCTGCCT 
GCCAAGCAGC TCTTGCCCCG CCCATCAGGC CCCGCTGGCA GAGGGGCACG CGAGTTTGAC 
ACCCGGAGTC TTAAAGAAGA GAATTTTGTG GCATCCATTG GTACCAAGCC AGCACCGCCT 
TTGGTTGATA GTGGGGAAAC AGAGGAAAAG AAGTCCCAGA TTAGCGCCGA TAGTGGCCTG 
AGCGTCACAT CTGGTTCTCA GCGAAGCGAT CTAGACACAG GCAGCATAGG ACCCTCCATC 
CTTATCCGAA GTAGCAGCCA GGATTCGGAA ATCAGCACAG TGGTGAGTAA CAGCTCAGGG 
GAAACACTGG GAGCCGACAG CGACTTAAGT AGCAACGCAG GGGATGCCTT GGGCGGCCCA 
GGAGGAAGCC ACCTCTCGGG ATCCAGAGGG GCTGTGTCCG ACAGCGAGAT TCAGATGAAC 
TCATTCACCG GAGCCATCTT TGGAAAGCCT CAGATGCTCC GCCCTGGTGA GAAAGAAAGG 
AATGTGAGTC CTGGGCGCCC CCAGCAAGAC TTGAGCCAAC GCATATACCT GTATGAGGGG 
CTTTTAGGTA AGGAGCGTTC CACTCTGTGG GACCAGATGC AGTTTTGGGA GGATGCTTTC 
CTAGACGCTG TTATGCTGGA GAGAGAAGGA ATGGGTATGG ACCAAGGCCC ACAGGAGATG 
ATAGACAGGT ATTTCTCTCT TGGCGACCAT GACCGGAAGC GCTTAGAGGA TGATGAGGAT 
CGCCTGCTGG CCACTCTTTT GCACAATATG ATTGCCTATA TGATCATGAT GAAGGTGAAT 
AAGACAGATC TACGCAGGAA GGTCAGGCGT ATCCTTGGAA AGTGCCACAT AGGCTTAGTG 
CACAGCCAAC AAATTAATGA ACTGCTGGAC AGGGTGACTG ACATGAATGG CCGCGATCTC 
AGCATTCGTC CCAGTGGGAG CCGTCACATC AAGAAGCAAA CCTTTGTAGT GCATGCTGGG 
ACAGACACAA GTGGGGACCT TTTCTTTATG GAGGTTTGTG ACGACTGCAT CGTCCTTCGC 
AGCAACATCG GTGCCGTGTA TGAACGTTGG TGGTACGAAA AACTTATTAA CATGACTTAT 
TGTCCCAAGA CTAAAGTGCT CTGTCTGTGG AGGAGAAATG GCCAAGAAAC TCAGCTCAAC 
AAATTCTTCA CCAAGAAGTG CCGGGAGCTG TACTACTGTG TGAAGGAGAG CATGGAACGG 
GCGGCAGCAC GACAGCAGAG CATAAAGCCA GGGCCGGAAC TTGGCGGAGA GTTTCCTGTC 
CAGGACATGA AGACAGGAGA AGGGGGTCTT CTTCAAGTTA CCTTGGAGGG AATTAACCTA 
AAATTCATGC ACAGTCAGTT CCTGAAACTA AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

madd ( XM_018093105.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948594.1 Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OXa29553
Clone ID Related Accession (Same CDS sequence)	XM_018093105.1
Accession Version	XM_018093105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MAP kinase-activating death domain protein isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948594.1
CDS	174..4718
Translation	MGQRKKACPRLLDYLVIIGARQPSSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGSDGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNADLLPVLPEPEASELKKHLKQALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGEIEENNGDSENSQDNNQPRSGSSTTASSSPSTVIHGANTESADSAEPEEKVMGVLQNHLPVLPPTFTRLAMERRESDVSLGGGEARRREYDNPYFEPQYGFPPEEENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGGIMSFASNLYKTHSSSFSLSSLTLPSKSRDKATPFPSLKGSNRRALVDQKSSGIKHSPTVKRESPSPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRSLKEENFVASIGTKPAPPLVDSGETEEKKSQISADSGLSVTSGSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPGGSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_018093105.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MAP-kinase activating death domain (madd), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093105.1

ORF Insert Sequence:

ATGGGGCAAA GGAAGAAAGC GTGTCCCCGG TTGCTGGATT ACTTGGTGAT CATTGGTGCC 
AGGCAGCCGA GCAGTGATAG CATTTCTCAG ACCCCTGAGC TTCTGCGACG TTATCCTTTG 
GAGGATCATC CTGAGTTTCC GCTTCCCCCT GATGTAGTGT TTTTCTGTCA GCCAGAAGGA 
TGCCTGAGTG TCCGGCAGAA ACGCATGAGC CTGCGCGATG ACATGTCCTT TGTTTTCACT 
CTGACCGACA AAGATAGTGG TGTCATTCGA TATGGAATCT GCGTCAATTT CTACCGCTCT 
TTCCAGAAAC GCCTACCAAA GGACAAGAGC CGTAAACCCC CAAAGCCAGC ATCCGAAAAT 
GGTTCTGAGG GATCCGATGG AGGCCATCCC ATGCAGCCCC ATAGCGCTGA ATCCACCCCT 
GATATCAATC AATCTCCCCG TGGGAAACGT CTTTCTAAAG GGATGCAAAG GTCCCGGAAC 
AGTACATTGA CCTCGCTTTG CATCATCAGT CACTATCCTT TCTTTTCAAC ATTCCGAGAA 
TGTCTGTACA CCTTGAAGCG GTTAGTTGAC TGCTGCAGTG AGAGACTAGT TAGTAAGAAG 
GCTGGTGTCA CCAAAGGAGT TCAGAGAGAT ACCATGTGGA GGATCTTCAC CGGCTCCCTG 
CAGCTGGAGG AAAAACCCAA CGTGCTCCTG CATGACCTCC GAGAAATAGA AGCATGGATC 
TACCGCTTGT TGCGCTCCCC CACTCCGGTT CCAGGCCAGA AGCGAGTTGA TGTGGAGGTC 
CTGCCTCTCG ATCACCAACC ACCCCTGACC TTTGCCCTTC CTGATCCCTC GCGGTTCTCA 
CTGGTGGATT TCCCCTTGCA CCTGCCCTTG GAACTTCTTG GGGTGGAGGC GTGCTTGCAG 
GTTCTGTCCT GCATCATGTT GGAACACAAG GTGGTCATGC AGTCCCGCGA CTACAACGCG 
CTGTCTATGT CTGTGATGGC GTTTGTGTCT ATGATTTACC CACTTGAATA TATGTTTCCT 
GTTATTCCTC TTCTGCCCAC TTGCATGGCT TCTGCCGAAC AGCTGCTTCT CGCCCCCACC 
CCATACATAA TCGGGGTCCC TGCCAGCTTC TTTCTCTACA AGTCGGACTT TAAGATGCCA 
GATGACGTGT GGCTGGTTGA TCTTGACACT AATAAGGTGG TTGCTCCCAC CAATGCAGAC 
CTGTTGCCTG TTCTTCCAGA ACCAGAGGCC TCAGAACTGA AGAAGCACCT TAAACAGGCA 
TTGGCTAGCA TGAGTCTGAA CACTCAGCCT ATCCTCAATT TGGACCGATT TCACGAAGGC 
CCAGACGCTG CACTGTTGAA AGCCCCCAGT GATGCACAGG GAACTCCCTC CACAGAATTT 
AATCCTCTTA TATATGGAAA TGATGTTGAC TCTGTGGATG TGGCAACAAG GGTTGCCATG 
GTACGTTTCT TTAACTCCCC GAATATGTTG CACAGTTTCC AAATGCACAC ACGCACCCTG 
CGCCTTTTCC CTCGTCCAGT CGTGGCTTTC CAATCCTCTT CCTTCCTGGC AGCACGGCCC 
AAGCCCTCCC TTTTTACTGA GAAGCTTTCC AAAACGCAGG CAGTGGAATT CTTTGGGGAG 
TGGTCCCTGA ACCCCACTAA CTTTGCATTC CAGAGAATCC ACAACAACAT GTTTGACCCT 
GCTCTGATTG GGGATAAGCC CAAGTGGTAT GCACACCAGC TGCAGCCCAT TCACTACCGT 
GTATATGATG CTAACTCACA GCTTGCGGAA GCATTTTGTG TGCCAGCAGA GAGGGACAGT 
GACTCTGATC CTACTGATGA CAGTGGCAGC GATAGTGTGG AATATGATGA CTCCAGCTCT 
TCCTACTCTT CTCTTGGAGA CTTTGTCAGC GAAATGATGA AATGTGATAT TAATGGGGAC 
ACTCCAAATG TAGATCCGCT GACGCATGCA GCGCTTGGAG ATGCCAGTGA GGTTGAATTT 
GATGACTTCC AAGAATACTC AGGAGAAATA GAGGAGAACA ATGGCGATAG CGAGAACTCA 
CAAGATAACA ACCAGCCTCG CTCTGGATCT AGCACCACAG CCAGCAGCAG CCCCAGTACC 
GTTATACACG GGGCTAACAC GGAATCAGCC GATTCTGCAG AGCCCGAAGA GAAAGTTATG 
GGTGTGTTGC AAAATCACTT GCCAGTTTTG CCACCCACAT TCACCCGGTT AGCCATGGAG 
AGGCGGGAGA GTGATGTGTC ACTGGGTGGC GGAGAGGCAC GAAGACGAGA GTATGACAAC 
CCCTACTTTG AGCCACAGTA TGGATTTCCT CCAGAGGAGG AGAATGATGA ACGGGAGGAG 
AGCTATACAC CGATGTTTAA CCAGAGTGTG AATGGTACCC GATCCCCGAA GCCTCTGCTT 
CGGCCCAATA GCTTGAAGCT TGCAAGTGAT TCTGATGCCG AGACAGATTC TCGGGGTAGT 
TCTCCCACGT CAACTGTGTC CAACAACAGC AGCAATAACG AAGGCTTTGG AGGCATCATG 
TCCTTCGCCA GCAACCTGTA TAAAACTCAC AGCAGTAGCT TTAGCTTATC GAGCCTGACG 
CTGCCGAGCA AAAGCCGAGA CAAGGCCACT CCCTTCCCCA GCCTGAAAGG CAGTAACAGA 
CGTGCTCTTG TTGACCAGAA GTCTTCAGGT ATAAAGCACA GCCCTACAGT CAAGCGAGAA 
TCTCCCTCTC CTCAGGGAAA ATCAAACAAC TCCAGTGAGA ACCAGCAGTT CCTAAAGGAG 
GTGGCGCTGG GTGTTCTGGA GGGCCAAGGG GTAGGATGGC TGAACATGAA AAAGGTGCGG 
CGATTGCTGG AGAGCGAGCA GCTGCGCGTA TTTGTGCTAA GCAAATTGAA CAGGTCCATC 
CAGACAGAAG AAGATGCTCG CAATGATGTC ATCCAGGAAG TGGAAATAAC CCGAAAAGTG 
TATAGGGGCA TGCTGGACCT GCTGAAGTGC ACCGTATCGA GCCTCGAGCA GACTTACGCC 
AATGCTGGCC TGGGAGGAAT GGCCAGTGTC TTCAGCTTAC TCGAGATTGC TCATACTCAC 
TATTACAACA AAGAACCGGA AAAAAGGAAG CACAGTCCGT CTGCCGATGG AGCTGTCACC 
CCTGTGTCTA GGGATCCTGT CTCAGCTCCG AGGGTAGAGT CTCGACCCGC CCCTCAGCTG 
CCTGCCAAGC AGCTCTTGCC CCGCCCATCA GGCCCCGCTG GCAGAGGGGC ACGCGAGTTT 
GACACCCGGA GTCTTAAAGA AGAGAATTTT GTGGCATCCA TTGGTACCAA GCCAGCACCG 
CCTTTGGTTG ATAGTGGGGA AACAGAGGAA AAGAAGTCCC AGATTAGCGC CGATAGTGGC 
CTGAGCGTCA CATCTGGTTC TCAGCGAAGC GATCTAGACA CAGGCAGCAT AGGACCCTCC 
ATCCTTATCC GAAGTAGCAG CCAGGATTCG GAAATCAGCA CAGTGGTGAG TAACAGCTCA 
GGGGAAACAC TGGGAGCCGA CAGCGACTTA AGTAGCAACG CAGGGGATGC CTTGGGCGGC 
CCAGGAGGAA GCCACCTCTC GGGATCCAGA GGGGCTGTGT CCGACAGCGA GATTCAGATG 
AACTCATTCA CCGGAGCCAT CTTTGGAAAG CCTCAGATGC TCCGCCCTGG TGAGAAAGAA 
AGGAATGTGA GTCCTGGGCG CCCCCAGCAA GACTTGAGCC AACGCATATA CCTGTATGAG 
GGGCTTTTAG GTAAGGAGCG TTCCACTCTG TGGGACCAGA TGCAGTTTTG GGAGGATGCT 
TTCCTAGACG CTGTTATGCT GGAGAGAGAA GGAATGGGTA TGGACCAAGG CCCACAGGAG 
ATGATAGACA GGTATTTCTC TCTTGGCGAC CATGACCGGA AGCGCTTAGA GGATGATGAG 
GATCGCCTGC TGGCCACTCT TTTGCACAAT ATGATTGCCT ATATGATCAT GATGAAGGTG 
AATAAGACAG ATCTACGCAG GAAGGTCAGG CGTATCCTTG GAAAGTGCCA CATAGGCTTA 
GTGCACAGCC AACAAATTAA TGAACTGCTG GACAGGGTGA CTGACATGAA TGGCCGCGAT 
CTCAGCATTC GTCCCAGTGG GAGCCGTCAC ATCAAGAAGC AAACCTTTGT AGTGCATGCT 
GGGACAGACA CAAGTGGGGA CCTTTTCTTT ATGGAGGTTT GTGACGACTG CATCGTCCTT 
CGCAGCAACA TCGGTGCCGT GTATGAACGT TGGTGGTACG AAAAACTTAT TAACATGACT 
TATTGTCCCA AGACTAAAGT GCTCTGTCTG TGGAGGAGAA ATGGCCAAGA AACTCAGCTC 
AACAAATTCT TCACCAAGAA GTGCCGGGAG CTGTACTACT GTGTGAAGGA GAGCATGGAA 
CGGGCGGCAG CACGACAGCA GAGCATAAAG CCAGGGCCGG AACTTGGCGG AGAGTTTCCT 
GTCCAGGACA TGAAGACAGG AGAAGGGGGT CTTCTTCAAG TTACCTTGGA GGGAATTAAC 
CTAAAATTCA TGCACAGTCA GTTCCTGAAA CTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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madd cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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