agl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following agl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa44199 XM_018093624.1
Latest version!
Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa57297 XM_031901014.1
Latest version!
Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57297 XM_031901015.1
Latest version!
Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57297 XM_031901012.1
Latest version!
Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57297 XM_031901016.1
Latest version!
Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57298 XM_031901017.1
Latest version!
Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa44199
    Clone ID Related Accession (Same CDS sequence) XM_018093624.1
    Accession Version XM_018093624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product glycogen debranching enzyme
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GGAGATGCTG
    GCTGCAGCCA GGTCTGTCAG ACCAAATCTT TATGTGATTG CTGAGCTCTT CACTGGCAGC
    GAATACATCG ACAATGTGTT TGTCAACCGC CTGGGAATTA CAAGCCTCAT TCGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT AAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949113.1
    CDS565..5163
    Translation

    Target ORF information:

    RefSeq Version XM_018093624.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GGAGATGCTG
    GCTGCAGCCA GGTCTGTCAG ACCAAATCTT TATGTGATTG CTGAGCTCTT CACTGGCAGC
    GAATACATCG ACAATGTGTT TGTCAACCGC CTGGGAATTA CAAGCCTCAT TCGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT AAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57297
    Clone ID Related Accession (Same CDS sequence) XM_031901014.1 , XM_031901015.1 , XM_031901012.1 , XM_031901016.1
    Accession Version XM_031901014.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG
    GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT
    GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756874.1
    CDS188..4786
    Translation

    Target ORF information:

    RefSeq Version XM_031901014.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG
    GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT
    GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57297
    Clone ID Related Accession (Same CDS sequence) XM_031901014.1 , XM_031901015.1 , XM_031901012.1 , XM_031901016.1
    Accession Version XM_031901015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG
    GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT
    GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756875.1
    CDS516..5114
    Translation

    Target ORF information:

    RefSeq Version XM_031901015.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG
    GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT
    GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57297
    Clone ID Related Accession (Same CDS sequence) XM_031901014.1 , XM_031901015.1 , XM_031901012.1 , XM_031901016.1
    Accession Version XM_031901012.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG
    GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT
    GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756872.1
    CDS174..4772
    Translation

    Target ORF information:

    RefSeq Version XM_031901012.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG
    GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT
    GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57297
    Clone ID Related Accession (Same CDS sequence) XM_031901014.1 , XM_031901015.1 , XM_031901012.1 , XM_031901016.1
    Accession Version XM_031901016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG
    GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT
    GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756876.1
    CDS309..4907
    Translation

    Target ORF information:

    RefSeq Version XM_031901016.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG
    GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT
    GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57298
    Clone ID Related Accession (Same CDS sequence) XM_031901017.1
    Accession Version XM_031901017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product glycogen debranching enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GGAGATGCTG
    GCTGCAGCCA GGTCTGTCAG ACCAAATCTT TATGTGATTG CTGAGCTCTT CACTGGCAGC
    GAATACATCG ACAATGTGTT TGTCAACCGC CTGGGAATTA CAAGCCTCAT TCGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756877.1
    CDS174..4772
    Translation

    Target ORF information:

    RefSeq Version XM_031901017.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
    AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA
    GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG
    ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC
    TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG
    AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA
    GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG
    GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC
    ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG
    CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG
    GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC
    ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT
    TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT
    GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA
    CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG
    TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT
    GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA
    TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC
    AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG
    CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT
    TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG
    AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT
    GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC
    TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT
    CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT
    GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC
    CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GGAGATGCTG
    GCTGCAGCCA GGTCTGTCAG ACCAAATCTT TATGTGATTG CTGAGCTCTT CACTGGCAGC
    GAATACATCG ACAATGTGTT TGTCAACCGC CTGGGAATTA CAAGCCTCAT TCGAGAGGCC
    ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT
    GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT
    TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA
    CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT
    GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG
    AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT
    GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG
    TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC
    CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC
    AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA
    ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC
    CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA
    GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG
    ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA
    GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA
    GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT
    CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT
    GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA
    TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG
    AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA
    TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT
    TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT
    GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG
    GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA
    CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC
    TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA
    AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC
    AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG
    GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT
    ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC
    AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG
    TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG
    AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA
    GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG
    GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT
    GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG
    AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG
    ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT
    GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT
    ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC
    TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT
    CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT
    GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA
    AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT
    GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG
    GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC
    CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG
    TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.