prune2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following prune2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prune2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24760 NM_001079124.2
Latest version!
Xenopus tropicalis prune homolog 2 (prune2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OXa48045 XM_031901877.1
Latest version!
Xenopus tropicalis prune homolog 2 (prune2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OXa48040 XM_031901853.1
Latest version!
Xenopus tropicalis prune homolog 2 (prune2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa48041 XM_031901857.1
Latest version!
Xenopus tropicalis prune homolog 2 (prune2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa48042 XM_031901864.1
Latest version!
Xenopus tropicalis prune homolog 2 (prune2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa48043 XM_031901872.1
Latest version!
Xenopus tropicalis prune homolog 2 (prune2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa48044 XM_031901874.1
Latest version!
Xenopus tropicalis prune homolog 2 (prune2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
View Old Accession Versions >>
OXa24760 NM_001079124.1 Xenopus tropicalis prune homolog 2 (prune2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24760
    Clone ID Related Accession (Same CDS sequence) NM_001079124.2 , NM_001079124.1
    Accession Version NM_001079124.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 951bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein prune homolog 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC122960.1. On May 2, 2019 this sequence version replaced NM_001079124.1. ##Evidence-Data-START## Transcript exon combination :: BC122960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01758126 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
    AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC
    CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT
    AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT
    GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA
    GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG
    CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC
    TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC
    CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG
    CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA
    ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG
    AATTTAAAGT CTTTTATAAT TGTTCATCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG
    ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA
    GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAACTG
    GATGAAGAAT TGAGAGAAAG TGAATCTCCC AAAGCTGGCT GTCTTCCCAA TGAACCTGAA
    ATGAATACCT TGGAGGAGGA ATTTGAAAAC AAGATGGGAG ACAACGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072592.1
    CDS120..1070
    Translation

    Target ORF information:

    RefSeq Version NM_001079124.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis prune homolog 2 (prune2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079124.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
    AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC
    CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT
    AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT
    GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA
    GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG
    CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC
    TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC
    CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG
    CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA
    ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG
    AATTTAAAGT CTTTTATAAT TGTTCATCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG
    ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA
    GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAACTG
    GATGAAGAAT TGAGAGAAAG TGAATCTCCC AAAGCTGGCT GTCTTCCCAA TGAACCTGAA
    ATGAATACCT TGGAGGAGGA ATTTGAAAAC AAGATGGGAG ACAACGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa24760
    Clone ID Related Accession (Same CDS sequence) NM_001079124.2 , NM_001079124.1
    Accession Version NM_001079124.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 951bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-29
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein prune homolog 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC122960.1. ##Evidence-Data-START## Transcript exon combination :: BC122960.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00861994, SAMN00861995 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
    AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC
    CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT
    AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT
    GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA
    GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG
    CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC
    TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC
    CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG
    CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA
    ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG
    AATTTAAAGT CTTTTATAAT TGTTCATCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG
    ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA
    GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAACTG
    GATGAAGAAT TGAGAGAAAG TGAATCTCCC AAAGCTGGCT GTCTTCCCAA TGAACCTGAA
    ATGAATACCT TGGAGGAGGA ATTTGAAAAC AAGATGGGAG ACAACGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072592.1
    CDS120..1070
    Translation

    Target ORF information:

    RefSeq Version NM_001079124.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis prune homolog 2 (prune2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079124.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
    AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC
    CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT
    AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT
    GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA
    GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG
    CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC
    TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC
    CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG
    CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA
    ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG
    AATTTAAAGT CTTTTATAAT TGTTCATCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG
    ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA
    GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAACTG
    GATGAAGAAT TGAGAGAAAG TGAATCTCCC AAAGCTGGCT GTCTTCCCAA TGAACCTGAA
    ATGAATACCT TGGAGGAGGA ATTTGAAAAC AAGATGGGAG ACAACGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48045
    Clone ID Related Accession (Same CDS sequence) XM_031901877.1
    Accession Version XM_031901877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein prune homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
    AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC
    CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT
    AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT
    GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA
    GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG
    CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC
    TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC
    CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG
    CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA
    ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG
    AATTTAAAGT CTTTTATAAT TGTTCACCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG
    ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA
    GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAGTAT
    GAAGAATCAA GATGTTTTCC AAGAACTGTG AGACTGGATG AAGAATTGAG AGAAAGTGAA
    TCTCCCAAAG CTGGCTGTCT TCCCAATGAA CCTGAAATGA ATACCTTGGA GGAGGAATTT
    GAAAACAAGA TGGGAGACAA CGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757737.1
    CDS191..1177
    Translation

    Target ORF information:

    RefSeq Version XM_031901877.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis prune homolog 2 (prune2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
    AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC
    CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT
    AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT
    GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA
    GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG
    CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC
    TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC
    CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG
    CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA
    ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG
    AATTTAAAGT CTTTTATAAT TGTTCACCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG
    ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA
    GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAGTAT
    GAAGAATCAA GATGTTTTCC AAGAACTGTG AGACTGGATG AAGAATTGAG AGAAAGTGAA
    TCTCCCAAAG CTGGCTGTCT TCCCAATGAA CCTGAAATGA ATACCTTGGA GGAGGAATTT
    GAAAACAAGA TGGGAGACAA CGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48040
    Clone ID Related Accession (Same CDS sequence) XM_031901853.1
    Accession Version XM_031901853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein prune homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT
    TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT
    ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA
    TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAGTATGA AGAATCAAGA
    TGTTTTCCAA GAACTGTGAG ACTGGATGAA GAATTGAGAG AAAGTGAATC TCCCAAAGCT
    GGCTGTCTTC CCAATGAACC TGAAATGAAT ACCTTGGAGG AGGAATTTGA AAACAAGATG
    GGAGACAACG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757713.1
    CDS12..7166
    Translation

    Target ORF information:

    RefSeq Version XM_031901853.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis prune homolog 2 (prune2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT
    TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT
    ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA
    TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAGTATGA AGAATCAAGA
    TGTTTTCCAA GAACTGTGAG ACTGGATGAA GAATTGAGAG AAAGTGAATC TCCCAAAGCT
    GGCTGTCTTC CCAATGAACC TGAAATGAAT ACCTTGGAGG AGGAATTTGA AAACAAGATG
    GGAGACAACG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48041
    Clone ID Related Accession (Same CDS sequence) XM_031901857.1
    Accession Version XM_031901857.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein prune homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT
    TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT
    ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA
    TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAACTGGA TGAAGAATTG
    AGAGAAAGTG AATCTCCCAA AGCTGGCTGT CTTCCCAATG AACCTGAAAT GAATACCTTG
    GAGGAGGAAT TTGAAAACAA GATGGGAGAC AACGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757717.1
    CDS12..7130
    Translation

    Target ORF information:

    RefSeq Version XM_031901857.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis prune homolog 2 (prune2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901857.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT
    TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT
    ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA
    TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAACTGGA TGAAGAATTG
    AGAGAAAGTG AATCTCCCAA AGCTGGCTGT CTTCCCAATG AACCTGAAAT GAATACCTTG
    GAGGAGGAAT TTGAAAACAA GATGGGAGAC AACGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48042
    Clone ID Related Accession (Same CDS sequence) XM_031901864.1
    Accession Version XM_031901864.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein prune homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT
    TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT
    ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA
    TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAGTATGA AGAATCAAGA
    TGTTTTCCAA GAACTGTGAG AGCTGGCTGT CTTCCCAATG AACCTGAAAT GAATACCTTG
    GAGGAGGAAT TTGAAAACAA GATGGGAGAC AACGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757724.1
    CDS12..7130
    Translation

    Target ORF information:

    RefSeq Version XM_031901864.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis prune homolog 2 (prune2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901864.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT
    TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT
    ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA
    TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAGTATGA AGAATCAAGA
    TGTTTTCCAA GAACTGTGAG AGCTGGCTGT CTTCCCAATG AACCTGAAAT GAATACCTTG
    GAGGAGGAAT TTGAAAACAA GATGGGAGAC AACGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48043
    Clone ID Related Accession (Same CDS sequence) XM_031901872.1
    Accession Version XM_031901872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein prune homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT
    TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT
    ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA
    TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAAGCTGG CTGTCTTCCC
    AATGAACCTG AAATGAATAC CTTGGAGGAG GAATTTGAAA ACAAGATGGG AGACAACGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757732.1
    CDS12..7094
    Translation

    Target ORF information:

    RefSeq Version XM_031901872.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis prune homolog 2 (prune2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT
    TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT
    ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA
    TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAAGCTGG CTGTCTTCCC
    AATGAACCTG AAATGAATAC CTTGGAGGAG GAATTTGAAA ACAAGATGGG AGACAACGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48044
    Clone ID Related Accession (Same CDS sequence) XM_031901874.1
    Accession Version XM_031901874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein prune homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGAG CTGGCTGTCT TCCCAATGAA
    CCTGAAATGA ATACCTTGGA GGAGGAATTT GAAAACAAGA TGGGAGACAA CGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757734.1
    CDS12..6908
    Translation

    Target ORF information:

    RefSeq Version XM_031901874.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis prune homolog 2 (prune2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
    ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT
    GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG
    AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC
    CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC
    AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT
    AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC
    CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA
    AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT
    ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT
    GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT
    GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT
    GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT
    GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA
    GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT
    ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA
    AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA
    AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT
    AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG
    GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT
    GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT
    AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT
    GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT
    TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC
    TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA
    TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA
    ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA
    AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA
    TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT
    GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC
    ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT
    TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT
    TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA
    AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA
    AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA
    AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA
    AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA
    AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC
    CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT
    GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA
    CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT
    ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG
    CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC
    CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA
    AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA
    CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT
    GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT
    GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT
    CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT
    CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG
    GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA
    GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA
    AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT
    AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG
    TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG
    AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA
    GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA
    CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG
    GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA
    TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC
    TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA
    GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG
    GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA
    CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC
    CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT
    GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT
    TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT
    TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC
    CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT
    GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT
    TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG
    AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC
    AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA
    GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA
    ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA
    GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC
    TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT
    GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT
    GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA
    CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC
    AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT
    GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG
    AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC
    AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC
    AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA
    GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC
    AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG
    GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT
    GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT
    GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT
    CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT
    TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT
    TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA
    ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC
    TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC
    CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG
    TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA
    TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA
    GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA
    GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA
    ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC
    CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA
    GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT
    CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC
    ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA
    TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT
    CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA
    CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA
    AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT
    ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT
    GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC
    AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA
    GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA
    GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGAG CTGGCTGTCT TCCCAATGAA
    CCTGAAATGA ATACCTTGGA GGAGGAATTT GAAAACAAGA TGGGAGACAA CGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.